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[research advances in cadmium pollution of peanut (arachis hypogaea l.)].peanut (arachis hypogaea l.) is a major oil-bearing crop in the world, and as well, an important resource of plant protein and a main raw material for food processing. with the increasing of its direct human consumption and food processing, the cd concentration in peanut kernel has aroused great concern in recent years. china is a main country of the production and exportation of peanut, but the cd enrichment in peanut kernel is the main obstacle for its peanut export trade. in this paper, the r ...200819288735
impact of filter mud applications on the germination of sugarcane and small-seeded plants and on soil and sugarcane nitrogen contents.the impact of filter mud (fm) on the germination of sugarcane (saccharum spp.), guar (cyamopsis tetragonoloba), maize (zea mays), groundnut (arachis hypogaea) and sesame (sesamum indicum), and the nitrogen content of soil and sugarcane were investigated. experiments were set up in 2004 at the kenana sugarcane estate, sudan. for this study, 1:0,3:1,1:1,1:3 and 0:1 fm/soil (w/w) mixtures were used. the results showed that fm had no adverse impact on germination. soil organic carbon and total nitro ...200817945488
comparison of the key aroma compounds in organically grown, raw west-african peanuts (arachis hypogaea) and in ground, pan-roasted meal produced thereof.application of an aroma extract dilution analysis on an aroma distillate prepared from organically grown, raw west-african peanuts (cameroon) revealed 36 odor-active areas in the flavor dilution (fd) factor range of 1 to 2048. the identification experiments, which were all performed by using the respective reference chemicals, revealed 2-isopropyl-3-methoxypyrazine (earthy, pea-like), 2-isobutyl-3-methoxypyrazine (bell pepper-like, earthy), and trans-4,5-epoxy-(e)-2-decenal (metallic) with the h ...200818939847
adsorption of peanut (arachis hypogaea, leguminosae) proteins by activated charcoal.the binding of peanut protein allergens to activated charcoal (ac), used medically for gastric decontamination following the ingestion of toxic substances, was investigated for potential clinical application. crude peanut extract (cpe) or purified peanut protein allergens ara h 1 and 2 were co-incubated with ac under a variety of conditions followed by centrifugation to remove the ac and adsorbed protein. the resulting supernatant solution was analyzed for unadsorbed protein by gel electrophores ...200818983155
development and use of single nucleotide polymorphism markers for candidate resistance genes in wild peanuts (arachis spp).the cultivated peanut (arachis hypogaea l.) is an allotetraploid of recent origin, with an aabb genome and low genetic diversity. perhaps because of its limited genetic diversity, this species lacks resistance to a number of important pests and diseases. in contrast, wild species of arachis are genetically diverse and are rich sources of disease resistance genes. consequently, a study of wild peanut relatives is attractive from two points of view: to help understand peanut genetics and to charac ...200818752190
identification of galectin-3 and mucin-type o-glycans in breast cancer and its metastasis to brain.galectin-3 has been implicated in tumor progression. we demonstrated immunohistochemically that galectin-3 was negative in normal breast tissue, but it was highly increased in breast cancer and in metastatic tissues to brain. similarly, histochemistry with mucin-specific lectins showed increased recognition in breast tumor and metastasis with machaerocereus eruca agglutinin (fualpha 1,2 (galnacalpha 1,3) galss1,4 in complex mucin) but not for amaranthus leucocarpus (galss1,3-galnac-alpha 1,0-ser ...200818584353
utilization of arachis hypogaea hull, an agricultural waste for the production of activated carbons to remove phenol from aqueous solutions.arachis hypogaea hulls, an agricultural waste, were used to prepare activated carbon by chemical activation with zinc chloride under four different activation atmospheres. the most important parameter in chemical activation was found to be the chemical ratio (activating agent/precursor). carbonization temperature and time are the other two important variables, which had significant effect on the pore structure of carbon. the maximum brunquer-emmett-teller (bet) surface area and micropore volume ...200818576227
isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (arachis hypogaea).cultivated peanut or groundnut (arachis hypogaea l.) is the fourth most important oilseed crop in the world, grown mainly in tropical, subtropical and warm temperate climates. due to its origin through a single and recent polyploidization event, followed by successive selection during breeding efforts, cultivated groundnut has a limited genetic background. in such species, microsatellite or simple sequence repeat (ssr) markers are very informative and useful for breeding applications. the low le ...200818482440
spermidine and flavonoid conjugates from peanut (arachis hypogaea) flowers.a new spermidine triamide derivative has been isolated from peanut flowers and identified as n (1)-acetyl- n (5), n (10)-di- p-( ee)-coumaroylspermidine on the basis of detailed analysis of nmr, ms, and uv data. two other spermidine conjugates, n (1), n (5), n (10)-tri- p-( eee)-coumaroylspermidine and di- p-( ee)-coumaroylspermidine, as well as four flavonoid conjugates (quercetin-3-glucoside, quercetin-3-glucuronide, isorhamnetin-3-glucoside, and isorhamnetin-3-glucuronide) that have been prev ...200818412366
developmental changes in peanut root structure during root growth and root-structure modification by nodulation.basic information about the root and root nodule structure of leguminous crop plants is incomplete, with many aspects remaining unresolved. peanut (arachis hypogaea) forms root nodules in a unique process. structures of various peanut root types were studied with emphasis on insufficiently characterized lateral roots, changes in roots during their ontogenesis and root modification by nodule formation.200818256023
bac libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut.cultivated peanut, arachis hypogaea is an allotetraploid of recent origin, with an aabb genome. in common with many other polyploids, it seems that a severe genetic bottle-neck was imposed at the species origin, via hybridisation of two wild species and spontaneous chromosome duplication. therefore, the study of the genome of peanut is hampered both by the crop's low genetic diversity and its polyploidy. in contrast to cultivated peanut, most wild arachis species are diploid with high genetic di ...200818230166
utilizing peanut husk (arachis hypogaea l.) in the manufacture of medium-density fiberboards.the main objective of this study was to investigate the potential of peanut husk (arachis hypogaea l.) as a fiber-peanut mixture to produce fiberboards for general purposes. for panel production, the addition of peanut husk at various percentages to the wood fiber was the only variable. panels produced utilizing peanut husk were compared to panels produced using 100% wood fiber. the chemical properties of peanut husk; holocellulose and lignin content, alcohol-benzene, hot and cold water, and dil ...200818063362
effect of sewage water on seed germination and vigour index of different varieties of groundnut (arachis hypogaea l.).the study has been focused on the investigation on ground nut (arachis hypogea) fields influenced by sewage water. sewage water sampled in and around mysore city and analyzed forphysicochemical parameters. different concentrations such as 1, 5, 10, 25, 50, 70 and 100% of sewage water on seed germination and vigour index of arachis hypogea verities such as dh - 2 - 30, icjs - 11, jl - 24, k - 134, tmv - 2 and vri - 2 were studied. from the recorded observation it is concluded that the sewage wate ...200819297996
antimicrobial and antioxidant activities of cortex magnoliae officinalis and some other medicinal plants commonly used in south-east asia.eight medicinal plants were tested for their antimicrobial and antioxidant activities. different extraction methods were also tested for their effects on the bioactivities of the medicinal plants.200819038060
application of natural adsorbent from silver impregnated arachis hypogaea based thereon in the processes of hexavalent chromium for the purification of water.different industries generate huge amounts of chromium, both in solid and liquid form which leached out and find their way to the aquifers. here we report the removal of cr(vi) by the silver impregnated activated carbon developed from agricultural waste material at ph 3 from drinking water. result revealed that adsorption of chromium(vi) on silver impregnated groundnut husk carbon was endothermic in nature. thermodynamic parameters such as the entropy change, enthalpy change and gibbs free energ ...200919026487
identification and characterization of a hypoallergenic ortholog of ara h 2.01.peanut (arachis hypogaea l.), can elicit type i allergy becoming the most common cause of fatal food-induced anaphylactic reactions. strict avoidance is the only effective means of dealing with this allergy. ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognized by approximately 90% of peanut hypersensitive individuals in the us. because peanut has limited genetic variation, wild relatives are a good source of genetic diversity. after scr ...200919009240
a linkage map for the b-genome of arachis (fabaceae) and its synteny to the a-genome.arachis hypogaea (peanut) is an important crop worldwide, being mostly used for edible oil production, direct consumption and animal feed. cultivated peanut is an allotetraploid species with two different genome components, a and b. genetic linkage maps can greatly assist molecular breeding and genomic studies. however, the development of linkage maps for a. hypogaea is difficult because it has very low levels of polymorphism. this can be overcome by the utilization of wild species of arachis, w ...200919351409
synthesis and biological evaluation of multivalent carbohydrate ligands obtained by click assembly of pseudo-rotaxanes.multivalent carbohydrate ligands have been prepared by assembling alpha-cyclodextrin-based pseudo-rotaxanes through "click chemistry". the inclusion complex formed by a lactosyl-alpha-cd conjugate and a decane axle carrying a lactosyl stopper at one extremity and an azido group at the other end was dimerized by bis-propargyl spacers of different lengths to provide oligorotaxanes having adjustable threading ratios. for the first time, saccharidic ligands have been introduced on rotaxanes both as ...200919343257
utility of est-derived ssr in cultivated peanut (arachis hypogaea l.) and arachis wild species.lack of sufficient molecular markers hinders current genetic research in peanuts (arachis hypogaea l.). it is necessary to develop more molecular markers for potential use in peanut genetic research. with the development of peanut est projects, a vast amount of available est sequence data has been generated. these data offered an opportunity to identify ssr in ests by data mining.200919309524
commercial lateral flow devices for rapid detection of peanut (arachis hypogaea) and hazelnut (corylus avellana) cross-contamination in the industrial production of cookies.lateral flow devices (lfds) are qualitative immunochromatographic tests for the rapid and specific detection of target analytes. we investigated commercially available lfds for their ability to detect potentially allergenic peanut and hazelnut in raw cookie dough and chocolate, two important food matrices in the industrial production of cookies. each three commercial lfds for the detection of hazelnut and peanut were performed according to the manufacturers' instructions. all lfds had comparably ...200919280180
determination of amino acid content and protein quality of complementary food produced from locally available food materials in ondo state, nigeria.protein-energy malnutrition is increasing among children in developing countries due to low nutrient density of traditional complementary diets. therefore, this study aimed at determining the protein quality of a complementary food produced from cooking banana fruits and bambara groundnut seeds. the cooking banana and bambara groundnut seeds are locally available in both urban and villages markets in nigeria. the cooking bananas (cb) and bambara groundnut (bg) seeds were processed into flours us ...200922691808
molecular biogeographic study of recently described b- and a-genome arachis species, also providing new insights into the origins of cultivated peanut.the cultivated peanut arachis hypogaea is a tetraploid, likely derived from a- and b-genome species. reproductive isolation of the cultigen has resulted in limited genetic variability for important traits. artificial hybridizations using selected diploid parents have introduced alleles from wild species, but improved understanding of recently classified b-genome accessions would aid future introgression work. to this end, 154 cdna probes were used to produce 1887 rflp bands scored on 18 recently ...200919234559
an analysis of synteny of arachis with lotus and medicago sheds new light on the structure, stability and evolution of legume genomes.most agriculturally important legumes fall within two sub-clades of the papilionoid legumes: the phaseoloids and galegoids, which diverged about 50 mya. the phaseoloids are mostly tropical and include crops such as common bean and soybean. the galegoids are mostly temperate and include clover, fava bean and the model legumes lotus and medicago (both with substantially sequenced genomes). in contrast, peanut (arachis hypogaea) falls in the dalbergioid clade which is more basal in its divergence w ...200919166586
the first ssr-based genetic linkage map for cultivated groundnut (arachis hypogaea l.).molecular markers and genetic linkage maps are pre-requisites for molecular breeding in any crop species. in case of peanut or groundnut (arachis hypogaea l.), an amphidiploid (4x) species, not a single genetic map is, however, available based on a mapping population derived from cultivated genotypes. in order to develop a genetic linkage map for tetraploid cultivated groundnut, a total of 1,145 microsatellite or simple sequence repeat (ssr) markers available in public domain as well as unpublis ...200919048225
rna interference highlights the role of ccamk in dissemination of endosymbionts in the aeschynomeneae legume arachis.in legume-rhizobia symbiosis, ca2+/calmodulin-dependent protein kinase (ccamk) is essential for rhizobial invasion through infection threads in the epidermis and nodule organogenesis in the cortex. though ccamk is actively transcribed in the infected zone of nodules, its role in the later stages of nodule development remain elusive because of the epidermal arrest of "loss-of-function" mutants. in aeschynomeneae legumes such as arachis hypogea, rhizobia directly access the cortex, where nodule or ...200919810815
assessment of transpiration efficiency in peanut (arachis hypogaea l.) under drought using a lysimetric system.transpiration efficiency (te) is an important trait for drought tolerance in peanut (arachis hypogaea l.). the variation in te was assessed gravimetrically using a long time interval in nine peanut genotypes (chico, icgs 44, icgv 00350, icgv 86015, icgv 86031, icgv 91114, jl 24, tag 24 and tmv 2) grown in lysimeters under well-watered or drought conditions. transpiration was measured by regularly weighing the lysimeters, in which the soil surface was mulched with a 2-cm layer of polythene beads. ...200919778376
regulation of oleosin expression in developing peanut (arachis hypogaea l.) embryos through nucleosome loss and histone modifications.nucleosome loss and histone modifications are important mechanisms for transcriptional regulation. concomitant changes in chromatin structures of two peanut (arachis hypogaea l.) oleosin genes, aholeo17.8 and aholeo18.5, were examined in relation to transcriptional activity. spatial and temporal expression analyses showed that both aholeo17.8 and aholeo18.5 promoters can adopt three conformational states, an inactive state (in vegetative tissues), a basal activated state (in early maturation emb ...200919737778
cloning and characterization of the promoter of the 9-cis-epoxycarotenoid dioxygenase gene in arachis hypogaea l.we cloned the promoter of the 9-cis-epoxycarotenoid dioxygenase gene from arachis hypogaea l. beta-glucuronidase (gus) histochemical staining and gus activity assay indicated that the activity of the promoter was exhibited predominantly in the leaves and enhanced by water and nacl stresses, and by application of abscisic acid (aba) and salicylic acid (sa) in transgenic arabidopsis. moreover, two novel abre-like (abscisic acid response element) elements were identified in the promoter region.200919734653
genetic mapping of wild introgressions into cultivated peanut: a way toward enlarging the genetic basis of a recent allotetraploid.peanut (arachis hypogaea l.) is widely used as a food and cash crop around the world. it is considered to be an allotetraploid (2n = 4x = 40) originated from a single hybridization event between two wild diploids. the most probable hypothesis gave a. duranensis as the wild donor of the a genome and a. ipaënsis as the wild donor of the b genome. a low level of molecular polymorphism is found in cultivated germplasm and up to date few genetic linkage maps have been published. the utilization of wi ...200919650911
2-d dige analysis of the proteome of extracts from peanut variants reveals striking differences in major allergen contents.over the last decade, an increasing prevalence of peanut allergies was observed worldwide. peanuts are meanwhile categorized among the most dangerous food allergens. this is particularly relevant since peanut-derived ingredients are widely used in industrial food production. to minimize the problem of hidden food allergens causing severe anaphylactic reactions, pre-packaged food containing peanut components needs to be classified according to european ruling since 2005. food companies search for ...200919609960
[physiological responses of different peanut (arachis hypogaea l.) varieties to cadmium stress].to have a deep understanding on the mechanisms of cadmium (cd) toxicity on peanut plants is of theoretical and practical significances for the selection and utilization of cd-resistant peanut germ plasm resources. with fourteen peanut varieties as test materials and taking the chlorophyll content of functional leaves, malondialdehyde (mda) content and cell membrane permeability of roots and leaves, and oxidative vitality of roots at flowering stage as test physiological parameters, a sand cultur ...200919459390
goober: a fully integrated and user-friendly microarray data management and analysis solution for core labs and bench biologists.despite the large number of software tools developed to address different areas of microarray data analysis, very few offer an all-in-one solution with little learning curve. for microarray core labs, there are even fewer software packages available to help with their routine but critical tasks, such as data quality control (qc) and inventory management. we have developed a simple-to-use web portal to allow bench biologists to analyze and query complicated microarray data and related biological ...200920134074
peanut skin color: a biomarker for total polyphenolic content and antioxidative capacities of peanut cultivars.attempts to establish a relationship between peanut skin color (psc) and total flavonoid (tf) content have produced inconclusive results. this study investigated the potential of psc as a biomarker for polyphenol content and antioxidant capacity. peanut cultivars were objectively evaluated for their skin color, total phenolic (tp), flavonoid (tf), proanthocyanidin (tpc) contents and antioxidant capacities (ac). their relationship was determined by pearson's correlation analyses. tp had stronger ...200920087468
effect of frequency and duration of ultrasonication on the extraction efficiency of selected isoflavones and trans-resveratrol from peanuts (arachis hypogaea).plant derived phytochemicals have been the focus of recent research due to their health promoting effects. previous studies to estimate the levels of these bioactive compounds made use of traditional solvent extraction procedures such as homogenization and soxhlet (reflux) methods. recently, the ultrasonication technique has been shown to be an efficient non-thermal extraction method. the objective of this study was to investigate the effect of frequency and duration (time) of sonication on the ...200918849184
population dynamics of meloidogyne arenaria and pasteuria penetrans in a long-term crop rotation study.the endospore-forming bacterium pasteuria penetrans is an obligate parasite of root-knot nematodes (meloidogyne spp.). the primary objective of this study was to determine the effect of crop sequence on abundance of p. penetrans. the experiment was conducted from 2000 to 2008 at a field site naturally infested with both the bacterium and its host meloidogyne arenaria and included the following crop sequences: continuous peanut (arachis hypogaea) (p-p-p) and peanut rotated with either 2 years of ...200922736828
reproduction of meloidogyne marylandi and m. incognita on several poaceae.the susceptibility of 22 plant species to meloidogyne marylandi and m. incognita was examined in three greenhouse experiments. inoculum of m. marylandi was eggs from cultures maintained on zoysia matrella "cavalier" or cynodon dactylon x c. trasvaalensis "tifdwarf". inoculum of m. incognita was eggs from cultures maintained on solanum lycopersicum 'rutgers'. in each host test the inoculum density was 2,000 nematode eggs/pot. none of the three dicot species tested (gossypium hirsutum, arachis hyp ...200922661770
cadmium induced changes on proline, antioxidant enzymes, nitrate and nitrite reductases in arachis hypogaea l.the groundnut, arachis hypogaea seedlings, when grown in pot cultures for 10-25 days at 25, 50, 100 microm cdcl2, showed a marked decline in growth compared to control. similar trend was observed for nitrate reductase (nr) and nitrite reductase (nir) activities whereas proline, peroxidase (pod) and catalase (cat) showed increasing trend when observed on the 10th day of the experiment. changes have occurred in the physiological and biochemical activities which are observed even at low cd levels ( ...200920121033
endophytic occupation of peanut root nodules by opportunistic gammaproteobacteria.several bacterial isolates were recovered from surface-sterilized root nodules of arachis hypogaea l. (peanut) plants growing in soils from córdoba, argentina. the 16s rdna sequences of seven fast-growing strains were obtained and the phylogenetic analysis showed that these isolates belonged to the phylum proteobacteria, class gammaproteobacteria, and included pseudomonas spp., enterobacter spp., and klebsiella spp. after storage, these strains became unable to induce nodule formation in arachis ...200919054642
new stilbenoids from peanut ( arachis hypogaea ) seeds challenged by an aspergillus caelatus strain.four new stilbene derivatives, termed arahypins, have been isolated from peanut seeds challenged by an aspergillus caelatus strain, along with two known stilbenoids that have not been previously reported in peanuts. the structures of these new putative phytoalexins were determined by analysis of nmr, ms, and uv data. together with other known peanut stilbenoids that were also produced in the challenged seeds, these new compounds may play a defensive role against invasive fungi.200919063668
systematic determination of the peptide acceptor preferences for the human udp-gal:glycoprotein-alpha-galnac beta 3 galactosyltransferase (t-synthase).mucin-type protein o-glycosylation is initiated by the addition of alpha-galnac to ser/thr residues of a polypeptide chain. the addition of beta-gal to galnac by the udp-gal:glycoprotein-alpha-galnac beta 3 galactosyltransferase (t-synthase), forming the core 1 structure (beta-gal(1-3)-alpha-galnac-o-ser/thr), is a common and biologically significant subsequent step in o-glycan biosynthesis. what dictates the sites of core 1 glycosylation is poorly understood; however, the peptide sequence and n ...200919073881
functional characterization of a stilbene synthase gene using a transient expression system in planta.the expression and functionality of a resveratrol synthase (rs) gene from peanut (arachis hypogaea) was studied using an agrobacterium tumefaciens-mediated transient expression system in nicotiana benthamiana leaves. functional analysis of rs was demonstrated by tracking its expression during 96 h. to measure the transcripts levels of rs transgene, real-time qrt-pcr was used and revealed that the highest level of transcripts was at 48 h post-transfection. western blot analyses showed that rs pro ...200919116720
transformed hairy roots of arachis hypogea: a tool for studying root nodule symbiosis in a non-infection thread legume of the aeschynomeneae tribe.arachis hypogea is a non-"infection thread" (it) legume where rhizobial entry or dissemination in the nodules never involves it. rhizobia invade through epidermal "cracks" and directly access the cortical cells to develop the characteristic aeschynomenoid nodules. for investigating these nonclassical nodulation features in arachis spp., we developed an efficient procedure for agrobacterium rhizogenes r1000-mediated transformation of this plant. in this study, we optimized the induction of hairy ...200919132866
multilocus sequence analysis of the genus bradyrhizobium.the use of multilocus sequence analysis (mlsa) for the taxonomy of bradyrhizobium was assessed. we compared partial sequences for atpd, reca, gyrb, rpob and dnak for a set of reference strains representing named species and genospecies, and a number of new isolates from lupinus albus, arachis hypogaea and ornithopus compressus from spain. the phylogenies of the individual genes were compared with previous dna-dna hybridization results. high hybridization values were well reflected, but intermedi ...200919201125
a mitogen-activated protein kinase gene, ahmpk3 of peanut: molecular cloning, genomic organization, and heterologous expression conferring resistance against spodoptera litura in tobacco.mitogen-activated protein kinase cascade plays a very important role in plant signal transduction mechanism. a full length cdna of 1,514 bp length, corresponding to a mitogen-activated protein kinase gene was cloned from peanut (arachis hypogaea). based on its high homology with arabidopsis atmpk3, the cdna was designated as ahmpk3. it carried an open reading frame of 1,113 bp encoding a 371 amino acid polypeptide. ahmpk3 bears tey motif in its activation loop and belongs to the a1 subgroup of m ...200919352711
mucin-type o-glycans in tears of normal subjects and patients with non-sjögren's dry eye.o-linked carbohydrates (o-glycans) contribute to the hydrophilic character of mucins in mucosal tissues. this study was conducted to identify the repertoire of o-glycans in the tear film and the glycosyltransferases associated with their biosynthesis, in normal subjects and patients with non-sjögren's dry eye.200919407012
engineering of plant-specific phenylpropanoids biosynthesis in streptomyces venezuelae.phenylpropanoids, including flavonoids and stilbenes, are plant secondary metabolites with potential pharmacological and nutraceutical properties. to expand the applicability of streptomyces venezuelae as a heterologous host to plant polyketide production, flavonoid and stilbene biosynthetic genes were expressed in an engineered strain of s. venezuelae dhs2001 bearing a deletion of native pikromycin polyketide synthase gene. a plasmid expressing the 4-coumarate/cinnamate:coenzyme a ligase from s ...200919433224
l-methioninase production by aspergillus flavipes under solid-state fermentation.solid-state fermentation was carried out for the production of extra-cellular l-methioninase by aspergillus flavipes (bain and sart.) using nine agro-industrial residues, namely wheat bran, rice bran, wheat flour, coconut seeds, cotton seeds, ground nut cake, lentil hulls, soya beans and chicken feathers. chicken feathers were selected as solid substrate for l-methioninase production by a. flavipes. the maximum l-methioninase productivity (71.0 u/mg protein) and growth (11 mg protein/ml) of a. f ...200919455514
phenotypic and genotypic characterizations of rhizobia isolated from root nodules of peanut (arachis hypogaea l.) grown in moroccan soils.the phenotypic and genotypic characterization of sixty-two rhizobial isolates obtained from nodules of arachis hypogaea in north-western morocco was performed. their physiological and biochemical properties revealed a great deal of diversity among them. isolates were classified into two major groups based on the numerical analysis of their phenotypic and genotypic characteristics. isolates in the first group were alkali- and salt-sensitive, slow or extra-slow growers; they did not use disacchari ...200919455516
sialic acid in human testis and changes with aging.the aim of the present study was to investigate the distribution of the glycoconjugates sialoderivatives in the human testis. orchidectomy specimens from men aged 18-30 years (group 1) and from men aged 70-93 years (group 2) were obtained at autopsy. the study was performed using digoxigenin-labelled lectins, namely maackia amurensis ii lectin (maa), sambucus nigra agglutinin (sna) and arachis hypogaea lectin (pna), in addition to enzymatic and chemical treatments (neuraminidase, koh-neuraminida ...200919486598
intake, digestibility, and nitrogen retention by sheep supplemented with warm-season legume haylages or soybean meal.the high cost of commercial supplements necessitates evaluation of alternatives for ruminant livestock fed poor quality warm-season grasses. this study determined how supplementing bahiagrass haylage (paspalum notatum flügge cv. tifton 9) with soybean [glycine max (l.) merr.] meal or warm-season legume haylages affected the performance of lambs. forty-two dorper x katadhin lambs (27.5 +/- 5 kg) were fed for ad libitum intake of bahiagrass haylage (67.8% ndf, 9.6% cp) alone (control) or supplemen ...200919502512
intake, digestibility, and nitrogen retention by sheep supplemented with warm-season legume hays or soybean meal.the increasing cost of feed supplements necessitates evaluation of alternatives for ruminant livestock grazing poor quality warm-season grasses. this study determined how supplementing bahiagrass hay (paspalum notatum flügge cv. pensacola) with soybean [glycine max (l.) merr.] meal or warm-season legume hays affected intake, digestibility, and n utilization by lambs. dorper x katadhin crossbred lambs (30.6 +/- 5.5 kg; n = 42) were fed bahiagrass hay (73.8% ndf, 8.1% cp) for ad libitum intake and ...200919502513
cloning and sequence analysis of putative type ii fatty acid synthase genes from arachis hypogaea l.the cultivated peanut is a valuable source of dietary oil and ranks fifth among the world oil crops. plant fatty acid biosynthesis is catalysed by type ii fatty acid synthase (fas) in plastids and mitochondria. by constructing a full-length cdna library derived from immature peanut seeds and homology-based cloning, candidate genes of acyl carrier protein (acp), malonyl-coa:acp transacylase, beta-ketoacyl-acp synthase (i, ii, iii), beta-ketoacyl-acp reductase, beta-hydroxyacyl-acp dehydrase and e ...200919550039
improved methodology for the detection and quantification of the acrosome reaction in mouse spermatozoa.this study evaluates the use of two fluorescein-labelled (fitc) plant lectins, pisum sativum (edible pea) agglutinin (psa) and arachis hypogaea (peanut) agglutinin (pna), in order to determine the most accurate and reliable method to experimentally detect and assess the acrosome reaction in mouse spermatozoa. pna-fitc labelling was restricted to the acrosome and was not influenced by the fixation procedure; either absolute methanol or paraformaldehyde. in contrast, psa-fitc not only labelled the ...200919554507
spatiotemporal patterns and dispersal of stink bugs (heteroptera: pentatomidae) in peanut-cotton farmscapes.in the southeast united states, a field of peanuts, arachis hypogaea l., is often closely associated with a field of cotton, gossypium hirsutum l. the objective of this 4-yr on-farm study was to examine and compare the spatiotemporal patterns and dispersal of the southern green stink bug, nezara viridula l., and the brown stink bug, euschistus servus (say), in six of these peanut-cotton farmscapes. gs(+) version 9 was used to generate interpolated estimates of stink bug density by inverse distan ...200919689882
identification of candidate genome regions controlling disease resistance in arachis.worldwide, diseases are important reducers of peanut (arachis hypogaea) yield. sources of resistance against many diseases are available in cultivated peanut genotypes, although often not in farmer preferred varieties. wild species generally harbor greater levels of resistance and even apparent immunity, although the linkage of agronomically un-adapted wild alleles with wild disease resistance genes is inevitable. marker-assisted selection has the potential to facilitate the combination of both ...200919698131
crop juxtaposition affects cotton fiber quality in georgia farmscapes.phytophagous stink bugs (hemiptera: pentatomidae), including green stink bug [acrosternum hilare (say)], southern green stink bug [nezara viridula (l.)], and brown stink bug [euschistus servus (say)], have become a serious production issue for southeastern u.s. cotton, gossypium hirsutum l., growers. to investigate how different agronomic crops may affect stink bug damage and fiber quality in neighboring cotton fields, replicated 1.6-2.0-ha trials were planted with corn (zea mays l.), peanut (ar ...200919736764
the preputial gland of the japanese serow capricornis crispus: ultrastructure and lectin histochemistry.preputial glands of the japanese serow were found to consist of the modified sebaceous gland and apocrine gland in both sexes. in the modified sebaceous gland, cells transformed gradually toward the lumen from plentiful cytoplasm to a sponge-like network. lipid droplets were observed in mitochondria of differentiating cells. by lectin histochemistry, differentiating cells were stained with arachis hypogaea (pna), triticum vulgaris (wga) and canavalia ensiformis (con a) lectins, while secretory c ...20092728845
exposure of alpha2,6-sialylated lactosaminic chains marks apoptotic and necrotic death in different cell types.many observations have reported glycosylation changes associated with apoptosis in different biological systems, although none of these has shown any general significance. in this work, we show that in cell lines from different histological origin, (colon, breast, pancreas, and bladder cancer) as well as in normal human and mice neutrophils, apoptosis is accompanied by the exposure of sugar chains recognized by the lectin from sambucus nigra (sna), specific for sia alpha 2,6gal/galnac structures ...200918988689
mapping and conformational analysis of ige-binding epitopic regions on the molecular surface of the major ara h 3 legumin allergen of peanut (arachis hypogaea).eight distinct sequential ige-binding epitopes were identified along the amino acid sequence of ara h 3 using the spot technology. they essentially correspond to preferencially electropositive regions exposed on the molecular surface of the protein. a few ige-binding epitopes are coalescent to create more extended ige-binding regions exposed on the surface of the allergen. ara h 3 contains a core region corresponding to the cupin motifs and predicted to be preserved upon the trypsin and chymotry ...200918995911
studies of the cell surface properties of candida species and relation to the production of biosurfactants for environmental applications.in practical bioremediation of petroleum pollution, treatment systems often use soil, sand, and other aquifer porous media besides water solutions. the distribution of the microbial cell also plays an important role in the whole process of bioremediation; therefore, the adhesion ability of cells to porous media is one of the key factors influencing the efficiency of treatment. the probable modes of hydrocarbon uptake in cells of candida were studied based on data for cell hydrophobicity, emulsif ...200919005724
effects of treated sewage sludge levels on temporal variations of some soil properties of a typic xerofluvent soil in menemen plain, western anatolia, turkey.the aim of this study is to determine effects of treated sewage sludge (tss) levels as an organic matter (om) resource on temporal variations of some soil properties of a typic xerofluvent soil. the experiment was conducted in menemen plain, in the western anatolia region of turkey (latitudes 38 degrees 34'48.22''-38 degrees 34'49.24'' n; longitudes 27 degrees 1'23.05-27 degrees 1'24.14'' e) in the years of 2003 and 2004. moist tss was added to the soil at the rates of 0, 30, 60 and 90 t ha(-1) ...200918202900
effect of the new pyrazole carboxamide fungicide penthiopyrad on late leaf spot and stem rot of peanut.management of early leaf spot (cercospora arachidicola hori.), late leaf spot [cercosporidium personatum (berk. & ma curtis) deighton] and stem rot (sclerotium rolfsii sacc.) of peanut (arachis hypogaea l.) in the southeastern usa is heavily dependent upon sterol biosynthesis inhibitor (sbi) and quinone outside inhibitor (qoi) fungicides. effective new fungicides with different modes of action could improve overall disease control and extend the utility of the current fungicides. penthiopryad is ...200918785218
influence of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina.three studies were conducted to determine the effect of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina. in all three studies, samples of wireworm populations were taken from the soil by using oat, avena sativa l., baits. treatments were defined by the previous year's crop and were chosen to reflect common crop rotations in the region. across all three studies, eight wireworm species were recovered from the baits: conoderus amplicollis (gyllen ...201021309229
vip3aa tolerance response of helicoverpa armigera populations from a cry1ac cotton planting region.transgenic cotton, gossypium hirsutum l., that expresses the bacillus thuringiensis (bt) cry1ac toxin, holds great promise in controlling target insect pests. evolution of resistance by target pests is the primary threat to the continued efficacy of bt cotton. to thwart pest resistance evolution, a transgenic cotton culitvar that produces two different bt toxins, cry1ac and vip3a genes, was proposed as a successor of cry1ac cotton. this article reports on levels of vip3aa tolerance in helicoverp ...201021309241
characterization of the biosynthesis and cell surface expression of avian metapneumovirus attachment glycoprotein.biosynthesis, glycosylation and cell surface expression of the ampv/c g protein were examined in eukaryotic cell lines (llc-mk2, cho-k1, cho-1d1d). immature g gene products with a molecular mass of 42, 45 and 58-90 kilodaltons (kda) were identified by sds-page and represented glycosylated intermediates. tunicamycin treatment of transfected cells confirmed the presence of n-linked carbohydrate moieties on these intermediate species and identified a 38 kda unglycosylated precursor. a fully process ...201019896993
immediate and delayed (after cooling) effects of centrifugation on equine sperm.the objectives of this study were to determine the effects of centrifugation on equine sperm total and progressive motility, viability, and acrosomal integrity. we hypothesized that although high centrifugation forces would be detrimental to equine equus caballus sperm, recovery rates would increase. ejaculates from six stallions were collected, extended to a concentration of 25x10(6) cells/ml, and subjected for 10min to (1) no centrifugation (nc) or (2) centrifugation at 400xg, (3) 900xg, or (4 ...201019913898
new dimeric stilbenoids from fungal-challenged peanut ( arachis hypogaea) seeds.the peanut plant can resist fungal attacks by producing stilbene-derived phytoalexins. once understood, such a natural phytoalexin-based mechanism of peanut resistance could be potentially manipulated to obtain fungal-resistant peanut breeding lines. several simple stilbenoid phytoalexins from peanuts have been reported. however, more complex stilbenoid derivatives such as those that have been reported from other sources and considered important factors in plant defense have not been found in pe ...201020020707
phosphatidylcholine levels of peanut-nodulating bradyrhizobium sp. semia 6144 affect cell size and motility.phosphatidylcholine, the major phospholipid in eukaryotes, is found in rhizobia and in many other bacteria interacting with eukaryotic hosts. phosphatidylcholine has been shown to be required for a successful interaction of bradyrhizobium japonicum usda 110 with soybean roots. our aim was to study the role of bacterial phosphatidylcholine in the bradyrhizobium-peanut (arachis hypogaea) symbiosis. phospholipid n-methyltransferase (pmt) and minor phosphatidylcholine synthase (pcs) activities were ...201020030724
a model of the acrosome reaction progression via the acrosomal membrane-anchored protein equatorin.it is important to establish a reliable and progressive model of the acrosome reaction. here, we present a progression model of the acrosome reaction centering around the acrosomal membrane-anchored protein equatorin (mn9), comparing the staining pattern traced by mn9 antibody immunofluorescence with that traced by arachis hypogaea agglutinin (pna)-fitc. prior to the acrosome reaction, equatorin was present in both the anterior acrosome and the equatorial segment. since sperm on zona pellucida s ...201020032212
some nutritional characteristics of kernel and oil of peanut (arachis hypogaea l.).some nutritional properties of the peanut kernel and oils were established. the oil yields from these kernels vary from 32.7 % to 45.4 %. the content of protein ranged between 25.9 % to 32.4 %, with a mean value of 28.93 %. the mineral contents of peanut kernels were determined by inductively coupled plasma atomic emission spectrometry (icp-aes). the contents of na ranged from 867.7 mg/kg to 1186.1 mg/kg, with a mean value of 1004.7 mg/ kg. the phosphor contents of kernels ranged between 2769.7 ...201020032593
survey of glycoantigens in cells from alpha1-3galactosyltransferase knockout pig using a lectin microarray.glycoantigens represent major obstacles to successful xenotransplantation. even after the alpha1-3galactosyltransferase (galt) gene knockout (galt-ko) pigs were produced, non-gal antigens continue to be present. this study reports on lectin blot analyses for endothelial cells (ec) and fibroblasts from galt-ko pigs.201020149189
lectin-binding sites on ejaculated stallion sperm during breeding and non-breeding periods.stallion sperm from semen collected in southern italy during the breeding (june-july) and non-breeding (december-january) periods were analyzed by means of twelve lectins to evaluate the glycoconjugate pattern and to verify whether there are any seasonal differences in the glycosylation pattern of the sperm glycocalyx. the acrosomal cap showed reactivity for maackia amurensis (mal ii), sambucus nigra (sna), arachis hypogaea (pna), glycine max (sba), helix pomatia (hpa), canavalia ensiformis (con ...201020172596
an efficient method for the production of marker-free transgenic plants of peanut (arachis hypogaea l.).recombinant genes conferring resistance to antibiotics or herbicides are widely used as selectable markers in plant transformation for selecting the primary transgenic events. however, these become redundant once the transgenic plants have been developed and identified. although, there is no evidence that the selectable marker genes are unsafe for consumers and the environment, it would be desirable if the marker genes can be eliminated from the final transgenic events. the availability of effic ...201020217416
a mitogen-activated protein kinase, ahmpk6 from peanut localizes to the nucleus and also induces defense responses upon transient expression in tobacco.mitogen-activated protein kinase (mapk) cascades are one of the well studied signaling networks in plants. in this study a full length cdna from arachis hypogaea (peanut) encoding a mitogen-activated protein kinase was cloned using race-pcr approach. the cdna was designated as ahmpk6 based on its high identity with atmpk6 of arabidopsis. the ahmpk6 encodes a 397 amino acid polypeptide that shows high similarity with mssimk, ntsipk, ossipk of medicago, tobacco and rice respectively. phylogenetic ...201020409725
magnetic bead-based fluorometric detection of lectin-glycoprotein interactions.a sandwich-type bioassay was developed to detect the lectin-glycoprotein interactions using fluorescein isothiocyanate (fitc) as the label. the bioassay was optimized using the well-described lectins and their target glycoprotein. the biotinylated lectins from triticum vulgaris (wheat germ, wga) and arachis hypogaea (peanut, pna) were immobilized on streptavidin-coated superparamagnetic iron oxide microparticles (beads) and utilized for the isolation of target biomolecules (invertase from saccha ...201020441957
identification of the glycosylation pattern of meat proteins in tissues from bovine myelin.a variant form of creutzfeld-jacob disease in humans is associated with the consumption of food contaminated with the bovine central neural system. this has focused attention on the need for procedures to detect tissues of the neural system in meat and meat products. a method was developed for the identification of myelin glycoproteins in bovine neural tissue. the glycosylated structures of glycoproteins in different protein mixtures from central nervous system (cns) and peripheral nervous syste ...201020513571
a qtl study on late leaf spot and rust revealed one major qtl for molecular breeding for rust resistance in groundnut (arachis hypogaea l.).late leaf spot (lls) and rust are two major foliar diseases of groundnut (arachis hypogaea l.) that often occur together leading to 50-70% yield loss in the crop. a total of 268 recombinant inbred lines of a mapping population tag 24 x gpbd 4 segregating for lls and rust were used to undertake quantitative trait locus (qtl) analysis. phenotyping of the population was carried out under artificial disease epiphytotics. positive correlations between different stages, high to very high heritability ...201020526757
electrochemical immunosensors for antibodies to peanut allergen ara h2 using gold nanoparticle-peptide films.life-threatening allergies to peanuts and tree nuts can be revealed by detecting antibodies (iges) to their allergens in patient serum. herein, we compare several immunosensor-like methodologies for sensitive detection of antibodies to a peptide sequence from the major peanut allergen, arachis hypogaea 2 (ara h2). the sensors feature a synthetic peptide layer of the major ige-binding epitope from ara h2 attached to a dense gold nanoparticle (aunp) film on a pyrolytic graphite (pg) electrode. the ...201020540504
first trimester histiotrophe shows altered sialylation compared with secretory phase glycoconjugates in human endometrium.histiotrophe is now recognized as being an important feature of early human pregnancy, providing nutrients and growth factors to the developing embryo. our aim was to examine the glycan composition of histiotrophe from first trimester decidua and to compare it with secretions present in endometrial glands from the late secretory phase of the menstrual cycle.201020569980
aging and food source effects on mandibular stylets teeth wear of phytophagous stink bug (heteroptera: pentatomidae).studies were conducted to test the effect of age and food sources on wear of the mandibular teeth of the phytophagous pentatomid, euschistus heros (f.). the total length (µm) of the area bearing the mandibular teeth, the length of the 1st tooth, and the height of the 2nd tooth for teneral (< 1 day-old) adults were significantly greater than that of adults fed on natural [green bean, phaseolus vulgaris pods, raw shelled peanuts, arachis hypogaea, and mature soybean, glycine max seeds] for 30 or 6 ...201021271064
antimicrobial and anti-adhesive potential of a biosurfactant rufisan produced by candida lipolytica ucp 0988.in the last years, researches developed with biosurfactants for application in the medical area have been revealing the promising biological activities of these biomolecules. in this work the antimicrobial and anti-adhesive properties of a biosurfactant rufisan isolated from the yeast candida lipolytica ucp 0988, growth in a medium supplemented with ground nut refinery residue was determined against several microorganisms. the biosurfactant was able to reduce the water surface tension from 70 to ...201021247740
pterocarpenes elicited by aspergillus caelatus in peanut (arachis hypogaea) seeds.the substituted pterocarpenes named aracarpene-1 (1) and aracarpene-2 (2) were isolated from wounded peanut seeds challenged by a strain of aspergillus caelatus. the structures of these putative phytoalexins were determined by interpretation of nmr and ms data. the aracarpenes were investigated for their antifungal and antibacterial activities as well as antioxidant, anti-inflammatory, and cytotoxic activities in mammalian cells. aracarpene-2 demonstrated high antibacterial properties against te ...201021030054
bradyrhizobium lablabi sp. nov., isolated from effective nodules of lablab purpureus and arachis hypogaea grown in southern china.five strains isolated from root nodules of lablab purpureus and arachis hypogaea grown in anhui and sichuan provinces of china were classified within the genus bradyrhizobium. these strains had identical 16s rrna nucleotide sequences which shared 99.22% and 99.48% similarities with the most related strains of bradyrhizobium jicamae pac 68(t), bradyrhizobium pachyrhizi pac 48(t) and bradyrhizobium elkanii usda 76(t). polyphasic approaches including 16s rrna gene rflp, igs-rflp, box-pcr, comparati ...201021112989
identification of seed proteins associated with resistance to pre-harvested aflatoxin contamination in peanut (arachis hypogaea l).pre-harvest infection of peanuts by aspergillus flavus and subsequent aflatoxin contamination is one of the food safety factors that most severely impair peanut productivity and human and animal health, especially in arid and semi-arid tropical areas. some peanut cultivars with natural pre-harvest resistance to aflatoxin contamination have been identified through field screening. however, little is known about the resistance mechanism, which has slowed the incorporation of resistance into cultiv ...201021118527
release of soluble protein from peanut (arachis hypogaea, leguminosae) and its adsorption by activated charcoal.peanut (arachis hypogaea, leguminosae) allergy is a major cause of food-induced anaphylaxis. the potential use of activated charcoal (ac) to adsorb and reduce the bioavailability of peanut protein allergens for use in the moderation of hypersensitivity reactions was investigated. the rate and extent of protein release from peanut and the adsorption of the solubilized protein by ac was determined under physiological ph values and confirmed in vivo using a porcine animal model system. peanut prote ...201021128612
immune responses to hemagglutinin-neuraminidase protein of peste des petits ruminants virus expressed in transgenic peanut plants in sheep.peste des petits ruminants (ppr) is an acute, highly contagious disease of small ruminants caused by a morbillivirus, peste des petits ruminants virus (pprv). the disease is prevalent in equatorial africa, the middle east, and the indian subcontinent. a live attenuated vaccine is in use in some of the countries and has been shown to provide protection for at least three years against ppr. however, the live attenuated vaccine is not robust in terms of thermotolerance. as a step towards developmen ...201021211855
evaluation of fresh and frozen-thawed fowl semen by flow cytometry.the aim of this study was to assess the spermatozoal viability, acrosome integrity, mitochondrial activity, and dna status in the frozen-thawed fowl semen with the use of flow cytometry. the experiment was carried out on 10 sexually adult roosters of meat type line flex. the semen was collected three times a week by dorso-abdominal massage method, then pooled and subjected to cryopreservation using "pellet" method and dimethylacetamide (dma) as a cryoprotectant. for cytometric analysis the fresh ...201020580066
purification of resveratrol, arachidin-1, and arachidin-3 from hairy root cultures of peanut (arachis hypogaea) and determination of their antioxidant activity and cytotoxicity.antioxidant stilbenoids, such as resveratrol, arachidin-1, and arachidin-3, have demonstrated beneficial effects on human health. although resveratrol is commercially available, arachidin-1 and arachidin-3 are not, resulting in an opportunity to explore purification methods and to confirm biological activity. recently, arachis hypogaea hairy root cultures (produced via agrobacterium rhizogenes-mediated transformation) were reported to secrete stilbenoids into liquid growth media upon elicitation ...201020623593
analysis of expression and glycosylation of avian metapneumovirus attachment glycoprotein from recombinant baculoviruses.recently, we reported the expression and glycosylation of avian metapneumovirus attachment glycoprotein (ampv/c g protein) in eukaryotic cell lines by a transient-expression method. in the present study, we investigated the biosynthesis and o-linked glycosylation of the ampv/c g protein in a baculovirus expression system. the results showed that the insect cell-produced g protein migrated more rapidly in sds-page as compared to llc-mk2 cell-derived g proteins owing to glycosylation differences. ...201020713098
comparing symbiotic efficiency between swollen versus nonswollen rhizobial bacteroids.symbiotic rhizobia differentiate physiologically and morphologically into nitrogen-fixing bacteroids inside legume host nodules. the differentiation is apparently terminal in some legume species, such as peas (pisum sativum) and peanuts (arachis hypogaea), likely due to extreme cell swelling induced by the host. in other legume species, such as beans (phaseolus vulgaris) and cowpeas (vigna unguiculata), differentiation into bacteroids, which are similar in size and shape to free-living rhizobia, ...201020837702
potential of trap crops for integrated management of the tropical armyworm, spodoptera litura in tobacco.the tropical armyworm, spodoptera litura (f.) (lepidoptera: noctuidae), is an important pest of tobacco, nicotiana tabacum l. (solanales: solanaceae), in south china that is becoming increasingly resistant to pesticides. six potential trap crops were evaluated to control s. litura on tobacco. castor bean, ricinus communis l. (malpighiales: euphorbiaceae), and taro, colocasia esculenta (l.) schott (alismatales: araceae), hosted significantly more s. litura than peanut, arachis hypogaea l. (fabale ...201020874598
epigenetic regulation of peanut allergen gene ara h 3 in developing embryos.peanut (arachis hypogaea) allergy is one of the most serious food allergies. peanut seed protein, ara h 3, is considered to be one of the most important peanut allergens. little is known about the temporal and spatial regulation mechanism of ara h 3 during seed development. in this study, chromatin structure of the ara h 3 promoter was analyzed to examine its transcriptional regulation. analysis of transgenic plants of arabidopsis thaliana expressing arah3: gus showed that the ara h 3 promoter c ...201020157727
deep sequencing identifies novel and conserved micrornas in peanuts (arachis hypogaea l.).micrornas (mirnas) are a new class of small, endogenous rnas that play a regulatory role in the cell by negatively affecting gene expression at the post-transcriptional level. mirnas have been shown to control numerous genes involved in various biological and metabolic processes. there have been extensive studies on discovering mirnas and analyzing their functions in model species, such as arabidopsis and rice. increasing investigations have been performed on important agricultural crops includi ...201020047695
phylogenetic analyses of peanut resistance gene candidates and screening of different genotypes for polymorphic markers.the nucleotide-binding-site-leucine-rich-repeat (nbs-lrr)-encoding gene family has attracted much research interest because approximately 75% of the plant disease resistance genes that have been cloned to date are from this gene family. here, we describe a collection of peanut nbs-lrr resistance gene candidates (rgcs) isolated from peanut (arachis) species by mining gene bank data base. nbs-lrr sequences assembled into tir-nbs-lrr (75.4%) and non-tir-nbs-lrr (24.6%) subfamilies. total of 20 dist ...201023961057
antioxidant activities of curcumin and combinations of this curcuminoid with other phytochemicals.biomedical investigations of curcumin (and curcuminoids) have provided evidence of a wide range of molecular and cellular activities, most related to redox reactions and signal transduction. the main goal of the present study was to compare antioxidant activities of curcumin with those of resveratrol, a polyphenol present in some dietary plants such as vitis vinifera (l.) and arachis hypogaea (l.) and many other, non-dietary plants. combinations of the two were also examined for potential synerg ...201019927272
fidel-a retrovirus-like retrotransposon and its distinct evolutionary histories in the a- and b-genome components of cultivated peanut.in this paper, we describe a ty3-gypsy retrotransposon from allotetraploid peanut (arachis hypogaea) and its putative diploid ancestors arachis duranensis (a-genome) and arachis ipaënsis (b-genome). the consensus sequence is 11,223 bp. the element, named fidel (fairly long inter-dispersed euchromatic ltr retrotransposon), is more frequent in the a- than in the b-genome, with copy numbers of about 3,000 (+/-950, a. duranensis), 820 (+/-480, a. ipaënsis), and 3,900 (+/-1,500, a. hypogaea) per hapl ...201020127167
a ssr-based composite genetic linkage map for the cultivated peanut (arachis hypogaea l.) genome.the construction of genetic linkage maps for cultivated peanut (arachis hypogaea l.) has and continues to be an important research goal to facilitate quantitative trait locus (qtl) analysis and gene tagging for use in a marker-assisted selection in breeding. even though a few maps have been developed, they were constructed using diploid or interspecific tetraploid populations. the most recently published intra-specific map was constructed from the cross of cultivated peanuts, in which only 135 s ...201020105299
genetic relationships among seven sections of genus arachis studied by using ssr markers.the genus arachis, originated in south america, is divided into nine taxonomical sections comprising of 80 species. most of the arachis species are diploids (2n = 2x = 20) and the tetraploid species (2n = 2x = 40) are found in sections arachis, extranervosae and rhizomatosae. diploid species have great potential to be used as resistance sources for agronomic traits like pests and diseases, drought related traits and different life cycle spans. understanding of genetic relationships among wild sp ...201020089171
quality index of oils extracted from gamma-irradiated peanuts (arachis hypogaea l.) of the golden and bari varieties.two varieties of peanuts were irradiated to 4, 6 and 8 kgy with co(60). their proximate compositions remained unaffected, but microbes were eliminated completely after irradiation to 8 kgy. hplc was used to study tocopherols of irradiated and unirradiated oil samples. there were dose-dependent differences in physico-chemical values between the control and irradiated samples. significant changes in tocopherol concentrations and peroxide values in the oils were observed after irradiation to 8 kgy. ...201020615718
species relationships among the wild b genome of arachis species (section arachis) based on fish mapping of rdna loci and heterochromatin detection: a new proposal for genome arrangement.arachis hypogaea is an allotetraploid species with low genetic variability. its closest relatives, all of the genus arachis, are important sources of alleles for peanut breeding. however, a better understanding of the genome constitution of the species and of the relationships among taxa is needed for the effective use of the secondary gene pool of arachis. in the present work, we focused on all 11 non-a genome (or b genome sensu lato) species of arachis recognized so far. detailed karyotypes we ...201020552326
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