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effects of ultraviolet-b irradiation on the cuticular wax of cucumber (cucumis sativus) cotyledons.cucumber seedlings were grown under three doses of supplemental ultraviolet-b (uv-b) irradiation to examine the effects on the surface structure of the cotyledons. medium and high doses of irradiation induced glazing (formation of translucent, glossy layers) on the adaxial surfaces of cotyledons, especially those exposed to a high dose of uv-b. observation with a scanning electron microscope revealed that the adaxial surfaces of cotyledons exposed to a medium dose of uv-b and controls became rou ...200818217194
exogenous spermidine differentially alters activities of some scavenging system enzymes, h(2)o(2) and superoxide radical levels in water-stressed cucumber leaves.in order to examine whether polyamines (pas) modify the functioning of the scavenging system and oxidative stress levels in water-stressed plants, cucumber (cucumis sativus l.) seedlings were treated with spermidine (spd) prior to dehydration, and stress-evoked changes in superoxide dismutase (sod) (ec 1.15.1.1), catalase (ec 1.11.1.6), guaiacol peroxidase (ec 1.11.1.7) activities, h(2)o(2) and superoxide radical levels were determined. free pa content during spd treatment and during the stress ...200817658660
comparison of heavy metal effect on the proton pumps of plasma membrane and tonoplast in cucumber root cells.the effects of 10 microm cadmium, copper and nickel on the activities of vacuolar membrane and plasma membrane (pm) atp-dependent proton pumps was investigated in cucumis sativus l. root cells. it was demonstrated that vacuolar h+-atpase (ec 3.6.3.14) and pm h+-atpase (ec 3.6.3.6) differed in sensitivity to heavy metals. exposure of cucumber seedlings to cd, cu and ni had no significant effect on the activity of the vacuolar proton pump and, in the case of ni, also on the activity of the pm prot ...200817658657
protein oxidation in the leaves and roots of cucumber plants (cucumis sativus l.), mutant msc16 and wild type.reactive oxygen species (ros) may cause irreversible carbonylation of proteins, resulting in structural and/or functional modifications. carbonylated proteins were analyzed and compared in tissue extracts or purified mitochondria isolated from the leaves and roots of wild-type (wt) or msc16 mutant cucumber plants. for analysis of the oxidized protein formation and degradation, several techniques were applied: western blotting, quantitative, spectrophotometric assay of carbonyl concentration and ...200817928097
pesticide residues in agricultural products monitored in hyogo prefecture, japan, fys 1995-1999.during a 5-year monitoring survey (april 1995-march 2000) of pesticide residues in agricultural products, 765 samples (478 domestic; 287 imported) collected in hyogo prefecture, japan, were analyzed. the number of pesticides tested increased from 107 in fiscal year (fy) 1995 to 204 in fy 1999. the purpose of the study was to promote consumer safety by excluding the food illegally containing pesticide residues from markets. overall, 51 % of domestic and 32% of imported samples contained no detect ...200812083262
multiresidue liquid chromatographic method for simultaneous determination of pyrethroid insecticides in fruits and vegetables.a simple and rapid liquid chromatographic (lc) method has been developed for simultaneous determination of 9 pyrethroid insecticides (biphenthrin, cypermethrin, fenpropathrin, fenvalerate, flucythrinate, methothrin, permethrin, py-115, and tetramethrin) in fruits and vegetables. residues are extracted from crops with methanol and partitioned with toluene. extracts are cleaned up by florisil-charcoal column chromatography. lc separation is performed on a mubondapak c18 stainless steel column with ...20088664585
[signal trait of cucumbers].a mutant of cucumber characterized by the absence of nappiness on all the organs of the plant was found at birjuchekutian station in 1970. this character is recessive and is manifested in the second generation showing a 3:1 ratio of nappy and plain plants. on the basis of this mutant, forms of cucumber without nappiness were obtained. plain plants are distinguished especially well at the initial stages of development when the first leaf appears. they are viable and productive, grow perfectly wel ...2008929676
evidence for nucleosomal phasing and a novel protein specifically binding to cucumber satellite dna.the nucleosomal organization and the protein-binding capability of highly repeated and methylated satellite dna of cucumber (cucumis sativus l.), comprising approx. 30% of the genome, were analyzed. nucleosomal core dna from satellite type i was prepared after micrococcal nuclease digestion of chromatin and sequenced. most of the core sequences obtained could be grouped in two main (a and b) and two minor groups (c and d) indicating a specific and complex phasing of nucleosomes on this satellite ...20098148012
differential changes in the steady state levels of thylakoid membrane proteins during senescence in cucumis sativus cotyledons.chloroplast structure and function is known to alter during foliar senescence. besides, the alterations in the structural organisation of thylakoid membranes changes in the steady state levels of thylakoid membrane proteins occur due to leaf ageing. we monitored temporal changes in some of the specific proteins of thylakoid membrane protein complexes by western blotting in the cucumis sativus cotyledons as a function of the cotyledon age. we observed that the levels of d1 and d2 proteins of phot ...200911531094
characterization of cssef1 gene encoding putative ccch-type zinc finger protein expressed during cucumber somatic embryogenesis.somatic embryos obtained in vitro are a form of vegetative reproduction that can be used in artificial seed technology, as well as a model to study the principles of plant development. in order to isolate the genes involved in somatic embryogenesis of the cucumber (cucumis sativus l.), we utilized the suppression subtractive hybridization (ssh). one of the obtained sequences was the cssef1 clone (cucumis sativus somatic embryogenesis zinc finger 1), with a level of expression that sharply increa ...200918778873
toxicity of perfluorooctane sulfonate and perfluorooctanoic acid to plants and aquatic invertebrates.acute toxicities of perfluorooctane sulfonate (pfos) and perfluorooctanoic acid (pfoa) were tested on four freshwater species and three plant species. pfos was more toxic than pfoa for all species tested in this study. similar time-response patterns of pfos and pfoa toxicity were observed for each tested species. values of the 48-h lc(50) of pfos for all test species ranged from 27 to 233 mg/l and values of the 96-h lc(50) for three of the species ranged from 10 to 178 mg/l. values of the 48-h l ...200918461560
effects of morphological characteristics of cucumis sativus seedlings grown at different vapor pressure deficits on initial colonization of bemisia tabaci (hemiptera: aleyrodidae).we investigated the effects of morphological characteristics of cucumber, cucumis sativus l., seedlings grown at different vapor pressure deficits (vpds) on initial colonization of whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) biotype b. the seedlings were grown at a vpd of 3.8 or 0.4 kpa for 9 d at 30 degrees c under a photoperiod of 12:12 (l:d) h. pairs of seedlings, one grown at high vpd and the other at low vpd, were then installed in cages with a vpd of 1.3 kpa, and adults of ...200920069856
effects of foliar surfactants on host plant selection behavior of liriomyza huidobrensis (diptera: agromyzidae).the pea leafminer, liriomyza huidobrensis (diptera: agromyzidae), is a highly polyphagous insect pest of global distribution. l. huidobrensis feeds and lays its eggs on leaf tissue and reduces crop marketability because of stippling and mining damage. in field insecticide trials, it was observed that stippling was reduced on plants treated with surfactant alone. the objectives of this study were to determine the effect of surfactants on host selection behaviors of female l. huidobrensis and to a ...200919825293
differentiation of dried sea cucumber products from different geographical areas by surface desorption atmospheric pressure chemical ionization mass spectrometry.without any sample pretreatment, mass spectral fingerprints of 486 dried sea cucumber slices were rapidly recorded in the mass range of m/z 50-800 by using surface desorption atmospheric pressure chemical ionization mass spectrometry (dapci-ms). a set of 162 individual sea cucumbers (apostichopus japonicus selenka) grown up in 3 different geographical regions (weihai: 59 individuals, 177 slices; yantai: 53 individuals, 159 slices; dalian: 50 individuals, 150 slices;) in north china sea were succ ...200919788186
ngfffamide and echinotocin: structurally unrelated myoactive neuropeptides derived from neurophysin-containing precursors in sea urchins.the myoactive neuropeptide ngiwyamide was originally isolated from the holothurian (sea cucumber) apostichopus japonicus but there is evidence that ngiwyamide-like peptides also occur in other echinoderms. here we report the discovery of a gene in the sea urchin strongylocentrotus purpuratus that encodes two copies of an ngiwyamide-like peptide: asn-gly-phe-phe-phe-(nh(2)) or ngfffamide. interestingly, the c-terminal region of the ngfffamide precursor shares sequence similarity with neurophysins ...200919329739
neuronal peptides induce oocyte maturation and gamete spawning of sea cucumber, apostichopus japonicus.extracts prepared from tissues containing buccal ring nerve or longitudinal radial nerve of sea cucumber induce oocyte maturation and ovulation from ovarian tissues. we purified two small peptides, a pentapeptide and a heptapeptide, from the buccal tissues of japanese common sea cucumber, apostichopus japonicas. both peptides induced oocyte maturation and gamete spawning. the pentapeptide was identified as ngiwyamide. this peptide induced in vitro germinal vesicle breakdown and ovulation of full ...200919059232
lps-induced genes in intestinal tissue of the sea cucumber holothuria glaberrima.metazoan immunity is mainly associated with specialized cells that are directly involved with the immune response. nevertheless, both in vertebrates and invertebrates other organs might respond to immune activation and participate either directly or indirectly in the ongoing immune process. however, most of what is known about invertebrate immunity has been restricted to immune effector cells and little information is available on the immune responses of other tissues or organs. we now focus on ...200919584914
cytotoxic action of triterpene glycosides from sea cucumbers from the genus cucumaria on mouse spleen lymphocytes. inhibition of nonspecific esterase.four triterpene glycosides from sea cucumbers belonging to the genus cucumaria, okhotoside a(1)-1 (1), cucumarioside a(0)-1 (2), frondoside a (3) and cucumarioside a(2)-2 (4) inhibit the activity of nonspecific esterase of mouse spleen lymphocytes. the dependence of the inhibitory activity of the glycosides on their structure is similar to that for hemolytic activity. the absence of inhibitory activity for the preparation cumaside, which is a complex of cucumarioside a(2)-2 and related compounds ...200919634320
[etiology of off-plate syndrome in cultured larval apostichopus japonicus at attachment stage and analysis of reservoir of pathogens].to clarify pathogens and sources of the off-plate syndrome at the attachment stage in the larval culture of apostichopus japonicus, and further to find out effective medicines for this disease.200919637571
brassinosteroids promote metabolism of pesticides in cucumber.brassinosteroids (brs) are known to protect crops from the toxicity of herbicides, fungicides and insecticides. it is shown here that application of 24-epibrassinolide (ebr) accelerated metabolism of various pesticides and consequently reduced their residual levels in cucumber ( cucumis sativus l). chlorpyrifos, a widely used insecticide, caused significant reductions of net photosynthetic rate (pn) and quantum yield of psii (phi(psii)) in cucumber leaves. ebr pretreatment alleviated the decline ...200919694443
enhancement of non-specific immune response in sea cucumber (apostichopus japonicus) by astragalus membranaceus and its polysaccharides.in this study, the immunostimulatory effect of oral administration of different preparations (conventional fine powder [cp] and superfine powder [sp]) of astragalus membranaceus root or its polysaccharides (aps) in sea cucumber (apostichopus japonicus) was investigated. sea cucumbers with an average initial weight of 49.3 +/- 5.65 g were fed with a diet containing 3% cp or sp or 0.3% aps over a period of 60 days. the non-specific humoral (phenoloxidase, lysozyme and agglutination titer) and cell ...200919748588
lindane residues in cultivated cucumber and in the most consumed fish in caspian sea (iran).in this study, the concentrations of lindane residues (organochlorine pesticides) were analyzed in samples of cultivated cucumbers (cucumis sativus l.) and four species of most consumed fish (sefid, koli, kilca and kafal fish). samples of cucumber were collected from five sites in sari city (north, south, east, west and central areas) and samples of fish were caught using electric fishing from four major fishing centers (chalous and babolsar cities, khazar abad and miankaleh regions) in mazandar ...200919825858
risk assessment of cryptosporidium and giardia in water irrigating fresh produce in mexico.a rise in foodborne outbreaks in the united states associated with fresh produce has resulted in increased concerns with the importation of fruits and vegetables. mexico is a major exporter of produce to the united states, particularly tomatoes and bell peppers. a quantitative microbial risk assessment (qmra) was conducted to evaluate the public health impact of protozoan-laden water irrigating produce in culiacan, sinaloa, mexico. specifically, a qmra was conducted to address the human health i ...200919833043
modification of azo dyes by lactic acid bacteria.the ability of lactobacillus casei and lactobacillus paracasei to modify the azo dye, tartrazine, was recently documented as the result of the investigation on red coloured spoilage in acidified cucumbers. fourteen other lactic acid bacteria (lab) were screened for their capability to modify the food colouring tartrazine and other azo dyes of relevance for the textile industry.200919302317
[studies on the purification of water-soluble holothurian glycosides from apostichopus japonicus and their tumor suppressing activity].to obtain water-soluble holothurian glycosides with high tumor suppressing activities from apostichopus japonicus, macroporous resin, silica gel and gel-filtration column chromatograghy were used to purify the water-soluble holothurian glycosides, and their tumor suppressing activity and inducing apoptosis of tumor cells were examined in this study. the 70% ethanol fraction of macroporous resin column, the psc-2 and psc-3 fractions from silica gel column showed very strong tumor suppressing acti ...200919350817
reactive oxygen species are involved in brassinosteroid-induced stress tolerance in cucumber.brassinosteroids (brs) induce plant tolerance to a wide spectrum of stresses. to study how br induces stress tolerance, we manipulated the br levels in cucumber (cucumis sativus) through a chemical genetics approach and found that br levels were positively correlated with the tolerance to photo-oxidative and cold stresses and resistance to cucumber mosaic virus. we also showed that br treatment enhanced nadph oxidase activity and elevated h(2)o(2) levels in apoplast. h(2)o(2) levels were elevate ...200919386805
[cucumber diseases diagnosis using multispectral imaging technique].for a reliable diagnosis of plant diseases and insect pests, spectroscopy analysis technique and mutispectral imaging technique are proposed to diagnose five cucumber diseases, namely trichothecium roseum, sphaerotheca fuliginea, cladosporium cucumerinum, corynespora cassiicola and pseudoperonospora cubensis. in the experiment, the cucumbers' multispectral images of 14 visible lights channels, near infrared channel and panchromatic channel were captured using narrow-band multispectral imaging sy ...200919445229
[analysis study of trace elements in abalone and sea cucumber].abalone and sea cucumbers are useful, which is related with trace elements in them. in the present paper, using high-pressure sealed nitrifying pots for digestion of samples, orthogonal test was applied to the determination of 15 kinds of trace elements in abalone and sea cucumber such as fe, ca, mg, zn, sr, se, hg, cd etc by icp-aes. the orthogonal table of l16 (4(4)) and experiment programs were designed. more information could be obtained with a small number of experimental times by combining ...200919445240
characterization of an i-type lysozyme gene from the sea cucumber stichopus japonicus, and enzymatic and nonenzymatic antimicrobial activities of its recombinant protein.because sea cucumbers lack a well-developed immune system and can ingest pathogenic bacteria together with food, some form of active antibacterial substances must be present in the body for defense. in this study, the cdna of an i-type lysozyme from the sea cucumber stichopus japonicus (designated sjlys) was cloned by rt-pcr and race pcr techniques. the full length cdna of sjlys was 713 bp with an open reading frame of 438 bp coding for 145 amino acids. two catalytic residues (glu34 and asp47), ...200919447331
[recombinant expression and antibacterial activity of i-type lysozyme from sea cucumber stichopus japonicus].the cdna of an i type lysozyme was cloned from stichopus japonicus (named as sjlys). the dna fragment of the mature sjlys was subcloned into expression vector of pet-32a (+) to construct the recombinant plasmid of pet32a (+)-sjlys. the recombinant plasmid was then transformed into escherichia coli bl21 (de3) plyss and induced by isopropylthio-beta-d-galactoside (iptg). the recombinant protein expressed as inclusion bodies was denatured, partially purified and refolded to be an active form. the b ...200919459322
influence of microbial growth on the redox potential of fermented cucumbers.commonly, ph measurements are used during the production of fermented cucumbers to indirectly monitor growth of lactic acid bacteria (lab) and acid production. redox potential (e(h)) measurements, which are determined by the potential of an electron to reduce an acceptor, could serve as an alternative tool to monitor the progress of fermentation allowing the detection of the metabolic activity and/or growth of lab and other microorganisms. pasteurized and inoculated jars of cucumbers were observ ...200919490331
the central nervous system of sea cucumbers (echinodermata: holothuroidea) shows positive immunostaining for a chordate glial secretion.abstract:200919538733
a conserved ethylene biosynthesis enzyme leads to andromonoecy in two cucumis species.andromonoecy is a widespread sexual system in angiosperms, characterized by plants carrying both male and bisexual flowers. monoecy is characterized by the presence of both male and female flowers on the same plant. in cucumber, these sexual forms are controlled by the identity of the alleles at the m locus. in melon, we recently showed that the transition from monoecy to andromonoecy result from a mutation in 1-aminocyclopropane-1-carboxylic acid synthase (acs) gene, cmacs-7. to isolate the and ...200919578542
molecular characterization and pathogenicity of tomato yellow leaf curl virus in china.several tomato production regions in china were surveyed for tomato yellow leaf curl disease (tylcd), and 31 tomato leaf samples showing tylcd-like symptoms were collected. the partial or full-length genomes of these isolates were sequenced and tomato yellow leaf curl virus (tylcv) was detected in shanghai, zhejiang, jiangsu shandong and hebei provinces of china. the tylcv isolates found in china share high sequence identity ([98%) and have more than 97% sequence identity with tylcvil[ il:reo] ( ...200919590945
effects of cucumber mosaic virus infection on electron transport and antioxidant system in chloroplasts and mitochondria of cucumber and tomato leaves.we examined the responses of the photosynthetic and respiratory electron transport and antioxidant systems in cell organelles of cucumber (cucumis sativus l.) and tomato (lycopersicon esculentum mill.) leaves to infection of cucumber mosaic virus (cmv) by comparing the gas exchange, chl fluorescence, respiratory electron transport, superoxide dismutase (sod, ec 1.15.1.1) and ascorbate-glutathione (asa-gsh) cycle enzymes and the production of h(2)o(2) in chloroplasts, mitochondria and soluble fra ...200919140890
differential effects of triterpene glycosides, frondoside a and cucumarioside a2-2 isolated from sea cucumbers on caspase activation and apoptosis of human leukemia cells.frondoside a is a pentaoside having an acetyl moiety at the aglycon ring and xylose as a third monosaccharide residue. cucumarioside a(2)-2 is a pentaoside having glucose as a third monosaccahride unit. we compared the effects of frondoside a and a(2)-2 for cell death-inducing capability with close attention paid to structure-activity relationships. both frondoside a and a(2)-2 strongly induced apoptosis of leukemic cells. frondoside a-induced apoptosis was more potent and rapid than a(2)-2-indu ...200919162021
genetic background of host-pathogen interaction between cucumis sativus l. and pseudomonas syringae pv. lachrymans.the interplay of plant resistance mechanisms and bacterial pathogenicity is very complex. this applies also to the interaction that takes place between the pathogen pseudomonas syringae pv. lachrymans (smith et bryan) and the cucumber (cucumis sativus l.) as its host plant. research on p. syringae pv. lachrymans has led to the discovery of specific factors produced during pathogenesis, i.e. toxins or enzymes. similarly, studies on cucumber have identified the specific types of plant resistance e ...200919193976
an ecological risk assessment for triclosan in the terrestrial environment.triclosan (2,4,4'-trichloro-2'-hydroxydiphenyl ether) is a broad-spectrum bactericide used throughout north america and europe for a variety of antimicrobial functions. this paper addresses the risk to terrestrial organisms from several potential exposure pathways: exposure experienced by earthworms, terrestrial plants, and soil microorganisms as the result of the use of sewage sludge containing triclosan as an agricultural soil amendment; secondary exposure by birds and mammals from consumption ...200919228078
monitoring of pesticides and heavy metals in cucumber fruits produced from different farming systems.a total of 216kg of cucumber samples, representing three different types of farming production [e.g., conventional (c), greenhouse (g) and organic (o)], were collected from different locations in giza governorate (egypt), and subjected to pesticide residue and heavy metal analyses. residues of some organochlorine pesticides (ocps), such as hexachlorobenzene (hcb), heptachlor, aldrin, endrin, dieldrin and o,p'-ddt, as well as organophosphorus pesticides (opps), such as chlorpyrifos-methyl, thiome ...200919237184
[analysis of cdna library of cucumis sativus l. challenged by pseudomonas syringae pv. lachrymans].a cdna library was constructed from the leaves of the disease-resistant cucumber (cucumis sativus l.) cultivar 'd0462' challenged by pseudomonas syringae pv. lachrymans for 48 h. the inserted fragment sizes ranged from 0.45 to 2.1 kb and the average inserted size was 1 kb. sequencing analysis showed that 2 352 tuts (tentative unique transcripts), 282 contigs, and 2 070 singlets were identified in the 2 966 ests derived from the cdna library. the result of the blastx analysis indicated that there ...200919840927
a comparative study assaying commonly used sanitizers for antimicrobial activity against indicator bacteria and a salmonella typhimurium strain on fresh produce.with increased concerns over failures in vegetable and fruit sanitation, evaluating the efficacy of widely approved chemicals is ever more important. the purpose of this study was to determine whether sanitation treatments are equally effective against indicator bacteria and human enteric pathogens on cucumber and parsley. we provide here an experimental overview on the efficacy of common sanitation methods, which are based on peracetic acid-hydrogen peroxide, sodium dichloroisocyanurate, and th ...200919903410
differential response of antioxidant enzymes to cadmium stress in tolerant and sensitive cell line of cucumber (cucumis sativus l.).previously, a stable cell suspension of cucumber tolerant to 100 microm cdcl(2) was obtained (gzyl & gwóźdź, 2005, plant cell tissue organ cult 80: 59-67). in this study, the relationship between the activity of antioxidant enzymes and cadmium tolerance of cucumber cells was analyzed. a cadmium-sensitive and the cadmium-tolerant cell lines were exposed to 100 microm and 200 microm cdcl(2) and the activities of superoxide dismutase (sod), catalase (cat), ascorbate peroxidase (apox) and guaiacol p ...200920011684
study on the interaction between nucleic acids and imidacloprid and its application.the determination of imidacloprid with dna via a resonance light scattering (rls) technique was developed. the rls of dna was remarkably quenched after adding imidacloprid in aqueous medium of ph 2.10. an rls peak at 311 nm was found, and the quenched intensity of rls at this wavelength was proportional to the concentration of imidacloprid. the linear range of the calibration curve was approximately 0.02-2 microg/ml with the detection limit (s/n = 3) of 0.02 ng/ml. the imidacloprid in river wate ...200920183567
[contamination of soil with geohelminth eggs on vegetable organic farms in the lublin voivodeship, poland].organic farming, despite being more difficult and labour consuming than traditional farming, gains increasingly more followers among farmers. currently in poland there are approximately 10 000 organic farms. pure, uncontaminated soil in the lublin voivodeship makes this area an ideal location for organic agriculture production. in 2006-2007, 102 soil samples were examined from 40 organic farms specializing in vegetables and berries. farms for the study were selected by ecology- and food-producti ...200920209816
low oxygen consumption and high body content of catch connective tissue contribute to low metabolic rate of sea cucumbers.the energy consumption of echinoderms is low in comparison with that of other invertebrates. we demonstrated this by measuring the oxygen consumption rate per unit of body weight (vo2) of the sea cucumber actinopyga mauritiana: vo2 was 1/8 that of the "standard" invertebrates. low energy consumption in echinoderms has been attributed to their high skeletal content and to catch connective tissues (ccts) that maintain body posture by altering their mechanical properties with little energy expendit ...200919218491
effects of light quality on co2 assimilation, chlorophyll-fluorescence quenching, expression of calvin cycle genes and carbohydrate accumulation in cucumis sativus.light quality is thought to affect many plant physiological processes during growth and development, particularly photosynthesis. we examined how light quality influences plant photosynthesis by analyzing changes in photosynthetic parameters and expression levels of some photosynthesis related genes of cucumber (cucumis sativus l. cv. jinyou no. 1) plants. the plants were grown under different light qualities: purple (p), blue (b), green (g), yellow (y), red (r) and white light (w) of the same p ...200919410482
aroma evaluation of transgenic, thaumatin ii-producing cucumber fruits.fruits of transgenic cucumber lines expressing preprothaumatin ii gene were evaluated concerning their aroma. four homozygous lines, that is, 210 06, 212 01, 224 09, and 225 03 with different levels of transgene expression were selected. recipient line cv. borszczagowski, which was formed by inbred line of cucumis sativus l., was used as a control. the experiment was carried out in a greenhouse and an outdoor experimental plot. the aroma of cucumber fruits was evaluated by gc/ms, as well as gc/m ...200919397704
assessing the phytoavailability of dieldrin residues in charcoal-amended soil using tenax extraction.consecutive and single tenax extractions were applied to characterize the effectiveness of activated charcoal (ac) amendments to reduce the phytoavailability of dieldrin in a natively contaminated horticultural soil. dieldrin desorption from untreated and 800 mg(ac) kg(-1) soil was well described by a model with three dieldrin fractions of different kinetics: a rapidly (f(rap)), slowly (f(slow)), and very slowly (f(v.slow)) desorbing fraction. the ac amendment resulted in a transfer of dieldrin ...200919397375
chromatin structural rearrangement during dedifferentiation of protoplasts of cucumis sativus l.this paper reports on the structural rearrangement of satellite dna type i repeats and heterochromatin during the dedifferentiation and cell cycling of mesophyll protoplasts of cucumber (cucumis sativus). these repeats were localized in the telomeric heterochromatin of cucumber chromosomes and in the chromocenters of interphase nuclei. the dramatic reduction of heterochromatin involves decondensation of subtelomeric repeats in freshly isolated protoplasts; however, there are not a great many rem ...200919390825
a one-megabase physical map provides insights on gene organization in the enormous mitochondrial genome of cucumber.cucumber (cucumis sativus) has one of the largest mitochondrial genomes known among all eukaryotes, due in part to the accumulation of short 20 to 60 bp repetitive dna motifs. recombination among these repetitive dnas produces rearrangements affecting organization and expression of mitochondrial genes. to more efficiently identify rearrangements in the cucumber mitochondrial dna, we built two nonoverlapping 800 and 220 kb bac contigs and assigned major mitochondrial genes to these bacs. polymorp ...200919370086
chilling stress and mitochondrial genome rearrangement in the msc16 cucumber mutant affect the alternative oxidase and antioxidant defense system to a similar extent.the mosaic msc16 cucumber (cucumis sativus l.) mutant, which houses a rearranged mitochondrial genome, has altered respiratory chain activity, with a dysfunctional complex i, increased external nadh dehydrogenases (nd(ex)) activity, and a higher alternative oxidase (aox) capacity and aox protein level. in the present study, changes in oxidative defense metabolism resulting from the respiratory chain dysfunction in the msc16 mutant were compared with those induced by chilling. chilling increased ...200919549067
grafting of cucumis sativus onto cucurbita ficifolia leads to improved plant growth, increased light utilization and reduced accumulation of reactive oxygen species in chilled plants.the effects of chilling at 14 and 7 degrees c on plant growth, co(2) assimilation, light allocation, photosynthetic electron flux and antioxidant metabolism were examined in cucumber (cucumis sativus l. cv. jinyan no. 4, cs) plants with figleaf gourd (cucurbita ficifolia bouché, cf) and cucumber as rootstocks, respectively. growth inhibition by chilling at 7 degrees c was characterized by irreversible inhibition of co(2) assimilation in grafted plants with cucumber as rootstock and scion (cs/cs) ...200919521746
effects of exogenous putrescine on gas-exchange characteristics and chlorophyll fluorescence of nacl-stressed cucumber seedlings.the effects of 10 mm putrescine (put) treated by spraying on leaves on growth, chlorophyll content, photosynthetic gas-exchange characteristics, and chlorophyll fluorescence were investigated by growing cucumber plants (cucumis sativus l. cv. changchun mici) using hydroponics with or without 65 mm nacl as a salt stress. salt stress caused the reduction of growth such as leaf area, root volume, plant height, and fresh and dry weights. furthermore, net photosynthesis rate (p(n)), stomatal conducta ...200919507048
gene expression profiling of intestinal regeneration in the sea cucumber.among deuterostomes, the regenerative potential is maximally expressed in echinoderms, animals that can quickly replace most injured organs. in particular, sea cucumbers are excellent models for studying organ regeneration since they regenerate their digestive tract after evisceration. however, echinoderms have been sidelined in modern regeneration studies partially because of the lack of genome-wide profiling approaches afforded by modern genomic tools.for the last decade, our laboratory has be ...200919505337
changes of alternative oxidase activity, capacity and protein content in leaves of cucumis sativus wild-type and msc16 mutant grown under different light intensities.in vitro studies demonstrated that alternative oxidase (aox) is biochemically regulated by a sulfhydryl-disulfide system, interaction with alpha-ketoacids, ubiquinone pool redox state and protein content among others. however, there is still scarce information about the in vivo regulation of the aox. cucumis sativus wild-type (wt) and msc16 mutant plants were grown under two different light intensities and were used to analyze the relationship between the amount of leaf aox protein and its in vi ...200919493308
comparative analysis of marker-assisted and phenotypic selection for yield components in cucumber.theoretical studies suggest that marker-assisted selection (mas) has case-specific advantages over phenotypic selection (phe) for selection of quantitative traits. however, few studies have been conducted that empirically compare these selection methods in the context of a plant breeding program. for direct comparison of the effectiveness of mas and phe, four cucumber (cucumis sativus l.; 2n = 2x = 14) inbred lines were intermated and then maternal bulks were used to create four base populations ...200919484431
effects of phenylcarboxylic acids on mitosis, endoreduplication and expression of cell cycle-related genes in roots of cucumber (cucumis sativus l.).several benzoic and cinnamic acid derivatives were identified from cucumber root exudates. the effects of these phenylcarboxylic acids on root growth and cell cycle progression were examined in germinated seeds of cucumber. all 12 phenylcarboxylic acids (0.25 mm) tested significantly inhibited cucumber radicle growth, and cinnamic acid exerted a dose-dependent inhibitory effect. at 6 h after exposure to the acids, transcript levels of the cell cycle-related genes, including two cyclin-dependent ...200919459009
[influences of heavy metal cadmium alone and in combination with zinc on the growth and activities of antioxidant enzymes of cucumis sativus hairy roots].the effects of heavy metal cadmium (cd), alone and in combination with zinc (zn), on the root growth and activity of antioxidant enzymes superoxide dismutase (sod) and peroxidase (pod) in cucumis sativus l. hairy roots were studied. the purpose was to study the possibilities on using c. sativus hairy roots for phytoremediation of cadmium contamination. the results showed that less than 10 mg/l cd enhanced the growth of c. sativus hairy roots and increased root diameter only in 5-15 days of root ...200919441228
beneficial effects of exogenous selenium in cucumber seedlings subjected to salt stress.the study was conducted in order to determine the effects of exogenous selenium (se) supply (5, 10, or 20 μm) on the resistance of cucumber (cucumis sativus l.) cv. polan f1 seedlings to salt stress (50 mm nacl). plant growth was negatively affected by excessive salinity and dry mass production as well as photosynthetic pigments accumulation severely decreased. se treatments at 5 and 10 μm significantly improved the growth rate and increased the photosynthetic pigments and proline contents in cu ...200919434374
selective trans-cinnamic acid uptake impairs [ca2+]cyt homeostasis and growth in cucumis sativus l.to obtain insight into interspecies interactions mediated by allelochemicals, the response of cucumber (cucumis sativus l. cv jinyan no.4) and figleaf gourd (cucurbita ficifolia bouché) seedlings to trans-cinnamic acid (ca) (1) was investigated. while trans-ca is an autotoxin in cucumber root exudates, figleaf gourd is resistant to it. cucumber, however, had a high rate of trans-ca uptake by the roots, leading to reduced root growth. the trans-ca treatment also resulted in an intracellular relea ...200920063205
primary trisomics obtained from autotriploid by diploid reciprocal crosses in cucumber.to promote cytogenetical studies on cucumber (cucumis sativus l., 2n = 2x = 14), the reciprocal crosses were made between autotriploid and diploid for selecting the primary trisomics. meanwhile, chromosome behavior during meiosis in autotriploid cucumber was investigated to look for cytological evidences for origin of primary trisomics. many viable f(1) seeds were obtained from reciprocal crosses between autotriploid and diploid. the number of chromosomes of 56 surviving progenies varied from 14 ...200920033455
synthesis and phytotoxic activity of new pyridones derived from 4-hydroxy-6-methylpyridin-2(1h)-one.commercial dehydroacetic acid was converted into 4-hydroxy-6-methylpyridin-2(1h)-one (3), which was then condensed with several aliphatic aldehydes to produce seven new title compounds in variable yields (35-92%). reaction of 3 with alpha,beta-unsaturated aldehydes resulted in the formation of condensed pyran derivatives 4g' and 4h'. a mechanism is proposed to explain the formation of such compounds. the effects of all methylpyridin-2(1h)-one derivatives on the development of the dicotyledonous ...200920032871
cool as a cucumber.the genome of the seventh plant to be sequenced, cucumis sativus l., was assembled using the conventional long-read sanger sequencing and higher-throughput short-read technology. this genome is the entry point for exploring the diversity and function of the cucurbitaceae family of agriculturally important plants. its compact genome, without evidence of recent duplication, will be useful in comparative analysis of plant genome evolution.200919935759
relationship between dieldrin uptake in cucumber and solvent-extractable residue in soil.to prevent the distribution of cucumbers with dieldrin contamination exceeding the limit set by the japanese food sanitation law, the extraction solvent for dieldrin-contaminated soil was selected prior to cultivation so that the dieldrin residue level in cucumber could be predicted. the exhaustive extraction from soil could not explain the dieldrin uptake by cucumber plants. however, significant correlation (r(2) = 0.966, p < 0.001) was observed between dieldrin concentrations in cucumber and d ...200919904933
[effects of different water and fertilizer supply on cucumber soil nutrient content, enzyme activity, and microbial diversity].with cucumber (cucumis sativus l.) variety jinyou 1 as test material, a greenhouse experiment was conducted to study the effects of different water and fertilizer supply on the cucumber soil nutrient content, enzyme activity, and microbial diversity. three water regimes (50%-60%, 70%-80%, and 90%-100% soil relative moisture content) and two fertilization practices (600 kg n x hm(-2) + 420 kg p2o5 x hm(-2) and 420 kg n x hm(-2) + 294 kg p2o5 x hm(-2)) were designed. the increase of water and fert ...200919899470
synthesis and biological evaluation of new ozonides with improved plant growth regulatory activity.the iron oxyallyl carbocation generated from 2,7-dibromocycloheptanone was induced to undergo [4 + 3] cycloaddition reactions with various furans, affording a series of 12-oxatricyclo-[4.4.1.1(2,5)]-dodec-3-en-11-one adducts. similar methodology was used to prepare two additional cycloadducts using menthofuran and two homologous aliphatic dibromoketones. dipolar cycloaddition of ozone to the adducts afforded the corresponding secondary ozonides (i.e., 1,2,4-trioxolanes) in variable yields. ozoni ...200919886678
assessment of the dermal exposure to azoxystrobin among women tending cucumbers in selected polish greenhouses after restricted entry intervals expired--the role of the protective gloves.the purpose of our study was to determine the level of skin contamination by azoxystrobin in a group of women tending cucumbers in a vegetable-growing greenhouse after restricted entry intervals expired.200919819834
comparison of hs-sdme with spme and spe for the determination of eight organochlorine and organophosphorus pesticide residues in food matrices.a headspace single-drop microextraction (hs-sdme) procedure is optimized for the analysis of organochlorine and organophosphorous pesticide residues in food matrices, namely cucumbers and strawberries by gas chromatography with an electron capture detector. the parameters affecting the hs-sdme performance, such as selection of the extraction solvent, solvent drop volume, extraction time, temperature, stirring rate, and ionic strength, were studied and optimized. extraction was achieved by exposi ...200919772747
brassinosteroids promote photosynthesis and growth by enhancing activation of rubisco and expression of photosynthetic genes in cucumis sativus.brassinosteroids (brs) are a new group of plant growth substances that promote plant growth and productivity. we showed in this study that improved growth of cucumber (cucumis sativus) plants after treatment with 24-epibrassinolide (ebr), an active br, was associated with increased co(2) assimilation and quantum yield of psii (phi(psii)). treatment of brassinazole (brz), a specific inhibitor for br biosynthesis, reduced plant growth and at the same time decreased co(2) assimilation and phi(psii) ...200919760261
laser ablation icp-ms reveals patterns of copper differing from zinc in growth zones of cucumber roots.laser ablation coupled with inductively coupled plasma-mass spectrometry was used to find cu and zn concentration in surface tissue along a longitudinal developmental gradient with meristem, rapidly elongating tissue, and nongrowing tissue in a model system of seedling roots of cucumis sativus l. (cucumber). tissue metal accumulation was determined for roots of seedlings growing on cellulosic germination paper treated with nutrient solution (controls), and also treated with concentrations of zn ...200919148675
melatonin applied to cucumber (cucumis sativus l.) seeds improves germination during chilling stress.the relationship between germination and melatonin applied during osmo- and hydropriming was studied in cucumber seeds. the proportion of nuclei with different dna contents, the mean ploidy and the (2c + 4c = 8c)/2c ratio in unprimed and primed, dry and imbibed at 10 degrees c seeds were established by flow cytometry. thiobarbituric acid reactive substances and protein oxidation were also estimated. melatonin and indole-3-acetic acid (iaa) concentrations in the seeds were determined using high-p ...200919141087
synthesis and phytogrowth properties of oxabicyclic analogues related to helminthosporin.this investigation describes the synthesis and biological evaluation of a series of oxabicyclic analogues related to the helminthosporins. four oxabicycles were prepared by [4+3] cycloaddition of an oxyallyl carbocation, generated in situ from 2,4-dibromopentan-3-one, with selected furans. functional group manipulations of the oxabicyclic architecture generated nine further derivatives. the phytotoxic properties of these oxabicycles were evaluated as their ability to interfere with the growth of ...200919127245
regeneration of the radial nerve cord in the sea cucumber holothuria glaberrima.regeneration of neurons and fibers in the mammalian spinal cord has not been plausible, even though extensive studies have been made to understand the restrictive factors involved. new experimental models and strategies are necessary to determine how new nerve cells are generated and how fibers regrow and connect with their targets in adult animals. non-vertebrate deuterostomes might provide some answers to these questions. echinoderms, with their amazing regenerative capacities could serve as m ...200919126208
synthesis and herbicidal activity of 12-(aryloxyacyloxyimino)-1,15-pentadecanlactone derivatives.a series of novel 12-(aryloxyacyloxyimino)-1,15-pentadecanlactone derivatives (3) were synthesized, and their structures including configuration of c=n bond were confirmed by (1)h nmr, elemental analysis and x-ray diffraction analysis. the bioassay showed that some of them exhibited excellent herbicidal activity against amaranthus tricolor l. the activity of compounds 3 except compounds 3a1-2 was much higher than the commercial herbicide 2,4-d and the activity of about half of compounds 3 was co ...200919117417
seasonal variation in fruit and vegetable consumption in a rural agricultural community.seasonal variation in fruit and vegetable consumption has been documented in a limited number of previous investigations and is important for the design of epidemiologic investigations and in the evaluation of intervention programs.200919103322
triterpene glycosides from antarctic sea cucumbers. 2. structure of achlioniceosides a(1), a(2), and a(3) from the sea cucumber achlionice violaecuspidata (=rhipidothuria racowitzai).three new triterpene glycosides, achlioniceosides a(1) (1), a(2) (2), and a(3) (3), have been isolated from the antarctic sea cucumber achlionice violaecuspidata. the glycoside structures were elucidated using extensive nmr spectroscopic analysis including one-dimensional (1)h and (13)c spectra, (1)h-(1)h-cosy, hmbc, hmqc, and noesy and mass spectrometry. gycosides 1-3 are disulfated pentaosides that are branched at the first xylose residue. the sulfates are attached to c-6 of the glucose residu ...200919072534
elucidation of molecular diversity and body distribution of saponins in the sea cucumber holothuria forskali (echinodermata) by mass spectrometry.sea cucumbers contain triterpene glycosides called saponins. we investigated the complex saponin mixture extracted from the common mediterranean species holothuria forskali. two different body components were analyzed separately: the body wall (which protects the animal and is moreover the most important organ in terms of surface and weight) and the cuvierian tubules (a defensive organ that can be expelled on predators in response to an attack). maldi/ms and maldi/ms/ms were used to detect sapon ...200919036349
exposure to dust and endotoxin of employees in cucumber and tomato nurseries.exposure to bioaerosols in occupational settings is associated with a range of adverse health effects. the aim of this study was to investigate the exposure levels to dust and endotoxin of people working in two cucumber nurseries and two tomato nurseries. exposure was measured for greenhouse workers (n = 70) mainly working on harvesting cucumbers and tomatoes and clearing the plants after the harvest season. the people were exposed to between 0.2 and 15 mg inhalable dust m(-3) (median = 1.6 mg m ...200919033558
expression pattern of the pre-prothaumatin ii gene under the control of the camv 35s promoter in transgenic cucumber (cucumis sativus l.) flower buds and fruits.thaumatin ii is an extremely sweet-tasting protein produced by fruits of the west african shrub thaumatococcus daniellii benth, so it can be used in biotechnology to improve the tastes of various plant products. this study is concerned with the spatial and temporal aspects of expression of the 35s-pre-prothaumatin ii chimeric gene in flower buds and fruits of transgenic cucumber (cucumis sativus l.) line 225. the activity of the 35s promoter in organs of line 225 was compared with its activity i ...200919193977
iron availability affects the function of mitochondria in cucumber roots.* in strategy-i-plants, iron (fe) deficiency induces processes leading to increased fe solubilization in the rhizosphere, including reduction by ferric reductases and active proton extrusion. these processes require active respiration to function. in this work we investigated the effect of fe deficiency on respiratory activities of cucumber (cucumis sativus) roots. * we compared oxygen consumption rate and the activities of the respiratory chain complexes on purified mitochondria from roots grow ...200919192186
bn-page analysis of the respiratory chain complexes in mitochondria of cucumber msc16 mutant.rearrangements of mitochondrial dna in msc16 mutant of cucumber (cucumis sativus l.) affect mitochondrial functioning due to the alteration mainly of complex i resulting in several metabolic changes. one-dimensional blue-native polyacrylamide gel electrophoresis (bn-page) and densitometric measurements showed that the level and in-gel capacity of complex i were lower in msc16 leaf and root mitochondria as compared to wild-type (wt). the level and capacity of supercomplex i+iii(2) were always low ...200919181534
the research progress of antitumorous effectiveness of stichopus japonicus acid mucopolysaccharide in north of china.the sea cucumbers growing in the estuary of the pohai of northern china are called stichopus japonicus and are the orthodox holothurians in traditional chinese medicine. there are multiple biological active ingredients in s. japonicus, and s. japonicus acid mucopolysaccharide (sjamp) is one of the important ingredients. sjamp has multiple pharmacologic actions, such as antitumor, immunologic regulation, anticoagulated blood, and antivirus. the research on antitumor has been carried out by way of ...200919174693
preparation of achiral and chiral (e)-enaminopyran-2,4-diones and their phytotoxic activity.a short and efficient approach to a range of new chiral and achiral functionalized (e)-enaminopyran-2,4-diones starting with commercially available dehydroacetic acid is described. the phytotoxic properties of these (e)-enaminopyran-2,4-diones were evaluated by their ability to interfere with the growth of sorghum bicolor and cucumis sativus seedlings. a different sensitivity of the two crops was evident with the (e)-enaminopyran-2,4-diones. the most active compounds were also tested against two ...200919170511
effect of additives in aqueous formulation on the foliar uptake of dimethomorph by cucumbers.the efficacy enhancement of dimethomorph formulation by several adjuvants is thought to be through increased foliar uptake. in order to identify the most effective adjuvants, the adjuvancy of 36 additives was examined in aqueous formulations in relation to the absorption of dimethomorph by cucumber leaves.200919165749
the genome of the cucumber, cucumis sativus l.cucumber is an economically important crop as well as a model system for sex determination studies and plant vascular biology. here we report the draft genome sequence of cucumis sativus var. sativus l., assembled using a novel combination of traditional sanger and next-generation illumina ga sequencing technologies to obtain 72.2-fold genome coverage. the absence of recent whole-genome duplication, along with the presence of few tandem duplications, explains the small number of genes in the cuc ...200919881527
centromere repositioning in cucurbit species: implication of the genomic impact from centromere activation and inactivation.the centromere of an eukaryotic chromosome can move to a new position during evolution, which may result in a major alteration of the chromosome morphology and karyotype. this centromere repositioning phenomenon has been extensively documented in mammalian species and was implicated to play an important role in mammalian genome evolution. here we report a centromere repositioning event in plant species. comparative fluorescence in situ hybridization mapping using common sets of fosmid clones bet ...200919706458
identification of host attractants for the ethiopian fruit fly, dacus ciliatus loew.the ethiopian fruit fly, dacus ciliatus, is an oligophagous pest of cucurbit crops, particularly melons, cucumbers, and marrows (summer squash). the present study aimed to identify host attractants for d. ciliatus and was guided by a behavioral bioassay and an electrophysiological assay. we tested volatile compounds from the fruits of a host plant, ripe and unripe galia melon, cucumis melo var. reticulates. both sexes were attracted to melon volatiles. those of ripe melon were preferred. gas chr ...200919440796
species and genus specificity of the intergenic spacer (igs) in the ribosomal rna genes of cucurbitaceae.the use of intergenic spacer (igs) fragments of plant ribosomal dna (rdna) for the differentiation between genera and species is tested by cross-hybridization experiments with different igs probes of two cucurbitaceae, cucurbita pepo (zucchini) and cucumis sativus (cucumber). hybridization with cloned fragments of different parts of the igs of ribosomal dna exhibit a different degree of conservation within and between the cucurbitaceae genera. in general, cucurbita species seem to be closer rela ...20092631730
the cucurbitaceae and solanaceae illustrated in medieval manuscripts known as the tacuinum sanitatis.beginning in the last two decades of the 14th century, richly illuminated versions of the tacuinum sanitatis, the latin translation of an 11th-century arabic manuscript known as taqwim al-sihha bi al-ashab al-sitta, were produced in northern italy. these illustrated manuscripts provide a window on late medieval life in that region by containing some 200 full-page illustrations, many of which vividly depict the harvest of vegetables, fruits, flowers, grains, aromatics and medicinal plants. our ob ...200919318382
sequencing of 6.7 mb of the melon genome using a bac pooling strategy.cucumis melo (melon) belongs to the cucurbitaceae family, whose economic importance among horticulture crops is second only to solanaceae. melon has a high intra-specific genetic variation, morphologic diversity and a small genome size (454 mb), which make it suitable for a great variety of molecular and genetic studies. a number of genetic and genomic resources have already been developed, such as several genetic maps, bac genomic libraries, a bac-based physical map and est collections. sequenc ...201021073723
[expression analysis of the unisex-determine gene m in cucumber].sex determination in plants has various mechanism; however, a single gene locus controlling unisexual expression is unique in the cucurbitaceae plants, particularly cucumber (cucumis sativus l.) and melon (cucumis melo l.). in this study, with quantitative rt-pcr method, two sets of near-isogenic lines (nils) were used to analyze the expression pattern of gene csacs2 (genbank accession number fj529216). additionally, chemical applications (agno3 and avg) were used to investigate the effect of th ...201020566468
antioxidant potential and phenolic acid content of certain cucurbitaceous plants cultivated in egypt.many species belonging to cucurbitaceae family have long been regarded as food, medicinal plants, or both. preliminary phytochemical screening of citrullus colocynthis l., cucumis sativus l. and momordica charantia l. herbs showed the presence of phenolic compounds. quantitative analysis of total phenolic compounds using folin-ciocalteu reagent revealed the presence of 50.87 mg gae g(-1), 56.58 mg gae g(-1) and 42.36 mg gae g(-1) in c. colocynthis l., c. sativus l. and m. charantia l. herbs, res ...201020835955
silicon enhances natural enemy attraction and biological control through induced plant defences.silicon (si) is known to have a role in constitutive plant defence against arthropod pests, and recent work has illustrated involvement in induced plant defences. the present tri-trophic study tested the hypothesis that si increases natural enemy attraction to pest-infested plants and improves biological control. cucumber plants treated with potassium silicate (si+) and untreated control plants (si-) were maintained in separately vented glasshouse compartments. y-tube olfactometer studies showed ...201019737442
x-ray absorption spectroscopy (xas) corroboration of the uptake and storage of ceo(2) nanoparticles and assessment of their differential toxicity in four edible plant species.fate, transport, and possible toxicity of cerium oxide nanoparticles (nanoceria, ceo(2)) are still unknown. in this study, seeds of alfalfa (medicago sativa), corn (zea mays), cucumber (cucumis sativus), and tomato (lycopersicon esculentum) were treated with nanoceria at 0-4000 mg l(-1). the cerium uptake and oxidation state within tissues were determined using inductively coupled plasma-optical emission spectroscopy (icp-oes) and x-ray absorption spectroscopy (xas), respectively. the germinatio ...201020187606
qualitative and quantitative saponin contents in five sea cucumbers from the indian ocean.to avoid predation, holothuroids produce feeding-deterrent molecules in their body wall and viscera, the so-called saponins. five tropical sea cucumber species of the family holothuriidae were investigated in order to study their saponin content in two different organs, the body wall and the cuvierian tubules. mass spectrometry techniques (maldi- and esi-ms) were used to detect and analyze saponins. the smallest number of saponins was observed in holothuria atra, which contained a total of four ...201020161976
selenium modifies the effect of short-term chilling stress on cucumber plants.the objective of this study was to investigate the effect of selenium (se) supply (0, control; 2.5, 5, 10, or 20 μm) on cucumber (cucumis sativus l.) cv. polan f1 plants grown under short-term low temperature stress. about 14-16 day-old seedlings, grown at an optimal temperature (25/20°c; day/night), were exposed to short-term chilling stress with a day/night temperature of 10°c/5°c for 24 h, for a further 24 h at 20°c/15°c, and then transferred to 25/20°c (re-warming) for 7 days. se did not aff ...201020140652
continuous uv-b irradiation induces endoreduplication and peroxidase activity in epidermal cells surrounding trichomes on cucumber cotyledons.most trichomes on the surface of cucumber (cucumis sativus l.) cotyledons consist of three cells. we previously showed that continuous uv-b (290-320 nm) irradiation induces rapid cellular expansion and the accumulation of polyphenolic compounds, possibly stress lignin, in epidermal cells around these trichomes.(1)) to examine the mechanism of the uv-b-induced cellular expansion and to determine which step is stimulated by uv-b irradiation in the lignin synthesis pathway, we investigated relative ...201020110622
purification and characterization of pepsin-solubilized collagen from skin and connective tissue of giant red sea cucumber (parastichopus californicus).pepsin-solubilized collagen (psc) was extracted from giant red sea cucumbers ( parastichopus californicus ) and characterized for denaturation temperature (t(d)), maximum transition temperature (t(m)), enzyme-digested peptide maps, and gel-forming capability. sds-page showed that pscs from giant red sea cucumber skin and connective tissue were both type i collagens, consisting of three alpha(1) chains of approximately 138 kda each. the amino acid composition and peptide maps of pscs digested by ...201020085374
risk assessment of pesticides and heavy metals contaminants in vegetables: a novel bioassay method using daphnia magna straus.cucumber and potato samples of known levels of pesticides and heavy metal residues, as respectively measured by gas chromatography and atomic absorption, were subjected to a bioassay method using daphnia magna in order to assess the potential of the toxic hazard of their contaminants. based on the estimated lethal time for 50% mortality (lt50) in daphnids, we suggested a classification to categorize toxic hazards in six definite ratings. either samples of cucumbers (from conventional, greenhouse ...201019853633
identification and mapping of molecular markers linked to the tuberculate fruit gene in the cucumber (cucumis sativus l.).warty fruit is one of the highly valuable external quality traits related to the market values of cucumber. genetic analysis has shown that a single dominant gene, tu (tuberculate fruit), determines the warty fruit trait in the cucumber plant. an f(2) population (247 individuals) from the cross of s06 x s52 was used for the mapping of the tu/tu locus. by combining bulked segregant analysis with the sequence-related amplified polymorphism (srap) and simple sequence repeat (ssr) markers, 15 marker ...201019847386
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