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sampling stink bugs (hemiptera: pentatomidae) for population estimation and pest management in southeastern cotton production.stink bugs (hemiptera: pentatomidae) were sampled in commercial cotton, gossypium hirsutum l., fields in 2007 and 2008 in south carolina and georgia. the main species collected with the beat cloth and sweep net methods were green stink bug, acrosternum hilare (say) (63 and 57%, respectively); brown stink bug, euschistus servus (say) (23 and 18%, respectively); and southern green stink bug, nezara viridula (l.) (14 and 22%, respectively). average stink bug densities were 0.145 +/- 0.010 (mean +/- ...200920069868
an evaluation of eco-friendly naturally coloured cottons regarding seed cotton yield, yield components and major lint quality traits under conditions of east mediterranean region of turkey.in the study carried out in 2002-2003 in the east mediterranean region of turkey (in kahramanmaras province), four different naturally coloured cotton (gossypium hirsutum l.) (dark brown, light brown, cream and green) lines from azerbaijan and two white linted cotton varieties (maras-92 and sayar-314 (g. hirsutum l.)) of the region were used as material. the aim of this study was to determine seed cotton yield and yield components and major lint quality traits of investigated coloured cotton lin ...200920128501
a cotton (gossypium hirsutum) dre-binding transcription factor gene, ghdreb, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat.a cotton (g. hirsutum l.) dehydration responsive element binding protein gene, ghdreb, which encodes a 153 amino acid protein containing a conserved ap2/erebp domain, was isolated from the cdna library of cotton cv. simian 3 by a yeast one-hybrid system. rna blot analysis showed that the ghdreb gene was induced in cotton seedlings by drought, high salt and cold stresses. an electrophoretic mobility shift assay (emsa) indicated that the ghdreb protein bound specifically to the dre core element (a ...200919005655
cotton metallothionein ghmt3a, a reactive oxygen species scavenger, increased tolerance against abiotic stress in transgenic tobacco and yeast.a cdna clone encoding a 64-amino acid type 3 metallothionein protein, designated ghmt3a, was isolated from cotton (gossypium hirsutum) by cdna library screening. northern blot analysis indicated that mrna accumulation of ghmt3a was up-regulated not only by high salinity, drought, and low temperature stresses, but also by heavy metal ions, abscisic acid (aba), ethylene, and reactive oxygen species (ros) in cotton seedlings. transgenic tobacco (nicotiana tabacum) plants overexpressing ghmt3a showe ...200919033550
scanning electron microscopy applied to seed-borne fungi examination.the aim of this study was to test the standard scanning electron microscopy (sem) as a potential alternative to study seed-borne fungi in seeds, by two different conditions of blotter test and water restriction treatment. in the blotter test, seeds were subjected to conditions that enabled pathogen growth and expression, whereas the water restriction method consisted in preventing seed germination during the incubation period, resulting in the artificial inoculation of fungi. in the first condit ...200919204924
identification and expression of a new delta-12 fatty acid desaturase (fad2-4) gene in upland cotton and its functional expression in yeast and arabidopsis thaliana plants.a cotton (gossypium hirsutum l.) genomic clone encompassing a 17.9-kb dna fragment was found to contain a delta-12 fatty acid desaturase gene (designated fad2-4). the fad2-4 open reading frame has 1,155bp and is uninterrupted, encoding a conceptual fad2-4 polypeptide of 384 amino acids that has 98% identity with the cotton fad2-3 polypeptide. the fad2-4 gene has a single intron of 2,780 bp in its 5'-untranslated region (5'-utr). the 3'-flanking regions and 5'-utr introns in the fad2-4 and fad2-3 ...200919217793
temporal analysis of cotton boll symptoms resulting from southern green stink bug feeding and transmission of a bacterial pathogen.the southern green stink bug, nezara viridula (l.), is a significant pest of cotton, gossypium hirsutum l., and is becoming an increasing challenge due to the decrease in use of broad-spectrum insecticides on the crop. the southern green stink bug can vector an opportunistic pantoea agglomerans strain (designated sc 1-r) into cotton bolls, resulting in infection. the appearance of stink bug damage varies, and pest managers cannot readily identify its source. this research reports a systematic de ...200919253615
comparisons of boll weevil (coleoptera: curculionidae) pheromone traps with and without kill strips.boll weevil, anthonomus grandis grandis boheman (coleoptera: curculionidae), eradication programs typically equip pheromone traps with an insecticide-impregnated kill strip. these strips are intended to kill captured insects, thereby simplifying trap servicing and reducing the loss of weevils from predation and escape. however, the effectiveness of kill strips has not been extensively evaluated. we examined the influences of kill strips on weevil captures, trap servicing, and the incidences of w ...200919253635
can the amount of corn acreage predict fall armyworm (lepidoptera: noctuidae) infestation levels in nearby cotton?fall armyworm, spodoptera frugiperda (j.e. smith) (lepidoptera: noctuidae), is a major pest of corn, zea mays l., and a significant, but more sporadic, pest of cotton, gossypium hirsutum l., in the western hemisphere. previous studies showed that the cotton infestations primarily involve a fall armyworm subpopulation known as the "corn-strain" for which corn is the preferred host plant. it was suggested that the fall armyworm infesting cotton originated in corn and spread into secondary hosts as ...200919253639
bacillus thuringiensis cry1ac resistance frequency in tobacco budworm (lepidoptera: noctuidae).the tobacco budworm, heliothis virescens (f.) (lepidoptera: noctuidae), is one of the most important pests of cotton, gossypium hirsutum l, that has become resistant to a wide range of synthetic insecticides. crylac-expressing cotton has proven its effectiveness against this insect since its introduction in north america in 1996. however, the constant exposure of tobacco budworm to this protein toxin may result in the development of resistance to it. to estimate the frequency of alleles that con ...200919253658
expressions of three cotton genes encoding the pip proteins are regulated in root development and in response to stresses.cotton (gossypium hirsutum), the most important textile crop worldwide, often encounters water stress such as drought or waterlog during its growth season (summer). to investigate molecular mechanism of water regulation in cotton plants, three cdnas encoding the plasma membrane intrinsic protein (pip) were isolated from cotton root cdna library, and designated ghpip1;1, ghpip2;1 and ghpip2;2, respectively. all of the three pip proteins displayed water channel activity in xenopus laevis oocytes. ...200918956193
production of mrna from the cry1ac transgene differs among bollgard lines which correlates to the level of subsequent protein.commercial cultivars of bollgard cotton, gossypium hirsutum l., differ in the amount of expressed cry1ac protein. however, the plant-mechanism for which this occurs is still unknown. using quantitative real-time polymerase chain reaction (qpcr), we developed a method to determine if differences in the overall level of cry1ac among bollgard lines could be correlated to the mrna transcripts. our data shows that the cry1ac mrna transcript differs among bollgard lines and are correlated with corresp ...200918594999
molecular cloning and characterization of an inducible rna-dependent rna polymerase gene, ghrdrp, from cotton (gossypium hirsutum l.).the rna-dependent rna polymerase (rdrp) cdna, designated as gossypium hirsutum rdrp (ghrdrp) was cloned from cotton by rapid amplification of cdna ends-polymerase chain reaction (race-pcr). the full-length cdna was 3,672 bp in size and encoded an open reading frame (orf) of 1,110 amino acids which contained the rdrp conserved functional domain and the signature motif dbdgd. amino acid sequence alignment indicated that ghrdrp shared the highest identity (66.37%) with atrdrp1 and had homology with ...200917929195
reproduction of meloidogyne marylandi and m. incognita on several poaceae.the susceptibility of 22 plant species to meloidogyne marylandi and m. incognita was examined in three greenhouse experiments. inoculum of m. marylandi was eggs from cultures maintained on zoysia matrella "cavalier" or cynodon dactylon x c. trasvaalensis "tifdwarf". inoculum of m. incognita was eggs from cultures maintained on solanum lycopersicum 'rutgers'. in each host test the inoculum density was 2,000 nematode eggs/pot. none of the three dicot species tested (gossypium hirsutum, arachis hyp ...200922661770
sucrose synthase affects carbon partitioning to increase cellulose production and altered cell wall ultrastructure.overexpression of the gossypium hirsutum sucrose synthase (susy) gene under the control of 2 promoters was examined in hybrid poplar (populus alba x grandidentata). analysis of rna transcript abundance, enzyme activity, cell wall composition, and soluble carbohydrates revealed significant changes in the transgenic lines. all lines showed significantly increased susy enzyme activity in developing xylem. this activity manifested in altered secondary cell wall cellulose content per dry weight in al ...200919625620
the multi-year effects of repeatedly growing cotton with moderate resistance to meloidogyne incognita.meloidogyne incognita causes more damage to cotton in the us than any other pathogen. the objective of this study was to document the cumulative effect of moderate resistance on m. incognita population density, root galling, and yield suppression in the southern united states on a moderately resistant cotton genotype grown continuously for three years. cotton genotypes were phytogen ph98-3196 (77% suppression of m. incognita), acala nemx (85% suppression of m. incognita), and delta and pine land ...200922661787
analysis of cotton (gossypium hirsutum) root proteomes during a compatible interaction with the black root rot fungus thielaviopsis basicola.a proteomic approach was used to uncover the inducible molecular defense mechanism of cotton root occurring during the compatible interaction with thielaviopsis basicola. microscopic observation of cotton root inoculated with a suspension of conidia showed that this necrotrophic hemibiotroph fungus interacts with the plant and completes its life cycle in our experimental system. 2-de analysis of root extracts taken after 1, 3, 5, and 7 days postinoculation and cluster analysis of the protein exp ...200919105169
population dynamics of meloidogyne arenaria and pasteuria penetrans in a long-term crop rotation study.the endospore-forming bacterium pasteuria penetrans is an obligate parasite of root-knot nematodes (meloidogyne spp.). the primary objective of this study was to determine the effect of crop sequence on abundance of p. penetrans. the experiment was conducted from 2000 to 2008 at a field site naturally infested with both the bacterium and its host meloidogyne arenaria and included the following crop sequences: continuous peanut (arachis hypogaea) (p-p-p) and peanut rotated with either 2 years of ...200922736828
sampling nucleotide diversity in cotton.cultivated cotton is an annual fiber crop derived mainly from two perennial species, gossypium hirsutum l. or upland cotton, and g. barbadense l., extra long-staple fiber pima or egyptian cotton. these two cultivated species are among five allotetraploid species presumably derived monophyletically between g. arboreum and g. raimondii. genomic-based approaches have been hindered by the limited variation within species. yet, population-based methods are being used for genome-wide introgression of ...200919840401
total and percent atropisomers of gossypol and gossypol-6-methyl ether in seeds from pima cottons and accessions of gossypium barbadense l.gossypol occurs naturally in the seed, foliage, and roots of the cotton plant ( gossypium ) as atropisomers due to restricted rotation around the binaphthyl bond. the atropisomers differ in their biological activities. (-)-(r)-gossypol is more toxic and exhibits significantly greater anticancer activity than the (+)-(s)-atropisomer. most commercial upland ( gossypium hirsutum ) cottonseeds have an (r)- to (s)-gossypol ratio of approximately 2:3, but some pima ( gossypium barbadense ) seeds have ...200919113939
pb(ii) biosorption from hazardous aqueous streams using gossypium hirsutum (cotton) waste biomass.studies on the biosorptive ability of gossypium hirsutum (cotton) waste biomass outlined that smaller size of biosorbent (0.355mm), higher biomass dose (0.20g), 5 ph and 100mg/l initial pb(ii) concentration were more suitable for enhanced pb(ii) biosorption from aqueous medium. the langmuir isotherm model and pseudo second order kinetic model fitted well to the data of pb(ii) biosorption. highly negative magnitude of gibbs free energy (deltag degrees ) indicated that the process was spontaneous ...200918502037
purification and biochemical characterization of polyphenol oxidases from embryogenic and nonembryogenic cotton (gossypium hirsutum l.) cells.polyphenol oxidases (ppos) were isolated from cell suspensions of two cultivars of cotton (gossypium hirsutum l.), and their biochemical characteristics were studied. ppo from coker 312, an embryogenic cultivar, showed a highest affinity to catechol 20 mm, and ppo from r405-2000, a nonembryogenic cultivar, showed a highest affinity to 4-methylcatechol 20 mm. the optimal ph for ppo activity was 7.0 and 6.0 for coker 312 and r405-2000, respectively. the enzyme had an optimal temperature of 25 degr ...200918795241
analysis of xyloglucan endotransglycosylase/hydrolase (xth) genes from allotetraploid (gossypium hirsutum) cotton and its diploid progenitors expressed during fiber elongation.multiple cellular pathways have been shown to be involved during fiber initiation and elongation stages in the cultivated allotetraploid cotton (gossypium hirsutum). the cell wall enzymes xyloglucan endotransglycosylase/hydrolases (xth) have been reported to be associated with the biosynthesis of the cell wall and the growth of cotton fibers, probably regulating the plasticity of the primary cell wall. among various cotton fiber cdnas found to be preferentially expressed in cotton fibers, a xylo ...200918789555
down-regulation of ghadf1 gene expression affects cotton fibre properties.cotton fibre is the most important natural fibres for textile industry. to date, the mechanism that governs the development of fibre traits is largely unknown. in this study, we have characterized the function of a member of the actin depolymerizing factor (adf) family in gossypium hirsutum by down-regulation of the gene (designated as ghadf1) expression in the transgenic cotton plants. we observed that both the fibre length and strength of the ghadf1-underexpressing plants increased as compared ...200918761653
construction of a bacterial artificial chromosome library of tm-1, a standard line for genetics and genomics in upland cotton.a bacterial artificial chromosome (bac) library was constructed for gossypium hirsutum acc. tm-1, a genetic and genomic standard line for upland cotton. the library consists of 147 456 clones with an average insert size of 122.8 kb ranging from 97 to 240 kb. about 96.0% of the clones have inserts over 100 kb. therefore, this library represents theoretically 7.4 haploid genome equivalents based on an ad genome size of 2 425 mb. clones were stored in 384 384(-) well plates and arrayed into multipl ...200919166501
index selection on seed traits under direct, cytoplasmic and maternal effects in multiple environments.crop seeds are important sources of protein, oil, and carbohydrates for food, animal feeds, and industrial products. recently, much attention has been paid to quality and functional properties of crop seeds. however, seed traits possess some distinct genetic characteristics in comparison with plant traits, which increase the difficulty of genetically improving these traits. in this study, diallel analysis for seed models with genotype by environment interaction (ge) effect was applied to estimat ...200919161944
expression of genes associated with carbohydrate metabolism in cotton stems and roots.cotton (gossypium hirsutum l) is an important crop worldwide that provides fiber for the textile industry. cotton is a perennial plant that stores starch in stems and roots to provide carbohydrates for growth in subsequent seasons. domesticated cotton makes these reserves available to developing seeds which impacts seed yield. the goals of these analyses were to identify genes and physiological pathways that establish cotton stems and roots as physiological sinks and investigate the role these p ...200919161628
overexpression of thellungiella halophila h(+)-ppase (tsvp) in cotton enhances drought stress resistance of plants.an h(+)-ppase gene, tsvp from thellungiella halophila, was transferred into two cotton (gossypium hirsutum) varieties (lumianyan19 and lumianyan 21) and southern and northern blotting analysis showed the foreign gene was integrated into the cotton genome and expressed. the measurement of isolated vacuolar membrane vesicles demonstrated that the transgenic plants had higher v-h(+)-ppase activity compared with wild-type plants (wt). overexpressing tsvp in cotton improved shoot and root growth, and ...200919130078
the ghacs1 gene encodes an acyl-coa synthetase which is essential for normal microsporogenesis in early anther development of cotton.microsporogenesis, associated with the functional expression of many genes, is a highly programmed and regulated process in flowering plants. to elucidate the roles of genes during anther development, two anther-specific cdnas (designated ghacs1 and ghacs2) encoding acyl-coa synthetases (acss) were isolated from a cotton (gossypium hirsutum) flower cdna library. subsequently, the corresponding ghacs1 gene was isolated from a cotton genomic dna library. real-time quantitative rt-pcr and northern ...200918826432
heat stress-induced limitations to reproductive success in gossypium hirsutum.using in vitro systems, numerous authors have cited the sensitivity of pollen tube growth to high temperature as a major cause of low yields for crops with valuable reproductive structures. we investigated the hypothesis that in vivo fertilization efficiency would be negatively affected by heat stress-induced changes in energy reserves and calcium-mediated oxidative status in the pistil. gossypium hirsutum plants exposed to optimal (30/20 degrees c) or high day temperature (38/20 degrees c) cond ...200919656331
conserved mirna analysis in gossypium hirsutum through small rna sequencing.several mirna family and their targets in cotton had been identified by computational methods based on the conserved characterization of mirnas. so far, there are no experiments to validate the existence of mirnas in cotton. in this study, to analyze the mirnas in cotton, a small rna library of sequences from 18 to 26 nt of gossypium hirsutum seedling has been built by high-throughput sequencing. in this library, 34 conserved mirna families were identified by homology search and the mirna sequen ...200919628031
[effects of mepiquat chloride on inorganic elements contents in seeds of transgenic insect-resistant cotton determined by icp-ms].to evaluate the effects of a worldwide used plant growth regulator mepiquat chloride on the nutrition value and safety of seeds of transgenic bt cotton, inorganic element components and contents in seeds of bt cotton (gossypium hirsutum cv. guoxin 6) under field condition were determined using icp-ms. in bt cotton seeds, 54 kinds of inorganic elements were identified by icp-ms, and 5 kinds of major elements, k, p, mg, ca and na, were in range from 138.3 to 13,835.1 microg x g(-1). the contents o ...200919626916
response of the enzymes to nitrogen applications in cotton fiber (gossypium hirsutum l.) and their relationships with fiber strength.to investigate the response of key enzymes to nitrogen (n) rates in cotton fiber and its relationship with fiber strength, experiments were conducted in 2005 and 2006 with cotton cultivars in nanjing. three n rates 0, 240 and 480 kgn/hm(2), signifying optimum and excessive nitrogen application levels were applied. the activities and the gene expressions of the key enzymes were affected by n, and the characteristics of cellulose accumulation and fiber strength changed as the n rate varied. beta-1 ...200919937205
fifty years of the integrated control concept: the role of landscape ecology in ipm in san joaquin valley cotton.in defining the integrated control concept, stern, smith, van den bosch and hagan described 'understanding the ecosystem' as a key underpinning of the concept. in following years, stern and van den bosch continued to refine and expand the role of the ecological landscape. they and their colleagues developed cultural practices that took advantage of this understanding to limit the need of pesticide intervention in cotton in the san joaquin valley during the 1960s and 1970s. research and extension ...200919856340
enrichment of a set of micrornas during the cotton fiber development.cotton (gossypium hirsutum) is one of the most important economic crops and provides excellent fibers for textile manufacture. in addition to its industrial and agricultural importance, the fiber cell (plant trichome) also is a biological model system for exploring gene expression and regulation. small rnas regulate many aspects of plant growth and development. however, whether small rnas are involved in regulation of fiber cell development is unknown.200919788742
molecular and biochemical evidence for phenylpropanoid synthesis and presence of wall-linked phenolics in cotton fibers.the mature cotton (gossypium hirsutum l.) fiber is a single cell with a typically thickened secondary cell wall. the aim of this research was to use molecular, spectroscopic and chemical techniques to investigate the possible occurrence of previously overlooked accumulation of phenolics during secondary cell wall formation in cotton fibers. relative quantitative reverse transcription-polymerase chain reaction analysis showed that ghcad6 and ghcad1 were predominantly expressed among seven gene ho ...200919566641
transcript profiling during fiber development identifies pathways in secondary metabolism and cell wall structure that may contribute to cotton fiber quality.a global gene expression profiling study at different stages of fiber development was undertaken on two cotton species cultivated for fiber, gossypium hirsutum (l.) and g. barbadense (l.). a large proportion of the genome was expressed during both fiber elongation and subsequent secondary cell wall thickening. there was a major shift in abundance of transcripts for gene regulation, cell organization and metabolism between fiber elongation and fiber thickening that was fundamentally similar in bo ...200919520671
identification and molecular mapping of a fusarium wilt resistant gene in upland cotton.fusarium wilt (fw) is one of the most economically damaging cotton diseases worldwide, causing yellowing, wilting, defoliation, vascular tissue damage and ultimately death. identification of molecular markers linked to fw genes is vital to incorporate resistance into elite cotton cultivars. an intraspecific f(2) in gossypium hirsutum l. was developed by crossing with a highly resistant cultivar zhongmiansuo 35 (zms35) and a susceptible cultivar junmian 1 to screen simple sequence repeats (ssrs) ...200919506830
expression and localization of ghh6l, a putative classical arabinogalactan protein in cotton (gossypium hirsutum).arabinogalactan proteins (agps) are a large family of highly glycosylated of hydroxyproline-rich glycoproteins that play important roles in plant growth, development, and signal transduction. a cdna encoding a putative classical agp named ghh6l was isolated from cotton fiber cdna libraries, and the deduced protein contains 17 copies of repetitive motif of x-y-proline-proline-proline (where x is serine or alanine and y is threonine or serine). northern blotting analysis and quantitative rt-pcr re ...200919499153
a synthetic auxin (naa) suppresses secondary wall cellulose synthesis and enhances elongation in cultured cotton fiber.use of a synthetic auxin (naphthalene-1-acetic acid, naa) to start (gossypium hirsutum) ovule/fiber cultures hindered fiber secondary wall cellulose synthesis compared with natural auxin (indole-3-acetic acid, iaa). in contrast, naa promoted fiber elongation and ovule weight gain, which resulted in larger ovule/fiber units. to reach these conclusions, fiber and ovule growth parameters were measured and cell wall characteristics were examined microscopically. the differences in fiber from naa and ...200919479259
a new interspecific, gossypium hirsutum x g. barbadense, ril population: towards a unified consensus linkage map of tetraploid cotton.we report the development of a new interspecific cotton recombinant inbred line (ril) population of 140 lines deriving from an interspecific cross between gossypium hirsutum (gh) and g. barbadense (gb), using the same two parents that have served for the construction of a bc(1) map and for the marker-assisted backcross selection program underway at cirad. two marker systems, microsatellites and aflps, were used. an important feature of the ril population was its marked segregation distortion wit ...200919387609
a specialized outer layer of the primary cell wall joins elongating cotton fibers into tissue-like bundles.cotton (gossypium hirsutum) provides the world's dominant renewable textile fiber, and cotton fiber is valued as a research model because of its extensive elongation and secondary wall thickening. previously, it was assumed that fibers elongated as individual cells. in contrast, observation by cryo-field emission-scanning electron microscopy of cotton fibers developing in situ within the boll demonstrated that fibers elongate within tissue-like bundles. these bundles were entrained by twisting f ...200919369592
interaction of novel dobzhansky-muller type genes for the induction of hybrid lethality between gossypium hirsutum and g. barbadense cv. coastland r4-4.hybrid lethality was identified in interspecific hybrids between two cotton species, gossypium hirsutum and g. barbadense cv. coastland r4-4 (r4-4). genetic analysis indicated that the lethal symptom was controlled by two dominant complementary genes, one from g. hirsutum and another from r4-4. microsatellite mapping identified the location of the causal gene in g. hirsutum as chromosome d8, while the r4-4 gene was placed on chromosome d11. our data indicate that these genes conform to the dobzh ...200919330312
the myb transcription factor ghmyb25 regulates early fibre and trichome development.little is still known about the developmental control of the long seed coat trichomes of cotton (gossypium hirsutum l.). in arabidopsis, leaf trichome initiation is regulated by a group of well-defined transcription factors that includes myb and homeodomain types. many mybs are expressed in fibres, but their roles in fibre development remain unclear. we analysed the function of one myb transcription factor, ghmyb25, identified from transcriptome comparisons between wild-type and fibreless cotton ...200919309462
undamaged cotton plants yield more if their neighbour is damaged: implications for pest management.understanding the compensatory responses of crops to pest damage is important in developing pest thresholds. compensation for pest damage in crops can occur at the plant level, where the architecture, growth dynamics and allocation patterns of damaged plants are altered, allowing them to recover or, at the crop level, where differential damage between plants may alter plant-to-plant interactions. we investigated growth and yield of cotton (gossypium hirsutum l.) following non-uniform manual defo ...200919203400
purification and characterization of cell suspensions peroxidase from cotton (gossypium hirsutum l.).two peroxidases, cpod-i and rpod-ii, have been isolated and purified from cotton cell suspension and their biochemical characteristics studied. rpod-ii from r405-2000, a non-embryogenic cultivar, has higher activity than cpod-i derived from coker 312, which developed an embryogenic structure. the cpod-i and rpod-ii had molecular mass of 39.1 and 64 kda respectively, as determined by sds-page. both enzymes showed high efficiency of interaction with the guaiacol at 25 mm. the optimal ph for cpod-i ...200918649146
tarnished plant bug (hemiptera: miridae) thresholds for cotton before bloom in the midsouth of the united states.insecticide applications to control tarnished plant bug, lygus lineolaris (palisot de beauvois) (hemiptera: miridae), during cotton, gossypium hirsutum l., bud formation are common throughout the midsouth of the united states. cultivation practices and the pest complex have changed since action thresholds were established for this pest. a trial was conducted at 33 locations over 3 yr throughout the midsouth to evaluate tarnished plant bug damage to cotton during the prebloom period. there was no ...200920069839
genome-wide analysis reveals rapid and dynamic changes in mirna and sirna sequence and expression during ovule and fiber development in allotetraploid cotton (gossypium hirsutum l.).cotton fiber development undergoes rapid and dynamic changes in a single cell type, from fiber initiation, elongation, primary and secondary wall biosynthesis, to fiber maturation. previous studies showed that cotton genes encoding putative myb transcription factors and phytohormone responsive factors were induced during early stages of ovule and fiber development. many of these factors are targets of micrornas (mirnas) that mediate target gene regulation by mrna degradation or translational rep ...200919889219
variation in resistance to pyrethroids in helicoverpa armigera from benin republic, west africa.pyrethroid resistance in helicoverpa armigera (hübner) field populations was investigated in benin over several years by using third- and fourth-instar larval topicalbioassays. h. armigera was resistant to pyrethroids tested as cypermethrin, deltamethrin, bifenthrin, and fenvalerate. pretreatment with piperonyl butoxide significantly decreased the ld50 value of cypermethrin and deltamethrin, and the resistance suppression by this synergist effect was observed. no significant decrease in the ld50 ...200919886459
tarnished plant bug (hemiptera: miridae) thresholds and sampling comparisons for flowering cotton in the midsouthern united states.the tarnished plant bug, lygus lineolaris (palisot de beauvois) (hemiptera: miridae), has become the primary target of foliar insecticides in cotton, gossypium hirsutum l., throughout the midsouth over the past several years. this prompted a reevaluation of existing action thresholds for flowering cotton under current production practices and economics. a trial was conducted at 19 locations throughout the midsouth during 2006 and 2007. threshold treatments ranged from a weekly automatic insectic ...200919886447
molecular cloning and localization of a novel cotton annexin gene expressed preferentially during fiber development.annexins constitute a family of multifunction and structurally related proteins. these proteins are ubiquitous in the plant kingdom, and are important calcium-dependent membrane-binding proteins that participate in the polar development of different plant regions such as rhizoids, root caps, and pollen tube tips. in this study, a novel cotton annexin gene (designated as ghfannx) was isolated from a fiber cdna library of cotton (gossypium hirsutum). the full-length cdna of ghfannx comprises an op ...201019885739
characterization of two nadph: cytochrome p450 reductases from cotton (gossypium hirsutum).cytochrome p450 monooxygenases (p450s) are commonly involved in biosynthesis of endogenous compounds and catabolism of xenobiotics, and their activities rely on a partner enzyme, cytochrome p450 reductase (cpr, e.c.1.6.2.4). two cpr cdnas, ghcpr1 and ghcpr2, were isolated from cotton (gossypium hirsutum). they are 71% identical to each other at the amino acid sequence level and belong to the class i and ii of dicotyledonous cprs, respectively. the recombinant enzymes reduced cytochrome c, ferric ...201019883924
long-term effects of land application of class b biosolids on the soil microbial populations, pathogens, and activity.this study evaluated the influence of 20 annual land applications of class b biosolids on the soil microbial community. the potential benefits and hazards of land application were evaluated by analysis of surface soil samples collected following the 20th land application of biosolids. the study was initiated in 1986 at the university of arizona marana agricultural center, 21 miles north of tucson, az. the final application of biosolids was in march 2005, followed by growth of cotton (gossypium h ...201020048328
genotypic differences in thermotolerance are dependent upon prestress capacity for antioxidant protection of the photosynthetic apparatus in gossypium hirsutum.numerous studies have illustrated the need for antioxidant enzymes in acquired photosynthetic thermotolerance, but information on their possible role in promoting innate thermotolerance is lacking. we investigated the hypothesis that genotypic differences in source leaf photosynthetic thermostability would be dependent upon prestress capacity for antioxidant protection of the photosynthetic apparatus in gossypium hirsutum. to test this hypothesis, thermosensitive (cv. st4554) and reportedly ther ...201020002327
a new snp haplotype associated with blue disease resistance gene in cotton (gossypium hirsutum l.).resistance to cotton blue disease (cbd) was evaluated in 364 f(2.3) families of three populations derived from resistant variety 'delta opal'. the cbd resistance in 'delta opal' was controlled by one single dominant gene designated cbd. two simple sequence repeat (ssr) markers were identified as linked to cbd by bulked segregant analysis. cbd resides at the telomere region of chromosome 10. ssr marker dc20027 was 0.75 cm away from cbd. dc20027 marker fragments amplified from 3 diploid species an ...201019960336
proteomic identification of differentially expressed proteins in the ligon lintless mutant of upland cotton (gossypium hirsutum l.).cotton fiber is an ideal model for studying plant cell elongation. to date, the underlying mechanisms controlling fiber elongation remain unclear due to their high complexity. in this study, a comparative proteomic analysis between a short-lint fiber mutant (ligon lintless, li(1)) and its wild-type was performed to identify fiber elongation-related proteins. by 2-de combined with local est database-assisted ms/ms analysis, 81 differentially expressed proteins assigned to different functional cat ...201019954254
programmed cell death during pigment gland formation in gossypium hirsutum leaves.ultrastructural studies have shown that the formation of pigment glands in gossypium hirsutum l. leaves is a lysigenous process, originating from a cluster of cells in the ground meristem. various techniques were used here to investigate whether programmed cell death (pcd) plays a critical role in this developmental process. nuclei of internal cells in the pigment gland-forming tissue were tunel-positive and dapi-negative, suggesting that dna cleavage is an early event and complete dna degradati ...201021040304
[characterization of the damage of spodoptera eridania (cramer) and spodoptera cosmioides (walker) (lepidoptera: noctuidae) to structures of cotton plants].the cotton plant, gossypium hirsutum, hosts various pests that damage different structures. among these pests, spodoptera cosmioides (walker) and spodoptera eridania (cramer) (lepidoptera: noctuidae) are considered important. the objectives of this study were to characterize and to quantify the potential damage of s. eridania and s. cosmioides feeding on different structures of cotton plants. for this purpose, newly-hatched larvae were reared on the following plant parts: leaf and flower bud; le ...201020878002
association of aflp and ssr markers with agronomic and fibre quality traits in gossypium hirsutum l.molecular markers linked to qtl contributing to agronomic and fibre quality traits would be useful for cotton improvement. we have attempted to tag yield and fibre quality traits with aflp and ssr markers using f(2) and f(3) populations of a cross between two gossypium hirsutum varieties, ps56-4 and rs2013. out of 50 aflp primer combinations and 177 ssr primer pairs tested, 32 aflp and four ssr primers were chosen for genotyping f(2) individuals. marker-trait associations were studied for eight ...201020861566
two cotton cys2/his2-type zinc-finger proteins, ghdi19-1 and ghdi19-2, are involved in plant response to salt/drought stress and abscisic acid signaling.cotton (gossypium hirsutum) often encounters abiotic stress such as drought and high salinity during its development, and its productivity is significantly limited by those adverse factors. to investigate the molecular adaptation mechanisms of this plant species to abiotic stress, we identified two genes encoding di19-like cys2/his2 zinc-finger proteins in cotton. gfp fluorescence assay demonstrated that ghdi19-1 and ghdi19-2 are two nuclear-localized proteins. quantitative rt-pcr and northern b ...201020852918
near-isogenic cotton germplasm lines that differ in fiber-bundle strength have temporal differences in fiber gene expression patterns as revealed by comparative high-throughput profiling.gene expression profiles of developing cotton (gossypium hirsutum l.) fibers from two near-isogenic lines (nils) that differ in fiber-bundle strength, short-fiber content, and in fewer than two genetic loci were compared using an oligonucleotide microarray. fiber gene expression was compared at five time points spanning fiber elongation and secondary cell wall (scw) biosynthesis. fiber samples were collected from field plots in a randomized, complete block design, with three spatially distinct b ...201020087569
cotton chromosome substitution lines crossed with cultivars: genetic model evaluation and seed trait analyses.seed from upland cotton, gossypium hirsutum l., provides a desirable and important nutrition profile. in this study, several seed traits (protein content, oil content, seed hull fiber content, seed index, seed volume, embryo percentage) for f(3) hybrids of 13 cotton chromosome substitution lines crossed with five elite cultivars over four environments were evaluated. oil and protein were expressed both as percentage of total seed weight and as an index which is the grams of product/100 seeds. an ...201020087566
development and population growth of lygus hesperus on selected weed hosts, artificial diet and cotton in the laboratory.laboratory studies were conducted to obtain basic biological information of lygus hesperus knight (hemiptera: miridae) on alfalfa (medicago sativa l.), pigweed (amaranthus palmeri s. wats.), russian thistle (salsola iberica sennen and pau), green bean (phaseolus vulgaris l.), artificial diet, and cotton (gossypium hirsutum l.) square and cotton boll at 27 degrees c. the nymphal developmental duration was 28.8 d on cotton bolls, nearly twice longer, compared with approximately 15 d in other hosts ...201021309220
transcriptome profiling of early developing cotton fiber by deep-sequencing reveals significantly differential expression of genes in a fuzzless/lintless mutant.cotton fiber as a single-celled trichome is a biological model system for studying cell differentiation and elongation. however, the complexity of its gene expression and regulatory mechanism allows only marginal progress. here, we report the high-throughput tag-sequencing (tag-seq) analysis using solexa genome analyzer platform on transcriptome of -2 to 1 (fiber initiation, stage i) and 2-8 (fiber elongation, stage ii) days post anthesis (dpa) cotton (gossypium hirsutum) ovules (wild type: wt; ...201020828606
the history and disposition of transposable elements in polyploid gossypium.transposable elements (tes) are a major component of plant genomes. it is of particular interest to explore the potential activation of te proliferation, especially in hybrids and polyploids, which often are associated with rapid genomic and epigenetic restructuring. here we explore the consequences of genomic merger and doubling on copia and gypsy-like gorge3 long terminal repeat (ltr) retrotransposons as well as on non-ltr long interspersed nuclear elements (lines) in allotetraploid cotton, go ...201020725147
comparisons of early transcriptome responses to low-oxygen environments in three dicotyledonous plant species.waterlogging is a serious impediment to crop productivity worldwide which acts to reduce oxygen levels in the rhizosphere due to the low diffusion rate of molecular oxygen in water. plants respond to low oxygen through rapid and specific changes at both the transcriptional and translational levels. transcriptional changes to low-oxygen (hypoxia) stress have been studied in a number of plant species using whole genome microarrays. using transcriptome data from root tissue from early time points ( ...201020724824
identification of the family of aquaporin genes and their expression in upland cotton (gossypium hirsutum l.).cotton (gossypium spp.) is produced in over 30 countries and represents the most important natural fiber in the world. one of the primary factors affecting both the quantity and quality of cotton production is water. a major facilitator of water movement through cell membranes of cotton and other plants are the aquaporin proteins. aquaporin proteins are present as diverse forms in plants, where they function as transport systems for water and other small molecules. the plant aquaporins belong to ...201020626869
meta-analysis of cotton fiber quality qtls across diverse environments in a gossypium hirsutum x g. barbadense ril population.cotton fibers (produced by gossypium species) are the premier natural fibers for textile production. the two tetraploid species, g. barbadense (gb) and g. hirsutum (gh), differ significantly in their fiber properties, the former having much longer, finer and stronger fibers that are highly prized. a better understanding of the genetics and underlying biological causes of these differences will aid further improvement of cotton quality through breeding and biotechnology. we evaluated an inter-spe ...201020584292
overexpression of a profilin (ghpfn2) promotes the progression of developmental phases in cotton fibers.cotton fiber development at the stages of elongation and secondary wall synthesis determines the traits of fiber length and strength. to date, the mechanisms controlling the progression of these two phases remain elusive. in this work, the function of a fiber-preferential actin-binding protein (ghpfn2) was characterized by cytological and molecular studies on the fibers of transgenic green-colored cotton (gossypium hirsutum) through three successive generations. overexpression of ghpfn2 caused p ...201020558432
[determination of protein and gossypol content in cotton kernel powder with near infrared reflectance spectroscopy].near-infrared reflectance spectroscopy (nirs) was used as a rapid and nondestructive method to determine the protein content and gossypol content in cotton kernel powder samples, using 49 upland cotton (gossypium hirsutum l.) germplasms and 188 recombinant inbred lines (rils). the cottonseed samples harvested from the upland cotton germplasms and rils grown in different cotton growing regions in different years were analyzed chemically for protein and gossypol contents, as well as scanned in the ...201020496676
cold acclimation and low temperature resistance in cotton: gossypium hirsutum phospholipase dalpha isoforms are differentially regulated by temperature and light.phospholipase dalpha (pldalpha) was isolated from cultivated cotton (gossypium hirsutum) and characterized. two pldalpha genes were identified in the allotetraploid genome of g. hirsutum, derived from its diploid progenitors, g. raimondii and g. arboreum. the genes contained three exons and two introns. the translated products shared a 98.6% homology and were designated as grpldalpha and gapldalpha. their orfs encoded a polypeptide of 807 amino acids with a predicted molecular mass of 91.6 kda s ...201020478966
identification and evaluation of new reference genes in gossypium hirsutum for accurate normalization of real-time quantitative rt-pcr data.normalizing through reference genes, or housekeeping genes, can make more accurate and reliable results from reverse transcription real-time quantitative polymerase chain reaction (qpcr). recent studies have shown that no single housekeeping gene is universal for all experiments. thus, suitable reference genes should be the first step of any qpcr analysis. only a few studies on the identification of housekeeping gene have been carried on plants. therefore qpcr studies on important crops such as ...201020302670
characterization of two cotton (gossypium hirsutum l) invertase genes.two cotton vacuolar-invertase genes were identified and sequenced. both genes had seven exons, including an unusually small second exon typical of acid invertases. these genes encode peptides with many features shared by acid invertases from other species including, leader sequences that probably target the peptide to the vacuole, active site motifs and substrate binding motifs. expression analyses indicated that one of the genes was expressed in roots during the starch filling stage of developm ...201020300865
effect of h2o2 on fiber initiation using fiber retardation initiation mutants in cotton (gossypium hirsutum).single-celled fibers initiate at anthesis from cotton seed epidermal cells of normal developmental cotton cultivars; however, fiber initiation is retarded in some cotton fiber mutants. in this study, the relationship between genes associated with fiber initiation retardation and fiber initiation development was investigated using three cotton fiber developmental mutants: recessive naked seed n2; dominant naked seed n1; and xinxiangxiaoji linted-fuzzless mutant (xinflm); with genetic standard lin ...201019931935
global gene expression responses to waterlogging in roots and leaves of cotton (gossypium hirsutum l.).waterlogging stress causes yield reduction in cotton (gossypium hirsutum l.). a major component of waterlogging stress is the lack of oxygen available to submerged tissues. while changes in expressed protein, gene transcription and metabolite levels have been studied in response to low oxygen stress, little research has been done on molecular responses to waterlogging in cotton. we assessed cotton growth responses to waterlogging and assayed global gene transcription responses in root and leaf c ...201019923201
molecular cloning and characterization of five genes encoding pentatricopeptide repeat proteins from upland cotton (gossypium hirsutum l.).the pentatricopeptide repeat (ppr) protein family is one of the largest and most complex families in plants. these proteins contain multiple 35-amino acid repeats that are proposed to form a super helix capable of binding rna. ppr proteins have been implicated in many crucial functions broadly involving organelle biogenesis and plant development. in this study, we identified many genes encoding ppr protein in upland cotton through an extensive survey of the database of gossypium hirsutum. furthe ...201019578975
fate and efficacy of metolachlor granular and emulsifiable concentrate formulations in a conservation tillage system.use of genetically modified cultivars resistant to the herbicide glyphosate (n-phosphonomethylglycine) is strongly associated with conservation-tillage (cst) management for maize ( zea mays l.), soybean ( glycine max l.), and cotton ( gossypium hirsutum l.) cultivation. due to the emergence of glyphosate-resistant weed biotypes, alternate weed management practices are needed to sustain cst use. this work focused on metolachlor use (2-chloro-n-(2-ethyl-6-methylphenyl)-n-(2-methoxy-1-methylethyl)a ...201020836497
inheritance of resistance to meloidoygne incognita in primitive cotton accessions from mexico.few sources of resistance to root-knot nematodes (meloidogyne incognita) in upland cotton (gossypium hirsutum) have been utilized to develop resistant cultivars, making this resistance vulnerable to virulence in the pathogen population. the objectives of this study were to determine the inheritance of resistance in five primitive accessions of g. hirsutum (tx1174, tx1440, tx2076, tx2079, and tx2107) and to determine allelic relations with the genes for resistance in the genotypes clevewilt-6 (cw ...201022736869
geostatistical modeling of the spatial variability and risk areas of southern root-knot nematodes in relation to soil properties.identifying the spatial variability and risk areas for southern root-knot nematode [meloidogyne incognita (kofoid & white) chitwood] (rkn) is key for site-specific management (ssm) of cotton (gossypium hirsutum l.) fields. the objectives of this study were to: (i) determine the soil properties that influence rkn occurrence at different scales; and (ii) delineate risk areas of rkn by indicator kriging. the study site was a cotton field located in the southeastern coastal plain region of the usa. ...201020717481
fine mapping qmi-c11 a major qtl controlling root-knot nematodes resistance in upland cotton.the identification and utilization of a high-level of host plant resistance is the most effective and economical approach to control root-knot nematode (meloidogyne incognita). in an earlier study, we identified a major quantitative trait locus (qtl) for resistance to root-knot nematode in the m-120 rnr upland cotton line (gossypium hirsutum l.) of the auburn 623 rnr source. the qtl is located in a 12.9-cm interval flanked by the two ssr markers cir069 and cir316 on the distal segment of chromos ...201020680610
ssr markers closely associated with genes for resistance to root-knot nematode on chromosomes 11 and 14 of upland cotton.molecular markers closely linked to genes that confer a high level of resistance to root-knot nematode (rkn) [meloidogyne incognita (kofoid & white) chitwood] in cotton (gossypium hirsutum l.) germplasm derived from auburn 623 rnr would greatly facilitate cotton breeding programs. our objectives were to identify simple sequence repeat (ssr) markers linked to rkn resistance quantitative trait loci (qtl) and map these markers to specific chromosomes. we developed three recombinant inbred line (ril ...201020607210
effect of mowing cotton stalks and preventing plant re-growth on post-harvest reproduction of meloidogyne incognita.the southern root-knot nematode (meloidogyne incognita) is a major parasite of cotton in the u.s., and management tactics for this nematode attempt to minimize population levels. we compared three post-harvest practices for their ability to reduce nematode population levels in the field, thereby reducing initial nematode population for the next year's crop. the three practices tested were: 1) chemical defoliation before harvest plus cutting cotton stalks after harvest, 2) chemical defoliation pl ...201022736845
a new cotton sdr family gene encodes a polypeptide possessing aldehyde reductase and 3-ketoacyl-coa reductase activities.to understand regulatory mechanisms of cotton fiber development, microarray analysis has been performed for upland cotton (gossypium hirsutum). based on this, a cdna (ghkcr3) encoding a polypeptide belonging to short-chain alcohol dehydrogenase/reductase family was isolated and cloned. it contains an open reading frame of 987 bp encoding a polypeptide of 328 amino acid residues. following its overexpression in bacterial cells, the purified recombinant protein specifically uses nadph to reduce a ...201020370610
genetic transformation of cotton with a harpin-encoding gene hpaxoo confers an enhanced defense response against different pathogens through a priming mechanism.the soil-borne fungal pathogen verticillium dahliae kleb causes verticillium wilt in a wide range of crops including cotton (gossypium hirsutum). to date, most upland cotton varieties are susceptible to v. dahliae and the breeding for cotton varieties with the resistance to verticillium wilt has not been successful.201020398293
development of sampling plans for cotton bolls injured by stink bugs (hemiptera: pentatomidae).cotton, gossypium hirsutum l., bolls were sampled in commercial fields for stink bug (hemiptera: pentatomidae) injury during 2007 and 2008 in south carolina and georgia. across both years of this study, boll-injury percentages averaged 14.8 +/- 0.3 (sem). at average boll injury treatment levels of 10, 20, 30, and 50%, the percentage of samples with at least one injured boll was 82, 97, 100, and 100%, respectively. percentage of field-sampling date combinations with average injury < 10, 20, 30, a ...201020429470
bcg vaccination in the cotton rat (sigmodon hispidus) infected by the pulmonary route with virulent mycobacterium tuberculosis.to evaluate the usefulness of the american cotton rat (sigmodon hispidus) in the evaluation of vaccine-induced resistance, we infected bcg-vaccinated and non-vaccinated cotton rats with mycobacterium tuberculosis (h37rv) via the respiratory route. lung histopathology of these animals showed loose, disorganized granulomas which were non-necrotic up to 8 weeks post-infection. moreover, we were not able to detect a dth response after intradermal injection with ppd antigen. prior bcg vaccination sig ...201020451457
influence of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina.three studies were conducted to determine the effect of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina. in all three studies, samples of wireworm populations were taken from the soil by using oat, avena sativa l., baits. treatments were defined by the previous year's crop and were chosen to reflect common crop rotations in the region. across all three studies, eight wireworm species were recovered from the baits: conoderus amplicollis (gyllen ...201021309229
characteristics of resistance to bacillus thuringiensis toxin cry2ab in a strain of helicoverpa punctigera (lepidoptera: noctuidae) isolated from a field population.in 1996, the australian cotton industry adopted ingard that expresses the bacillus thuringiensis (bt) toxin gene cry1ac and was planted at a cap of 30%. in 2004-2005, bollgard ii, which expresses cry1ac and cry2ab, replaced ingard in australia, and subsequently has made up >80% of the area planted to cotton, gossypium hirsutum l. the australian target species helicoverpa armigera (hübner) and helicoverpa punctigera (wallengren) are innately moderately tolerant to bt toxins, but the absence of a ...201021309238
vip3aa tolerance response of helicoverpa armigera populations from a cry1ac cotton planting region.transgenic cotton, gossypium hirsutum l., that expresses the bacillus thuringiensis (bt) cry1ac toxin, holds great promise in controlling target insect pests. evolution of resistance by target pests is the primary threat to the continued efficacy of bt cotton. to thwart pest resistance evolution, a transgenic cotton culitvar that produces two different bt toxins, cry1ac and vip3a genes, was proposed as a successor of cry1ac cotton. this article reports on levels of vip3aa tolerance in helicoverp ...201021309241
relationship between external stink bug (hemiptera: pentatomidae) boll-feeding symptoms and internal boll damage with respect to cotton lint gin-out and fiber quality.cotton, gossypium hirsutum l., bolls from 17 field locations in northeastern north carolina and southeastern virginia, having 20% or greater internal boll damage, were studied to determine the relationship between external feeding symptoms and internal damage caused by stink bug (hemiptera: pentatomidae) feeding. in 2006 and 2007, two cohorts of 100 bolls each were sampled at all field locations. the first cohort was removed as bolls reached approximately quarter size in diameter (2.4 cm). exter ...201021309249
oviposition and development of the tarnished plant bug (heteroptera: miridae) on field maize.reduced insecticide use in cotton, gossypium hirsutum l., as a consequence of the boll weevil eradication program and the broad adoption of bt cotton, have helped make the tarnished plant bug, lygus lineolaris (palisot de beauvois), a consistent pest of cotton each year in the mid-south. maize, zea mays l., has been implicated as having a role in the season-long dynamics of tarnished plant bug infestations in cotton. to date, no published information exists describing the quality of maize as a ...201022127158
clustering, haplotype diversity and locations of mic-3: a unique root-specific defense-related gene family in upland cotton (gossypium hirsutum l.).mic-3 is a recently identified gene family shown to exhibit increased root-specific expression following nematode infection of cotton plants that are resistant to root-knot nematode. here, we cloned and sequenced mic-3 genes from selected diploid and tetraploid cotton species to reveal sequence differences at the molecular level and identify chromosomal locations of mic-3 genes in gossypium species. detailed sequence analysis and phylogenetic clustering of mic-3 genes indicated the presence of m ...201019862497
impact of rotylenchulus reniformis on cotton yield as affected by soil texture and irrigation.the effects of soil type, irrigation, and population density of rotylenchulus reniformis on cotton were evaluated in a two-year microplot experiment. six soil types, fuquay sand, norfolk sandy loam, portsmouth loamy sand, muck, cecil sandy loam, and cecil sandy clay, were arranged in randomized complete blocks with five replications. each block had numerous plots previously inoculated with r. reniformis and two or more noninoculated microplots per soil type, one half of which were irrigated in e ...201022736865
natural migration of rotylenchulus reniformis in a no-till cotton system.rotylenchulus reniformis is the most damaging nematode pathogen of cotton in alabama. it is easily introduced into cotton fields via contaminated equipment and, when present, is difficult and costly to control. a trial to monitor the natural migration of r. reniformis from an initial point of origin was established in 2007 and studied over two growing seasons in both irrigated and non-irrigated no-till cotton production systems. vermiform females, juveniles and males reached a horizontal distanc ...201022736863
molecular and morphological characterization and biological control capabilities of a pasteuria ssp. parasitizing rotylenchulus reniformis, the reniform nematode.rotylenchulus reniformis is one of 10 described species of reniform nematodes and is considered the most economically significant pest within the genus, parasitizing a variety of important agricultural crops. rotylenchulus reniformis collected from cotton fields in the southeastern us were observed to have the nematode parasitic bacterium pasteuria attached to their cuticles. challenge with a pasteuria-specific monoclonal antibody in live immuno-fluorescent assay (ifa) confirmed the discovery of ...201022736858
classification of rotylenchulus reniformis numbers in cotton using remotely sensed hyperspectral data on self-organizing maps.rotylenchulus reniformis is one of the major nematode pests capable of reducing cotton yields by more than 60%, causing estimated losses that may exceed millions of dollars u.s. therefore, early detection of nematode numbers is necessary to reduce these losses. this study investigates the feasibility of using remotely sensed hyperspectral data (reflectances) of cotton plants affected with different nematode population numbers with self-organizing maps (som) in correlating and classifying nematod ...201022736855
remotely sensing arthropod and nutrient stressed plants: a case study with nitrogen and cotton aphid (hemiptera: aphididae).remote sensing can be used in combination with ground sampling to detect aphid- (aphis gossypii glover) infested cotton (gossypium hirsutum l.). changes in wavelengths in the near-infrared (nir) have proven useful for such detection, but these changes can be confused with other factors stressing plants, such as water deficiency and nutrient status. this study was designed to test the utility of this technology to distinguish between two factors stressing plants: nitrogen deficiency and aphids. f ...201022127176
effect of reproductive mode on host plant utilization of melon aphid (hemiptera: aphididae).variation in the reproductive mode of melon aphid aphis gossypii glover occurred on the large geographic scale, but the performance of different reproductive modes to use host plant is poorly understood. life tables of melon aphid population that undergo the anholocyclic, androcyclic, and intermediate reproductive mode were conducted on different host plants. the results showed that the anholocyclic and androcyclic strains could become adults and produce offspring on cotton gossypium hirsutum l. ...201022182553
a draft physical map of a d-genome cotton species (gossypium raimondii).genetically anchored physical maps of large eukaryotic genomes have proven useful both for their intrinsic merit and as an adjunct to genome sequencing. cultivated tetraploid cottons, gossypium hirsutum and g. barbadense, share a common ancestor formed by a merger of the a and d genomes about 1-2 million years ago. toward the long-term goal of characterizing the spectrum of diversity among cotton genomes, the worldwide cotton community has prioritized the d genome progenitor gossypium raimondii ...201020569427
apyrase (nucleoside triphosphate-diphosphohydrolase) and extracellular nucleotides regulate cotton fiber elongation in cultured ovules.ectoapyrase enzymes remove the terminal phosphate from extracellular nucleoside tri- and diphosphates. in arabidopsis (arabidopsis thaliana), two ectoapyrases, atapy1 and atapy2, have been implicated as key modulators of growth. in fibers of cotton (gossypium hirsutum), transcript levels for ghapy1 and ghapy2, two closely related ectoapyrases that have high sequence similarity to atapy1 and atapy2, are up-regulated when fibers enter their rapid growth phase. in an ovule culture system, fibers re ...201020018604
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