Publications

identification and quantification of ureaplasmas colonizing the respiratory tract and assessment of their role in the development of chronic lung disease in preterm infants.	the role of ureaplasma urealyticum in the development of chronic lung disease (cld) in preterm infants continues to be disputed. recently u. urealyticum has been found to consist of two species, u. urealyticum and ureaplasma parvum, a finding that has not been considered in previous studies of cld. this study examined the possible relationships between development of cld and respiratory colonization by these newly redefined species, their concentrations in lower respiratory secretions and the ef ...	2001	11734763
a clinical study on the association of trichomonas vaginalis and mycoplasma hominis infections in women attending a sexually transmitted disease (std) outpatient clinic.	swabs from the posterior vaginal fornix were obtained from 804 consecutive female patients visiting a large dutch sexually transmitted diseases (std) outpatient clinic. a detailed clinical history was obtained and complaints concerning the lower genital tract, such as vaginal discharge or vulval and vaginal irritation, were recorded. patients were examined and the presence of non-physiological vaginal secretions was established by speculum examination. the swabs were monitored for bacterial vagi ...	2001	11750218
production of pro- and anti-inflammatory cytokines of human placental trophoblasts in response to pathogenic bacteria.	we studied the production of cytokines in purified cultures of human term trophoblasts in the presence of pathogenic strains of escherichia coli, bacteroides fragilis, mycoplasma hominis, staphylococcus aureus, and streptococcus agalactiae, which have been identified in intrauterine infections.	2001	11750868
mycoplasma hominis and trichomonas vaginalis symbiosis: multiplicity of infection and transmissibility of m. hominis to human cells.	we recently reported that most trichomonas vaginalis isolates cultured in vitro are infected by mycoplasma hominis. in this work, we have characterized some aspects of the relationships between the two microorganisms. pcr, cultivation, and immunological methods revealed that the number of m. hominis organisms carried by t. vaginalis in culture varied from isolate to isolate, suggesting a specific multiplicity of infection. moreover, infected t. vaginalis isolates were able to pass bacteria not o ...	2001	11271423
[cytotoxic activity of melittin expressed by recombinant vectors in acholeplasma laidlawii and mycoplasma hominis cells].	recombinant plasmids containing the mellitin gene under the control of the lac and the tetm gene promoters were used for studying cytotoxic activity of mellitin in cells of mollicutes (mycoplasmas) and escherichia coli. after transformation of acholeplasma laidlawii and mycoplasma hominis cells with recombinant plasmid dnas by electroporation, cell growth was suppressed. the expression of the mellitin gene in a. laidlawii and m. hominis cells was demonstrated using the reverse transcription poly ...	2001	11234424
detection of cervical immunoglobulin a in normal pregnancy.	the aim of our study was to evaluate a feasible method to quantify the immunoglobulin a concentration in the cervical mucus of women with a normal singleton pregnancy. in 60 immunologic healthy pregnant women cervical mucus samples were taken at a random time in pregnancy using an absorbing cylindrical cotton-swab stick. in this cervical mucus immunoglobulin a concentration was measured by radial immunodiffusion. a vaginal bacterial swab was taken from each woman. concentration of immunoglobulin ...	2001	11234615
a cluster of mycoplasma hominis infection in heart-lung transplantation.		2001	11250419
is mycoplasma hominis a vaginal pathogen?	to evaluate the role of mycoplasma hominis as a vaginal pathogen.	2001	11158693
[classification of urogenital tract infection in 518 cases and drug sensitivity analysis].	in 518 cases with urogenital tract infection detected with mycoplasma identification susceptibility testing reagent box, 308 cases (59.4%) were infected by ureaplasma urealyticum (uu), 21 cases (4.1%) were infected by mycoplasma hominis (mh), and 189 cases (36.5%) were infected by both uu and mh. drug sensitivity to the macrolides (erythromycin, roxithromycin, josamycin, azithromycin), the tetracyclines (doxycycline, minocin), and the quinolines (ofloxacin, ciprofloxacin) were detected at the sa ...	2001	12536620
polymorphism of immune response genes as a factor for predisposition to development of diseases.	the association of particular allelic variants of genes coding proteins, such as transporter associated with processing (tap) and heat shock protein (hsp), involved in endocellular processing of endogenous antigens, with synovium pathology accompanied with urogenital infection was observed. the particular alleles of tap1 and tap2 genes appeared to be the factor of predisposition or resistance for chlamidia trachomatis but not for mycoplasma hominis. at the same time, the relative risk for c. tra ...	2001	12687213
epidemiology of sexually transmitted diseases in río cuarto, argentina.	sexually transmitted diseases (std) are a group of transmittable diseases acquired fundamentally through sexual contact. std are a social problem resulting from demographic explosion and changes in sexual conduct, which affects teenagers and adults of all socioeconomic strata. the goal of this work was to establish the actual state of the different std within the studied population. samples of vaginal fluids, endocervical materials and urethral exudates taken from 2,630 patients during five year ...	2001	17061502
demographic, clinical and microbiological characteristics of maternity patients: a canadian clinical cohort study.	objective: to determine the demographic, clinical and microbiological characteristics of a representative canadian obstetrical population. design: a one-year cohort study of all maternity patients who were followed to delivery, using detailed patient questionnaires containing more than 60 demographic and clinical variables, and three microbiological evaluations during gestation - first trimester, 26 to 30 weeks, and labour and delivery. outcome measurements included birth weight and gestational ...	2002	18159407
recombination in mycoplasma hominis.	mycoplasma hominis has been previously described as a heterogeneous species, and in the present study intraspecies diversity of 20 m. hominis isolates from different individuals was analyzed using parts of the unlinked gyrase b (gyrb), elongation factor tu (tuf), sralpha homolog (ftsy), hitb-hitl, excinuclease abc subunit a (uvra) and glyceraldehyde-3-phosphate dehydrogenase (gap) genes. the level of variability of these m. hominis genes was low compared with the housekeeping genes from helicoba ...	2002	12798006
in vitro susceptibility of mycoplasma hominis clinical isolates to tetracyclines, quinolones and macrolides.	we tested the in vitro activity of levofloxacin, ciprofloxacin, doxycycline, tetracycline, erythromycin, roxithromycin, clarithromycin and azithromycin against 110 clinical isolates of mycoplasma hominis. the minimal inhibitory concentrations (mics) were determined with the etest. the minimal concentrations at which 90% of the isolates were inhibited (mic(90)) were 0.064 microg/ml doxycycline and 0.19 microg/ml tetracycline. in 9 isolates (8.1%), the mic for doxycycline was 4-12 microg/ml. these ...	2002	12543541
vaginal bacterial flora activates rat peritoneal mast cells.	sixteen strains of physiological and pathological vaginal bacteria were tested for their ability to secrete histamine from rat peritoneal mast cells in vitro. we noticed that mycoplasma hominis-induced histamine release was very high (up to 53.6%). the stimulation of rat mast cells with staphylococccus cohnii, staphylococcus coagulase(-) (two strains), ureaplasma urealyticum, peptostreptococcus spp., bacteroides capillosus, staphylococcus aureus and streptococcus agalactiae resulted in lower but ...	2002	12575924
mycoplasma genitalium is not associated with adverse outcomes of pregnancy in guinea-bissau.	to evaluate the impact of mycoplasma genitalium on the outcome of pregnancy.	2002	12181470
identification and functional mapping of the mycoplasma fermentans p29 adhesin.	initial adherence interactions between mycoplasmas and mammalian cells are important for host colonization and may contribute to subsequent pathogenic processes. despite significant progress toward understanding the role of specialized, complex tip structures in the adherence of some mycoplasmas, particularly those that infect humans, less is known about adhesins through which other mycoplasmas of this host bind to diverse cell types, even though simpler surface components are likely to be invol ...	2002	12183538
pelvic inflammatory disease in the people's republic of china: aetiology and management.	to study the causes of pelvic inflammatory disease (pid) in shenyang, northeastern china, and to assess the efficacy of the syndromic management flowchart for pid recommended by world health organization (who).	2002	12194742
safety and tolerability of the new contraceptive sponge protectaid.	this study was undertaken to assess the efficacy, safety and acceptability of two different-dose regimens of the protectaid contraceptive sponge.	2002	12201327
characterization of p40, a cytadhesin of mycoplasma agalactiae.	an immunodominant protein, p40, of mycoplasma agalactiae was analyzed genetically and functionally. the gene encoding p40 was cloned from type strain pg2, sequenced, submitted to point mutagenesis in order to convert mycoplasma-specific tga(trp) codon to the universal tgg(trp) codon, and subsequently expressed in escherichia coli. nucleotide sequence-derived amino acid sequence comparisons revealed a similarity of p40 to the adhesin p50 of mycoplasma hominis and to protein p89 of spiroplasma cit ...	2002	12228289
mutations in 23s rrna account for intrinsic resistance to macrolides in mycoplasma hominis and mycoplasma fermentans and for acquired resistance to macrolides in m. hominis.	the mechanisms of intrinsic resistance of mycoplasma hominis to 14- and 15-membered macrolides were investigated in comparison with those of m. pneumoniae, which is naturally susceptible to macrolides. radiolabeled erythromycin was not accumulated by m. hominis pg21, but addition of an abc transporter inhibitor increased the level of erythromycin uptake more than two times, suggesting the existence of an active efflux process. the affinity of [(14)c]erythromycin to ribosomes isolated from m. hom ...	2002	12234836
[allelic polymorphism of the tem(m) determinant in mycoplasma hominis and ureaplasma urealyticum clinical isolates resistant to tetracyclines].	of the 130 clinical isolates of mycoplasma hominis from patients with nonspecific inflammatory diseases of the urogenital tract (ugt), approximately 10% contained the tet(m) gene after the course of treatment with tetracyclines. this gene was found in nine (25%) of the 36 ureaplasma urealyticum clinical isolates. the nucleotide sequence of 13 tet(m) genes in tcr clinical isolates of m. hominis and five genes in u. urealyticum tcr clinical isolates was determined. a comparison of nucleotide seque ...	2002	12500671
mycoplasma hominis scalp abscess in the newborn.	scalp abscess in the newborn is usually a complication of fetal scalp monitoring and is typically polymicrobial, reflecting maternal vaginal flora. we present a newborn with no apparent scalp trauma who developed scalp abscess that yielded pure growth of mycoplasma hominis. the clue to diagnosis was the growth of pinpoint translucent colonies on blood agar that were gram stain-negative. abscess drainage and systemic clindamycin were curative.	2002	12506953
[the microorganisms associated with bacterial vaginosis as a cause of tubal sterility].	bacterial vaginosis is one of the most widespread disturbances of the normal state of the vagina of women of reproductive and menopausal age. this condition is highly disregarded due to its moderate and not always clear clinical manifestation. the disturbed proportion of microorganisms in vagina is shown in the strong decrease or lack of lactobacilli and a spread of associated with bacterial vaginosis microorganisms. g. vaginalis, mobiluncis spp., bacteroides spp. (without b. fragilis), peptostr ...	2002	12516260
[application of different methods in the diagnosis of respiratory mycoplasmosis].	a complex of methods for the detection of mycoplasma pneumoniae and mycoplasma hominis in children and adults with respiratory diseases (acute, chronic and obstructive bronchitis, pneumonia, recurring croup, bronchial asthma), as well as in children frequently having acute respiratory diseases, has been worked out and tested. both infective agents are frequently detected in the above mentioned pathological processes as monoinfection or mixed infection. mycoplasma antigens are capable of prolonge ...	2002	12525003
vaginal lactobacilli and preterm birth.	to assess the relationship between the absence of vaginal lactobacilli and preterm birth at < 33 weeks of gestation.	2002	12530101
have ureaplasma urealyticum and mycoplasma hominis infections any significant effect on women fertility?	ureaplasma urealyticum and mycoplasma hominis are known as sexually transmitted agents. u. urealyticum and m. hominis jeopardize male fertility. however, it is unclear whether these infections significantly contribute to female infertility. in this controlled-study we aimed to establish whether m. hominis and u. urealyticum are risk factors for female fertility and prevalence of infection from these agents in patients attending our infertility clinic. total 96 married women enrolled in this pros ...	2002	12754428
preterm labour--is mycoplasma hominis involved?	to assess whether mycoplasma hominis is associated with preterm labour in primigravidae and multigravidae with previous midtrimester abortion or preterm labour.	2002	12040954
[cervicovaginal infection as a risk factor for premature labor].	to identify the possible association between cervicovaginal infections (cvi) and preterm delivery.	2002	12050955
characterization of the mycoplasma hominis ftsz gene and its sequence variability in mycoplasma clinical isolates.	we cloned and sequenced mycoplasma hominis chromosomal fragment containing ftsz gene. the wild-type expression of the gene was shown at rna level by reverse transcription followed by pcr amplification. we revealed that m. hominis ftsz had a comparatively low similarity to proteins of mycoplasma genitalium and mycoplasma pneumoniae. after full ftsz gene sequencing for 14 clinical isolates of m. hominis, single-nucleotide substitutions were found in 21 positions, 6 of them being common for almost ...	2002	12054578
[antigenic variation in mycoplasmas].		2002	12102047
[cervico-vaginal infections. study of a population in the turin area].	aim of this study is to determine the prevalence, in the turin area, of the pathogens chiefly involved in the genesis of the most common infections of the female genitalia.	2002	12114863
[development of fluoroquinolone (ciprofloxacin) resistance in mycoplasma hominis in the presence of hela cells].	the effect of cocultivation of eukaryotic hela cells and mycoplasma hominis mycoplasma on the resistance of the latter to fluoroquinolones (ciprofloxacin) was examined. it was shown that cocultivation of the m. homonis and hela cells during 24 h with subsequent addition of ciprofloxacin resulted in an increase of the mircoplasma resistance to this antimicrobial agent. in the m. hominis cells cultivated in the presence of hela cells and the increasing concentration of ciprofloxacin mutations in t ...	2002	12174584
douching in relation to bacterial vaginosis, lactobacilli, and facultative bacteria in the vagina.	to study how frequency, recentness, and reason for douching impact bacterial vaginosis-related vaginal microflora and the occurrence of cervical pathogens. douching has been linked to bacterial vaginosis as well as to chlamydial cervicitis in some, but not all, studies.	2002	12383547
in vitro antimycoplasmal activity of oleuropein.	the activity of oleuropein, a phenolic glycoside contained in olive oil, was investigated in vitro against mycoplasma hominis, mycoplasma fermentans, mycoplasma pneumoniae and mycoplasma pirum. oleuropein inhibited mycoplasmas at concentrations from 20 to 320 mg/l. the mics of oleuropein to m. pneumoniae, m. pirum, m. hominis and m. fermentans were 160, 320, 20 and 20 mg/l, respectively.	2002	12385687
probiotics--a therapy in search of a disease.		2002	12389361
mycoplasma hominis infection in heart and lung transplantation.	"culture-negative" wound infection and mediastinitis secondary to mycoplasma hominis have been reported after cardiothoracic surgery but no case cluster has ever been described. we report 4 cases of infection in 3 cardiac and 1 bilateral sequential lung transplant recipient over 3 weeks of hospitalization. successful treatment was achieved with early aggressive surgical intervention and combination antibiotics of clindamycin, doxycycline and/or ciprofloxacin. this cluster raises the question of ...	2002	12431497
detection of bacterial vaginosis-related organisms by real-time pcr for lactobacilli, gardnerella vaginalis and mycoplasma hominis.	the aim of this study was to determine the feasibility of detecting bacterial vaginosis (bv)-related organisms in stored genital tract specimens using real-time pcr. frozen cervicovaginal lavage (cvl) samples from 21 women were analyzed by real-time pcr for the numbers of mycoplasma hominis, gardnerella vaginalis and lactobacilli. lactobacilli organisms were detected in all cvl samples, g. vaginalis was detected in all but one sample, while m. hominis was detected in only six samples. using the ...	2002	12443827
mycoplasma hominis and ureaplasma species brain abscess in a neonate.	we report an infant with a mixed mycoplasma and ureaplasma brain abscess who was successfully treated with intravenous doxycycline and erythromycin. therapeutic concentrations of doxycycline were demonstrated in cerebrospinal fluid. this report is evidence of the potential for mycoplasma and ureaplasma to produce focal central nervous system infection, as well as meningitis, in neonates and that use of doxycycline can be efficacious in the therapy of such infections.	2002	12458575
drift of tetm determinant in urogenital microbiocenosis containing mycoplasmas during treatment with a tetracycline antibiotic.	we studied the correlation between genetic transfer of tetm determinant in tn916 conjugative transposon by urogenital mycoplasmas (mycoplasma hominis and ureaplasma urealyticum) and changes in the bacterial repertoire during treatment with a tetracycline antibiotic. basic conditions favoring the nonspecific transfer of tetm determinant into mollicute cells are determined and the allele polymorphism of tetm determinant in clinical strains of m. hominis and u. urealyticum is evaluated. the structu ...	2002	12459871
[prevalence of associated microorganisms in genital discharge, argentina].	there was a significant increase in the number of women demanding gynecological care in public hospital of the great buenos aires, argentina, between 1997 and 1998. it was necessary to update the prevalence of associated microorganisms in order to review the laboratory support and adjust prevention and control guidelines.	2002	12471378
phylogeny-based rapid identification of mycoplasmas and ureaplasmas from urethritis patients.	some strains of mycoplasmas and ureaplasmas (family mycoplasmataceae) are associated with nongonococcal urethritis (ngu) or other genitourinary infections. we have developed a rapid and reliable method of identifying the presence and prevalence of mycoplasmas and ureaplasmas in men with ngu. this method is based on the amplification of a part of the 16s rrna gene by pcr and phylogenetic analysis. a portion of the 16s rrna gene from 15 prototype strains was amplified with a set of common primers, ...	2002	11773101
genital mycoplasmas, including mycoplasma genitalium, as sexually transmitted agents.	mycoplasmas are the smallest free-living organisms, widespread in nature. several mycoplasma species have been isolated from humans. for 6 of them: mycoplasma hominis, ureaplasma urealyticum, m. primatum, m. genitalium, m. spermatophilum and m. penetrans, the genital tract is the main site of colonization. this review is concentrated on the role of mycoplasmas as sexually transmitted agents, with the emphasis to m. genitalium infections. m. hominis and u. urealyticum are isolated from the genita ...	2002	11839161
induced expression of melittin, an antimicrobial peptide, inhibits infection by chlamydia trachomatis and mycoplasma hominis in a hela cell line.	as the number of pathogenic microbial strains resistant to different antibiotics increases, amphipathic peptides with antimicrobial activity are promising agents for the therapy of infectious diseases. this work deals with the effect of an amphipathic antimicrobial peptide, melittin, expressed within recombinant plasmid vectors, on infection with urogenital pathogens chlamydia trachomatis and mycoplasma hominis in hela cell culture. recombinant plasmid constructs with the melittin gene under the ...	2002	11850166
evidence of active efflux in resistance to ciprofloxacin and to ethidium bromide by mycoplasma hominis.	the uptake of fluoroquinolones was characterized for the fluoroquinolone-susceptible strain pg21 of mycoplasma hominis. accumulation of fluoroquinolones appeared to occur by passive diffusion. addition of arginine as the energizer significantly reduced the uptake of fluoroquinolones, suggesting the presence of an energy-dependent efflux process. reserpine and orthovanadate, two multidrug pump inhibitors, increased significantly the ciprofloxacin (cip) uptake. in contrast, such a strong effect wa ...	2002	11850247
meningo-encephalitis in a neonate caused by maternal mycoplasma hominis treated successfully with chloramphenicol.		2002	11863270
mycoplasma hominis infection in renal transplantation.		2002	11865099
intrauterine infection and prematurity.	intrauterine infection is a major cause of premature labor with and without intact membranes. intrauterine infection is present in approximately 25% of all preterm births and the earlier the gestational age at delivery, the higher the frequency of intra-amniotic infection. microorganisms may also gain access to the fetus before delivery. a fetal inflammatory response syndrome elicited in response to microbial products is associated with the impending onset of preterm labor and also with multi-sy ...	2002	11921380
ureaplasma urealyticum biovar determination in women attending a family planning clinic in guiné-bissau, using polymerase chain reaction of the multiple-banded antigen gene.	although ureaplasma urealyticum is commonly found in the genital tract of asymptomatic women, it has been suggested that only certain subgroups of this microorganism are disease associated. vaginal specimens were collected to determine the distribution of u. urealyticum biovars and to estimate their possible association with age, absence of lactobacilli, and tetracycline resistance. of the 94 women studied, 40 (43%) carried u. urealyticum and were biotyped by polymerase chain reaction (pcr). twe ...	2002	11948794
in vitro activities of abt-773 and other antimicrobials against human mycoplasmas.	the in vitro susceptibilities of 103 mycoplasma pneumoniae isolates, 14 mycoplasma hominis isolates, 12 mycoplasma fermentans isolates, and 24 ureaplasma species to abt-773, an investigational ketolide, and seven other agents were determined. for m. pneumoniae, the abt-773 mic at which 90% of isolates are inhibited (mic(90); <or=0.001 microg/ml) was comparable to those of azithromycin, clarithromycin, and erythromycin and at least 128-fold lower than those of levofloxacin, gatifloxacin, moxiflox ...	2003	12499166
in vitro susceptibilities to and bactericidal activities of garenoxacin (bms-284756) and other antimicrobial agents against human mycoplasmas and ureaplasmas.	the in vitro susceptibilities to garenoxacin (bms-284756), an investigational des-fluoroquinolone, and eight other agents were determined for 63 mycoplasma pneumoniae, 45 mycoplasma hominis, 15 mycoplasma fermentans, and 68 ureaplasma sp. isolates. garenoxacin was the most active quinolone, inhibiting all isolates at <or=1 microg/ml. the garenoxacin mic at which 90% of isolates are inhibited (mic(90)s; <or=0.008 microg/ml) was at least 4-fold less than those of moxifloxacin and clindamycin, 8-fo ...	2003	12499185
[mycoplasmal infection in patients with reactive arthritis and seronegative spondyloarthropaties].	after investigation of 150 patients with reactive arthritis and undifferentiated spondyloarthropathies, inflammation signs of urogenital tract (uretritis in men and endocervicitis in women) were found in about 2/3 of patients. in those patients with reactive arthritis and undifferentiated spondyloarthropathies ureaplazma urealyticum was found in more than 1/4 of patients, mycoplasma hominis - in 1/10 of patients, and both microbes appeared in 1/10 of patients. micoplasmal infection occurred in e ...	2003	12794368
[association between genital mycoplasmas and risk of preterm delivery].	the main aim of this study was to determine the prevalence of maternal colonisation by genital mycoplasmas during pregnancy and to assess its association with preterm delivery. the cervical swabs of 179 pregnant women between 8 and 16 week of gestation were cultured for mycoplasma hominis and ureaplasma urealyticum. clinical details and perinatal outcomes were documented. of a total of 179 women enrolled, 68 (38.0%) had positive cervical culture for genital mycoplasmas; 30 (16.7%) for m. hominis ...	2003	15537264
prevalence of ureaplasma urealyticum and mycoplasma genitalium in men with non-gonococcal urethritis.	this study in teheran, islamic republic of iran, investigated the prevalence of ureaplasma urealyticum and mycoplasma species in men with non-gonococcal urethritis. urethral swab samples were collected from 125 cases and 125 healthy men as a control group. the samples were then investigated by culture methods. the rates of detected bacteria in case and control groups were 19.2% and 7.2% for u. urealyticum, 7.2% and 0.8% for m. genitalium, and 2.4% and 1.6% for m. hominis respectively. statistica ...	2003	15751921
[role of bacteria associated with sexually transmitted infections in the etiology of lower urinary tract infection in primary care].	urinary tract infections (uti) are the second most frequent type of infectious pathology treated in primary care clinics. the participation of microorganisms associated with sexually transmitted infection has been reported as a cause of uti; nevertheless this concept is still controversial. to gather data on this subject, we carried out a search for gardnerella vaginalis, ureaplasma urealyticum, mycoplasma hominis and streptococcus agalactiae besides the common microorganisms involved in uti.	2003	12586032
agents of non-gonococcal urethritis in males attending an israeli clinic for sexually transmitted diseases.	non-gonococcal urethritis is the most common clinical diagnosis in men seeking care at clinics for sexually transmitted diseases.	2003	12592953
efficacy and safety of azithromycin as monotherapy or combined with metronidazole compared with two standard multidrug regimens for the treatment of acute pelvic inflammatory disease.	the objective of the study was to compare the efficacy of azithromycin, alone or with metronidazole, versus two standard multidrug regimens for the treatment of acute pelvic inflammatory disease (pid). patients with pid were treated with once-daily intravenous (i.v.) azithromycin 500 mg for 1 day or 2 days followed by once-daily azithromycin 250 mg orally for a total of 7 days, alone or with three-times-daily metronidazole 400 mg or 500 mg i.v. then orally for a total of 12-14 days. the comparat ...	2003	12635534
microbial invasion and cytokine response in amniotic fluid in a swedish population of women in preterm labor.	previous studies indicate an association between intra-amniotic microbial invasion and/or inflammation and spontaneous preterm birth, but there is a limited amount of data available from europe. the aim of this study was to investigate the occurrence of intra-amniotic microorganisms and cytokines (interleukin-6 and interleukin-8) in a swedish population of women in preterm labor and their correlation with preterm birth.	2003	12648172
antenatal mycoplasma infection, the fetal inflammatory response and cerebral white matter damage in very-low-birthweight infants.	we address the question as to whether ureaplasma urealyticum or mycoplasma hominis, cultured from the placenta of very-low-birthweight (vlbw) infants, are associated with an increased risk of (a) fetal vasculitis and (b) ultrasonographic cerebral white matter echolucency. the sample consisted of 464 vlbw infants for whom (i) the surface of the chorion was cultured for u. urealyticum and m. hominis; (ii) the placenta was examined histologically; and (iii) a cranial ultrasound scan was obtained cl ...	2003	12562472
mycoplasma disease and acute chest syndrome in sickle cell disease.	acute chest syndrome (acs) is the leading cause of hospitalization, morbidity, and mortality in patients with sickle cell disease. radiographic and clinical findings in acs resemble pneumonia; however, etiologies other than infectious pathogens have been implicated, including pulmonary fat embolism (pfe) and infarction of segments of the pulmonary vasculature. the national acute chest syndrome study group was designed to identify the etiologic agents and clinical outcomes associated with this sy ...	2003	12837872
pneumonia due to mycoplasma hominis in a healthy adult.	a case of pneumonia due to mycoplasma hominis in a healthy adult is presented. the bacterium was diagnosed by a quantitative culture method and identification to the species level by sequence analysis of 16s rrna gene. the objective of this presentation is to bring to attention the need to search for this opportunistic pathogen. mycoplasma may be an important cause of bacterial pneumonia without microbiological diagnosis and its incidence may be underestimated.	2003	12839163
do microbiological factors account for poor pregnancy outcome among unmarried pregnant women in poland?	being unmarried is a well-known risk factor for poor pregnancy outcome such as preterm delivery and intrauterine growth restriction. the aim of this prospective study was to assess the prevalence and risk of bacterial vaginosis (bv) and selected bacteria isolated from the lower genital tract and to determine the socioeconomic and microbiological characteristics that might be responsible for poor pregnancy outcome observed among unmarried pregnant women.	2003	12913347
[clinical application research of feilinjian peroral liquid in the treatment of chlamydia and mycoplasma infectious prostatitis].	to evaluate the validity and security of feilinjian peroral liquid in the treatment of chlamydia trachomatis (ct), mycoplasma hominis (mh) and ureaplasma urealyticum (uu) infectious prostatitis.	2003	12931378
seeding of a cavernous angioma with mycoplasma hominis: case report.	to describe a unique case of hematogenous seeding of a cavernous angioma with the commensal organism mycoplasma hominis.	2003	12943591
susceptibility of mixed infection of ureaplasma urealyticum and mycoplasma hominis to seven antimicrobial agents and comparison with that of ureaplasma urealyticum infection.	in order to investigate the susceptibility of mixed infection of ureaplasma urealyticum (uu) and mycoplasma hominis (mh) to 7 kinds of antimicrobial agents and comparison with that of uu infection in ngu patients, the in vitro susceptibility was determined by using microdilution method. the positive results were analyzed. the results showed that the sequence of susceptibility to 7 kinds of antimicrobial agents for both uu infection group and uu-mh mixed infection group was almost the same from t ...	2003	12973952
bacteremia with mycoplasma hominis and ureaplasma urealyticum in patients undergoing hysterectomy.	in the study presented here, the aim was to investigate the frequency and clinical significance of bacteremia with urogenital mycoplasmas in immunocompetent patients following gynecological surgery. among 56 patients undergoing elective hysterectomy (mean age, 55 years; range, 32-82 years), mycoplasma hominis and ureaplasma urealyticum were detected in urine by pcr in 2 and 11 patients, respectively. pre- and postoperative blood samples of the colonized patients were investigated for the coloniz ...	2003	13680393
genital mycoplasmas in women attending a family planning clinic in guiné-bissau and their susceptibility to antimicrobial agents.	a study on the prevalence of genital mycoplasmas and their susceptibility to the most common antimicrobial agents used for treating the infection was conducted on 94 women attending a family planning clinic in guiné-bissau. fifty-four women (57.4%) were positive for mycoplasma hominis and/or ureaplasma urealyticum. m. hominis and u. urealyticum separately isolated from infected women yielded frequencies of 31.5 and 27.8%, respectively, the remainder were infected with both species. no strain was ...	2003	12711099
assessment of antibiotic susceptibility of ureaplasma urealyticum from prostitutes and outpatient clinic patients using the e-test and agar dilution method.	in this study, a total of 647 vaginal discharge samples were examined. ureaplasma urealyticum growth was seen in 68 samples (10.5%). the antibiotic sensitivity of 30 types of u.urealyticum was determined with the e-test and agar dilution method. with the agar dilution method, all types were sensitive to ciprofloxacin and ofloxacin (mic 0.94 microg/ml), tetracycline (mic 0.125 microg/ml) and doxycycline (mic 0.125 and 0.190 microg/ml). furthermore, with the agar dilution method, 18 types (60%) we ...	2003	12714809
[cloning and expression of the mycoplasma hominis ftsz for a cell division protein].	a mycoplasma hominis chromosomal fragment containing the full-length ftsz gene was cloned and sequenced. natural expression of this gene was demonstrated by reverse transcription-polymerase chain reaction (rt-pcr) with total rna. the m. hominis ftsz protein was shown to differ substantially from its counterparts of two other mycoplasma species, m. genitalium and m. pneumoniae. the possibility of m. hominis ftsz expression in escherichia coli was demonstrated with several bacterial strains. the m ...	2003	12722630
rapid detection of mycoplasma genitalium, mycoplasma hominis, ureaplasma parvum, and ureaplasma urealyticum organisms in genitourinary samples by pcr-microtiter plate hybridization assay.	we present a method for detecting the presence of mycoplasma genitalium, mycoplasma hominis, ureaplasma parvum, and ureaplasma urealyticum organisms, which are thought to be associated with nongonococcal urethritis (ngu) and other genitourinary infections, in clinical samples. this method consists of pcr amplification of a part of the 16s rrna gene followed by 96-well microtiter plate hybridization assay using four species-specific capture probes to detect the targets. to test the efficacy of th ...	2003	12734216
detection of several mycoplasma species at various anatomical sites of homosexual men.	in order to determine the colonisation patterns of several mycoplasma species in homosexual men, urethral, oral and rectal specimens from 10 homosexual men with acute non-gonococcal urethritis (ngu) and 18 without ngu were examined using sensitive methods. mycoplasma hominis and ureaplasma urealyticum existed in both groups, which is in keeping with previous studies of heterosexual men. mycoplasma genitalium was detected in the rectum of both ngu-positive and ngu-negative men and in the urethra ...	2003	12734722
a case of mycoplasma hominis meningo-encephalitis in a full-term infant: rapid recovery after start of treatment with ciprofloxacin.	the role of mycoplasma hominisas a causative agent for neonatal sepsis and meningitis is still unclear. meningitis secondary to m. hominisis well-described in the literature; however, m. hominiscan also be isolated from cerebrospinal fluid (csf) obtained from infants without signs of meningitis. we present a case of a full-term infant with meningo-encephalitis with seizures, epileptic activity on the eeg, inflammation of brain tissue on a ct scan, and cloudy csf containing elevated cell counts, ...	2003	12740695
mycoplasma hominis lipid-associated membrane protein antigens for effective detection of m. hominis-specific antibodies in humans.	lipid-associated membrane proteins (lamps) from 14 mycoplasma hominis isolates or strains share similar protein and antigenicity profiles. of 31 human immunodeficiency virus-infected patients from whose samples m. hominis was cultured, 28 tested strongly positive for serum antibodies to m. hominis lamps. the remaining 3 serum samples showed low antibody titer to lamps from all of the 14 m. hominis isolates or strains, which was likely the result of the compromised immune systems of the patients. ...	2003	12746769
can known risk factors explain racial differences in the occurrence of bacterial vaginosis?	black women are more likely to have bacterial vaginosis (bv) than are non-hispanic white women. we examined whether this disparity can be explained by racial differences in known bv risk factors.	2003	12749680
microbial invasion and cytokine response in amniotic fluid in a swedish population of women with preterm prelabor rupture of membranes.	previous studies have shown an association between intra-amniotic microbial invasion and/or inflammation and spontaneous preterm birth. the aim of this study was to investigate the occurrence of intra-amniotic microorganisms and cytokines [interleukin (il)-6 and il-8] in a swedish population, with low incidence of preterm birth, of women with preterm prelabor rupture of membranes and their correlation to preterm birth.	2003	12752072
aetiological diagnosis of brain abscesses and spinal infections: application of broad range bacterial polymerase chain reaction analysis.	to evaluate the usefulness of the broad range bacterial rdna polymerase chain reaction (pcr) method combined with dna sequencing in the aetiological diagnosis of intracranial or spinal infections in neurosurgical patients.	2003	12754340
dna gyrase and topoisomerase iv mutations in clinical isolates of ureaplasma spp. and mycoplasma hominis resistant to fluoroquinolones.	twelve clinical isolates of ureaplasma spp. and one isolate of mycoplasma hominis were examined for resistance to fluoroquinolones. previously described mutations at positions 83 and 95 in gyra (escherichia coli numbering) and positions 80 and 87 in parc were found. unusual alterations were described at positions parc 123 and 134.	2003	14506049
mycoplasma hominis infection of a subdural haematoma in the peripartum period.		2003	14555394
[genital mycoplasmas].	the mycoplasmas frequently isolated in genital tract, and potentially pathogenic, are m. hominis, u. urealyticum and m. genitalium. m. hominis and u. urealyticum are very frequent in the vaginal tract and they can be, according to circumstances, either commensal or pathogenic. m. hominis and/or u. urealyticum, have been considered as responsible for many types of genital infectious diseases (such as cervicitis, pelvis inflammatory disease), for infertility, obstetrical pathologies (premature del ...	2003	14623561
comparative in vitro susceptibilities and bactericidal activities of investigational fluoroquinolone abt-492 and other antimicrobial agents against human mycoplasmas and ureaplasmas.	we determined in vitro susceptibilities for abt-492 and other antimicrobials against mycoplasma pneumoniae, mycoplasma fermentans, mycoplasma hominis, and ureaplasma species. abt-492 mics were < or =1 microg/ml, and the agent was bactericidal against selected isolates of m. pneumoniae and m. hominis. abt-492 has potential for treatment of infections due to these microorganisms.	2003	14638513
ureaplasma urealyticum and mycoplasma hominis in men attending for routine semen analysis. prevalence, incidence by age and clinical settings, influence on sperm characteristics, relationship with the leukocyte count and clinical value.	to investigate the clinical value of the screening of ureaplasma urealyticum and mycoplasma hominis in routine semen analysis.	2003	14646436
folate biosynthesis pseudogenes, psifolp and psifolk, and an o-sialoglycoprotein endopeptidase gene homolog in the phytoplasma genome.	phytoplasmas are wall-less phytopathogenic prokaryotes of small genome sizes that are obligate parasites of insect vectors and plant hosts. we have cloned a clover phyllody (cph) phytoplasma dna locus containing five potential coding sequences. two were identified as pseudogenes (psifolp and psifolk) homologous to folp and folk genes, which encode dihydropteroate synthase (dhps) and 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (hppk), respectively, in other bacteria. evolution of the phyt ...	2003	14659042
[prevalence and drug tolerance of mycoplasma in patients with urogenital inflammation].	to observe the prevalence and drug tolerance of mycoplasma(ureaplasma urealyticum and mycoplasma hominis) in patients with urogenital inflammation.	2003	14689895
mycoplasma endocarditis: two case reports and a review.	we describe 2 patients with endocarditis for whom blood cultures and cardiac valve cultures were repeatedly sterile. broad-range eubacterial polymerase chain reaction (pcr) amplification performed on cardiac valve specimens from these 2 patients detected dna of mycoplasma hominis, for one patient, and of ureaplasma parvum, for the other patient. three other cases of infective endocarditis caused by mycoplasmas were identified in the literature. it is important to rule out a diagnosis of mycoplas ...	2004	14727231
cc chemokine concentrations increase in respiratory distress syndrome and correlate with development of bronchopulmonary dysplasia.	inflammation is one of the primary processes underlying respiratory distress syndrome (rds) and its evolution into bronchopulmonary dysplasia (bpd). recruitment and subsequent activation of macrophages in the lung are mediated by cc chemokines. the role of cc chemokines has not been extensively studied in the course of rds. serial tracheal aspirates (ta) were obtained from 56 mechanically ventilated infants with birth weights less than 1,500 g during intervals in the first 21 days of life. trach ...	2004	14730659
oppa, the substrate-binding subunit of the oligopeptide permease, is the major ecto-atpase of mycoplasma hominis.	most atpases, involved in energy-driven processes, act in the cytoplasm. however, external membrane-bound atpases have also been described in parasites and eukaryotic cells. in mycoplasma hominis, a bacterium lacking a cell wall, the surface-exposed substrate-binding protein oppa of an oligopeptide permease (opp) contains an atp binding p-loop structure in the c-terminal region. with atp affinity chromatography and tryptic digestion in the presence or absence of atp, the functionality of the mg( ...	2004	14761996
prevalence and treatment of chlamydia trachomatis, ureaplasma urealyticum, and mycoplasma hominis in patients with non-gonococcal urethritis.	the aim of present study was to evaluate the occurrence of chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum in non-gonococcal urethritis (ngu) and to determine the bacterial resistance to six antibiotics in order to determine the most suitable treatment strategy. a total of 50 patients were enrolled into the study. urethral samples were taken with a dacron swab placed into urethra 2 - 3 cm in males, and vaginal samples were taken from the endocervical region in women. the pa ...	2004	14985631
severe mycoplasma hominis infections in two renal transplant patients.	systemic infections due to mycoplasma hominis are rare and occur mainly in immunocompromised patients. reported here are the cases of two renal transplant patients with peritonitis who did not respond to empirical antimicrobial treatment. effective treatment with doxycycline was administered only after definitive identification of mycoplasma hominis was achieved. for this identification, the new genetic amplification-sequencing method was invaluable.	2004	14986166
synthesis, anti-mycobacterial, anti-trichomonas and anti-candida in vitro activities of 2-substituted-6,7-difluoro-3-methylquinoxaline 1,4-dioxides.	a new series of 23 6,7-difluoro-3-methyl-2-phenylthio/phenylsulfonyl/phenylsulfinyl/benzylamino/phenylamino-quinoxaline 1,4-dioxides variously substituted in the phenyl moiety, was synthesized and submitted to in vitro evaluation for anti-mycobacterial, anti-trichomonas, anti-candida, anti-mycoplasma and antibacterial activities. in anti-mycobacterial assays, several compounds resulted active (mic90 = 2.0-4.0 microg/ml) against mycobacterium tuberculosis h37rv. anti-trichomonas screening showed ...	2004	14987828
simultaneous detection and identification of common cell culture contaminant and pathogenic mollicutes strains by reverse line blot hybridization.	we have developed a reverse line blot (rlb) hybridization assay to detect and identify the commonest mollicutes causing cell line contamination (mycoplasma arginini, mycoplasma fermentans, mycoplasma hyorhinis, mycoplasma orale, and acholeplasma laidlawii) and human infection (mycoplasma pneumoniae, mycoplasma hominis, mycoplasma genitalium, ureaplasma parvum, and ureaplasma urealyticum). we developed a nested pcr assay with "universal" primers targeting the mollicute 16s-23s rrna intergenic spa ...	2004	15006769
cervical immunoglobulin a and altered vaginal flora in pregnant women with threatened preterm delivery.	the aim of our study was to investigate the cervical immunoglobulin a concentration in women with threatened preterm delivery. immunoglobulin a concentration in the cervical mucus of 80 women with symptoms of preterm delivery was measured using radial immunodiffusion. the results were compared with those of 60 healthy pregnant women. concentrations of immunoglobulins in maternal serum were also measured. there was no significant difference of cervical immunoglobulin a (iga) concentration between ...	2004	15008384
susceptibility testing of singapore strains of mycoplasma hominis to tetracycline, gatifloxacin, moxifloxacin, ciprofloxacin, clindamycin, and azithromycin by the etest method.	the minimal inhibitory concentrations of tetracycline, gatifloxacin, moxifloxacin, ciprofloxacin, clindamycin and azithromycin for 28 singapore clinical mycoplasma hominis strains were studied using the etest method. different incubation conditions did not affect susceptibility categories, except for tetracycline intermediate-susceptibility strains. tetracycline-susceptibility was only 35.7%. all strains were susceptible to the fluoroquinolones and clindamycin but resistant to azithromycin.	2004	15023431
[male urogenital tract mycoplasma infection and drug-resistance evolution].	to investigate the male urogenital tract mycoplasma infection and drug-resistance evolution in 2001 and 2003.	2004	15027187
possible role of bacterial and viral infections in miscarriages.	to determine the role of infections in miscarriages. chorionic villi from aborted material were subjected to cytogenetic evaluation and analyzed for the presence of chlamydia trachomatis, ureaplasma urealyticum, mycoplasma hominis, human cytomegalovirus (hcmv), adeno-associated virus (aav), and human papillomaviruses (hpv).	2004	15037417
[relationship between the endocervical mycoplasma infection and spontaneous abortion due to early embryonic death].	to study the relationship between endocervical mycoplasma infection and the spontaneous abortion due to the early embryonic death and the drug sensitivity to mycoplasma.	2004	15059581
comparison of multiplex pcr assay with culture for detection of genital mycoplasmas.	ureaplasma, spp. mycoplasma genitalium, and mycoplasma hominis are associated with infection of the genitourinary tract, reproductive failure, and neonatal morbidity and mortality. we have developed a multiplex pcr for the detection of these mycoplasmatales in a single amplification reaction. the analytical sensitivities of this assay were 10.8, 10.8, and 8.8 cfu for each organism, respectively. this multiplex pcr was compared to culture on 26 cervical swabs, 2 vaginal swabs, 4 female urine spec ...	2004	15070999
experimental mycoplasma hominis infection of the genital tract in balb/c mice.	we studied the ability of laboratory and clinical strains of mycoplasma hominis to colonize the genital tract mucosa in balb/c mice. colonization with mycoplasma occurred only in mice receiving estrogen. mycoplasma hominis strains obtained after 3-fold passage through the vaginal mucosa in mice and administered intravaginally in a dose of 0.5 x 10(8) cfu/ml caused infection in 100% animals. inflammation in the lower genital tract was reduced by the 8th week after infection.	2004	15085246
[prevalence of asymptomatic sexually transmitted infections among high-risk patients attending a free anonymous hiv-screening center].	the aim of this study was to assess the prevalence of sexually transmitted infections among patients attending an anonymous hiv screening center.	2004	15107742
[studies on the infection status of seven species mycoplasma, three species of chlamydia, neisseria gonorrhoeae and garderella vaginalis in 76 patients with sexual transmitted diseases].	to study the infectious status of seven species of mycoplasma, three species of chlamydia, neisseria gonorrhoeae and garderella vaginalis in the 76 male sexual transmitted disease (std) patients in yangzhou city.	2004	15132870
spreading factors of mycoplasma alligatoris, a flesh-eating mycoplasma.	mycoplasma alligatoris causes lethal invasive disease of alligators and caimans. a homolog of the nagh gene, encoding a hyaluronidase secreted by clostridium perfringens, and a c. perfringens hyaluronidase nagi or nagk pseudogene were discovered in the m. alligatoris genome. the nagh gene was detected by pcr in the closest relative of m. alligatoris, mycoplasma crocodyli, but not in 40 other species representing the mycoplasma hominis, mycoplasma pneumoniae, and spiroplasma phylogenetic clusters ...	2004	15175306