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[studies on the locality and the function of spirosome in bacterial cells: relationship between the production of spirosome and anaerobic glycolysis].the locality and the function of spirosome were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and electron microscopy in peptostreptococcus productus, clostridium sp. hd-17, lactobacillus fermentum, eubacterium aerofaciens and escherichia cali b. when the bacterial cells in phosphate buffered saline were disrupted by sonication and fractionated by differential centrifugation, the spirosome protein was found in the cytoplasmic fraction and not in the fraction of ...19938315808
cleavages of the o- and c-glucosyl bonds of anthrone and 10,10'-bianthrone derivatives by human intestinal bacteria.a strictly anaerobic bacterium, bifidobacterium sp. sen, capable of hydrolyzing the o-glucosyl of sennosides was isolated from human feces. the bacterium stepwisely hydrolyzed sennoside b to sennidin b through sennidin-8-monoglucoside in pyf medium but not in gam broth. addition of d-glucose to pyf medium resulted in loss of the hydrolyzing activity in culture but addition of d-fructose did not affect the activity. coculture of this bacterium with peptostreptococcus intermedius led to rapid accu ...19938234419
microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis.a series of 993 subgingival microbial samples sent to a diagnostic microbiology laboratory included 196 samples that could be identified as compatible with a clinical diagnosis of refractory or recurrent periodontitis. in descending order of prevalence the associated microbiota included bacteroides forsythus (84%), spirochetes (83%), motile rods (76%), fusobacterium species (68%), porphyromonas gingivalis (63%), campylobacter rectus (47%), capnocytophaga species (38%), prevotella intermedia (23% ...19938463936
infected epidermoid cyst of the sphenoid bone.an infected epidermoid cyst presented on ct as a primarily dense, sclerotic expansile lesion in the greater wing of the sphenoid bone. presumably, infection was responsible for the atypical appearance.19938517372
recent taxonomic changes for anaerobic gram-positive and selected gram-negative organisms.the strains formerly classified as streptococcus anginosus or "streptococcus milleri" have now been recognized as three distinct species, streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. streptococcus morbillorum has been transferred into the genus gemella. four new species within the genus peptostreptococcus were recently named. a recent addition to the genus clostridium is clostridium argentinense, which includes clostridium botulinum type g. two new species ...19938324113
anaerobic bacterial infections of the lung and pleural space.in 193 reviewed cases of pleuropulmonary infections involving anaerobic bacteria, the predominant clinical syndromes were aspiration pneumonia, lung abscess, and empyema. most patients had an indolent infection with tissue necrosis (abscess or bronchopulmonary fistula) at initial presentation. nevertheless, many had a more fulminant disease course resembling that of pneumococcal pneumonia. transtracheal aspiration was the procedure most commonly used to obtain uncontaminated specimens for anaero ...19938324127
[anaerobic respiratory infection--evaluation of methods of obtaining specimens].anaerobic cultures were performed on various kinds of specimens obtained from 67 patients with respiratory infections. the infections consisted of 33 cases of pneumonia, 14 cases of lung abscess, 6 cases of pyothorax, 9 cases of chronic lower respiratory tract infection, and 5 cases of infection of pulmonary cyst. specimens included bronchoscopic specimens obtained using a single tube or protected catheter brush (pcb), transtracheal aspirate, percutaneous lung aspirate, aspirate using a suction ...19948207291
a quantitative comparison of selected bacteria in human carious dentine by microscopic counts.the levels of selected cariogenic and obligately anaerobic bacteria have been compared in carious dentine taken from fissures, smooth surfaces, and root surfaces. the numbers of infected dentinal tubules were determined by immunohistological staining using species-specific antisera against selected bacteria. selective localization was observed; mutans streptococci were the predominant bacteria in dentine from fissures and smooth surface coronal caries, but not from root surface caries. the propo ...19948033185
relationship between salivary blood group antigens, microbial flora and periodontal condition in young adults.to investigate a possible rôle for salivary blood-group antigens in the relative frequencies of selected periodontal pathogens and commensal oral micro-organisms, we studied the clinical and microbiological condition in young adults with or without blood group reactive substances in saliva (secretors or non-secretors respectively). clinical measurements were recorded at the ramfjord teeth in 81 1st-year dental students. in addition, presence of interproximal loss of attachment (la) was assessed ...19948157769
iron-sulfur cluster-containing l-serine dehydratase from peptostreptococcus asaccharolyticus: correlation of the cluster type with enzymatic activity.investigations were performed with regard to the function of the iron-sulfur cluster of l-serine dehydratase from peptostreptococcus asaccharolyticus, an enzyme which is novel in the class of deaminating hydro-lyases in that it lacks pyridoxal-5'-phosphate. anaerobically purified l-serine dehydratase from p. asaccharolyticus revealed epr spectra characteristic of a [3fe-4s]+ cluster constituting 1% of the total enzyme concentration. upon incubation of the enzyme under air the intensity of the [3 ...19948082806
an unclassified eubacterium taxon in acute dento-alveolar abscess.the microflora of pus samples aspirated from 50 acute dento-alveolar abscesses was examined. a total of 143 bacterial strains was isolated, consisting predominantly of prevotella spp., alpha-haemolytic streptococcus spp., peptostreptococcus spp. and eubacterium spp. an unclassified asaccharolytic eubacterium taxon was encountered in 17 (34%) of the abscesses. this taxon was found to have a positive association with fusobacterium spp. and a negative association with alpha-haemolytic streptococcus ...19948107059
electrogenic glutamine uptake by peptostreptococcus anaerobius and generation of a transmembrane potential.peptostreptococcus anaerobius converted glutamine stoichiometrically to ammonia and pyroglutamic acid, and the eadie-hofstee plot of glutamine transport was biphasic. high-affinity, sodium-dependent glutamine transport (affinity constant [kt] of 1.5 microm) could be driven by the chemical gradient of sodium, and more than 20 mm sodium was required for half-maximal velocity. high-affinity glutamine transport was not stimulated or inhibited by a membrane potential (delta psi). low-affinity glutami ...19948113169
bacterial vaginosis and vaginal microorganisms in idiopathic premature labor and association with pregnancy outcome.the vaginal microflora of 49 women in idiopathic preterm labor was compared with that of 38 term controls to determine whether the presence of bacterial vaginosis (bv) and/or specific microorganisms would influence the rate of preterm delivery. demographic factors, pregnancy outcome, and reproductive history were also studied. bv, as defined by the presence of clue cells in a vaginal wet mount and characteristic microbial findings in a stained vaginal smear and vaginal culture, was more common i ...19948126176
protein pab, a mosaic albumin-binding bacterial protein representing the first contemporary example of module shuffling.some strains of the anaerobic human commensal and pathogen peptostreptococcus magnus bind human serum albumin (hsa), whereas other strains of this species express protein l, an immunoglobulin light chain-binding surface protein. a novel hsa-binding protein called protein pab was purified in one step from the culture supernatant of an hsa-binding strain of p. magnus by affinity chromatography on hsa-sepharose. the apparent size of the molecular was 47 kda on sds-polyacrylamide gel electrophoresis ...19948163519
[a case of niddm with non-clostridial gas-producing infection in the lower limb--the effects of hyperbaric oxygen therapy].a 36-year-old male was admitted because of swelling and pain in the lower left limb. he had been diagnosed as having non-insulin dependent diabetes mellitus 4 years previously and had been treated unsuccessfully with insulin. since gas formation was radiographically demonstrated in the soft tissue of the left foot, we treated him with antibiotics and hyperbaric oxygen under the diagnosis of gas gangrene. despite this therapy, the gangrenous lesions progressed. amputation of the left foot was nec ...19948151155
changes in vaginal flora during pregnancy and association with preterm birth.a prospective study of the vaginal flora changes between midtrimester and labor was undertaken in 560 women; 337 had intact membranes and were in labor when swabs were taken. of these, 45 delivered preterm. gardnerella vaginalis, ureaplasma urealyticum (both associated with preterm birth when present in midtrimester), and mycoplasma hominis commonly persisted between midtrimester and labor, particularly in the preterm cohort (24 [65%] of 37 midtrimester isolates), and these organisms were seldom ...19948077737
[antimicrobial activities of cefuroxime against recent clinical isolates].antimicrobial activity of cefuroxime axetil (cxm-ax) was compared with those of other cephem antibiotics against clinically isolated strains obtained mainly from outpatients of our center in a period from january to september of 1990 and 1993. minimum inhibitory concentrations were determined and the following results were obtained. 1. the results suggested that, compared with reports of studies conducted with clinical isolates in early 1980's, mic80 of cxm were equal to or lower against staphyl ...19948201767
prophylactic antibiotic treatment in therapeutic or complicated diagnostic ercp: results of a randomized controlled clinical study.the present study evaluated the effects of prophylactic administration of cefotaxime in patients undergoing therapeutic or complicated diagnostic ercp. one hundred patients were randomized to two groups of 50 patients each. patients in group 1 received an intravenous infusion of 2 g cefotaxime 15 minutes before endoscopy; patients in group 2 did not receive an intravenous antibiotic before ercp (control group). blood samples were drawn for bacteriologic cultures before endoscopy and 5, 15, 30, a ...19947988813
bacteria invading periapical cementum.the aim of this study was to determine whether microorganisms invade periapical cementum of human teeth from the adjacent periapical lesions. we therefore attempted to isolate microorganisms from periapical cementum through the adoption of standard anaerobic procedures for obligate anaerobes. samples of cementum were taken from 10 amputated tooth roots at the time of apicoectomy. from two of these samples, bacteria were recovered after anaerobic incubation, but no bacteria were recovered after a ...19948035155
determination of 16s ribosomal rna sequences of all members of the genus peptostreptococcus and their phylogenetic position.a comparative analysis of 16s ribosomal rna sequences of all peptostreptococcus species revealed that most members of the genus peptostreptococcus should be divided into many different genera. the relationship between clostridia and peptostreptococci was analysed to find the phylogenetic position of peptostreptococci.19948132148
case report: anaerobic meningitis caused by peptostreptococcus magnus after head and neck surgery.although anaerobic bacterial meningitis is uncommon, patients subjected to resection of head and neck malignancy appear at special risk. in this article, the authors report on a 72-year-old man in whom meningitis developed after extensive resection of the right sinuses for squamous cell carcinoma; initial treatment consisted of intravenous vancomycin and ceftazidime. intravenous penicillin g was added after the fortuitous early finding of intracellular cocci in wright-giemsa stained cerebral spi ...19948074138
the clinical importance of gram-positive anaerobic cocci isolated at st bartholomew's hospital, london, in 1987.the clinical importance of the gram-positive anaerobic cocci (gpac) isolated in 1987 at st bartholomew's hospital, london, is assessed. of about 800 anaerobic isolates, 209 (27%) were gpac, of which 67 (32%) were from abscesses and 22 (11%) were in pure growth. four species comprised 77% of the 168 isolates available for study: peptostreptococcus magnus (55 isolates, 33%), p. micros (23, 14%), p. asaccharolyticus (24, 14%) p. asaccharolyticus (24, 14%) and p. anaerobius (27, 16%). different spec ...19948006943
[brain abscess complicating dental caries in children].brain abscess following dental or periapical infection is rare in childhood. this report describes brain abscesses found in two children with dental caries. case 1.--a 12 year-old boy was admitted because he had suffered from acute meningitis for 3 days. clinical examination showed symptoms of meningitis plus palsy of the right third and fourth cranial nerves and of the left facial nerve, and a defect in the left temporal field. funduscopic examination showed papilledema; ct scan and mri showed ...19947987445
[an evaluation of pathogens in patients with bronchopulmonary infection by transtracheal aspiration: december 1978-march 1993].we performed transtracheal aspiration (tta) in 1165 patients, who were suspected to have bronchopulmonary infection, from december 1978 to march 1993. we isolated pathogens from tta in 806 patients (69.2%). we isolated h. influenzae (62 cases), s. pneumoniae (39 cases) and m. catarrhalis (24 cases) in patients with acute bronchitis, s. pneumoniae (65 cases), alpha-streptococcus sp. (52 cases), h. influenzae (32 cases) and s. aureus (29 cases) in patients with pneumonia or lung abscess and h. inf ...19947996025
identification of five peptostreptococcus species isolated predominantly from the female genital tract by using the rapid id32a system.the rapid id32a kit (biomérieux vitek, inc., hazelwood, mo.) was evaluated for its ability to identify peptostreptococcus species compared with conventional biochemical tests and gas-liquid chromatography (virginia polytechnic institute), the current "gold standard" method. a total of 5 peptostreptococcus american type culture collection strains and 95 clinical isolates comprising peptostreptococcus anaerobius, p. asaccharolyticus, p. magnus, p. micros, and p. prevotii isolates were included for ...19948051260
peptostreptococcal infection in children.680 peptostreptococcus sp. (ps) were recovered from 598 (34%) of 1,750 specimens obtained from 554 patients. they included 103 ps asaccharolyticus, 74 ps magnus, 56 ps prevotii, 51 ps micros, 46 ps anaerobius, 11 ps morbilorum, and 10 ps saccharolyticus. most infections were polymicrobial (in 553 instances or 92%) but in 45 (8%), ps were recovered in pure culture. most ps were isolated from abscesses (237), ears (104), peritoneal fluid (95), lung infections (66), bone (30) and sinuses (24). pred ...19947855547
the gingival immune response to periodontal pathogens in juvenile periodontitis.a gingival explant culture system was utilized to evaluate the reactivity of local immunoglobulins produced by juvenile periodontitis tissue. gingival explant culture supernatant fluids were screened, via a standardized dot-immunobinding assay, for antibodies reactive to: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, eikenella corrodens, peptostreptococcus micros, peptostreptococcus anaerobius, capnocytophaga ochracea, eubacterium no ...19947870467
delta 22-beta-muricholic acid in monoassociated rats and conventional rats.bile acids were analyzed in the bile, small and large intestines, and feces of germ-free rats after a single inoculation with one of six intestinal bacteria that had been originally isolated from human feces. bacteroides vulgatus and bifidobacterium longum preferentially deconjugated tauro-beta-muricholic acid and taurocholic acid, respectively. clostridium ramosum, peptostreptococcus productus and lactobacillus gasseri deconjugated both bile acids, but escherichia coli did not deconjugate eithe ...19948177022
antibacterial activity of clavulanate and tazobactam on peptostreptococcus spp. 19948182024
major methodology-dependent discordant susceptibility results for bacteroides fragilis group isolates but not other anaerobes.two standardized susceptibility test methods, a broth microdilution (bmd) and agar dilution (ad) method were performed on a total of 441 clinical isolates of anaerobes with ceftizoxime, cefotaxime, ceftriaxone, cefoxitin, piperacillin, and metronidazole. against the 339 strains of the bacteroides fragilis group bmd minimum inhibitory concentration (mic) values were lower than those from ad testing for all the beta-lactams. overall for the b. fragilis group and the beta-lactams, the mode mic valu ...19947874880
impaired bactericidal activity of pmn from two brothers with necrotizing ulcerative gingivo-periodontitis.the purpose of this study was to investigate the pathogenesis of necrotizing ulcerative gingivo-periodontitis (anup) diagnosed in two brothers, age 9 (anup1) and 14 (anup2) from rural egypt. complete blood count, differential and blood chemistry were within normal limits for both brothers and they were not malnourished. the phagocytosis and killing function of their polymorphonuclear leukocytes (pmn) towards four bacterial species were assessed using a fluorochrome microassay. the selection of b ...19948195981
bacterial endocarditis in patients with chronic liver disease.although patients with cirrhosis have an increased susceptibility for bacterial infections, endocarditis complicating cirrhosis has been reported only infrequently. in this study, our objective was to determine whether, bacterial endocarditis is, in fact, a complicating factor in cirrhosis.19948198106
three-dimensional solution structure of an immunoglobulin light chain-binding domain of protein l. comparison with the igg-binding domains of protein g.protein l is a multidomain protein expressed at the surface of some strains of the anaerobic bacterial species peptostreptococcus magnus. it has affinity for immunoglobulin (ig) through interaction with framework structures in the variable ig light chain domain. the ig-binding activity is located to five homologous repeats called b1-b5 in the n-terminal part of the protein. we have determined the three-dimensional solution structure of the 76 amino acid residue long b1 domain using nmr spectrosc ...19947947810
in vitro antibacterial activity of fk037, a new parenteral broad-spectrum cephalosporin, against recent clinical isolates in the fields of obstetrics and gynecology.the antibacterial activity of a new parenteral cephalosporin, fk037 was assessed against recent aerobic and anaerobic strains isolated from patients in the fields of obstetrics and gynecology during the period between january 1992 and june 1993. the mics of fk037 for 90% of the clinical isolates tested were 0.10 microgram/ml for escherichia coli and klebsiella pneumoniae, 0.20 microgram/ml for streptococcus agalactiae, 0.39 microgram/ml for gardnerella vaginalis, 0.78 microgram/ml for staphyloco ...19948205934
nucleotide sequence of the gene for peptostreptococcal protein l.a gene bank of peptostreptococcus magnus dna was established using an e. coli host-vector system. western blot analysis identified a clone expressing protein l which bound to the light chain of human immunoglobulins. dna sequence determination and analysis revealed an open reading frame of 992 amino acids, giving a theoretical secreted protein of 106 kd with a pl of 4.67.19947987012
distribution of clindamycin in cortical bone during direct local infusion of the canine tibia.the distribution of clindamycin in tibial cortical bone, administered via direct local infusion with an implantable pump, is described. clindamycin concentrations in cortical bone were measured after 3, 7, and 21 days of intraosseous infusion. the tibia were divided into four quadrants relative to the outflow infusion catheter site located in the medial aspect of the mid-diaphysis. a gradient of 5-30 mm from the infusion site was documented in all four quadrants (proximal lateral, proximal media ...19947996320
the bacteriology of obstructive pneumonitis. a prospective study using ultrasound-guided transthoracic needle aspiration.obstructive pneumonitis, the opacity that develops distal to an obstructing endobronchial lesion or external compression, is actually a combination of atelectasis, bronchiectasis with mucus plugging, and true parenchymal inflammation. in the majority of cases, it is usually not possible to determine whether infection is present or not from the radiographic findings alone. the aim of this study was to evaluate the bacteriology of obstructive pneumonitis and the influence of this result on the tre ...19948004324
associations of specific bacteria with some endodontic signs and symptoms.although a wide range of bacterial species has been isolated from infected dental root canals it remains necessary to determine whether any particular group of such bacteria is associated with specific endodontic symptoms and clinical signs. in this study 30 root canals were examined microbiologically; of these, 14 were associated with pain, 20 with tenderness to percussion, 23 presented with wet root canals, seven with swelling, five with purulent exudate and four with a sinus. clinical and mic ...19947751061
effect of different combinations of antibiotics on experimental septic peritonitis in rabbits.this study was undertaken to evaluate the efficacy of different combinations of antimicrobial agents in the treatment of experimental septic peritonitis. two hundred rabbits, divided into ten groups of 20 rabbits each, were used. septic peritonitis was provoked in two stages: treatment of animals by mechanical lavage or antibiotics was performed during the first stage; blood cultures, isolation and identification of aerobic and anaerobic bacteria in the peritoneal cavity were performed during th ...19947819597
[treatment of puerperal endometritis. evaluation of the efficacy and safety of clindamycin + gentamycin vs. penicillin + chloramphenicol + gentamycin].this was a prospective, single-blind, comparative study in patients with diagnosis of puerperal endometritis, carried out at the loayza hospital in lima, peru. the objective of this study was to evaluate the efficacy and safety of clindamycin and gentamicin in the management of endometritis vs. penicillin, chloramphenicol and gentamicin for 10 days. sixty-five patients were enrolled and 62 were evaluable for efficacy. both treatment groups were comparable in the pre-treatment period in terms of ...19947821833
on the interaction between single chain fv antibodies and bacterial immunoglobulin-binding proteins.using four bacterial immunoglobulin-binding proteins, we have analyzed the binding characteristics of a panel of 34 human single chain fv antibodies, expressed in e. coli and with known specificity and sequence. several of the single chain fv antibodies showed affinity for staphylococcal protein a and peptostreptococcal protein l, but not for the streptococcal proteins g or h. the affinity of the binding was higher for protein l (4.5 and 1.4 x 10(9) m-1) than for protein a (7.7 and 6.7 x 10(8) m ...19947822821
[susceptibility of strict anaerobic bacteria to antibiotics in france: a multicenter study].during 1992, the in vitro antibiotic susceptibility of 462 anaerobic bacteria was performed in 7 hospitals, by the reference agar dilution method. among the 222 bacteroides fragilis group strains, only one bacteroides fragilis strain was resistant to imipenem and all bêtalactams, even combined with bêtalactamase-inhibitors while metronidazole resistance could not be detected. one major outer membrane protein (probably a porin) was lacking in some of the six amoxicillin-clavulanic acid resistant ...19947824321
16s ribosomal dna sequences of anaerobic cocci and proposal of ruminococcus hansenii comb. nov. and ruminococcus productus comb. nov.the 16s ribosomal dna sequences of representative members of the family peptococcaceae were determined. the members of the family examined were divided into the following four phylogenetic groups: peptococcus niger atcc 27731t (t = type strain), the sarcina-peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. peptococcus niger, the type species of the family, was not related to other members of the family. peptostreptococcus anaerobius atcc 2 ...19948123556
bacterial colonization and degradation of demineralized dentin matrix in situ.lesion formation in dentin involves demineralization followed by degradation of the exposed organic matrix. proteinases from microorganisms present in the dentin are believed to play an important role in the breakdown of the dentinal collagen. in this study, the microflora colonizing decalcified dentin matrix, placed in situ, was identified. the gelatinolytic activity of the isolated strains was assessed and related to the degradation of the dentin matrix. the predominant species found were stre ...19948124693
bacteriological study of bronchoalveolar lavage in immunocompromised hosts.sixty bronchoalveolar lavage samples collected from lung cancer patients attending the pulmonary tuberculosis and chest diseases unit of the kasturba medical college hospital, manipal; were cultured for both the aerobic and anaerobic organisms. fifty nine samples yielded bacteria in pure culture. pseudomonas aeruginosa (34.8%) was the commonest aerobe, peptostreptococcus was the commonest anaerobe (45.2%) isolated. bacteroides fragilis was isolated in (23.8%) of cases. gentamycin was found to be ...19947868174
the functional units of a peptostreptococcal protein l.protein l is a cell-surface protein from peptostreptococcus which interacts with immunoglobulin kappa light chains. a gene from peptostreptococcus strain 3316 coding for protein l and fragments thereof were expressed in escherichia coli. the peptides were examined for binding to immunoglobulin and serum albumin. the four c units were shown to be responsible for binding to immunoglobulin and the four d units for binding to albumin. this protein l molecule therefore binds to albumin at a site sepa ...19947934898
changes in root canal microbiota during the development of rat periapical lesions.periapical lesions are reproducibly induced in rats by pulp exposure and infection from the oral cavity. lesions expand rapidly between day 7 and day 15-20 (active phase), with slowed expansion thereafter. in the present study we characterized the root canal microbiota present during the active phase of lesion development in this system. the mandibular first molars of sprague-dawley rats were exposed on day 0. the teeth were extracted after 7 days (n = 10 animals) and 15 days (n = 10), and the m ...19947936717
diagnosis and management of abscesses in the basal ganglia and thalamus: a survey.a review is made of the current management strategies of abscesses in basal ganglia and thalamus, based on a review of the literature and three of our own cases. clinical picture, aetiology, diagnostic, surgical treatment and outcome are discussed. stereotactic abscess puncture in combination with temporary drainage and rinsing of the abscess cavity in combination with systemic medication of antibiotics has become the management of choice with satisfactory results.19947942190
study on treatment of bacterial vaginosis with oral administration of metronidazole or cefdinir.bacterial vaginosis (bv) is considered to be one of the most common vaginal infections in women. fifteen symptomatic women with bv were enrolled in this study. ten patients with the diagnosis of bv were treated with 10 days oral administration of metronidazole (mtn), 500 mg twice a day, and five patients with cefdinir (cfdn), 300 mg three times a day. in the mtn therapy, the rate of abnormal vaginal discharge subjectively decreased from 100 to 60%, the rate of abnormal vaginal discharges objecti ...19947956461
[antimicrobial activities of cefteram against recently clinically detected and isolated strains from patients with dental infections].to investigate the antibiotic activity of cefteram (cftm), the minimum inhibitory concentrations (mics) of cftm and of the control drugs were determined against clinically isolated strains received from november 1991 to april 1993 from 19 dental facilities throughout the country, as well as against clinically isolated strains from samples obtained at this center from patients with dental infectious diseases, and the following results were obtained. 1. 430 strains were detected in 198 cases but i ...19948151907
in vitro comparison of the effectiveness of three surface disinfectants.choosing a general purpose surface disinfectant for the dental office environment is difficult because of the wide range of products available, varying claims by manufacturers and contradictory reports in the literature regarding product efficacy. this study tested the antimicrobial effectiveness of diluted-for-use o-phenyl-phenol (omni), didecyl dimethyl ammonium (basic g) and isopropanol (virahol). products were tested for antimicrobial activity at 0, 12 and 24 hours, 3, 6 and 8 days. o-phenyl ...19947523623
study of gene transfer in vitro and in the digestive tract of gnotobiotic mice from lactococcus lactis strains to various strains belonging to human intestinal flora.the use of genetically modified organisms (gmo) in dairy products requires evaluation of the dna transfer capacity from such organisms among the human intestinal microflora. thus, both in vitro and in vivo [in the digestive tract (dt) of mice] transfer from lactococcus lactis donor strains of the conjugative plasmid pil205 (cmr) and the non-conjugative plasmid pil253 (emr) to: (1) recipient strains isolated from human faecal flora bacteroides sp., bifidobacterium sp., peptostreptococcus sp. (str ...19947921350
anaerobic susceptibility testing. slight differences in inoculum size can make a difference in minimum inhibitory concentrations.in the present study, we compared the actual inoculum density from inoculated broth microdilution wells to the targeted inoculum size (10(5) cfu/well) when the inoculum was prepared using a mcfarland nephelometer to achieve the standard density. three target inoculum sizes (10(5), 5 x 10(5), and 10(6) cfu/well) were used to compare the effect of slight inoculum size increases of both atcc and clinical strains of anaerobes on mics of various antimicrobials. actual colony counts of bacteroides fra ...19947924212
peritonitis following endoscopic polypectomy in a peritoneal dialysis patient: the need for antibiotic prophylaxis. 19947927824
evaluation of minocycline and cefuzonam for antimicrobial activity against clinical isolates.the antibacterial activity of minocycline (mino) and that of cefuzonam (czon) were assessed with clinical isolates of 19 species, and compared with that of other antibiotics. mino was highly active against methicilli-sensitive staphylococcus aureus (mssa), neisseria gonorrhoeae, moraxella (branhamella) catarrhalis, haemophilus influenzae, helicobacter pylori, flavobacterium meningosepticum, acinetobacter calcoaceticus, peptostreptococcus spp. and propionibacterium acnes, but not as effective aga ...19947933531
rapid diagnosis of anaerobic gram-positive cocci by salt tolerance.the maximum salt tolerance was 2.5% in cases of peptostreptococcus anaerobius and peptococcus prevotii, 3% in ruminococcus albus, 4% in acidaminococcus fermentans, peptococcus niger and megasphaera elsdenii and 5% in peptococcus magnus. the biochemical tests being largely inactive and time consuming the findings of salt tolerance if combined with those of morphological data provide clue to the rapid identification of gram-positive anaerobic cocci.19947963612
[etiologic factors in acute inflammatory states of the abdominal cavity].three hundred and two samples of biological material from patients operated because of acute inflammatory states of abdominal cavity were investigated. both aerobic and anaerobic bacteria were investigated. among aerobic flora most frequently occurred enterococci, e. coli and pseudomonas. bacteroides and peptostreptococcus were most frequently occurring anaerobes. mixed flora was seen very often. isolated microorganisms were tested for susceptibility to ciprofloxacin, cefoperazone, cefotaxime, g ...19947967931
increased activity of a new chlorofluoroquinolone, bay y 3118, compared with activities of ciprofloxacin, sparfloxacin, and other antimicrobial agents against anaerobic bacteria.a total of 435 clinical isolates of anaerobes were tested with a broth microdilution method to determine the activity of bay y 3118 compared with those of other agents against anaerobic bacteria. all strains of bacteroides capillosus, prevotella spp., porphyromonas spp., fusobacterium spp., clostridium spp., eubacterium spp., peptostreptococcus spp., and veillonella parvula were susceptible (mics of < or = 2 micrograms/ml) to bay y 3118. against the 315 strains of the bacteroides fragilis group, ...19947979307
positive and negative associations between bacterial species in dental root canals.significant associations have been previously reported between certain pairs of bacterial species isolated from human dental root canals. the aim of this study was to examine microbiologically a more extensive series of cases, with particular reference to obligate anaerobes which accounted for 64% of total isolations. a total of 65 different species was isolated and individual root canals yielded a maximum of eleven bacterial species. highly significant positive associations (p < 0.001) were fou ...19947700162
recombinant proteins l and lg. two new tools for purification of murine antibody fragments.several bacterial cell wall proteins, like proteins a and g, with valuable affinity for immunoglobulins have been discovered and are currently employed in immunological techniques. in 1988, protein l, a bacterial cell wall protein with ig-binding capacity, was isolated from the anaerobic bacterial species peptostreptococcus magnus. binding data with immunoglobulin fragments suggested that protein l could selectively bind the variable region of human kappa light chains. more recently a recombinan ...19947736533
evaluation of the antimicrobial effectiveness of citric acid and sodium hypochlorite on the anaerobic flora of the infected root canal.this study was designed to evaluate the antimicrobial effectiveness of 25% citric acid, compared with 2.5% sodium hypochlorite, after time intervals of 5, 15, 30 and 60 min. anaerobic bacterial species were isolated from infected root canals and categorized according to gram stain as gram-positive cocci, gram-negative cocci, gram-positive rods and gram-negative rods. the experimental method used was similar to that proposed by the german society for hygiene and microbiology (dghm) with slight mo ...19947995646
evaluation of the etest for antimicrobial spectrum and potency determinations of anaerobes associated with bacterial vaginosis and peritonitis.one hundred ninety-seven anaerobic organisms (24 gardnerella vaginalis, 16 mobiluncus spp., 19 peptostreptococcus spp., 20 lactobacillus spp., 20 prevotella bivia/disiens, 81 bacteroides fragilis group, 12 clostridium spp., and five fusobacterium spp.) were processed by the etest (ab biodisk, solna, sweden) and a reference (brucella blood agar) method against 10 antimicrobial agents. for the bacterial vaginosis-associated pathogens, the etest was more reproducible and correlated acceptably with ...19947705035
the antimicrobial activity of essential oils and essential oil components towards oral bacteria.a method for reproducibly determining minimal inhibitory concentrations and minimal bactericidal concentrations of plant extracts towards fastidiously and facultatively anaerobic oral bacteria, predicated upon measurements of optical densities in microtitre plate wells, was devised. the antimicrobial properties of some botanical oils were surveyed; of these, australian tea tree oil, peppermint oil, and sage oil proved to be the most potent essential oils, whereas thymol and eugenol were potent e ...19947478759
demonstration of human immunoglobulin g fc-binding activity in oral bacteria.nonimmune binding of immunoglobulins via the fc fragment may reduce opsonization and phagocytosis of bacteria and is thus considered a virulence factor. the aim of this study was to investigate a wide range of oral bacterial strains for the presence of human immunoglobulin g (igg) fc-binding activity. a total of 132 strains representing 40 different gram-positive and gram negative bacterial species were tested for igg fc-binding activity by using a fast and simple dot blot procedure with horsera ...19947496956
in vitro activity of du-6859a against anaerobic bacteria.the activity of a new quinolone agent, du-6859a, against 330 strains of anaerobic bacteria was determined by using the national committee for clinical laboratory standards-approved wadsworth brucella laked blood agar method; the activity of du-6859a was compared with those of amoxicillin-clavulanate (2:1), chloramphenicol, ciprofloxacin, clindamycin, fleroxacin, imipenem, lomefloxacin, metronidazole, sparfloxacin, and temafloxacin. du-6859a and chloramphenicol inhibited all of the isolates at co ...19947840599
increased in vitro activity of ceftriaxone by addition of tazobactam against clinical isolates of anaerobes.a total of 461 clinical strains of anaerobes were tested using a broth microdilution test to determine the activity of the combination of ceftriaxone and tazobactam and other antimicrobials against these isolates. ceftriaxone was combined with tazobactam in ratios of 1:1, 2:1, 4:1, and 8:1 and twofold dilutions of ceftriaxone in constant concentrations to tazobactam of 2, 4, 8, 16, and 32 micrograms/ml. against beta-lactamase-producing strains of the bacteroides fragilis group, b. capillosus, an ...19947851086
microbiota of rapidly progressive periodontitis lesions in association with clinical parameters.the microbial population in 73 rapidly progressive periodontitis (rpp) lesions in 10 young adults aged 25 to 35 years (5 males, 5 females) was studied in relation to the clinical parameters probing depth, bleeding on probing, and suppuration, which were recorded at the sampled sites. porphyromonas gingivalis was found to predominate (26.7%) in 73 periodontal lesions with clinical probing depth > 6 mm, followed by bacteroides forsythus (23.6%), and prevotella intermedia (15.7%). the prevalence of ...19947853132
penicillin g and penicillin g-tinidazole treatment of experimentally induced summer mastitis--effect on elimination rates of bacteria and outcome of the disease.two udder hind quarters of ten pregnant heifers were inoculated experimentally with a combination of actinomyces pyogenes, peptostreptococcus indolicus and fusobacterium necrophorum at a dose of 9 x 10(8) colony forming units (cfu) of each species of bacteria. the development of aerobic-anaerobic mastitis was followed-up clinically and through analysis of udder secretion samples. all heifers developed acute clinical mastitis. thick, purulent secretions with foul odour were already present 24 h a ...19949133056
piperacillin and tazobactam versus clindamycin and gentamicin in the treatment of hospitalized women with pelvic infection. the piperacillin/tazobactam study group.to compare the efficacy and safety of a combination of piperacillin and tazobactam with that of clindamycin and gentamicin in the treatment of hospitalized women with infections of the upper genital tract.19948290195
[right-sided infective endocarditis with ventricular septal defect].two patients underwent surgical treatment for right-sided infective endocarditis with ventricular septal defect. in both cases, blood cultures showed peptostreptococcus, and the operation was performed at non-active phase after antibiotics therapy. the case 1 was a 7-year-old girl who was observed a vegetation on the chorda of the anterior paillary muscle by echocardiography. the defect was directly closed and the vegetation was excised. the case 2 was 22-year-old female who had been diagnosed o ...19949423075
the in vitro activity of trospectomycin against anaerobic bacteria.the authors evaluated the activity of trospectomycin, a new aminocyclitol which is characterized by good antibacterial and broad spectrum activity, in comparison with clindamycin and ampicillin on a sample of recent isolates: bacteroides fragilis (15 strains), bacteroides urealyticus (5 strains), bacteroides vulgatus (5 strains), bacteroides spp. (15 strains), prevotella melaninogenica (6 strains), porphyromonas asaccharolytica (7 strains), mobiluncus spp. (3 strains), peptococcus niger (3 strai ...19958667035
antimicrobial activity of sch 27899, oligosaccharide member of the everninomycin class with a wide gram-positive spectrum.the in vitro antimicrobial activity of sch 27899 (everninomycin), a novel oligosaccharide compound of the everninomycin class, was compared with vancomycin, chloramphenicol, clinafloxacin, teicoplanin and doxycycline against 428 clinical strains of bacteria. everninomycin base exhibited the greatest antimicrobial activity compared to other formulations against all strains tested (mic90: 0.25 microg/ml) followed by clinafloxacin and teicoplanin (mic90: 0.5 microg/ml), vancomycin (mic90: 2 microg/ ...199511866719
nonpuerperal breast infection.we undertook a microbiological study of purulent specimens from women with symptomatic breast abscesses.199518476022
the aerobic and anaerobic microbiology of pustular acne lesions.specimens from 32 pustular acne lesions that were inoculated on media supportive for the growth of aerobic and anaerobic bacteria showed bacterial growth. only aerobic or facultative bacteria were recovered in 15 (47%) specimens, only anaerobic bacteria in 11 (34%) specimens, and mixed aerobic and anaerobic bacteria in 6 (18%) specimens. a total of 57 isolates, 31 anaerobes (1.0 per specimen) and 26 aerobes (0.8 per specimen) were recovered. the predominant isolates were staphylococcus sp. (19 i ...199516887540
endodontic pathogens: propagation of infection through patent dentinal tubules in traumatized monkey teeth.periapical pathology indicating endodontic infection, when present in marginal periodontitis-affected teeth, has recently been shown to be an aggravating factor in progression of marginal destruction. this has been associated with patency of dentinal tubules in the tooth cervix, an area normally devoid of cementum following periodontal therapy. these studies are, however hampered by that only circumstantial evidence such as presence of periapical destruction have been applied as criteria of endo ...19958625937
competition for peptides and amino acids among periodontal bacteria.we recently studied the utilization of glutathione (l-gamma-glutamyl-l-cysteinylglycine), l-cysteinylglycine and l-cysteine by anaerobic bacteria. the rate of hydrogen sulfide formation from these compounds was determined and it was concluded that peptostreptococcus micros and fusobacterium nucleatum subsp. nucleatum had an active transport of small peptides. in the present study it is shown that methyl mercaptan formation from l-methionine and l-methionyl-containing peptides can also be used to ...19958544102
endodontic pathogens in periodontal disease augmentation.periapical pathology indicating endodontic infection, when present in periodontitis-affected teeth, has recently been shown to be correlated to marginal periodontal breakdown. this has been associated with patency of dentinal tubules in the tooth cervix, an area normally devoid of cementum following periodontal therapy. these studies are, however hampered by that only circumstantial evidence such as presence of periapical destruction have been applied as criteria of endodontic infection. the aim ...19958583015
antimicrobial and toxic effects of established and potential root canal irrigants.three dilutions of the commonly used irrigant sodium hypochlorite (0.5%, 2.5%, and 5.25%) and the potential irrigants peridex, chlorhexidine gluconate (0.12%), and therasol were tested. alcohol and saline served as controls also. the in vitro portion of this study used four different microorganisms (streptococcus mutans, peptostreptococcus micros, prevotella intermedius, and porphyromonas gingivalis) to determine antimicrobial effect. presterilized 7-mm diameter whatman paper disks soaked with t ...19958596073
pet findings in a brain abscess associated with a silent atrial septal defect.brain abscesses are classical complications of congenital heart disease (chd) in children and adolescents. this association is rarely observed in adults. we report a 46-year-old man presenting a fronto-parietal abscess associated with an asymptomatic atrial septal defect. positron emission tomography (pet) study revealed high uptake of l-[methyl-11c]methionine ([11c]methionine) and 2-[18f]fluoro-2-deoxy-d-glucose (fdg) around the brain abscess. we suggest (1) to exclude a silent cardiac malforma ...19958599907
microbiological features and pathogenesis of peritonsillar abscesses.samples of pus aspirated from 53 peritonsillar abscesses were examined in detail for aerobic and anaerobic bacteria, and the microbiological results correlated with clinical data in 44 cases. in 45 samples (85%) cultures were positive: 7 yielded organisms consistent with an aerobic infection, mainly lancefield group a beta-haemolytic streptococci (5/7), and 38 yielded organisms consistent with an anaerobic infection. the anaerobic infections were usually mixed, but in two cases fusobacterium nec ...19958605900
bioactivation of cysteine conjugates of 1-nitropyrene oxides by cysteine conjugate beta-lyase purified from peptostreptococcus magnus.to determine the role of cysteine conjugate beta-lyase (beta-lyase) in the metabolism of mutagenic nitropolycyclic aromatic hydrocarbons, we determined the effect of beta-lyase on the mutagenicities and dna binding of cysteine conjugates of 4,5-epoxy-4,5-dihydro-1-nitropyrene (1-np 4,5-oxide) and 9,10-epoxy-9,10-dihydro-1-nitropyrene (1-np 9,10-oxide), which are detoxified metabolites of the mutagenic compound 1-nitropyrene. we purified beta-lyase from peptostreptococcus magnus gai0663, since p. ...19958526486
salivary levels of suspected periodontal pathogens in relation to periodontal status and treatment.the primary ecological niche for suspected periodontal pathogens seems to be the subgingival area, even though periodontal pathogens are also frequently recovered from saliva. the interrelationship of different periodontal conditions and the salivary levels of suspected periodontal pathogens is not known. in the present study, salivary levels of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, and peptostreptococcus micros were determin ...19958530742
structures of bacterial immunoglobulin-binding domains and their complexes with immunoglobulins.three-dimensional structures are now available for several immunoglobulin binding domains from bacterial proteins a, g, and l. x-ray diffraction and nmr experiments on complexes of these domains with portions of immunoglobulins have revealed common structural themes used in these interactions. these data expand our understanding of structure/function relationships in these molecular recognition processes and provide the basis for rational design of artificial immunoglobulin-binding molecules.19958528763
pneumonia in mechanically ventilated children.the quantitative aerobic and anaerobic microbiology of bronchial aspirates, obtained using protective brush catheters, from 10 children with ventilator-associated pneumonia, is presented. aerobic or faculative organisms only were isolated in 1 child, anaerobic bacteria only in 3, and aerobic mixed with anaerobic bacteria in 6. there were 10 aerobic or faculative and 17 anaerobic isolates. the predominant aerobes were pseudomonas aeruginosa (2 isolates) and klebsiella sp. (2). the predominant ana ...19958685643
[aerobic and anaerobic bacterial flora in acute abdominal illnesses].366 specinmens taken from patients suffering from acute abdominal illnesses who had undergone operations were examined. bacterial pathogens were isolated from 318 specimens. aerobic bacteria were isolated from 53.8% specimens. anaerobic bacteria were isolated from 12.6% specimens. mixed bacterial flora were isolated from 33.6% specimens. among aerobic bacteria e. coli and streptococcus faecalis were most often isolated. among anaerobic bacteria bacteroides fragilis, peptostreptococcus spp. and p ...19958833932
non-sporing anaerobes in certain surgical group of patients.the magnitude of non-sparing anaerobic (nsa) infections has been defined in the postoperative wounds on colorectum in children (57.1%), general surgery (0%), abdominoperineal and uterocervical operations (11-45%) in gynaecologic and obstetrical cases and perforative peritonitis (25.8-32.3%). children below the 6 months age group bear less risk of acquiring nsa infection. under certain situations, metronidazole combats nsa infections in a better way than other antibacterials. the bacteriology of ...19958648152
cloning, expression and purification of ppl-1, a kappa-chain binding protein, based upon protein l from peptostreptococcus magnus.protein l is a multi-domain, cell wall constituent of certain strains of peptostreptococcus magnus, which binds to the variable domain of the l-chains of ig. a gene fragment which codes for a single ig-binding domain of protein l (ppl-1) was cloned into a modified pkk223-3 vector and over-expressed in e. coli jm103. a rapid protein purification protocol is described. in these studies, purified ppl-1 was immobilised on to an agarose gel and tested against an array of igs and ig fragments. it was ...19958767928
crystallization and x-ray analysis of a single fab binding domain from protein l of peptostreptococcus magnus.protein l is a multidomain cell wall constituent of certain strains of peptostreptococcus magnus which binds to the variable domain of immunoglobulin kappa-light chains. a single immunoglobulin-binding domain of m(r) = 9000 from this protein has been isolated and crystallized. the crystals are of space group p4(2)2(1)2, with cell dimensions a = b = 66.9 a, c = 68.3 a, and diffract to at least 2.2 a resolution. the asymmetric unit of the crystal contains two molecules of the protein l domain, rel ...19958749861
a risk-benefit assessment of teicoplanin in the treatment of infections.teicoplanin is a glycopeptide antibiotic whose activity is selectively oriented against gram-positive aerobic and anaerobic bacteria, including staphylococcus aureus, coagulase-negative staphylococci, clostridium difficile, peptostreptococcus spp. and corynebacterium jeikeium; such activity is affected by neither methicillin resistance nor beta-lactamase production. teicoplanin is not significantly absorbed from the gastrointestinal tract; consequently, it has to be administered intravenously (e ...19958785019
oral microbiota in subjects with a weak or strong response in experimental gingivitis.the purpose of the present study was to examine the composition of the oral microbiota in subjects who had previously demonstrated to develop either a weak or strong response to experimental gingivitis. for this study, subjects were selected from a pool of 25 individuals who had participated twice in an experimental gingivitis trial. out of these 25 panellists, 6 subjects were selected who had developed 2x a weak gingival inflammatory response and 7 subjects who had developed 2x a strong gingiva ...19958583022
a study of the anaerobic bacterial flora of the female genital tract in health and disease.semi-quantitative and qualitative bacterial assessment of the vaginal and cervical flora of a total of 202 women was carried out over a period of six months to determine the bacterial flora in three groups of women and changes caused by prior use of antibiotics. the number was made up of 32 healthy volunteers, 80 women with gynaecological problems and 90 women with gynaecological infections who had had antibiotic treatment prior to this study. standard methods were used for the investigations. f ...19958907604
macrolides resistance of common bacteria isolated from taiwan.to determine the susceptibility to macrolides of common pathogenic bacteria isolated from taiwan, the in vitro activities of erythromycin, roxithromycin, azithromycin, clarithromycin, and dirithromycin were tested against 492 clinical isolates of eight different bacteria, collected from the national taiwan university hospital. the results showed high minimum inhibitory concentrations (mics) against most of the tested bacteria. the mic90s for staphylococcus aureus (both methicillin-resistant and ...19959407220
mapping of the immunoglobulin light chain-binding site of protein l.protein l is a cell surface protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus. the molecule binds specifically and with high affinity to immunoglobulins (ig) of a wide range of animal species. the ig-binding activity is mediated through five highly homologous domains, each 72 to 76 amino acid residues long, which interact with framework regions in the variable domain of ig light chains. the interaction does not interfere with the antigen binding capac ...19957608965
microbiologic and clinical study of polytetrafluoroethylene membranes for guided bone regeneration around implants.this study determined the microbiota of the mucosa- and implant-facing parts of expanded polytetrafluoro ethylene augmentation material, and the influence of major periodontopathogens on the healing process associated with guided bone regeneration around dental implants. seventeen patients with nine dehiscence and eight extraction defects were studied. prior to surgery and at membrane removal, microbial morphotypes, total viable counts, and the occurrence of selected microbial species were exami ...19957615319
the ga module, a mobile albumin-binding bacterial domain, adopts a three-helix-bundle structure.we present the first study of the secondary structure and global fold of an albumin-binding domain. our data show that the ga module from protein pab, an albumin-binding protein from the anaerobic bacterial species peptostreptococcus magnus, is composed of a left-handed three-helix bundle. the helical regions were identified by sequential and medium range noes, values of nh-c alpha h coupling constants, chemical shift indices, and the presence of slowly exchanging amide protons, as determined by ...19957589548
prosthetic-valve endocarditis caused by peptostreptococcus anaerobius. 19957620041
advanced periodontitis associated with larsen's syndrome: case report.larsen's syndrome is a genetic disorder of connective tissue characterized by multiple large-joint dislocations, abnormalities of hands and feet, and anomalous facial features. although findings involving multiple systems have been added to its clinical and pathological entity, oral pathology besides cleft palate has not been reported. we report here advanced periodontitis with severe gingival inflammation in a 29-year-old larsen's syndrome patient. bacterial culture of subgingival plaque identi ...19957899108
periodontal findings in spouses. a clinical, radiographic and microbiological study.clinical, radiographic and microbiological examination of periodontal conditions was carried out in 2 groups of married couples to assess similarities between husband and wife. the diseased probands (n = 10) exhibited advanced periodontitis and the healthy ones (n = 10) were periodontally normal. the clinical examination comprised the assessment of plaque, probing pocket depths, gingival bleeding on probing, suppuration, supragingival and subgingival calculus. the extent and type of alveolar bon ...19957775676
budgerigar breeders' hypersensitivity pneumonitis presenting as chronic bronchitis with purulent sputum.a 49-year-old woman complained of cough and a purulent sputum (about 40 ml/day). she kept budgerigars for a period of 30 years, she first noticed a productive cough 10 years previously. her chest roentgenogram revealed a diffuse reticulonodular pattern. she was diagnosed as having a chronic type of budgerigar breeder's lung associated with chronic bronchitis. peptostreptococcus anaerobius was cultured from her sputum, bronchoalveolar lavage fluid, as well as from droppings of her budgerigar. thi ...19957496084
[a case of gas-producing brain abscess with subdural empyema: timing of the operation for brain abscess].a case of gas-producing brain abscess with subdural abscess was reported. an 18-year-old boy was admitted with a five-day history of vomiting and high grade pyrexia. plain skull roentgenograms demonstrated left frontal multiple gas bubbles. ct scan and mri showed that both brain and subdural abscesses contained gas in the left frontal area. antibiotics and glyceol were intravenously administrated. in serial ct scans, subdural abscess was not recognized, while brain abscess was enhanced in a ring ...19957477705
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