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articulation index predictions of speech intelligibility in hearing aid selection. 20123401281
total spinal anaesthesia for a jehovah's witness with primary aldosteronism. 20123354822
colour blindness distribution in the male population of rome.a total of 3,285 young males selected at random from the school population of rome have been administered the ishihara plates for colour blindness. those who failed to read all plates correctly were further administered farnsworth's panel d-15 and the diagnoses of colour blindness were made by an ophthalmologist and cross checked. a total of 201 subjects were found to be colour-blind, allowing a gene frequency estimate of 0.061 +/- 0.004. this is the first reliable estimate for the italian popul ...20123489665
[etiopathogenesis of diabetes mellitus]. 20123299516
pharmaceutical and biomedical analysis meeting. weesp (the netherlands), november 13, 1986. abstracts. 20123353209
inhibitory effect of normal and cancerous tissues of the bladder on plasminogen activation. 20124270696
myelodysplastic syndromes: analysis of 131 cases according to the fab classification.the clinical and haematological findings in 131 patients with myelodysplastic syndromes (mds), none of which had previously received chemotherapy or radiotherapy, classified according to the fab criteria, were analysed. the distribution among the 5 subgroups was: ra 31 patients, ras 19, raeb 23, cmml 29 and raebt 29 patients. there were difficulties in the classification of 24 patients. these included, first, 8 cases with myeloid hyperplasia of the bone marrow (bm) but without monocytosis or exc ...20123609254
evaluation of an elisa for canine leishmaniasis immunodiagnostic using recombinant proteins.the present work describes the isolation and purification of two leishmania chagasi (= syn. leishmania infantum) recombinant proteins, rlci2b and rlci1a, and their use in the development of an immunoassay for the diagnostic of canine leishmaniasis. after protein expression and cell disruption, rlci2b was purified by immobilized metal affinity chromatography followed by size exclusion chromatography, whereas rlci1a, expressed as an inclusion body, was treated with urea and purified by anion-excha ...201221929686
contact with domestic dogs increases pathogen exposure in endangered african wild dogs (lycaon pictus).infectious diseases have contributed to the decline and local extinction of several wildlife species, including african wild dogs (lycaon pictus). mitigating such disease threats is challenging, partly because uncertainty about disease dynamics makes it difficult to identify the best management approaches. serious impacts on susceptible populations most frequently occur when generalist pathogens are maintained within populations of abundant (often domestic) "reservoir" hosts, and spill over into ...201222238695
a preliminary parasitological survey of hepatozoon spp. infection in dogs in mashhad, iran.we attempted to determine the prevalence of hepatozoon spp. infection in mashhad, northeast of iran, via blood smear parasitology.201223323098
multiple myeloma in a dog with multiple concurrent infectious diseases and persistent polyclonal gammopathy.a 12-year-old, spayed female, mixed-breed dog was presented for acute hematuria, stranguria, polyuria, and polydipsia, as well as lameness for 8 days. previous medical history included treatment for infection with ehrlichia canis, anaplasma phagocytophilum, leishmania infantum, and dirofilaria immitis 6.5 years prior to presentation. besides persistently increased antibody titers to e canis and a phagocytophilum, polyclonal gammopathy with a monoclonal spike and moderate hypercalcemia were obser ...201223278475
first description of natural ehrlichia canis and anaplasma platys infections in dogs from argentina.bacteria belonging to the anaplasmataceae family are vector transmitted agents that affect a variety of vertebrate hosts including the tick-borne pathogens ehrlichia canis and anaplasma platys, which cause canine monocytic ehrlichiosis and cyclic thrombocytopenia, respectively. these two infections, typically reported from tropical and sub-tropical regions, have not been previously reported in dogs from argentina. a total of 86 blood samples from dogs with suspected rickettsial disease and 28 no ...201223273677
zoonotic pathogens associated with hyalomma aegyptium in endangered tortoises: evidence for host-switching behaviour in ticks?hyalomma aegyptium is a hard-tick with a typical three-host life cycle. the main hosts are palearctic tortoises of genus testudo. however, other hosts can be used by immature ticks for feeding in natural conditions. given this complex ecology and multiple host use, the circulation of pathogens by h. aegyptium between various hosts can be important from epidemiological point of view. the aim of this study was to evaluate the role of h. aegyptium as natural carrier of four important zoonotic patho ...201223273169
effect of dead-sea brine and its main salts on cell growth in culture.diluted dead-sea brine and solutions of certain of its salts (chlorides and bromides of na, k and mg) were found to reversibly inhibit cell proliferation in culture. bromides were more powerful as inhibitors than their chloride counterparts, and k salts were more effective than those of na and mg. kbr had the strongest inhibitory effect, which equalled that of diluted dead-sea brine at the same concentration. the favourable results of the dead-sea spa treatment of psoriasis may thus be partly du ...20123432355
borrelia, rickettsia, and ehrlichia species in bat ticks, france, 2010.argas vespertilionis, an argasid tick associated with bats and bat habitats in europe, africa, and asia has been reported to bite humans; however, studies investigating the presence of vector-borne pathogens in these ticks are lacking. using molecular tools, we tested 5 a. vespertilionis ticks collected in 2010 from the floor of a bat-infested attic in southwestern france that had been converted into bedrooms. rickettsia sp. avbat, a new genotype of spotted fever group rickettsiae, was detected ...201223171714
haemoparasites of free-roaming dogs associated with several remote aboriginal communities in australia.tick-borne haemoparasites babesia vogeli and anaplasma platys are common among the free-roaming canine populations associated with aboriginal communities in australia, whilst the prevalence of haemoplasmas, which are also suspected to be tick-borne, remained unexplored. the aim of this study was to determine the prevalence of haemoplasma infection in these populations, and to identify any correlation with other haemoparasites. blood was collected from 39 dogs associated with four aboriginal comm ...201222584120
prevalence of dirofilaria immitis, ehrlichia canis, borrelia burgdorferi sensu lato, anaplasma spp. and leishmania infantum in apparently healthy and cvbd-suspect dogs in portugal--a national serological study.canine vector-borne diseases (cvbds) are caused by a wide range of pathogens transmitted to dogs by arthropods including ticks and insects. many cvbd-agents are of zoonotic concern, with dogs potentially serving as reservoirs and sentinels for human infections. the present study aimed at assessing the seroprevalence of infection with or exposure to dirofilaria immitis, ehrlichia canis, borrelia burgdorferi sensu lato, anaplasma spp. and leishmania infantum in dogs in portugal.201222452990
serological investigation of vector-borne disease in dogs from rural areas of china.to evaluate the anaplasma phagocytophilum (a. phagocytophilum), ehrlichia canis (e. canis), dirofilaria immitis (d. immitis) (canine heartworm), borrelia burgdorferi (b. burgdorferi) infections in countryside dogs from yunnan, hainan and anhui provinces.201223569877
when pain afferent neurons fire automatically. 20123327596
sickle cell anemia and cholelithiasis. 20123353155
comparison between 0.5% bupivacaine with and without glucose in lumbar epidural block for knee surgery.a double-blind study was performed in 41 patients (asa i) to compare 0.5% bupivacaine with and without 8% glucose in epidural analgesia. there was no difference in the onset, spread, duration or intensity of the epidural anaesthesias. the absorption of bupivacaine to circulation was similar in both groups. no major complications were noted and the anaesthesias were satisfactory for surgery in all patients.20123296606
who should treat diabetic retinopathy? 20123253127
[the t-lymphocyte subpopulation and reactivity to pwm in patients with rheumatoid arthritis]. 20123259067
[comparative study of the cosmetic effect of using a vertical pi-shaped skin suture with gauze rolls in abdominal operations]. 20123537478
detection of leishmania infantum, babesia canis, and rickettsiae in ticks removed from dogs living in italy.the aims of this study were to determine natural infections by anaplasma phagocytophilum/anaplasma platys, bartonella henselae, ehrlichia canis, leishmania infantum, rickettsia spp., babesia spp., and hepatozoon spp. by molecular methods in ticks (n=91) removed from dogs with clinical signs and laboratory abnormalities compatible with tick-borne diseases (n=22) living in italy and to assess the distribution and species of ticks encountered. ticks from dogs living in southern italy were all ident ...201223182545
seroprevalence of tick-borne infections in military working dogs in the republic of korea.in this study we endeavored to determine the seroprevalence of tick-borne infections in the military working dog (mwd) population in the republic of korea (rok). our sample population consisted of 182 serum samples from mwds for 3 different years (1996, 2002, and 2007). in addition, 63 whole blood samples from 2007 were available for polymerase chain reaction (pcr). serum samples were evaluated by a commercially available enzyme-linked immunosorbent assay (elisa) and immunofluorescence assay (if ...201223167501
prevalence of antibodies against rickettsia conorii, babesia canis, ehrlichia canis, and anaplasma phagocytophilum antigens in dogs from the stretto di messina area (italy).the aims of this study were to determine the seroprevalence for rickettsia conorii, ehrlichia canis, anaplasma phagocytophilum, and babesia canis in outdoor-kennelled dogs (n=249) from the stretto di messina (italy) and to compare seroprevalence in 2 public shelters and 4 privately-owned kennels where different tick-preventive measures were implemented in order to focus on the specific sanitary risk posed by public shelters in southern italy for tick-borne pathogens. r. conorii (72%) and b. cani ...201223140895
migratory and carnivorous birds in brazil: reservoirs for anaplasma and ehrlichia species?in order to investigate new hosts for anaplasmataceae agents in brazil, we collected blood samples from 21 wild birds. using molecular techniques, we detected the presence of anaplasma phagocytophilum, ehrlichia chaffeensis, and an ehrlichia species closely related to ehrlichia canis in carnivorous avian blood samples. in addition, an ehrlichia species closely related to an ehrlichia species found in wild felines in brazil was also detected in a goose blood sample. wild birds may play a role as ...201222607070
seroprevalence and geographic distribution of dirofilaria immitis and tick-borne infections (anaplasma phagocytophilum, borrelia burgdorferi sensu lato, and ehrlichia canis) in dogs from romania.tick-borne diseases are of great concern worldwide. despite this, in romania there is only limited information regarding the prevalence of vector-borne pathogens in dogs. in all, 1146 serum samples were tested by snap(®) 4dx(®) (idexx laboratories, inc., westbrook, me) for anaplasma phagocytophilum, borrelia burgdorferi, and ehrlichia canis antibodies, and for dirofilaria immitis antigen. the correlation between positive cases and their geographic distribution, as well as potential risk factors ...201222607068
a survey of ixodid ticks feeding on cattle and prevalence of tick-borne pathogens in the black sea region of turkey.the study reports the frequency of infestation and the prevalence of tick-borne pathogens in feeding adult ticks detached from cattle in two climatic zones of the black sea region of turkey. a total of 2160 adult ticks were collected during 2007-2008. of these, 1062 were randomly selected, divided into 224 pools, and tested for the presence of bovine theileria, babesia, and anaplasma species. eleven tick species were recognized on cattle in the study. hyalomma marginatum was widely disrubuted in ...201222365338
acaricidal activity of ethanolic extract from aerial parts of tagetes patula l. (asteraceae) against larvae and engorged adult females of rhipicephalus sanguineus (latreille, 1806).the tick rhipicephalus sanguineus is the species with the largest worldwide distribution and is proven to be involved in the transmission of pathogens such as babesia canis, ehrlichia canis, coxiella burnetii, rickettsia ricketsii, rickettsia conorii, among others. studies have demonstrated acquisition of resistance to some of the active principles used in commercial formulations of acaricides. tagetes patula (asteraceae) is a plant with highlighted economic and commercial importance due to the ...201223244493
postanesthesia recovery scores and postoperative hypoxemia. 20123421487
seroprevalence of ehrlichia canis, ehrlichia chaffeensis and ehrlichia ewingii in dogs in north america.this study evaluated the exposure of dogs to three different ehrlichia spp. in the south and central regions of the united states where vector-borne disease prevalence has been previously difficult to ascertain, particularly beyond the metropolitan areas.201222316160
an assessment of whole blood and fractions by nested pcr as a dna source for diagnosing canine ehrlichiosis and anaplasmosis.ehrlichiosis and anaplasmosis are tick-borne diseases. ehrlichia canis and anaplasma platys infect mainly white cells and platelets, respectively. the main dna source for pcr is peripheral blood, but the potential of blood cell fractions has not been extensively investigated. this study aims at assessment of whole blood (wb) and blood fractions potential in nested pcr (npcr) to diagnose canine ehrlichiosis and anaplasmosis. the 16s rrna gene was amplified in 71.4, 17.8, 31.57, and 30% of the wb, ...201222973174
first molecular detection of ehrlichia canis and anaplasma platys in ticks from dogs in cebu, philippines.ehrlichia canis infection of dogs in the philippines has been detected by serological and peripheral blood smear examination methods, but not by molecular means. anaplasma platys infection in dogs has not yet been officially reported, although it is suspected to occur in the country. thus, sensitive and specific molecular techniques were used in this study to demonstrate the presence of both e. canis and a. platys in the philippines. a total of 164 rhipicephalus sanguineus ticks was collected fr ...201223168049
perinuclear antineutrophil cytoplasmic autoantibodies in dogs infected with various vector-borne pathogens and in dogs with immune-mediated hemolytic anemia.to determine the prevalence of perinuclear antineutrophil cytoplasmic autoantibodies (panca) in dogs with confirmed or suspected immune-mediated hemolytic anemia (imha) or dogs infected with various vector-borne pathogens, including rickettsia rickettsii, bartonella henselae, bartonella vinsonii subsp berkhoffii, ehrlichia canis, borrelia burgdorferi, and leishmania infantum.201222924722
the comparison of spatial variation and risk factors between mosquito-borne and tick-borne diseases: seroepidemiology of ehrlichia canis, anaplasma species, and dirofilaria immitis in dogs.canine ehrlichiosis/anaplasmosis and heartworm diseases are vector-borne and zoonotic infections. to compare epidemiology of these vector-borne diseases, a community-based study was conducted to determine the seroprevalence and risk factors of ehrlichia canis, anaplasma sp. and dirofilaria immitis infections among healthy pet dogs. prevalence distribution patterns were geographically contrasting between tick-borne e. canis/anaplasma sp. infections and mosquito-borne d. immitis infection. althoug ...201222925931
mitochondrial dna analysis of rhipicephalus sanguineus sensu lato (acari: ixodidae) in the southern cone of south america.a genetic analysis of partial sequences of the mitochondrial 16s and 12s rdna genes of rhipicephalus sanguineus sensu lato collected in the southern cone of south america was carried out. also, sequences of ticks belonging to this taxon from europe, africa and other south american localities were included. tcs networks constructed with 16s rdna sequences showed two clusters of haplotypes, namely, southern lineage (st) and northern lineage (nt). haplotypes representing the specimens coming from l ...201222818199
haematological parameters in stray dogs seropositive and seronegative to ehrlichia canis in north trinidad.in view of the fact that stray dogs are a reservoir for many diseases, this study was undertaken to determine the prevalence of ehrlichia canis in stray dogs in north trinidad and to evaluate the diagnostic implications of haematological alterations associated with seropositivity. overall, 41 (44.6%) of 92 stray dogs were seropositive to e. canis by the indirect immunofluorescent antibody test. dogs, one year of age and older (59.7%) were more likely to be seropositive than dogs less than one ye ...201222658916
pulmonary hypertension associated with ehrlichia canis infection in a dog. 201222645155
serum cardiac troponin i concentrations in naturally occurring myelosuppressive and non-myelosuppressive canine monocytic ehrlichiosis.the aim of this study was to determine whether serum cardiac troponin i (ctni) concentration measured on admission was associated with the clinical severity of canine monocytic ehrlichiosis and was predictive of clinical outcome (death or survival) in dogs naturally infected with ehrlichia canis. serum ctni concentration was compared among 22 dogs with non-myelosuppressive ehrlichiosis (nme), 22 dogs with myelosuppressive ehrlichiosis (me) and 10 healthy dogs. unlike healthy dogs, 45.5% nme and ...201222633176
direct detection of ehrlichia canis by pcr in the conjunctiva of a dog with bilateral anterior uveitis.the following report describes the direct detection of ehrlichia canis by real-time pcr in the conjunctiva of a 1-year-old female maltese dog. after being imported from brazil, the dog was presented because of anorexia, dehydration, fever, and palpable mandibular lymph nodes. a few days later, the dog developed bilateral blepharospasm, photophobia and anterior uveitis. monocytic ehrlichia was diagnosed by a positive pcr result and the detection of igm and igg antibodies. because of the massive u ...201222563611
evaluation of peripheral blood lymphocyte subsets in family-owned dogs naturally infected by ehrlichia canis.previous research suggested that clinical manifestations, histopathological lesions, and even infection maintenance in the course of canine monocytic ehrlichiosis (cme) are directly related to the immune response developed by the host. in the present study, blood lymphocyte subsets were analyzed by multiparametric flow cytometry in 37 dogs with naturally occurring cme and 47 healthy dogs used as controls. t, t helper (th), t cytotoxic (tc), b, non-t, non-b lymphocytes and those that express mhc ...201222521727
the role of cytoskeleton, components of inositol phospholipid signaling pathway and iron in ehrlichia canis in vitro proliferation.ehrlichia canis, etiologic agent of canine monocytic ehrlichiosis, is an obligatory intracellular bacterium that parasitizes monocytes and macrophages. in this study we analyzed the role of the cytoskeleton specifically actin microfilaments and microtubules, components of inositol phospholipid signaling pathway such as phospholipase c (plc), protein kinase (ptk) and calcium channels as well as the role of iron in the e. canis proliferation in dh82 cells. different inhibitory compounds were used ...201222415481
ehrlichia canis infection in two dogs that emigrated from endemic areas.two dogs, emigrated from zambia and china to japan, were diagnosed with ehrlichia canis infection. both cases had thrombocytopenia, non-regenerative anemia, and hypergloblinemia with polyclonal gammopathy. case 1 had ataxia of the hind limbs. severe meningitis was revealed by magnetic resonance imaging examination. intracytoplasmic inclusions were observed in mononuclear cells of cerebrospinal fluid. case 2 had a history of bilateral epistaxis, and severe pancytopenia was noticed in complete blo ...201222214859
[molecular identification of ehrlichia canis in a dog from arica, chile].we report a molecular confirmed case of canine ehrlichiosis caused by ehrlichia canis. a 10-year old female crossbred siberian from the city of arica, which was infested by ticks, presented hemorrhagic manifestations (hematomas and snout bleeding) and prostration. blood cell count revealed thrombocytopenia (30,000 platelets/ mm³). immunochromatographic rapid testing for e. canis igg was positive. amplification and sequencing of a fragment of the 16s rrna gen from a blood sample showed 100% homol ...201223282495
ehrlichia canis in rhipicephalus sanguineus ticks in the ivory coast.canine monocytic ehrlichiosis caused by ehrlichia canis is distributed globally, but its prevalence in africa is poorly known. in the study reported herein, 27% of rhipicephalus sanguineus ticks collected from watchdogs in abidjan, ivory coast, were positive for e. canis using quantitative real-time pcr. a new molecular strategy is proposed that can be used not only for epidemiological study, but also for the diagnosis of canine monocytic ehrlichiosis. our findings show for the first time the pr ...201223141106
first detection of ehrlichia canis in rhipicephalus bursa ticks in sardinia, italy.canine monocytic ehrlichiosis is a common and widespread disease. this disease is present in sardinia year-round because the temperate weather on the island permits the survival of many types of tick vectors. a total of 35 ticks was collected from goats in south-eastern sardinia, italy, and tested for ehrlichia dna using polymerase chain reaction and dna sequencing. this study provides the first evidence of the presence of ehrlichia canis in rhipicephalus bursa ticks. the data presented here inc ...201223140896
in silico characterization of three two-component systems of ehrlichia canis and evaluation of a natural plant-derived inhibitor.two-component signal transduction systems (tcs) are important elements in the interaction of endobacteria with host cells. they are basically composed of two proteins, an environmental signal sensor and a response regulator, which activate genes involved in a wide range of bacterial responses to their environment. we analyzed three sets of genes corresponding to tcs of ehrlichia canis, a common tick-borne canine pathogen and the etiologic agent of canine monocytic ehrlichiosis, in order to ident ...201223096683
evaluation of an attenuated strain of ehrlichia canis as a vaccine for canine monocytic ehrlichiosis.canine monocytic ehrlichiosis is an important tick-borne disease worldwide. no commercial vaccine for the disease is currently available and tick control is the main preventive measure against the disease. the aim of this study was to evaluate the potential of a multi-passaged attenuated strain of ehrlichia canis to serve as a vaccine for canine monocytic ehrlichiosis, and to assess the use of azithromycin in the treatment of acute ehrlichiosis. twelve beagle dogs were divided into 3 groups of 4 ...201223072894
experimental ehrlichia canis infection changes acute-phase proteins.early diagnosis of canine ehrlichiosis favors prompt institution of treatment and improves the prognosis for the animal, since this disease causes mortality among dogs. studies have shown that determining the concentration of acute-phase proteins (apps) may contribute towards early detection of disease and aid in predicting the prognosis. this study aimed to evaluate the app profile in dogs experimentally infected with ehrlichia canis, at the start of the infection and after treatment. it also i ...201223070428
evaluation of lymphocyte populations in dogs naturally infected by ehrlichia canis with and without clinical signs.immune response elicited by the host during ehrlichial infections could influence the clinical signs and laboratory and pathological findings. twenty-eight dogs naturally infected by ehrlichia canis were included in this study. twenty of them presented only laboratory findings traditionally associated with canine monocytic ehrlichiosis (cme), whilst 8 dogs also showed clinical signs classically associated with cme (pale mucous membranes, fever, lymphadenopathy, weight loss, anorexia, lethargy or ...201223168046
ehrlichia canis in dogs in a semiarid region of northeastern brazil: serology, molecular detection and associated factors.this study investigated infection by ehrlichia spp. agents by pcr, immunofluorescence assay test (ifat), and by giemsa-stained blood smears in 108 dogs from a semiarid region of the state of paraíba, northeastern brazil. seventy-five (69.4%) of the 108 dogs were found to be seropositive to ehrlichia canis, while only four dogs (3.7%) were positive in real-time pcr for e. canis. in six dogs (5.6%) e. canis-like morulae were observed in monocytes. animals that stayed in environment whose floor was ...201323141416
first detection and molecular characterization of ehrlichia canis from dogs in nigeria.the present study aimed to detect the presence of ehrlichia canis in naturally infected dogs in nigeria, using a combination of pcr and sequence analysis of the 16s rrna gene and two genes encoding the tandem repeat-containing proteins (trps), trp19 and trp36. out of a total of 100 blood samples collected from domestic dogs presented to veterinary hospitals in jos, the capital city of plateau state of nigeria, 11 were positive in nested pcr for e. canis. sequencing results for these amplicons sh ...201322925936
serological survey on ehrlichia sp. among dogs in the central region of rio grande do sul.a serological survey on ehrlichia canis was conducted among dogs in the central area of the state of rio grande do sul, where the tick rhipicephalus sanguineus is a common parasite of dogs. out of a total of 316 dogs attended at the veterinary teaching hospital in the municipality of santa maria, only 14 (4.43%) reacted positively to e. canis antigens in the indirect immunofluorescence assay, with the following endpoint titers: 80 (three dogs), 160 (five), 320 (four), 640 (one) and 1280 (one). l ...201323184320
in vitro culture of a novel genotype of ehrlichia sp. from brazil.ehrlichiae are obligate intracytoplasmic gram-negative, tick-borne bacteria belonging to the anaplasmataceae family. ehrlichioses are considered emerging diseases in both humans and animals. several members of the genus ehrlichia have been isolated and propagated in vitro. this study describes the continuous propagation of a brazilian ehrlichia sp. isolate in ide8 tick cells, canine dh82 cells and bovine aorta cells. initially, the organisms were isolated from the haemolymph of a rhipicephalus ( ...201324589106
inhibitory effect of interferon gamma on frequency of ehrlichia canis-infected cells in vitro.ehrlichia canis is an obligate intracellular bacterium that infects the macrophage-monocyte cells of dogs, causing canine monocytic ehrlichiosis. interferon-γ (ifn-γ), along with other cytokines, mediates the immune response to such intracellular bacterial invasions. to determine the role of ifn-γ in the immunity of dogs to e. canis infection, peripheral blood mononuclear cells (pbmc) and white blood cells (wbc) were collected from e. canis-infected dogs and added to a culture of e. canis in dh8 ...201324148826
ultrastructure of ehrlichia mineirensis, a new member of the ehrlichia genus.recently, we reported the in vitro isolation and the molecular characterization of a new species of ehrlichia (ehrlichia mineirensis) from haemolymph of brazilian rhipicephalus (boophilus) microplus ticks. this organism shows an ortholog of ehrlichia canis major immunogenic protein gp36 with a new structure of tandem repeats. in the present study, we used electron microscopy (high pressure freezing and freeze substitution preparative techniques) to characterize morphologically this new agent gro ...201323998429
transmission of ehrlichia canis by rhipicephalus sanguineus ticks feeding on dogs and on artificial membranes.a south african strain of ehrlichia canis was isolated and used to infect a laboratory-bred beagle dog. rhipicephalus sanguineus nymphs, which fed on this dog, moulted to adult ticks which carried infection rates of e. canis between 12% and 19% and were used in a series of in vivo and in vitro experiments. five groups of 6 dogs were challenged with the infected r. sanguineus ticks, which were removed 24h, 12h, 6h or 3h after the ticks had been released onto the dogs. the animals were monitored f ...201323962826
influence of experimental canine ehrlichiosis on the e-ada activity and purine levels in serum and possible functional correlations with pathogenesis.the aim of this study was to evaluate adenosine deaminase activity and purines levels in serum of dogs experimentally infected by ehrlichia canis. banked serum samples of dogs divided into two groups with five animals each: healthy animals and animals infected by e. canis. the concentration of purines (adenosine triphosphate (atp), adenosine diphosphate (adp), adenosine monophosphate (amp), adenosine, inosine, hypoxanthine, xanthine and uric acid), and adenosine deaminase (e-ada) activity in ser ...201323907060
imidacloprid 10 % / flumethrin 4.5 % collars (seresto®, bayer) successfully prevent long-term transmission of ehrlichia canis by infected rhipicephalus sanguineus ticks to dogs.the objective of this study was to determine the empirical efficacy of imidacloprid 10 %/flumethrin 4.5 % (seresto®) collars in preventing long-term transmission of ehrlichia canis by infected rhipicephalus sanguineus ticks to dogs. the study was a parallel group design, single centre, randomised, non-blinded, controlled, long-term efficacy study. the treatment group of 8 dogs was fitted with seresto® collars, the untreated control group of 8 dogs received no collars. ehrlichia canis-infected ti ...201323774841
ehrlichia canis infection in a dog with no history of travel outside the united kingdom.a two-year-old female neutered tibetan terrier was referred following a one-month history of lethargy, inappetence and pancytopenia, which had been poorly responsive to immunosuppressive and fluoroquinolone treatment. the dog was diagnosed with pure red cell aplasia and was found to be positive for ehrlichia canis by both antibody titre measurement and polymerase chain reaction. the dog lived in london and had not travelled outside the uk. the dog was treated with doxycycline, prednisolone and c ...201323718904
increase nitric oxide and oxidative stress in dogs experimentally infected by ehrlichia canis: effect on the pathogenesis of the disease.the aim of this study was to evaluate nitric oxide levels, lipid peroxidation, protein oxidation and glutathione reductase activity in serum of dogs experimentally infected by ehrlichia canis. banked serum samples of dogs divided into two groups were used: negative control (n=5) and infected by e. canis (n=5). the concentration of nitrite/nitrate (nox), lipid peroxidation (tbars), advanced oxidation protein products (aopp), and glutathione reductase (gr) activity in sera were evaluated. samples ...201323540584
molecular detection of ehrlichia canis in dogs from the pantanal of mato grosso state, brazil.the present study evaluated the presence of ehrlichia dna in the blood samples of 320 dogs from the urban and rural areas of the municipality of poconé, pantanal region, mato grosso state, by polymerase chain reaction (pcr), targeting the ehrlichial dsb gene. risk factors for infection in dogs were also evaluated. forty-eight (15%, 95% ci: 11.4-19.5%) dogs were positive: 25 (15.6%, 95% ci: 10.4-22.2%) from the urban area and 23 (14.4%, 95% ci: 9.3-20.8%) from the rural area (p > 0.05). partial d ...201323538496
genetic diversity of ehrlichia canis in brazil.canine monocytic ehrlichiosis is a highly prevalent disease in brazil, where the genetic diversity of ehrlichia canis remains undefined. in this study, we used the trp36 gene to examine the genetic diversity of e. canis strains from naturally infected dogs residing in five distinct geographic regions in brazil. e. canis dna was detected in 82/126 (65%) dogs by dsb-specific pcr and e. canis was isolated in cell culture from 13 dogs. sequences obtained from dsb genes amplified from the isolates we ...201323490559
efficacy of rifampicin in the treatment of experimental acute canine monocytic ehrlichiosis.to assess the efficacy of rifampicin in achieving clinical and haematological recovery and clearing infection in dogs with experimentally induced acute monocytic ehrlichiosis.201323475646
prevention of transmission of ehrlichia canis by rhipicephalus sanguineus ticks to dogs treated with a combination of fipronil, amitraz and (s)-methoprene (certifect®).the ability of certifect(®) (a combination of fipronil, amitraz and (s)-methoprene) to prevent transmission of ehrlichia canis was studied in two groups of eight dogs. one group was treated with certifect while the other group remained untreated. all dogs were exposed to e. canis-infected rhipicephalus sanguineus ticks on days 7, 14, 21 and again on day 28 post-treatment by releasing ticks into the kennels of the dogs to simulate the natural way of infestation. animals were examined in situ for ...201323298566
molecular evidence of ehrlichia canis and rickettsia massiliae in ixodid ticks of carnivores from south hungary.to monitor the emergence of thermophilic, mediterranean ixodid tick species and tick-borne pathogens in southern hungary, 348 ticks were collected from shepherd dogs, red foxes and golden jackals during the summer of 2011. golden jackals shared tick species with both the dog and the red fox in the region. dermacentor nymphs were collected exclusively from dogs, and the sequence identification of these ticks indicated that dogs are preferred hosts of both d. reticulatus and d. marginatus nymphs, ...201323439290
mimicry epitope from ehrlichia canis for interphotoreceptor retinoid-binding protein 201-216 prevents autoimmune uveoretinitis by acting as altered peptide ligand.we report here identification of novel mimicry epitopes for interphotoreceptor retinoid-binding protein (irbp) 201-216, a candidate ocular antigen that causes experimental autoimmune uveoretinitis (eau) in a/j mice. one mimicry epitope from ehrlichia canis (ehc), designated ehc 44-59, induced cross-reactive t cells for irbp 201-216 capable of producing t helper (th)1 and th17 cytokines, but failed to induce eau in a/j mice. in addition, animals first primed with suboptimal doses of irbp 201-216 ...201324029580
are vector-borne pathogen co-infections complicating the clinical presentation in dogs?infection by two or more canine vector-borne disease (cvbd)-causing pathogens is common in subtropical and tropical regions where vectors are plentiful. co-infections may potentiate disease pathogenesis, thereby altering clinical manifestations typically associated with singular infections. these factors complicate diagnosis, treatment and can adversely influence prognosis if the practitioner fails to suspect, document, and treat each concurrent infection. the spectrum of pathogens co-infecting ...201323587324
evaluation of a prototype flow cytometry test for serodiagnosis of canine visceral leishmaniasis.diagnosing canine visceral leishmaniasis (cvl) is a critical challenge since conventional immunoserological tests still present some deficiencies. the current study evaluated a prototype flow cytometry serology test, using antigens and fluorescent antibodies that had been stored for 1 year at 4°c, on a broad range of serum samples. noninfected control dogs and leishmania infantum-infected dogs were tested, and the prototype test showed excellent performance in differentiating these groups with h ...201324108778
serosurvey for tick-borne diseases in dogs from the eastern amazon, brazil.canine ehrlichiosis and babesiosis are the most prevalent tick-borne diseases in brazilian dogs. few studies have focused attention in surveying tick-borne diseases in the brazilian amazon region. a total of 129 blood samples were collected from dogs living in the brazilian eastern amazon. seventy-two samples from dogs from rural areas of 19 municipalities and 57 samples from urban stray dogs from santarém municipality were collected. serum samples were submitted to indirect immunofluorescence a ...201323802236
arthropod-borne infections in travelled dogs in europe.pet animal movement is ever increasing within the european union and in that context canine vectorborne infections gained a considerable importance. information on these infections in travelled dogs is nevertheless limited. a first prospective study on vector-borne infections was conducted in 106 dogs travelling from germany to countries in south and south-east europe. the dogs were screened prior to and consecutively up to three times after travel by haematological (giemsa-stained buffy coat sm ...201323477298
identification of babesia species infecting dogs using reverse line blot hybridization for six canine piroplasms, and evaluation of co-infection by other vector-borne pathogens.canine infection by vector-borne hemoparasites is frequent in tropical and sub-tropical areas where exposure to hematophageous ectoparasites is intensive. a reverse line blot (rlb) assay was designed to improve the simultaneous detection of all named canine piroplasm species combined with other vector-borne pathogens of dogs including ehrlichia canis, hepatozoon canis and leishmania infantum common in the mediterranean basin. blood samples of 110 dogs from spain (n=21), portugal (n=14) and israe ...201323017370
mortality in virulent canine babesiosis is associated with a consumptive coagulopathy.the inflammatory response to infection can activate the coagulation system via complex interactions. if uncontrolled, this may lead to a consumptive coagulopathy, a major risk factor for a poor clinical outcome. this prospective observational study was conducted to determine whether consumptive coagulopathy in dogs with babesia rossi infection is related to mortality. seventy-two client-owned dogs diagnosed with canine babesiosis were included. diagnosis was confirmed by polymerase chain reactio ...201323098634
molecular detection of ehrlichia canis and anaplasma platys in dogs in southern brazil.the aims of this study were to determine the occurrence of anaplasma platys and ehrlichia canis infection in dogs in porto alegre, southern brazil; and to investigate their association with hematological abnormalities. serum samples from 196 dogs were first tested using dot-elisa for antibodies against anaplasma spp. and ehrlichia canis. peripheral blood samples from 199 dogs were subjected to 16s rrna nested pcr (npcr) for a. platys and e. canis, followed by dna sequencing to ensure pathogen id ...201324142166
babesia canis vogeli, ehrlichia canis, and anaplasma platys infection in a dog.a 12-month-old male neutered mixed breed dog was presented with a history of diarrhea, lethargy, emaciation, polydypsia, and sniffling. physical examination findings included pale mucous membranes, increased heart and respiratory rates, and normal rectal temperature (38°c). hematologic abnormalities included anemia and thrombocytopenia. biochemical abnormalities included hypoalbuminemia, hyperbilirubinemia, and elevated alp and alt activities. a snap 4dx test result was positive for ehrlichia ca ...201324138512
detection of ehrlichia canis and anaplasma platys dna using multiplex pcr.we hereby propose a novel sensitive, specific, and cost-efficient method to detect ehrlichia canis and anaplasma platys dna from canine whole blood samples by multiplex pcr. blood samples from hemoparasited dogs attending the veterinary hospital at the universidade federal rural da amazônia-ufra, belém, brazil, were collected in tubes containing edta. amplification of e. canis and a. platys 16s rdna by nested (n) pcr was successfully achieved by using primers specific to the anaplasmataceae in t ...201324107211
study of ehrlichiosis in kennel dogs under treatment and prevention during seven months in dakar (senegal).in dakar kennels where morbidity and mortality attributed to diseases transmitted by ticks were high, we conducted a field study to assess the prevalence of ehrlichia canis, anaplasma platys and babesia spp. infections in two kennels (n = 34 dogs) and to study the impact of tick protection. the first day of the study, the e. canis pcr were positive in 18 dogs (53%). a. platys was found in one dog and all dogs were negative for babesia spp. after one month of doxycycline treatment, the number of ...201324018180
chemical characterization and acaricide potential of essential oil from aerial parts of tagetes patula l. (asteraceae) against engorged adult females of rhipicephalus sanguineus (latreille, 1806).rhipicephalus sanguineus, commonly known as the brown dog tick, is one of the most widely distributed species of tick. in dogs, it can cause anemia and provide the transmission of pathogenic microorganisms such as babesia canis, ehrlichia canis, hepatozoon canis, anaplasma platys, and mycoplasma haemocanis. to man, it can transmit the intracellular parasites rickettsia rickettsii and rickettsia conorii, the causative agents of the rocky mountain spotted fever in the americas and mediterranean an ...201323508761
tick-borne pathogens and disease in dogs on st. kitts, west indies.between 2009 and 2011, we conducted a case-control study of ticks and tick-associated pathogens affecting dogs on the island of st. kitts, eastern caribbean, including 55 cases of clinically suspected tick-borne disease (tbd) and 110 presumably healthy animals presented for elective surgeries. rhipicephalus sanguineus caused year-round infestations of dogs, and 36% of the dogs in the study were infested at the time of examination. overall, 62% of suspected tbd cases and 24% of presumably healthy ...201323481028
occurrence of ehrlichia canis and anaplasma platys in household dogs from northern parana.canine monocytic ehrlichiosis caused primarily by ehrlichia canis and canine thrombocytic anaplasmosis induced by anaplasma platys are important emerging zoonotic tick-borne diseases of dogs. there is evidence that these pathogens can also affect humans. this study evaluated the presence of e. canis and a. platys in blood samples collected from 256 domiciled dogs in the municipality of jataizinho, located in north region of the state of parana, brazil, by pcr assay. the occurrence of e. canis an ...201323207986
serosurvey of tick-borne pathogens in dogs from urban and rural areas from parana state, brazil.considering the zoonotic potential of tick-borne disease (tbd) agents and the fact that dogs may act as sentinels for human infection, the aim of the present study was to determine the seroprevalence of tbd agents and risk factors for exposure in two different canine populations from parana state, southern brazil. a total of 138 dog serum samples from urban (ua) (n=68) and rural (ra) (n=70) areas were tested with commercial elisa rapid test for anaplasma phagocytophilum, ehrlichia canis and borr ...201324252955
prevalence of borreliosis, anaplasmosis, ehrlichiosis and dirofilaria immitis in dogs and vectors in voronezh reserve (russia).most of the dogs studied for the prevalence of cvbd have previously received acaricidal and insecticidal treatments. in the present work, a very specific population of dogs (group 1) that had never been treated against ticks and mosquitoes was studied. moreover, the territory occupied by this population has also never been treated, because it is a protected area--voronezh natural reserve. canine patients from veterinary clinics (group 2) that had been treated against vbd vectors were studied for ...201324054985
vector-borne disease surveillance in puerto rico: pathogen prevalence rates in canines ? implications for public health and the u.s. military ? applying the one health concept.vector-borne diseases (vbds) make up a large number of emerging infectious and zoonotic diseases. vectors such as ticks, fleas, and mosquitoes parasitize dogs, thus making canine populations adequate reservoirs for infectious disease and zoonoses. the u.s. military deploys its personnel and military working dogs (mwds) throughout the world with possible risk of exposure to vbds. canine vbds continue to have veterinary and public health significance for the host nations as well as for deployed u. ...201323817880
prevalence of antibodies to spotted fever group rickettsia spp. and ehrlichia spp. in coyotes (canis latrans) in oklahoma and texas, usa.coyotes (canis latrans) are commonly infested with ticks, including amblyomma americanum, the predominant vector of ehrlichia chaffeensis and ehrlichia ewingii; dermacentor variabilis, an important vector of rickettsia rickettsii; and amblyomma maculatum, a major vector of rickettsia parkeri, a spotted fever group (sfg) rickettsia. to determine the degree to which coyotes are infected with or exposed to tick-borne bacterial disease agents, serum samples collected from coyotes in oklahoma and tex ...201323778619
molecular evidence for bacterial and protozoan pathogens in hard ticks from romania.the aim of the present study was to provide a preliminary insight into the diversity of tick-borne pathogens circulating at the domestic host-tick interface in romania. for this, feeding and questing ticks were analyzed by real-time polymerase chain reaction (pcr) for the presence of anaplasma phagocytophilum, anaplasma platys, ehrlichia canis, borrelia burgdorferi sensu latu, and by pcr and subsequent sequencing for rickettsia spp., babesia spp. and theileria spp. a total of 382 ticks, encompas ...201323428204
molecular detection and characterization of tick-borne pathogens in dogs and ticks from nigeria.only limited information is currently available on the prevalence of vector borne and zoonotic pathogens in dogs and ticks in nigeria. the aim of this study was to use molecular techniques to detect and characterize vector borne pathogens in dogs and ticks from nigeria.201323505591
molecular detection of ehrlichia canis in dogs in malaysia.an epidemiological study of ehrlichia canis infection in dogs in peninsular malaysia was carried out using molecular detection techniques. a total of 500 canine blood samples were collected from veterinary clinics and dog shelters. molecular screening by polymerase chain reaction (pcr) was performed using genus-specific primers followed by pcr using e. canis species-specific primers. ten out of 500 dogs were positive for e. canis. a phylogenetic analysis of the e. canis malaysia strain showed th ...201323301114
the efficacy of advantix® to prevent transmission of ehrlichia canis to dogs by rhipicephalus sanguineus ticks.the capacity of a topical combination of imidacloprid and permethrin (advantix(®)) to prevent transmission of ehrlichia canis was studied in two groups of six dogs. one group served as controls, whereas the other group was treated. all dogs were exposed to e. canis-infected rhipicephalus sanguineus ticks on days 7, 14, 21 and day 28 post acaricidal treatment. the adult r. sanguineus ticks were released into the individual kennels of the dogs to simulate natural tick exposure. in situ tick counts ...201324135158
isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an ehrlichia canis strain from southeastern brazil.amplification of the 16s rrna gene from a blood sample obtained from a dog in southeastern brazil was used to confirm a naturally acquired ehrlichia (e.) canis infection. following isolation and culturing of the new bacterial strain called uberlandia, partial sequences of the dsb and p28 genes were obtained. the dsb partial sequence of the novel strain was 100% similar to dsb gene sequences of e. canis obtained from different geographic areas around the world. conversely, the p28 partial sequenc ...201324136211
frequency and clinical epidemiology of canine monocytic ehrlichiosis in dogs infested with ticks from sinaloa, mexico.ehrlichia canis is a rickettsial intracellular obligate bacterial pathogen and agent of canine monocytic ehrlichiosis. the prevalence of this disease in veterinary medicine can vary depending on the diagnostic method used and the geographic location. one hundred and fifty-two canine blood samples from six veterinary clinics and two shelters from sinaloa state (mexico) were analyzed in this study. all animals were suspected of having canine monocytic ehrlichiosis (cme). the diagnostic methods use ...201326464910
molecular epidemiologic survey of bartonella, ehrlichia, and anaplasma infections in japanese iriomote and tsushima leopard cats.the iriomote cat (ic; prionailurus iriomotensis) and the tsushima leopard cat (tlc; prionailurus bengalensis euptilura) are endangered wild felids in japan. as a part of ongoing conservation activities, we conducted a molecular, epidemiologic survey of bartonella, ehrlichia, and anaplasma infections in wild ic and tlc populations. blood samples (47 from 33 individual ic; 22 from 13 tlc) were collected between august 2002 and january 2011. using pcr analysis, we confirmed the presence of bartonel ...201323778615
a molecular survey of anaplasma spp., rickettsia spp., ehrlichia canis and babesia microti in foxes and fleas from sicily.fleas (insecta: siphonaptera) are obligate bloodsucking insects, which parasitize birds and mammals, and are distributed throughout the world. several species have been implicated in pathogen transmission. this study aimed to monitor red foxes and the fleas isolated from them in the palermo and ragusa provinces of sicily, italy, as these organisms are potential reservoirs and vectors of pathogens. thirteen foxes (vulpes vulpes) and 110 fleas were analysed by polymerase chain reaction (pcr) to de ...201324589112
[rhabdomyosarcoma in childhood. ii. ultrastructure].the diagnosis of rhabdomyosarcoma was established or proved by electron microscopy in two thirds of 21 cases. histological classification was not adequate or formulated as "undifferentiated sarcoma" in half the cases. electron microscopy did not succeed in one third of cases and rhabdomyosarcoma was classified according to simultaneous or successive study. undifferentiated tumour cells resembled myoblastema cells of the 5th-6th gestation weeks. differentiation of myoblasts and myotubes correspon ...20134064135
[rhabdomyosarcoma in childhood. ii. ultrastructure].the diagnosis of rhabdomyosarcoma was established or proved by electron microscopy in two thirds of 21 cases. histological classification was not adequate or formulated as "undifferentiated sarcoma" in half the cases. electron microscopy did not succeed in one third of cases and rhabdomyosarcoma was classified according to simultaneous or successive study. undifferentiated tumour cells resembled myoblastema cells of the 5th-6th gestation weeks. differentiation of myoblasts and myotubes correspon ...20134064135
intracranial hypertension in behçet's disease: demonstration of sinus occlusion with use of digital subtraction angiography.we describe two patients with behçet's disease who had symptomatic intracranial hypertension due to cerebral venous sinus thrombosis. the sinus thrombosis was demonstrated by digital subtraction angiography. in those patients with behçet's disease who have unexplained headaches, papilledema, and elevated cerebrospinal fluid pressure, venous digital subtraction angiography is an expedient, accurate, and safe procedure for demonstrating intracranial venous thrombosis.20133889512
[treatment of mushroom poisoning]. 20134590880
hospital design follows the crowd to ambulatory care. 20133804279
[how can the norwegian nurses' association use professional resources better?]. 20133649971
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