Publications

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hyperbaric respiratory mechanics. 20154949558
pattern and problems of surgical tuberculous disease in private surgical practice in nairobi. 20154459075
[effect of fermi resonance on the position and intensity of amide a and amide b bands in ir-spectra of polypeptides and proteins]. 20154425680
[taking care of the children of her homeland in denmark. nurse from bangladesh works in children's village in als]. 20154490231
[simultaneous acute arterial thromboembolism in the four limbs]. 20154425117
immunotherapy in treatment of breast cancer. 20154453580
[clinical features of psycho-organic development of traumatic genesis]. 20154450900
[comparative studies of microbiological purity of ointments by the direct culture method and use of membrane filters]. 20154447044
history of the community psychiatry movement. 20154894789
[presidential address. health is harmony]. 20154337670
[more advantages in education in special project form]. 20154499104
[preneoplastic processes in children]. 20154615674
[a new virus of the turnip yellow mosaic group: the gombo mosaic virus (hibiscus esculentus l. malvaceae)]. 20154345398
[in-vitro fusions of cultured somatic cells from drosophila melanogaster, induced with concanavalin a]. 20154631963
[general pathology of the fetus]. 20154645401
migraine--a personal view. 20154654756
industrial rehabilitation. 20154498643
studies of red-cell membrane function in heterozygous beta thalassaemia and other hypochromic anaemias. 20154441462
[clinical aspects and diagnosis of acquired esophagotracheal fistulas]. 20154452102
studies of gastrointestinal interactions. 20154615359
normal values for blood constituents of the baboon (papio species). 20154315779
anti-tumour necrosis factor treatment with adalimumab induces changes in the microbiota of crohn's disease.the composition of the intestinal microbiota is altered in crohn's disease [cd] patients. the objective of this study was to evaluate the qualitative and quantitative changes in the microbiota of cd patients in 3 months of treatment with adalimumab [ada], and determine whether or not these changes are produced towards the recovery of the normal, healthy-like microbiota.201526142465
identification of metabolic signatures linked to anti-inflammatory effects of faecalibacterium prausnitzii.faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified on the basis of human clinical data. the mechanisms underlying its beneficial effects are still unknown. gnotobiotic mice harboring f. prausnitzii (a2-165) and escherichia coli (k-12 jm105) were subjected to 2,4,6-trinitrobenzenesulfonic acid (tnbs)-induced acute colitis. the inflammatory colitis scores and a gas chromatography-time of flight (gc/tof) mass spectrometry-based metabolomic profile were monitored in ...201525900655
hydrodynamic chronoamperometry for probing kinetics of anaerobic microbial metabolism--case study of faecalibacterium prausnitzii.monitoring in vitro the metabolic activity of microorganisms aids bioprocesses and enables better understanding of microbial metabolism. redox mediators can be used for this purpose via different electrochemical techniques that are either complex or only provide non-continuous data. hydrodynamic chronoamperometry using a rotating disc electrode (rde) can alleviate these issues but was seldom used and is poorly characterized. the kinetics of faecalibacterium prausnitzii a2-165, a beneficial gut m ...201526127013
enhanced butyrate formation by cross-feeding between faecalibacterium prausnitzii and bifidobacterium adolescentis.cross-feeding is an important metabolic interaction mechanism of bacterial groups inhabiting the human colon and includes features such as the utilization of acetate by butyrate-producing bacteria as may occur between bifidobacterium and faecalibacterium genera. in this study, we assessed the utilization of different carbon sources (glucose, starch, inulin and fructooligosaccharides) by strains of both genera and selected the best suited combinations for evidencing this cross-feeding phenomenon. ...201526420851
manipulating the gut microbiota to maintain health and treat disease.the intestinal microbiota composition varies between healthy and diseased individuals for numerous diseases. although any cause or effect relationship between the alterations in the gut microbiota and disease is not always clear, targeting the intestinal microbiota might offer new possibilities for prevention and/or treatment of disease.201525651995
essential oils have different effects on human pathogenic and commensal bacteria in mixed faecal fermentations compared with pure cultures.a static batch culture system inoculated with human faeces was used to determine the influence of essential oil compounds (eocs) on mixed faecal microbiota. bacteria were quantified using quantitative pcr of 16s rrna genes. incubation for 24 h of diluted faeces from six individuals caused enrichment of bifidobacterium spp., but proportions of other major groups were unaffected. thymol and geraniol at 500 p.p.m. suppressed total bacteria, resulting in minimal fermentation. thymol at 100 p.p.m. ha ...201525500493
specific gut microbiota features and metabolic markers in postmenopausal women with obesity.gut microbial gene richness and specific bacterial species are associated with metabolic risk markers in humans, but the impact of host physiology and dietary habits on the link between the gut microbiota and metabolic markers remain unclear. the objective of this study was to identify gut metagenomic markers associated with estimates of insulin resistance, lipid metabolism and inflammation in obesity, and to explore whether the associations between metagenomic and metabolic markers persisted af ...201526075636
crohn associated microbial communities associated to colonic mucosal biopsies in patients of the western mediterranean.next generation sequencing approaches allow the retrieval of several orders of magnitude larger numbers of amplified single sequences in 16s rrna diversity surveys than classical methods. however, the sequences are only partial and thus lack sufficient resolution for a reliable identification. the opu approach used here, based on a tandem combination of high quality 454 sequences (mean >500 nuc) applying strict otu thresholds, and phylogenetic inference based on parsimony additions to preexistin ...201526275394
live faecalibacterium prausnitzii in an apical anaerobic model of the intestinal epithelial barrier.faecalibacterium prausnitzii, an abundant member of the human commensal microbiota, has been proposed to have a protective role in the intestine. however, it is an obligate anaerobe, difficult to co-culture in viable form with oxygen-requiring intestinal cells. to overcome this limitation, a unique apical anaerobic model of the intestinal barrier, which enabled co-culture of live obligate anaerobes with the human intestinal cell line caco-2, was developed. caco-2 cells remained viable and mainta ...201525224879
lactobacillus rhamnosus cncm i-3690 and the commensal bacterium faecalibacterium prausnitzii a2-165 exhibit similar protective effects to induced barrier hyper-permeability in mice.impaired gut barrier function has been reported in a wide range of diseases and syndromes and in some functional gastrointestinal disorders. in addition, there is increasing evidence that suggests the gut microbiota tightly regulates gut barrier function and recent studies demonstrate that probiotic bacteria can enhance barrier integrity. here, we aimed to investigate the effects of lactobacillus rhamnosus cncm i-3690 on intestinal barrier function. in vitro results using a caco-2 monolayer cell ...201525517879
recent advances in characterizing the gastrointestinal microbiome in crohn's disease: a systematic review.the intestinal microbiota is involved in the pathogenesis of inflammatory bowel disease. a reduction in the diversity of the intestinal microbiota as well as specific taxonomic and functional shifts have been reported in crohn's disease and may play a central role in the inflammatory process. the aim was to systematically review recent developments in the structural and functional changes observed in the gastrointestinal microbiome in patients with crohn's disease.201525844959
gut microbiota and tacrolimus dosing in kidney transplantation.tacrolimus dosing to establish therapeutic levels in recipients of organ transplants is a challenging task because of much interpatient and intrapatient variability in drug absorption, metabolism, and disposition. in view of the reported impact of gut microbial species on drug metabolism, we investigated the relationship between the gut microbiota and tacrolimus dosing requirements in this pilot study of adult kidney transplant recipients. serial fecal specimens were collected during the first m ...201525815766
monosodium l-glutamate and dietary fat differently modify the composition of the intestinal microbiota in growing pigs.the chinese have been undergone rapid transition to a high-fat diet-consuming lifestyle, while monosodium l-glutamate (msg) is widely used as a daily food additive. it has been reported that fat alters the composition of intestinal microbiota. however, little information is available on the effects of oral msg on intestinal microbiota, and no study was done focusing on the interaction effect of fat and msg with respect to intestinal microbiota. the present study thus aimed to determine the effec ...201525791341
increased gut microbiota diversity and abundance of faecalibacterium prausnitzii and akkermansia after fasting: a pilot study.an impaired gut microbiota has been reported as an important factor in the pathogenesis of obesity. weight reduction has already been mentioned to improve gut microbial subpopulations involved in inflammatory processes, though other subpopulations still need further investigation. thus, weight reduction in the context of a fasting program together with a probiotic intervention may improve the abundance and diversity of gut microbiota.201525763563
red wine consumption is associated with fecal microbiota and malondialdehyde in a human population.red wine intake has been associated with a lower risk of cardiovascular disease; its polyphenol content is the primary cause of antioxidant and anti-inflammatory properties attributed to this beverage. however, the way in which these activities are exerted is not yet clear, although some authors have proposed that intestinal microbiota could be implicated.201525738317
review on microbiota and effectiveness of probiotics use in older.the aim of the present systematic review is to summarize the existing knowledge about the human microbiota in the elderly and the effects of probiotics in elderly population. the elderly subjects, compared to adult population, show a reduction in the diversity of the microbiota, characterized by a large interindividual variability, with lower numbers of firmicutes, bifidobacteria, clostridium cluster xiv, faecalibacterium prausnitzii, blautia coccoides-eubacterium rectal and higher presence of e ...201525685762
high amount of dietary fiber not harmful but favorable for crohn disease.current chronic diseases are a reflection of the westernized diet that features a decreased consumption of dietary fiber. indigestible dietary fiber is metabolized by gut bacteria, including faecalibacterium prausnitzii, to butyrate, which has a critical role in colonic homeostasis owing to a variety of functions. dietary fiber intake has been significantly inversely associated with the risk of chronic diseases. crohn disease (cd) is not an exception. however, even authors who reported the inver ...201525663207
gut microbiota composition correlates with changes in body fat content due to weight loss.genetics, lifestyle, and dietary habits contribute to metabolic syndrome, but also an altered gut microbiota has been identified. based on this knowledge it is suggested that host bacterial composition tends to change in response to dietary factors and weight loss. the aim of this study was to identify bacteria affecting host metabolism in obesity during weight loss and to correlate them with changes of the body composition obtained from bioelectrical impedance analysis (bia). we recruited obese ...201525609655
the intestinal microbiome in spondyloarthritis.microbial dysbiosis in the gut is emerging as a common component in various inflammatory disorders including spondyloarthritis (spa). the depth of this influence has begun to be realized with next-generation sequencing of the gut microbiome providing unbiased assessment of previously uncharted bacterial populations.201526002022
faecalibacterium prausnitzii strain htf-f and its extracellular polymeric matrix attenuate clinical parameters in dss-induced colitis.a decrease in the abundance and biodiversity of intestinal bacteria within the firmicutes phylum has been associated with inflammatory bowel disease (ibd). in particular, the anti-inflammatory bacterium faecalibacterium prausnitzii, member of the firmicutes phylum and one of the most abundant species in healthy human colon, is underrepresented in the microbiota of ibd patients. the aim of this study was to investigate the immunomodulatory properties of f. prausnitzii strain a2-165, the biofilm f ...201525910186
faecalibacterium prausnitzii prevents physiological damages in a chronic low-grade inflammation murine model.the human gut houses one of the most complex and abundant ecosystems composed of up to 10(13)-10(14) microorganisms. the importance of this intestinal microbiota is highlighted when a disruption of the intestinal ecosystem equilibrium appears (a phenomenon called dysbiosis) leading to an illness status, such as inflammatory bowel diseases (ibd). indeed, the reduction of the commensal bacterium faecalibacterium prausnitzii (one of the most prevalent intestinal bacterial species in healthy adults) ...201525888448
microbiota-specific cd4cd8αα tregs: role in intestinal immune homeostasis and implications for ibd.in studies in murine models, active suppression by il-10-secreting foxp3 regulatory t cells (tregs) has emerged as an essential mechanism in colon homeostasis. however, the role of the equivalent subset in humans remains unclear, leading to suggestions that other subsets and/or mechanisms may substitute for foxp3 tregs in the maintenance of colon homeostasis. we recently described a new subset of cd4cd8αα t cells reactive to the gut bacterium faecalibacterium prausnitzii and endowed with regulat ...201526500657
role of the gut microbiome in obesity and diabetes mellitus.type 2 diabetes mellitus (t2dm) and obesity represent two of the biggest global health challenges of this century and are associated with significant comorbidities and healthcare costs. although multiple factors undoubtedly contribute to the development and progression of dm and obesity, research over the last decade has demonstrated that the microbes that colonize the human gut may play key contributory roles. gut microbes are now known to codevelop with the human host and are strongly influenc ...201526452391
equol status and changes in fecal microbiota in menopausal women receiving long-term treatment for menopause symptoms with a soy-isoflavone concentrate.the knowledge regarding the intestinal microbial types involved in isoflavone bioavailability and metabolism is still limited. the present work reports the influence of a treatment with isoflavones for 6 months on the fecal bacterial communities of 16 menopausal women, as determined by culturing and culture-independent microbial techniques. changes in fecal communities were analyzed with respect to the women's equol-producing phenotype. compared to baseline, at 1 and 3 months the counts for all ...201526300856
mucosa-associated faecalibacterium prausnitzii phylotype richness is reduced in patients with inflammatory bowel disease.faecalibacterium prausnitzii depletion in intestinal diseases has been extensively reported, but little is known about intraspecies variability. this work aims to determine if subjects with gastrointestinal disease host mucosa-associated f. prausnitzii populations different from those hosted by healthy individuals. a new species-specific pcr-denaturing gradient gel electrophoresis (pcr-dgge) method targeting the 16s rrna gene was developed to fingerprint f. prausnitzii populations in biopsy spec ...201526296733
(1)h nmr spectroscopy of fecal extracts enables detection of advanced colorectal neoplasia.colorectal cancer (crc) is a growing cause of mortality in developing countries, warranting investigation into its etiopathogenesis and earlier diagnosis. here, we investigated the fecal metabolic phenotype of patients with advanced colorectal neoplasia and controls using (1)h-nuclear magnetic resonance (nmr) spectroscopy and multivariate modeling. the fecal microbiota composition was assessed by quantitative real-time pcr as well as wif-1 methylation levels in stools, serum, and urine and corre ...201526211820
structural analysis of a class iii preq1 riboswitch reveals an aptamer distant from a ribosome-binding site regulated by fast dynamics.preq1-iii riboswitches are newly identified rna elements that control bacterial genes in response to preq1 (7-aminomethyl-7-deazaguanine), a precursor to the essential hypermodified trna base queuosine. although numerous riboswitches fold as h-type or hlout-type pseudoknots that integrate ligand-binding and regulatory sequences within a single folded domain, the preq1-iii riboswitch aptamer forms a hlout-type pseudoknot that does not appear to incorporate its ribosome-binding site (rbs). to unde ...201526106162
randomised clinical trial: gut microbiome biomarkers are associated with clinical response to a low fodmap diet in children with the irritable bowel syndrome.a low fermentable oligosaccharides, disaccharides, monosaccharides and polyols (fodmap) diet can ameliorate symptoms in adult irritable bowel syndrome (ibs) within 48 h.201526104013
oral administration of faecalibacterium prausnitzii decreased the incidence of severe diarrhea and related mortality rate and increased weight gain in preweaned dairy heifers.probiotics are a promising alternative to improve food animal productivity and health. however, scientific evidence that specific microbes can be used to benefit animal health and performance is limited. the objective of this study was to evaluate the effects of administering a live culture of faecalibacterium prausnitzii to newborn dairy calves on subsequent growth, health, and fecal microbiome. initially, a safety trial was conducted using 30 newborn bull calves to assess potential adverse eff ...201526710101
potential association of vacuum cleaning frequency with an altered gut microbiota in pregnant women and their 2-year-old children.westernized lifestyle and hygienic behavior have contributed to dramatic changes in the human-associated microbiota. this particularly relates to indoor activities such as house cleaning. we therefore investigated the associations between washing and vacuum cleaning frequency and the gut microbiota composition in a large longitudinal cohort of mothers and their children. the gut microbiota composition was determined using 16s ribosomal rna (rrna) gene illumina deep sequencing.201526687338
modulation of the gut microbiota composition by rifaximin in non-constipated irritable bowel syndrome patients: a molecular approach.rifaximin, with its low systemic absorption, may represent a treatment of choice for irritable bowel syndrome (ibs), mainly due to its ability to act on ibs pathogenesis, through the influence on gut microbiota. the aim of the present study was to assess, by biomolecular tools, the rifaximin active modulation exerted on gut microbiota of non-constipated ibs patients. fifteen non-constipated ibs subjects were treated with 550 mg rifaximin three times a day for 14 days. stool samples were collecte ...201526673000
[effects of faecalibacterium prausnitzii supernatant on th17 cell and il-17a in dextran sulfate sodium-induced ulcerative colitis mice].to explore the protective and therapeutic effects of faecalibacterium prausnitzii (fp) supernatant on ulcerative colitis (uc) in mice induced by dextran sulfate sodium (dss) and the underlying mechanisms.201526739073
a simple coculture system shows mutualism between anaerobic faecalibacteria and epithelial caco-2 cells.most gut bacteria are obligate anaerobes and are important for human health. however, little mechanistic insight is available on the health benefits of specific anaerobic gut bacteria. a main obstacle in generating such knowledge is the lack of simple and robust coculturing methods for anaerobic bacteria and oxygen-requiring human cells. here, we describe the development of a coculture system for intestinal caco-2 cells and an anaerobic symbiont, faecalibacterium prausnitzii, making use of 50 ml ...201526667159
in vitro fermentation of lupin seeds (lupinus albus) and broad beans (vicia faba): dynamic modulation of the intestinal microbiota and metabolomic output.broad beans (vicia faba) and lupin seeds (lupinus albus) are legumes rich in a wide range of compounds, which may represent a useful dietary approach for modulating the human gut microbiome. in this work, after in vitro digestion, legume samples were used as carbon sources in anaerobic batch cultures to evaluate their impact on the intestinal microbiota composition and on their metabolic products. the fermentations were monitored by a decrease in ph, generation of short chain fatty acids (scfa) ...201526252418
probiotics: a proactive approach to health. a symposium report.this report summarises talks given at the 8th international yakult symposium, held on 23-24 april 2015 in berlin. two presentations explored different aspects of probiotic intervention: the small intestine as a probiotic target and inclusion of probiotics into integrative approaches to gastroenterology. probiotic recommendations in gastroenterology guidelines and current data on probiotic efficacy in paediatric patients were reviewed. updates were given on probiotic and gut microbiota research i ...201526548336
renaissance of the retinal pigment epithelium. 20155049449
[fluorescence angiography of the retina in the diagnosis of diabetic retinopathy]. 20154667893
the compatibility of anxiety and actualization. 20154418409
base composition of dna isolated from fresh water sponge, ephydatia meyeni. 20154644880
bacillus coagulans gbi-30, 6086 modulates faecalibacterium prausnitzii in older men and women.advancing age is linked to a decrease in beneficial bacteria such as bifidobacterium spp. and reduced aspects of innate immune function.201525948780
[skull changes in ostitis deformans (paget). an important comment on a publication by j. haym in zwr 82 (1973) p. 325-327]. 20154522135
[surgery of complete endocardial cushion defect (rastelli type c)]. 20154477173
[fusional reserves in children of various ages depending on the nature of the refraction]. 20154409634
the estrogen-creatinine ratio and the "estrogen index" in normal pregnancy. 20154637034
the self-help phenomenon. 20154489849
movement and reproduction of cells during formation of somites in chick embryos. 20154658080
[progress in pharmaceutical technology during 1970 and 1971]. 20154575871
reaction of 24,28-epoxides of sterol side chain with boron trifluoride etherate. 20154709672
crushing chest injuries. 20154702862
crushing chest injuries. 20154702862
[bundle-branch block in acute myocardial infarct. a review of 48 cases]. 20154641912
healthcaremanship. 20154669991
case report. intussusception of the base of the appendix. 20154669990
[measures for improved function and reconstruction. mucogingival surgery]. 20154500982
[proceedings: acute myocardial infarct associated with cerebral vascular diseases]. 20154477109
a survey of selected vaginal flora in malawian women. 20154755303
feeding of wildcat in azerbaijan. 20154668790
primary, early bone grafting in complete grafts of the lip and palate. a follow-up study of 53 cases. 20154615372
[class v porcelain inlays (author's transl)]. 20154524141
management of marital problems. 20154378584
relative stability of hetacillin and ampicillin in solution. 20154339954
the composition of chinchilla milk. 20154685603
diet and coronary heart disease. a council statement. 20154678368
neoplasms of the urinary tract. 20154675645
[changes of the finger plethysmogram during and after heart surgery--its application as a bedside monitor]. 20154672356
[extra-african forms of burkitt's tumor (description of a case of malignant lymphoma of the tibia)]. 20154654184
[disorder of external respiration in pulmonary tuberculosis]. 20154659462
[importance of the association of cloxacillin and ampicillin in orl]. 20154697132
clinical trial of an acridane derivative (skf no. 14,336) in male schizophrenics. 20154963018
effects of atmospheric and extra-terrestrial electromagnetic and corpuscular radiations on living organisms. 20154621279
in memoriam p.e. lukomsky 1900-1974. 20154615878
[secondary microflora in tuberculosis of the urogenital tract]. 20154605937
selection of the best method in stereology. 20154599320
gastric response to metiamide.metiamide greatly reduced pentagastrin-stimulated and overnight secretion of acid and pepsin in 11 patients with duodenal ulcer and virtually abolished gastric secretion in three patients with gastric ulcer. the drug was equally effective when infused intravenously or intraduodenally. a therapeutic trial of metiamide is warranted in diseases caused or aggravated by excess gastric secretion of acid and pepsin.20154597122
airway closure in children. 20154643846
the diagnosis and prevention of post operative venous thrombosis. 20154617329
[etiological criteria in infantile cerebral palsy]. 20154744122
[etiological criteria in infantile cerebral palsy]. 20154744122
[adhesion and retention of various alginate impression compounds to the tray]. 20154569432
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