Publications

detection and quantitation of bacteroides gingivalis in bacterial mixtures by means of flow cytometry.		1984	6084706
characterization of a trypsin-like protease from the bacterium bacteroides gingivalis isolated from human dental plaque.	a trypsin-like, membrane-bound protease from bacteroides gingivalis was solubilized by triton x-100 and partially purified by a combination of deae-sepharose and aminophenylmercuric sepharose chromatography, by taking advantage of the thiol group on the enzyme. the purified enzyme hydrolysed the synthetic substrates benzoyl-l-arginine-p-nitroanilide (l-bapa), benzoyl-d,l-arginine-beta-naphthylamide (bana) and tosyl-l-arginine methyl ester, as well as bovine serum albumin and ovalbumin, but not t ...	1984	6089721
immunogold electron microscopy of surface antigens of oral bacteria.	colloidal gold particles 3-6 nm in diameter were prepared and stabilized with the igg fraction of polyspecific rabbit antisera produced against four different oral bacteria. the immunogold markers were used in homologous reactions to label the bacteria in a preembedding procedure for electron microscopy. an indirect immunofluorescence procedure was concurrently used to optimize the labelling conditions before observation with the electron microscope. the immunogold markers labelled fibrillar str ...	1984	6093974
igg, iga and igm serum antibodies against lipopolysaccharide from bacteroides gingivalis in periodontal health and disease.		1985	2933501
hepatitis b vaccine use in chronic hemodialysis centers in the united states.	the centers for disease control surveyed 1,255 chronic hemodialysis centers in the united states in 1983 to assess the use of hepatitis b vaccine as well as the risk of acquiring hepatitis b among both patients and staff. the response rate to a mailed questionnaire was 90%. seventy-one percent of the centers reported that they gave vaccine, but only an estimated 6% of susceptible patients and 32% of susceptible staff received all three doses of vaccine. centers more likely to give vaccine includ ...	1985	2933534
thyroxine induced transformation in sarcoplasmic reticulum of rabbit soleus and psoas muscles.	the properties of the sarcoplasmic reticulum membranes isolated from slow-twitch type i soleus and fast-twitch type ii psoas muscles of control and thyroxine treated rabbits were comparatively studied. membrane yield, maximal calcium storing capacity, atp-supported calcium uptake, calcium-dependent atpase activity and calcium-dependent phosphoprotein formation were found to be 3-10 fold higher in psoas than in soleus preparations. membrane yield, calcium-dependent atpase activity, atp-supported ...	1985	2934902
detection of actinobacillus actinomycetemcomitans and bacteroides gingivalis in subgingival smears by the indirect fluorescent-antibody technique.		1985	2935616
restriction nuclease digestions driven to completion by escherichia coli rna polymerase and t4 gene 32 protein.	restriction enzyme digestions of large scale dna preparations often do not go to completion. this is due to product inhibition by the newly generated ends of the digested dna. the addition of exogenous proteins that bind tightly to the free ends of dna or to single-stranded dna will relieve this inhibition. we show that a considerable savings on restriction nucleases can be attained by the addition of rna polymerase or t4 gene 32 protein in stoichiometric amounts to the newly produced dna ends.	1985	2981211
influence of drugs that block calcium channels on the microbicidal function of human neutrophils.	the central role of calcium ions in cell physiology prompted us to examine the hypothesis that pharmacological concentrations of calcium channel-blocking drugs might affect human neutrophil (pmn) functions. the capacity of pmns suspended in verapamil hydrochloride for killing pseudomonas aeruginosa during two hour incubations was significantly impaired (p less than .05). several observations suggested that this drug effect was the result of altered calcium metabolism: exposure to verapamil decre ...	1985	2981275
characterization of a major protein with a molecular weight of 160,000 associated with the viral capsid of epstein-barr virus.	a monoclonal antibody designated v3 was produced against a late protein associated with the epstein-barr virus-induced viral capsid antigen complex. the antibody reacted with discrete patches in the nuclei of infected cells as well as with virus particles, as shown by immunofluorescence and ultrastructural immunoperoxidase staining. the molecular weight of the protein precipitated by this monoclonal antibody was ca. 160,000. all anti-viral capsid antigen antibody-positive sera tested in an enzym ...	1985	2981328
characterization of an rna polymerase activity from hela cell mitochondria, which initiates transcription at the heavy strand rrna promoter and the light strand promoter in human mitochondrial dna.	an rna polymerase activity capable of initiating transcription at both the heavy strand rrna promoter and the light strand promoter of human mitochondrial dna has been partially purified from hela cell mitochondria and characterized in its requirements and products. the ratio of the two transcription initiating activities varied considerably from preparation to preparation. the human mtrna polymerase partially purified by deae-cellulose and heparin-agarose chromatography exhibits a great sensiti ...	1985	2981880
free radicals and myocardial ischemia. the role of xanthine oxidase.	recent studies have established a major role for oxygen-derived free radicals in post ischemic tissue injury to the intestine. during ischemia, there appears to be a calcium-triggered, protease-dependent conversion of the native xanthine dehydrogenase to a superoxide-producing xanthine oxidase. the catabolic degradation of atp during ischemia provides an oxidizable substrate, hypoxanthine. on reperfusion, molecular oxygen is resupplied and a burst of superoxide production ensues, resulting in ex ...	1985	2982206
detection of collagenase activity in oral bacteria.	collagenolytic activity of 12 species of oral bacteria was assessed using two methods of detection. except for two species, all bacterial strains tested were capable of degrading at least one general protein substrate. results of collagenolytic activity in a growth assay indicate that bacteroides gingivalis is the only bacterium capable of degrading collagen when the substrate is sterilized using ethylene oxide. however, if the substrate is sterilized by autoclaving, in the presence or absence o ...	1985	2985228
[computed tomography of the heart. technic for quantitative evaluation of the perfusion of the ischemic myocardial region. experimental basis].	a ct standard procedure has been developed in six series of experiments, using 62 anaesthetised dogs in order to quantitative relative blood flow through ischaemic heart muscle. measurements of cardiac dynamics by means of contrast media were less strictly controlled. the use of the standardised procedures with contrast injections is necessary because of the variable perfusion and washout intervals of the contrast in normal and ischaemic myocardium.	1985	2988058
rapid purification of human placental angiotensin i converting enzyme by captopril affinity chromatography.		1985	2989307
estradiol increases choline acetyltransferase activity in specific basal forebrain nuclei and projection areas of female rats.	administration of estradiol to gonadectomized female, but not male rats, is associated with increased activity of choline acetyltransferase in the medial aspect of the horizontal diagonal band nucleus, the frontal cortex, and ca1 of the dorsal hippocampus. four other basal forebrain cholinergic nuclei did not show changes in choline acetyltransferase activity after estradiol. these data have implications for possible benefits of estradiol administration to patients with senile dementia of the al ...	1985	2990988
construction and biological analysis of deletion mutants of fujinami sarcoma virus: 5'-fps sequence has a role in the transforming activity.	fujinami sarcoma virus (fsv) genome codes for the gag-fps fusion protein fsv-p130. the amino acid sequence of the 3' one-third portion in v-fps is partially homologous to the 3' half of pp60src, or the kinase domain, but the sequence of the 5' portion is unique to v-fps. to identify a possible domain structure in the v-fps sequence responsible for cell transformation, we constructed various deletion mutants of fsv with molecularly cloned viral dna. their transforming activities were assayed by m ...	1985	2991588
[the vaccine for hepatitis b virus. definition of criteria for its use].		1985	2994199
quantitative localization of [3h]tcp binding in rat brain by light microscopy autoradiography.	the anatomical localization of phencyclidine (pcp)/sigma-opiate receptors in rat brain was determined by quantitative light microscopy autoradiography using the new ligand n-(1-[2-thienyl]cyclohexyl) [3h]piperidine ([3h]tcp). tcp is a potent analog of pcp which possesses a higher affinity for pcp/sigma-opiate receptor than does pcp itself. the highest level of [3h]tcp binding was detected in the hippocampus. intermediate levels were found in frontal cortex, striatum, amygdala and cerebellum. spe ...	1985	2994834
a powerful method for the preparation of cdna libraries: isolation of cdna encoding a 100-kdal nucleolar protein.	an easy and quick method to synthesize large cdna molecules and to clone them with very high efficiency in the expression vector lambda gt11 is described. the technique employs rnase h and escherichia coli dna ligase treatment during second-strand synthesis, followed by repair of the ds cdna extremities by s1 nuclease and polik (klenow fragment) treatment. this treatment allows efficient addition of suitable linkers and results in a 100-fold increase in the yield of cloned cdna, when compared wi ...	1985	2996985
autoxidation reactions of hemoglobin a free from other red cell components: a minimal mechanism.	rates of autoxidation reactions are determined for normal human hemoglobin a preparations which are extensively purified to remove all other redox active red cell components. the effects of superoxide dismutase, catalase, and hydroxyl radical scavengers on the reaction provide evidence for superoxide formation as the rate determing step followed by fast reactions that involve peroxide and hydroxyl radical. these results support a minimum overall mechanism for heme iron(ii) oxidation and dioxygen ...	1985	2998382
a long and complex enhancer activates transcription of the gene coding for the highly abundant immediate early mrna in murine cytomegalovirus.	using the simian virus 40 "enhancer trap" approach, we have identified a transcription enhancer located just upstream of the major immediate early gene of murine cytomegalovirus. this enhancer has several striking properties. (i) together with the enhancer of human cytomegalovirus, it is the strongest transcription enhancer found to date. (ii) it is an extremely long enhancer, spanning greater than 700 base pairs. (iii) it consists of a rather complex pattern of sequence repeats, the longest of ...	1985	3001696
immunization against dental caries.	prevention of dental caries has been investigated by immunization with streptococcus mutans in rodents and subhuman primates. in addition to cells and cell walls of s. mutans, the enzyme glucosyl transferase and purified protein antigens prepared from s. mutans were successfully used in immunization against dental caries. subcutaneous immunization of rhesus monkeys elicits significant levels of serum igg, igm and iga antibodies, lymphorproliferative response and t cell helper activity to s. muta ...	1985	3159164
characterization of neurospora crassa cyclic amp phosphodiesterase activated by calmodulin.	activation of cyclic amp phosphodiesterase i by brain or neurospora calmodulin was studied. the stimulation required micromolar concentrations of ca2+, and it was observed at cyclic amp concentrations between 0.1 and 500 microm. activation was blocked by edta and some neuroleptic drugs such as chlorpromazine and fluphenazine. these drugs inhibit the elongation of n. crassa wild-type aerial hyphae. these results reinforce the evidence towards the recognition of ca2+-calmodulin as one of the syste ...	1985	3004404
[specific protein in the plasma of rheumatoid arthritis patients].	a unique protein was detected in the plasma of rheumatoid arthritis patients by two-dimensional electrophoresis. we named it rheumatoid arthritis specific protein (rasp). according to the pattern and concentration of its spot, ra patients were classified into three groups as rasp+, rasp +/- and rasp-. rasp+ was detected only in ra and its occurrence was 45/120 (38%). the occurrence of rasp including rasp +/- was 84/120 (70%) in ra, 6/13 (46%) in sle, 2/8 (25%) in pss, 11/51 (22%) in liver diseas ...	1985	3831162
biological characterization of human bone tumors. vii. detection of malignancy in a giant cell tumor of bone by flow cytometric dna-analysis.	the biological behavior of giant cell tumors of bone often cannot be definitively evaluated by light and electron microscopical criteria. as dna aneuploidy was found to be a highly sensitive marker of malignant cells, we have analyzed this descriptive parameter in 4 giant cell tumors of bone by flow cytometry. three of the four cases were non-metastasizing giant cell tumors, the fourth was a clinically malignant neoplasm presenting extensive visceral metastases four years after first diagnosis. ...	1985	3831997
effect on wool growth of thyroxine replacement in thyroidectomized merino rams.	the effect on wool growth of thyroidectomy with subsequent thyroxine replacement at subnormal and supranormal levels has been investigated in merino rams fed a restricted basal diet. thyroidectomy without thyroxine replacement caused a greater than 60% reduction in wool growth. provision of 30% of normal plasma thyroxine concentrations was sufficient to return wool growth to normal. similarly, complete replacement of plasma thyroxine levels gave normal wool growth while increasing thyroxine conc ...	1985	3834890
[pathogenic staphylococci and salmonellae in commercial yogurt].		1985	3836492
inheritance of the human salivary proline-rich proteins: a reinterpretation in terms of six loci forming two subfamilies.	dna studies suggest that six loci control the synthesis of human salivary proline-rich proteins (prps). genes at two of these loci (proposed names, prh1 and prh2) contain regions that strongly hybridize to a probe made from a cdna in which sites for the restriction enzyme haeiii occur repeatedly; they code for the acidic prps. genes at the remaining four loci (prb1, prb2, prb3, and prb4) contain regions that strongly hybridize to a probe with repeated bstn1 sites; they probably code for the basi ...	1985	3840016
regional distribution and release of peptide yy with fatty acids of different chain length.	the present study examines peptide yy responses to regional intestinal perfusion of fatty acids of different chain length--dodecanoate and oleate. six dogs with chronic gastric, duodenal, and jejunal fistulas were studied. proximal perfusates were administered into the duodenum and diverted through an intestinal fistula placed 45 cm beyond the duodenal cannula. distal perfusates were administered into the caudal stoma of this intestinal stoma. peptide yy responses to proximal, distal, and whole- ...	1985	3841263
changes in the sedimentation properties of cytosolic androgen receptors associated with activation in vitro and in vivo.	in cell-free systems androgen receptor (ar) labeled with (3h)dht at 0 degrees c in the presence of 50mm molybdate remains unactivated (less than 3% binding to nuclei) and untransformed (7-8s on sucrose density gradients containing 0.4m kcl and 50mm molybdate). in the absence of molybdate, however, these complexes undergo activation and transformation even at 0 degrees c, albeit, very slowly. incubation of unactivated, untransformed ar complexes at 18 degrees c, or at 0 degrees c in the presence ...	1985	3842022
localization of anti-osteogenic sarcoma monoclonal antibody 791t/36 in a primary human osteogenic sarcoma and its subsequent xenograft in immunodeprived mice.	a primary human osteogenic sarcoma was visualized in situ by external gamma-camera imaging following administration of 131i-labelled anti-osteogenic sarcoma monoclonal antibody 791t/36. a xenograft of the tumour established in immunodeprived mice also showed localization of 131i-791t/36 determined by both gamma-camera imaging and organ distribution studies. radiolabelled normal immunoglobulin showed no tumour localization. expression of the 791t/36-defined antigen on xenografted tissue was furth ...	1985	3856475
selective uptake of a toxic lipophilic anthracycline derivative by the low-density lipoprotein receptor pathway in cultured fibroblasts.	n-(n-retinoyl)-l-leucyldoxorubicin 14-linoleate (r11-dox), a new lipophilic derivative of doxorubicin, was synthesized and incorporated into low-density lipoprotein (ldl). the drug-ldl complex contained 100-200 drug molecules/ldl particle. when cultured normal human fibroblasts were incubated with 125i-ldl-incorporated drug, there was a perfect correlation between the cellular uptake plus degradation of 125i-ldl and the cellular drug accumulation. the presence of excess native ldl inhibited the ...	1985	3856679
[estradiol hormone receptors in human gingiva: preliminary results].		1985	3858256
inactivation of guinea-pig serum proteinase inhibitors by bacteroides gingivalis.	the activity of proteinase inhibitors of guinea-pig serum was evaluated by using trypsin as test enzyme. strains of black-pigmented bacteroides species were incubated with serum under anaerobic conditions. strains of bacteroides gingivalis inactivated the trypsin inhibitors of guinea-pig serum within 1 h, while the other strains reduced the activity of the trypsin inhibitors by less than 50% within 48 h. the inactivation of proteinase inhibitors of guinea-pig serum by b. gingivalis may be an imp ...	1985	3858964
animal behavioral models in the discovery of compounds to treat memory dysfunction.		1985	3860092
relationship between some subgingival bacteria and periodontal pocket depth and gain or loss of periodontal attachment after treatment of adult periodontitis.	we studied the association between post-treatment periodontal disease activity and subgingival bacteroides gingivalis, bacteroides intermedius, spirochetes and motile rods. 20 adults, 22-62 years, with moderate-to-severe periodontitis participated in a split-mouth treatment study. all individual quadrants received supragingival cleaning and in addition, subgingival scaling and a nahco3-nacl-h2o2 slurry, subgingival scaling alone, slurry alone, or no subgingival treatment. post-treatment periodon ...	1985	3860517
[protective efficacy of antiserum against bacteroides gingivalis].		1985	3861726
[depression of neutrophil chemotaxis and lysosomal enzyme release by bacteroides gingivalis].		1985	3861740
molecular cloning and nucleotide sequence of human glucocerebrosidase cdna.	mutations in the human glucocerebrosidase gene cause gaucher disease. a cdna clone containing the entire human glucocerebrosidase coding region from normal cells has been isolated using lambda gt11 expression libraries. the complete nucleotide sequence, a restriction map, and a hydropathy profile are presented. hybridization to chromosome-specific dna localizes the human glucocerebrosidase gene to chromosome 1. the likely precursor protein is 515 amino acids long. the nh2-terminal 19 amino acids ...	1985	3864160
invasive pulmonary aspergillosis in acute leukemia: characteristic findings on ct, the ct halo sign, and the role of ct in early diagnosis.	invasive pulmonary aspergillosis (ipa) in immunocompromised patients is often difficult to diagnose. many pathogens present initially with similar, nonspecific pulmonary findings. air crescent formation has been reported to be highly suggestive of ipa in the appropriate clinical setting, but this is a late sign in an otherwise rapidly fatal infection. the authors reviewed the available chest computed tomography (ct) scans of nine patients with acute leukemia and documented ipa, including four pa ...	1985	3864189
biosynthesis of glycosphingolipids by human myeloid leukemia cells.	we have performed comparative studies of the neutral glycosphingolipids synthesized by three human myeloid leukemia cell lines, k562, kg1, and hl-60, which were metabolically labeled with [14c]galactose, to evaluate changes in neutral glycosphingolipid synthesis with myeloid cell differentiation. individual neutral glycosphingolipids containing one to four sugars were purified by a combination of the following methods: diethylaminoethyl-sephadex column chromatography, acetylation-florisil column ...	1985	3865690
[monoclonal antibodies against surface antigens of bacteroides gingivalis].		1985	3867153
surface components involved in bacterial pathogen-plant host recognition.	during their initial association with plant hosts, pathogenic bacteria interact with plant cell walls. the results of this interaction appear to determine whether bacterial multiplication will take place. with one group of bacterial plant pathogens (e.g. agrobacterium tumefaciens), attachment to the host surface appears essential for pathogenesis. with another group (e.g. pseudomonas solanacearum), only those strains that do not attach to the host cell wall are able to multiply in the intercellu ...	1985	3867676
the relationships between streptococcal species and periodontopathic bacteria in human dental plaque.	the existence of antagonistic and commensal relationships between microorganisms was investigated. the predominant cultivable flora in 172 plaque samples from active and non-active sites in 32 human subjects with destructive periodontitis was determined. the presence of putative periodontopathic organisms (bacteroides gingivalis, bacteroides intermedius, bacteroides forsythus, wolinella recta, actinobacillus actinomycetemcomitans, and eikenella corrodens) in a site was correlated with the absenc ...	1985	3868968
[inhibitory effect of bacteroides gingivalis on fibroblast proliferation and stimulated lymphocytes].		1985	3869049
[use of bacteroides gingivalis-specific monoclonal antibodies for immunodiagnosis of adult periodontal disease].		1985	3869060
[partial purification and characterization of exohemagglutinin from bacteroides gingivalis 381].		1985	3869171
bacterial profiles of subgingival plaques in periodontitis.	in this report over 400 subgingival plaque samples taken from over 110 patients were examined microscopically and culturally for 30 bacterial parameters. the patients could be placed into six disease categories based upon clinical criteria. the bacterial profile of each clinical category was generally distinctive of that category. periodontal patients who had been successfully treated and maintained had plaques that were populated by significantly higher proportions of streptococcus sanguis, act ...	1985	3869648
generalized juvenile periodontitis, defective neutrophil chemotaxis and bacteroides gingivalis in a 13-year-old female. a case report.	host immune responses and the predominant subgingival microflora were evaluated in a 13-year-old female exhibiting a severe form of generalized juvenile periodontitis. the patient's neutrophils were chemotactically depressed but exhibited a normal oxidative capacity. serum igg antibody to bacteroides gingivalis and to actinobacillus actinomycetemcomitans serotype c were elevated. significantly, b. gingivalis constituted 8 to 16% of the cultivable microflora and 13 to 20% of the total cell count ...	1985	3869649
reaction of human sera from juvenile periodontitis, rapidly progressive periodontitis, and adult periodontitis patients with selected periodontopathogens.	the levels of serum antibody reactive to selected periodontopathogens were determined in 182 clinically characterized patients: 35 healthy control, 50 juvenile periodontitis, 42 adult periodontitis and 55 rapidly progressive periodontitis. reactive antibody levels were determined using an enzyme-linked immunosorbent assay with whole cell preparations of bacteroides gingivalis, capnocytophaga (bacteroides) ochraceus, fusobacterium nucleatum and actinobacillus actinomycetemcomitans (y-4) serving a ...	1985	3869650
epiglottitis.		1985	3871691
a permeability barrier as a mechanism of chloramphenicol resistance in haemophilus influenzae.	chloramphenicol resistance in haemophilus influenzae occurs most frequently via plasmid-mediated chloramphenicol acetyltransferase production. we studied four strains with high-level chloramphenicol resistance (mic greater than 20 micrograms/ml) which did not have detectable chloramphenicol acetyltransferase activity. the chloramphenicol resistance determinant was transformed into a chloramphenicol-susceptible laboratory h. influenzae strain from each of the four wild-type strains, enabling isog ...	1985	3872625
human b-lymphocyte colony responses: suboptimal colony responsiveness in aged humans associated with defective function of b cells and monocytes.	the abilities of human b cells from young and aged subjects to form colonies in semisolid cultures stimulated with staphylococcus protein a were investigated. approximately three-fourths of aged adults had significantly diminished colony responses compared to young adults. in 55% of these aged adults, the in vitro blocking of monocyte prostaglandin synthesis lead to a 1.5-fold or greater augmentation of the depressed colony responses. other experiments showed that the improvement with indomethac ...	1985	3874702
human b-lymphocyte colony responses: suboptimal colony responsiveness in aged humans associated with defective function of b cells and monocytes.	the abilities of human b cells from young and aged subjects to form colonies in semisolid cultures stimulated with staphylococcus protein a were investigated. approximately three-fourths of aged adults had significantly diminished colony responses compared to young adults. in 55% of these aged adults, the in vitro blocking of monocyte prostaglandin synthesis lead to a 1.5-fold or greater augmentation of the depressed colony responses. other experiments showed that the improvement with indomethac ...	1985	3874702
polyclonal b cell activation during the course of angiostrongylus cantonensis infection.	the numbers of igm antibody forming cells against various heterologous antigens: srbc, hrbc, tnp and fitc, were found to be elevated in the spleens of rats after infection with angiostrongylus cantonensis. in these rats, the number of splenic b cells and total igg and igm levels in serum also increased spontaneously about 2- to 3-fold of uninfected rats during the course of the infection. these results suggested that a. cantonensis infection may activate b cells polyclonally in the spleens of th ...	1985	3876249
functional role of interleukin 1 in periodontal disease: induction of interleukin 1 production by bacteroides gingivalis lipopolysaccharide in peritoneal macrophages from c3h/hen and c3h/hej mice.	hot phenol-water-extracted lipopolysaccharide (lps) from bacteroides gingivalis 381 was purified by sephadex g-100 chromatography with tris buffer supplemented with sodium deoxycholate and edta (b-lps). in the present study, b-lps was examined for its ability to induce interleukin 1 (il-1) production, a mitogenic response, and macrophage activation in lps high-responder c3h/hen and low-responder c3h/hej mice. a significant increase in il-1 production was observed in c3h/hen and c3h/hej peritonea ...	1985	3876285
early rheumatoid-like synovial lesions in rabbits drinking cow's milk. i. joint pathology.	rheumatoid-like lesions developed in 9 of 25 (36%) old english rabbits drinking cow's milk for 12 weeks. the incidence of lesions in male and female animals was similar. the majority of rabbits drinking cow's milk developed increased numbers of nucleated cells and raised percentages of t lymphocytes in their synovial fluids, compared to control rabbits. the cell counts and t cell percentages correlated with the severity of the histological lesions. no evidence of glomerulonephritis was observed ...	1985	3876289
structure of lipopolysaccharide from bacteroides gingivalis.		1985	3880031
effects of calcium-channel blockers on myocardial preservation during experimental acute myocardial infarction.	calcium antagonists have become accepted agents for the attenuation of myocardial ischemia when it becomes manifest as angina pectoris. however, it is not known whether these agents can protect ischemic myocardium during the early evolution of an acute myocardial infarct. calcium antagonists could potentially improve myocardial perfusion, by relieving coronary spasm or improving collateral blood flow, and reduce the energy demands of the ischemic myocardium either directly or by reducing heart r ...	1985	3881903
biochemical and immunobiological properties of lipopolysaccharide (lps) from bacteroides gingivalis and comparison with lps from escherichia coli.	lipopolysaccharides (lpss) were isolated from bacteroides gingivalis and escherichia coli by the phenol-water and butanol-water procedures. the phenol-water-extracted lps from b. gingivalis 381 was composed of 46% carbohydrate, 23% hexosamine, 18% fatty acid, and 5% protein. the major component sugars of this preparation were glucose, glucosamine, rhamnose, galactose, galactosamine, and mannose, and their molecular ratio was 1:0.9:0.7:0.6:0.6:0.4, respectively. neither heptose nor 2-keto-3-deoxy ...	1985	3882561
glycylprolyl dipeptidylaminopeptidase from bacteroides gingivalis.	dipeptidyl aminopeptidase activity was found in the culture medium of bacteroides gingivalis 381. the enzyme, hydrolyzing glycylprolyl-4-methylcoumaryl-7-amide, was purified 750-fold from culture medium by ammonium sulfate precipitation, sephadex g-200 gel filtration, and deae bio gel a column chromatography. the molecular weight, determined by gel filtration, was approximately 160,000. the isoelectric point of the enzyme, estimated by isoelectric focusing using polyacrylamide disk gel electroph ...	1985	3882793
a newly recognized cause of travelers' diarrhea: enteroadherent escherichia coli.	adherence to hep-2 tissue culture cells has been proposed as a virulence characteristic of enteropathogenic escherichia coli (epec). a preliminary study revealed that e. coli that adhered to hep-2 cells, but did not produce conventional enterotoxins and did not belong to recognized epec serogroups, could be isolated from adults from the united states who acquired diarrhea in mexico. the purpose of this study was to determine the prevalence of these enteroadherent e. coli (eaec) in 188 travelers ...	1985	3882849
[a simple culture method for the bacteriological identification of bacillus larvae on columbia blood-slant agar].		1985	3883984
[a simple culture method for the bacteriological identification of bacillus larvae on columbia blood-slant agar].		1985	3883984
the opioid octapeptide met5-enkephalin-arg6-gly7-leu8: characterization and distribution in rat spinal cord.	the regional quantitation, immunohistochemical localization and molecular heterogeneity of met5-enkephalin-arg6-gly7-leu8 were examined in rat spinal cord with a specific radioimmunoassay. a rostrocaudal gradient in met5-enkephalin-arg6-gly7-leu8 content was observed; the highest levels occurred in sacral cord. dorsal cord content was higher than that of ventral cord at all spinal segments. immunohistochemical staining supported and refined the latter observation: a dense network of perikarya an ...	1985	3886072
the opioid octapeptide met5-enkephalin-arg6-gly7-leu8: characterization and distribution in rat spinal cord.	the regional quantitation, immunohistochemical localization and molecular heterogeneity of met5-enkephalin-arg6-gly7-leu8 were examined in rat spinal cord with a specific radioimmunoassay. a rostrocaudal gradient in met5-enkephalin-arg6-gly7-leu8 content was observed; the highest levels occurred in sacral cord. dorsal cord content was higher than that of ventral cord at all spinal segments. immunohistochemical staining supported and refined the latter observation: a dense network of perikarya an ...	1985	3886072
ultrastructural demonstration of l-glutamate decarboxylase and cysteinesulfinic acid decarboxylase in rat retina by immunocytochemistry.	the gamma-aminobutyric acid (gaba) synthesizing enzyme, l-glutamate decarboxylase (gad), and the taurine synthesizing enzyme, cysteinesulfinic acid decarboxylase (csad) have been localized in rat retina at the ultrastructural level by indirect immunoelectron microscopy. gad immunoreactivity (gad-ir) was seen only in some amacrine cells and their terminals. csad immunoreactivity (csad-ir) was found in most retinal neuronal types and their processes including photoreceptor cells (rod and cone cell ...	1985	3886078
ultrastructural demonstration of l-glutamate decarboxylase and cysteinesulfinic acid decarboxylase in rat retina by immunocytochemistry.	the gamma-aminobutyric acid (gaba) synthesizing enzyme, l-glutamate decarboxylase (gad), and the taurine synthesizing enzyme, cysteinesulfinic acid decarboxylase (csad) have been localized in rat retina at the ultrastructural level by indirect immunoelectron microscopy. gad immunoreactivity (gad-ir) was seen only in some amacrine cells and their terminals. csad immunoreactivity (csad-ir) was found in most retinal neuronal types and their processes including photoreceptor cells (rod and cone cell ...	1985	3886078
multiple populations of neuropeptide-containing cells in cultures of the bovine adrenal medulla.	cell populations containing vasoactive intestinal polypeptide (vip), enkephalins, and catecholamines were identified in bovine adrenal medullary cultures by immunofluorescence and radioimmunoassay. addition of forskolin to the culture medium increased the cellular levels of both vip and the enkephalins. these changes resulted from an increase in the number of vip-positive cells and an increase in cellular enkephalin content.	1985	3886081
multiple populations of neuropeptide-containing cells in cultures of the bovine adrenal medulla.	cell populations containing vasoactive intestinal polypeptide (vip), enkephalins, and catecholamines were identified in bovine adrenal medullary cultures by immunofluorescence and radioimmunoassay. addition of forskolin to the culture medium increased the cellular levels of both vip and the enkephalins. these changes resulted from an increase in the number of vip-positive cells and an increase in cellular enkephalin content.	1985	3886081
growth factor concentrations and growth-promoting activity in human milk following premature birth.	we have measured growth factor concentrations in human milk from mothers of term and premature infants to identify any adaptive responses to premature delivery. measurements included concentrations of epidermal growth factor and insulin and the growth-promoting activity of milk in vitro, estimated by the stimulation of rats of protein accumulation in cultured human fibroblasts. compared with women delivering at full-term, mothers of premature infants produced milk containing higher concentration ...	1985	3886775
a procedure for light and electron microscopic intracellular immunolocalization of collagen and fibronectin in rat liver.	experimental conditions have been designed that permit both extracellular and intracellular immunolocalization of various collagen types and fibronectin in rat liver. the procedure involves paraformaldehyde fixation by perfusion of the organ, use of saponin as a membrane permeabilizing agent, and visualization of the matrix components by indirect immunoperoxidase. intracellular demonstration of collagens was particularly sensitive to the composition of the fixative and the duration of fixation. ...	1985	3886779
[rare forms of yersinia infections in adults].		1985	3887018
stimulation of dna synthesis in cultured rat alveolar type ii cells.	restoration of the alveolar epithelium after injury is thought to be dependent on the proliferation of alveolar type ii cells. to understand the factors that may be involved in promoting type ii cell proliferation in vivo, we determined the effect of potential mitogens and culture substrata on dna synthesis in rat alveolar type ii cells in primary culture. type ii cells cultured in basal medium containing 10% fetal bovine serum (fbs) exhibited essentially no dna synthesis. factors that stimulate ...	1985	3888615
stimulation of dna synthesis in cultured rat alveolar type ii cells.	restoration of the alveolar epithelium after injury is thought to be dependent on the proliferation of alveolar type ii cells. to understand the factors that may be involved in promoting type ii cell proliferation in vivo, we determined the effect of potential mitogens and culture substrata on dna synthesis in rat alveolar type ii cells in primary culture. type ii cells cultured in basal medium containing 10% fetal bovine serum (fbs) exhibited essentially no dna synthesis. factors that stimulate ...	1985	3888615
subgingival microorganisms and bacterial virulence factors in periodontitis.	considerable information has come forth in recent years on the pathogenic organisms in human periodontitis and the sequence of events by which they produce periodontal disease. important periodontopathogens include bacteroides gingivalis, bacteroides intermedius and actinobacillus actinomycetemcomitans. virulence factors of b. gingivalis and b. intermedius may mainly involve enzymes with potential to interfere with host defenses and to disintegrate periodontal tissues. pathogenic properties of a ...	1985	3890135
passive immunization: a method of enhancing the immune response against antigen mixtures.	antigenic competition is known to be a widespread phenomenon when using crude extracts of antigens (e.g., escherichia coli cytoplasmic proteins) for immunization. this non-specific form of immune suppression can be partially overcome by passive immunization with antibodies against dominant antigens (which are the suppressive molecules) before injection of the antigenic mixture. blocking these immunodominant antigens or antigenic determinants by a passively administered antibody permits antibody ...	1985	3891862
electrophoretic patterns of extracellular deoxyribonuclease (dnase) and their correlation with t-type in group a streptococci.	molecular heterogeneity of the extracellular deoxyribonuclease (dnase) in group a streptococci was demonstrated in 42 clinical isolates. although polyacrylamide gel electrophoretic patterns of the extracellular dnase of all the isolates were heterogeneous, they could be divided into five main patterns with respect to the presence or absence of three dnase components including dnase b. by comparing the electrophoretic patterns of dnase in all the isolates with their t-types, we found that the pat ...	1985	3892240
a review of the enzymic errors in the various porphyrias.		1985	3892647
metastatic capacity of cloned t10 sarcoma cells that differ in h-2 expression: inverse relationship to their immunogenic potency.	clones of the t10 sarcoma, originated in a (h-2b x h-2k)f1 mouse, differ in their metastatic competence, correlated with differences in the expression of antigens of the two parental haplotypes. in fact, the metastatic phenotype is determined by the h-2dk antigen. whether the different major histocompatibility complex gene products control the metastatic phenotype via their different immunogenic properties was tested. the involvement of the immune system in controlling the development of metasta ...	1985	3894752
isolation of a spirochete from the soft tick, ornithodoros coriaceus: a possible agent of epizootic bovine abortion.	a borrelia-like spirochete was detected in all three parasitic stages of ornithodoros coriaceus, the soft tick implicated in the epizoology of epizootic bovine abortion. after the spirochete had been isolated, its distinctness from other north american tick-borne borreliae as well as from spirochaeta aurantia, treponema pallidum, and leptospira interrogans serovar pomona was established on the basis of its morphology, protein components, and inability to infect mice. the spirochete is passed tra ...	1985	3898367
collagen-induced arthritis--use of the direct and indirect haemolytic plaque-assay to study the humoral response to collagen.	using both the direct and indirect haemolytic plaque assay, lymphocytes secreting antibodies to native type ii collagen could be detected in the lymph nodes, spleen and blood of collagen-immunised rats. sprague-dawley, but not alderley park, strain rats showed clear differences in the number of plaque-forming cells in the lymph nodes between arthritic and non-arthritic rats. differences also occurred between these strains in their response to the production of indirect plaques. the presence of n ...	1985	3902297
inactivation of key factors of the plasma proteinase cascade systems by bacteroides gingivalis.	the effect of bacteroides gingivalis w83 on various key components of the human plasma proteinase cascade systems was studied. when purified c1-inhibitor was incubated with the bacterium, the inhibitor was rapidly inactivated by limited proteolytic cleavage. in citrated whole plasma, c1-inhibitor, antithrombin, plasminogen, prekallikrein, prothrombinase complex, the clotting factor x, and most of the alpha 2-antiplasmin were functionally eliminated after 30 min of incubation with the bacterium. ...	1985	3902645
are all recurrences of "pure" sydenham chorea true recurrences of acute rheumatic fever?	we are conducting prospective studies of patients in santiago, chile, who have had an attack of rheumatic fever and are receiving continuous secondary prophylaxis with monthly injections of benzathine penicillin g. throat cultures are obtained just prior to injection each month, and serum antistreptococcal antibody titers (antistreptolysin o and antideoxyribonuclease b) are performed at least every 3 months. during the course of these studies we have observed 17 recurrences of "pure" chorea in 1 ...	1985	3906071
effects of tinoridine on lipid peroxidation and renin release in the rat renin granule fraction.	this study was carried out to investigate the effect of tinoridine (2-amino-3-ethoxycarbonyl-6-benzyl-4,5,6,7-tetrahydrothieno[2,3-c] pyridine), a non-steroidal anti-inflammatory drug, on the lipid peroxidation and renin release in the renin granule fraction. the renin granule fraction was prepared from the kidney cortex homogenate by a discontinuous sucrose density gradient centrifugation. incubation of this fraction at 37 degrees c resulted in an increase in lipid peroxide formation, accompani ...	1985	3906080
gentamicin tympanoclysis: effects on the vestibular secretory cells.	gentamicin application to the middle ear may relieve meniere's disease, presumably by reducing endolymph secretion by the dark cells. to explore this possibility, the ears of adult cats were treated with daily intratympanic infusions of gentamicin until ataxia occurred. the temporal bones of these cats were then examined using electron microscopy. a 3 per cent solution of gentamicin resulted in ataxia after four treatments. acute dark cell damage to basal infoldings seen after one month persiste ...	1985	3907388
[sensitivity of strains of plasmodium falciparum to chloroquine, mefloquine and quinine in 1983 in zaire].		1985	3907531
rapid identification of periodontal pathogens in subgingival dental plaque. comparison of indirect immunofluorescence microscopy with bacterial culture for detection of bacteroides gingivalis.	a large body of research implicates bacteroides gingivalis in the etiology of adult periodontitis, however, the application of this information to clinical diagnosis and treatment has been hampered by the need for a simple, rapid, and reliable means of detecting this microorganism. in the present study, indirect immunofluorescence microscopy using species specific, polyclonal antisera and a monoclonal antibody was evaluated in the clinical identification and quantitation of b. gingivalis in huma ...	1985	3908637
an acoustic plethysmograph to measure total infant body volume.	an acoustic plethysmograph intended to measure the body volume of premature infants has been developed using the principle of the helmholtz resonator, in which the resonance frequency is dependent on the volume of the resonating cavity. a prototype system was built and used to measure the volume of inanimate objects and newborn miniature pigs. results for inanimate objects agree within 1 percent with comparable measurements by water displacement. results of the animal body volume measurements co ...	1985	3908815
antimycotic 2-alkyldithio, 2-aralkyldithio, 2-aryldithiobenzamides.	some 2-alkyldithio, 2-aralkyldithio, 2-aryldithio benzoic acids, their methyl esters and n-monosubstituted amides were prepared and tested in vitro against candida albicans and trichophyton mentagrophytes. some n-monosubstituted amides displayed activities similar to those of clotrimazole and pyrrolnitrin. against candida albicans, n-monosubstituted amides exhibited a generally higher activity than the corresponding n-monosubstituted amides of 2,2'-dicarboxydiphenyldisulfide.	1985	3910452
cefamandole resistance transfer in bacterial strains from two newborn units.	transfer of cefamandole resistance was demonstrated from strains of citrobacter freundii as well as from individual strains of enterobacter cloacae, acinetobacter anitratus and klebsiella pneumoniae isolated from patients in two newborn units. in citrobacter freundii, cefamandole resistance was transferred always with cephalotin resistance as well as with a tem-like beta lactamase (conferring resistance to ampicillin, carbenicillin and azlocillin). citrobacter freundii strains from hospital i we ...	1985	3910723
health effects of nitrogen dioxide and oxidants.	the document is an evaluation of the health effects of nitrogen dioxide (no2) and ozone (o3) intended to serve as a basis for establishing swedish air quality standards. the specific effects of nitrogen dioxide and ozone are reviewed on the basis of published studies on animals, tissues and cells, controlled studies on humans, and epidemiologic studies. the focus is on the importance of the gases in relation to sensitive groups, outdoor-indoor exposure relationships, the question of short- or lo ...	1985	3911379
[effects of nitronaphthofurans on the transfer of a plasmid].		1985	3915195
[aids to wound healing. from empirical tradition to a scientific approach].		1985	3915246
immunology and immunopathology of malignant catarrhal fever.		1985	3917220
proliferative quiescence of normal mast cells resembles that of cold-sensitive mutant mastocytoma cells. dominant expression of the quiescent state in heterokaryons.	normal murine peritoneal mast cells were fused to serum-deprived, non-proliferating cells of a cultured subline (41-sb-4) of the p-815 murine mastocytoma. upon reincubation in medium containing 10% horse serum for 48 h, mono- and binuclear 41-sb-4 cells reentered s phase of the cell cycle, while mast cell x 41-sb-4 heterokaryons as well as mono- and binuclear mast cells remained in proliferative quiescence, indicating dominant expression of the quiescent state of mast cells. the quiescent state ...	1985	3922778
the urinary excretion of bacterial amino-acid metabolites by rats fed saccharin in the diet.	administration of a diet containing 7.5% saccharin to adult male rats for 40 days caused a three- to four-fold increase in the daily excretion of indican and rho-cresol. indican is formed from indole which is a microbial metabolite of tryptophan, whilst rho-cresol is formed by the gut flora from tyrosine. the excretion of phenol, which is also a microbial metabolite of tyrosine, was abolished by saccharin administration for 40 days. analysis of urines collected at 13, 18 and 24 months during a t ...	1985	3924804
inhibition of lymphocyte invasion by sporozoites and the transformation of trophozoite infected lymphocytes in vitro by serum from theileria annulata immune cattle.	serum from calves immune to theileria annulata inhibited sporozoite-induced infection and transformation of peripheral blood leucocytes (pbl) into macroschizont infected lymphoblastoid cells in vitro. invasion by sporozoites was suppressed by some sera, especially that obtained after challenge, but in many instances suppression of infection could be attributed only to retardation of development of pbl infected with intracellular trophozoites into proliferating macroschizont infected cells. suppr ...	1985	3925422
glycosaminoglycan-depolymerizing enzymes produced by anaerobic bacteria isolated from the human mouth.	a number of obligately anaerobic bacteria, some implicated in periodontal disease, were screened for their ability to produce enzymes capable of degrading hyaluronic acid and chondroitin-4-sulphate. two screening methods were used following anaerobic incubation at 37 degrees c for 7 days. one involved incorporating the respective substrates and bovine-serum albumin into agar plates and, after incubation, flooding the plates with 2 m acetic acid. clear zones were produced around colonies which pr ...	1985	3927877