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[characteristics of adhesion of epiphytic bacteria on leaves of the seagrass zostera marina and on abiotic surfaces].a comparative study of the adhesion of epiphytic bacteria and marine free-living, saprophytic, and pathogenic bacteria on seagrass leaves and abiotic surfaces was performed to prove the occurrence of true epiphytes of zostera marina and to elucidate the bacterium-plant symbiotrophic relationships. it was shown that in the course of adhesion to the seagrass leaves of two taxonomically different bacteria, cytophaga sp. kmm 3552 and pseudoalteromonas citrea kmm 461, isolated from the seagrass surfa ...200717974207
genomic plasticity of the rrn-nqrf intergenic segment in the chlamydiaceae.in chlamydiaceae, the nucleotide sequence between the 5s rrna gene and the gene for subunit f of the na(+)-translocating nadh-quinone reductase (nqrf or dmpp) has varied lengths and gene contents. we analyzed this site in 45 chlamydiaceae strains having diverse geographical and pathological origins and including members of all nine species.200717158668
rapid detection of vibrio species using liquid microsphere arrays and real-time pcr targeting the ftsz locus.the development of rapid and sensitive molecular techniques for the detection of vibrio species would be useful for the surveillance of sporadic infections and management of major outbreaks. comparative sequence analysis of the ftsz gene in the predominant vibrio species that cause human disease revealed distinct alleles for each examined species, including vibrio cholerae, vibrio parahaemolyticus and vibrio vulnificus. light upon extension (lux) real-time pcr assays were developed to target the ...200717172518
the immunostimulatory effects of hot-water extract of gelidium amansii via immersion, injection and dietary administrations on white shrimp litopenaeus vannamei and its resistance against vibrio alginolyticus.the total haemocyte count (thc), phenoloxidase activity, and respiratory burst were examined when white shrimp litopenaeus vannamei were immersed in seawater (34 per thousand) containing hot-water extract of red alga gelidium amansii at 200, 400 and 600 mg l(-1), injected with hot-water extract at 4 and 6 microg g(-1) shrimp, and fed diets containing hot-water extract at 0, 0.5, 1.0 and 2.0 g kg(-1). these parameters increased significantly when shrimp were immersed in seawater containing hot-wa ...200717194604
identification and cloning of the antioxidant enzyme, glutathione peroxidase, of white shrimp, litopenaeus vannamei, and its expression following vibrio alginolyticus infection.cdna encoding glutathione peroxidase (gpx) mrna of the white shrimp litopenaeus vannamei was obtained from haemocytes by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna (race) using oligonucleotide primers based on the gpx sequence of homo sapiens (nm002083), mus musculus (nm008160), arabidopsis thaliana (u94495), bos taurus (nm174770), and capsicum chinense (aj973135). the 727-bp cdna contained an open reading frame (orf) of 567 bp, a 101-bp 5'-untrans ...200717208011
hedistin: a novel antimicrobial peptide containing bromotryptophan constitutively expressed in the nk cells-like of the marine annelid, nereis diversicolor.a novel antimicrobial peptide, named hedistin was identified from the coelomocytes of nereis diversicolor. hedistin shows no obvious similarities with other known peptides and constitutes the first antimicrobial peptide containing bromotryptophans demonstrated in annelids. cdna and mass spectrometry analysis revealed that, upon bacteria challenge, this peptide is secreted following processing of a precursor containing a signal peptide and prosequences. hedistin was shown to possess an activity a ...200717210178
effect of sulfide on the immune response and susceptibility to vibrio alginolyticus in the kuruma shrimp marsupenaeus japonicus.kuruma shrimp marsupenaeus japonicus held in 34 per thousand seawater were injected with tryptic soy broth (tsb)-grown vibrio alginolyticus (2.7x10(6)cfu shrimp(-1)), and then placed in water containing concentrations of sulfide at 0 (control), 51, 106, 528 and 1050microgl(-1), respectively. after 12-144h, mortality of v. alginolyticus-injected shrimp exposed to 528 and 1102microgl(-1) sulfide was significantly higher than that of shrimp exposed to 51microgl(-1) sulfide and the control solution. ...200716713303
dietary administration of sodium alginate and kappa-carrageenan enhances the innate immune response of brown-marbled grouper epinephelus fuscoguttatus and its resistance against vibrio alginolyticus.brown-marbled grouper epinephelus fuscoguttatus which had been fed diets containing sodium alginate and kappa (kappa)-carrageenan at 5, 10 and 20gkg(-1), respectively after 0, 2, 4, 6, 8 and 14 weeks were examined for survival, growth, innate cellular and humoral responses as compared to the fish that fed control non-supplemented diet. survival was 100% for the fish that fed all diets after 14 weeks and no significant difference in growth was observed among seven diets. the fish that fed a diet ...200817981341
effect of saponin immersion on enhancement of the immune response of white shrimp litopenaeus vannamei and its resistance against vibrio alginolyticus.the haemocyte count, phenoloxidase (po) activity, specific alpha(2)-macroglobulin (alpha2-m) activity, respiratory burst, superoxide dismutase (sod) activity, glutathione peroxidase (gpx) activity, phagocytic activity, and clearance efficiency against vibrio alginolyticus were examined when the white shrimp litopenaeus vannamei (10.42+/-2.0g) were immersed in seawater (34 per thousand) containing different concentrations of saponin (0, 0.5, 1 and 2mgl(-1)) for 24, 48 and 72h. hyaline cells (hc), ...200818032066
molecular cloning of leucocyte cell-derived chemotaxin-2 gene in croceine croaker (pseudosciaena crocea).leucocyte cell-derived chemotaxin-2 (lect2) was originally demonstrated to have a chemotactic activity against human neutrophils in vitro. current evidence suggests that lect2 may be a multifunctional protein involved in cell growth, differentiation and autoimmune. a full-length cdna clone of the lect2 gene, 595bp in size, was isolated from the fish croceine croaker (pseudosciaena crocea). it's 3'-utr was much shorter (112nts) than that of trout lect2 gene (210nts). its deduced amino acid sequen ...200818155922
complement-mediated killing of vibrio species by the humoral fluids of amphioxus branchiostoma belcheri: implications for a dual role of o-antigens in the resistance to bactericidal activity.the functional properties of complement in invertebrate deuterostomes are rather ill-defined. here we showed that the humoral fluids from amphioxus branchiostoma belcheri were capable of causing lysis of some vibrio species including vibrio alginolyticus hw284, vibrio parahaemolyticus hw458 and vibrio harvey sf-1, the first such data in the invertebrate deuterostomes. the fluid bacteriolytic activity was abolished by pre-incubation with heat-inactivated rabbit anti-human c3 serum, heating at 45 ...200818178103
torque-speed relationships of na+-driven chimeric flagellar motors in escherichia coli.the bacterial flagellar motor is a rotary motor in the cell envelope of bacteria that couples ion flow across the cytoplasmic membrane to torque generation by independent stators anchored to the cell wall. the recent observation of stepwise rotation of a na(+)-driven chimeric motor in escherichia coli promises to reveal the mechanism of the motor in unprecedented detail. we measured torque-speed relationships of this chimeric motor using back focal plane interferometry of polystyrene beads attac ...200818207160
vibrios in association with sedimentary crustaceans in three beaches of the northern adriatic sea (italy).in the marine environment, vibrios adhere to a number of substrates including chitin-rich organisms such as crustaceans. their wide diffusion in coastal waters and pathogenic potential require knowledge of the lifestyle and environmental reservoirs of these bacteria. to test the presence of culturable vibrios in coastal areas and their association with benthic crustaceans, vibrios were isolated from water, sediments and crustaceans (copepods and anphipods) at three stations placed in front of he ...200818243247
regulation of vibrio alginolyticus virulence by the luxs quorum-sensing system.quorum sensing (qs) is a bacterial intercommunication system that controls the expression of multiple genes in response to population density. the luxs qs system regulates the expression of several virulence factors in a wide variety of pathogenic bacteria. luxs has been characterized to be responsible for producing a type of autoinducer, ai-2, which stimulates the expression of the luciferase operon in vibrio harveyi. vibrio alginolyticus is established as an opportunistic pathogen of several m ...200818261029
the na+-translocating nadh:quinone oxidoreductase (na+-nqr) from vibrio cholerae enhances insertion of fes in overproduced nqrf subunit.the na+-translocating nadh:quinone oxidoreductase (na+-nqr) from vibrio cholerae is a membrane-bound, respiratory na+ pump. its nqrf subunit contains one fad and a [2fe-2s] cluster and catalyzes the initial oxidation of nadh. a soluble variant of nqrf lacking its hydrophobic, n-terminal helix (nqrf') was produced in v. cholerae wild type and nqr deletion strain. under identical conditions of growth and induction, the yield of nqrf' increased by 30% in the presence of the na+-nqr. fad-containing ...200818289689
environmental vibrio spp., isolated in mozambique, contain a polymorphic group of integrative conjugative elements and class 1 integrons.circulation of mobile genetic elements linked to drug resistance spread was studied in vibrio strains isolated from surface urban water (river and sea) and shellfish samples in 2002-2003 in maputo, mozambique. class 1 integrons and integrating conjugative elements (ice) were investigated by pcr and mating experiments in strains of major health interest: 10 vibrio cholerae, six vibrio parahaemolyticus, two vibrio alginolyticus and one vibrio fluvialis. resistance to at least two antibiotics (pred ...200818318712
roles of charged residues in the c-terminal region of poma, a stator component of the na+-driven flagellar motor.bacterial flagellar motors use specific ion gradients to drive their rotation. it has been suggested that the electrostatic interactions between charged residues of the stator and rotor proteins are important for rotation in escherichia coli. mutational studies have indicated that the na(+)-driven motor of vibrio alginolyticus may incorporate interactions similar to those of the e. coli motor, but the other electrostatic interactions between the rotor and stator proteins may occur in the na(+)-d ...200818326582
influence of beta-glucans on the immune responses of carpet shell clam (ruditapes decussatus) and mediterranean mussel (mytilus galloprovincialis).the effects of beta-glucans on several immune functions of carpet shell clam (ruditapes decussatus) and mediterranean mussel (mytilus galloprovincialis) hemocytes were determined. nitric oxide (no) production increased significantly in beta-glucan treated mussels and clams. in mussels, beta-glucans increased by themselves the release of free oxygen radicals and also were able to enhance the phorbol 12-myristate 13-acetate (pma) mediated effect on this hemocyte activity. however, high doses of be ...200818329901
a novel c-type lectin from abalone, haliotis discus discus, agglutinates vibrio alginolyticus.owing to its specific binding to carbohydrates, lectins play important roles in pathogen recognition and clearance in invertebrate animals. in this study, a novel c-type lectin (designated clhd) gene was isolated from abalone, haliotis discus discus, cdna library. the complete cdna sequence of the clhd gene is 508 base pairs in length, and encodes 151 amino acids. clhd shares a highly conserved carbohydrate recognition domain with c-type lectins from mollusk and fish. the mrna expressions of clh ...200818372041
[cutaneous ulceration after an octopus bite: infection due to vibrio alginolyticus, an emerging pathogen].vibrio alginolyticus is an anaerobic gram-negative bacillus found in normal marine flora and is considered as non pathogenic for humans. infections due to vibrio alginolyticus are seldom reported and can be seen after exposure to seawater or to marine animals.200818374857
involvement of luxs in the regulation of motility and flagella biogenesis in vibrio alginolyticus.the fish pathogen vibrio alginolyticus contains two unique flagellar systems. the luxs quorum sensing system is reported to regulate the expression of virulence factors in a wide variety of pathogenic bacteria. our previous work demonstrated that inactive luxs led to decreased virulence in v. alginolyticus. in this study, luxs-dependent regulation of motility and flagella biogenesis, the potential virulence factors in v. alginolyticus, were further investigated. a luxs-deleted mutant showed defi ...200818391446
collaboration of flhf and flhg to regulate polar-flagella number and localization in vibrio alginolyticus.precise regulation of the number and placement of flagella is critical for the mono-polar-flagellated bacterium vibrio alginolyticus to swim efficiently. we have shown previously that the number of polar flagella is positively regulated by flhf and negatively regulated by flhg. we now show that deltaflhf cells are non-flagellated as are most deltaflhfg cells; however, some of the deltaflhfg cells have several flagella at lateral positions. we found that flhf-gfp was localized at the flagellated ...200818451048
septic shock due to vibrio alginolyticus in a cirrhotic patient: the first case in korea.we describe a case of septic shock due to vibrio alginolyticus presenting with fever and bilateral leg pain. despite intensive management with antibiotics and inotropic agents, the patient died from septic shock 1 day after hospitalization. v. alginolyticus was isolated from both leg wounds and a blood culture. to the best of our knowledge, this is the first reported case of v. alginolyticus bacteremia in korea.200818452273
insights into the stator assembly of the vibrio flagellar motor from the crystal structure of moty.rotation of the sodium-driven polar flagellum of vibrio alginolyticus requires four motor proteins: poma, pomb, motx, and moty. poma and pomb form a sodium-ion channel in the cytoplasmic membrane that functions as a stator complex to couple sodium-ion flux with torque generation. motx and moty are components of the t-ring, which is located beneath the p-ring of the polar flagellar basal body and is involved in incorporation of the poma/pomb complex into the motor. here, we describe the determina ...200818505842
chemical investigation of finlaysonia obovata: part i--a rare triterpene acid showing antibacterial activity against fish pathogens.the antibacterial screening of extracts of the leaves of finlaysonia obovata with hexane, chloroform and alcohol was carried out against fresh water fish pathogenic bacteria viz., micrococcus sp. (multidrug resistant strain), aeromonas hydrophila, pseudomonas aeruginosa, vibrio alginolyticus, staphylococcus aureus, escherichia coli, edwardsiella tarda by disc-assay method. the hexane and chloroform extracts were found active against four and five pathogens, respectively. the highly active chloro ...200818569719
roles of luxr in regulating extracellular alkaline serine protease a, extracellular polysaccharide and mobility of vibrio alginolyticus.in marine vibrio species, the vibrio harveyi-type luxr protein, a key player in a quorum-sensing system, controls the expression of various genes. in this study, the luxr homologue in vibrio alginolyticus was identified and named luxr(val), whose expression was greatly induced by the increase of cell number. the luxr(val) in-frame deletion mutant showed a significant downregulation of total extracellular protease activity, and especially caused a 70% decrease in the transcript levels of extracel ...200818573155
genotyping of vibrio alginolyticus isolates from daya bay by infrequent-restriction-site pcr and pulsed-field gel electrophoresis.vibrio alginolyticus is a serious bacterial pathogen that hampered the whole industry of fish farming in guangdong, china. in order to facilitate epidemiologic studies and improve the control of disease, we developed a highly efficient method of genotyping for v. alginolyticus using infrequent-restriction-site pcr (irs-pcr) technology. with the enzyme combination of noti-hhai, optimized, unique, and easy-to-interpret patterns were generated. forty-five v. alginolyticus isolates from aquatic anim ...200818586455
characterization of two tonb systems in marine fish pathogen vibrio alginolyticus: their roles in iron utilization and virulence.in gram-negative bacteria, the tonb/exbb/exbd complex is required to energize the specific high-affinity receptors mediating iron uptake processes. in fish pathogen vibrio alginolyticus mvp01, which exhibited capacities to scavenge iron sources with various specific iron uptake systems, we identified and characterized two sets of tonb systems. in v. alginolyticus, tonb1 and tonb2 systems were arrayed as tonb1-exbb1-exbd1 and exbb2-exbd2-tonb2, respectively. the transcription of the tonb systems ...200818629473
role of rpos in stress survival, synthesis of extracellular autoinducer 2, and virulence in vibrio alginolyticus.vibrio alginolyticus, a marine bacterium, is an opportunistic pathogen capable of causing vibriosis with high mortality to fishes in the south china sea. stress resistance is very important for its survival in the natural environment and upon infection of the host. rpos, an alternative sigma factor, is considered as an important regulator involved in stress response and virulence in many pathogens. in this study, the rpos gene was cloned and characterized to evaluate the role of rpos in v. algin ...200818641971
vibrios dominate as culturable nitrogen-fixing bacteria of the brazilian coral mussismilia hispida.taxonomic characterization was performed on the putative n(2)-fixing microbiota associated with the coral species mussismilia hispida, and with its sympatric species palythoa caribaeorum, p. variabilis, and zoanthus solanderi, off the coast of são sebastião (são paulo state, brazil). the 95 isolates belonged to the gammaproteobacteria according to the 16s rdna gene sequences. in order to identify the isolates unambiguously, pyrh gene sequencing was carried out. the majority of the isolates (n = ...200818678453
effects of rutin from toona sinensis on the immune and physiological responses of white shrimp (litopenaeus vannamei) under vibrio alginolyticus challenge.rutin is a bioflavonoid with strong antioxidant activity. to investigate the regulatory roles of rutin in various functions in crustaceans, we examined physiological (haemolymph glucose, lactate, and lipid) and innate non-specific immune responses (total haemocyte count (thc), phenoloxidase activity (po), respiratory bursts (release of superoxide anion, o(2)(-)) and superoxide dismutase (sod) activity) to the pathogen vibrio alginolyticus in white shrimp (litopenaeus vannamei) that were individu ...200818718869
[isolation and physiological characteristics of lytic bacteriophages of vibrio].vibrio is a widely distributed pathogen in aquatic environment. our study aimed at searching for possible biological control of pathogenic vibrio.200818720843
transcriptional up-regulation of disk abalone selenium dependent glutathione peroxidase by h(2)o(2) oxidative stress and vibrio alginolyticus bacterial infection.selenium dependent glutathione peroxidase (se-gpx) belongs to the family of selenoprotein, which acts mainly as an antioxidant in the cellular defence system. we have identified se-gpx full length cdna from disk abalone (haliotis discus discus) designated as abse-gpx. it has a characteristic codon at (223)tga(225) that corresponds to selenocysteine (sec) amino acid as u(75). the full length cdna consists of 675 bp, an open reading frame encoding 225 amino acids. sequence characterization reveale ...200818722791
enzymatic assay of marine bacterial phosphatases by capillary electrophoresis with laser-induced fluorescence detection.microbial ectoenzyme activities in aquatic environments are important determinants of polymer hydrolysis and indicators of the state of microbial carbon, nitrogen, and phosphorus nutrition. marine ectoenzymes are found on the cell surface or in the periplasmic space of gram-negative heterotrophic bacteria. phosphatases, which remove phosphate groups from substrates, are one example of an ectoenzyme. enzyme assays based on-capillary electrophoresis (ce) take advantage of ce's high-efficiency sepa ...200818726971
cell-free synthesis of the torque-generating membrane proteins, poma and pomb, of the na+-driven flagellar motor in vibrio alginolyticus.flagellar motor proteins, poma and pomb, are essential for converting the sodium motive force into rotational energy in the na(+)-driven flagella motor of vibrio alginolyticus. poma and pomb, which are cytoplasmic membrane proteins, together comprise the stator complex of the motor and form a na(+) channel. we tried to synthesize poma and pomb by using the cell-free protein synthesis system, puresystem. we succeeded in doing so in the presence of liposomes, and showed an interaction between them ...200818776205
molecular characterization and gene expression analysis of a pattern recognition protein from disk abalone, haliotis discus discus.pattern recognition molecules play an important role in innate immunity by recognizing common epitopes on the surface of invading microorganism. a pattern recognition protein (prp) was isolated from a disk abalone, haliotis discus discus, normalized cdna library. it encodes 420 amino acids (aa) including a 20 aa signal peptide sequence. the mature protein has an estimated molecular mass of 45kda and predicted pi of 5.0. the deduced aa sequence showed greatest homology (50%) with a beta-glucan re ...200818782621
expression and characterization of the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from vibrio alginolyticus.the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase (pepd) gene from vibrio alginolyticus was cloned and sequenced. the recombinant pepd protein was produced and biochemically characterized and the putative active-site residues responsible for metal binding and catalysis were identified. the recombinant enzyme, which was identified as a homodimeric dipeptidase in solution, exhibited broad substrate specificity for xaa-his and his-xaa dipeptides, with the highest activity ...200818783432
the immune response of white shrimp litopenaeus vannamei and its susceptibility to vibrio alginolyticus under low and high ph stress.white shrimp litopenaeus vannamei (also known as penaeus vannamei) held in 34 per thousand seawater at ph 8.2 were injected with tryptic soy broth (tsb)-grown vibrio alginolyticus at 8 x 10(5) colony-forming units (cfu) shrimp(-1), and then transferred to tanks at ph 6.5, 8.2 (control) and 10.1, respectively. after 24-168 h, the mortality of v. alginolyticus-injected shrimp that were transferred to ph 6.5 and ph 10.1 tanks was significantly higher than that of v. alginolyticus-injected shrimp he ...200818990589
pacemaker infection due to vibrio alginolyticus. 200819046705
flagellar motility in bacteria structure and function of flagellar motor.bacterial flagella are filamentous organelles that drive cell locomotion. they thrust cells in liquids (swimming) or on surfaces (swarming) so that cells can move toward favorable environments. at the base of each flagellum, a reversible rotary motor, which is powered by the proton- or the sodium-motive force, is embedded in the cell envelope. the motor consists of two parts: the rotating part, or rotor, that is connected to the hook and the filament, and the nonrotating part, or stator, that co ...200819081534
the vibrio core group induces yellow band disease in caribbean and indo-pacific reef-building corals.to determine the relationship between yellow band disease (ybd)-associated pathogenic bacteria found in both caribbean and indo-pacific reefs, and the virulence of these pathogens. ybd is one of the most significant coral diseases of the tropics.200818798767
enzymatic characterization of vibrio alginolyticus strains isolated from bivalves harvested at venice lagoon (italy) and guanabara bay (brazil).the aquatic ecosystem is the natural habitat of microorganisms including vibrio and aeromonas genus which are pathogenic to human and animals. in the present investigation the frequency of these bacteria and the enzymatic characteristics of 34 vibrio alginolyticus strains isolated from bivalves harvested in venice lagoon (italy) and guanabara bay (brazil) were carried out from november 2003 to february 2004. the mussels' samples were submitted to enrichment in alkaline peptone water (apw) added ...200818813756
portal of entry for pathogenic vibrio alginolyticus into large yellow croaker pseudosciaena crocea, and characteristics of bacterial adhesion to mucus.the portal of entry for pathogenic vibrio alginolyticus into large yellow croaker pseudosciaena crocea is via the intestinal tract rather than gill or skin according to the kinetics of the bacterial adhesion to different mucus. the different effects on adhesion caused by proteolytic enzymes and heat treatment might be due to the different chemical compositions of mucus. adhesion of v. alginolyticus to mucus depends on concerted action of bacterial surface structures such as cell-surface proteins ...200818814543
molecular and functional characterization of a novel cc chemokine in large yellow croaker (pseudosciaena crocea).a full-length cc chemokine cdna was isolated from large yellow croaker (pseudosciaena crocea) by expressed sequence tag (est) analysis (lyccc2). its open reading frame (orf) is 282 nucleotides (nt), encoding a polypeptide of 93 amino acids (aa). the deduced lyccc2 contains a 20-aa signal peptide and a 73-aa mature polypeptide, which possesses the typical arrangement of four cysteines as found in other known cc chemokines (c(34), c(35), c(61) and c(75)). it shares 17.4-40.4% and 10.0-24.5% aa seq ...200818835451
the effect of vibrio alginolyticus infection on caspase-3 expression and activity in white shrimp litopenaeus vannamei.complementary (c)dna encoding cysteine aspartate protease-3 (caspase-3) messenger (m)rna of the white shrimp litopenaeus vannamei was obtained from haemocytes by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) using oligonucleotide primers based on the caspase sequences of penaeus merguiensis, spodoptera frugiperda, spodoptera littoralis, and penaeus monodon. the 1212-bp cdna contained an open reading frame (orf) of 951bp, a 56-bp 5'-untrans ...200818840531
the immune response of white shrimp litopenaeus vannamei following vibrio alginolyticus injection.white shrimp litopenaeus vannamei injected with saline, and injected with tryptic soy broth (tsb)-grown vibrio alginolyticus at 1.0 x 10(5) and 1.8 x 10(5) colony-forming units (cfu) shrimp(-1) were examined for hyaline cell (hc) counts, granular cell (gc) counts, total haemocyte counts (thcs), phenoloxidase (po) activity, respiratory burst (rb) and superoxide dismutase (sod) activity after 1-168 h. shrimp that received no injection served as the control. the shrimps which received v. alginolyti ...200818951981
characterization of the bpi-like gene from a subtracted cdna library of large yellow croaker (pseudosciaena crocea) and induced expression by formalin-inactivated vibrio alginolyticus and nocardia seriolae vaccine challenges.one expressed sequence tag (est 64lf004 clone), which is from the subtracted cdna library of the head kidney of large yellow croaker (pseudosciaena crocea) stimulated with peptidoglycan (pg) by suppression subtractive hybridization (ssh) method, was cloned using race-pcr. the full length cdna, which possesses typical structural features of a signal peptide, a conserved lps binding domain and two bactericidal permeability-increasing (bpi) motifs as in higher vertebrates, was identified as a novel ...200818952461
extraintestinal vibrio infections in mauritius.few extraintestinal vibrio infections have been reported in the african region. we report 3 cases from mauritius: one case of vibrio alginolyticus otitis externa; one case of soft tissue infection caused by non-o1 vibrio cholerae and vibrio parahaemolyticus; and one fatal case of non-o1 v. cholerae cellulitis and septicaemia.200819745511
adhesive properties of environmental vibrio alginolyticus strains to biotic and abiotic surfaces.the ability of vibrio alginolyticus strains isolated from a bathing and fishing area (khenis, centre of tunisia) to adhere to both biotic and abiotic surfaces was evaluated in the present work. the biochemical, physiological and enzymatic activities of all strains was also investigated. three morphotypes of v. alginolyticus were obtained on congo red agar and only 14 strains produced black colonies. the majority of strains were able to degrade the skin mucus of both sparus aurata and dicentrarch ...200819123304
vibrio alginolyticus gyrb sequence analysis and gyrb-targeted pcr identification in environmental isolates.gyrb fragments (about 1.2 kb) of 9 vibrio alginolyticus strains were sequenced, and their phylogenetic relationship with other closely related vibrio species was analyzed. all the v. alginolyticus strains grouped into one strongly supported cluster in the phylogenetic tree. there were 54 base variations among the 1167 bp mutual gyrb regions of 11 v. alginolyticus strains; all the v. alginolyticus strains shared the same amino acid sequence except v. alginolyticus atcc 17749. based on the gyrb se ...200819244972
cystic neoplasms of the pancreas.eight patients with cystic neoplasms of the pancreas were seen at four northern california hospitals between the years 1978 and 1986. three of the tumors were benign and five were malignant. three females, whose average age was 61 years, had cystadenomas. three females and two males, whose average age was 48 years, had mucinous cystadenocarcinomas. clinical presentations were similar among all patients. abdominal pain was a prominent feature. anorexia, weight loss, nausea and vomiting with a pal ...20082446398
transboundary metal pollution of the columbia river (franklin d. roosevelt lake). 20082265269
mental ability, symbolic play and receptive and expressive language of young children with down's syndrome.the relationship between measures of mental ability, symbolic play and expressive and receptive language was investigated in a sample of 73 children with down's syndrome (mean ca 48.9 months, range 19-90 months; mean ma 30.4 months, range 13-73 months). language delay became increasingly apparent with age, and more so in boys than girls. there was no significant difference between mental ages derived from standard ability tests and corresponding age equivalent scores of the lowe and costello sym ...20082579964
[measurement of orthodontic appliance force using prototype sensors].in introductory remarks the most important biomechanical principles, which are relevant for the research, and conditions which must be satisfied presented in intraoral measuring of orthodontic forces were pointed out. first the aim of the research was defined, and then the investigation of mechanical properties of acrylics as a material for orthodontic appliances were carried out. the type of strain gauges, bonds and measuring system configurations, as well as measuring amplifiers and recording ...20082635572
cloning and expression of an actin gene in the haemocytes of pearl oyster (pinctada fucata, gould 1850).an actin gene (designated pfact1) of pearl oyster, pinctada fucata, was cloned from haemocytes by the techniques of homological cloning and rapid amplification of cdna ends (race). the full length of pfact1 cdna was 1608-ábp in length, having a 5' untranslated region (utr) of 82-ábp, a 3' utr of 395-ábp, and an open reading frame (orf) of 1131-ábp encoding a polypeptide of 376 amino acids with a predicted molecular weight of 41.76-ákda and an estimated isoelectric point of 5.29. sequence analysi ...200821798155
reflections on tissue characterisation.theoretical predictions that absorption and velocity dispersion, in addition to characteristic acoustic impedance mismatch, mediate the reflectivity of a planar interface are explored. a technique to measure the frequency dependence of the reflectivity is described: a special hydrophone, purpose-built to this end, is utilised in order to show that perspex/water and neoprene/water reflectivities are not inconsistent with the general predictions of the theory. an approach towards displaying, simul ...20082587629
the paraphilias and depo-provera: some medical, ethical and legal considerations.paraphilic disorders are axis i psychiatric afflictions. they are not acquired by volitional decision, but are manifested by the association of erotic arousal with unacceptable behavior or stimulae (e.g., children). because paraphilic behavior occurs in the service of a biological drive, use of medication to suppress sexual appetite may constitute an adjunct in treatment. medroxyprogesterone can be used to decrease unacceptable erotic urges and fantasies, with the intent of increasing self-contr ...20082529006
the biology of the scid mutation. 20082519515
testis position and age at orchidopexy. 20082885626
[alpha-amylase polymorphism. 1. a comparative study of alpha-amylase hp and gm].individual phenotypes, phenotypical and genetic frequencies of the alpha-amylase enzyme have been established by means of populational genetic researches. the most common phenotype is amyla amyl2a (85.15%) followed by amyla amyl2a 2b (6.27%), amylaib amyl2a (5.37%), amyl ia amyl2a 2b (2.15%), amyla amyl2b (0.53%), amylc amyl2b (0.35%), amylc amyl2a 2b (0.18%). the difference between the observed and theoretically expected phenotypes of amy, hp and m gm(1) is insignificant. the examined contingen ...20082627896
colony-stimulating factor and drug-induced agranulocytosis. 20082655524
colony-stimulating factor and drug-induced agranulocytosis. 20082655524
[humeroscapular periarthritis]. 20082620726
[humeroscapular periarthritis]. 20082620726
vascular myogenic or stretch-dependent tone.myogenic tone is an important source of vascular resistance, which appears to depend upon stretch of the vessel wall. it contributes to autoregulation, is dependent upon external calcium, and may involve a calcium-entry mechanism different from that associated with the action of agonists and changes in the membrane potential.20082409386
evaluating the optometric literature: the educated clinician.the purpose of this article is to present a framework for reader evaluation of optometric literature. the elements of a research article are outlined. suggestions are presented that clinicians can use to develop competence in evaluating research literature. also presented is an annotated bibliography of research references. better patient care and increased professional growth will result when clinicians evaluate and make use of the research literature.20082703662
evaluating the optometric literature: the educated clinician.the purpose of this article is to present a framework for reader evaluation of optometric literature. the elements of a research article are outlined. suggestions are presented that clinicians can use to develop competence in evaluating research literature. also presented is an annotated bibliography of research references. better patient care and increased professional growth will result when clinicians evaluate and make use of the research literature.20082703662
[gypsum-bonded investments for the ringless casting (part 1). the mechanical properties of the experimental investments]. 20082699669
[gypsum-bonded investments for the ringless casting (part 1). the mechanical properties of the experimental investments]. 20082699669
[histochemical study of amyloid spondyloarthropathy in hemodialyzed patients]. 20082657995
organising clinical research: 2. writing the protocol. 20092803899
computer-assisted semen analysis: results vary across technicians who prepare videotapes.automated semen analyses revealed differences of 21% to 30% in concentration-related parameters and 5% to 11% in motion-related parameters between means of groups of replicate specimens. disparities among videotapes produced by two laboratory technicians accounted for the divergence in concentration-related parameters. this resulted partially from differences between the two technicians in propensity to dilute concentrated specimens. the causes of the greater portion of disparities between video ...20092680623
the maillard reaction in aging, diabetes, and nutrition. proceedings of an nih conference. bethesda, maryland, september 22-23, 1988. dedicated to john e. hodge on the occasion of his 75th birthday. 20092675025
chernobyl and cancer epidemiology. 20092882249
lack of nephrotoxicity of styrene at current tlv level (50 ppm).biochemical markers of kidney damage were examined in 37 female workers exposed to an average concentration of 225 mg/m3 of styrene. the concentration of mandelic acid in urine was on the average 759 mg/g creatinine. the mean duration of employment of the exposed subjects was 11 years. the results were compared to those obtained in 35 control female workers matched for age and a number of demographic and lifestyle factors and with no history of exposure to organic solvents. no difference was fou ...20092744872
predictive value of a short dietary questionnaire for changes in serum lipids in high-risk utah families.dietary questionnaires and serum cholesterol, triglycerides, and high-density lipoprotein (hdl) cholesterol determinations were completed for 1239 subjects aged greater than or equal to 20 at each of two separate screenings. the mean time between screenings was 2.5 y. after correcting for potential confounding variables, reduction of a measure of dietary cholesterol and saturated fatty acids assessed by two simple questions was a significant independent predictor of reduction in total cholestero ...20092756916
renal adenocarcinoma presenting with bilateral metastases to bartholin's glands: primary diagnosis by aspiration cytology.the first recorded case of renal adenocarcinoma presenting with bilateral metastases to the bartholin's glands is presented. primary diagnosis of the metastatic deposits was made by fine-needle aspiration cytology with subsequent localization of the unsuspected renal primary. the value of fine-needle aspiration in the identification of inapparent primary tumors is discussed and cytological findings are described in detail.20093769735
[microcomputer-assisted system for photographing the movements of biological valve prostheses].the aim of the study was to develop a clinically usable system of photographic imaging of the movements of biological valve transplants and of evaluating temporal and spatial relationships. the system has been established by means of a microcomputer and associated peripheral electronic equipment so that there is no need to use a high-speed camera. functioning of the system was verified by testing 37 aortal valve allotransplants and the björk shiley mechanical valve prosthesis.20092758334
aids: not the only infectious killer. 20092761978
recurrent anorexia nervosa in a hypercholesterolemic woman treated with exercise and diet modification. 20092725596
[conventional insulin therapy, infusion pumps, injector syringes. different methods of administration]. 20092665113
[poisoning by geromitra esculenta].gyromitra esculenta (persoon ex fries) mushrooms have been responsible for severe intoxications and even deaths. clinical data are characterized primarily by vomiting and diarrhoea, and, afterwhile, by jaundice, convulsions and coma. the species of concern are mainly g. esculenta, g. fastigiata and g. gigas; nevertheless, recent advances in chromatography, biochemistry and toxicology have established that other species within the ascomycetes may prove also toxic. the toxins, i.e. gyromitrin (n-m ...20092681713
sonographic evaluation of superior mesenteric vascular relationship in malrotation.we report sonographic demonstration of abnormal and characteristic superior mesenteric vascular anatomy in three cases of surgically proved intestinal malrotation. sonographic screening examinations of children with abdominal pain should include an evaluation of the superior mesenteric vessels.20092654854
comparative study of the ion flux pathway in stator units of proton- and sodium-driven flagellar motors.flagellar motor proteins, mota/b and poma/b, are essential for the motility of escherichia coli and vibrio alginolyticus, respectively. those complexes work as a h(+) and a na(+) channel, respectively and play important roles in torque generation as the stators of the flagellar motors. although asp32 of motb and asp24 of pomb are believed to function as ion binding site(s), the ion flux pathway from the periplasm to the cytoplasm is still unclear. conserved residues, ala39 of motb and cys31 of p ...200927857578
adherence assays and slime production of vibrio alginolyticus and vibrio parahaemolyticus.in this study we investigated the phenotypic slime production of vibrio alginolyticus and vibrio parahaemolyticus strains, food-borne pathogens, using a congo red agar plate assay. furthermore, we studied their ability to adhere to abiotic surfaces and vero cells line. our results showed that only v. alginolyticus atcc 17749 was a slime-producer developing almost black colonies on congo red agar plate. adherence to glace tube showed that all v. alginolyticus strains were more adherent than v. pa ...200924031378
[treatment of bacillary dysentery with wintomylon]. 20094962909
solitary congenital nodular calcification of winer located on the ear: report of two cases.solitary congenital nodular calcification of the skin is a form of cutaneous calcinosis. it appears as a small, firm nodule on the head and extremities of young infants. the histogenesis of this condition is controversial. we cared for two patients with the nodule on the ear.20092798256
solitary congenital nodular calcification of winer located on the ear: report of two cases.solitary congenital nodular calcification of the skin is a form of cutaneous calcinosis. it appears as a small, firm nodule on the head and extremities of young infants. the histogenesis of this condition is controversial. we cared for two patients with the nodule on the ear.20092798256
anomalous origin of right pulmonary artery from the ascending aorta.anomalous origin of one pulmonary artery from the ascending aorta without associated intracardiac defects is a rare congenital malformation. about 60 anatomic and surgical descriptions have been reported in the literature. up to 1974, 22 of the 50 reported cases underwent surgical correction with a 40% mortality rate. the natural history without surgery is poor with a very high mortality during the first year of life. successful anatomic correction of anomalous origin of right pulmonary artery f ...20092777879
cetus banks on interleukin. 20092787891
[reservoirs of resistance plasmids]. 20093327947
[children and domestic fights. a review of 105 social reports].the object of this investigation was to describe the conditions for children in families with domestic rows on the basis of 105 social reports made by the copenhagen police during a period of one year. a total of 162 children were involved in domestic rows. the typical family had 1-2 children. in 14% of the cases, the domestic rows were reported by the child. in connection with the domestic rows, violence to the women occurred in just under half of the cases and to the children in 6%. in 77% of ...20092711507
[methods of capsular and intracapsular microsurgery, intracapsular implantation and sutureless fixation of artificial lenses].the author recommends mastering the methods of capsular and intracapsular microsurgery for cataracts on cadaver eyes. he advises to remove the cornea and the iris to have a better view of the technique. as the physician masters the technique, he screens the lens sac equatorial area by keeping the iris and later the cornea (to make the conditions as close to clinical surgery as possible) and then removes the iris and the cornea for controlling the quality of the capsular pocket preparation. artif ...20092718300
success of etched-metal bonded retainers with nonrigid connections: a clinical study.the use of etched-metal bonded retainers in combination with conventional fixed retainers has been described in the literature. this design creates several potential problems that can be overcome by the use of an etched-metal bonded retainer with a nonrigid connector as described in this article. also presented are the preliminary results of a clinical study of 25 restorations that had been in place 13 to 45 months (average 22 months). the preliminary results are encouraging. no failures have be ...20092681695
listening rate and comprehension as a function of preference for and exposure to time-altered speech.we conducted experiments on the effects of brief prior exposure to time-altered speech on preferred listening rate and the rate listeners would select when asked to listen to speech as fast as possible with good compression (induced listening rate). in exp. 1, 48 participants were exposed either to normal rate speech or to speech compressed to twice-normal rate. brief exposure to twice-normal rate speech led to a faster induced listening rate than exposure to normal rate speech. in exp. 2, 31 pa ...20092717361
the immune response to intraparenchymal fetal cns transplants. 20092650446
[intraoperative monitoring of cerebral function and carotid hemodynamics during carotid endarterectomy].the authors investigated the hemodynamics and monitored the cerebral function to perform the carotid endarterectomy (cea) safely. the hemodynamics were investigated by measurements of carotid arterial blood flow by an electromagnetic flow meter before and after cea. and a doppler flow meter applied directly to the carotid arteries to analyze the flow parameters such as peak frequencies (pf), mean frequencies (mf), mode frequencies (mo f) and % window. we used routinely our specially designed shu ...20092812257
[intraoperative monitoring of cerebral function and carotid hemodynamics during carotid endarterectomy].the authors investigated the hemodynamics and monitored the cerebral function to perform the carotid endarterectomy (cea) safely. the hemodynamics were investigated by measurements of carotid arterial blood flow by an electromagnetic flow meter before and after cea. and a doppler flow meter applied directly to the carotid arteries to analyze the flow parameters such as peak frequencies (pf), mean frequencies (mf), mode frequencies (mo f) and % window. we used routinely our specially designed shu ...20092812257
malignant schwannoma. 20092724751
[necrosis of the bone marrow].the authors analyzed the incidence of necrosis of the bone marrow in nonselected autopsy material. the studies included 419 cases of various disease types. necrosis of the bone marrow was found in 83 cases (19.8%). it was most frequently observed in patients with neoplastic disease of the hematopoietic and lymphatic systems (26.3%) and malignant neoplasms of different origin (21.9%). in 40% cases necrosis of the bone marrow were accompanied by neoplastic metastases into the hematopoietic tissue, ...20092637982
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