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the effects of long-term occupational exposure to dust from herbs.herbs are a heterogeneous group of many species with several thousand plants, which are used in large quantities in the pharmaceutical and food industries. the aim of the study was to analyse the health effects of long-term occupational exposure to dust from herbs.200616217676
effects of yeasts and bacteria on the levels of folates in rye sourdoughs.fermentation of rye dough is often accompanied with an increase in folate content. in this study, three sourdough yeasts, candida milleri cbs 8195, saccharomyces cerevisiae ts 146, and torulaspora delbrueckii ts 207; a control, baker's yeast s. cerevisiae alko 743; and four lactobacillus spp., l. acidophilus tsb 262, l. brevis tsb 307, l. plantarum tsb 304, and l. sanfranciscensis tsb 299 originally isolated from rye sourdough were examined for their abilities to produce or consume folates. the ...200616213050
specific messenger rna expression for signal transduction molecules by lipopolysaccharide in intestinal macrophages.intestinal macrophages are known to display profound inflammatory anergy in response to lipopolysacchraide (lps). to study the mechanisms of unresponsiveness of intestinal macrophages to lps, we compared the mrna expression of molecules associated with signal transduction of intestinal macrophages with those of other tissue macrophages. also cellular localization of cd14 protein was examined. intestinal, alveolar and peritoneal macrophages were isolated from rats or mice. the expression of mrna ...200616487248
the efficacy of electrolysed oxidising water for inactivating spoilage microorganisms in process water and on minimally processed vegetables.the efficacy of electrolysed oxidising water (eow) for inactivating spoilage microorganisms in process water and on minimally processed vegetables was investigated. the direct effect of eow on three important spoilage bacteria namely; pseudomonas fluorescens, pantoea agglomerans or rahnella aquatilis was determined by inoculating tap water or "artificial process water" with approximately 8 log cfu/ml pure culture and electrolysing the resultant solutions. the three bacteria were each reduced to ...200616504328
pantoea agglomerans sepsis after rotavirus gastroenteritis. 200616511401
the phytopathogen dickeya dadantii (erwinia chrysanthemi 3937) is a pathogen of the pea aphid.dickeya dadantii (erwinia chrysanthemi) is a phytopathogenic bacterium causing soft rot diseases on many crops. the sequencing of its genome identified four genes encoding homologues of the cyt family of insecticidal toxins from bacillus thuringiensis, which are not present in the close relative pectobacterium carotovorum subsp. atrosepticum. the pathogenicity of d. dadantii was tested on the pea aphid acyrthosiphon pisum, and the bacterium was shown to be highly virulent for this insect, either ...200616517643
evaluation of the phoenix 100 id/ast system and nid panel for identification of enterobacteriaceae, vibrionaceae, and commonly isolated nonenteric gram-negative bacilli.the phoenix 100 id/ast system (becton dickinson co., sparks, md.) is an automated system for the identification and antimicrobial susceptibility testing of bacterial isolates. this system with its negative identification (nid) panel was evaluated for its accuracy in the identification of 507 isolates of the family enterobacteriaceae, 57 other nonenteric gram-negative isolates that are commonly isolated in clinical microbiology laboratories, and 138 isolates of the family vibrionaceae. all of the ...200616517878
indoleacetic acid, a product of transferred dna, inhibits vir gene expression and growth of agrobacterium tumefaciens c58.agrobacterium tumefaciens induces crown gall tumors by transferring a piece of its tumor-inducing plasmid into plant cells. this transferred dna encodes the synthesis of indole acetic acid (iaa) and cytokinin, and their overproduction results in tumor formation. the transfer is initiated by a two-component regulatory system, vira/g recognizing plant signal molecules in the plant rhizosphere and activating a regulon on the tumor-inducing plasmid, which is required for the processing and transfer ...200616537403
using bacteriophages to reduce formation of catheter-associated biofilms by staphylococcus epidermidis.use of indwelling catheters is often compromised as a result of biofilm formation. this study investigated if hydrogel-coated catheters pretreated with a coagulase-negative bacteriophage would reduce staphylococcus epidermidis biofilm formation. biofilms were developed on hydrogel-coated silicone catheters installed in a modified drip flow reactor. catheter segments were pretreated with the lytic s. epidermidis bacteriophage 456 by exposing the catheter lumen to a 10-log-pfu/ml culture of the ba ...200616569839
comparative anatomy of gall development on gypsophila paniculata induced by bacteria with different mechanisms of pathogenicity.galls induced on gypsophila paniculata by pantoea agglomerans pv. gypsophilae (pag) and agrobacterium tumefaciens (at), bacteria with different mechanisms of pathogenicity, were compared morphologically and anatomically. the pathogenicity of pag is dependent on the presence of an indigenous plasmid that harbors hrp gene cluster, genes encoding hop virulence proteins and biosynthetic genes for auxin (iaa) and cytokinins (cks), whereas that of at involves host transformation. the pag-induced gall ...200616477460
volatile metabolite production of spoilage micro-organisms on a mixed-lettuce agar during storage at 7 degrees c in air and low oxygen atmosphere.this paper describes the volatile metabolite production of spoilage bacteria (pantoea agglomerans and rahnella aquatilis) and spoilage yeasts (pichia fermentans and cryptococcus laurentii), previously isolated from mixed lettuce, on a simulation medium of shredded mixed lettuce (mixed-lettuce agar) both under air conditions and modified atmosphere (ma)-conditions at 7 degrees c. these latter conditions simulated the equilibrium modified atmosphere packaging, which is used to extend the shelf-lif ...200616860421
exopolysaccharides of pantoea agglomerans have different priming and eliciting activities in suspension-cultured cells of monocots and dicots.induced disease resistance of plants is often associated with an enhanced capacity to activate cellular defense responses to pathogen attack, named the "primed" state of the plant. exopolysaccharides of pantoea agglomerans have recently been reported as the first priming active component of bacterial origin in wheat cells. we now show that pantoea exopolysaccharides also prime rice cells for better elicitation of a rapid oxidative burst. in contrast, in tobacco and parsley cell cultures pantoea ...200616860795
antimicrobial activity of vanillin against spoilage microorganisms in stored fresh-cut mangoes.the antimicrobial activity of vanillin against four bacteria (pantoea agglomerans, aeromonas enteropelogenes, micrococcus lylae, and sphingobacterium spiritovorun), four fungi (alternaria sp., aspergillus sp., penicillium sp., and fusarium sp.), and three unidentified yeasts isolated from spoiling fresh-cut mango slices was verified in laboratory media adjusted to ph 5.0. mics of vanillin against the fungi (12.5 to 13.3 mm), bacteria (10 to 13.3 mm), and yeasts (5.0 to 6.7 mm) indicated that all ...200616865911
the type iii effectors hsvg and hsvb of gall-forming pantoea agglomerans determine host specificity and function as transcriptional activators.pantoea agglomerans pv. gypsophilae (pag) elicits galls on gypsophila and a hypersensitive response on beet, whereas p. agglomerans pv. betae (pab) induces galls on both beet and gypsophila. the pathogenicity of both pathovars is dependent on the presence of a plasmid harbouring type iii secretion system (ttss) components and effectors. the hsvg ttss effectors of pag (hsvg-pag) and pab (hsvg-pab) determine the host specificity of both pathovars on gypsophila. here we describe a novel hsvg homolo ...200616879413
renal infarction and immune-mediated glomerulonephritis in sheep (ovis aries) chronically implanted with indwelling catheters.microbial infections are common sequelae in humans and animals implanted with long-term intravascular catheters. understanding the pathophysiology of infectious morbidity is critical to improving quality of care in catheterized subjects. here, we describe findings in 6 clinically healthy, male sheep implanted with indwelling aortic or cardiac catheters for 6 to 10 mo. we isolated multiple bacterial species including serratia spp., enterobacter agglomerans, eschericia coli, klebsiella oxytoca, an ...200616884173
peptidoglycan from bacillus cereus mediates commensalism with rhizosphere bacteria from the cytophaga-flavobacterium group.previous research in our laboratory revealed that the introduction of bacillus cereus uw85 can increase the populations of bacteria from the cytophaga-flavobacterium (cf) group of the bacteroidetes phylum in the soybean rhizosphere, suggesting that these rhizosphere microorganisms have a beneficial relationship (g. s. gilbert, j. l. parke, m. k. clayton, and j. handelsman, ecology 74:840-854, 1993). in the present study, we determined the frequency at which cf bacteria coisolated with b. cereus ...200616885294
two transsulfurylation pathways in klebsiella pneumoniae.in most bacteria, inorganic sulfur is assimilated into cysteine, which provides sulfur for methionine biosynthesis via transsulfurylation. here, cysteine is transferred to the terminal carbon of homoserine via its sulfhydryl group to form cystathionine, which is cleaved to yield homocysteine. in the enteric bacteria escherichia coli and salmonella enterica, these reactions are catalyzed by irreversible cystathionine-gamma-synthase and cystathionine-beta-lyase enzymes. alternatively, yeast and so ...200616885444
analysis of ests from lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship.an expressed sequence tag library has been generated from a sand fly vector of visceral leishmaniasis, lutzomyia longipalpis. a normalized cdna library was constructed from whole adults and 16,608 clones were sequenced from both ends and assembled into 10,203 contigs and singlets. of these 58% showed significant similarity to known genes from other organisms, <4% were identical to described sand fly genes, and 42% had no match to any database sequence. our analyses revealed putative proteins inv ...200616887324
a biosynthetic gene cluster for the acetyl-coa carboxylase inhibitor andrimid.increasing bacterial resistance to antibiotics with conventional targets has focused attention on antibiotics with unconventional targets. one promising candidate, the acetyl-coa carboxylase (acc) inhibitor andrimid, is a potent, broad-spectrum antibiotic with high selectivity for prokaryotic acc. here, we report the use of a dna-based approach to clone the andrimid biosynthetic gene cluster from pantoea agglomerans, yielding a cosmid that confers robust andrimid production on escherichia coli. ...200616910643
[bacteremia caused by pantoea agglomerans]. 200616913075
aetiology, risk factors and immediate outcome of bacteriologically confirmed neonatal septicaemia in mulago hospital, uganda.neonatal septicaemia remains a major cause of morbidity and mortality. the aetiology, risk factors and outcome of this problem need to understood.200616916305
two genes encoding new carotenoid-modifying enzymes in the green sulfur bacterium chlorobium tepidum.the green sulfur bacterium chlorobium tepidum produces chlorobactene as its primary carotenoid. small amounts of chlorobactene are hydroxylated by the enzyme crtc and then glucosylated and acylated to produce chlorobactene glucoside laurate. the genes encoding the enzymes responsible for these modifications of chlorobactene, ct1987, and ct0967, have been identified by comparative genomics, and these genes were insertionally inactivated in c. tepidum to verify their predicted function. the gene e ...200616923888
integrated regulation of the type iii secretion system and other virulence determinants in ralstonia solanacearum.in many plant and animal bacterial pathogens, the type iii secretion system (ttss) that directly translocates effector proteins into the eukaryotic host cells is essential for the development of disease. in all species studied, the transcription of the ttss and most of its effector substrates is tightly regulated by a succession of consecutively activated regulators. however, the whole genetic programme driven by these regulatory cascades is still unknown, especially in bacterial plant pathogens ...200616933989
mutational and functional analysis of the beta-carotene ketolase involved in the production of canthaxanthin and astaxanthin.biosynthesis of the commercial carotenoids canthaxanthin and astaxanthin requires beta-carotene ketolase. the functional importance of the conserved amino acid residues of this enzyme from paracoccus sp. strain n81106 (formerly classified as agrobacterium aurantiacum) was analyzed by alanine-scanning mutagenesis. mutations in the three highly conserved histidine motifs involved in iron coordination abolished its ability to catalyze the formation of ketocarotenoids. this supports the hypothesis t ...200616957201
identification, typing, and insecticidal activity of xenorhabdus isolates from entomopathogenic nematodes in united kingdom soil and characterization of the xpt toxin loci.xenorhabdus strains from entomopathogenic nematodes isolated from united kingdom soils by using the insect bait entrapment method were characterized by partial sequencing of the 16s rrna gene, four housekeeping genes (asd, ompr, reca, and serc) and the flagellin gene (flic). most strains (191/197) were found to have genes with greatest similarity to those of xenorhabdus bovienii, and the remaining six strains had genes most similar to those of xenorhabdus nematophila. generally, 16s rrna sequenc ...200616957209
invasive enterobacter sakazakii disease in infants.enterobacter sakazakii kills 40%-80% of infected infants and has been associated with powdered formula. we analyzed 46 cases of invasive infant e. sakazakii infection to define risk factors and guide prevention and treatment. twelve infants had bacteremia, 33 had meningitis, and 1 had a urinary tract infection. compared with infants with isolated bacteremia, infants with meningitis had greater birthweight (2,454 g vs. 850 g, p = 0.002) and gestational age (37 weeks vs. 27.8 weeks, p = 0.02), and ...200616965695
improving low water activity and desiccation tolerance of the biocontrol agent pantoea agglomerans cpa-2 by osmotic treatments.to study the improvement of tolerance to low water activity (aw) and desiccation during spray drying in pantoea agglomerans cells subjected to mild osmotic stress during growth.200616968304
identification of a novel regulatory protein (csrd) that targets the global regulatory rnas csrb and csrc for degradation by rnase e.in escherichia coli, the global regulatory protein csra (carbon store regulator a) binds to leader segments of target mrnas, affecting their translation and stability. csra activity is regulated by two noncoding rnas, csrb and csrc, which act by sequestering multiple csra dimers. here, we describe a protein (csrd) that controls the degradation of csrb/c rnas. the dramatic stabilization of csrb/c rnas in a csrd mutant altered the expression of csra-controlled genes in a manner predicted from the ...200616980588
ralstonia solanacearum requires f-box-like domain-containing type iii effectors to promote disease on several host plants.the phytopathogenic bacterium ralstonia solanacearum encodes a family of seven type iii secretion system (t3ss) effectors that contain both a leucine-rich repeat and an f-box domain. this structure is reminiscent of a class of typical eukaryotic proteins called f-box proteins. the latter, together with skp1 and cullin1 subunits, constitute the scf-type e3 ubiquitin ligase complex and control specific protein ubiquitinylation. in the eukaryotic cell, depending on the nature of the polyubiquitin c ...200616983093
disruption of n-acyl homoserine lactone-mediated cell signaling and iron acquisition in epiphytic bacteria by leaf surface compounds.since n-acyl homoserine lactones (ahls) are key mediators of cell density-dependent regulation of traits involved in virulence and epiphytic fitness in gram-negative bacteria such as pseudomonas syringae, a variety of plant species were examined to determine their production of leaf surface compounds that could interact with these signaling systems. leaf washings of 17 of 52 plant species tested stimulated or inhibited ahl-dependent traits in at least one of the bacterial reporter strains used. ...200616997987
two-center collaborative evaluation of performance of the bd phoenix automated microbiology system for identification and antimicrobial susceptibility testing of gram-negative bacteria.the performance of the bd phoenix automated microbiology system (bd diagnostic systems, sparks, md) was assessed for identification (id) and antimicrobial susceptibility testing (ast) of the majority of clinically encountered bacterial isolates in a european collaborative two-center trial. a total of 494 bacterial isolates including various species of the enterobacteriaceae and 110 nonfermentative gram-negative bacteria were investigated: of these, 385 were single patient isolates, and 109 were ...200617005752
evaluation of the bd phoenix automated microbiology system for identification and antimicrobial susceptibility testing of enterobacteriaceae.we evaluated the accuracy of the bd phoenix system for the identification (id) and antimicrobial susceptibility testing (ast) of 251 isolates of the family enterobacteriaceae representing 31 species. organisms were inoculated onto the phoenix panel according to the manufacturer's instructions. the results from conventional biochemical tests were used for the reference method for id. agar dilution, performed according to the clsi guidelines, was the reference ast method. essential and categorical ...200617021074
identification of a putative operon involved in fructooligosaccharide utilization by lactobacillus paracasei.the growth and activity of some lactobacillus and bifidobacterium strains are stimulated by the presence of nondigestible fructooligosaccharides (fos), which are selectively fermented by specific intestinal bacteria. consumption of fos, therefore, enriches for those bacteria that possess metabolic pathways necessary for fos metabolism. in this study, a dna microarray consisting of 7,680 random genomic library fragments of lactobacillus paracasei 1195 was used to examine genes involved in the uti ...200617028235
evaluation of the 10th external quality assessment scheme results in clinical microbiology laboratories in tehran and districts.we evaluated the performance of microbiology laboratories in the 10th run of the external quality assessment scheme (eqas) in tehran and districts. each laboratory was sent 2 species of bacteria for identification. of the 487 laboratories that participated, 437 returned their findings. while 77.0% and 69.9% correctly identified staphylococcus saprophyticus and citrobacter freundii respectively, only 29.8% correctly identified acinetobacter baumanii, 25.3% identified enterococcus faecalis and 35. ...200617037699
preliminary crystallographic characterization of prnb, the second enzyme in the pyrrolnitrin biosynthetic pathway.pyrrolnitrin is the active ingredient of drugs for the treatment of superficial fungal infections and was used as a lead structure for the development of fludioxonil. it is an effective agent for plant diseases caused by the fungal pathogen rhizoctonia solani. pyrrolnitrin is made in four steps, the second of which, catalyzed by prnb, is a novel chemical rearrangement of 7-chlorotryptophan. prnb was overproduced in pseudomonas fluorescens (bl915) and well diffracting crystals were obtained of a ...200617077497
responses of the anaerobic bacterial community to addition of organic c in chromium(vi)- and iron(iii)-amended microcosms.chromium (vi) is toxic to microorganisms and can inhibit the biodegradation of organic pollutants in contaminated soils. we used microcosms amended with either glucose or protein (to drive bacterial community change) and fe(iii) (to stimulate iron-reducing bacteria) to study the effect of various concentrations of cr(vi) on anaerobic bacterial communities. microcosms were destructively sampled based on microbial activity (measured as evolution of co2) and analyzed for the following: (i) dominant ...200616391100
abundant and diverse fungal microbiota in the murine intestine.enteric microbiota play a variety of roles in intestinal health and disease. while bacteria in the intestine have been broadly characterized, little is known about the abundance or diversity of enteric fungi. this study utilized a culture-independent method termed oligonucleotide fingerprinting of rrna genes (ofrg) to describe the compositions of fungal and bacterial rrna genes from small and large intestines (tissue and luminal contents) of restricted-flora and specific-pathogen-free mice. ofrg ...200616391120
the genus hafnia: from soup to nuts.the genus hafnia, a member of the family enterobacteriaceae, consists of gram-negative bacteria that are occasionally implicated in both intestinal and extraintestinal infections in humans. despite the fact that the genus currently contains only a single species (h. alvei), more extensive phylogenetic depth (two or more species) is apparent based upon dna relatedness and 16s rrna gene sequencing studies. hafnia causes a variety of systemic infections, including septicemia and pneumonia; however, ...200616418520
evaluation of antimicrobial activity of the stem bark of cylicodiscus gabunensis (mimosaceae).ethyl acetate (ea) extract of the stem bark of cylicodiscus gabunensis (cg) was analysed phytochemically and evaluated for its antimicrobial activity against 17 pathogenic species isolated from patient: escherichia coli, klebsiella pneumoniae, shigella dysenteriae, shigella flexneri, morganella morganii, proteus vulgaris, proteus mirabilis, salmonella typhi, citrobacter freundii, enterobacter cloacae, enterobacter agglomerans, staphylococcus aureus, streptococcus feacalis, pseudomonas aeruginosa ...200620162076
applications of lipopolysaccharide derived from pantoea agglomerans (ip-pa1) for health care based on macrophage network theory.innate immunity is a universal prophylactic system which all multi-cellular animals possess. macrophages are the cells that play the central role in the innate immune system. in 1991, we discovered a substance in a water extract of wheat flour that activated macrophages after oral or intradermal administration. the active substance was lipopolysaccharide (lps), which is derived from the cell walls of pantoea agglomerans, gram-negative bacteria that grows symbiotically with wheat. we named the su ...200617270712
antifungal and antibacterial activities of mexican tarragon (tagetes lucida).mexican tarragon (tagetes lucida cv. asteraceae: campanulatae) is an important, nutritious plant and an effective herbal medicine. seven coumarins, 7,8-dihydroxycoumarin (4), umbelliferone (7-hydroxycoumarin) (5), scoparone (6,7-dimethoxycoumarin) (7), esculetin (6,7-dihydroxycoumarin) (11), 6-hydroxy-7-methoxycoumarin (12), herniarin (7-methoxycoumarin) (13), and scopoletin (6-methoxy-7-hydroxycoumarin) (14), and three flavonoids, patuletin (18), quercetin (19), and quercetagetin (20), were iso ...200619127719
spent growth medium of pantoea agglomerans primes wheat suspension cells for augmented accumulation of hydrogen peroxide and enhanced peroxidase activity upon elicitation.induced disease resistance in plants is based on multiple mechanisms, including cell "priming", i.e. an enhancement of the capacity to mobilize cellular defense responses upon pathogen attack. potent inducers of priming are, for example, salicylic acid, synthetic compounds such as a benzothiadiazole, and certain rhizosphere bacteria. while priming is well characterized for a number of dicot plants, only few cases of priming are documented in monocots. here, we report that the spent growth medium ...200616596409
assessment of the environmental fate of the biological control agent of fire blight, pseudomonas fluorescens eps62e, on apple by culture and real-time pcr methods.the colonization of apple blossoms and leaves by pseudomonas fluorescens eps62e was monitored in greenhouse and field trials using cultivable cell counting and real-time pcr. the real-time pcr provided a specific quantitative method for the detection of strain eps62e. the detection level was around 10(2) cells g (fresh weight)(-1) and the standard curve was linear within a 5-log range. eps62e actively colonized flowers reaching values from 10(7) to 10(8) cells per blossom. in apple flowers, no s ...200616597940
sll0254 (crtl(diox)) is a bifunctional lycopene cyclase/dioxygenase in cyanobacteria producing myxoxanthophyll.upon depletion of sll0254 in synechocystis sp. strain pcc 6803, cyclized carotenoids were replaced by linear, relatively hydrophilic carotenoids, and the amount of the two photosystems decreased greatly. full segregants of the sll0254 deletion in synechocystis were not obtained, implying that this gene is essential for survival, most likely to allow normal cell division. the n-terminal half of sll0254 has limited similarity to the family of lycopene cyclases, has an additional dehydrogenase moti ...200616621828
rice endophyte pantoea agglomerans ys19 promotes host plant growth and affects allocations of host photosynthates.the aims of the study were to identify the effects of rice endophyte pantoea agglomerans ys19 on host plant growth and allocations of photosynthates.200616629994
scalp abscess due to enterobacter agglomerans. 200616650188
the survival of pathogens in soil treated with wastewater sludge and in potatoes grown in such soil.the prevalence of pathogens on potatoes (solanum tuberosum) grown in soil amended with a pathogen rich wastewater sludge was investigated. bacteria of the family enterobacteriaceae are important pathogens causing intestinal and systemic illness of humans and other animals. type b sludge was used. sludges investigated are the high metal and the low metal sludges. microorganisms in the sludge-amended soil were using culture-based technique. salmonella and e. coli were observed in tested soil sampl ...200617087382
pantoea peritonitis in a patient receiving chronic ambulatory peritoneal dialysis.pantoea agglomerans is usually the most common organism transmitted through plant thorn injuries. this report is of a female patient maintained on chronic ambulatory peritoneal dialysis (capd) who developed peritonitis attributed to p. agglomerans. peritonitis is an uncommon complication of p. agglomerans and there is no previous report of peritonitis associated with this organism in a capd patient. the source of infection was thought to be due to rose-thorn injury. antibiotic therapy with cefta ...200616669968
characterization of a collection of enterobacter sakazakii isolates from environmental and food sources.enterobacter sakazakii has emerged as a rare cause of neonatal meningitis, septicemia and enterocolitis. contaminated infant milk formula (imf) has been identified as one infection route. a small number of clinical outbreaks have been epidemiologically linked to imf contaminated post-pasteurization during manufacture and/or mishandled when reconstituted. currently no agreed standardized typing protocol has been developed to trace e. sakazakii. the objectives of this study were to apply biochemic ...200616730386
characterization of fe(iii)-reducing enrichment cultures and isolation of fe(iii)-reducing bacteria from the savannah river site, south carolina.the savannah river site, south carolina (srs), has been subjected to heavy metal and radionuclide contamination. dissimilatory fe(iii)-reducing bacteria, by reducing insoluble fe(iii) to soluble fe(ii), may enhance contaminant mobility through subsurface environments. in order to investigate populations of the indigenous iron-reducing microbes from the srs, duplicate enrichment cultures were initiated using a 10% inoculum of 7 sediment/soil samples, and serial dilutions were made into wolfe's mi ...200616730954
biochemical characterization of prephenate dehydrogenase from the hyperthermophilic bacterium aquifex aeolicus.a monofunctional prephenate dehydrogenase (pd) from aquifex aeolicus was expressed as a his-tagged protein in escherichia coli and was purified by nickel affinity chromatography allowing the first biochemical and biophysical characterization of a thermostable pd. a. aeolicus pd is susceptible to proteolysis. in this report, the properties of the full-length pd are compared with one of these products, an n-terminally truncated protein variant (delta19pd) also expressed recombinantly in e. coli. b ...200616731976
enhanced biofilm formation and increased resistance to antimicrobial agents and bacterial invasion are caused by synergistic interactions in multispecies biofilms.most biofilms in their natural environments are likely to consist of consortia of species that influence each other in synergistic and antagonistic manners. however, few reports specifically address interactions within multispecies biofilms. in this study, 17 epiphytic bacterial strains, isolated from the surface of the marine alga ulva australis, were screened for synergistic interactions within biofilms when present together in different combinations. four isolates, microbacterium phyllosphaer ...200616751497
a constitutively expressed, truncated umudc operon regulates the reca-dependent dna damage induction of a gene in acinetobacter baylyi strain adp1.in response to environmentally caused dna damage, sos genes are up-regulated due to reca-mediated relief of lexa repression. in escherichia coli, the sos umudc operon is required for dna damage checkpoint functions and for replicating damaged dna in the error-prone process called sos mutagenesis. in the model soil bacterium acinetobacter baylyi strain adp1, however, the content, regulation, and function of the umudc operon are unusual. the umuc gene is incomplete, and a remnant of an isehe3-like ...200616751513
structures of apo- and holo-tyrosine phenol-lyase reveal a catalytically critical closed conformation and suggest a mechanism for activation by k+ ions.tyrosine phenol-lyase, a tetrameric pyridoxal 5'-phosphate dependent enzyme, catalyzes the reversible hydrolytic cleavage of l-tyrosine to phenol and ammonium pyruvate. here we describe the crystal structure of the citrobacter freundii holoenzyme at 1.9 a resolution. the structure reveals a network of protein interactions with the cofactor, pyridoxal 5'-phosphate, and details of coordination of the catalytically important k+ ion. we also present the structure of the apoenzyme at 1.85 a resolutio ...200616768450
quinine sulfate inhibits invasion of some bacterial skin pathogens.as some of the many patients who receive antimalarials for the treatment of noninfective inflammatory diseases (lupus erythematosus, collagen vascular diseases, rheumatoid arthritis, and others) are also immunosuppressed because of their disease and/or treatments, and may have concomitant bacterial infections, we investigated the effect of these drugs on the growth and invasion of several bacteria that are commonly associated with skin and soft tissue infections to determine whether they could p ...200616796622
antimicrobial activity and spectrum of cefovecin, a new extended- spectrum cephalosporin, against pathogens collected from dogs and cats in europe and north america.cefovecin is a new extended-spectrum semisynthetic cephalosporin indicated for the treatment of bacterial infections in dogs and cats. this study evaluated the in vitro activity and spectrum of cefovecin against 2,641 recent clinical isolates (1,660 canine and 981 feline isolates) from europe and the united states. mic determinations against cefovecin and other reference antimicrobials were performed by broth microdilution methods recommended by the clinical and laboratory standards institute (c ...200616801403
molecular characterization of pantoea stewartii subsp. stewartii hrpy, a conserved response regulator of the hrp type iii secretion system, and its interaction with the hrps promoter.pantoea stewartii subsp. stewartii is a bacterial pathogen of corn. its pathogenicity depends on the translocation of effector proteins into host cells by the hrp type iii secretion system. we previously showed by genetic analysis that the hrpx sensor kinase and the hrpy response regulator are at the head of a complex cascade of regulators controlling hrp/hrc secretion and wts effector genes. this cascade also includes the hrps response regulator and the hrpl alternative sigma factor. these regu ...200616816181
changes in the spoilage-related microbiota of beef during refrigerated storage under different packaging conditions.the microbial spoilage of beef was monitored during storage at 5 degrees c under three different conditions of modified-atmosphere packaging (map): (i) air (map1), (ii) 60% o2 and 40% co2 (map2), and (iii) 20% o2 and 40% co2 (map3). pseudomonas, enterobacteriaceae, brochothrix thermosphacta, and lactic acid bacteria were monitored by viable counts and pcr-denaturing gradient gel electrophoresis (dgge) analysis during 14 days of storage. moreover, headspace gas composition, weight loss, and beef ...200616820458
prediction of csra-regulating small rnas in bacteria and their experimental verification in vibrio fischeri.the role of small rnas as critical components of global regulatory networks has been highlighted by several recent studies. an important class of such small rnas is represented by csrb and csrc of escherichia coli, which control the activity of the global regulator csra. given the critical role played by csra in several bacterial species, an important problem is the identification of csra-regulating small rnas. in this paper, we develop a computer program (csrna_find) designed to locate potentia ...200616822857
aerobic bacterial flora of nesting green turtles (chelonia mydas) from tortuguero national park, costa rica.bacteriological examination of 70 nesting green turtles (chelonia mydas) from tortuguero national park, costa rica was performed to investigate nasal and cloacal aerobic bacteria. a total of 325 bacterial isolates were obtained, including 10 gram-negative and three gram-positive genera. two hundred thirty-nine were gram-negative and 86 were gram-positive isolates. klebsiella pneumoniae was the most common microbe identified in turtle samples: 27/70 (38.5%) in cloacal, and 33/70 (47.1%) in nasal ...200617315444
a preliminary study on gram-negative bacteria (gnb) and their endotoxins in a gin house in india.the presence of byssinosis, an occupational disease found among cotton mill workers, has been well documented in different parts of the world. the disease develops due to exposure to environmental cotton dust. evidence suggests that the causative agent for the disease is gram-negative bacteria (gnb) and their endotoxins present on the cotton fibers. an investigation was carried out in a gin house in western india. environmental dust samples were collected by vertical elutriator (ve). airborne du ...200617133691
biochemical and structural characterization of the secreted chorismate mutase (rv1885c) from mycobacterium tuberculosis h37rv: an *aroq enzyme not regulated by the aromatic amino acids.the gene rv1885c from the genome of mycobacterium tuberculosis h37rv encodes a monofunctional and secreted chorismate mutase (*mtcm) with a 33-amino-acid cleavable signal sequence; hence, it belongs to the *aroq class of chorismate mutases. consistent with the heterologously expressed *mtcm having periplasmic destination in escherichia coli and the absence of a discrete periplasmic compartment in m. tuberculosis, we show here that *mtcm secretes into the culture filtrate of m. tuberculosis. *mtc ...200617146044
a robotic dna purification protocol and real-time pcr for the detection of enterobacter sakazakii in powdered infant formulae.enterobacter sakazakii is the causative agent of rare but severe food-borne infections associated with meningitis, necrotizing enterocolitis and sepsis in infants. rehydrated powdered infant formulae have been implicated as the source of infection in several outbreaks and sporadic cases. in this work, a real time fluorescence resonance energy transfer pcr assay incorporating an internal amplification control (iac) was developed for the specific detection of e. sakazakii in foods. performance of ...200617166252
legionella and other gram-negative bacteria in potable water from various rural and urban sources.a total of 107 potable water samples were collected from various rural and urban sources located in the lublin region (eastern poland). 54 samples from rural sources comprised 32 samples of untreated well water and 22 samples of treated (chlorinated) tap water from rural dwellings distributed by the municipal water supply system (mwss). 53 samples of treated water from urban sources were supplied by the city of lublin mwss. they comprised: 11 samples of tap water from offices and shops, 8 sample ...200617196009
characterization of the bacterial microflora of the tympanic cavity of eastern box turtles with and without aural abscesses.aerobic bacterial cultures of the tympanic cavity of the middle ear were performed in eight eastern box turtles (terrapene carolina carolina) with aural abscesses and 15 eastern box turtles without aural abscesses (controls) that were admitted to the wildlife center of virginia, virginia, usa during 2003. twenty-two bacterial isolates were identified from 17 turtles including 10 gram-negative and 12 gram-positive bacteria. ten of 15 control animals had bacterial growth, resulting in identificati ...200617255456
monitoring bacterial communities in raw milk and cheese by culture-dependent and -independent 16s rrna gene-based analyses.the diversity and dynamics of bacterial populations in saint-nectaire, a raw-milk, semihard cheese, were investigated using a dual culture-dependent and direct molecular approach combining single-strand conformation polymorphism (sscp) fingerprinting and sequencing of 16s rrna genes. the dominant clones, among 125 16s rrna genes isolated from milk, belonged to members of the firmicutes (58% of the total clones) affiliated mainly with the orders clostridiales and the lactobacillales, followed by ...200717259356
a comparison of enzymatic phosphorylation and phosphatidylation of beta-l- and beta-d-nucleosides.enzymatic 5'-monophosphorylation and 5'-phosphatidylation of a number of beta-l- and beta-d-nucleosides was investigated. the first reaction, catalyzed by nucleoside phosphotransferase (npt) from erwinia herbicola, consisted of the transfer of the phosphate residue from p-nitrophenylphosphate (p-npp) to the 5'-hydroxyl group of nucleoside; the second was the phospholipase d (pld)-catalyzed transphosphatidylation of l-alpha-lecithin with a series of beta-l- and beta-d-nucleosides as the phosphati ...200717206374
role of pantoea agglomerans in opportunistic bacterial seed and boll rot of cotton (gossypium hirsutum) grown in the field.to investigate the aetiology of seed and boll rot of cotton grown in south carolina (sc).200717184328
investigations into the in vitro antimicrobial activity and mode of action of the phenazine antibiotic d-alanylgriseoluteic acid.d-alanylgriseoluteic acid (aga) is a potent antimicrobial phenazine compound produced by pantoea agglomerans (erwinia herbicola) eh1087. susceptibility tests against a range of microbes indicated that aga had a broad spectrum of antimicrobial activity and was particularly active against gram-positive pathogens. comparison of the in vitro efficacy of aga with eight other antibiotics against 119 clinical isolates of streptococcus pneumoniae demonstrated that all were inhibited by low concentration ...200717189100
lipopolysaccharide induces delayed fosb/deltafosb immunostaining within the mouse extended amygdala, hippocampus and hypothalamus, that parallel the expression of depressive-like behavior.proinflammatory cytokines induce both sickness behavior and depression, but their respective neurobiological correlates are still poorly understood. the aim of the present study was therefore to identify in mice the neural substrates of sickness and depressive-like behavior induced by lipopolysaccharide (lps, 830 microg/kg, intraperitoneal). lps-induced depressive-like behavior was dissociated from lps-induced sickness by testing mice either at 6 h (at which time sickness was expected to be maxi ...200717482371
characterization of the zinc-containing metalloprotease encoded by zpx and development of a species-specific detection method for enterobacter sakazakii.enterobacter sakazakii causes a severe form of neonatal meningitis that occurs as sporadic cases as well as outbreaks. the disease has been epidemiologically associated with consumption of reconstituted, dried infant formulas. very little information is available regarding pathogenicity of the organism and production of virulence factors. clinical and environmental strains were screened for production of factors which have activity against chinese hamster ovary (cho) cells in tissue culture. pol ...200717483271
increased beta-carotene production in recombinant escherichia coli harboring an engineered isoprenoid precursor pathway with mevalonate addition.when pt-lycm4 containing lycopene synthetic genes was co-transformed with psucrty or pshcrty containing crty gene of pantoea ananatis (p. ananatis) or pantoea agglomerans (p. agglomerans), beta-carotene productions of 36 and 35 mg/l were obtained, respectively. no lycopene was detected in the beta-carotene production culture. pt-hb, constructed by addition of p. ananatis crty gene into pt-lycm4, was used for co-transformation with psdxs and pssn12didi, which increased isopentenyl diphosphate and ...200717500531
characterization of the cultivable microbiota of sprouts and their potential for application as protective cultures.the microbiota of ten seeds and ready-to-eat sprouts produced thereof was characterized by bacteriological culture and denaturing gradient gel electrophoresis (dgge) of amplified dna fragments of the 16s rrna gene. the predominant bacterial biota of hydroponically grown sprouts mainly consisted of enterobacteria, pseudomonades and lactic acid bacteria (lab). for adzuki, alfalfa, mung bean, radish, sesame and wheat, the ratio of these bacterial groups changed strongly in the course of germination ...200717512686
[mechanism of the effect of lpsp on the production of anti-hbs in mice].to investigate the mechanism of the effect of avirulent pantoea agglomerans lipopolysaccharide (lpsp) as the adjuvant of hbsag on the production of anti-hbs in mice.200717553355
relative importance of amino acids, glycine-betaine and ectoine synthesis in the biocontrol agent pantoea agglomerans cpa-2 in response to osmotic, acidic and heat stress.the objective of this work was to determine the role of different compatible solutes in adaptation of pantoea agglomerans cpa-2 at different stages of growth to solute (0.98, 0.97, 0.96 aw), heat (35 and 40 degrees c) and acidic (ph 4.0, 5.0, 6.0) stress.200717594453
bacteremia caused by pantoea agglomerans and enterococcus faecalis in a patient with colon cancer.a case of bacteremia caused by pantoea agglomerans (p. agglomerans) and enterococcus faecalis in a male patient with colon cancer is reported. he was successfully treated with vancomycin and meropenem. the clinical isolate was identified as p. agglomerans by 16s rrna gene sequencing.200717600887
stability of microbial communities in goat milk during a lactation year: molecular approaches.the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ...200717604934
directed evolution can rapidly improve the activity of chimeric assembly-line enzymes.nonribosomal peptides (nrps) are produced by nrp synthetase (nrps) enzymes that function as molecular assembly lines. the modular architecture of nrpss suggests that a domain responsible for activating a building block could be replaced with a domain from a foreign nrps to create a chimeric assembly line that produces a new variant of a natural nrp. however, such chimeric nrps modules are often heavily impaired, impeding efforts to create novel nrp variants by swapping domains from different mod ...200717620609
atmospheric movement of microorganisms in clouds of desert dust and implications for human health.billions of tons of desert dust move through the atmosphere each year. the primary source regions, which include the sahara and sahel regions of north africa and the gobi and takla makan regions of asia, are capable of dispersing significant quantities of desert dust across the traditionally viewed oceanic barriers. while a considerable amount of research by scientists has addressed atmospheric pathways and aerosol chemistry, very few studies to determine the numbers and types of microorganisms ...200717630335
transmission of cotton seed and boll rotting bacteria by the southern green stink bug (nezara viridula l.).to determine the ability of the southern green stink bug (sgsb) (nezara viridula l.) to transmit pantoea agglomerans into cotton (gossypium hirsutum) bolls.200717650204
bilateral tibial chronic osteomyelitis due to pantoea agglomerans in a patient with sickle cell disease. 200717650525
incorporation of nonmethyl branches by isoprenoid-like logic: multiple beta-alkylation events in the biosynthesis of myxovirescin a1.several polyketide secondary metabolites are predicted to undergo isoprenoid-like beta-alkylations during biosynthesis. one such secondary metabolite is myxovirescin a1, produced by myxococcus xanthus. myxovirescin is of special interest in that it appears to undergo two distinct beta-alkylations. additionally, the myxovirescin biosynthetic gene cluster lacks tandem thiolation domains required in the synthesis of other beta-branched secondary metabolites. to probe the origins of the beta-branche ...200717656320
[effects of a lactic acid bacteria community sfc-2 treated on rice straw].aimed to utilize rice straw and lessen the pressure of environment, the rice straw was used as the fermentation material, and a lactic acid bacteria community sfc-2 from my laboratory was inoculated into the rice straw to investigate the inoculation effects. after 30 days fermentation, the inoculated fermented straw smelt acid-fragrant, and the ph value was 3.8, which was lower than the control of 4.1. furthermore, lactic acid concentration was more than that in the control. especially l-lactic ...200717674756
high performance microbiological transformation of l-tyrosine to l-dopa by yarrowia lipolytica nrrl-143.the 3,4-dihydroxy phenyl l-alanine (l-dopa) is a drug of choice for parkinson's disease, controlling changes in energy metabolism enzymes of the myocardium following neurogenic injury. aspergillus oryzae is commonly used for l-dopa production; however, potential improvements in ease of handling, growth rate and environmental impact have led to an interest in exploiting alternative yeasts. the two important elements required for l-dopa production are intracellular tyrosinases (thus pre-grown yeas ...200717705832
microbial degradation of phosmet on blueberry fruit and in aqueous systems by indigenous bacterial flora on lowbush blueberries (vaccinium angustifolium).phosmet-adapted bacteria isolated from lowbush blueberries (vaccinium angustifolium) were evaluated for their ability to degrade phosmet on blueberry fruit and in minimal salt solutions. microbial metabolism of phosmet by isolates of enterobacter agglomerans and pseudomonas fluorescens resulted in significant reductions (p < 0.05; 33.8%) in phosmet residues on blueberry fruit. degradation was accompanied by microbial proliferation of phosmet-adapted bacteria. preferential utilization of phosmet ...200717995608
virulence mechanisms and host specificity of gall-forming pantoea agglomerans.pantoea agglomerans has been transformed from a commensal bacterium associated with many plants into a host-specific gall-forming pathogen by acquiring a plasmid-borne pathogenicity island. this pathogenicity island harbors the hrp/hrc gene cluster, in addition to genes encoding type iii effector proteins, biosynthesis of the phytohormones indole-3-acetic acid and cytokinin, multiple diverse insertion sequences and pseudogenes. this review describes a unique model for understanding the emergence ...200718024130
a novel beta-galactosidase capable of glycosyl transfer from enterobacter agglomerans b1.a novel transglycosylating beta-galactosidase was purified from enterobacter agglomerans b1. it was a homodimer of approximately 248 kda. the optimal ph and temperature for onpgal hydrolysis were 7.5-8.0 and 37-40 degrees c, respectively. the k(m) values for onpgal and lactose were 0.06 and 114 mm, respectively. the enzyme produced galacto-oligosaccharides in a 38% yield at the lactose concentration of 12.5% (w/v). when using onpgal as donor, the enzyme was able to catalyze glycosyl transfer to ...200717336932
characterization of extended-host-range pseudo-t-even bacteriophage kpp95 isolated on klebsiella pneumoniae.kpp95, isolated on klebsiella pneumoniae, is a bacteriophage with the morphology of t4-type phages and is capable of rapid lysis of host cells. its double-stranded genomic dna (ca. 175 kb, estimated by pulsed-field gel electrophoresis) can be cut only by restriction endonucleases with a cleavage site flanked either by a and t or by t, as tested, suggesting that it contains the modified derivative(s) of g and/or c. over 26 protein bands were visualized upon sodium dodecyl sulfate-polyacrylamide g ...200717337566
development of a simple latex agglutination assay for detection of shiga toxin-producing escherichia coli (stec) by using polyclonal antibody against stec.rabbit antiserum raised against the whole-cell antigen of shiga toxin-producing escherichia coli (stec) strain vt3 (stx1+ stx2+ eae+) was repeatedly adsorbed with heat-killed cells of different non-stec strains and other enteric bacteria. thus, the antiserum obtained was designated vt3 antiserum. vt3 antiserum reacted with intimin type gamma. we assessed the reactivity of vt3 antiserum to whole-cell lysates of 87 strains of e. coli and other enteric bacteria by immunoblotting. the antiserum reco ...200717344348
a comparison of single oxidants versus advanced oxidation processes as chlorine-alternatives for wild blueberry processing (vaccinium angustifolium).advanced oxidation processes and single chemical oxidants were evaluated for their antimicrobial efficacy against common spoilage bacteria isolated from lowbush blueberries. predominant bacterial flora were identified using biochemical testing with the assessment of relative abundance using non-selective and differential media. single chemical oxidants evaluated for postharvest processing of lowbush blueberries included 1% hydrogen peroxide, 100 ppm chlorine, and 1 ppm aqueous ozone while advanc ...200717350128
seizure associated with total parenteral nutrition-related hypermanganesemia.the trace element manganese is usually supplied when total parenteral nutrition is used. however, long-term parenteral administration of manganese, which bypasses the normal regulatory mechanism, may cause hypermanganesemia. manganese poisoning presents clinically with parkinsonian-like symptoms and psychological changes. seizures are a rare presentation of this disease. this report describes a 10-year-old female who had received total parenteral nutrition for 3 months because of short bowel syn ...200717352953
proteorhodopsin photosystem gene expression enables photophosphorylation in a heterologous host.proteorhodopsins (prs) are retinal-containing proteins that catalyze light-activated proton efflux across the cell membrane. these photoproteins are known to be globally distributed in the ocean's photic zone, and they are found in a diverse array of bacteria and archaea. recently, light-enhanced growth rates and yields have been reported in at least one pr-containing marine bacterium, but the physiological basis of light-activated growth stimulation has not yet been determined. to describe more ...200717372221
rice endophyte pantoea agglomerans ys19 forms multicellular symplasmata via cell aggregation.pantoea agglomerans is characterized by the formation of multicellular symplasmata. one unanswered question regarding this bacterium is how these structures are formed. in this study, the rice diazotrophic endophyte p. agglomerans ys19 was selected for exploration of this theme. ys19 was labeled with green fluorescent protein and the resulting recombinant ys19::gfp was observed to grow only slightly more slowly (a decrease of 5.5%) than the wild-type strain, and to show high gfp label stability ...200717391364
pantoea agglomerans liver abscess presenting with a painful thigh.this case highlights that an acute myositis leading to rhabdomyolysis may occur as a rare complication of hepatic abscess and shows the benefit of early recognition of this possible association. a 70-year-old man presented with fever and lower limb myalgia, with laboratory evidence of acute renal failure secondary to rhabdomyolysis. blood cultures revealed pantoea agglomerans, which led to identification of a hepatic abscess on computed tomography scan. supportive care together with antibiotics ...200717413296
evaluation of two surface sampling methods for detection of erwinia herbicola on a variety of materials by culture and quantitative pcr.this research was designed to evaluate surface sampling protocols for use with culture and quantitative pcr (qpcr) amplification assay for detection of the gram-negative bacterial biothreat simulant erwinia herbicola on a variety of surface materials. surfaces selected for evaluation were wood laminate, glass and computer monitor screens, metal file cabinets, plastic arena seats, nylon seat cushions, finished concrete flooring, and vinyl tile flooring. laboratory and test chamber studies were pe ...200717416685
[pantoea agglomerans--pathogen of elytrigia repens and arrhenatherum elatius diseases].strains of pantoea agglomerans were isolated from the samples with symptoms of bacterial damage of various organs of elytrigia repens and arrhenatherum elatius. the isolated bacteria are aggressive to different extent for a host-plant and a number of cultivated plants. some problems of evolution of this bacterial species parasitism are discussed.200717427410
pantoea agglomerans, a plant pathogen causing human disease.we present 53 pediatric cases of pantoea agglomerans infections cultured from normally sterile sites in patients seen at a children's hospital over 6 years. isolates included 23 from the bloodstream, 14 from abscesses, 10 from joints/bones, 4 from the urinary tract, and 1 each from the peritoneum and the thorax. p. agglomerans was most associated with penetrating trauma by vegetative material and catheter-related bacteremia.200717442803
structural heterogeneity and environmentally regulated remodeling of francisella tularensis subspecies novicida lipid a characterized by tandem mass spectrometry.the structural characterization of environmentally-regulated lipid a derived from francisella tularensis subspecies novicida (fn) u112 is described using negative electrospray ionization with a linear ion trap fourier transform ion cyclotron resonance (it-ft-icr) hybrid mass spectrometer. the results indicate that a unique profile of lipid a molecular structures are synthesized in response to fn growth at 25 degrees c versus 37 degrees c. molecular species were found to be tetra-acylated, sharin ...200717446084
periodontal disease as reservoir for multi-resistant and hydrolytic enterobacterial species.this investigation aimed to isolate enteric rods from subgingival sites of patients presenting chronic periodontitis lesions, and to assess antimicrobial resistance and expression of hydrolytic enzymes.200717451514
pantoea agglomerans septicemia in three newborn infants.pantoea infections are rare in humans, especially in neonates. infections are usually associated with plant thorn injury or outbreaks traced to contaminated parenteral nutrition, intravenous anesthetics or packed erythrocytes. between 1st of january 1994 and 1st of june 2005, 125 of 6383 patients (2%) in a 24-bed level iii nicu became colonized with pantoea agglomerans. three newborns exhibited late-onset pantoea agglomerans septicemia and died. sporadic cases of pantoea agglomerans septicemia h ...200717468662
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