Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| student satisfaction with curriculum modifications in a french dental school. | since 1994, important modifications have been implemented in the dental curriculum in france, and an additional year has been included in the dental curriculum. the 1st year has remained unchanged; it is common to both medical and dental schools and leads to a selection procedure of 1 in 10 dental students. in the new curriculum, the dental student is engaged in a 5-year programme in dental school (years 2 to 6), as opposed to 4 years in the former programme (years 2 to 5). basically, this new c ... | 2000 | 11168473 |
| job strain, type a behavior pattern, and the prevalence of coronary atherosclerosis in japanese working men. | to examine the relation of type a behavior pattern and job strain to angiographically documented coronary stenosis. | 2000 | 11053607 |
| [breeding of arachidonic acid producting strain by ion implantation]. | with ion implantation, a high-yield arachidonic acid producting strain(mortierella alpina) i49-n18 was selected, whose biomass was 30.80 g/l. the lipid component of biomass was 25.8%, in which aa content was 45.37%. the results showed that selected high-yield strain was steady on 250 l fermentor, whose aa yield was 4.0 g/l. | 2000 | 11051823 |
| recovery of pathogenic measles virus from cloned cdna. | reverse genetics technology so far established for measles virus (mev) is based on the edmonston strain, which was isolated several decades ago, has been passaged in nonlymphoid cell lines, and is no longer pathogenic in monkey models. on the other hand, mevs isolated and passaged in the epstein-barr virus-transformed marmoset b-lymphoblastoid cell line b95a would retain their original pathogenicity (f. kobune et al., j. virol. 64:700-705, 1990). here we have developed mev reverse genetics syste ... | 2000 | 10864679 |
| [lps mutants of sinorhizobium meliloti and their nodulation competitiveness]. | four tn5-transposon lps mutants of sinorhizobium meliloti (tb9, tb29, ts22 and ts32) have been studied. each of four mutants has been established to contain a single insertion of tn5-transposon in its genome. all mutations are located on a chromosome. nodulation competitiveness (nc) of mutants towards the parent strain of s. meliloti cxm1-188 was investigated by resistant method using coinoculation of mutant and parent strain in the ratio 1:1. it was shown that nc was only 19-31% and 8-10%, for ... | 2000 | 10872284 |
| enhanced alkaline protease production in addition to alpha-amylase via constructing a bacillus subtilis strain. | bacillus subtilis bios11 strain was previously isolated and identified. this strain naturally produces a high level of alpha-amylase. the multicopy (ps1) plasmid that carries the complete alkaline protease apra gene was introduced to this host strain by transformation. the newly constructed strain was found to express the apra gene and produces a high level of alkaline protease. the level of alpha-amylase production was not affected compared with the parent strain. the ps1 plasmid in the new hos ... | 2000 | 10849799 |
| the role of tandem is dimers in is911 transposition. | using a combined in vivo and in vitro approach, we demonstrated that the transposition products generated by is911 from a dimeric donor plasmid are different from those generated from a plasmid monomer. when carried by a monomeric plasmid donor, free is911 transposon circles are generated by intra-is recombination in which one is end undergoes attack by the other. these represent transposition intermediates that undergo integration using the abutted left (irl) and right (irr) ends of the element ... | 2000 | 10760133 |
| expansion of epitope cross-reactivity by anti-idiotype modulation of the primary humoral response. | the primary humoral response produces antigen-specific antibodies so to clear the initial infection, and generates a population of corresponding memory cells to prevent infection by future encounters with the same pathogen. the continuous genetic modification of a pathogen's exterior, however, is one mechanism used to evade the immune defenses of its host. here we describe a novel means, involving anti-idiotypic antibodies, by which the host can counteract such pathogen genetic alterations by mo ... | 2000 | 10781835 |
| characterization and analysis of a new gene involved in glucose starvation response in enterococcus faecalis. | the genome sequence of enterococcus faecalis, led us to discover that gls24, encoding a general stress protein, seems to be in the second last position of a putative six-gene operon structure. interestingly, another gene named orf4 located just upstream from gls24 shows strong identity (72%) with this last. to determine the role of the orf4 gene in e. faecalis, we have constructed a mutant strain by homologous recombination. phenotypic analysis of these cells, reveals that orf4 is probably invol ... | 2000 | 10791725 |
| construction of mini-tn10luxabcam/ptac-ats and its use for developing a bacteriophage that transduces bioluminescence to escherichia coli o157:h7. | mini-tn10luxabcam/ptac-ats was constructed in order to develop a luciferase-transducing bacteriophage for detecting escherichia coli o157:h7. the transposon was designed to deliver a 3.6-kb insertion that confers n-decanal-dependent bioluminescence and resistance to chloramphenicol and was constructed using mini-tn10cam/ptac-ats in the plasmid pnk2884 and luxab from vibrio harveyi. phiv10, a temperate bacteriophage infecting common phage types of escherichia coli o157:h7, was mutagenized as a pr ... | 2000 | 10620680 |
| the introduction of strain and its effects on the structure and stability of t4 lysozyme. | in order to try to better understand the role played by strain in the structure and stability of a protein a series of "small-to-large" mutations was made within the core of t4 lysozyme. three different alanine residues, one involved in backbone contacts, one in side-chain contacts, and the third adjacent to a small cavity, were each replaced with subsets of the larger residues, val, leu, ile, met, phe and trp. as expected, the protein is progressively destabilized as the size of the introduced ... | 2000 | 10623513 |
| biology of attenuated modified vaccinia virus ankara recombinant vector in mice: virus fate and activation of b- and t-cell immune responses in comparison with the western reserve strain and advantages as a vaccine. | the modified vaccinia virus ankara (mva) strain is a candidate vector for vaccination against pathogens and tumors, due to safety concerns and the proven ability of recombinants based on this vector to trigger protection against pathogens in animals. in this study we addressed the fate of the mva vector in balb/c mice after intraperitoneal inoculation in comparison with that of the replication-competent western reserve (wr) strain by measuring levels of expression of the reporter luciferase gene ... | 2000 | 10623755 |
| analysis of capsid formation of human polyomavirus jc (tokyo-1 strain) by a eukaryotic expression system: splicing of late rnas, translation and nuclear transport of major capsid protein vp1, and capsid assembly. | human polyomavirus jc (jcv) can encode the three capsid proteins vp1, vp2, and vp3, downstream of the agnoprotein in the late region. jcv virions are identified in the nucleus of infected cells. in this study, we have elucidated unique features of jcv capsid formation by using a eukaryotic expression system. structures of jcv polycistronic late rnas (m1 to m4 and possibly m5 and m6) generated by alternative splicing were determined. vp1 would be synthesized from m2 rna, and vp2 and vp3 would be ... | 2000 | 10644357 |
| correlates of immune protection induced by live, attenuated, cold-adapted, trivalent, intranasal influenza virus vaccine. | the authors conducted a 2-year, multicenter, double-blind, placebo-controlled efficacy field trial of live, attenuated, cold-adapted, trivalent influenza vaccine administered by nasal spray to children 15-71 months old. overall, vaccine was 92% efficacious at preventing culture-confirmed infection by influenza a/h3n2 and influenza b. because influenza a/h1n1 did not cause disease during the years in which this study was conducted, the authors sought to determine vaccine efficacy and correlates o ... | 2000 | 10720541 |
| variability in plant-microbe interaction between lupinus lines and bradyrhizobium strains. | even though lupin (lupinus albus l.) is known to potentially fix 150-200 kg/ha nitrogen for the use of a succeeding crop, precise information about lupinxbradyrhizobium strain interaction under the climatic conditions prevalent in the mid-atlantic region of the united states is unknown. we conducted two greenhouse experiments with the objective of characterizing this symbiotic relationship and to evaluate potential interaction between bradyrhizobium strains and lupin lines. in the first experime ... | 2000 | 11074278 |
| structural and topological differences between a glycopeptide-intermediate clinical strain and glycopeptide-susceptible strains of staphylococcus aureus revealed by atomic force microscopy. | novel cell surface topography was revealed on cocci from a glycopeptide-intermediate staphylococcus aureus (gisa) clinical strain by using atomic force microscopy. the gisa isolate and its revertant had two parallel circumferential surface rings. one equatorial surface ring was observed in control strains. in vancomycin-susceptible strains, additional rings were formed in the presence of vancomycin. ring depth measurements also revealed striking differences between the gisa strain and susceptibl ... | 2000 | 11083659 |
| staphylococcus aureus protein a recognizes platelet gc1qr/p33: a novel mechanism for staphylococcal interactions with platelets. | the adhesion of staphylococcus aureus to platelets is a major determinant of virulence in the pathogenesis of endocarditis. molecular mechanisms mediating s. aureus interactions with platelets, however, are incompletely understood. the present study describes the interaction between s. aureus protein a and gc1qr/p33, a multifunctional, ubiquitously distributed cellular protein, initially described as a binding site for the globular heads of c1q. suspensions of fixed s. aureus or purified protein ... | 2000 | 10722602 |
| effect of physiological mechanical perturbations on intact human myocardial repolarization. | the objective of this study was to investigate the relationship between acute decreases in right ventricular volume during valsalva strain (with resultant changes in autonomic neural tone) and measures of local endocardial repolarization time independent of heart rate and autonomic neural tone. | 2000 | 10728350 |
| actinomycin production persists in a strain of streptomyces antibioticus lacking phenoxazinone synthase. | truncated fragments of the phenoxazinone synthase gene, phsa, were prepared by the pcr. the resulting fragments were cloned into conjugative plasmid pkc1132 and transferred to streptomyces antibioticus by conjugation from escherichia coli. two of the resulting constructs were integrated into the s. antibioticus chromosome by homologous recombination, and each of the resulting strains, designated 3720/pjse173 and 3720/pjse174, contained a disrupted phsa gene. strain 3720/pjse173 grew poorly, and ... | 2000 | 10770769 |
| absence of shedding of two b. abortus strains in goats after vaccination with live vaccines. | the objective of this study was to determine if b. abortus rough mutant strains rb51 and rfbk are eliminated in goat milk. thirty milk goats were divided into two groups. group i was inoculated with 4x10(10) cfu/ml of b.abortus rb51 strain and group ii with 1x10(9) cfu/ml of b. abortus rfbk strain by subcutaneous route in the right axilary region. milk samples were taken aseptically on a daily basis for the first 30 days and weekly for the second and third months. the samples were inoculated on ... | 2000 | 10825605 |
| [inducible expression of msp1 gene of plasmodium falciparum by a tetracycline controlled promoter in salmonella typhi cvd908 strain]. | to investigate the inducible expression of msp1 gene of plasmodium falciparum in salmonella typhi cvd908 vaccine strain using a tetracycline-controlled plteto promoter. | 2000 | 11372377 |
| characterization of n2o-producing xanthomonas-like isolates from biofilters as stenotrophomonas nitritireducens sp. nov., luteimonas mephitis gen. nov., sp. nov. and pseudoxanthomonas broegbernensis gen. nov., sp. nov. | a group of yellow-pigmented isolates from ammonia-supplied biofilters showed an unusual denitrification reaction. all strains reduced nitrite but not nitrate without production of nitrogen (n2). the only product found was nitrous oxide (n2o). the strains were divided into two clusters and one separate strain by their fatty acid profiles, which were similar to the fatty acid profiles of the genera xanthomonas and stenotrophomonas. analyses of the 165 rdna sequences showed that these clusters and ... | 2000 | 10826814 |
| an improved integration replacement/disruption method for mutagenesis of yeast essential genes. | we improved the integration replacement/disruption method (shortle, d., novic, p., and botstein, d. proc. natl. acad. sci. usa 81: 4889-4893, 1984) for isolating mutants in any of essential genes of the yeast saccharomyces cerevisiae by integrating mutagenized dna into the wild type gene of interest. we adopted this method to isolate temperature-sensitive mutants of the mpc1 gene encoding the yll031c orf. to facilitate integration of the mutagenic plasmid at a site near the 5' end of the orf, a ... | 2000 | 10846619 |
| [selection of high avermectins producing strain and identification of avermectin b1]. | three types of colony, powdery gray, white and bald, were isolated from streptomyces avermitilis atcc31272. among them, only the powdery grey one produces avermectins. sa-76 strain was selected from the powdery grey strain by mutation with high energy electric flow, and its avermectins titer attainde 100 micrograms/ml in shaking flask. avermectin b1 was extracted and purified from the mycelia of sa-76, and identified by uv, ir, 1h-nmr, 13c-nmr and mass spectra. after sa-76 strain was treated twi ... | 2000 | 10883272 |
| genetic relationships of infectious bronchitis virus isolates from mississippi broilers. | a 582-base pair segment located in the nucleocapsid protein terminal part of the s1 gene of 26 arkansas (ark)-type infectious bronchitis virus (ibv) isolates from mississippi broilers was amplified and sequenced. reverse transcription-polymerase chain reaction and cycle sequencing techniques were used to elucidate the genetic and deduced amino acid relationships among the isolates. analysis suggested that the nucleotide and amino acid sequences of the isolates were highly conserved, with greater ... | 2000 | 10737646 |
| comparison of soccer shin guards in preventing tibia fracture. | the goal of this study was to evaluate the effectiveness of a number of shin guards in protecting against tibia fracture in soccer players. a secondary purpose was to determine the relationship between the material and structural differences in shin guard design and the protection provided. twenty-three commercially available shin guards were tested on a model leg containing a synthetic tibia that had been calibrated against human cadaver specimens. each guard was categorized into one of four ma ... | 2000 | 10751000 |
| aerobic degradation of tetrachloroethylene by toluene-o-xylene monooxygenase of pseudomonas stutzeri ox1. | tetrachloroethylene (pce) is thought to have no natural source, so it is one of the most difficult contaminants to degrade biologically. this common groundwater pollutant was thought completely nonbiodegradable in the presence of oxygen. here we report that the wastewater bacterium pseudomonas stutzeri ox1 degrades aerobically 0. 56 micromol of 2.0 micromol pce in 21 h (vmax approximately 2.5 nmol min(-1) mg(-1) protein and km approximately 34 microm). these results were corroborated by the gene ... | 2000 | 10888848 |
| the complexity of protective immunity against liver-stage malaria. | sterile protective immunity against challenge with plasmodium spp. sporozoites can be induced in multiple model systems and humans by immunization with radiation-attenuated plasmodium spp. sporozoites. the infected hepatocyte has been established as the primary target of this protection, but the underlying mechanisms have not been completely defined. abs, cd8+ t cells, cd4+ t cells, cytokines (including ifn-gamma and il-12), and no have all been implicated as critical effectors. here, we have in ... | 2000 | 10903750 |
| [expression of glycoprotein gene of the rabies virus 3ag strain by e-3 deleted adenovirus recombinant]. | to analyze chinese rabies virus vaccine strain 3ag glycoprotein (gp) gene and further produce gp by e3-deleted human adenovirus recombinant. | 2000 | 11498699 |
| regulation of expression of the yiaklmnopqrs operon for carbohydrate utilization in escherichia coli: involvement of the main transcriptional factors. | the yiaklmnopqrs (yiak-s) gene cluster of escherichia coli is believed to be involved in the utilization of a hitherto unknown carbohydrate which generates the intermediate l-xylulose. transcription of yiak-s as a single message from the unique promoter found upstream of yiak is proven in this study. the 5' end has been located at 60 bp upstream from the atg. expression of the yiak-s operon is controlled in the wild-type strain by a repressor encoded by yiaj. no inducer molecule of the yiak-s op ... | 2000 | 10913096 |
| quantitative determination of the biodegradable polymer poly(beta-hydroxybutyrate) in a recombinant escherichia coli strain by use of mid-infrared spectroscopy and multivariative statistics. | fourier transform infrared (ftir) spectroscopy in combination with the partial least squares (pls) multivariative statistical technique was used for quantitative analysis of the poly(beta-hydroxybutyrate) (phb) contents of bacterial cells. a total of 237 replicate spectra from 34 samples were obtained together with gas chromatography-determined reference phb contents. using the pls regression, we were able to relate the infrared spectra to the reference phb contents, and the correlation coeffici ... | 2000 | 10919800 |
| biochemical characterization of recombinant subunits of type 2a protein phosphatase overexpressed in pichia pastoris. | methylotrophic yeast pichia pastoris was used for a medium-scale expression of structural (pr65/a) and catalytic (pp2ac) subunits of human type 2a protein phosphatase (pp2a). constructs encoding these subunits, which were designed to introduce eight histidines at their n-termini, were introduced into the km71 pichia strain by homologous recombination. recombinant proteins overproduced after methanol induction were purified from cell-free extracts by anion-exchange chromatography on deae-sepharos ... | 2000 | 10931206 |
| variation in response among individuals to antigenic sites on the ha protein of human influenza virus may be responsible for the emergence of drift strains in the human population. | eight convalescent human sera obtained from patients aged 3 to 14 years old, who were infected with influenza a(h3n2) virus during the 1990/1991 influenza season, were characterized by a binding assay with chimeric hemagglutinin (ha) proteins between influenza virus a/aichi/2/68 and a/kamata/14/91(h3n2) strains. these sera did not recognize the ha protein of the a/aichi/2/68 strain but recognized that of the a/kamata/14/91 strain. the binding assay revealed that these sera recognized only the ha ... | 2000 | 10936103 |
| [the properties of the cellular surface of yersinia pestis strain ev and its achromogenic variants]. | the comparative study of the properties of the surface of vaccine strain y. pestis ev and its achromogenic variants (av) differing from the initial strain by decreased immunogenicity and by the morphology of colonies, has been made. the achromogenicity of y. pestis colonies has been shown to correlate with the loss of the outer membrane protein with a molecular weight 22 kd. the synthesis of this protein is determined by chromosomal genes. av have been found to have different sensitivity to bact ... | 2000 | 10925864 |
| dimethylsulfone as a growth substrate for novel methylotrophic species of hyphomicrobium and arthrobacter. | dimethylsulfone is a major product of the chemical oxidation in the atmosphere of the principal biogenic sulfur gas, dimethylsulfide, but no studies have been reported on the mechanisms for its microbiological degradation. three novel strains of bacteria have been isolated from enrichment cultures provided with dimethylsulfone as the only carbon and energy substrate. these are novel facultatively methylotrophic species of hyphonmicrobium and arthobacter, capable of growth on a range of one-carbo ... | 2000 | 10896224 |
| analysis of rdxa and involvement of additional genes encoding nad(p)h flavin oxidoreductase (frxa) and ferredoxin-like protein (fdxb) in metronidazole resistance of helicobacter pylori. | metronidazole (mtz) is a critical ingredient of modern multidrug therapies for helicobacter pylori infection. mtz resistance reduces the effectiveness of these combinations. although null mutations in a rdxa gene that encodes oxygen-insensitive nad(p)h nitroreductase was reported in mtz-resistant h. pylori, an intact rdxa gene has also been reported in mtz-resistant h. pylori, suggesting that additional mtz resistance mechanisms exist in h. pylori. we explored the nature of mtz resistance among ... | 2000 | 10898687 |
| an outbreak of enterovirus 71 infection in taiwan, 1998. ii. laboratory diagnosis and genetic analysis. | an epidemic of enterovirus 71 (ev71) occurred in taiwan from april to december of 1998, with two peaks, one in june and the other in october. many enteroviruses were isolated in our laboratory from 258 cases during this outbreak. approximately half of the enteroviruses isolated were ev71 and one fifth were coxsackievirus a16. | 2000 | 10942089 |
| comparative nucleotide sequence analyses of the entire genomes of b95a cell-isolated and vero cell-isolated measles viruses from the same patient. | experimental infection of monkeys with the ic-b strain of measles virus (mv), which was isolated in marmoset b lymphoblastoid b95a cells from an acute measles patient, caused clinical signs typical for measles, while infection by the ic-v strain isolated in african green monkey kidney vero cells from the same patient did not cause any clinical signs in infected monkeys. the ic-b strain replicated only in b95a cells, whereas the ic-v strain replicated in both b95a and vero cells (3,6). to clarify ... | 2000 | 10949953 |
| comparative analyses of secondary gene products of 3-deoxy-d-manno-oct-2-ulosonic acid transferases from chlamydiaceae in escherichia coli k-12. | the waaa gene encoding the essential, lipopolysaccharide (lps)-specific 3-deoxy-dmanno-oct-2-ulosonic acid (kdo) transferase was inactivated in the chromosome of a heptosyltransferase i and ii deficient escherichia coli k-12 strain by insertion of gene expression cassettes encoding the waaa genes of chlamydia trachomatis, chlamydophila pneumoniae or chlamydophila psittaci. the three chlamydial kdo transferases were able to complement the knockout mutation without changing the growth or multiplic ... | 2000 | 10951204 |
| decontamination of a mycoplasma-infected chlamydia pneumoniae strain by pulmonary passage in scid mice. | we describe a procedure to eliminate contaminating mycoplasma from chlamydia pneumoniae (c. pneumoniae) cultures by pulmonary passage in severe combined immunodeficiency mice (scid). four weeks after experimental infection only c. pneumoniae could be cultured from the lungs of the infected animals while mycoplasma could not be detected any longer, as shown by pcr, culture and transmission electron microscopy (tem). | 2000 | 10959732 |
| analysis of 16s rrna and 51-kilodalton antigen gene and transmission in mice of ehrlichia risticii in virgulate trematodes from elimia livescens snails in ohio. | operculate snails (the family pleuroceridae: elimia livescens) were collected between june and october 1998 from a river in central ohio where repeated cases of potomac horse fever (phf) have occurred. of collected snails, consistently 50 to 80% carried a combination of cercariae and sporocysts of digenetic virgulate trematodes. the trematodes obtained from each snail were pooled and tested for ehrlichia risticii, the agent of phf, by nested pcr using primers specific to the 16s rrna gene. out o ... | 2000 | 10970382 |
| myocardial strain by doppler echocardiography. validation of a new method to quantify regional myocardial function. | myocardial strain is a measure of regional deformation, and by definition, negative strain means shortening and positive strain, elongation. this study investigates whether myocardial strain can be measured by doppler echocardiography as the time integral of regional velocity gradients, using sonomicrometry as reference method. | 2000 | 10973846 |
| concerted evolution of duplicate fla genes in campylobacter. | campylobacters have two similar copies (flaa and flab) of their flagellin gene. it has been hypothesized that the two copies can serve for antigenic phase variation. analysis of polymorphisms within aligned multiple dna sequences of the campylobacter flagellin genes revealed high pairwise homoplasy indexes between flab/flab pairs that were not observed between any flaa/flaa pairings or flaa/flab pairings. thus it seems there are constraints on the sequence of flab that distinguish it from flaa. ... | 2000 | 10974116 |
| therapeutic trial in experimental tegumentary leishmaniasis caused by leishmania (leishmania) amazonensis. a comparative study between mefloquine and aminosidine. | one hundred and eighty-two male inbred c57/bl/6 mice were infected with 3 x 10(6) leishmania (leishmania) amazonensis promastigotes of the mhom/br/ph8 strain by means of a subcutaneous injection in the right ear. the animals were separated in three groups: 1) oral mefloquine hydrochloride treatment (16 mg/kg/day/10 days), 2) intramuscular aminosidine (paromomycin) treatment (20 mg/kg/20 days) and 3) control. twenty-six mice of each treated group were sacrificed, one at the end of treatment (nine ... | 2000 | 10936952 |
| [search for staphylococcus aureus heterogeneously resistant to vancomycin (hetero-vrsa) in mrsa strains isolated from clinical samples during 1990s]. | hetero-vrsa was studied in 978 mrsa strains isolated from clinical samples during 1991 to 1998. although no vrsa was detected, 23 strains (2.4%) were identified as hetero-vrsa by the vancomycin-resistance using mu3 agar plate. the frequency of hetero-vrsa was not increased in the course of time. mic of the hetero-vrsa to vancomycin and teicoplanin was 1-2 micrograms/ml and 0.5-12 micrograms/ml, respectively. all of the hetero-vrsa strains were confirmed as a heterogeneous strain by a population ... | 2000 | 11019513 |
| interaction of the rabies virus p protein with the lc8 dynein light chain. | the rabies virus p protein is involved in viral transcription and replication but its precise function is not clear. we investigated the role of p (cvs strain) by searching for cellular partners by using a two-hybrid screening of a pc12 cdna library. we isolated a cdna encoding a 10-kda dynein light chain (lc8). lc8 is a component of cytoplasmic dynein involved in the minus end-directed movement of organelles along microtubules. we confirmed that this molecule interacts with p by coimmunoprecipi ... | 2000 | 11024151 |
| yeast krr1p physically and functionally interacts with a novel essential kri1p, and both proteins are required for 40s ribosome biogenesis in the nucleolus. | using a two-hybrid screening with tom1, a putative ubiquitin-ligase gene of saccharomyces cerevisiae, we isolated krr1, a homologue of human hrb2 (for human immunodeficiency virus type 1 rev-binding protein 2). to characterize the gene function, we constructed temperature-sensitive krr1 mutants and isolated two multicopy suppressors. one suppressor is rps14a, encoding a 40s ribosomal protein. the c-terminal-truncated rps14p, which was reported to have diminished binding activity to 18s rrna, fai ... | 2000 | 11027267 |
| [lipopolysaccharides of shigella sonnei]. | immunobiological properties of native lipopolysaccharides (lps) from virulent and avirulent strains of shigella sonnei bacteria (lps-v and lps-a, respectively) were studied. in avirulent bacteria, lps-v induced immunosuppressive activity specific of the virulent strain. lps of the avirulent strain, whereas lps-a lacked this property. native lps-v with immunosuppressive activity were isolated from the virulent strain by and immune affinity method. treatment of lps-v with phenol or tca abolished i ... | 2000 | 11042887 |
| comparative sequence analysis identified mutations outside the nsp4 cytotoxic domain of tissue culture-adapted atcc-wa strain of human rotavirus and a novel inter-species variable domain in its c-terminus. | complete nucleotide sequence of the tissue culture-adapted atcc-wa strain of human rotavirus nsp4 was determined. sequence analysis detected two alternate forms of the gene with a nucleotide difference at position 331 (a or g) in the coding sequence (nsp4-a or nsp4-g) leading to a change from neutral glutamine97 in nsp4-a to a positively charged arginine97 in nsp4-g originating from the same atcc-wa preparation. in addition to this, both forms of atcc-wa nsp4 revealed three mutations at nucleoti ... | 2000 | 11043941 |
| intercellular communication in helicobacter pylori: luxs is essential for the production of an extracellular signaling molecule. | individual bacteria of numerous species can communicate and coordinate their actions via the production, release, and detection of extracellular signaling molecules. in this study, we used the vibrio harveyi luminescence bioassay to determine whether helicobacter pylori produces such a factor. cell-free conditioned media from h. pylori strains 60190 and 26695 each induced >100-fold-greater luminescence in v. harveyi than did sterile culture medium. the h. pylori signaling molecule had a molecula ... | 2000 | 10816463 |
| candidate osmosensors from candida utilis and kluyveromyces lactis: structural and functional homology to the sho1p putative osmosensor from saccharomyces cerevisiae. | in saccharomyces cerevisiae, increases in external osmolarity evoke osmostress-induced signalling via the hog map kinase pathway. one of the upstream components of this signal transduction route is the putative osmosensor, sho1p. with the aim to elucidate the molecular basis of osmosensing in budding yeast, we have cloned sho1 homologues from candida utilis and kluyveromyces lactis which allowed determination of conserved domains of sho1p. results obtained from sequence comparisons, confirmed th ... | 2000 | 11118628 |
| mycobacterial growth indicator tube versus the proportion method on löwenstein-jensen medium for antibiotic susceptibility testing of mycobacterium tuberculosis. | the mycobacterial growth indicator tube, a reliable system for detection of mycobacterial growth, was compared with the reference proportion method on löwenstein-jensen medium for antibiotic susceptibility testing of mycobacterium tuberculosis. a total of 62 clinical strains and four reference strains of mycobacterium tuberculosis were tested for susceptibility to streptomycin, isoniazid, rifampicin and ethambutol. of these, 36 were susceptible to all four antibiotics and 30 were resistant to at ... | 2000 | 11205631 |
| inactivation of six genes from chromosomes vii and xiv of saccharomyces cerevisiae and basic phenotypic analysis of the mutant strains. | within the frame of the eurofan project, aimed at the functional analysis of the novel orfs revealed by the systematic sequencing of the saccharomyces cerevisiae genome, we have inactivated six orfs encoding putative proteins with unknown function in the two s. cerevisiae strains fy1679 and w303-1b. five orfs are located on chromosome vii (ygr250c, ygr251w, ygr260w, ygr262c, ygr263c) and one on chromosome xiv (ynl234w). the genes have been inactivated in the fy1679 strain by a strategy that make ... | 2000 | 10649454 |
| v gamma 1+ t cells suppress and v gamma 4+ t cells promote susceptibility to coxsackievirus b3-induced myocarditis in mice. | coxsackievirus b3 infections of c57bl/6 mice, which express the mhc class ii ia but not ie ag, results in virus replication in the heart but minimal myocarditis. in contrast, bl.tg.ealpha mice, which are c57bl/6 mice transgenically induced to express ie ag, develop significant myocarditis upon coxsackievirus b3 infection. despite this difference in inflammatory damage, cardiac virus titers are similar between c57bl/6 and bl.tg.ealpha mice. removing gammadelta t cells from either strain by geneti ... | 2000 | 11035049 |
| strain distribution in plated and unplated sheep tibia an in vivo experiment. | after plate osteosynthesis changes in bone biology and bone mechanics are observed in the plated bone segment. compromise of the vascular supply in the plate bed leads to a remodelling process and to a temporary porosity in the bone cortex underneath the plate. in addition, the plate takes over some of the physiological loading of the bone, which in turn alters the normal strain distribution of the cortical bone tissue. the aim of the present study is to determine the tissue deformation of the s ... | 2000 | 11052379 |
| isolation of a new insertion element of yersinia intermedia closely related to remnants of mobile genetic elements present on yersinia plasmids harboring the yop virulon. | a new insertion element present in two alleles, designated is1635.1 and is1635.2, was identified on a plasmid of a yersinia intermedia strain by hybridization with the yersinia enterocolitica pyv virulence plasmid. is1635.1 and is1635.2 are 861 bp long, carry imperfect inverted terminal repeats and possess a single open reading frame encoding a putative transposase of the is6 family. a truncated is1635 element is present immediately downstream of element is1635.2. the capacity of the is1635 elem ... | 2000 | 11094276 |
| the isolation and characterization in yeast of a gene for arabidopsis s-adenosylmethionine:phospho-ethanolamine n-methyltransferase. | saccharomyces cerevisiae opi3 mutant strains do not have the phospholipid n-methyltransferase that catalyzes the two terminal methylations in the phosphatidylcholine (pc) biosynthetic pathway. this results in a build up of the intermediate phosphatidylmonomethylethanolamine, causing a temperature-sensitive growth phenotype. an arabidopsis cdna library was used to isolate three overlapping plasmids that complemented the temperature-sensitive phenotype. phospholipid analysis showed that the presen ... | 2000 | 11115895 |
| strengths and limitations of molecular subtyping in a community outbreak of legionnaires' disease. | an epidemiological and microbiological investigation of a cluster of eight cases of legionnaires' disease in los angeles county in november 1997 yielded conflicting results. the epidemiological part of the investigation implicated one of several mobile cooling towers used by a film studio in the centre of the outbreak area. however, water sampled from these cooling towers contained l. pneumophila serogroup 1 of another subtype than the strain that was recovered from case-patients in the outbreak ... | 2000 | 11218211 |
| the ortholog of human ataxin-2 is essential for early embryonic patterning in c. elegans. | ataxin-2, the gene product of the human spinocerebellar ataxia type 2 (sca2) gene, is a protein of unknown function. ataxin-2 interacts with ataxin-2-binding-protein 1 (a2bp1), a member of a novel family of putative rna-binding proteins. because the sequences of ataxin-2 and a2bp1 are evolutionarily conserved, we investigated functional aspects and expression pattern in the nematode caenorhabditis elegans. human ataxin-2 has 20.1% amino acid identity and 43.9% similarity to its c. elegans orthol ... | 2000 | 11303786 |
| development of hyperfimbriated strains of vibrio cholerae o1. | the vibrio cholerae o1 and o139 fimbrillin genes (fima or msha) were amplified by polymerase chain reaction and cloned into an escherichia coli pcr vector. these clones were sequenced. the fima sequences were found to be identical between v cholerae o1 and o139. one of the plasmids was digested with ecor i and inserted into the ecor i site of pgex-3x. the plasmid pvpp thus obtained was transferred into strains of wild-type v cholerae o1 bgd17 (classical in biotype) and its fimbriated strain by e ... | 2000 | 10941926 |
| use of genetically engineered salmonella typhimurium oy1002/1a2 strain coexpressing human cytochrome p450 1a2 and nadph-cytochrome p450 reductase and bacterial o-acetyltransferase in sos/umu assay. | the major pathway of bioactivation of procarcinogenic heterocyclic aromatic amines (hcas) is cytochrome p450 1a2 (cyp1a2)-catalyzed n-hydroxylation and subsequent esterification by o-acetyltransferase (o-at). we have previously reported that an umu tester strain, salmonella typhimurium oy1001/1a2, endogenously coexpressing human cyp1a2 and nadph-p450 reductase (reductase), is able to detect the genotoxicity of some aromatic amines [aryal et al., 1999, mutat res 442:113-120]. to further enhance t ... | 2000 | 11013410 |
| identification, cloning, and initial characterization of rot, a locus encoding a regulator of virulence factor expression in staphylococcus aureus. | a chromosomal insertion of transposon tn917 partially restores the expression of protease and alpha-toxin activities to pm466, a genetically defined agr-null derivative of the wild-type staphylococcus aureus strain rn6390. in co-transduction experiments, transposon-encoded erythromycin resistance and a protease- and alpha-toxin-positive phenotype are transferred at high frequency from mutant strains to agr-null strains of s. aureus. southern analysis of chromosomal dna and sequence analysis of d ... | 2000 | 10809700 |
| [expression of the proopiomelanocortin gene in the hypophysis of nicag-line hypertensive rats]. | expression of the proopiomelanocortin (pomc) gene in nisag rats with inherited stress-induced arterial hypertension has been investigated by the reverse transcription-competitive pcr method. normotensive rats of the inbred wistar albino glaxo (wag) strain have been used as control. the effect of 3 h immobilization stress has been examined. the content of pomc mrna in pituitaries of stressed nisag rats exceeds that in non-stressed rats of the same strain by a factor of 2.4. rats of the wag strain ... | 2001 | 11436556 |
| chrysanthemyl diphosphate synthase: isolation of the gene and characterization of the recombinant non-head-to-tail monoterpene synthase from chrysanthemum cinerariaefolium. | chrysanthemyl diphosphate synthase (cppase) catalyzes the condensation of two molecules of dimethylallyl diphosphate to produce chrysanthemyl diphosphate (cpp), a monoterpene with a non-head-to-tail or irregular c1'-2-3 linkage between isoprenoid units. irregular monoterpenes are common in chrysanthemum cinerariaefolium and related members of the asteraceae family. in c. cinerariaefolium, cpp is an intermediate in the biosynthesis of the pyrethrin ester insecticides. cppase was purified from imm ... | 2001 | 11287653 |
| the implication of repeated versus continuous strain on nerve function in a rat forelimb model. | we studied the effect of repeated and continuous nerve strain using a rat forelimb model to investigate whether an innocuous level of strain applied continuously affects nerve function when applied repeatedly. we used the rat medial cord of the brachial plexus and assessed the effects of strain by studying nerve histology (blood-nerve barrier), function (grasping strength), and electrophysiology. continuous stretching was applied to the rat forelimb for 1 hour at 2 n. after this strain neither h ... | 2001 | 11466641 |
| recruitment of a foreign quinone into the a1 site of photosystem i. in vivo replacement of plastoquinone-9 by media-supplemented naphthoquinones in phylloquinone biosynthetic pathway mutants of synechocystis sp. pcc 6803. | interruption of the phylloquinone (phq) biosynthetic pathway by interposon mutagenesis of the mena and menb genes in synechocystis sp. pcc 6803 results in plastoquinone-9 (pq-9) occupying the a(1) site and functioning in electron transfer from a(0) to the fes clusters in photosystem (ps) i (johnson, t. w., shen, g., zybailov, b., kolling, d., reategui, r., beauparlant, s., vassiliev, i. r., bryant, d. a., jones, a. d., golbeck, j. h., and chitnis, p. r. (2000) j. biol. chem. 275, 8523-8530. we r ... | 2001 | 11470786 |
| analysis of the quality of protection induced by a porcine influenza a vaccine to challenge with an h3n2 virus. | antigenic drift of swine influenza a (h3n2) viruses away from the human a/port chalmers/1/73 (h3n2) strain, used in current commercial swine influenza vaccines, has been demonstrated in the netherlands and belgium. therefore, replacement of this human strain by a more recent swine h3n2 isolate has to be considered. in this study, the efficacy of a current commercial swine influenza vaccine to protect pigs against a recent dutch field strain (a/sw/oedenrode/96) was assessed. to evaluate the level ... | 2001 | 11557293 |
| gender specificity in the genetic determinants of peak bone mass. | peak bone mass is a major determinant of osteoporotic fracture risk. gender differences in peak bone mass acquisition are well recognized in humans and may account for a substantial share of the increased prevalence of fragility fractures in women compared with men. skeletal development is regulated by both heritable and environmental factors. experimental animal models provide a means to circumvent complicating environmental factors. in this study we examined the heritability of peak bone miner ... | 2001 | 11697792 |
| [vibrio cholerae in madagascar: study of a multiresistant strain]. | madagascar was cholera free until march 1999. the first case was reported in mahajanga, a north west coast harbor. ten months later and despite a massive use of tetracycline as prophylactic drug, cholera had reached every region of the island. all suspected cholera samples were analysed at the pasteur institute of madagascar where susceptibility to tetracycline was systematically performed. on february 2000, a multidrug resistant strain of v. cholerae was isolated. we studied this strain by perf ... | 2001 | 12471739 |
| nucleus accumbens dopamine depletions make animals highly sensitive to high fixed ratio requirements but do not impair primary food reinforcement. | it has been suggested that dopamine in nucleus accumbens is involved in the process of enabling organisms to overcome work-related response costs. one way of controlling work costs with operant schedules is to use fixed ratio schedules with different ratio requirements. in the present study, the effects of nucleus accumbens dopamine depletions were investigated using six schedules: fixed ratio 5, 20, 50, 100, 200, and 300. in the first three schedules the food reinforcement consisted of one 45 m ... | 2001 | 11530224 |
| long-term effects of feeding flaxseed-based diets. 1. egg production parameters, components, and eggshell quality in two strains of laying hens. | we used a split-plot design of five diets: control (corn-soy) with 3.8% ca, 10% flaxseed with 3.8% ca, 10% flaxseed with 4.5% ca, 10% flaxseed with 3.8% ca and 22,000 iu vitamin d3/kg, and 10% flaxseed with 4.5% ca and 22,000 iu vitamin d3/kg, and two strains of birds, dekalb delta (dd) and hy-line w-36 (hl), to evaluate long-term effects of flaxseed supplementation on egg production parameters. each of the five treatments was randomly assigned and replicated six times with five hens per replica ... | 2001 | 11599708 |
| changes in hepatitis c virus population in serum and peripheral blood mononuclear cells in chronically infected patients receiving liver graft from infected donors. | we have previously studied hepatitis c (hcv)-infected recipients of livers from hcv-infected donors and found that either the donor's strain or the recipient's strain predominate in serum. the current study was undertaken to determine whether these changes are complete and whether they are reflected in the population of virus associated with peripheral blood mononuclear cells (pbmcs). | 2001 | 11571446 |
| construction of a francisella tularensis two-dimensional electrophoresis protein database. | we have started the construction of a two-dimensional database of the proteome of francisella tularensis, a bacterium that is responsible for the highly pathogenic disease tularemia. the genome of this intracellular pathogen is not completely sequenced yet and, currently, information about only 66 proteins is available from ncbi database. we have analyzed the f. tularensis live vaccine strain by two-dimensional gel electrophoresis with immobilized ph 3-10 gradient in the first dimension and 9-16 ... | 2001 | 11681204 |
| a novel plasmid-mediated beta-lactamase that hydrolyzes broad-spectrum cephalosporins in a clinical isolate of klebsiella pneumoniae. | a new extended-spectrum beta-lactamase with an isoelectric point (pi) of 6.2 was detected in klebsiella pneumoniae f161 that was isolated from a patient with infection. this strain was highly resistant to the third or fourth generation cephalosporins such as ceftazidime, ceftriaxone, cefoperazone, and cefpirome. analysis of this strain by the double disk diffusion test showed synergies between amoxicillin-clavulanate (amx-ca) and cefotaxime, and amx-ca and aztreonam, which suggested that this st ... | 2001 | 11794541 |
| bovine staphylococcus aureus mastitis: strain recognition and dynamics of infection. | restriction enzyme fragmentation pattern (refp) analysis was used to recognise staphylococcus aureus strain variation in naturally occurring bovine subclinical mastitis. multiple colony refp analysis identified eight distinct strains of s. aureus in addition to the original strains a and b that were infused via the intramammary route, indicating that individual quarters of the udder may be colonised simultaneously by more than one strain of s. aureus. examination of multiple colonies per milk sa ... | 2001 | 11694041 |
| cellular and lipopolysaccharide fatty acid composition of the type strains of klebsiella pneumoniae, klebsiella oxytoca, and klebsiella nonpathogenic species. | the cellular and lipopolysaccharide (lps) fatty acid compositions of the type strains of klebsiella pneumoniae, k. oxytoca, k. terrigena, k. planticola, and "k. trevisanii" were studied. the cellular fatty acids of klebsiellae were presented by straight-chain saturated and monounsaturated, cyclopropane, and hydroxy fatty acids. hexadecanoic, methylenehexadecanoic, octadecenoic and hexadecenoic acids prevailed. the k. pneumoniae strain mainly differed from the strains of other species by two and ... | 2001 | 11785258 |
| role of interleukin (il)-1 type 1 receptor in mycobacterial infection. | it is important to gain a better understanding of il-1-mediated signaling events in mycobacterial infection. in order to clarify the role of il-1 receptor type 1 (il-1 r1) in il-1 r1, knockout (ko) mice were infected with either mycobacterium tuberculosis h37rv or kurono strain by the respiratory route, and their ability to control mycobacterial growth, pulmonary granuloma formation, and cytokine mrna expression was investigated. il-1 r1 ko mice developed significantly larger (p< 0.01) granuloma ... | 2001 | 11791667 |
| the estrous cycle affects pseudorabies virus (prv) infection of the cns. | previous work had suggested that mucosal immunity may be affected by the stage of the estrous cycle. here, susceptibility to a neurotropic virus infection at different stages of the estrous cycle was assessed in a rodent model after direct injection of the virus into visceral organs. in the first two experiments, female sprague-dawley rats were infected with pseudorabies virus (prv, bartha's k-strain) by injection into either the cervix or the kidney after monitoring their estrous cycle. after e ... | 2001 | 11223009 |
| [construction of cryptococcus neoformans cap70 transformation system]. | to construct a transformation system of cryptococcus neoformans capsule-deficient strain cap70. | 2001 | 11798961 |
| prolonged herpes zoster in a patient infected with the human immunodeficiency virus. | in 1983, varicella zoster virus (vzv) disease was first recognized in the context of infection with the human immunodeficiency virus (hiv). since that time, there have been many reports discussing the occurrence and clinical manifestations of hepes zoster in hiv-infected patients. we describe the development of prolonged herpes zoster in a patient with acquired immunodeficiency syndrome (aids) over the course of 104 days. viral isolates at the three different clinical stages of the skin lesions ... | 2001 | 11804069 |
| [temperature sensitive mutant of herpes simplex virus type 1. i. pathogenicity and immunogenicity]. | the aim of the study was to characterize biological features of the sensitive mutant of hsv-1, derived from mcintyre strain by numerous virus passages at lowered replication temperature (28 degrees c). pathogenicity of obtained ts mutant for inbred mice lines, cfw/pzh and balb/cpzh, was determined. statistically significant decrease in virulence of the mutant for these mouse lines was demonstrated, as compared with the native virus strain, propagated at 37 degrees c. immunogenic activity of ts m ... | 2001 | 11757407 |
| [expression of the ge gene of pseudorabies virus in insect cells]. | in order to develop a simple and safe test for the detection of vaccinated as well as wild type pseudorabies virus (prv) infected pigs, the modified ge gene of prv ea strain, obtained by cutting the 5' utr using pcr and dna recombinant technique, was inserted into baculovirus expression vector pfastbac 1, resulting the trans-position plamid pfe1.75. after homologous recombination, recombinant baculovirus rvbace1.75 was gained and high level expression of glycoprotein e (ge) was observed after th ... | 2001 | 11702707 |
| resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in acinetobacter baumannii strain bm4454. | multidrug-resistant strain acinetobacter baumannii bm4454 was isolated from a patient with a urinary tract infection. the adeb gene, which encodes a resistance-nodulation-cell division (rnd) protein, was detected in this strain by pcr with two degenerate oligodeoxynucleotides. insertional inactivation of adeb in bm4454, which generated bm4454-1, showed that the corresponding protein was responsible for aminoglycoside resistance and was involved in the level of susceptibility to other drugs inclu ... | 2001 | 11709311 |
| sequences and antimycoplasmic properties of longibrachins lgb ii and lgb iii, two novel 20-residue peptaibols from trichoderma longibrachiatum. | longibrachins are members of the class of natural aib-containing peptides designated as peptaibols. six longibrachins, lga i-iv and lgb ii and iii, were purified from a trichoderma longibrachiatum strain by a procedure employing several chromatography steps including reversed-phase hplc. the amino acid sequence determination was based on a combination of liquid secondary ion mass spectrometry (lsims) and two-dimensional 1h and 13c nmr spectroscopy. longibrachins are 20-residue peptaibols with a ... | 2001 | 11429993 |
| radioreceptor assays for sensitive detection and quantitation of saxitoxin and its analogues from strains of the freshwater cyanobacterium, anabaena circinalis. | toxic freshwater cyanobacteria can contaminate water supplies and adversely effect humans, agricultural livestock, and wildlife. toxicity is strain-specific so morphological observations alone cannot predict the hazard level. two microtiter plate based bioassays have emerged for measuring saxitoxin (stx) and its derivatives, commonly found in the freshwater cyanobacteria anabaena and aphanizomenon. they use radioactively labeled stx binding by sodium channels, stx's pharmacological target, or an ... | 2001 | 11348083 |
| identification and characterization of the trna:psi 31-synthase (pus6p) of saccharomyces cerevisiae. | to characterize the substrate specificity of the putative rna:pseudouridine (psi)-synthase encoded by the saccharomyces cerevisiae open reading frame (orf) ygr169c, the corresponding gene was deleted in yeast, and the consequences of the deletion on trna and small nuclear rna modification were tested. the resulting deltaygr169c strain showed no detectable growth phenotype, and the only difference in psi formation in stable cellular rnas was the absence of psi at position 31 in cytoplasmic and mi ... | 2001 | 11406626 |
| electrophoretic karyotyping of candida albicans strains isolated from premature infants and hospital personnel in a neonatal intensive care unit. | electrophoretic karyotyping was used to compare dna probes of yeasts isolated from blood of preterm neonates (n = 66) in a neonatal intensive care unit (nicu) and from the hands of healthy hospital personnel (n = 10). the yeasts were identified as candida albicans using standard laboratory methods. dna was extracted from yeasts and isolation of identical dna strains from the pairs nurse-neonate suggested that one nurse transmitted one yeast strain by her hands to three neonates. four neonates ha ... | 2001 | 11899481 |
| autoantigen glycoprotein 70 expression is regulated by a single locus, which acts as a checkpoint for pathogenic anti-glycoprotein 70 autoantibody production and hence for the corresponding development of severe nephritis, in lupus-prone pxsb mice. | retroviral envelope glycoprotein gp70 is present in the sera of immunologically normal and autoimmune-prone strains of mice. however, only lupus-prone mice spontaneously develop gp70-anti-gp70 immune complexes (gp70ic), and these have been implicated in the development of nephritis. we investigated the genetic factors that affect the production of both free serum gp70 and gp70ic in the lupus-prone bxsb mouse strain by analyzing (bxsb x (c57bl/10 x bxsb)f(1))- and (c57bl/10 x (c57bl/10 x bxsb)f(1 ... | 2001 | 11466397 |
| [studies on in vitro expression for gi gene of marek's disease in e. coli by pgex vector]. | glycoprotein i gene(gi) was amplified from genomic dna of marek's disease virus (mdv) 648 strain by polymerase chain reaction(pcr). pcr product was cloned into pgex-6p-1 according to the right open reading frame(orf). the expression of gst-gi fusion protein was studied in detail on many factors including temperature, timing and iptg. the curve for od600 and the growing time of the recombinant bacteria is also established., which is helpful to find the optimal inducing time. gst-gi fusion protein ... | 2001 | 12552804 |
| [identification and cloning of a novel full orf gene of p. falciparum pdz domain containing protein]. | to identify and clone a novel full orf gene of p. falciparum pdz domain containing protein (pfpcp) and study its expression at the erythrocytic stage. | 2001 | 12905862 |
| characterization of a mycobacterium intracellulare variant strain by molecular techniques. | this paper describes a mycobacterium intracellulare variant strain causing an unusual infection. several isolates obtained from an immunocompromised patient were identified as members of the mycobacterium avium complex (mac) by the commercial accuprobe system and biochemical standard identification. further molecular approaches were undertaken for a more accurate characterization of the bacteria. up to seven different genomic sequences were analyzed, ranging from conserved mycobacterial genes su ... | 2001 | 11724827 |
| meaa, a putative coenzyme b12-dependent mutase, provides methylmalonyl coenzyme a for monensin biosynthesis in streptomyces cinnamonensis. | the ratio of the major monensin analogs produced by streptomyces cinnamonensis is dependent upon the relative levels of the biosynthetic precursors methylmalonyl-coenzyme a (coa) (monensin a and monensin b) and ethylmalonyl-coa (monensin a). the meaa gene of this organism was cloned and sequenced and was shown to encode a putative 74-kda protein with significant amino acid sequence identity to methylmalonyl-coa mutase (mcm) (40%) and isobutyryl-coa mutase (icm) large subunit (36%) and small subu ... | 2001 | 11222607 |
| trichoderma reesei rho3 a homologue of yeast rh03 suppresses the growth defect of yeast sec15-1 mutation. | the trichoderma reesei gene, rho3, encoding the functional homologue of the saccharomyces cerevisiae small gtp-binding protein rho3p was cloned as a suppressor of the secretion-deficient mutation sec15-1 in yeast. the encoded protein showed 61% amino acid identity to the rho3 protein. rescue of the growth defect of a rho3 disruption strain by an expression vector carrying rho3 cdna confirmed the functional homology with the s. cerevisiae rho3 gene. in addition, overproduction of t. reesei rhoiii ... | 2001 | 11680821 |
| flagellar phase variation of salmonella enterica serovar typhimurium contributes to virulence in the murine typhoid infection model but does not influence salmonella-induced enteropathogenesis. | although salmonella enterica serovar typhimurium can undergo phase variation to alternately express two different types of flagellin subunit proteins, fljb or flic, no biological function for this phenomenon has been described. in this investigation, we constructed phase-locked derivatives of s. enterica serovar typhimurium that expressed only fljb (termed locked-on) or flic (termed locked-off). the role of phase variation in models of enteric and systemic pathogenesis was then evaluated. there ... | 2001 | 11292720 |
| isolation and characterization of a novel leaf-inhabiting osmo-, salt-, and alkali-tolerant yarrowia lipolytica yeast strain. | salt-excreting leaves of atriplex halimus plants harvested in the central negev highlands of israel were screened for yeasts inhabiting their surfaces. several aerobic, moderately salt- and alkali-tolerant yeasts were isolated. one of the isolates (tentatively designated s-8) was identified as yarrowia lipolytica (wick.) van der walt and arx, on the basis of its morphological, biochemical/physiological characteristics, and of quantitative chemotaxonomic and molecular marker analyses. however, th ... | 2001 | 11688215 |
| identification of genes associated with natural competence in helicobacter pylori by transposon shuttle random mutagenesis. | to identify genes involved in dna transformation, we generated 1500 insertion mutants of a helicobacter pylori strain by transposon shuttle mutagenesis. all mutant strains were screened for their frequency of natural transformation. a total of 20 mutant strains were found to exhibit a significantly decreased transformation frequency. dna sequencing revealed seven genetic loci, including the reported comb locus, hp0017 (a putative virb4 homologue) and five loci without database match (hp0015, hp1 ... | 2001 | 11689003 |
| [characteristics of mutants of streptomyces peucetius subsp. caesius atcc 27952 resistant to anthracycline antibiotics]. | daunorubicine (dnr) and doxorubicine (dxr) resistance to anthracycline antibiotics and antibiotical activity of the strain streptomyces peucetius subsp. caesius atcc 27952-2 and its daunorubicine- and doxorubicine-resistant (dnrr and dxrr) mutants have been studied. it has been found that strain s. peucetius subsp. caesius atcc 27952-2 is much more resistant to dxr than to dnr. it has been shown that frequency of appearance of spontaneous mutants of the strain s. peucetius subsp. caesius atcc 27 ... | 2001 | 11692678 |
| a definite case of spondylodiscitis caused by streptococcus equisimilis. | to shed light on the role of streptococcus equisimilis (se) in the pathogenesis of intervertebral disc infection, we report here a case of lumbar spondylodiscitis in a 37-year-old male caused by se, with identification of this strain by cultures from l4-l5 lumbar disc biopsy. intravenous therapy with penicillin and gentamycin combined with immobilization resulted in a rapid and complete recovery. the patient did not have underlying disease and showed no obvious history of exposure to animals. we ... | 2001 | 11579722 |
| a novel c-terminal kinesin is essential for maintaining functional acidocalcisomes in trypanosoma brucei. | kinesins are cytoskeletal motor proteins that play roles in a variety of fundamental cellular processes including cell division and the anterograde transport of vesicles and organelles. we purified, cloned, and functionally characterized in trypanosoma brucei a new member of the c-terminal kinesin family, tbkifc1. kinetic constants of the recombinant motor domain of tbkifc1 were estimated at 0.56 microm for the microtubule dissociation constant (k(d)) with a k(cat) of 0.2 s(-1). immunolocalizati ... | 2001 | 11581257 |
| reca-mediated rescue of escherichia coli strains with replication forks arrested at the terminus. | the recombinational rescue of chromosome replication was investigated in escherichia coli strains with the unidirectional origin orir1, from the plasmid r1, integrated within oric in clockwise (intr1(cw)) or counterclockwise (intr1(cc)) orientations. only the intr1(cc) strain, with replication forks arrested at the terminus, required reca for survival. unlike the strains with reca-dependent replication known so far, the intr1(cc) strain did not require recbcd, recf, recg, recj, ruvab, or sos act ... | 2001 | 11567007 |