Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| characterization of coronavirus rna polymerase gene products. | 1996 | 9026661 | |
| fimbriae extracts from enterotoxigenic escherichia coli strains of bovine and porcine origin with k99 and/or f41 antigens. | fimbriae extracts obtained using the thermal shock method, from bovine and porcine enterotoxigenic escherichia coli strains with k99 and/ or f41 antigens, were analyzed by sds-page, immunoblotting and haemagglutinating activity. three major protein bands with molecular weights 17 kda, 29.3 kda and 30.9 kda were detected depending on the strain assayed. a 17 kda band was identified as the fimbrial subunit for k99 fimbriae and was detected in strains of bovine and porcine origin. the 30.9 kda band ... | 1996 | 9054120 |
| pyridoxal 5'-phosphate binding of a recombinant rat serine: pyruvate/alanine:glyoxylate aminotransferase. | serine: pyruvate/alanine: glyoxylate aminotransferase in the liver is a class iv amino-transferase. the present study was undertaken to characterize the pyridoxal 5'-phosphate (plp) binding to a recombinant rat serine: pyruvate/alanine: glyoxylate aminotransferase (spt10), which is a homodimer of 44.4 kda subunits. purified spt10 exhibited absorption maxima at approximately 330 nm in addition to a 278 nm protein peak and a approximately 420 nm peak of plp bound via schiff base, and contained 0.5 ... | 1996 | 8797099 |
| molecular epidemiology of escherichia coli o157:h7 by pulsed-field gel electrophoresis and comparison with that by bacteriophage typing. | one hundred twenty-four escherichia coli o157:h7 isolates were characterized by pulse-field gel electrophoresis, bacteriophage typing, and pcr of verotoxin genes. diversity indices obtained--0.786 for phage types and 0.987 for pulsed-field gel electrophoresis types--demonstrated that phage typing falls below the critical value (0.9) required for confident interpretation of results. | 1996 | 8815116 |
| effect of supplementary feeding during the sucking period on net absorption from the small intestine of weaned pigs. | an intestinal perfusion technique was used to measure the effects of supplementary feeding (experiment 1) and temporary weaning (experiment 2) during the sucking period on the net absorption of fluid, sodium, chloride and potassium from the small intestine of pigs after weaning. the technique was also applied to control pigs which did not receive supplementary feed and were not temporarily weaned during the sucking period. paired intestinal segments were prepared at five sites along the small in ... | 1996 | 8819198 |
| late cut silage suspected of causing milk taint. | 1996 | 8825324 | |
| evaluation of a pcr for detection of actinobacillus pleuropneumoniae in mixed bacterial cultures from tonsils. | a pcr for the detection of actinobacillus pleuropneumoniae was evaluated. all of 102 field, isolates of a, pleuropneumoniae reacted in the pcr by amplification of a 985 bp product. no pcr amplification product was observed when examining strains of a. ureae, a. capsulatus, a. hominis, a. equuli, a, rossii, a. suis, escherichia coli, bordetella bronchiseptica. streptococcus suis, pasteurella haemolytica, pasteurella multocida, haemophilus parasuis, haemophilus taxon minor group, haemophilus taxon ... | 1996 | 8828126 |
| mapping of epitopes on porcine zona pellucida-3 alpha by monoclonal antibodies inhibiting oocyte-sperm interaction. | zona pellucida glycoprotein 3 alpha (zp3 alpha) has been designated as the primary sperm receptor ligand in porcine gamete interaction. in this study, epitopes were mapped on porcine zp3 alpha (pzp3 alpha) by using monoclonal antibodies (mabs) possessing in vitro contraceptive efficacy. using western blots, we tested recombinant pzp3 alpha fragments expressed as fusion proteins in escherichia coli and corresponding to pzp3 alpha precursor protein amino acid residues 18-142 (f1), 140-243 (f2), 23 ... | 1996 | 8828847 |
| purification and characterization of a component produced by lactobacillus fermentum that inhibits the adhesion of k88 expressing escherichia coli to porcine ileal mucus. | it has previously been shown that lactobacillus fermentum strain 104r releases compounds into its culture fluid that inhibit the adhesion of enterotoxigenic escherichia coli k88. the aim of the present study was to purify and identify this compound. judged by gel filtration, the compound was found to be approximately 1700 kda. the amount of active compound increased upon prolonged incubation, while the number of viable cells reduced, suggesting that the activity was coming from dead cells. as th ... | 1996 | 8852678 |
| three-dimensional structure of porcine kidney d-amino acid oxidase at 3.0 a resolution. | the x-ray crystallographic structure of porcine kidney d-amino acid oxidase, which had been expressed in escherichia coli transformed with a vector containing dao cdna, was determined by the isomorphous replacement method for the complex form with benzoate. the known amino acid sequence, fad and benzoate were fitted to an electron density map of 3.0 a resolution with an r-factor of 21.0%. the overall dimeric structure exhibits an elongated ellipsoidal framework. the prosthetic group, fad, was fo ... | 1996 | 8864836 |
| isotype and antibody specificity of spontaneously formed immunoglobulins in pig fetuses and germ-free piglets: production by cd5- b cells. | pig fetuses, colostrum-deprived newborns and germ-free (gf) piglets, animals in which b-cell development is not influenced by maternal regulatory factors, were employed to study the occurrence and specificity of natural antibodies (nab). serum immunoglobulins of all isotypes were found in 44-day-old fetuses (the gestation period in pigs lasts 114 days) and their level, with predominating igm, was increased during fetal ontogeny. in sera of fetuses at the end of embryonic life as well as of newbo ... | 1996 | 8881765 |
| splanchnic homeostasis during endotoxin challenge in the pig as assessed by microdialysis and tonometry. | the splanchnic region is particularly susceptible to shock. the purpose of this study was to evaluate microdialysis of the liver and small intestine as a monitor of splanchnic metabolic deterioration (elevation of lactate and hypoxanthine) in porcine endotoxic shock. tonometry of the small intestine was used as a reference. microdialysis probes (liver, ileum, and artery), tonometer, and pulmonary artery catheter were inserted. eight animals were given escherichia coli lipopolysaccharide endotoxi ... | 1996 | 8885084 |
| synthesis, processing, and oligomerization of bovine herpesvirus 1 ge and gi membrane proteins. | this study reports the identification and initial characterization of the precursors, modified forms, and oligomers of bovine herpesvirus 1 (bhv-1) gi and ge proteins with polyvalent rabbit serum specific for gi or ge. our experiments used the colorado strain of bhv-1 and mutant viruses with insertions of the escherichia coli lacz gene into the predicted ge and gi reading frames. we also translated the ge and gi open reading frames in vitro and expressed them in uninfected cells using eukaryotic ... | 1996 | 8892910 |
| oedema disease caused by o-rough escherichia coli. | 1996 | 8903022 | |
| effects of immune challenge, dietary energy density, and source of energy on performance and immunity in weanling pigs. | the objective of this study was to investigate the effects of nutrient density and dietary energy source on performance and immune function of weanling pigs that were either challenged or not challenged with escherichia coli lipopolysaccharide (lps). a basal diet was formulated to contain 14 g cp/mj de and 7 g lysine/100 g cp. sulfur amino acids, threonine and tryptophan were kept constant relative to lysine. experimental diets were mixed using 70 parts basal diet and either 30 parts starch or a ... | 1996 | 8904712 |
| induction of interleukin (il)-1beta and il-8 mrna expression in porcine macrophages by lipopolysaccharide from serpulina hyodysenteriae. | lipopolysaccharide (lps) is a classic inducer of inflammatory cytokines and is a key virulence factor for most gram-negative pathogens. the effect of phenol-water (lps) and butanol-water (endotoxin) extracts from serpulina hyodysenteriae on inflammatory cytokine mrna expression from porcine alveolar macrophages was investigated. the lps and endotoxin extracts from s. hyodysenteriae induced a dose-dependent expression of interleukin 1beta (il-1beta) and il-8 which was weak compared with the respo ... | 1996 | 8926114 |
| identification of ligand recognition sites in heat-stable enterotoxin receptor, membrane-associated guanylyl cyclase c by site-directed mutational analysis. | guanylyl cyclase c (star), a receptor protein for heat-stable enterotoxin (sta) elaborated by escherichia coli, is associated with and spans the plasma membrane of mammalian intestinal cells. the extracellular domain functions in the binding of sta and the association of each domain to an oligomeric form. two amino acid residues, arg-136 and asp-347, were identified as the residues binding to sta in the extracellular domain of pig star by site-directed mutagenesis and analysis of expression on 2 ... | 1996 | 8945558 |
| molecular cloning of a cdna encoding diacylglycerol kinase (dgk) in arabidopsis thaliana. | diacylglycerol kinase (dgk) synthesizes phosphatidic acid from diacylglycerol, an activator of protein kinase c (pkc), to resynthesize phosphatidylinositols. the structure of dgk has not been characterized in plants. we report the cloning of a cdna, catdgk1, encoding dgk from arabidopsis thaliana. the catdgk1 cdna contains an open reading frame of 2184 bp, and encodes a putative protein of 728 amino acids with a predicted molecular mass of 79.4 kda. the deduced atdgk1 amino acid sequence exhibit ... | 1996 | 8605313 |
| interaction of verotoxin 2e with pig intestine. | in pigs with edema disease, verotoxin 2e (vt2e) is produced in the intestine and transported to tissues, but neither the mechanism by which toxin passes through the intestine nor its failure to induce an enterotoxic reaction is understood. binding of vt2e to pig intestine was examined by enzyme-linked immunosorbent assay involving microvillus membranes (mvm) and crude mucus; thin-layer chromatographic overlay immunoassay with total lipids extracted from mvm; and indirect immunofluorescence of to ... | 1996 | 8613382 |
| expression of soluble cloned porcine pepsinogen a in escherichia coli. | a system for the production of soluble porcine pepsinogen a (ec 3.4.23.1) was developed by fusing the pepsinogen and thioredoxin genes and then expressing the fused product (trx-pg) in escherichia coli. the expressed fusion protein was purified using a combination of ion-exchange and hydrophobic chromatography. trypsin digestion of the fusion protein yielded pepsinogen which was one residue longer than the intrinsic length. acidification of either the fusion protein or pepsinogen (tryptic digest ... | 1996 | 8615812 |
| nucleotide sequence and expression in e. coli of the complete p4 type vp4 from a g2 serotype human rotavirus. | the complete sequence of a p4 type vp4 gene from a g2 serotype human rotavirus, is2, isolated in india has been determined. although the is2 vp4 is highly homologous to the other p4 type alleles, it contained acidic amino acid substitutions at several positions that make it acidic among the p4 type alleles that are basic. moreover, comparative sequence analysis revealed unusual polymorphism in members of the p4 type at amino acid position 393 which is highly conserved in members of other vp4 typ ... | 1996 | 8634023 |
| removal of map4 from microtubules in vivo produces no observable phenotype at the cellular level. | microtubule-associated protein 4 (map4) promotes mt assembly in vitro and is localized along mts in vivo. these results and the fact that map4 is the major map in nonneuronal cells suggest that map4's normal functions may include the stabilization of mts in situ. to understand map4 function in vivo, we produced a blocking antibody (ab) to prevent map4 binding to mts. the cooh-terminal mt binding domain of map4 was expressed in escherichia coli as a glutathione transferase fusion protein and was ... | 1996 | 8636213 |
| development and evaluation of an elisa to detect escherichia coli k88 (f4) fimbrial antibody levels. | an enzyme-linked immunosorbent assay (elisa) to determine igg antibody levels against k88 (f4) fimbrial antigen from porcine enterotoxigenic escherichia coli (etec) has been developed. the elisa method was checked with serum samples obtained from rabbits and pigs, and the parameters affecting the method were also analysed. elisa plates were optimally coated with k88 antigen 0.5 microgram/ml for testing rabbit antiserum or with 1.25 microgram/ml for testing pig serum. optimal concentrations of h2 ... | 1996 | 8636963 |
| glycans of bovine lactoferrin function as receptors for the type 1 fimbrial lectin of escherichia coli. | bovine lactoferrin strongly inhibited the hemagglutination activity of type 1 fimbriated escherichia coli. in addition, it agglutinated these bacteria. the agglutination reaction was specifically inhibited by glycopeptides derived from bovine lactoferrin or alpha-methyl-d-mannoside. these observations indicate that the glycans of bovine lactoferrin can serve as receptors for type 1 fimbrial lectin. | 1996 | 8641764 |
| role of the ceramide-signaling pathway in cytokine responses to p-fimbriated escherichia coli. | escherichia coli express fimbriae-associated adhesins through which they attach to mucosal cells and activate a cytokine response. the receptors for e. coli p fimbriae are the globoseries of glycosphingolipids; gal alpha 1-->4gal beta-containing oligosaccharides bound to ceramide in the outer leaflet of the lipid bilayer. the receptors for type 1 fimbriae are mannosylated glycoproteins rather than glycolipids. this study tested the hypothesis that p-fimbriated e. coli elicit a cytokine response ... | 1996 | 8642245 |
| inhibition of interleukin-6 synthesis in an animal model of septic shock by anti-c5a monoclonal antibodies. | the complement activation fragment c5a was recently shown to induce interleukin (il)-6 synthesis by peripheral blood mononuclear cells. to understand better the role of c5a in cytokine regulation in vivo, we investigated the effects of complement depletion by cobra venom factor (cvf) or of anti-c5a monoclonal antibodies (mab) on il-6 generation in an animal model of septic shock. complement-depleted pigs which were subsequently challenged with escherichia coli generated significantly (p < 0.05) ... | 1996 | 8647174 |
| a new approach to the treatment of experimental septic shock. | previous work has shown that disseminated intravascular coagulation (dic) may produce multiple organ failure, including adult respiratory distress syndrome, by obstruction of visceral micro circulation by microclots dic can be produced by sepsis. this study tests the ability of a plasminogen activator to prevent death from an intravenous injection of killed escherichia coli by causing lysis of the microclots. subjects were two groups of 8 pigs each with body weight of 60-70 lbs. killed escherich ... | 1996 | 8656601 |
| cloning and expression of pig kidney dopa decarboxylase: comparison of the naturally occurring and recombinant enzymes. | l-aromatic amino acid decarboxylase (dopa decarboxylase; ddc) is a pyridoxal 5'-phosphate (plp)-dependent homodimeric enzyme that catalyses the decarboxylation of l-dopa and other l-aromatic amino acids. to advance structure-function studies with the enzyme, a cdna that codes for the protein from pig kidney has been cloned by joining a partial cdna obtained by library screening with a synthetic portion constructed by the annealing and extension of long oligonucleotides. the hybrid cdna was then ... | 1996 | 8670114 |
| production of a human epidermal growth factor fusion protein and its degradation in rat gastrointestinal flushings. | this study describes the biosynthesis of a human epidermal growth factor fusion protein, long egf, that has a 53 amino acid extension peptide derived from the 46 n-terminal amino acids of porcine gh. the approach allowed the production of long egf at high efficiency due to the expression of the fusion protein in high yield as inclusion bodies in escherichia coli. long egf had a slightly lower potency compared with native egf in a range of assays, including binding to anti-egf antibodies or the e ... | 1996 | 8672237 |
| truncated enterohemorrhagic escherichia coli (ehec) o157:h7 intimin (eaea) fusion proteins promote adherence of ehec strains to hep-2 cells. | intimin, the product of the eaea gene in enterohemorrhagic escherichia coli o157:h7 (ehec), is required for intimate adherence of these organisms to tissue culture cells and formation of the attaching and effacing lesion in the gnotobiotic pig. because of the importance of intimin in the pathogenesis of ehec o157:h7 infection in this animal model, we began a structure-function analysis of eaea. for this purpose, we constructed amino-terminal fusions of the intimin protein with six histidine resi ... | 1996 | 8675331 |
| acute ethanol intoxication and endotoxemia after trauma. | to determine actions of acute intoxication on pathophysiologic responses to trauma, anesthetized and ventilated mongrel pigs received a 20% solution of ethanol (etoh) by an intravenous (iv group; 2 g/kg, n = 8) or an oral (po group; 3 g/kg, n = 12 x 60 minutes) route of administration, or the lactated ringer's vehicle (lr group; n = 12). after 60 minutes, all were subjected to soft tissue injury and 30 to 35% hemorrhage, 60-minute shock, and then resuscitation, with shed blood plus supplemental ... | 1996 | 8676425 |
| transferrin associated with the porcine intestinal mucosa is a receptor specific for k88ab fimbriae of escherichia coli. | putative receptors of escherichia coli k88 fimbriae are either tightly membrane bound or an integral part of membranes. thus, proteins associated with piglet small intestinal mucosae were solubilized by a detergent (deoxycholate). a 74-kda glycoprotein (gp74) purified from enterocyte and brush border membrane preparations was specifically detected in vitro by k88ab fimbriae. gp74 was recognized only in the mucosae of phenotypically adhesive animals. metaperiodate treatment abolished the recognit ... | 1996 | 8550214 |
| cloning and characterization of the eae gene from a dog attaching and effacing escherichia coli strain 4221. | we have cloned and determined the nucleotide sequence of the eae gene from a dog attaching and effacing (a/e) escherichia coli (depec) strain 4221. when comparing the predicted amino acid sequence of the eaedepec to that of the eae proteins from enteropathogenic e. coli (epec), enterohaemorrhagic e. coli o157:h7 (ehec), citrobacter freundii biotype 4280, and a swine a/e e. coli strain o45 (pepec), the overall sequence identity was 84, 81, 83 and 83%, respectively, with the greatest divergence at ... | 1997 | 9084152 |
| cardiovascular responses to glibenclamide during endotoxaemia in the pig. | the effects of blockading the atp-sensitive potassium channel (k+atp channels) on endotoxin-induced vascular derangements was studied. escherichia coli endotoxin was infused (20 micrograms/kg per h) intravenously for 180 min into anaesthetized, mechanically ventilated, indomethacin-treated pigs. after 150 min of endotoxaemia, glibenclamide (a k+ atp channel blocker) was infused intravenously at 2 mg/kg per min for 5 min. the cardiovascular parameters were recorded before (control), every 30 min ... | 1997 | 9090046 |
| development of selected faecal microfloras and of phagocytic and killing capacity of neutrophils in young pigs. | nine healthy piglets, weaned at the age of 35 days and emanating from two litters, were studied from 7 to 63 days of age. the development of their faecal flora was analysed by bacteriological quantification of coliforms, e. coli, enterococci and clostridium perfringens. the numbers of coliforms, e. coli and enterococci decreased from about 10(8) cfu/g faeces on day 7 to about 10(5) at the end of the study. clostridium perfringens, with an initial value of 10(4) cfu/g faeces, was not found in any ... | 1997 | 9100329 |
| colonization antigens of enterotoxigenic escherichia coli strains isolated from piglets in spain. | eighty-eight enterotoxigenic e.coli strains isolated from 69 pigs with enteric infections (diarrhoea or oedema disease) were investigated for the presence of f4 (k88), f5 (k99), f6 (987p) and f41 colonization antigens. the commonest colonization antigen was f6 (987p), which was detected in etec strains from 31.9% pigs, followed by f5 (k99) 11.6%, f4 (k88) 10.1% and f41 8.7%. presence of f6 (987p) and f5 (k99) fimbriae was statistically associated (0.025 > p < 0.005) with diarrhoeic piglets young ... | 1997 | 9100332 |
| nonaldehyde sterilization of biologic tissue for use in implantable medical devices. | biologic tissue stabilized by dye-mediated photooxidation has found application in implantable devices. the desire to avoid aldehydes in the processing of photooxidized tissues led to the development of a nonaldehyde, iodine based sterilant. the interaction of tissue with iodine was indicated by a change in tissue shrinkage temperature, dependent upon solution and incubation parameters. the amino acid tyrosine also was altered, presumably because of aromatic ring iodination. transmission electro ... | 1997 | 9116349 |
| effects of n omega-nitro-l-arginine methyl ester on the endotoxin-induced disseminated intravascular coagulation in porcine septic shock. | nitric oxide is known to prevent platelet aggregation and clot formation. inhibitors of nitric oxide synthase might promote or enhance endotoxin disseminated intravascular coagulation. the present study was designed to evaluate the effects of the arginine analog, n omega-nitro-l-arginine methyl ester (l-name), on the endotoxin-induced disseminated intravascular coagulation in a porcine model of septic shock. | 1997 | 9118662 |
| synthesis and antibacterial action of cecropin and proline-arginine-rich peptides from pig intestine. | two antimicrobial peptides, cecropin p1 (cp1), with a c-terminal carboxyl group, and pr-39, with an amidated, c-terminus, are found in the small intestine of the pig. each is active against both gram-positive and gram-negative bacteria. we have synthesized these peptides and several analogs, including the d-enantiomers and the retro sequences, each with a free or acetylated amino terminus. the cp1 amide was also prepared. the retro cp1 peptides were much less active than the parent cp1 peptide, ... | 1997 | 9128101 |
| 5-lipoxygenase and endotoxin-induced microvascular albumin exchanges and leucocyte recruitment in guinea-pig lungs. | the interference of the 5-lipoxygenase inhibitor, bw b70c ((e)-n-(3-[3-(4-fluorophenoxy)phenyl]-1(r,s)-methyl prop-2-enyl)-n-hydroxyurea), with escherichia coli lipopolysaccharide (endotoxin)-induced lung leucocyte sequestration and microvascular albumin exchanges was evaluated in the anaesthetised guinea-pig using radioactive tracers, in parallel to the effects on cell counts in the broncho-alveolar lavage fluid, blood tumour necrosis factor (tnf-alpha) content, secretion of phospholipase a2 an ... | 1997 | 9137918 |
| verotoxin-producing escherichia coli from the faeces of sheep, calves and pigs. | 1997 | 9140657 | |
| identification of cramp, a cathelin-related antimicrobial peptide expressed in the embryonic and adult mouse. | cathelicidins are the precursors of potent antimicrobial peptides that have been identified in several mammalian species. prior work has suggested that members of this gene family can participate in host defense through their antimicrobial effects and activate mesenchymal cells during wound repair. to permit further study of these proteins a reverse transcriptase-polymerase chain reaction approach was used to identify potential mouse homologs. a full-length 562-base pair cdna clone was obtained ... | 1997 | 9148921 |
| characterization of a large transferrin-binding protein from actinobacillus pleuropneumoniae serotype 7. | the binding of transferrin at the surface of actinobacillus pleuropneumoniae (a. pp.) is mediated by two proteins of approximately 60 and 100 kda. the 60 kda protein has been shown to be highly divergent among different serotypes and to induce a serotype-specific protective immune response. in this study we have characterized the 100 kda transferrin-binding protein of a. pp. serotype 7 and designated it as tfbb. the tfbb gene was found to be located immediately downstream of the tfba gene. it wa ... | 1997 | 9151535 |
| two oligomeric forms of plasma ficolin have differential lectin activity. | ficolins are plasma proteins with binding activity for carbohydrates, elastin, and corticosteroids. the ficolin polypeptide has a collagen-like domain that presumably brings three subunits together in a triple helical rod, a c-terminal fibrinogen-like domain (fbg) similar to that of tenascin, which presumably has the binding activities, and a small n-terminal domain that we find to be the primary site for forming the ficolin oligomer. by sedimentation equilibrium we determined that the main plas ... | 1997 | 9162054 |
| use of thermostable and escherichia coli rnase h in rna mapping studies. | a recently introduced thermostable rnase h was tested to determine its effectiveness in rnase h mapping reactions. procedures are described which should have general use with both the thermostable and the escherichia coli rnase h enzymes. using the thermostable rnase h at higher temperatures extends the range of oligodeoxyribonucleotide/rna combinations that yield satisfactory results. northern blot analyses of total rna was used to demonstrate that native rnas can be analyzed by oligodeoxyribon ... | 1997 | 9177689 |
| characterization of the molecular-chaperone function of the heat-shock-cognate-70-interacting protein. | a histidine-tagged form of the recently discovered molecular chaperone, 70-kda heat-shock cognate (hsc70)-interacting protein (hip), has been expressed in escherichia coli and purified to near homogeneity. this protein remains soluble when expressed in e. coli. several important properties of this chaperone have been investigated. hplc size-exclusion chromatography indicates that the chaperone forms a tetramer similar to what has been reported for the native protein from rat liver cytosol. the r ... | 1997 | 9183013 |
| effect of growth hormone or chromium picolinate on swine metabolism and inflammatory cytokine production after endotoxin challenge exposure. | to determine whether recombinant porcine somatotropin (pst) or chromium picolinate (crp) affected cytokine production and metabolism in swine after endotoxin challenge exposure. | 1997 | 9185964 |
| the endothelin receptor antagonist, bosentan, in combination with the cyclooxygenase inhibitor, diclofenac, counteracts pulmonary hypertension in porcine endotoxin shock. | to prevent endotoxin-induced pulmonary hypertension in the pig with a combination of a nonpeptide mixed endothelin receptor antagonist, bosentan, and a cyclooxygenase inhibitor, diclofenac. | 1997 | 9187606 |
| variation in virulence in the gnotobiotic pig model of o157:h7 escherichia coli strains of bovine and human origin. | escherichia coli strains of serotype o157:h7 have been incriminated in outbreaks and sporadic cases of food-borne illness, including diarrhea, hemorrhagic colitis, hemolytic uremic syndrome and thrombotic thrombocytopenic purpura. food-producing animals, particularly cattle, are believed to be reservoirs of the organism. whether all strains of bovine origin pose human health risk is unknown and was the impetus for this investigation. we compared the virulence of ten slt-i, slt-ii, and eae dna pr ... | 1997 | 9191990 |
| fimbrial colonisation factors f18ab and f18ac of escherichia coli isolated from pigs with postweaning diarrhea and edema disease. | during the last 5 years at least four new types of colonisation factors have been described in association with porcine postweaning diarrhea and edema disease strains of e. coli. recently, evidence was presented that these fimbrial factors are closely related to each other, and therefore the common denomination f18 was proposed. until now, two variants f18ab and f18ac were identified that can be distinguished by serology. alternatively, to circumvent elaborate growth conditions for the optimal e ... | 1997 | 9192010 |
| a novel developmental process of intestinal epithelial lesions in a calf infected with attaching and effacing escherichia coli. | a comparative study on the adhesion of attaching and effacing escherichia coli (aeec) to the enterocytes between the colon of a calf and the jejunum of a piglet showed differences in the developmental process of attaching and effacing (ae) lesions. in the calf, pedestals consisted of fused microvilli, while in the piglet they developed from the apical epithelial cell membranes after effacing microvilli. microvilli adjacent to the aeec attachment site were atrophic in the calf, whereas they were ... | 1997 | 9192365 |
| attenuation of endothelium-dependent hyperpolarizing factor by bacterial lipopolysaccharides. | endothelium-dependent hyperpolarizing factor (edhf) is an important contributor to agonist-induced vascular dilation. recent studies suggest that bacterial lipopolysaccharides attenuate endothelium-dependent dilation. whether or not this effect is mediated through inhibition of edhf is not known. we studied the in vitro influence of escherichia coli lipopolysaccharides on endothelium-dependent smooth muscle dilation and hyperpolarization in porcine coronary arteries. endothelium-intact porcine c ... | 1997 | 9203571 |
| expression of ovine interleukin-2 cdna in escherichia coli. | the expression plasmids pgex-2t and pt7-7 were used to express ovine (ov) il-2 cdna in escherichia coli. the pgex-2t vector contained glutathione-s-transferase (gst) as the affinity handle and resulted in high level expression of the gst-il-2 fusion protein. however, only a small proportion of this fusion protein was present in the soluble fraction. the insoluble fraction was extracted with a detergent, sarkosyl, and even though a large amount of fusion product was obtained, it would not bind to ... | 1997 | 9220585 |
| expression and phylogenetic relationships of a novel lacz homologue from actinobacillus pleuropneumoniae. | to learn more about the genetics and physiology of the important swine pathogen, actinobacillus pleuropneumoniae, we cloned the lacz gene by complementation of an escherichia coli delta lac mutant. the a. pleuropneumoniae lacz gene has an open reading frame of 3015 bp which could encode a protein with a predicted molecular mass of 117022. the deduced protein shares 26.8-34.8% identity with beta-galactosidases from both gram-positive and gram-negative bacteria. sequences with homology to seven re ... | 1997 | 9228778 |
| expression of p, s, and f1c adhesins by cytotoxic necrotizing factor 1-producing escherichia coli from septicemic and diarrheic pigs. | nineteen papc-positive cytotoxic necrotizing factor 1 (cnf1)-producing escherichia coli isolates from pigs with septicemia or diarrhea were tested for the presence of pap-, sfa-, and afa-related sequences encoding p/prs, s/f1c, and dr/afa adhesins respectively. production of adhesins by isolates was tested by mannose-resistant hemagglutination (mrha), sialidase treatment of erythrocytes and particle agglutination tests. production of p, s, and f1c fimbriae by isolates was also examined by immuno ... | 1997 | 9231424 |
| inotropic treatment and intestinal mucosal tissue oxygenation in a model of porcine endotoxemia. | to evaluate the dose-related effects of dopamine, dopexamine, and dobutamine on intestinal mucosal tissue oxygenation following short-time infusion of escherichia coli lipopolysaccharide, which has previously been shown to decrease mucosal tissue oxygenation by 60% of control values. | 1997 | 9233747 |
| epitope conservation and immunohistochemical localization of the guanylin/stable toxin peptide receptor, guanylyl cyclase c. | the heat-stable enterotoxins (st) are a family of cysteine-rich low-molecular weight peptides produced by pathogenic bacteria, and are one of the major causes of watery diarrhea all over the world. these toxins mediate their action by binding to an intestinal cell surface receptor that is a membrane-associated guanylyl cyclase (gcc). this receptor also serves as the receptor for the recently characterised endogenous ligand, guanylin. we have expressed various domains of the receptor in escherich ... | 1997 | 9282328 |
| effects of acute endotoxemia on serum somatotropin and insulin-like growth factor i concentrations in prepubertal gilts. | to evaluate effects of endotoxemia on serum somatotropin (st) and insulin-like growth factor i (igf-i) concentrations in finishing pigs. | 1997 | 9285006 |
| effect of transfusion on physiologic changes after resuscitated trauma. | the purpose of this experimental study was to test whether transfusion potentiated physiologic changes associated with fluid resuscitated trauma in controlled conditions. | 1997 | 9308610 |
| nitric oxide sensitivity of the aconitases. | aconitases are important cellular targets of nitric oxide (no.) toxicity, and no.-derived species, rather than no. per se, have been proposed to mediate their inactivation. no.-mediated inactivation of the escherichia coli aconitase and the porcine mitochondrial aconitase was investigated. in e. coli, aconitase activity decreased by approximately 70% during a 2-h exposure to an atmosphere containing 120 ppm no. in n2. the no.-inactivated aconitase reactivated poorly in e. coli under anaerobic or ... | 1997 | 9312115 |
| midgut-specific immune molecules are produced by the blood-sucking insect stomoxys calcitrans. | we have cloned and sequenced two defensins, smd1 and smd2, from anterior midgut tissue of the blood-sucking fly stomoxys calcitrans. the dna and n-terminal protein sequences suggest both are produced as prepropeptides. smd1 differs from the classic defensin pattern in having an unusual six-amino acid-long n-terminal sequence. both smd1 and smd2 have lower pi points and charge than insect defensins derived from fat body/hemocytes. northern analysis shows both of these defensin molecules are tissu ... | 1997 | 9326639 |
| genes coding for enterotoxins and verotoxins in porcine escherichia coli strains belonging to different o:k:h serotypes: relationship with toxic phenotypes. | seventy-four e. coli strains isolated from piglets with diarrhea or edema disease in spain were serotyped and examined for production of heat-labile (lt) and heat-stable (st) enterotoxins (lt-i, lt-ii, stah, stap, and stb) and verotoxins (vt1, vt2, and vt2v = vte) by phenotypic (vero cell assay and infant mouse test) and genotypic (colony hybridization and pcr) methods. in general, an excellent correlation was found between the results obtained with a pcr approach and those determined with biolo ... | 1997 | 9350767 |
| the influence of growth hormone on tumour necrosis factor and neutrophil leukocyte function in sepsis. | the aim of this study was to assess the influence of growth hormone (gh) in sepsis on the immune system represented by the circulating tnf-levels and the neutrophil leukocytes phagocytic capacity and respiratory burst, 22 piglets were randomized to 3 groups; pretreatment with gh (16 i.u.) before sepsis (n = 8), non-treated septic controls (n = 8), and non-septic controls (n = 6). sepsis was induced by a standardized infusion of live e. coli. tnf was measured by a cytotoxic bioassay, while neutro ... | 1997 | 9360256 |
| a 1-year study of escherichia coli o157 in cattle, sheep, pigs and poultry. | samples of rectal faeces were collected immediately after slaughter from 400 cattle each month for a 1-year period and from 1000 each of sheep, pigs and poultry over the same period. samples were examined for escherichia coli o157 by enrichment culture in buffered peptone water with vancomycin, cefixime and cefsulodin followed by immunomagnetic separation and culture of magnetic particles onto cefixime tellurite sorbitol macconkey agar. e. coli o157 was isolated from 752 (15.7%) of 4800 cattle, ... | 1997 | 9363024 |
| the use of a wild pig x domestic pig intercross to map phenotypic trait loci. | intercrosses between divergent lines of domestic animals adapted to different environmental conditions and/or production systems provide unique opportunities for mapping phenotypic trait loci. the strategy resembles the use of interspecific crosses developed for general linkage mapping. we have made an intercross between the wild pig and domestic pigs of the large white breed. a comprehensive linkage map comprising more than 230 genetic markers has been established. the successful use of this in ... | 1997 | 9378913 |
| immunological characterisation of glycoprotein e of aujeszky's disease virus. | a panel of 14 monoclonal antibodies (mabs) against glycoprotein e (ge) of aujeszky's disease (pseudorabies) virus (adv), which constitutes a representative sample of naturally occurring ge-specific antibodies in sera from infected animals, was produced and characterised. eleven topologically distinct antigenic domains represented by one or more mabs were identified on ge by using these mabs and three additional ge-specific mabs. three of the mabs available recognised conformation-independent epi ... | 1997 | 9381796 |
| inhibition of biliary endoprostheses occlusion by ampicillin-sulbactam in an in vitro model. | an important first step in stent occlusion is the formation of a bacterial biofilm. this is followed by deposition of granules similar to that found in brown pigment stones. previous in vitro models for studying occlusion have used synthetic biles without bilirubin or pooled human bile, which is limited in supply. our aim was to develop a new in vitro model of stent occlusion with porcine gallbladder bile and then, with the model, assess whether ampicillin-sulbactam can prevent biofilm formation ... | 1997 | 9422338 |
| detection of vtec using specific dna probes and complex typing of escherichia coli o157. | the incidence of e. coli causing hemorrhagic colitis (hc) or non-bloody enteritis in hungary was studied using slt-i and slt-ii gene-probes as well as vero-cell toxicity and verotox-f tests. out of 41 e. coli o157 strains isolated in hungary between 1987 and 1996 15 strains (o157:hnm 4, o157:h77 8, o157:hnt 3) derived from hemorrhagic colitis (hc). hybridization was observed with slt-i and/or slt-ii in 19 strains. verocytotoxin production of e. coli of 23 other serotypes was proven by hybridizat ... | 1997 | 9468730 |
| [a molecular test for the detection of e. coli f18 receptors: a breakthrough in the struggle against edema disease and post-weaning diarrhea in swine]. | oedema disease and post-weaning diarrhoea in swine are associated with the colonization of the intestine with toxigenic escherichia (e.) coli bacteria of various serotypes. colonization depends on specific binding between adhesive fimbriae and receptors on the enterocytes. the demonstration of these receptors allows the identification of susceptible and resistant pigs. direct sequencing of the alpha (1,2) fucosyltransferase gene (fut1) in swine being either susceptible or resistant to adhesion b ... | 1997 | 9480539 |
| glucosylation of small gtp-binding rho proteins disrupts endothelial barrier function. | the endothelial cytoskeleton is important for the regulation of endothelial barrier function. small gtp-binding rho proteins play a central role in the organization of the microfilament system. clostridium difficile toxin b (tcdb) inactivates rho proteins by glucosylation at thr-37. we used tcdb as a probe to study the role of rho proteins in the regulation of endothelial barrier function. tcdb time (50-170 min) and dose (10-100 ng/ml) dependently increased the hydraulic conductivity of cultured ... | 1997 | 9038900 |
| studies on the development and use of a monoclonal sandwich elisa for the detection of verotoxic escherichia coli in animal faeces. | a sandwich elisa using monoclonal antibodies to escherichia coli verocytotoxins 1 and 2 for capture and detection was developed for detection of verocytotoxin-producing escherichia coli (vtec) in animal faeces. for optimal toxin detection, the faeces were cultured in macconkey broth before subculture on to trypticase soy agar containing mitomycin c. it was possible to detect between 10 and 10(3) vti-producing and 1 to 10 vt2-producing e coli/g faeces, and overall the sensitivity and specificity ... | 1997 | 9042694 |
| immune response and growth of stressed weanling pigs fed diets supplemented with organic or inorganic forms of chromium. | a 2 x 4 factorial arrangement of treatments was used in a randomized complete block designed study to determine the effects of chromium level and source on growth and immune response of stressed and non-stressed 3-wk-old crossbred weanling pigs (bw was 6.35 kg). factors included 1) immune stress or control and 2) no supplemental cr or .2 ppm of supplemental cr from either crcl3, cr-picolinate, or cr-nicotinic acid complex. the basal diet was a corn-soybean meal-whey diet containing 1.2% lysine. ... | 1997 | 9051463 |
| in vivo and in vitro phosphorylation of rotavirus nsp5 correlates with its localization in viroplasms. | nsp5 (ns26), the product of rotavirus gene 11, is a phosphoprotein whose role in the virus replication cycle is unknown. to gain further insight into its function, we obtained monoclonal antibodies against the baculovirus-expressed protein. by immunoprecipitation and immunoblotting experiments, we showed that (i) nsp5 appears in many different phosphorylated forms in rotavirus-infected cells; (ii) immunoprecipitated nsp5 from rotavirus-infected cells can be phosphorylated in vitro by incubation ... | 1997 | 8985320 |
| molecular characterization of escherichia coli strains isolated from pigs with edema disease. | the present study was conducted to investigate the epidemiological relationship of isolates of escherichia coli causing edema disease. classical edema disease has not previously been described in denmark, but between february 1994 and november 1995 cases appeared in 51 pig herds, among which direct or indirect trading contacts were confirmed for 36 of the herds. a total of 213 isolates from pigs with edema disease in denmark and other countries and 23 e. coli o139 isolates from pigs with diarrhe ... | 1997 | 8968875 |
| frequency of adhesive factors and enterotoxins in strains of escherichia coli isolated from piglets with diarrhea. | neonatal colibacillosis is one of the most prevalent illnesses in pig farms. in order to examine the frequency of adherence factors and the production of enterotoxins in strains of e. coli, we collected stool specimens from 500 piglets between 1 and 10 days of age with diarrhea, including piglets from several different farms on the periphery of mérida, yucatán. one thousand and eighty (1080) strains of e. coli were isolated, of which 127 (11.76%) produced sta, and 62 (5.74%) produced adherence f ... | 1997 | 10932724 |
| hemolysins and verotoxin (vt) in enteric escherichia coli isolated from pigs. | one hundred and three e. coli strains isolated from the intestinal contents of pigs were examined for hemolysis and verotoxin production on vero monolayer cells. hemolysins were produced by 18 (17.5%) strains;--hemolysin was produced by 6 strains of which 4 belonged to serotype o149:k91, k88ac;--hemolysin was produced by 8 strains which could no be serotyped; 1 strain produced-hemolysin and possessed fimbrial antigens k88; the remaining 3 strains were enterohemolytic and vt producers. among the ... | 1997 | 10932726 |
| manure enhances plasmid mobilization and survival of pseudomonas putida introduced into field soil. | we report a field study on plasmid mobilization in an agricultural soil. the influence of pig manure on the mobilization of the incq plasmid pie723 by indigenous plasmids or by the incp(alpha) plasmid pgp527 into the recipient pseudomonas putida uwc1 (rif(supr) nal(supr)) was studied in field soil. six plots were prepared in duplicate, three of which were treated with manure prior to inoculation of the donor and recipient strains. as a donor strain, either escherichia coli j53(pie723) or e. coli ... | 1997 | 16535608 |
| s19 ribosomal protein cross-linked dimer causes monocyte-predominant infiltration by means of molecular mimicry to complement c5a. | s19 ribosomal protein is a component of the protein-producing machinery, ribosome. when recombinant s19 proteins were cross-linked intermolecularly by plasma transglutaminase (coagulation factor xiiia), this homodimer newly exhibited monocyte chemotactic activity. this effect was specific to monocytes. the s19 protein homodimer shared the immunoreactivity with the complement-derived chemotactic factor, component 5a (c5a). monocytes pretreated with an anti-c5a receptor antibody or with a syntheti ... | 1998 | 9881961 |
| hydrophilic thr can replace the hydrophobic and absolutely conservative a3val in insulin. | a mutant insulin, [a3thr]human insulin, was obtained by means of site-directed mutagenesis. the [a3thr]human insulin retains 50% receptor-binding potency and nearly total in vivo biological activity compared with native insulin, and can be crystallized using the same condition of native insulin. the results demonstrate that the absolutely conservative and hydrophobic valine at a3 can be substituted by hydrophilic threonine. | 1998 | 9920385 |
| the haemophilus influenzae htra protein is a protective antigen. | the htra gene from two strains of nontypeable haemophilus influenzae has been cloned and sequenced, and the encoded approximately 46-kda htra proteins were found to be highly conserved. h. influenzae htra has approximately 55% identity with the escherichia coli and salmonella typhimurium htra stress response proteins, and expression of the h. influenzae htra gene was inducible by high temperature. recombinant htra (rhtra) was expressed from e. coli, and the purified protein was found to have ser ... | 1998 | 9488373 |
| human and porcine aminoacylase i overproduced in a baculovirus expression vector system: evidence for structural and functional identity with enzymes isolated from kidney. | aminoacylase i (ec 3.5.1.14) is one of the most abundant enzymes in the cortical region of mammalian kidney. both the porcine and the human enzyme were overexpressed using baculovirus expression vector systems and purified by hydrophobic interaction chromatography and anion-exchange chromatography. the resulting preparations were analyzed for structural and functional identity with the corresponding enzymes isolated from kidney. the dansyl method as well as mass spectroscopy confirmed n-terminal ... | 1998 | 9518469 |
| characterization of the carbohydrate moiety of intestinal mucin-type sialoglycoprotein receptors for the k88ac fimbrial adhesin of escherichia coli. | we have previously identified two mucin-type sialoglycoproteins from porcine intestinal epithelial cells with approximate molecular masses of 210 (intestinal mucin-type glycoprotein imtgp-1) and 240 kda (imtgp-2) as receptors for the k88ab and k88ac fimbrial adhesins of escherichia coli. these receptors are detected in intestinal brush border membrane preparations from pigs with adhesive phenotypes but not from pigs with nonadhesive phenotypes and are postulated to be important determinants of t ... | 1998 | 9529089 |
| alkyl-dihydroxyacetonephosphate synthase. fate in peroxisome biogenesis disorders and identification of the point mutation underlying a single enzyme deficiency. | peroxisomes play an indispensible role in ether lipid biosynthesis as evidenced by the deficiency of ether phospholipids in fibroblasts and tissues from patients suffering from a number of peroxisomal disorders. alkyl-dihydroxyacetonephosphate synthase, a peroxisomal enzyme playing a key role in the biosynthesis of ether phospholipids, contains the peroxisomal targeting signal type 2 in a n-terminal cleavable presequence. using a polyclonal antiserum raised against alkyl-dihydroxyacetonephosphat ... | 1998 | 9553082 |
| phenotypic expression of k88 adhesion alone or simultaneously with k99 and/or f41 adhesins in the bovine enterotoxigenic escherichia coli strain b41. | f41-positive and f41-negative derivatives of bovine enterotoxigenic escherichia coli strain b41 carrying k88 or k88 and k99 plasmids were investigated for stability and expression of genes for their fimbrial antigens. either k88 plasmid alone or both k88 and k99 plasmids could be maintained in these strains though stability could depend on culture medium. k99 antigen could be detected in each strain bearing k99 plasmid. clones that produced k88 antigen or clones that did not produce this antigen ... | 1998 | 9556859 |
| inhibition of type i and type ii phospholipase a2 by phosphatidyl-ethanolamine linked to polymeric carriers. | we have previously shown that cell surface proteoglycans protect the cell membrane from the action of extracellular phospholipase a2 (pla2) enzymes [dan, p., nitzan, d. w., dagan, a., ginsburg, i., and yedgar, s. (1996) febs lett. 383, 75-78]. cell-impermeable pla2 inhibitors (explis) were prepared by linking phosphatidylethanolamine (pe) to polymeric carriers, specifically, carboxymethylcellulose, heparin, or hyaluronic acid. the structure of these inhibitors enables the incorporation of their ... | 1998 | 9558359 |
| hemodynamic effects of the laparoscopic pneumoperitoneum during sepsis in a porcine endotoxic shock model. | the authors compared the hemodynamic effects of laparoscopic intervention with conventional laparotomy in an endotoxic shock model in the pig. | 1998 | 9563532 |
| low molecular weight heparin prevents the pulmonary hemodynamic and pathomorphologic effects of endotoxin in a porcine acute lung injury model. | tumor necrosis factor alpha (tnf-alpha) activity, platelet and neutrophil degranulation and margination, and increased vascular permeability are central to the pathophysiology of endotoxin-mediated acute lung injury. nonanticoagulant activities of low molecular weight heparin (lmwh) include solubilization of the tnf-alpha receptor protein, inhibition of neutrophil adhesion, and regulation of thromboxane b2 (txb2) biosynthesis. in this study, we evaluated the ability of lmwh to modulate tnf-alpha ... | 1998 | 9565256 |
| expression of the glycoprotein e2 of the classical swine fever virus in escherichia coli. | the glycoprotein e2 sequences of classical swine fever virus (strain p97) were cloned, sequenced and expressed in e. coli. result from sds-polyacrylamide gel electrophoresis analysis of expressed proteins revealed the presence of a prominently stained band corresponding to a molecular mass of 61 kda, which is in agreement with the predicted size from the dna sequence. the recombinant e2 protein contained an aminoterminal tag of six histidines that could be used for purification by the nickel che ... | 1998 | 9592734 |
| increased platelet microvesicle formation is associated with mortality in a porcine model of endotoxemia. | gram-negative sepsis in humans and endotoxemia in pigs induce the formation of platelet microvesicles. these microvesicles are active in homeostasis and may thus contribute to the outcome in patients with activated coagulation and fibrinolysis. we decided to prospectively evaluate the effects of endotoxemia on microvesicle formation and some common physiologic variables against survival in a porcine model. | 1998 | 9605371 |
| prevalences of resistance to seven antimicrobials among fecal escherichia coli of swine on thirty-four farrow-to-finish farms in ontario, canada. | fecal specimens were composited and a hydrophobic-grid membrane-filter method was used to measure antimicrobial resistance to ampicillin 16 micrograms/ml, carbadox 30 micrograms/ml, gentamicin 4 mu/ml, nitrofurantoin 32 micrograms/ml, spectinomycin 16 micrograms/ml, sulfisoxazole 32 micrograms/ml and tetracycline 8 micrograms/ml among 8119 escherichia coli isolates from 68 fecal samples collected on 34 farrow-to-finish swine farms marketing over 500 hogs/yr. the overall prevalences of resistance ... | 1998 | 9618741 |
| in vitro disulfide-coupled folding of guanylyl cyclase-activating peptide and its precursor protein. | guanylyl cyclase-activating peptide ii (gcap-ii), an endogenous ligand of particulate guanylyl cyclase c (gc-c), is processed from the precursor protein and circulates in human blood. gcap-ii consists of 24 amino acid residues and contains two disulfide bridges. the correct disulfide paring of gcap-ii is an absolute requirement for its biological activity. this study shows that the folding of the peptide from the reduced form yields a peptide with the native disulfide paring as a minor product a ... | 1998 | 9622502 |
| factors affecting the heat resistance of escherichia coli o157:h7. | escherichia coli o157:h7 has been reported as being not particularly heat resistant. however, several factors which might increase its heat resistance have been investigated in this study using five strains. increase in growth temperature to 40 degrees c, as found in the cow gut, heat-shock at sub-lethal temperatures of 42, 45, 48 and 50 degrees c, and variable heating rate (1 degree c min-1 to 23 degrees c min-1) had no dramatic effect on heat resistance. growth phase had a marked impact on hea ... | 1998 | 9633099 |
| potent enzyme inhibitors derived from dromedary heavy-chain antibodies. | evidence is provided that dromedary heavy-chain antibodies, in vivo-matured in the absence of light chains, are a unique source of inhibitory antibodies. after immunization of a dromedary with bovine erythrocyte carbonic anhydrase and porcine pancreatic alpha-amylase, it was demonstrated that a considerable amount of heavy-chain antibodies, acting as true competitive inhibitors, circulate in the bloodstream. in contrast, the conventional antibodies apparently do not interact with the enzyme's ac ... | 1998 | 9649422 |
| detection of lawsonia intracellularis, serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, salmonella enterica, and haemolytic escherichia coli from swine herds with and without diarrhoea among growing pigs. | a polymerase chain reaction (pcr) was optimized to detect lawsonia intracellularis in faeces from naturally infected pigs. by combining a boiling procedure to extract dna and a nested pcr procedure, a detection limit at 2 x 10(2) bacterial cells per gram of faeces was achieved. the optimized pcr was used together with conventional culture techniques to detect serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes (wbhis), salmonella enterica, and haemolytic escherichia coli, in ... | 1998 | 9659692 |
| effect of induced hyperglycemia on brain cell membrane function and energy metabolism during the early phase of experimental meningitis in newborn piglets. | this study was done to elucidate the mechanism of hypoglycorrhachia and elevated lactate concentrations leading to neuronal dysfunction in neonatal meningitis, and to determine the effects of induced hyperglycemia on these disturbances. thirty-eight newborn piglets were divided into three groups: 12 in the control group (cg), 12 in the normoglycemic meningitis group (ng), and 14 in the hyperglycemic meningitis group (hg). meningitis was induced by intracisternal injection of 108 cfu of escherich ... | 1998 | 9666126 |
| mapping quantitative trait loci for immune capacity in the pig. | immune capacity traits show considerable genetic variation in outbred populations. to identify quantitative trait loci (qtls) for immune capacity in the pig, various measures of immune function (total and differential leukocyte counts, neutrophil phagocytosis, mitogen-induced proliferation, il-2 production, and virus induced ifn-alpha production in whole blood cultures, and ab responses to two escherichia coli antigens) were determined in 200 f2 animals from a wild pig-swedish yorkshire intercro ... | 1998 | 9670960 |
| pathogenic adaptation of escherichia coli by natural variation of the fimh adhesin. | conventional wisdom regarding mechanisms of bacterial pathogenesis holds that pathogens arise by external acquisition of distinct virulence factors, whereas determinants shared by pathogens and commensals are considered to be functionally equivalent and have been ignored as genes that could become adapted specifically for virulence. it is shown here, however, that genetic variation in an originally commensal trait, the fimh lectin of type 1 fimbriae, can change the tropism of escherichia coli, s ... | 1998 | 9671780 |
| virulence determinants of escherichia coli isolated from milk of sows with coliform mastitis. | escherichia coli isolates recovered from the milk of sows with coliform mastitis were examined to determine their biochemical and serologic characteristics, serum resistance and ability to adhere to fibronectin and bovine fetal fibroblasts. no common biotype was identified, and a variety of serovars were detected. ninety-five per cent of the investigated strains were serum resistant in swine serum. binding to fibronectin was demonstrated in most of the strains. at a binding level above 13% of th ... | 1998 | 9673580 |
| small heat shock protein of methanococcus jannaschii, a hyperthermophile. | small heat shock proteins (shsps) belong to a family of 12- to 43-kda proteins that are ubiquitous and are conserved in amino acid sequence among all organisms. a shsp homologue of methanococcus jannaschii, a hyperthermophilic archaeon, forms a homogeneous multimer comprised of 24 monomers with a molecular mass of 400 kda in contrast to other shsps that show heterogeneous oligomeric complexes. electron microscopy analysis revealed a spherically shaped oligomeric structure approximately 15-20 nm ... | 1998 | 9689045 |
| dietary zinc oxide in weaned pigs--effects on performance, tissue concentrations, morphology, neutrophil functions and faecal microflora. | the uptake and distribution of zinc in tissues and the effects of 2500 ppm dietary zinc oxide on health, faecal microflora, and the functions of circulating neutrophils were evaluated in weaned pigs. one group was fed a zinc supplement diet and another group was used as a control. all pigs remained healthy throughout the study, but the supplemented animals showed better performance than the controls. the serum zinc values rose rapidly. at autopsy, carried out at the age of 63 days, the zinc conc ... | 1998 | 9690608 |