Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| the phylogenetically-related pattern recognition receptors efr and xa21 recruit similar immune signaling components in monocots and dicots. | during plant immunity, surface-localized pattern recognition receptors (prrs) recognize pathogen-associated molecular patterns (pamps). the transfer of prrs between plant species is a promising strategy for engineering broad-spectrum disease resistance. thus, there is a great interest in understanding the mechanisms of prr-mediated resistance across different plant species. two well-characterized plant prrs are the leucine-rich repeat receptor kinases (lrr-rks) efr and xa21 from arabidopsis thal ... | 2015 | 25607985 |
| characterization of uronate dehydrogenases catalysing the initial step in an oxidative pathway. | uronate dehydrogenases catalyse the oxidation of uronic acids to aldaric acids, which represent 'top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. the identification and annotation of three uronate dehydrogenases derived from fulvimarina pelagi htcc2506, streptomyces viridochromogenes dsm 40736 and oceanicola granulosus dsm 15982 via sequence analysis is described. characterization and comparison with two known uronate dehydrogenases in regard to subst ... | 2015 | 25884328 |
| microbial diversity and activity in the nematostella vectensis holobiont: insights from 16s rrna gene sequencing, isolate genomes, and a pilot-scale survey of gene expression. | we have characterized the molecular and genomic diversity of the microbiota of the starlet sea anemone nematostella vectensis, a cnidarian model for comparative developmental and functional biology and a year-round inhabitant of temperate salt marshes. molecular phylogenetic analysis of 16s rrna gene clone libraries revealed four ribotypes associated with n. vectensis at multiple locations and times. these associates include two novel ribotypes within the ε-proteobacterial order campylobacterale ... | 2015 | 26388838 |
| root-associated bacterial endophytes from ralstonia solanacearum resistant and susceptible tomato cultivars and their pathogen antagonistic effects. | this study was undertaken to assess if the root-associated native bacterial endophytes in tomato have any bearing in governing the host resistance to the wilt pathogen ralstonia solanacearum. internal colonization of roots by bacterial endophytes was confirmed through confocal imaging after syto-9 staining. endophytes were isolated from surface-sterilized roots of 4-weeks-old seedlings of known wilt resistant (r) tomato cultivar arka abha and susceptible (s) cv. arka vikas on nutrient agar after ... | 2015 | 25926818 |
| a pterin-dependent signaling pathway regulates a dual-function diguanylate cyclase-phosphodiesterase controlling surface attachment in agrobacterium tumefaciens. | the motile-to-sessile transition is an important lifestyle switch in diverse bacteria and is often regulated by the intracellular second messenger cyclic diguanylate monophosphate (c-di-gmp). in general, high c-di-gmp concentrations promote attachment to surfaces, whereas cells with low levels of signal remain motile. in the plant pathogen agrobacterium tumefaciens, c-di-gmp controls attachment and biofilm formation via regulation of a unipolar polysaccharide (upp) adhesin. the levels of c-di-gm ... | 2015 | 26126849 |
| characterization of two-step deglycosylation via oxidation by glycoside oxidoreductase and defining their subfamily. | herein, we report a two-step deglycosylation mediated by the oxidation of glycoside which is different from traditional glycoside hydrolase (gh) mechanism. previously, we reported a novel flavin adenine dinucleotide (fad)-dependent glycoside oxidoreductase (fad-go) having deglycosylation activity. various features of the reaction of fad-go such as including mechanism and catalytic residue and substrate specificity were studied. in addition, classification of novel fad-go subfamily was attempted. ... | 2015 | 26057169 |
| paracoccus denitrificans possesses two bior homologs having a role in regulation of biotin metabolism. | recently, we determined that bior, the gntr family of transcription factor, acts as a repressor for biotin metabolism exclusively distributed in certain species of α-proteobacteria, including the zoonotic agent brucella melitensis and the plant pathogen agrobacterium tumefaciens. however, the scenario is unusual in paracoccus denitrificans, another closely related member of the same phylum α-proteobacteria featuring with denitrification. not only does it encode two bior homologs pden_1431 and pd ... | 2015 | 26037461 |
| the class iii cyclobutane pyrimidine dimer photolyase structure reveals a new antenna chromophore binding site and alternative photoreduction pathways. | photolyases are proteins with an fad chromophore that repair uv-induced pyrimidine dimers on the dna in a light-dependent manner. the cyclobutane pyrimidine dimer class iii photolyases are structurally unknown but closely related to plant cryptochromes, which serve as blue-light photoreceptors. here we present the crystal structure of a class iii photolyase termed photolyase-related protein a (phra) of agrobacterium tumefaciens at 1.67-å resolution. phra contains 5,10-methenyltetrahydrofolate (m ... | 2015 | 25784552 |
| structural characterization of antibiotic self-immunity trna synthetase in plant tumour biocontrol agent. | antibiotic-producing microbes evolved self-resistance mechanisms to avoid suicide. the biocontrol agrobacterium radiobacter k84 secretes the trojan horse antibiotic agrocin 84 that is selectively transported into the plant pathogen a. tumefaciens and processed into the toxin tm84. we previously showed that tm84 employs a unique trna-dependent mechanism to inhibit leucyl-trna synthetase (leurs), while the tm84-producer prevents self-poisoning by expressing a resistant leurs agnb2. we now identify ... | 2016 | 27713402 |
| low agrobacterium tumefaciens inoculum levels and a long co-culture period lead to reduced plant defense responses and increase transgenic shoot production of sunflower (helianthus annuus l.). | agrobacterium-mediated plant transformation is typically conducted by inoculating plant tissues with an agrobacterium suspension containing approximately 10(8)-10(9) bacteria ml(-1), followed by a 2-3-d co-culture period. use of longer co-culture periods could potentially increase transformation efficiencies by allowing more time for agrobacterium to interact with plant cells, but bacterial overgrowth is likely to occur, leading to severe tissue browning and reduced transformation and regenerati ... | 2016 | 27746666 |
| coordinated regulation of species-specific hydroxycinnamic acid degradation and siderophore biosynthesis pathways in agrobacterium fabrum. | the rhizosphere-inhabiting species agrobacterium fabrum (genomospecies g8 of the agrobacterium tumefaciens species complex) is known to degrade hydroxycinnamic acids (hcas), especially ferulic acid and p-coumaric acid, via the novel a. fabrum hca degradation pathway. gene expression profiles of a. fabrum strain c58 were investigated in the presence of hcas, using a c58 whole-genome oligoarray. both ferulic acid and p-coumaric acid caused variations in the expression of more than 10% of the c58 g ... | 2016 | 27060117 |
| agrobacterium tumefaciens zur regulates the high-affinity zinc uptake system trocba and the putative metal chaperone ycic, along with zint and znuabc, for survival under zinc-limiting conditions. | agrobacterium tumefaciens has a cluster of genes (atu3178, atu3179, and atu3180) encoding an abc-type transporter, here named troa, trob, and troc, respectively, which is shown here to be a zinc-specific uptake system. reverse transcription (rt)-pcr analysis confirmed that troa, trob, and troc are cotranscribed, with troc as the first gene of the operon. the ycic (atu3181) gene is transcribed in the opposite orientation to that of the trocba operon and belongs to a metal-binding gtpase family. e ... | 2016 | 27060116 |
| nicotine dehydrogenase complexed with 6-hydroxypseudooxynicotine oxidase involved in the hybrid nicotine-degrading pathway in agrobacterium tumefaciens s33. | nicotine, a major toxic alkaloid in tobacco wastes, is degraded by bacteria, mainly via pyridine and pyrrolidine pathways. previously, we discovered a new hybrid of the pyridine and pyrrolidine pathways in agrobacterium tumefaciens s33 and characterized its key enzyme 6-hydroxy-3-succinoylpyridine (hsp) hydroxylase. here, we purified the nicotine dehydrogenase initializing the nicotine degradation from the strain and found that it forms a complex with a novel 6-hydroxypseudooxynicotine oxidase. ... | 2016 | 26729714 |
| cyclic di-gmp regulates multiple cellular functions in the symbiotic alphaproteobacterium sinorhizobium meliloti. | sinorhizobium meliloti undergoes major lifestyle changes between planktonic states, biofilm formation, and symbiosis with leguminous plant hosts. in many bacteria, the second messenger 3',5'-cyclic di-gmp (c-di-gmp, or cdg) promotes a sessile lifestyle by regulating a plethora of processes involved in biofilm formation, including motility and biosynthesis of exopolysaccharides (eps). here, we systematically investigated the role of cdg in s. meliloti rm2011 encoding 22 proteins putatively associ ... | 2016 | 26574513 |
| the mosaic type iv secretion systems. | escherichia coli and other gram-negative and -positive bacteria employ type iv secretion systems (t4sss) to translocate dna and protein substrates, generally by contact-dependent mechanisms, to other cells. the t4sss functionally encompass two major subfamilies, the conjugation systems and the effector translocators. the conjugation systems are responsible for interbacterial transfer of antibiotic resistance genes, virulence determinants, and genes encoding other traits of potential benefit to t ... | 2016 | 27735785 |
| development of a microemulsion formulation for antimicrobial seca inhibitors. | in our previous study, we have identified five antimicrobial small molecules via structure based design, which inhibit seca of candidatus liberibacter asiaticus (las). seca is a critical protein translocase atpase subunit and is involved in pre-protein translocation across and integration into the cellular membrane in bacteria. in this study, eleven compounds were identified using similarity search method based on the five lead seca inhibitors identified previously. the identified seca inhibitor ... | 2016 | 26963811 |
| two distinct cardiolipin synthases operate in agrobacterium tumefaciens. | cardiolipin (cl) is a universal component of energy generating membranes. in most bacteria, it is synthesized via the condensation of two molecules phosphatidylglycerol (pg) by phospholipase d-type cardiolipin synthases (pld-type cls). in the plant pathogen and natural genetic engineer agrobacterium tumefaciens cl comprises up to 15% of all phospholipids in late stationary growth phase. a. tumefaciens harbors two genes, atu1630 (cls1) and atu2486 (cls2), coding for pld-type cls. heterologous exp ... | 2016 | 27472399 |
| regulatory activities of four arsr proteins in agrobacterium tumefaciens 5a. | arsr is a well-studied transcriptional repressor that regulates microbe-arsenic interactions. most microorganisms have an arsr gene, but in cases where multiple copies exist, the respective roles or potential functional overlap have not been explored. we examined the repressors encoded by arsr1 and arsr2 (ars1 operon) and by arsr3 and arsr4 (ars2 operon) in agrobacterium tumefaciens 5a. arsr1 and arsr4 are very similar in their primary sequences and diverge phylogenetically from arsr2 and arsr3, ... | 2016 | 27037117 |
| the antibacterial activity of chitosan products blended with monoterpenes and their biofilms against plant pathogenic bacteria. | this study focuses on the biological activities of eleven chitosan products with a viscosity-average molecular weight ranging from 22 to 846 kda in combination with the most active monoterpenes (geraniol and thymol), out of 10 tested, against four plant pathogenic bacteria, agrobacterium tumefaciens, erwinia carotovora, corynebacterium fascians, and pseudomonas solanacearum. the antibacterial activity was evaluated in vitro by the agar dilution technique as a minimum inhibitory concentration (mi ... | 2016 | 27127676 |
| involvement of agrobacterium tumefaciens galacturonate tripartite atp-independent periplasmic (trap) transporter gaapqm in virulence gene expression. | monosaccharides capable of serving as nutrients for the soil bacterium agrobacterium tumefaciens are also inducers of the vir regulon present in the tumor-inducing (ti) plasmid of this plant pathogen. one such monosaccharide is galacturonate, the predominant monomer of pectin found in plant cell walls. this ligand is recognized by the periplasmic sugar binding protein chve, which interacts with the vira histidine kinase that controls vir gene expression. although chve is also a member of the chv ... | 2016 | 26637603 |
| plant microbe interactions in post genomic era: perspectives and applications. | deciphering plant-microbe interactions is a promising aspect to understand the benefits and the pathogenic effect of microbes and crop improvement. the advancement in sequencing technologies and various 'omics' tool has impressively accelerated the research in biological sciences in this area. the recent and ongoing developments provide a unique approach to describing these intricate interactions and test hypotheses. in the present review, we discuss the role of plant-pathogen interaction in cro ... | 2016 | 27725809 |
| "cre/loxp plus bac": a strategy for direct cloning of large dna fragment and its applications in photorhabdus luminescens and agrobacterium tumefaciens. | heterologous expression has been proven to be a valid strategy for elucidating the natural products produced by gene clusters uncovered by genome sequencing projects. efforts have been made to efficiently clone gene clusters directly from genomic dna and several approaches have been developed. here, we present an alternative strategy based on the site-specific recombinase system cre/loxp for direct cloning gene clusters. a type three secretion system (t3ss) gene cluster (~32 kb) from photorhabdu ... | 2016 | 27364376 |
| arm-in-arm response regulator dimers promote intermolecular signal transduction. | bacteriophytochrome photoreceptors (bphps) and their cognate response regulators make up two-component signal transduction systems which direct bacteria to mount phenotypic responses to changes in environmental light quality. most of these systems utilize single-domain response regulators to transduce signals through unknown pathways and mechanisms. here we describe the photocycle and autophosphorylation kinetics of rtbphp1, a red light-regulated histidine kinase from the desert bacterium ramlib ... | 2016 | 26833410 |
| regulation of the cobalt/nickel efflux operon dmerf in agrobacterium tumefaciens and a link between the iron-sensing regulator rira and cobalt/nickel resistance. | the agrobacterium tumefaciens c58 genome harbors an operon containing the dmer (atu0890) and dmef (atu0891) genes, which encode a transcriptional regulatory protein belonging to the rcnr/csor family and a metal efflux protein belonging to the cation diffusion facilitator (cdf) family, respectively. the dmerf operon is specifically induced by cobalt and nickel, with cobalt being the more potent inducer. promoter-lacz transcriptional fusion, an electrophoretic mobility shift assay, and dnase i foo ... | 2016 | 27235438 |
| discrete responses to limitation for iron and manganese in agrobacterium tumefaciens: influence on attachment and biofilm formation. | transition metals such as iron and manganese are crucial trace nutrients for the growth of most bacteria, functioning as catalytic cofactors for many essential enzymes. dedicated uptake and regulatory systems have evolved to ensure their acquisition for growth, while preventing toxicity. transcriptomic analysis of the iron- and manganese-responsive regulons of agrobacterium tumefaciens revealed that there are discrete regulatory networks that respond to changes in iron and manganese levels. comp ... | 2016 | 26712936 |
| peptidomimetic small molecules disrupt type iv secretion system activity in diverse bacterial pathogens. | bacteria utilize complex type iv secretion systems (t4sss) to translocate diverse effector proteins or dna into target cells. despite the importance of t4sss in bacterial pathogenesis, the mechanism by which these translocation machineries deliver cargo across the bacterial envelope remains poorly understood, and very few studies have investigated the use of synthetic molecules to disrupt t4ss-mediated transport. here, we describe two synthetic small molecules (c10 and ksk85) that disrupt t4ss-d ... | 2016 | 27118587 |
| gqqa, a novel protein in komagataeibacter europaeus involved in bacterial quorum quenching and cellulose formation. | we report on the functional screening and identification of an active quorum quenching (qq) gene in the komagataeibacter europaeus strain cect 8546, which is a member of the acetic acid bacteria (aab). | 2016 | 27221658 |
| agrobacterium tumefaciens estc, encoding an enzyme containing esterase activity, is regulated by estr, a regulator in the marr family. | analysis of the a. tumefaciens genome revealed estc, which encodes an esterase located next to its transcriptional regulator estr, a regulator of esterase in the marr family. inactivation of estc results in a small increase in the resistance to organic hydroperoxides, whereas a high level of expression of estc from an expression vector leads to a reduction in the resistance to organic hydroperoxides and menadione. the estc gene is transcribed divergently from its regulator, estr. expression anal ... | 2016 | 28036400 |
| transcriptome analysis revealed that a quorum sensing system regulates the transfer of the pat megaplasmid in agrobacterium tumefaciens. | agrobacterium tumefaciens strain p4 is atypical, as the strain is not pathogenic and produces a for this species unusual quorum sensing signal, identified as n-(3-hydroxy-octanoyl)-homoserine lactone (3oh,c8-hsl). | 2016 | 27543103 |
| loss of podj in agrobacterium tumefaciens leads to ectopic polar growth, branching, and reduced cell division. | agrobacterium tumefaciens is a rod-shaped gram-negative bacterium that elongates by unipolar addition of new cell envelope material. approaching cell division, the growth pole transitions to a nongrowing old pole, and the division site creates new growth poles in sibling cells. the a. tumefaciens homolog of the caulobacter crescentus polar organizing protein popz localizes specifically to growth poles. in contrast, the a. tumefaciens homolog of the c. crescentus polar organelle development prote ... | 2016 | 27137498 |
| succinic semialdehyde promotes prosurvival capability of agrobacterium tumefaciens. | succinic semialdehyde (ssa), an important metabolite of γ-aminobutyric acid (gaba), is a ligand of the repressor attj regulating the expression of the attj-attklm gene cluster in the plant pathogen agrobacterium tumefaciens. while the response of a. tumefaciens to gaba and the function of attklm have been extensively studied, genetic and physiological responses of a. tumefaciens to ssa remain unknown. in combination with microarray and genetic approaches, this study sets out to explore new roles ... | 2016 | 26755630 |
| advancing crop transformation in the era of genome editing. | plant transformation has enabled fundamental insights into plant biology and revolutionized commercial agriculture. unfortunately, for most crops, transformation and regeneration remain arduous even after more than 30 years of technological advances. genome editing provides novel opportunities to enhance crop productivity but relies on genetic transformation and plant regeneration, which are bottlenecks in the process. here, we review the state of plant transformation and point to innovations ne ... | 2016 | 27335450 |
| purification, crystallization and structural elucidation of d-galactaro-1,4-lactone cycloisomerase from agrobacterium tumefaciens involved in pectin degradation. | pectin is found in the cell wall of plants and is often discarded as waste. a number of research groups are interested in redirecting this biomass waste stream for the production of fuel and bulk chemicals. the primary monomeric subunit of this polysaccharide is d-galacturonate, a six-carbon acid sugar that is degraded in a five-step pathway to central metabolic intermediates by some bacteria, including agrobacterium tumefaciens. in the third step of the pathway, d-galactaro-1,4-lactone is conve ... | 2016 | 26750482 |
| bacillus sp. qsi-1 modulate quorum sensing signals reduce aeromonas hydrophila level and alter gut microbial community structure in fish. | quorum sensing (qs) is a cell density dependent process that enables bacteria to communicate with each other based on the production, secretion and sensing of the auto-inducer molecules and then subsequently regulate virulence associated gene expression. interrupting quorum sensing may represent a novel alternative approach to combat bacterial pathogen. several bacteria can produce quorum quenching (qq) enzymes. however, the role of qq bacteria in shaping the microbiota and the level of n-acyl-h ... | 2016 | 28018866 |
| genome sequencing-assisted identification and the first functional validation of n-acyl-homoserine-lactone synthases from the sphingomonadaceae family. | members of the genus novosphingobium have been isolated from a variety of environmental niches. although genomics analyses have suggested the presence of genes associated with quorum sensing signal production e.g., the n-acyl-homoserine lactone (ahl) synthase (luxi) homologs in various novosphingobium species, to date, no luxi homologs have been experimentally validated. | 2016 | 27635318 |
| vgrg c terminus confers the type vi effector transport specificity and is required for binding with paar and adaptor-effector complex. | type vi secretion system (t6ss) is a macromolecular machine used by many gram-negative bacteria to inject effectors/toxins into eukaryotic hosts or prokaryotic competitors for survival and fitness. to date, our knowledge of the molecular determinants and mechanisms underlying the transport of these effectors remains limited. here, we report that two t6ss encoded valine-glycine repeat protein g (vgrg) paralogs in agrobacterium tumefaciens c58 specifically control the secretion and interbacterial ... | 2016 | 27313214 |
| use of random t-dna mutagenesis in identification of gene uvpro1, a regulator of conidiation, stress response, and virulence in ustilaginoidea virens. | false smut of rice, caused by ustilaginoidea virens (cooke) takahashi (teleomorph: villosiclava virens), is one of the most important diseases affecting rice worldwide. agrobacterium tumefaciens-mediated transformation was used to identify functional genes in u. virens. in this study, we selected a single-copy insertion mutant t133 with deficiency in producing conidia by screening the t-dna insertion mutant library of u. virens. the uvpro1-deletion mutant was successfully obtained after cloning ... | 2016 | 28082958 |
| uptake of the fusarium effector avr2 by tomato is not a cell autonomous event. | pathogens secrete effector proteins to manipulate the host for their own proliferation. currently it is unclear whether the uptake of effector proteins from extracellular spaces is a host autonomous process. we study this process using the avr2 effector protein from fusarium oxysporum f. sp. lycopersici (fol). avr2 is an important virulence factor that is secreted into the xylem sap of tomato following infection. besides that, it is also an avirulence factor triggering immune responses in plants ... | 2016 | 28066471 |
| deletion of afpab1 causes increased sensitivity to oxidative stress and hypovirulence in aspergillus fumigatus. | aspergillus fumigatus afpab1 is the ortholog of the aspergillus oryzae cytoplasmic messenger ribonucleoprotein granules aopab1 that function to depress the initiation of translation during stress. a. fumigatus can regulate its cellular physiology in response to environmental stresses, but there has been no research on pab1 in a. fumigatus. the associated gene afpab1 was replaced with a hygromycin-selectable marker to generate the strain δafpab1. phenotypic analysis showed that the δafpab1 grew m ... | 2016 | 27801871 |
| screening auxin response, in vitro culture aptitude and susceptibility to agrobacterium-mediated transformation of italian commercial durum wheat varieties. | the development of a robust agrobacterium-mediated transformation protocol for a recalcitrant species like durum wheat requires the identification and optimization of factors affecting t-dna delivery and plant regeneration. the purpose of this research was to compare the behavior of diverse durum wheat genotypes during in vitro culture and agrobacterium tumefaciens-mediated transformation, using immature embryos as explants. apart from plant genotype, two of the main influencing factors for a su ... | 2016 | 27801844 |
| generation of β-glucuronidase reporter-tagged strain to monitor ustilaginoidea virens infection in rice. | an agrobacterium-mediated genetic transformation system for the rice false smut fungus ustilaginoidea virens was developed using conidia as recipients. a binary vector, pcambia1301-pgpda-gus-ttrpc, was constructed. the gpda promoter (pgpda) from aspergillus nidulans was used to drive the expression of the β-glucuronidase (gus) gene which enabled gus activity visualization. the conidia transformation efficiency reached approximately 110 to 250 transformants per 1×10(5) conidia. based on the analy ... | 2016 | 27789314 |
| isolation of conditional mutations in genes essential for viability of cryptococcus neoformans. | discovering the genes underlying fundamental processes that enable cells to live and reproduce is a technical challenge, because loss of gene function in mutants results in organisms that cannot survive. this study describes a forward genetics method to identify essential genes in fungi, based on the propensity for agrobacterium tumefaciens to insert t-dna molecules into the promoters or 5' untranslated regions of genes and by placing a conditional promoter within the t-dna. insertions of the pr ... | 2016 | 27783209 |
| infiltration-rnaseq: transcriptome profiling of agrobacterium-mediated infiltration of transcription factors to discover gene function and expression networks in plants. | transcription factors (tfs) coordinate precise gene expression patterns that give rise to distinct phenotypic outputs. the identification of genes and transcriptional networks regulated by a tf often requires stable transformation and expression changes in plant cells. however, the production of stable transformants can be slow and laborious with no guarantee of success. furthermore, transgenic plants overexpressing a tf of interest can present pleiotropic phenotypes and/or result in a high numb ... | 2016 | 27777610 |
| characterization of vvpal-like promoter from grapevine using transgenic tobacco plants. | a 2000-bp 5'-flanking region of vvpal-like was isolated from 'summer black' grapevine by pcr amplification, named pvvpal-like. to gain a better understanding of the expression and regulatory mechanism of vvpal-like, a chimeric expression unit consisting of the β-glucuronidase (gus) reporter gene under the control of a 2000-bp fragment of the vvpal-like promoter was transformed into tobacco via agrobacterium tumefaciens. histochemical staining showed that the full-length promoter directs efficien ... | 2016 | 27562678 |
| biocontrol activity and patulin-removal effects of bacillus subtilis, rhodobacter sphaeroides and agrobacterium tumefaciens against penicillium expansum. | this study was conducted to evaluate the biocontrol potential of bacillus subtilis cicc 10034, rhodobacter sphaeroides cgmcc 1.2182 and agrobacterium tumefaciens cgmcc 1.2554 against patulin (pat)-producer penicillium expansum and their ability to remove pat. | 2016 | 27328641 |
| improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (medicago sativa l.) somatic embryos using cuscuta campestris extract. | developmental deficiency of somatic embryos and regeneration to plantlets, especially in the case of transformation, are major problems of somatic embryo regeneration in alfalfa. one of the ways to overcome these problems is the use of natural plant regulators and nutrients in the culture medium of somatic embryos. for investigating the influence of cuscuta campestris extract on the efficiency of plant regeneration and transformation, chimeric tissue type plasminogen activator was transferred to ... | 2016 | 27729718 |
| overexpressing arabidopsis abf3 increases tolerance to multiple abiotic stresses and reduces leaf size in alfalfa. | arabidopsis abscisic acid-responsive element-binding factor 3 (abf3), a bzip transcription factor, plays an important role in regulating multiple stress responses in plants. overexpressing atabf3 increases tolerance to various stresses in several plant species. alfalfa (medicago sativa l.), one of the most important perennial forage crops worldwide, has high yields, high nutritional value, and good palatability and is widely distributed in irrigated and semi-arid regions throughout the world. ho ... | 2016 | 27721135 |
| diverse profiles of n-acyl-homoserine lactones in biofilm forming strains of cronobacter sakazakii. | the present study investigates the role of quorum sensing (qs) molecules expressed by c. sakazakii in biofilm formation and extracellular polysaccharide expression. the qs signaling was detected using chromobacterium violaceum 026 and agrobacterium tumefaciens ntl4(pzlr4) based bioassay. long chain n-acyl-homoserine lactones (ahls) with c6- c18 chain length were identified using high performance liquid chromatography and liquid chromatography-high resolution mass spectrometry. a higher specific ... | 2016 | 27715454 |
| the nonspecific lipid transfer protein atltpi-4 is involved in suberin formation of arabidopsis thaliana crown galls. | nonspecific lipid transfer proteins reversibly bind different types of lipid molecules in a hydrophobic cavity. they facilitate phospholipid transfer between membranes in vitro, play a role in cuticle and possibly in suberin formation, and might be involved in plant pathogen defense signaling. this study focuses on the role of the lipid transfer protein atltpi-4 in crown gall development. arabidopsis (arabidopsis thaliana) crown gall tumors, which develop upon infection with the virulent agrobac ... | 2016 | 27688623 |
| identification of genes associated with asexual reproduction in phyllosticta citricarpa mutants obtained through agrobacterium tumefaciens transformation. | phyllosticta citricarpa is the epidemiological agent of citrus black spot (cbs) disease, which is responsible for large economic losses worldwide. cbs is characterized by the presence of spores (pycnidiospores) in dark lesions of fruit, which are also responsible for short distance dispersal of the disease. the identification of genes involved in asexual reproduction of p. citricarpa can be an alternative for directional disease control. we analyzed a library of mutants obtained through agrobact ... | 2016 | 27664732 |
| recombination-based generation of the agroinfectious clones of peanut stunt virus. | full-length cdna clones of peanut stunt virus strain p (psv-p) were constructed and introduced into nicotiana benthamiana plants via agrobacterium tumefaciens. the cdna fragments corresponding to three psv genomic rnas and satellite rna were cloned into pgreen binary vector between cauliflower mosaic virus (camv) 35s promoter and nopaline synthase (nos) terminator employing seamless recombinational cloning system. the plasmids were delivered into a. tumefaciens, followed by infiltration of hosts ... | 2016 | 27659243 |
| structural analysis and inhibition of trae from the pkm101 type iv secretion system. | gram-negative bacteria use type iv secretion systems (t4sss) for a variety of macromolecular transport processes that include the exchange of genetic material. the pkm101 plasmid encodes a t4ss similar to the well-studied model systems from agrobacterium tumefaciens and brucella suis here, we studied the structure and function of trae, a homolog of virb8 that is an essential component of all t4sss. analysis by x-ray crystallography revealed a structure that is similar to other virb8 homologs but ... | 2016 | 27634044 |
| construction and quality analysis of transgenic rehmannia glutinosa containing tmv and cmv coat protein. | plant viruses, especially tobacco mosaic virus (tmv) and cucumber mosaic virus (cmv) are serious threats to rehmannia glutinosa which is a "top grade" herb in china. in the present study, tmv- and cmv-resistant rehmannia glutinosa libosch. plants were constructed by transforming the protein (cp) genes of tmv and cmv into rehmannia glutinosa via a modified procedure of agrobacterium tumefaciens-mediated transformation. integration and expression of tmv cp and cmv cp transgenes in 2 lines, lba-1 a ... | 2016 | 27618888 |
| antitumor characterization of various fractions of launaea procumbens. | the antitumor activity of various six fractions of launaea procumbens were studied using the potato disk bioassay technique. three concentrations (10, 100, and 1000 ppm) of all these six fractions were used for characterization of antitumor activity. among these fractions, methanolic fraction exhibited significant inhibition of crown gall tumors caused by agrobacterium tumefaciens followed by butanolic fraction. these results revealed that methanolic and butanolic fractions contain bioactive con ... | 2016 | 24097358 |
| assessment of the function of sub6 in the pathogenic dermatophyte trichophyton mentagrophytes. | trichophyton mentagrophytes is a keratinophilic pathogenic fungus that infects both humans and animals. subtilisins are important for t. mentagrophytes virulence, particularly when invading the epidermal barrier of the host. subtilisin gene sub6 belongs to a seven-member gene family (sub1-sub7) encoding the subtilisin serine proteases. additionally, the sub6 gene product sub6, which is thought to be the major allergen tri r2 in trichophyton rubrum, elicits both immediate- and delayed-type hypers ... | 2016 | 26333355 |
| modification of flower colour by suppressing β-ring carotene hydroxylase genes in oncidium. | oncidium 'gower ramsey' (onc. gr) is a popular cut flower, but its colour is limited to bright yellow. the β-ring carotene hydroxylase (bch2) gene is involved in carotenoid biogenesis for pigment formation. however, the role of bch2 in onc. gr is poorly understood. here, we investigated the functions of three bch2 genes, bch-a2, bch-b2 and bch-c2 isolated from onc. gr, to analyse their roles in flower colour. rt-pcr expression profiling suggested that bch2 was mainly expressed in flowers. the ex ... | 2016 | 26404515 |
| ectopic overexpression of wssgtl1, a sterol glucosyltransferase gene in withania somnifera, promotes growth, enhances glycowithanolide and provides tolerance to abiotic and biotic stresses. | overexpression of sterol glycosyltransferase (sgtl1) gene of withania somnifera showing its involvement in glycosylation of withanolide that leads to enhanced growth and tolerance to biotic and abiotic stresses. withania somnifera is widely used in ayurvedic medicines for over 3000 years due to its therapeutic properties. it contains a variety of glycosylated steroids called withanosides that possess neuroregenerative, adaptogenic, anticonvulsant, immunomodulatory and antioxidant activities. the ... | 2016 | 26518426 |
| non-pathogenic rhizobium radiobacter f4 deploys plant beneficial activity independent of its host piriformospora indica. | the alphaproteobacterium rhizobium radiobacter f4 (rrf4) was originally characterized as an endofungal bacterium in the beneficial endophytic sebacinalean fungus piriformospora indica. although attempts to cure p. indica from rrf4 repeatedly failed, the bacterium can easily be grown in pure culture. here, we report on rrf4's genome and the beneficial impact the free-living bacterium has on plants. in contrast to other endofungal bacteria, the genome size of rrf4 is not reduced. instead, it shows ... | 2016 | 26495996 |
| inoculation of new rhizobial isolates improve nutrient uptake and growth of bean (phaseolus vulgaris) and arugula (eruca sativa). | in the current agricultural model, the massive use of chemical fertilizer causes environmental and economic losses. inoculation of plant-growth-promoting (pgp) nitrogen-fixing bacteria is an alternative to the use of nitrogen, phosphorus and potassium fertilizers. in this study, rhizobia strains isolated from common bean (phaseolus vulgaris) root nodules were evaluated in an effort to identify an efficient nitrogen-fixing rhizobia strain able to improve bean germination and growth. | 2016 | 26564333 |
| virus-induced gene silencing as a tool to study tomato fruit biochemistry. | virus-induced gene silencing (vigs) is an excellent reverse genetic tool for the study of gene function in plants, based on virus infection. in this chapter, we describe a high-throughput approach based on vigs for the study of tomato fruit biochemistry. it comprises the selection of the sequence for silencing using bioinformatics tools, the cloning of the fragment in the tobacco rattle virus (trv), and the agroinfiltration of tomato fruits mediated by agrobacterium tumefaciens. | 2016 | 26577782 |
| decreased expression of 14-3-3 in paracoccidioides brasiliensis confirms its involvement in fungal pathogenesis. | the interaction between the fungal pathogen paracoccidioides brasiliensis and host cells is usually mediated by specific binding events between adhesins on the fungal surface and receptors on the host extracellular matrix or cell surface. one molecule implicated in the p. brasiliensis-host interaction is the 14-3-3 protein. the 14-3-3 protein belongs to a family of conserved regulatory molecules that are expressed in all eukaryotic cells and are involved in diverse cellular functions. here, we i ... | 2016 | 26646480 |
| adaptation of the agrobacterium tumefaciens virg response regulator to activate transcription in plants. | the agrobacterium tumefaciens virg response regulator of the vira/virg two-component system was adapted to function in tobacco protoplasts. the subcellular localization of virg and vira proteins transiently expressed in onion cells was determined using gfp fusions. preliminary studies using gal4dbd-vp16 fusions with virg and escherichia coli uhpa, and narl response regulators indicated compatibility of these bacterial proteins with the eukaryotic transcriptional apparatus. a strong transcription ... | 2016 | 26646288 |
| polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following rubisco depletion and the removal of endotoxins. | the production of biopharmaceutical proteins in plants requires efficient downstream processing steps that remove impurities such as host cell proteins (hcps) and adventitious endotoxins produced by bacteria during transient expression. we therefore strived to develop effective routines for endotoxin removal from plant extracts and the subsequent use of the extracts to generate antibodies detecting a broad set of hcps. at first, we depleted the superabundant protein ribulose-1,5-bisphosphate car ... | 2016 | 26632519 |
| detection of quorum sensing molecules and biofilm formation in ralstonia solanacearum. | many bacteria use small diffusible signaling molecules to communicate each other termed as quorum sensing (qs). most gram-negative bacteria use acyl homoserine lactone (ahl) as qs signal molecules. using these signaling molecules, bacteria are able to express specific genes in response to population density. this work aimed to detect the production of qs signal molecules and biofilm formation in ralstonia solanacearum isolated from various diseased tomato plants with symptoms of bacterial wilt. ... | 2016 | 26620535 |
| transient protein expression by agroinfiltration in lettuce. | current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. however, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. in recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. newly develop ... | 2016 | 26614281 |
| multigene engineering in rice using high-capacity agrobacterium tumefaciens bibac vectors. | the high-capacity binary bacterial artificial chromosome (bibac) vector system permits the insertion of large fragments of dna, up to 150 kb, into plants via agrobacterium-mediated transformation. here, we describe an optimized protocol for transformation of japonica rice (oryza sativa l.) using this system. calli derived from mature embryos are transformed using agrobacterium strain lba4404 that carries the bibac vector and the super-virulent helper plasmid pch32. transformed calli are then reg ... | 2016 | 26614279 |
| constitutive expression of the tzs gene from agrobacterium tumefaciens virg mutant strains is responsible for improved transgenic plant regeneration in cotton meristem transformation. | key message : virg mutant strains of a nopaline type of agrobacterium tumefaciens increase the transformation frequency in cotton meristem transformation. constitutive cytokinin expression from the tzs gene in the virg mutant strains is responsible for the improvement. strains of agrobacterium tumefaciens were tested for their ability to improve cotton meristem transformation frequency. two disarmed a. tumefaciens nopaline strains with either a virgn54d constitutively active mutation or virgi77v ... | 2016 | 26650837 |
| cloning and evaluation of different constitutive promoters in the oleaginous yeast rhodosporidium toruloides. | the oleaginous yeast rhodosporidium toruloides is an unconventional yeast species that can accumulate a high content of lipids. because it belongs to the basidiomycetous group of fungus, limited tools and functional elements are available for genetic engineering of r. toruloides and related red yeasts. here we report the functional evaluation of five constitutive promoters from this yeast. we assembled a reporter gene expression cassette, consisting of a promoter, the hygromycin gene (hyg) and t ... | 2016 | 26648086 |
| rol genes enhance the biosynthesis of antioxidants in artemisia carvifolia buch. | the secondary metabolites of the artemisia genus are well known for their important therapeutic properties. this genus is one of the valuable sources of flavonoids and other polyphenols, but due to the low contents of these important metabolites, there is a need to either enhance their concentration in the original plant or seek alternative sources for them. the aim of the current study was to detect and enhance the yield of antioxidant compounds of artemisia carvifolia buch. hplc analysis was p ... | 2016 | 27251864 |
| the novel gene vppr4-1 from vitis pseudoreticulata increases powdery mildew resistance in transgenic vitis vinifera l. | pathogenesis-related proteins (prs) can lead to increased resistance of the whole plant to pathogen attack. here, we isolate and characterize a pr-4 protein (vppr4-1) from a wild chinese grape vitis pseudoreticulata which shows greatly elevated transcription following powdery mildew infection. its expression profiles under a number of abiotic stresses were also investigated. powdery mildew, salicylic acid, and jasmonic acid methyl ester significantly increased the vppr4-1 induction while nacl an ... | 2016 | 27303413 |
| thermodynamically optimal whole-genome tiling microarray design and validation. | microarray is an efficient apparatus to interrogate the whole transcriptome of species. microarray can be designed according to annotated gene sets, but the resulted microarrays cannot be used to identify novel transcripts and this design method is not applicable to unannotated species. alternatively, a whole-genome tiling microarray can be designed using only genomic sequences without gene annotations, and it can be used to detect novel rna transcripts as well as known genes. the difficulty wit ... | 2016 | 27295952 |
| the pharmaceutics from the foreign empire: the molecular pharming of the prokaryotic staphylokinase in arabidopsis thaliana plants. | here, we present the application of microbiology and biotechnology for the production of recombinant pharmaceutical proteins in plant cells. to the best of our knowledge and belief it is one of few examples of the expression of the prokaryotic staphylokinase (sak) in the eukaryotic system. despite the tremendous progress made in the plant biotechnology, most of the heterologous proteins still accumulate to low concentrations in plant tissues. therefore, the composition of expression cassettes to ... | 2016 | 27263008 |
| agrobacterium tumefaciens-mediated transformation of botryosphaeria dothidea. | botryosphaeria dothidea is a severe causal agent of die-back and cankers of many woody plants and causes great losses in many regions. the pathogenic mechanism of this pathogen has not been well explored due to lack of mutants and genetic information. in this study, we developed an agrobacterium tumefaciens-mediated transformation (atmt) protocol for b. dothidea protoplasts using vector pbht2 containing the hph gene as a selection marker under the control of trp c promoter. using this protocol w ... | 2016 | 27263001 |
| transient expression of p-type atpases in tobacco epidermal cells. | transient expression in tobacco cells is a convenient method for several purposes such as analysis of protein-protein interactions and the subcellular localization of plant proteins. a suspension of agrobacterium tumefaciens cells carrying the plasmid of interest is injected into the intracellular space between leaf epidermal cells, which results in dna transfer from the bacteria to the plant and expression of the corresponding proteins. by injecting mixes of agrobacterium strains, this system o ... | 2016 | 26695049 |
| interaction of antifungal peptide bp15 with stemphylium vesicarium, the causal agent of brown spot of pear. | peptide bp15 has shown antifungal activity against several plant pathogenic fungi, including stemphylium vesicarium, the causal agent of brown spot of pear. bp15 inhibits the germination, growth and sporulation of s. vesicarium and displays post-infection activity by stopping fungal infection in pear leaves. in this work, live-cell imaging was undertaken to understand the antifungal mechanism of bp15. a double-staining method based on the combination of calcofluor white and sytox green coupled w ... | 2016 | 26693685 |
| agrobacterium tumefaciens-mediated transformation: an efficient tool for insertional mutagenesis and targeted gene disruption in harpophora oryzae. | the endophytic filamentous fungus harpophora oryzae is a beneficial endosymbiont isolated from the wild rice. h. oryzae could not only effectively improve growth rate and biomass yield of rice crops, but also induce systemic resistance against the rice blast fungus, magnaporthe oryzae. in this study, agrobacterium tumefaciens-mediated transformation (atmt) was employed and optimized to modify the h. oryzae genes by either random dna fragment integration or targeted gene replacement. our results ... | 2016 | 26686612 |
| the essential role of spermidine in growth of agrobacterium tumefaciens is determined by the 1,3-diaminopropane moiety. | the ubiquitous polyamine spermidine is indispensable for eukaryotic growth and cell proliferation. a conserved vital function of spermidine across eukaryotes is conferred by its aminobutyl group that is transferred to a single lysine in translation factor eif5a to form the essential hypusine post-translational modification required for cellular translation. in direct contrast, although spermidine is absolutely essential for growth of α-proteobacterial plant pathogen agrobacterium tumefaciens, we ... | 2016 | 26682642 |
| advances in agrobacterium tumefaciens-mediated genetic transformation of graminaceous crops. | steady increase in global population poses several challenges to plant science research, including demand for increased crop productivity, grain yield, nutritional quality and improved tolerance to different environmental factors. transgene-based approaches are promising to address these challenges by transferring potential candidate genes to host organisms through different strategies. agrobacterium-mediated gene transfer is one such strategy which is well known for enabling efficient gene tran ... | 2016 | 26660352 |
| development of marker-free transgenic lettuce resistant to mirafiori lettuce big-vein virus. | lettuce big-vein disease caused by mirafiori lettuce big-vein virus (mlbvv) is found in major lettuce production areas worldwide, but highly resistant cultivars have not yet been developed. to produce mlbvv-resistant marker-free transgenic lettuce that would have a transgene with a promoter and terminator of lettuce origin, we constructed a two t-dna binary vector, in which the first t-dna contained the selectable marker gene neomycin phosphotransferase ii, and the second t-dna contained the let ... | 2016 | 27055463 |
| production and characterization of recombinant human acid α-glucosidase in transgenic rice cell suspension culture. | pompe disease is a fatal genetic muscle disorder caused by a deficiency of acid α-glucosidase (gaa), a glycogen-degrading lysosomal enzyme. in this study, the human gaa cdna gene was synthesized from human placenta cells and cloned into a plant expression vector under the control of the rice α-amylase 3d (ramy3d) promoter. the plant expression vector was introduced into rice calli (oryza sativa l. cv. dongjin) mediated by agrobacterium tumefaciens. genomic dna pcr and northern blot analysis were ... | 2016 | 27050503 |
| rnai-mediated resistance against cotton leaf curl disease in elite indian cotton (gossypium hirsutum) cultivar narasimha. | cotton leaf curl disease (clcud) is caused by several distinct begomovirus species in association with disease-specific betasatellite essential for induction of disease symptoms. clcud is a serious threat for the cultivation of cotton (gossypium sp.) and several species in the family malvaceae. in this study, rnai-based approach was applied to generate transgenic cotton (gossypium hirsutum) plants resistant to cotton leaf curl rajasthan virus (clcurv). an intron hairpin (ihp) rnai construct capa ... | 2016 | 27028623 |
| environment control to improve recombinant protein yields in plants based on agrobacterium-mediated transient gene expression. | agrobacterium-mediated transient expression systems enable plants to produce a wide range of recombinant proteins on a rapid timescale. to achieve economically feasible upstream production and downstream processing, two yield parameters should be considered: (1) recombinant protein content per unit biomass and (2) recombinant protein productivity per unit area-time at the end of the upstream production. because environmental factors in the upstream production have impacts on these parameters, en ... | 2016 | 27014686 |
| agrobacterium-mediated stable genetic transformation of populus angustifolia and populus balsamifera. | the present study demonstrates agrobacterium tumefaciens-mediated stable genetic transformation of two species of poplar - populus angustifolia and populus balsamifera. the binary vector pcambia-npro-long-luc containing the luciferase reporter gene was used to transform stem internode and axillary bud explants. putative transformants were regenerated on selection-free medium using our previously established in vitro regeneration method. explant type, genotype, effect of pre-culture, agrobacteriu ... | 2016 | 27014319 |
| using the β-glucosidase catalyzed reaction product glucose to improve the ionic liquid tolerance of β-glucosidases. | pretreating biomass with ionic liquids (il) increases enzyme accessibility and cellulose is typically recovered through precipitation with an anti-solvent. an industrially feasible pretreatment and hydrolysis process requires robust cellulases that are stable and active in the presence of either small amounts of ils co-precipitated with recovered cellulose or for saccharifications in the presence of il. β-glucosidase (bg) hydrolyzes cellobiose into two molecules of glucose (glc) and is the last ... | 2016 | 27006691 |
| groe chaperonins assisted functional expression of bacterial enzymes in saccharomyces cerevisiae. | rapid advances in the capabilities of reading and writing dna along with increasing understanding of microbial metabolism at the systems-level have paved an incredible path for metabolic engineering. despite these advances, post-translational tools facilitating functional expression of heterologous enzymes in model hosts have not been developed well. some bacterial enzymes, such as escherichia coli xylose isomerase (xi) and arabinose isomerase (ai) which are essential for utilizing cellulosic su ... | 2016 | 27003667 |
| production of a novel bioflocculant and its flocculation performance in aluminum removal. | a novel bioflocculant cbf with high flocculating activity, produced by mixed culture of rhizobium radiobacter f2 and bacillus sphaericus f6 from soil, was investigated with regard to its production and flocculation performance in al(iii) removal. the most preferred carbon source, nitrogen source and c/n ratio (w/w) for strains f2 and f6 to produce cbf were glucose, urea and 20, respectively. the optimal inoculum size for cbf production was 10 % (v/v). the optimal initial ph, culture temperature ... | 2016 | 27002778 |
| efficient construction of large genomic deletion in agrobacterium tumefaciens by combination of cre/loxp system and triple recombineering. | in order to develop an efficient system for deleting genomic segment in agrobacterium tumefaciens to analyze gene functions and construct marker gene-free recombinant strains, a cre recombinase expression plasmid was constructed by placing its encoding gene under the control of ptet promoter and cloning into the plasmid replicable in both a. tumefaciens and e. coli. triple recombineering was applied to efficiently construct integrative vectors which were used to introduce loxp sites and selectio ... | 2016 | 26742770 |
| effects of a petunia scaffold/matrix attachment region on copy number dependency and stability of transgene expression in nicotiana tabacum. | transgenes in genetically modified plants are often not reliably expressed during development or in subsequent generations. transcriptional gene silencing (tgs) as well as post-transcriptional gene silencing (ptgs) have been shown to occur in transgenic plants depending on integration pattern, copy number and integration site. in an effort to reduce position effects, to prevent read-through transcription and to provide a more accessible chromatin structure, a p35s-ß-glucuronidase (p35s-gus) tran ... | 2016 | 26732611 |
| transformation of lactuca sativa l. with rol c gene results in increased antioxidant potential and enhanced analgesic, anti-inflammatory and antidepressant activities in vivo. | lettuce is an important edible crop which possesses various medicinal properties. in this study lactuca sativa l. (cv grand rapids) was transformed by agrobacterium-mediated transformation with rol c gene. transgene integration and expression was confirmed through pcr and semiquantitative rt-pcr. the transformed extracts were evaluated for their in vitro antioxidant and in vivo analgesic, anti-inflammatory and antidepressant activities in rats. the transformed plants showed 53-98 % increase in t ... | 2016 | 28330287 |
| parameters influencing agrobacterium-mediated transformation system in safflower genotypes aks-207 and pkv pink. | shoot regeneration in safflower (carthamus tinctorius 'aks 207' and 'pkv pink') genetically transformed using agrobacterium was used for assessing various constraints to the efficiency of transformation including infection period, virulence induction medium, co-cultivation period, bacterial titre, selection regime, and the natural phenolic compound acetosyringone. transformation frequency was promising with 8-10-day-old cotyledonary leaf explants. therefore, explants of that age cultured on agro ... | 2016 | 28330253 |
| identification and characterization of endophytic bacteria isolated from in vitro cultures of peach and pear rootstocks. | endophytes are microorganisms which live symbiotically with almost all varieties of plant and in turn helping the plant in a number of ways. instead of satisfactory surface sterilization approaches, repeatedly occurring bacterial growth on in vitro rootstock cultures of peach and pear was identified and isolated as endophytic bacteria in our present study. five different isolates from peach rootstocks were molecularly identified by 16s rrna gene sequencing as brevundimonas diminuta, leifsonia sh ... | 2016 | 28330195 |
| a simple and efficient agrobacterium tumefaciens-mediated plant transformation of brassica rapa ssp. pekinensis. | the present study aims to investigate the numerous factors influencing agrobacterium tumefaciens-mediated genetic transformation of chinese cabbage (brassica rapa ssp. pekinensis). factors affecting transformation efficiency, such as age of explants, agrobacterium concentration, and effect of acetosyringone, pre-cultivation, infection and co-cultivation time of agrobacterium were examined. the pre-cultured hypocotyls from young seedlings prior to exposure to agrobacterium showed higher shoot reg ... | 2016 | 28330158 |
| exploration of nifh gene through soil metagenomes of the western indian himalayas. | this group has previously highlighted the prevalence of csp genes from cold himalayan environments. however, this study has explored the uncultured diazotrophs from metagenomes of western indian himalayas. the metagenomic nifh gene clone library was constructed from the temperate, subtropical and tarai soils of western himalaya, india followed by polymerase chain reaction (pcr) amplification. after preliminary screening, selected clones were sequenced. in silico analysis of the clones was done, ... | 2016 | 28330093 |
| the plant gaba signaling downregulates horizontal transfer of the agrobacterium tumefaciens virulence plasmid. | in the tumor-inducing (ti) agrobacterium tumefaciens, quorum sensing activates the horizontal transfer of the virulent ti plasmid. in pure culture, this process can be impaired by the a. tumefaciens blcc lactonase, whose expression is induced by gamma-aminobutyrate (gaba). it was therefore hypothesized that host gaba content might modulate quorum sensing and virulence gene dissemination during a. tumefaciens infection. we examined gaba metabolism and transport in arabidopsis thaliana tumors comb ... | 2016 | 26714842 |
| transformation of medicinal plants using agrobacterium tumefaciens. | for many years attempts are made to develop efficient methods for transformation of medicinal plants via agrobacterium tumefaciens. it is a soil bacteria which possess a natural ability to infect plants in places of injures which results in arise of cancerous growths (crown gall). this is possible thanks a transfer of fragment of ti plasmid into plant cells and stable integration with a plant genome. efficiency of medicinal plant transformation depends on many factors for example: agrobacterium ... | 2016 | 28026825 |
| the use of stable and unstable green fluorescent proteins for studies in two bacterial models: agrobacterium tumefaciens and xanthomonas campestris pv. campestris. | fluorescent proteins have been used to track plant pathogens to understand their host interactions. to be useful, the transgenic pathogens must present similar behaviour than the wild-type isolates. herein, a gfp marker was used to transform two plant pathogenic bacteria, agrobacterium and xanthomonas, to localize and track the bacteria during infection. the transgenic bacteria were evaluated to determine whether they showed the same fitness than the wild-type strains or whether the expression o ... | 2016 | 27995281 |
| characterization and functional analysis of calmodulin and calmodulin-like genes in fragaria vesca. | calcium is a universal messenger that is involved in the modulation of diverse developmental and adaptive processes in response to various stimuli. calmodulin (cam) and calmodulin-like (cml) proteins are major calcium sensors in all eukaryotes, and they have been extensively investigated for many years in plants and animals. however, little is known about cams and cmls in woodland strawberry (fragaria vesca). in this study, we performed a genome-wide analysis of the strawberry genome and identif ... | 2016 | 27990153 |
| non-host resistance induced by the xanthomonas effector xopq is widespread within the genus nicotiana and functionally depends on eds1. | most gram-negative plant pathogenic bacteria translocate effector proteins (t3es) directly into plant cells via a conserved type iii secretion system, which is essential for pathogenicity in susceptible plants. in resistant plants, recognition of some t3es is mediated by corresponding resistance (r) genes or r proteins and induces effector triggered immunity (eti) that often results in programmed cell death reactions. the identification of r genes and understanding their evolution/distribution b ... | 2016 | 27965697 |
| plant-phytopathogen interactions: bacterial responses to environmental and plant stimuli. | plant pathogenic bacteria attack numerous agricultural crops, causing devastating effects on plant productivity and yield. they survive in diverse environments, both in plants, as pathogens, and also outside their hosts as saprophytes. hence, they are confronted with numerous changing environmental parameters. during infection, plant pathogens have to deal with stressful conditions, such as acidic, oxidative and osmotic stresses; anaerobiosis; plant defenses; and contact with antimicrobial compo ... | 2016 | 27878915 |
| expression in grasses of multiple transgenes for degradation of munitions compounds on live-fire training ranges. | the deposition of toxic munitions compounds, such as hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine (rdx), on soils around targets in live-fire training ranges is an important source of groundwater contamination. plants take up rdx but do not significantly degrade it. reported here is the transformation of two perennial grass species, switchgrass (panicum virgatum) and creeping bentgrass (agrostis stolonifera), with the genes for degradation of rdx. these species possess a number of agronomic trait ... | 2016 | 27862819 |