Publications

phytoremediation of mercury in pristine and crude oil contaminated soils: contributions of rhizobacteria and their host plants to mercury removal.	the rhizospheric soils of three tested legume crops: broad beans (vicia faba), beans (phaseolus vulgaris) and pea (pisum sativum), and two nonlegume crops: cucumber (cucumis sativus) and tomato, (lycopersicon esculentum) contained considerable numbers (the magnitude of 10(5)g(-1) soil) of bacteria with the combined potential for hydrocarbon-utilization and mercury-resistance. sequencing of the 16s rrna coding genes of rhizobacteria associated with broad beans revealed that they were affiliated t ...	2010	20833430
genetic transformation of lignin degrading fungi facilitated by agrobacterium tumefaciens.	white-rot fungi are primarily the major degraders of lignin, a major obstacle for commercial exploitation of plant byproducts to produce bioethanol and other industrially important products. however, to improve their efficacy for lignin degradation, it has become necessary to genetically modify these organisms using appropriate vectors. agrobacterium tumefaciens, a soil phytopathogenic bacterium, generally transforms plants by delivering a portion of the resident ti- plasmid, the t-dna (transfer ...	2010	20836896
comparing symbiotic efficiency between swollen versus nonswollen rhizobial bacteroids.	symbiotic rhizobia differentiate physiologically and morphologically into nitrogen-fixing bacteroids inside legume host nodules. the differentiation is apparently terminal in some legume species, such as peas (pisum sativum) and peanuts (arachis hypogaea), likely due to extreme cell swelling induced by the host. in other legume species, such as beans (phaseolus vulgaris) and cowpeas (vigna unguiculata), differentiation into bacteroids, which are similar in size and shape to free-living rhizobia, ...	2010	20837702
the auxin-signaling pathway is required for the lateral root response of arabidopsis to the rhizobacterium phyllobacterium brassicacearum.	plant root development is highly responsive both to changes in nitrate availability and beneficial microorganisms in the rhizosphere. we previously showed that phyllobacterium brassicacearum stm196, a plant growth-promoting rhizobacteria strain isolated from rapeseed roots, alleviates the inhibition exerted by high nitrate supply on lateral root growth. since soil-borne bacteria can produce iaa and since this plant hormone may be implicated in the high nitrate-dependent control of lateral root d ...	2010	20844890
isolation and characterization of temperature and alkaline stable bioflocculant from agrobacterium sp. m-503.	a bacterium isolated from activated sludge of propylene epoxide wastewater was identified as agrobacterium sp. m-503. it was confirmed to produce bioflocculant with excellent flocculation activity. the yield of the bioflocculant reached 14.9 g/l in batch cultivation with a carbon source conversion of 74.5%. this bioflocculant was temperature and alkaline stable, retaining almost all flocculation activity after being treated at 121°c for 20 minutes or at ph 12.0. it consisted of neutral sugar, ur ...	2010	20849992
genome sequence of the polymyxin-producing plant-probiotic rhizobacterium paenibacillus polymyxa e681.	paenibacillus polymyxa e681, a spore-forming, low-g+c, gram-positive bacterium isolated from the rhizosphere of winter barley grown in south korea, has great potential for agricultural applications due to its ability to promote plant growth and suppress plant diseases. here we present the complete genome sequence of p. polymyxa e681. its 5.4-mb genome encodes functions specialized to the plant-associated lifestyle and characteristics that are beneficial to plants, such as the production of a pla ...	2010	20851896
characterization of liposomes formed by lipopolysaccharides from burkholderia cenocepacia, burkholderia multivorans and agrobacterium tumefaciens: from the molecular structure to the aggregate architecture.	the microstructure of liposomes formed by the lipopolysaccharides (lps) derived from burkholderia cenocepacia et-12 type strain lmg 16656, burkholderia multivorans strain c1576 and agrobacterium tumefaciens strain tt111 has been investigated by a combined experimental strategy, including dynamic light scattering (dls), small-angle neutron scattering (sans) and electron paramagnetic resonance (epr). the results highlight that the lps molecular structure determines, through a complex interplay of ...	2010	20852798
development of an efficient gene targeting system in colletotrichum higginsianum using a non-homologous end-joining mutant and agrobacterium tumefaciens-mediated gene transfer.	the hemibiotrophic ascomycete colletotrichum higginsianum is the casual agent of anthracnose disease of cruciferous plants. high efficiency transformation by agrobacterium tumefaciens-mediated gene transfer has been established for this fungus. however, targeted gene mutagenesis through homologous recombination rarely occurs in c. higginsianum. we have identified and disrupted the c. higginsianum homologue of the human ku70 gene, chku70, which encodes a protein that plays a role in non-homologou ...	2010	20853009
mature seed-derived callus of the model indica rice variety kasalath is highly competent in agrobacterium-mediated transformation.	we previously established an efficient agrobacterium-mediated transformation system using primary calli derived from mature seeds of the model japonica rice variety nipponbare. we expected that the shortened tissue culture period would reduce callus browning--a common problem with the indica transformation system during prolonged tissue culture in the undifferentiated state. in this study, we successfully applied our efficient transformation system to kasalath--a model variety of indica rice. th ...	2010	20853107
evidence of transfer by conjugation of type iv secretion system genes between bartonella species and rhizobium radiobacter in amoeba.	bartonella species cospeciate with mammals and live within erythrocytes. even in these specific niches, it has been recently suggested by bioinformatic analysis of full genome sequences that lateral gene transfer (lgt) may occur but this has never been demonstrated biologically. here we describe the sequence of the b. rattaustraliani (aust/nh4(t)) circular plasmid (pnh4) that encodes the tra cluster of the type iv secretion system (t4ss) and we eventually provide evidence that bartonella species ...	2010	20856925
the organophosphate-degrading enzyme from agrobacterium radiobacter displays mechanistic flexibility for catalysis.	the op (organophosphate)-degrading enzyme from agrobacterium radiobacter (opda) is a binuclear metallohydrolase able to degrade highly toxic op pesticides and nerve agents into less or non-toxic compounds. in the present study, the effect of metal ion substitutions and site-directed mutations on the catalytic properties of opda are investigated. the study shows the importance of both the metal ion composition and a hydrogen-bond network that connects the metal ion centre with the substrate-bindi ...	2010	20868365
agrobacterium tumefaciens mediated fused egfp-hph gene expression under the control of gpd promoter in pleurotus ostreatus.	a transformation system for the basidiomycete pleurotus ostreatus was established using agrobacterium-mediated infection. following p. ostreatus glyceraldehyde-3-phosphate dehydrogenase gene analysis, its promoter region including two introns was used as cis-regulatory element to drive expression of enhanced green fluorescent protein (egfp). as a selection marker, the hygromycin phosphotransferase (hph) gene cassette was used in the binary vector ppeh. mycelia without pretreatment were found to ...	2010	20869218
introduction of large dna inserts into the barley pathogenic fungus, ustilago hordei, via recombined binary bac vectors and agrobacterium-mediated transformation.	genetic transformation of organisms with large genome fragments containing complete genes, with regulatory elements or clusters of genes, can contribute to the functional analysis of such genes. however, large inserts, such as those found on bacterial artificial chromosome (bac) clones, are often not easy to transfer. we exploited an existing technique to convert bac clones, containing genomic dna fragments from the barley-covered smut fungus ustilago hordei to binary bacs (bibacs) to make them ...	2010	20936474
tolerance to agricultural pesticides of strains belonging to four genera of rhizobiaceae.	in order to determine their tolerance to pesticides, 122 strains of rhizobia isolated from different geographical regions, and belonging to the genera rhizobium, mesorhizobium, sinorhizobium and bradyrhizobium were tested against eight herbicides, four fungicides and five insecticides. sensitivity to the pesticides was measured by using the filter paper disk method at four concentrations, 0.45, 4.5, 45 and 450 μg per disk. when the pesticides were used at 0.45 μg per disk, a concentration simila ...	2010	20936564
nematicidal activity of a nonpathogenic biocontrol bacterium, pseudomonas chlororaphis o6.	bacterial culture filtrates of an aggressive rhizobacterium, pseudomonas chlororaphis o6, displayed strong nematicidal activity. the nematicidal activity of p. chlororaphis o6 was markedly reduced in the gacs mutant of p. chlororaphis o6 grown in the presence of glycine, but no reduction of nematicidal activity in the gacs mutant was noted in the absence of glycine. the results of bioassay with p. chlororaphis o6 mutants showed that phenazine and pyrrolnitrin production was not a major factor, b ...	2010	20963417
ljcyc genes constitute floral dorsoventral asymmetry in lotus japonicus.	previous study shows that ljcyc2, a cyc-like tcp (tb1, cyc and pcfs) gene in the model legume, lotus japonicus, is involved in dorsal petal development, which together with the other two homologous genes, ljcyc1 and ljcyc3, belongs to an ljcyc gene cluster. in this report, we modified the transformation system in l. japonicus, and constructed different rnai transgenes to target different ljcyc genes. the expression of three endogenous ljcyc genes was specifically suppressed by different specific ...	2010	20977654
carboxymethylated cyclosophoraose as a novel chiral additive for the stereoisomeric separation of some flavonoids by capillary electrophoresis.	a carboxymethylated cyclosophoraose (cm-cys) was synthesized by the chemical modification of neutral cys, which was isolated from rhizobium trifolii ta-1. cm-cys was successfully applied as a novel chiral selector for the separation of some flavonoids including catechin, 3,5,7,3',4'-pentahydroxyflavanone, hesperidin, hesperetin, isosakuranetin, naringenin, naringin, and eriodictyol. the effects of ph, chiral additive concentration, and temperature on resolution and migration time were also studi ...	2010	20850110
genotypic and phenotypic diversity of rhizobia isolated from lathyrus japonicus indigenous to japan.	sixty-one rhizobial strains from lathyrus japonicus nodules growing on the seashore in japan were characterized and compared to two strains from canada. the pcr-based method was used to identify test strains with novel taxonomic markers that were designed to discriminate between all known lathyrus rhizobia. three genomic groups (i, ii, and iii) were finally identified using rapd, rflp, and phylogenetic analyses. strains in genomic group i (related to rhizobium leguminosarum) were divided into tw ...	2010	20851546
agrobacterium tumefaciens mediated transformation of chiv gene to trichoderma harzianum.	as a soil-borne filamentous fungus, trichoderma harzianum exhibits biological control properties because it parasitizes a large variety of phytopathogenic fungi. in this study, the vectors pbi121 and pcambia1301 and cloning vector puc18 were used to successfully construct expression vector pca-gchiv for filamentous fungi transformation mediated by agrobacterium tumefaciens.the chiv gene was successfully transferred into the biocontrol fungus t. harzianum with an efficiency of 90-110 transformant ...	2010	20936373
complete genome sequence of paenibacillus polymyxa sc2, a strain of plant growth-promoting rhizobacterium with broad-spectrum antimicrobial activity.	paenibacillus polymyxa sc2 is an important plant growth-promoting rhizobacterium (pgpr). here, we report the complete genome sequence of p. polymyxa sc2. multiple sets of functional genes have been found in the genome. as far as we know, this is the first complete genome sequence of paenibacillus polymyxa.	2010	21037012
a mesorhizobium lipopolysaccharide (lps) specific lectin (crl) from the roots of nodulating host plant, cicer arietinum.	a 30 kda rabbit erythrocyte agglutinating glycoprotein isolated and characterized from the roots of cicer arietinum and designated as cicer root lectin (crl). hemagglutination activity of crl is strongly inhibited by cell surface lps of nodulating cicer specific rhizobium. crl agglutinates mesorhizobial cells and not escherichia coli or yeast cells. it binds to immobilized lps of cicer specific rhizobium only. the primary structure of crl as predicted by peptide mass fingerprinting by maldi-tof ...	2010	21055439
the nod factor-independent symbiotic signaling pathway: development of agrobacterium rhizogenes-mediated transformation for the legume aeschynomene indica.	the nitrogen-fixing symbiosis between aeschynomene indica and photosynthetic bradyrhizobia is the only legume-rhizobium association described to date that does not require lipochito-oligosaccharide nod factors (nf). to assist in deciphering the molecular basis of this nf-independent interaction, we have developed a protocol for agrobacterium rhizogenes-mediated transformation of a. indica. the cotransformation frequency (79%), the nodulation efficiency of transgenic roots (90%), and the expressi ...	2010	21039272
high-level expression of human interferon alpha-2b in transgenic carrot (daucus carota l.) plants.	in this study, we report the obtaining of carrot plants expressing human interferon alpha-2b via agrobacterium-mediated transformation using two vector constructs containing the sequence coding for interferon gene fused with nicotiana plumbagenifolia calreticulin apoplast targeting signal driven by 35s camv promoter and root-specific mll promoter. the human interferon alpha-2b gene was correctly translated in carrot plants according to western blot analysis. the recombinant protein exhibited ant ...	2010	21046110
production of human interferon alfa 2b in plants of nicotiana excelsior by agrobacterium-mediated transient expression.	human interferon alpha2b gene was transiently expressed in nicotiana excelsior plants. fusion with n. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 x 10(3) iu/g fresh weight (fw) with an average of 2.1 +/- 0.8 x 10(3) iu/g fw. it proves that n. excelsior is a suitable host for agrobacterium-mediated transient expression of genes encoding physiologically active h ...	2010	21058531
presence of opportunistic bacteria (rhizobium spp.) with potential for molecular misdiagnosis among canine and feline clinical samples.	rhizobium radiobacter was detected in 12 of 187 dogs and 2 of 100 cats using a polymerase chain reaction (pcr) assay formerly designed for the rickettsia genus. although pcr primers used for pathogenic infectious agents are specifically assessed to avoid cross-amplification, this retrospective study highlights the importance of sequencing to avoid molecular misdiagnosis.	2010	21037894
characterization of a two-component signal transduction system that controls arsenite oxidation in the chemolithoautotroph nt-26.	nt-26 is a chemolithoautotrophic arsenite oxidizer. understanding the mechanisms of arsenite signalling, tolerance and oxidation by nt-26 will have significant implications for its use in bioremediation and arsenite sensing. we have identified the histidine kinase (aros) and the cognate response regulator (aror) involved in the arsenite-dependent transcriptional regulation of the arsenite oxidase aroba operon. aros contains a single periplasmic sensory domain that is linked through transmembrane ...	2010	21039781
molecular analysis of a homogentisate phytyltransferase gene from lactuca sativa l.	tocochromanols, usually known as vitamin e, play a crucial role in human and animal nutrition. the enzyme homogentisate phytyltransferase (hpt) performs the first committed step of the vitamin e biosynthetic pathway. the full-length cdna encoding hpt was isolated from lactuca sativa l. by rapid amplification of cdna ends (race). the cdna, designated as lshpt, was 1,670 bp long containing an open reading frame (orf) of 1,185 bp which encoded a protein of 395 amino acids. sequence analysis indicat ...	2010	20872075
[the nadph oxidase activity of pea seedling roots in rhizobial infection depending on abiotic and biotic factors].	the changes in nadph activity was studied in the roots of 3-4-day-old etiolated pea (cultivar aksaiskii usatyi) seedlings depending on plant inoculation with rhizobium leguminosarum bv. viceae (strain ciam 1026), adverse environmental factors (low temperature and high dose of a mineral nitrogen fertilizer), chemical substances (sodium nitroprusside and methyl viologen, or paraquat), and a biotic factor--the bacterium escherichia coli (strain xl-1 blue). it was demonstrated that all exogenous fac ...	2010	20873175
bio-encapsulation of microbial cells for targeted agricultural delivery.	biofertilizers, namely rhizobium and biocontrol agents such as pseudomonas and trichoderma have been well established in the field of agricultural practices for many decades. nevertheless, research is still going on in the field of inoculant production to find methods to improve advanced formulation and application in fields. conventionally used solid and liquid formulations encompass several problems with respect to the low viability of microorganisms during storage and field application. there ...	2010	20879835
a new method to determine initial viability of entrapped cells using fluorescent nucleic acid staining.	entrapped bacterial cells are widely used in several biotechnological applications. cell entrapment procedures are known to affect the viability of bacterial cells. to determine the effect of entrapment procedures on viability of bacterial cells, dissolution of the entrapment matrices using chelating agents or heat is required immediately after the entrapment is completed. chelating agents and heat applied in the matrix dissolution reduce cell viability and in turn hinder accurate quantification ...	2010	20933406
enhanced v-atpase activity contributes to the improved salt tolerance of transgenic tobacco plants overexpressing vacuolar na(+)/h (+) antiporter atnhx1.	atnhx1, a vacuolar na(+)/h(+) antiporter gene from arabidopsis thaliana, was introduced into tobacco genome via agrobacterium tumefaciens-mediated transformation to evaluate the role of vacuolar energy providers in plants salt stress response. compared to the wild-type plants, over-expression of atnhx1 increased salt tolerance in the transgenic tobacco plants, allowing higher germination rates of seeds and successful seedling establishment in the presence of toxic concentrations of nacl. more im ...	2010	20882319
substrate-dependent auxin production by rhizobium phaseoli improves the growth and yield of vigna radiata l. under salt stress conditions.	rhizobium phaseoli strains were isolated from the mung bean nodules, and, the most salt tolerant and high auxin producing rhizobial isolate n20 was evaluated in the presence and absence of l-tryptophan (l-trp) for improving growth and yield of mung bean under saline conditions in a pot experiment. mung bean seeds were inoculated with peat-based inoculum and np fertilizers were applied at 30-60 kg ha-1, respectively. results revealed that imposition of salinity reduced the growth and yield of mun ...	2010	20890093
the impact of environmental contamination with antibiotics on levels of resistance in soil bacteria.	this study examined the effects of tetracycline and streptomycin on microorganisms in three different soil habitats: forest soil, agricultural soil, and compost. these antibiotics are commonly used in both medical and veterinary therapy as well as in the production of plant biomass and until quite recently, the production of animal biomass. microcosms were used as model systems in which the number of microorganisms in environments containing different amounts of antibiotics was analyzed. the min ...	2010	21043273
functional characterization of replication and stability factors of an incompatibility group p-1 plasmid from xylella fastidiosa.	xylella fastidiosa strain riv11 harbors a 25-kbp plasmid (pxf-riv11) belonging to the incp-1 incompatibility group. replication and stability factors of pxf-riv11 were identified and used to construct plasmids able to replicate in x. fastidiosa and escherichia coli. replication in x. fastidiosa required a 1.4-kbp region from pxf-riv11 containing a replication initiation gene (trfa) and the adjacent origin of dna replication (oriv). constructs containing trfa and oriv from pveis01, a related incp ...	2010	20935126
metal inducible activity of the oil palm metallothionein-like gene promoter (mt3-a) in prokaryotes.	reporter gene activity under the regulation of the oil palm metallothionein-like gene, mt3-a promoter was assessed in prokaryotes. vector constructs containing mt3-a promoter with (w1mt3-a) and without (w2mt3-a) five prime untranslated region (5'-utr) fused to ß-glucuronidase (gus) gene in pcambia 1304 vector were produced. 5'-rapid amplification of cdna ends (race) using mrna isolated from escherichia coli and agrobacterium tumefaciens harboring w1mt3-a confirmed that fusion transcripts of mt3- ...	2010	21044862
plant defense pathways subverted by agrobacterium for genetic transformation.	the soil phytopathogen agrobacterium has the unique ability to introduce single-stranded transferred dna (t-dna) from its tumor-inducing (ti) plasmid into the host cell in a process known as horizontal gene transfer. following its entry into the host cell cytoplasm, the t-dna associates with the bacterial virulence (vir) e2 protein, also exported from agrobacterium, creating the t-dna nucleoprotein complex (t-complex), which is then translocated into the nucleus where the dna is integrated into ...	2010	20890133
complex prokaryotic genome structure: rapid evolution of chromosome ii.	although many bacteria with two chromosomes have been sequenced, the roles of such complex genome structuring are still unclear. to uncover levels of chromosome i (ci) and chromosome ii (cii) sequence divergence, mauve 2.2.0 was used to align the ci- and cii-specific sequences of bacteria with complex genome structuring in two sets of comparisons: the first set was conducted among the ci and cii of bacterial strains of the same species, while the second set was conducted among the ci and cii of ...	2010	20924417
r gene-controlled host specificity in the legume-rhizobia symbiosis.	leguminous plants can enter into root nodule symbioses with nitrogen-fixing soil bacteria known as rhizobia. an intriguing but still poorly understood property of the symbiosis is its host specificity, which is controlled at multiple levels involving both rhizobial and host genes. it is widely believed that the host specificity is determined by specific recognition of bacterially derived nod factors by the cognate host receptor(s). here we describe the positional cloning of two soybean genes rj2 ...	2010	20937853
the extracellular proteome of rhizobium etli ce3 in exponential and stationary growth phase.	abstract:	2010	20942974
tsukamurella tyrosinosolvens and rhizobium radiobacter sepsis presenting with septic pulmonary emboli.	clin microbiol infect abstract: septic pulmonary embolism (spe) is an uncommon, but life-threatening event that is usually associated with extrapulmonary infections. we report the first case of bilateral spe secondary to a central venous catheter-related bloodstream infection involving pathogens commonly considered environmental contaminants: tsukamurella tyrosinosolvens and rhizobium radiobacter. empirical levofloxacin treatment was confirmed by in vitro susceptibility data and produced prompt ...	2010	20946410
swarm intelligence in plant roots.		2010	20952090
investigating agrobacterium-mediated transformation of verticillium albo-atrum on plant surfaces.	agrobacterium tumefaciens has long been known to transform plant tissue in nature as part of its infection process. this natural mechanism has been utilised over the last few decades in laboratories world wide to genetically manipulate many species of plants. more recently this technology has been successfully applied to non-plant organisms in the laboratory, including fungi, where the plant wound hormone acetosyringone, an inducer of transformation, is supplied exogenously. in the natural envir ...	2010	21060684
agrobacterium tumefaciens-mediated transformation for investigation of somatic recombination in the fungal pathogen armillaria mellea.	armillaria root disease is one of the most damaging timber and fruit tree diseases in the world. despite its economic importance, many basic questions about the biology of the causal fungi, armillaria spp., are unanswered. for example, armillaria undergoes matings between diploid and haploid mycelia, which can result in a recombinant diploid without meiosis. evidence of such somatic recombination in natural populations suggests that this reproductive mode may affect the pathogen's ecology. inves ...	2010	20952653
bidirectionalization of a methyl jasmonate-inducible plant promoter.	a unidirectional promoter can be transformed into a bidirectional module by artificial methods. here we report the bidirectionalization of the methyl jasmonate (meja)-inducible ptdrl02 promoter derived from poplar [(populus tomentosa × p. bolleana) × p. tomentosa] in planta. construction of the bidirectional ptdrl02 promoter (designated as mptdrl02) was rapidly achieved by introducing a minimal 35s promoter in the opposite orientation to the 5' end of ptdrl02. β-glucuronidase (gus) and green flu ...	2010	20953667
diverse rhizobia associated with sophora alopecuroides grown in different regions of loess plateau in china.	a total of seventy-five symbiotic bacterial strains isolated from root nodules of wild sophora alopecuroides grown in different regions of china's loess plateau were characterized. based on the combined rflp patterns, thirty-five genotypes were defined among the rhizobia and they were classified into nine genomic species, including mesorhizobium alhagi and m. gobiense as the main groups, as well as agrobacterium tumefaciens, m. amorphae, phyllobacterium trifolii, rhizobium giardinii, r. indigofe ...	2010	20965680
the nodulation of alfalfa by the acid-tolerant rhizobium sp. strain lpu83 does not require sulfated forms of lipochitooligosaccharide nodulation signals.	the induction of root nodules by the majority of rhizobia has a strict requirement for the secretion of symbiosis-specific lipochitooligosaccharides (nodulation factors [nfs]). the nature of the chemical substitution on the nfs depends on the particular rhizobium and contributes to the host specificity imparted by the nfs. we present here a description of the genetic organization of the nod gene cluster and the characterization of the chemical structure of the nfs associated with the broad-host- ...	2010	20971905
regeneration of transgenic plants by agrobacterium-mediated transformation of somatic embryos of juvenile and mature quercus robur.	a protocol was developed for genetic transformation of somatic embryos derived from juvenile and mature quercus robur trees. optimal transformation conditions were evaluated on the basis of the results of transient gus expression assays with five oak embryogenic lines and a strain of agrobacterium tumefaciens (eha105) harbouring a p35sgusint plasmid containing a nptii and a uida (gus) genes. for stable transformation, embryo clumps at globular/torpedo stages (4-10 mg) were inoculated with eha105 ...	2010	20972795
[effects of different factors on transformed hairy root in gynostemm apentaphyllum].	to induce hairy roots of gynostemm apentaphyllum by agrobacterium rhizogenes strains.	2010	21434425
hairy root cultures for secondary metabolites production.	hairy roots (hrs) are differentiated cultures of transformed roots generated by the infection of wounded higher plants with agrobacterium rhizogenes. this pathogen causes the hr disease leading to the neoplastic growth of roots that are characterized by high growth rate in hormone free media and genetic stability. hrs produce the same phytochemicals pattern of the corresponding wild type organ. high stability and productivity features allow the exploitation of hrs as valuable biotechnological to ...	2010	21520711
the determinants of the actinorhizal symbiosis.	the actinorhizal symbiosis is a major contributor to the global nitrogen budget, playing a dominant role in ecological successions following disturbances. the mechanisms involved are still poorly known but there emerges the vision that on the plant side, the kinases that transmit the symbiotic signal are conserved with those involved in the transmission of the rhizobium nod signal in legumes. however, on the microbial side, complementation with frankia dna of rhizobium nod mutants failed to perm ...	2010	21576879
from defense to symbiosis: limited alterations in the kinase domain of lysm receptor-like kinases are crucial for evolution of legume-rhizobium symbiosis.	nitrogen-fixing symbiosis between legumes and rhizobia is initiated by the recognition of rhizobial nod factors (nfs) by host plants. nfs are diversely modified derivatives of chitin oligosaccharide, a fungal elicitor that induces defense responses in plants. recent evidence has shown that both nfs and chitin elicitors are recognized by structurally related lysm receptor kinases. transcriptome analyses of lotus japonicus roots indicated that nfs not only activate symbiosis genes but also transie ...	2010	21223383
effect of co-inoculation with mycorrhiza and rhizobia on the nodule trehalose content of different bean genotypes.	studies on rhizobium-legume symbiosis show that trehalose content in nodules under drought stress correlates positively with an increase in plant tolerance to this stress. fewer reports describe trehalose accumulation in mycorrhiza where, in contrast with rhizobia, there is no flux of carbohydrates from the microsymbiont to the plant. however, the trehalose dynamics in the mycorrhiza-rhizobium-legume tripartite symbiosis is unknown. the present study explores the role of this tripartite symbiosi ...	2010	21253462
[application of rhizobia-legume symbiosis for remediation of heavy-metal contaminated soils].	heavy-metal pollution in soil is a major threat to human health and the entire ecosystem. legumes and their associated rhizobial microorganisms are important for the biogeochemical cycles in agriculture and natural ecosystems. legume-rhizobia symbiosis is very important in restoration of heavy-metal contaminated soils because the fixation of atmospheric nitrogen by legume-rhizobium symbiosis can increase the accumulation of nitrogen and organic matter. we reviewed the importance of remediation o ...	2010	21268885
complete genome sequence of rhizobium leguminosarum bv. trifolii strain wsm1325, an effective microsymbiont of annual mediterranean clovers.	rhizobium leguminosarum bv trifolii is a soil-inhabiting bacterium that has the capacity to be an effective nitrogen fixing microsymbiont of a diverse range of annual trifolium (clover) species. strain wsm1325 is an aerobic, motile, non-spore forming, gram-negative rod isolated from root nodules collected in 1993 from the greek island of serifos. wsm1325 is produced commercially in australia as an inoculant for a broad range of annual clovers of mediterranean origin due to its superior attribute ...	2010	21304718
[cotransformation of aspen and birch with three t-dna regions from two different replicons in one agrobacterium tumefaciens strain].	the cointegration rate into the aspen and birch genomes of foreign genes from a binary vector and a disarmed ti plasmid pcbe21 carried by the same agrobacterium tumefaciens strain was studied. the cotransformation rate for the genes within the ti plasmid varied from 30 to 100%; while the transformation rate for the gene from t(l) region was twofold higher as compared with the t(r) region. on the average, the gene transfer from all three t-dnas was recorded in 10.9% of the transgenic lines. for t ...	2010	21261057
effect of different strains of agrobacterium rhizogenes and nature of explants on plumbago indica hairy root culture with special emphasis on root biomass and plumbagin production.	the aim of the present study was to determine the effect of three strains of agrobacterium rhizogenes (atcc 15834, a4 and lba 9402) and the nature of explants (leaf and stem) on hairy root induction, growth and plumbagin production in plumbago indica. the first appearance of hairy roots, the transformation frequency, dry root biomass and plumbagin accumulation were found to be maximum in hairy roots induced in leaf explants infected with a. rhizogenes atcc 15834 as compared with the other two ba ...	2010	21299119
complete genome sequence of rhizobium leguminosarum bv trifolii strain wsm2304, an effective microsymbiont of the south american clover trifolium polymorphum.	rhizobium leguminosarum bv trifolii is the effective nitrogen fixing microsymbiont of a diverse range of annual and perennial trifolium (clover) species. strain wsm2304 is an aerobic, motile, non-spore forming, gram-negative rod, isolated from trifolium polymorphum in uruguay in 1998. this microsymbiont predominated in the perennial grasslands of glencoe research station, in uruguay, to competitively nodulate its host, and fix atmospheric nitrogen. here we describe the basic features of wsm2304, ...	2010	21304679
genetic dissection of rhizobium-induced infection and nodule organogenesis in pea based on enod12a and enod5 expression analysis.	in legumes, perception of rhizobial lipochitooligosacharide-based molecules (nod factors) and subsequent signal transduction triggers transcription of plant symbiosis-specific genes (early nodulins). we present genetic dissection of nod factor-controlled processes in pisum sativum using two early nodulin genes psenod12a and psenod5, that are differentially up-regulated during symbiosis. a novel set of non-nodulating pea mutants in fourteen loci was examined, among which seven loci are not descri ...	2010	21309975
effects of cell lysis treatments on the yield of coenzyme q10 following agrobacterium tumefaciens fermentation.	the yield of coq₁₀, an intracellular product extracted from agrobacterium tumefaciens cells is dependent on the effectiveness of cell lysis post fermentation. various cell lysis approaches are investigated, including ultrasound, repetitive freezing/thawing, grinding and acid-heat treatment. the acid-heat combination using hydrochloric acid is found the most effective in releasing coq₁₀, followed by lactic, sulfuric, phosphoric and oxalic acids. the most significant processing parameters, namely ...	2010	21339135
positive-selection and ligation-independent cloning vectors for large scale in planta expression for plant functional genomics.	transient expression is an easy, rapid and powerful technique for producing proteins of interest in plants. recombinational cloning is highly efficient but has disadvantages, including complicated, time consuming cloning procedures and expensive enzymes for large-scale gene cloning. to overcome these limitations, we developed new ligation-independent cloning (lic) vectors derived from binary vectors including tobacco mosaic virus (pjl-trbo), potato virus x (pgr106) and the pbi121 vector-based pm ...	2010	21340673
brucellosis: review on the recent trends in pathogenicity and laboratory diagnosis.	brucellosis is a zoonotic infection transmitted from animals to humans by the ingestion of infected food products, direct contact with an infected animal or inhalation of aerosols. the last method is remarkably efficient given the relatively low concentration of organisms (10 - 100 bacteria) needed to establish infection in humans, and has brought renewed attention to this old disease. brucella is a facultative intracellular pathogen that has the ability to survive and multiply in the phagocytes ...	2010	21346896
agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in medicinal fungus cordyceps militaris.	cordyceps militaris is an insect-born fungus with various biological and pharmacological activities. the mutant library of c. militaris was constructed by improved agrobacterium tumefaciens-mediated transformation (atmt), for the ultimate identification of genes involved in isolate degeneration during fruiting body production. successful transformation of c. militaris jm4 by a. tumefaciens agl-1 carrying vector patmt1 was performed, with efficiency in the range of 30-600 transformants per 1×10(5 ...	2010	21354533
[activity of main enzymes of ammonium assimilation in bradyrhizobium japonicum under the influence of plant flavonoid inductors].	the activity of key enzymes of ammonium assimilation in cell-free extracts of soybean rhizobia characterized with different effectiveness in symbiosis, has been researched. it has been found out that at highly efficient strain bradyrhizobium japonicum ukm b-6035 the most active links in the system of ammonium assimilation reactions are glutamate synthase (glutamate-oxoglutarate-aminotransferase, gogat) and glutamate dehydrogenase (gdh). whereas the most active links at the ineffective strain b. ...	2010	21381313
a c subunit of the plant nuclear factor nf-y required for rhizobial infection and nodule development affects partner selection in the common bean-rhizobium etli symbiosis.	legume plants are able to interact symbiotically with soil bacteria to form nitrogen-fixing root nodules. although specific recognition between rhizobia and legume species has been extensively characterized, plant molecular determinants that govern the preferential colonization by different strains within a single rhizobium species have received little attention. we found that the c subunit of the heterotrimeric nuclear factor nf-y from common bean (phaseolus vulgaris) nf-yc1 plays a key role in ...	2010	21139064
influence of rhizobium meliloti on phytoremediation of polycyclic aromatic hydrocarbons by alfalfa in an aged contaminated soil.	microbe-assisted phytoremediation is emerging as one of the most effective means by which plants and their associated rhizosphere microbes degrade organic contaminants in soils. a pot study was conducted to examine the effects of inoculation with rhizobium meliloti on phytoremediation by alfalfa grown for 90 days in an agricultural soil contaminated with weathered polycyclic aromatic hydrocarbons (pahs). planting with uninoculated alfalfa (p) and alfalfa inoculated with r. meliloti (pr) signific ...	2010	21177027
production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker.	the use of antibiotic or herbicide resistant genes as selection markers for production of transgenic plants and their continuous presence in the final transgenics has been a serious problem for their public acceptance and commercialization. mat (multi-auto-transformation) vector system has been one of the different strategies to excise the selection marker gene and produce marker-free transgenic plants. in the present study, ipt (isopentenyl transferase) gene was used as a selection marker gene. ...	2010	21184230
biochemical characteristics of the novel haloalkane dehalogenase data, isolated from the plant pathogen agrobacterium tumefaciens c58.	we report the biochemical characterization of a novel haloalkane dehalogenase, data, isolated from the plant pathogen agrobacterium tumefaciens c58. data possesses a peculiar pair of halide-stabilizing residues, asn-tyr, which have not been reported to play this role in other known haloalkane dehalogenases. data has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chi ...	2010	21193677
genetic and phenotypic diversity of plant growth promoting rhizobacteria isolated from sugarcane plants growing in pakistan.	bacteria were isolated from roots of sugarcane varieties grown in the fields of punjab. they were identified by using api20e/ne bacterial identification kits and from sequences of 16s rrna and amplicons of the cpn60 gene. the majority of bacteria were found to belong to the genera of enterobacter, pseudomonas, and klebsiella, but members of genera azospirillum, rhizobium, rahnella, delftia, caulobacter, pannonibacter, xanthomonas, and stenotrophomonas were also found. the community, however, was ...	2010	21193815
stable transformation of mesembryanthemum crystallinum (l.) with agrobacterium rhizogenes harboring the green fluorescent protein targeted to the endoplasmic reticulum.	stable transformation of mesembryanthemum crystallinum l. (common ice plant) with a green fluorescent protein (gfp) construct targeted to the endoplasmic reticulum was obtained. seven and fourteen days after germination seedlings were infected with agrobacterium rhizogenes strain arqua1 either by direct coating of the cut radicles with bacteria growing on solid medium or by immersion of the cut surface in bacterial suspension at different optical densities. both methods of infection resulted in ...	2010	21195506
agroinfiltration as a technique for rapid assays for evaluating candidate insect resistance transgenes in plants.	functional analysis of candidate transgenes for insect resistance in stably transformed plants is a time-consuming task that can take months to achieve in even the fastest of plant models. in this study, a rapid screening technique is described, which employs candidate transgene transient expression using agroinfiltration in nicotiana benthamiana combined with a simple insect bioassay. using this system the known insecticidal protein cry1ac is demonstrated to effectively control helicoverpa zea. ...	2010	21140154
[culture of hairy roots of panax japonicus and ginsenoside re synthesis].	to establish the induction method and culture system of hairy roots of panax japonicus, and determine ginsenoside re contents.	2010	21141482
ovarian hydrobursitis in female camels (camelus dromedaries): biochemical, bacterial and protozoal evaluation.	the aim of this study was to evaluate female camels affected with ovarian hydrobursitis (n = 31) for hematological and biochemical findings and for bacterial and protozoal infections. blood samples were obtained and surgical ablation of the affected bursa was performed. bursal fluid, follicular fluid, and serum were subjected to hormonal and biochemical analyses. bursal fluids were cultured and colonies were identified using biomérieux vitek two compact system. passive haemagglutination test was ...	2010	21144565
plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils.	technogenic activities (industrial-plastic, textiles, microelectronics, wood preservatives; mining-mine refuse, tailings, smelting; agrochemicals-chemical fertilizers, farm yard manure, pesticides; aerosols-pyrometallurgical and automobile exhausts; biosolids-sewage sludge, domestic waste; fly ash-coal combustion products) are the primary sources of heavy metal contamination and pollution in the environment in addition to geogenic sources. during the last two decades, bioremediation has emerged ...	2010	21147211
lysm-type mycorrhizal receptor recruited for rhizobium symbiosis in nonlegume parasponia.	rhizobium-root nodule symbiosis is generally considered to be unique for legumes. however, there is one exception, and that is parasponia. in this nonlegume, the rhizobial nodule symbiosis evolved independently and is, as in legumes, induced by rhizobium nod factors. we used parasponia andersonii to identify genetic constraints underlying evolution of nod factor signaling. part of the signaling cascade, downstream of nod factor perception, has been recruited from the more-ancient arbuscular endo ...	2010	21205637
rhizobium pusense sp. nov., isolated from rhizosphere of chickpea (cicer arietinum l).	a bacterial strain nrcpb10t was isolated from rhizosphere soil of chickpea (cicer arietinum l), in pusa, new delhi, india.16s rrna gene sequence of the strain nrcpb10t showed the highest similarity 98.9% with rhizobium radiobacter icmp 5785t followed by rhizobium larrymoorei icmp 15237t (97.7%) and rhizobium rubi lmg 17935t (97.4%). phylogenetic analysis of strain nrcpb10t using housekeeping genes reca and atpd revealed their distinct position differing from other recognized rhizobium species. d ...	2010	21148676
a glutamic acid-rich protein identified in verticillium dahliae from an insertional mutagenesis affects microsclerotial formation and pathogenicity.	verticillium dahliae kleb. is a phytopathogenic fungus that causes wilt disease in a wide range of crops, including cotton. the life cycle of v. dahliae includes three vegetative phases: parasitic, saprophytic and dormant. the dormant microsclerotia are the primary infectious propagules, which germinate when they are stimulated by root exudates. in this study, we report the first application of agrobacterium tumefaciens-mediated transformation (atmt) for construction of insertional mutants from ...	2010	21151869
expression of baculovirus anti-apoptotic genes p35 and op-iap in cotton (gossypium hirsutum l.) enhances tolerance to verticillium wilt.	programmed cell death plays an important role in mediating plant adaptive responses to the environment such as the invasion of pathogens. verticillium wilt, caused by the necrotrophic pathogen verticillium dahliae, is a serious vascular disease responsible for great economic losses to cotton, but the molecular mechanisms of verticillium disease and effective, safe methods of resistance to verticillium wilt remain unexplored.	2010	21151969
nicotiana benthamiana as a production platform for artemisinin precursors.	production of pharmaceuticals in plants provides an alternative for chemical synthesis, fermentation or natural sources. nicotiana benthamiana is deployed at commercial scale for production of therapeutic proteins. here the potential of this plant is explored for rapid production of precursors of artemisinin, a sesquiterpenoid compound that is used for malaria treatment.	2010	21151979
development of a transgenic hairy root system in jute (corchorus capsularis l.) with gusa reporter gene through agrobacterium rhizogenes mediated co-transformation.	transgenic hairy root system is important in several recalcitrant plants, where agrobacterium tumefaciens-mediated plant transformation and generation of transgenic plants are problematic. jute (corchorus spp.), the major fibre crop in indian subcontinent, is one of those recalcitrant plants where in vitro tissue culture has provided a little success, and hence, agrobacterium-mediated genetic transformation remains to be a challenging proposition in this crop. in the present work, a system of tr ...	2010	21153028
molecular insights into plant cell proliferation disturbance by agrobacterium protein 6b.	the agrobacterium ti plasmid (t-dna) 6b proteins interact with many different host proteins implicated in plant cell proliferation. here, we show that arabidopsis plants overexpressing 6b display microrna (mirna) deficiency by directly targeting serrate and ago1 via a specific loop fragment (residues 40-55). in addition, we report the crystal structures of agrobacterium tumefaciens ak6b at 2.1 å, agrobacterium vitis ab6b at 1.65 å, and arabidopsis adp ribosylation factor (arf) at 1.8 å. the 6b s ...	2010	21156810
biofilm-forming bacteria with varying tolerance to peracetic acid from a paper machine.	biofilms cause runnability problems in paper machines and are therefore controlled with biocides. peracetic acid is usually effective in preventing bulky biofilms. this study investigated the microbiological status of a paper machine where low concentrations (≤15 ppm active ingredient) of peracetic acid had been used for several years. the paper machine contained a low amount of biofilms. biofilm-forming bacteria from this environment were isolated and characterized by 16s rrna gene sequencing, ...	2010	21161323
an efficient foxtail mosaic virus vector system with reduced environmental risk.	plant viral vectors offer high-yield expression of pharmaceutical and commercially important proteins with a minimum of cost and preparation time. the use of agrobacterium tumefaciens has been introduced to deliver the viral vector as a transgene to each plant cell via a simple, nonsterile infiltration technique called "agroinoculation". with agroinoculation, a full length, systemically moving virus is no longer necessary for excellent protein yield, since the viral transgene is transcribed and ...	2010	21162736
a novel antifungal pseudomonas fluorescens isolated from potato soils in greenland.	a rhizobacterium with high antifungal activity was isolated from a potato field at inneruulalik, south greenland. phylogenetic analysis based on multi locus sequence typing showed that the bacterium was affiliated with strains of pseudomonas fluorescens. the bacterium, denoted as pseudomonas fluorescens in5, inhibited in vitro a broad range of phytopathogenic fungi, and the antifungal activity increased with decreasing temperature. microcosm experiments demonstrated that p. fluorescens in5 prote ...	2010	21165740
influence of arbuscular mycorrhiza and rhizobium on phytoremediation by alfalfa of an agricultural soil contaminated with weathered pcbs: a field study.	a field experiment was conducted to study the effects of inoculation with the arbuscular mycorrhizal fungus glomus caledonium and/or rhizobium meliloti on phytoremediation of an agricultural soil contaminated with weathered pcbs by alfalfa grown for 180 days. planting alfalfa (p), alfalfa inoculated with g. caledonium (p + am), alfalfa inoculated with r. meliloti (p + r), and alfalfa co-inoculated with r. meliloti and g. caledonium (p+am+r) decreased significantly initial soil pcb concentrations ...	2010	21166292
only half the transcriptomic differences between resistant genetically modified and conventional rice are associated with the transgene.	besides the intended effects that give a genetically modified (gm) plant the desired trait, unintended differences between gm and non-gm comparable plants may also occur. profiling technologies allow their identification, and a number of examples demonstrating that unintended effects are limited and diverse have recently been reported. both from the food safety aspect and for research purposes, it is important to discern unintended changes produced by the transgene and its expression from those ...	2010	21040388
an efficient protocol for genetic transformation of platycodon grandiflorum with agrobacterium rhizogenes.	the balloon flower (platycodon grandiflorum) is a popular traditional medicinal plant used in korea to treat conditions such as bronchitis, asthma, tuberculosis, diabetes, and inflammatory diseases. recently, immunopharmacological research identified triterpenoid and saponin as important active compounds in p. grandiflorum. to study and extract these compounds and other metabolites from p. grandiflorum, a technique was developed for producing hairy root cultures, which are a reliable source of p ...	2010	21052843
whole genome co-expression analysis of soybean cytochrome p450 genes identifies nodulation-specific p450 monooxygenases.	cytochrome p450 monooxygenases (p450s) catalyze oxidation of various substrates using oxygen and nad(p)h. plant p450s are involved in the biosynthesis of primary and secondary metabolites performing diverse biological functions. the recent availability of the soybean genome sequence allows us to identify and analyze soybean putative p450s at a genome scale. co-expression analysis using an available soybean microarray and illumina sequencing data provides clues for functional annotation of these ...	2010	21062474
rhizobium leguminosarum bv. trifolii rosr is required for interaction with clover, biofilm formation and adaptation to the environment.	rhizobium leguminosarum bv. trifolii is a symbiotic nitrogen-fixing bacterium that elicits nodules on roots of host plants trifolium spp. bacterial surface polysaccharides are crucial for establishment of a successful symbiosis with legumes that form indeterminate-type nodules, such as trifolium, pisum, vicia, and medicago spp. and aid the bacterium in withstanding osmotic and other environmental stresses. recently, the r. leguminosarum bv. trifolii rosr regulatory protein which controls exopoly ...	2010	21070666
origin and incidence of 2-methoxy-3,5-dimethylpyrazine, a compound with a "fungal" and "corky" aroma found in cork stoppers and oak chips in contact with wines.	this study identifies a previously isolated bacterium as rhizobium excellensis, a new species of proteobacteria able to form a large quantity of 2-methoxy-3,5-dimethylpyrazine (mdmp). r. excellensis actively synthesizes mdmp from l-alanine and l-leucine and, to a lesser extent, from l-phenylalanine and l-valine. mdmp is a volatile, strong-smelling substance detected in wines with cork stoppers that have an unpleasant "corky", "herbaceous" (potato, green hazelnut), or "dusty" odor that is very di ...	2010	21058737
integrating carbon-halogen bond formation into medicinal plant metabolism.	halogenation, which was once considered a rare occurrence in nature, has now been observed in many natural product biosynthetic pathways. however, only a small fraction of halogenated compounds have been isolated from terrestrial plants. given the impact that halogenation can have on the biological activity of natural products, we reasoned that the introduction of halides into medicinal plant metabolism would provide the opportunity to rationally bioengineer a broad variety of novel plant produc ...	2010	21048708
effect of transgenic rhizobacteria overexpressing citrobacter braakii appa on phytate-p availability to mung bean plants.	rhizosphere microorganisms possessing phytase activity are considered important for rendering phytate-p available to plants. in the present study, citrobacter braakii phytase gene (appa) was over-expressed in rhizobacteria possessing plant growth promoting (pgp) traits for increasing their potential as bioinoculants. appa was cloned under the lac promoter in the broad host-range expression vector pbbr1mcs2. transformation of the recombinant construct pcbappa resulted in high constitutive phytase ...	2010	21124052
rhizobium vallis sp. nov., isolated from nodules of three leguminous species grown in yunnan province of china.	four bacterial strains isolated from root nodules of phaseolus vulgaris, mimosa pudica and indigofera spicata plants grown in yunnan province of china were identified as a lineage within the genus rhizobium according to the analysis of 16s rrna gene sequence, most similar with r. lusitanum p1-7(t) (99.1% sequence similarity) and r. rhizogenes iam13570(t) (99.0%). these strains also formed a distinctive group from the reference strains for defined rhizobium species in a polyphasic approach, inclu ...	2010	21131504
structural characterization of a group ii 2/2 hemoglobin from the plant pathogen agrobacterium tumefaciens.	within the 2/2 hemoglobin sub-family, no group ii 2/2hbs from proteobacteria have been so far studied. here we present the first structural characterization of a group ii 2/2hb from the soil and phytopathogenic bacterium agrobacterium tumefaciens (at-2/2hbo). the crystal structure of ferric at-2/2hbo (reported at 2.1å resolution) shows the location of specific/unique heme distal site residues (e.g., his(42)cd1, a residue distinctive of proteobacteria group ii 2/2hbs) that surround a heme-ligande ...	2010	21070893
colutea arborescens is nodulated by diverse rhizobia in eastern morocco.	eighteen isolates of rhizobia isolated from root nodules of colutea arborescens (bladder senna) grown in different soils of the eastern area of morocco were characterized by phenotypic and genomic analyses. all the isolates characterized were fast growers. this is may be due to the isolation procedures used. the phenotypic, symbiotic and cultural characteristics analyzed allowed the description of a wide physiological diversity among tested isolates. the results obtained suggest that the phenoty ...	2010	21082309
most heat-tolerant rhizobia show high induction of major chaperone genes upon stress.	the aims of this study were to evaluate the temperature stress tolerance of chickpea rhizobia and to investigate whether tolerance is related to the species or the site of origin of the isolates. additionally, the molecular bases of temperature stress tolerance in rhizobia were investigated, by comparing the expression of chaperone genes dnakj and groesl in thermotolerant and thermosensitive isolates. tolerance to cold, heat and heat shock was evaluated for 53 mesorhizobia obtained from several ...	2010	21073488
broad-range antagonistic rhizobacteria pseudomonas fluorescens and serratia plymuthica suppress agrobacterium crown gall tumours on tomato plants.	to examine the biocontrol activity of broad-range antagonists serratia plymuthica ic1270, pseudomonas fluorescens q8r1-96 and p. fluorescens b-4117 against tumourigenic strains of agrobacterium tumefaciens and a. vitis.	2010	21091861
fluorescence of phytochrome adducts with synthetic locked chromophores.	we performed steady state fluorescence measurements with phytochromes agp1 and agp2 of agrobacterium tumefaciens and three mutants in which photoconversion is inhibited. these proteins were assembled with the natural chromophore biliverdin (bv), with phycoerythrobilin (peb), which lacks a double bond in the ring c-d-connecting methine bridge, and with synthetic bilin derivatives in which the ring c-d-connecting methine bridge is locked. all peb and locked chromophore adducts are photoinactive. a ...	2010	21071442
potassium chloride and rare earth elements improve plant growth and increase the frequency of the agrobacterium tumefaciens-mediated plant transformation.	plant transformation efficiency depends on the ability of the transgene to successfully interact with plant host factors. our previous work and the work of others showed that manipulation of the activity of host factors allows for increased frequency of transformation. recently we reported that exposure of tobacco plants to increased concentrations of ammonium nitrate increases the frequency of both homologous recombination and plant transgenesis. here we tested the influence of kcl and salts of ...	2010	21132499
mtnoa1/rif1 modulates medicago truncatula-sinorhizobium meliloti nodule development without affecting its nitric oxide content.	atnoa1/rif1 (formerly referred to as atnos1) has been shown to modulate nitric oxide (no) content in arabidopsis. as no generation in the legume-rhizobium symbiosis has been shown, the involvement of an atnoa1/rif1 orthologue from medicago truncatula (mtnoa1/rif1) during its symbiotic interaction with sinorhizobium meliloti has been studied. the expression of mtnoa1/rif1 appeared to occur mainly in nodule vascular bundles and the meristematic zone. using an rna interference strategy, transgenic ...	2010	21071678
agrobacterium tumefaciens-mediated transformation of the vegetative dikaryotic mycelium of the cultivated mushroom flammulina velutipes.	agrobacterium tumefaciens was used to transform the vegetative dikaryotic mycelium of flammulina velutipes using a hygromycin b resistance gene as selectable marker. the gene coding for urogen iii methyltransferase (cob) was introduced into f. velutipes dikaryotic cells. the resulting transformant cells generated a bright red fluorescence, indicating that cob is promising as a reporter gene in f. velutipes.	2010	21071861