Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| soybean [glycine max (l.) merr]. | in this chapter we describe an agrobacterium tumefaciens transformation method of soybean that utilizes mature half seeds and regeneration from the cotyledonary node region. this method results in fertile transformed soybean plants and transgenic seed in approximately 9 months. using mature half seeds as starting material has proven to be a reliable method that does not require additional wounding for infection to occur. we have continued to make improvements in the protocol, resulting in an eff ... | 2015 | 25300848 |
| cowpea [vigna unguiculata (l.) walp]. | agrobacterium tumefaciens-mediated transformation is an efficient method for incorporating genes and recovering stable transgenic plants in cowpea because this method offers several advantages such as the defined integration of transgenes, potentially low copy number, and preferential integration into transcriptional active regions of the chromosome. cotyledonary node explants of cowpea present an attractive target for t-dna delivery followed by regeneration of shoots via axillary proliferation ... | 2015 | 25300846 |
| wheat (triticum aestivum l.) transformation using immature embryos. | wheat may now be transformed very efficiently by agrobacterium tumefaciens. under the protocol hereby described, immature embryos of healthy plants of wheat cultivar fielder grown in a well-conditioned greenhouse were pretreated with centrifuging and cocultivated with a. tumefaciens. transgenic wheat plants were obtained routinely from between 40 and 90 % of the immature embryos, thus infected in our tests. all regenerants were normal in morphology and fully fertile. about half of the transforme ... | 2015 | 25300841 |
| rice, indica (oryza sativa l.). | indica varieties, which are generally recalcitrant to tissue culture and transformation, occupy 80 % of rice cultivation area in the world. therefore, transformation method for indica rice must be improved greatly so that global rice production would take full advantage of cutting-edge biotechnology. an efficient protocol for indica transformation mediated by agrobacterium tumefaciens is hereby described. immature embryos collected from plants in a greenhouse are cocultivated with a. tumefaciens ... | 2015 | 25300838 |
| maize (zea mays l.). | agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. in this chapter, we describe a detailed protocol for genetic transformation of the maize genotype hi ii. our starting plant material is immature embryos cocultivated with an agrobacterium strain carrying a standard binary vector. in addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the gree ... | 2015 | 25300834 |
| barley (hordeum vulgare l.) transformation using immature embryos. | barley is a major crop species, and also has become a genetic model for the small grain temperate cereals. a draft barley genome sequence has recently been completed, opening many opportunities for candidate gene isolation and functionality testing. thanks to the development of customizable endonucleases, also site-directed genome modification recently became feasible for higher plants, which marks the beginning of a new era of genetic engineering. the development of improved binary vectors and ... | 2015 | 25300832 |
| setaria viridis. | interest in plant model systems for genetic, biological, and functional genomics studies stems from advantages they provide in terms of fast generation time, small stature, and simple growth requirements. a model species would be especially advantageous for the studies of c4 photosynthetic grasses, which currently present practical challenges. these include long seed-to-seed generation times, and because of their large size at maturity they require large growing areas. one potential model of int ... | 2015 | 25300831 |
| brachypodium distachyon. | the small grass brachypodium distachyon has attributes that make it an excellent model for the development and improvement of cereal crops and bioenergy feedstocks. to realize the potential of this system, many tools have been developed (e.g., the complete genome sequence, a large collection of natural accessions, a high density genetic map, bac libraries, est sequences, microarrays, etc.). in this chapter, we describe a high-efficiency transformation system, an essential tool for a modern model ... | 2015 | 25300828 |
| a novel antibacterial peptide active against peach crown gall (agrobacterium tumefaciens) isolated from cyanide-tolerant actinomycetes g19. | an antimicrobial peptide was extracted from the antagonistic actinomycetes g19. it was designated as g19-f. by using maldi-tof mass spectrometry, the molecular weight of g19-f was determined. the primary structure of the antimicrobial peptide was determined using n-terminal sequencing and mass spectrometry. results showed that the peptide had eleven amino acids, with the sequence d-v-c-d-g-g-d-g-d-e-d, and a calculated molecular mass of 1,096 da. g19-f showed antimicrobial activity against peach ... | 2015 | 25358422 |
| novel compounds that enhance agrobacterium-mediated plant transformation by mitigating oxidative stress. | agrobacterium tumefaciens caused tissue browning leading to subsequent cell death in plant transformation and novel anti-oxidative compounds enhanced agrobacterium -mediated plant transformation by mitigating oxidative stress. browning and death of cells transformed with agrobacterium tumefaciens is a long-standing and high impact problem in plant transformation and the agricultural biotechnology industry, severely limiting the production of transgenic plants. using our tomato cv. microtom trans ... | 2015 | 25429877 |
| c-terminal domain of cagx is responsible for its interaction with cagt protein of helicobacter pylori type iv secretion system. | helicobacter pylori are the well known human pathogen associated with gastric cancer and peptic ulcer. pathogenesis is mainly due to the presence of 40 kb cagpai (cag pathogenicity island) region that encodes the type iv secretion system (tfss) consisting of a cytoplasmic part, a middle part/core complex (spans from inner membrane to outer membrane), and an outer membrane associated part. cagx and cagt are two important proteins of tfss that have homology with virb9 and virb7 of agrobacterium tu ... | 2015 | 25446105 |
| a novel reference plasmid for the qualitative detection of genetically modified rice in food and feed. | rice is one of the most important food crops in the world. genetically modified (gm) technology has been used in rice to confer herbicide tolerance and pathogen or insect resistance. china invests heavily in research on gm rice. by the end of 2014, at least 250 transgenic rice lines had been developed in china. to monitor the presence of gm rice in food and feed, we collected information on foreign elements from 250 transgenic rice lines and found 5 elements, including the agrobacterium tumefaci ... | 2015 | 26495318 |
| blocking single-stranded transferred dna conversion to double-stranded intermediates by overexpression of yeast dna replication factor a. | agrobacterium tumefaciens delivers its single-stranded transferred dna (t-strand) into the host cell nucleus, where it can be converted into double-stranded molecules. various studies have revealed that double-stranded transfer dna (t-dna) intermediates can serve as substrates by as yet uncharacterized integration machinery. nevertheless, the possibility that t-strands are themselves substrates for integration cannot be ruled out. we attempted to block the conversion of t-strands into double-str ... | 2015 | 25424309 |
| efficient transformation and expression of gfp gene in valsa mali var. mali. | valsa mali var. mali, the causal agent of valsa canker of apple, causes great loss of apple production in apple producing regions. the pathogenic mechanism of the pathogen has not been studied extensively, thus a suitable gene marker for pathogenic invasion analysis and a random insertion of t-dna for mutants are desirable. in this paper, we reported the construction of a binary vector pko1-hph containing a positive selective gene hygromycin phosphotransferase (hph), a reporter gene gfp conferri ... | 2015 | 25423905 |
| construction of infectious clones of tomato torrado virus and their delivery by agroinfiltration. | the first biologically active infectious clones of tomato torrado virus (totv) were generated and delivered into nicotiana benthamiana and solanum lycopersicum plants via agrobacterium tumefaciens. the engineered constructs consisted of pcr-amplified complementary dnas derived from the totv rna1 and rna2 components, individually inserted into an engineered pgreen binary vector between the camv 35s promoter and nopaline synthase terminator. these constructs were introduced into the plant hosts by ... | 2015 | 25416854 |
| tall fescue (festuca arundinacea schreb.). | tall fescue (festuca arundinacea schreb.) is the predominant cool-season perennial grass in the united states. it is widely used for both forage and turf purposes. this chapter describes a protocol that allows for the generation of a large number of transgenic tall fescue plants by agrobacterium tumefaciens-mediated transformation. embryogenic calli induced from caryopsis are used as explants for inoculation with a. tumefaciens. the agrobacterium strain used is eha105. hygromycin phosphotransfer ... | 2015 | 25416272 |
| poinsettia (euphorbia pulcherrima willd. ex klotzsch). | genetic engineering is an important tool for introducing desired genes into poinsettia (euphorbia pulcherrima willd. ex klotzsch). we describe in this chapter an agrobacterium tumefaciens-mediated transformation protocol for poinsettia. a detailed description of genetic transformation, antibiotic selection, subsequent regeneration via somatic embryogenesis, and rooting as well as molecular and morphological analyses is included. the methodology described here could facilitate the future engineer ... | 2015 | 25416270 |
| hemp (cannabis sativa l.). | hemp (cannabis sativa l.) suspension culture cells were transformed with agrobacterium tumefaciens strain eha101 carrying the binary plasmid pnov3635. the plasmid contains a phosphomannose isomerase (pmi) selectable marker gene. cells transformed with pmi are capable of metabolizing the selective agent mannose, whereas cells not expressing the gene are incapable of using the carbon source and will stop growing. callus masses proliferating on selection medium were screened for pmi expression usin ... | 2015 | 25416268 |
| sugarcane (saccharum spp. hybrids). | genetic transformation of sugarcane has a tremendous potential to complement traditional breeding in crop improvement and will likely transform sugarcane into a bio-factory for value-added products. we describe here agrobacterium tumefaciens-mediated transformation of sugarcane. embryogenic callus induced from immature leaf whorls was used as target for transformation with the hypervirulent agrobacterium strain agl1 carrying a constitutive nptii expression cassette in vector ppzp200. selection w ... | 2015 | 25416267 |
| peach (prunus persica l.). | until now, the application of genetic transformation techniques in peach has been limited by the difficulties in developing efficient regeneration and transformation protocols. here we describe an efficient regeneration protocol for the commercial micropropagation of gf677 rootstock (prunus persica × prunus amygdalus). the method is based on the production, via organogenesis, of meristematic bulk tissues characterized by a high competence for shoot regeneration. this protocol has also been used ... | 2015 | 25416260 |
| cherry. | agrobacterium tumefaciens-mediated transformation of sour chgahtvy (prunus cerasus l.) "montmorency" and sweet cherry rootstocks "gisela 6" and "gisela 7" (p. cerasus × p. canescens) is described. briefly, leaf explants from in vitro shoots are cocultivated with a. tumefaciens either directly (for "gisela 6" and "gisela 7") or after pretreatment (for "montmorency") on cocultivation medium; selection and regeneration of transformed shoots are carried out on selection medium containing 50 mg/l kan ... | 2015 | 25416255 |
| blueberry (vaccinium corymbosum l.). | vaccinium consists of approximately 450 species, of which highbush blueberry (vaccinium corymbosum) is one of the three major vaccinium fruit crops (i.e., blueberry, cranberry, and lingonberry) domesticated in the twentieth century. in blueberry the adventitious shoot regeneration using leaf explants has been the most desirable regeneration system to date; agrobacterium tumefaciens-mediated transformation is the major gene delivery method and effective selection has been reported using either th ... | 2015 | 25416254 |
| taro (colocasia esculenta (l.) schott). | genetic engineering of taro is an effective method to improve taro quality and the resistance to various diseases of taro. agrobacterium tumefaciens-mediated transformation of taro is more efficient than the particle bombardment transformation method based on current research. the development of a regeneration system starting from taro shoot tip explants could produce dasheen mosaic virus (dsmv)-free plantlets. highly regenerative calluses could be developed from dsmv-free, in vitro plantlets on ... | 2015 | 25416252 |
| carrot (daucus carota l.). | plants are susceptible to infection by a broad range of fungal pathogens. a range of proteins have been evaluated that can enhance tolerance to these pathogens by heterologous expression in transgenic carrot tissues. the protocols for carrot transformation with arabidopsis npr1 (non-expressor of pathogenesis-related proteins 1) are described in this chapter, using the herbicide resistance gene bar, which encodes phosphinothricin acetyltransferase, as a selectable marker. in this protocol, petiol ... | 2015 | 25416249 |
| sunflower (helianthus annuus l.). | sunflower (helianthus annuus l.) is still considered as a recalcitrant species to in vitro culture and transformation in spite of the publication of different protocols. here we describe a routine transformation system of this crop which requires mature ha89 genotype seeds and agrobacterium tumefaciens eha105 strain for gene delivery, being both easily available. selection of transformed shoots depends on root development in kanamycin-selective media, instead of shoot color, avoiding selection o ... | 2015 | 25416248 |
| jatropha (jatropha curcas l.). | the seed oil of jatropha (jatropha curcas l.) as a source of biodiesel fuel is gaining worldwide importance. commercial-scale exploration of jatropha has not succeeded due to low and unstable seed yield in semiarid lands unsuitable for the food production and infestation to diseases. genetic engineering is promising to improve various agronomic traits in jatropha and to understand the molecular functions of key jatropha genes for molecular breeding. we describe a protocol routinely followed in o ... | 2015 | 25416246 |
| genetic transformation of the tomato pathogen pyrenochaeta lycopersici allowed gene knockout using a split-marker approach. | pyrenochaeta lycopersici, as other soil-transmitted fungal pathogens, generally received little attention compared to the pathogens affecting the aerial parts of the plants, although causing stunt and important fruit yield reduction of agronomic relevant crops. the scope of this study was to develop a system allowing to investigate the functional role of p. lycopersici genes putatively involved in the corky root rot of tomato. a genetic transformation system based on a split-marker approach was ... | 2015 | 25413737 |
| membrane-binding mechanism of a bacterial phospholipid n-methyltransferase. | the membrane lipid phosphatidylcholine (pc) is crucial for stress adaptation and virulence of the plant pathogen agrobacterium tumefaciens. the phospholipid n-methyltransferase pmta catalyzes three successive methylations of phosphatidylethanolamine to yield pc. here, we asked how pmta is recruited to its site of action, the inner leaflet of the membrane. we found that the enzyme attaches to the membrane via electrostatic interactions with anionic lipids, which do not serve as substrate for pmta ... | 2015 | 25403021 |
| efficient generation of marker-free transgenic rice plants using an improved transposon-mediated transgene reintegration strategy. | marker-free transgenic plants can be developed through transposon-mediated transgene reintegration, which allows intact transgene insertion with defined boundaries and requires only a few primary transformants. in this study, we improved the selection strategy and validated that the maize (zea mays) activator/dissociation (ds) transposable element can be routinely used to generate marker-free transgenic plants. a ds-based gene of interest was linked to green fluorescent protein in transfer dna ( ... | 2015 | 25371551 |
| snare-rnai results in higher terpene emission from ectopically expressed caryophyllene synthase in nicotiana benthamiana. | plants produce numerous terpenes and much effort has been dedicated to the identification and characterization of the terpene biosynthetic genes. however, little is known about how terpenes are transported within the cell and from the cell into the apoplast. to investigate a putative role of vesicle fusion in this process, we used agrobacterium tumefaciens-mediated transient coexpression in nicotiana benthamiana of an mtvamp721e-rnai construct (vi) with either a caryophyllene synthase or a linal ... | 2015 | 25598143 |
| genomic analyses of metal resistance genes in three plant growth promoting bacteria of legume plants in northwest mine tailings, china. | to better understand the diversity of metal resistance genetic determinant from microbes that survived at metal tailings in northwest of china, a highly elevated level of heavy metal containing region, genomic analyses was conducted using genome sequence of three native metal-resistant plant growth promoting bacteria (pgpb). it shows that: mesorhizobium amorphae ccnwgs0123 contains metal transporters from p-type atpase, cdf (cation diffusion facilitator), hupe/urej and chr (chromate ion transpor ... | 2015 | 25597676 |
| chemical composition and antibacterial activity of methanolic extract and essential oil of iranian teucrium polium against some of phytobacteria. | the antibacterial activity of essential oil and methanolic extract of teucrium polium was determined against pseudomonas aeruginosa, pantoea agglomerans, brenneria nigrifluens, rhizobium radiobacter, rhizobium vitis, streptomyces scabies, ralstonia solanacearum, xanthomonas campestris and pectobacterium cartovorum by disc diffusion method. minimum inhibitory concentration and minimum bactericidal concentration were determined by using the serial dilution method. chemical composition of essential ... | 2015 | 25583240 |
| simple screening strategy with only water bath needed for the identification of insect-resistant genetically modified rice. | an informative, with simple instrument needed, rapid and easily updated strategy for the identification of insect-resistant genetically modified (gm) rice has been described. such strategy is based on a parallel series of loop-mediated isothermal amplification (lamp) reactions targeting the rice endogenous gene sucrose phosphate synthase (sps), the top two most frequently used genetic elements (agrobacterium tumefaciens nopaline synthase terminator (nos) and cauliflower mosaic virus 35s promoter ... | 2015 | 25582220 |
| arsenite oxidase also functions as an antimonite oxidase. | arsenic and antimony are toxic metalloids and are considered priority environmental pollutants by the u.s. environmental protection agency. significant advances have been made in understanding microbe-arsenic interactions and how they influence arsenic redox speciation in the environment. however, even the most basic features of how and why a microorganism detects and reacts to antimony remain poorly understood. previous work with agrobacterium tumefaciens strain 5a concluded that oxidation of a ... | 2015 | 25576601 |
| isolation and characterization of a secoiridoid derivative from two-phase olive waste (alperujo). | a secoiridoid derivative was isolated from the ethyl acetate extract of two-phase olive waste (alperujo). the structure of this compound was fully characterized as s-trans-(e)-3-(1-oxobut-2-en-2-yl)glutaric acid. the spectroscopic data, including one- and two-dimensional nuclear magnetic resonance, mass spectrometry, infrared analysis, and ultraviolet spectrum, were showed. the origin of this compound has not been previously studied, although it most likely results from the breakdown of the oleu ... | 2015 | 25574580 |
| cloning and expression of tnf related apoptosis inducing ligand in nicotiana tabacum. | molecular farming has been considered as a secure and economical approach for production of biopharmaceuticals. human tnf related apoptosis inducing ligand (trail) as a promising biopharmaceutical candidate has been produced in different expression hosts. however, little attention has been paid to molecular farming of the trail in spite of numerous advantages of plant expression systems. therefore, in this study the cytoplasmic production of the trail was tackled in nicotiana tabacum using agrob ... | 2015 | 25561925 |
| effect of agrobacterium strain and plasmid copy number on transformation frequency, event quality and usable event quality in an elite maize cultivar. | improving agrobacterium -mediated transformation frequency and event quality by increasing binary plasmid copy number and appropriate strain selection is reported in an elite maize cultivar. agrobacterium-mediated maize transformation is a well-established method for gene testing and for introducing useful traits in a commercial biotech product pipeline. to develop a highly efficient maize transformation system, we investigated the effect of two agrobacterium tumefaciens strains and three differ ... | 2015 | 25558819 |
| tobacco against ebola virus disease. | the ebola virus disease (evd), formerly known as a hemorrhagic fever and discovered in 1976, is dangerous, highly infectious disease with very high mortality. there are no licensed therapeutics against evd, although a range of medicines and therapies are currently being evaluated. during the 2014 ebola outbreak, an experimental drug named zmapp was administered on an emergency basis to seven patients of which five were recovered. currently, since february 2015, zmapp is tested in clinical trials ... | 2015 | 26946569 |
| effect of leaf incubation temperature profiles on agrobacterium tumefaciens-mediated transient expression. | agrobacterium tumefaciens-mediated transient expression is known to be highly dependent on incubation temperature. compared with early studies that were conducted at constant temperature, we examined the effect of variable leaf incubation temperature on transient expression. as a model system, synthetic endoglucanase (e1) and endoxylanase (xyn10a) genes were transiently expressed in detached whole sunflower leaves via vacuum infiltration for biofuel applications. we found that the kinetics of tr ... | 2015 | 25829353 |
| comparison and evaluation of two diagnostic methods for detection of npt ii and gus genes in nicotiana tabacum. | to diminish the time required for some diagnostic assays including polymerase chain reaction (pcr), loop-mediated isothermal amplification (lamp) and also a visual detection protocol on the basis of npt ii and gus genes in transgenic tobacco plants were used. agrobacterium tumefaciens-mediated transformation of nicotiana tabacum leaf discs was performed with plant transformation vector of pbi 121. from kanamycin-resistant plants selected by their antibiotic resistance, four plants were selected ... | 2015 | 25820356 |
| transient plant transformation mediated by agrobacterium tumefaciens: principles, methods and applications. | agrobacterium tumefaciens is widely used as a versatile tool for development of stably transformed model plants and crops. however, the development of agrobacterium based transient plant transformation methods attracted substantial attention in recent years. transient transformation methods offer several applications advancing stable transformations such as rapid and scalable recombinant protein production and in planta functional genomics studies. herein, we highlight agrobacterium and plant ge ... | 2015 | 25819757 |
| a fungal transcription factor gene is expressed in plants from its own promoter and improves drought tolerance. | a fungal gene encoding a transcription factor is expressed from its own promoter in arabidopsis phloem and improves drought tolerance by reducing transpiration and increasing osmotic potential. horizontal gene transfer from unrelated organisms has occurred in the course of plant evolution, suggesting that some foreign genes may be useful to plants. the cthsr1 gene, previously isolated from the halophytic yeast candida tropicalis, encodes a heat-shock transcription factor-related protein. cthsr1, ... | 2015 | 25809153 |
| nicotine-degrading microorganisms and their potential applications. | nicotine-degrading microorganisms (ndms) are a special microbial group which can use nicotine as the sole carbon and nitrogen source for growth. since the 1950s, the bioconversion of nicotine by microbes has received increasing attention, and several ndms have been identified, such as arthrobacter nicotinovorans, microsporum gypseum, pellicularia filamentosa jts-208, and pseudomonas sp. 41. in recent years, increasing numbers of ndms have been isolated and identified from tobacco plantation soil ... | 2015 | 25805341 |
| interaction of the agrobacterium tumefaciens virulence protein vird2 with histones. | agrobacterium tumefaciens is a gram-negative soil bacterium that genetically transforms plants and, under laboratory conditions, also transforms non-plant organisms, such as fungi and yeasts. during the transformation process a piece of ssdna (t-strand) is transferred into the host cells via a type iv secretion system. the vird2 relaxase protein, which is covalently attached at the 5' end of the t-strand through tyr29, mediates nuclear entry as it contains a nuclear localization sequence. how th ... | 2015 | 25505187 |
| an efficient plant regeneration and agrobacterium-mediated genetic transformation of tagetes erecta. | tagetes erecta, l. an asteraceous plant of industrial and medicinal value, contains important compounds like pyrethrins, thiophenes and lutein, possessing immense potential for insecticidal, nematicidal and nutraceutical activities. considering the importance and demand for these natural compounds, genetic manipulation of this crop for better productivity of secondary metabolites holds great significance. a rapid and reproducible direct regeneration and genetic transformation system is the prere ... | 2015 | 25504508 |
| insights into the polerovirus-plant interactome revealed by coimmunoprecipitation and mass spectrometry. | identification of host proteins interacting with the aphidborne potato leafroll virus (plrv) from the genus polerovirus, family luteoviridae, is a critical step toward understanding how plrv and related viruses infect plants. however, the tight spatial distribution of plrv to phloem tissues poses challenges. a polyclonal antibody raised against purified plrv virions was used to coimmunoprecipitate virus-host protein complexes from nicotiana benthamiana tissue inoculated with an infectious plrv c ... | 2015 | 25496593 |
| a rapid and stable agrobacterium-mediated transformation method of a medicinal plant chelone glabra l. | transformation approach is a useful tool for the study of gene function, the mechanism of molecular regulation, and increase usefulness of components by reverse genetic approach in plants. in this study, we developed a stable and rapid method for agrobacterium-mediated transformation of a medicinal plant chelone glabra l. using leaf explants. stable transformants were obtained using agrobacterium tumefaciens strains gv2260 and gv3101 that harbored the binary vector pbi121 and contained the neomy ... | 2015 | 25492686 |
| application of sonication in combination with vacuum infiltration enhances the agrobacterium-mediated genetic transformation in indian soybean cultivars. | soybean is a recalcitrant crop to agrobacterium-mediated genetic transformation. development of highly efficient, reproducible, and genotype-independent transformation protocol is highly desirable for soybean genetic improvement. hence, an improved agrobacterium-mediated genetic transformation protocol has been developed for cultivar pk 416 by evaluating various parameters including agrobacterium tumefaciens strains (lba4404, eha101, and eha105 harboring pcambia1304 plasmid), sonication duration ... | 2015 | 25480345 |
| engineering isoprenoid biosynthesis in artemisia annua l. for the production of taxadiene: a key intermediate of taxol. | taxadiene is the first committed precursor to paclitaxel, marketed as taxol, arguably the most important anticancer agent against ovarian and breast cancer. in taxus, taxadiene is directly synthesized from geranylgeranyl diphosphate (ggpp) that is the common precursor for diterpenoids and is found in most plants and microbes. in this study, artemisia annua l., a chinese medicinal herb that grows fast and is rich in terpenoids, was used as a genetic engineering host to produce taxadiene. the txs ... | 2015 | 25705665 |
| rhizobium metallidurans sp. nov., a symbiotic heavy metal resistant bacterium isolated from the anthyllis vulneraria zn-hyperaccumulator. | a gram-stain-negative, aerobic, rod-shaped, non-spore-forming bacterium (chimec512(t)) was isolated from 56 host seedlings of the hyperaccumulating anthyllis vulneraria legume, which was on an old zinc mining site at les avinières, saint-laurent-le-minier, gard, south of france. on the basis of 16s rrna gene sequence similarities, strain chimec512(t) was shown to belong to the genus rhizobium and to be most closely related to rhizobium endophyticum ccge 2052(t) (98.4%), rhizobium tibeticum ccbau ... | 2015 | 25701848 |
| a novel sterol regulatory element-binding protein gene (srea) identified in penicillium digitatum is required for prochloraz resistance, full virulence and erg11 (cyp51) regulation. | penicillium digitatum is the most destructive postharvest pathogen of citrus fruits, causing fruit decay and economic loss. additionally, control of the disease is further complicated by the emergence of drug-resistant strains due to the extensive use of triazole antifungal drugs. in this work, an orthologus gene encoding a putative sterol regulatory element-binding protein (srebp) was identified in the genome of p. digitatum and named srea. the putative srea protein contains a conserved domain ... | 2015 | 25699519 |
| optimization of chlorella vulgaris and bioflocculant-producing bacteria co-culture: enhancing microalgae harvesting and lipid content. | microalgae are a sustainable bioresource, and the biofuel they produce is widely considered to be an alternative to limited natural fuel resources. however, microalgae harvesting is a bottleneck in the development of technology. axenic chlorella vulgaris microalgae exhibit poor harvesting, as expressed by a flocculation efficiency of 0·2%. this work optimized the co-culture conditions of c. vulgaris and bioflocculant-producing bacteria in synthetic wastewater using response surface methodology ( ... | 2015 | 25693426 |
| aboveground insect infestation attenuates belowground agrobacterium-mediated genetic transformation. | agrobacterium tumefaciens causes crown gall disease. although agrobacterium can be popularly used for genetic engineering, the influence of aboveground insect infestation on agrobacterium induced gall formation has not been investigated. nicotiana benthamiana leaves were exposed to a sucking insect (whitefly) infestation and benzothiadiazole (bth) for 7 d, and these exposed plants were inoculated with a tumorigenic agrobacterium strain. we evaluated, both in planta and in vitro, how whitefly inf ... | 2015 | 25676198 |
| rhizobium pusense is the main human pathogen in the genus agrobacterium/rhizobium. | rhizobium pusense was recently described after isolation from the rhizosphere of chickpea. multilocus sequence-based analysis of clinical isolates identified as agrobacterium (rhizobium) radiobacter demonstrated that r. pusense is the main human pathogen within agrobacterium (rhizobium) spp. clinical microbiology of agrobacterium (rhizobium) should be considered in the light of recent taxonomic changes. | 2015 | 25669878 |
| a simple and efficient genetic transformation method of ganoderma weberianum. | in this study, the agrobacterium tumefaciens-mediated transformation method for ganderma weberianum has been established. driven by the cauliflower mosaic virus (camv) 35s promoter, the hygromycin phosphotransferase (hpt), β-glucuronidase (uida), and enhanced green fluorescent protein (egfp) genes have been efficiently expressed in transgenic mycelia and spores. the transformation system was composed of the growing mycelia, a. tumefaciens strain gv3101, and the expression vector pbi-h1, harborin ... | 2015 | 25655754 |
| comparative whole-genome analyses of selection marker-free rice-based cholera toxin b-subunit vaccine lines and wild-type lines. | we have developed a rice-based oral cholera vaccine named mucorice-ctb (cholera toxin b-subunit) by using an agrobacterium tumefaciens-mediated co-transformation system. to assess the genome-wide effects of this system on the rice genome, we compared the genomes of three selection marker-free mucorice-ctb lines with those of two wild-type rice lines (oryza sativa l. cv. nipponbare). mutation profiles of the transgenic and wild-type genomes were examined by next-generation sequencing (ngs). | 2015 | 25653106 |
| identification of pathogenicity-related genes in the rice pathogen ustilaginoidea virens through random insertional mutagenesis. | agrobacterium tumefaciens-mediated transformation (atmt) is becoming a popular effective system as an insertional mutagenesis tool in filamentous fungi. to gain more insight into the cellular and molecular mechanisms in the pathogenesis of ustilaginoidea virens, the causal agent of rice false smut disease, a t-dna insertion mutant library of u. virens was established using atmt. we optimized a range of conditions to improve the transformation efficiency. transformants were most effectively obtai ... | 2015 | 25636735 |
| progress in genetic engineering of peanut (arachis hypogaea l.)--a review. | peanut (arachis hypogaea l.) is a major species of the family, leguminosae, and economically important not only for vegetable oil but as a source of proteins, minerals and vitamins. it is widely grown in the semi-arid tropics and plays a role in the world agricultural economy. peanut production and productivity is constrained by several biotic (insect pests and diseases) and abiotic (drought, salinity, water logging and temperature aberrations) stresses, as a result of which crop experiences ser ... | 2015 | 25626474 |
| microbiological stability of canned tuna produced in italy and in non-european countries. | the results of a study on the microbiological stability of canned tuna produced by italian companies and similar canned products manufactured in countries outside europe are reported herein. the study involved 38 samples of canned tuna of various brands, of which 14 were produced by companies outside europe and 24 by italian companies. qualitative and quantitative microbiological tests were conducted for the following parameters: bacterial colony counts at 30°c, total coliforms, total enterobact ... | 2015 | 27800383 |
| rapid, high-throughput tracking of bacterial motility in 3d via phase-contrast holographic video microscopy. | tracking fast-swimming bacteria in three dimensions can be extremely challenging with current optical techniques and a microscopic approach that can rapidly acquire volumetric information is required. here, we introduce phase-contrast holographic video microscopy as a solution for the simultaneous tracking of multiple fast moving cells in three dimensions. this technique uses interference patterns formed between the scattered and the incident field to infer the three-dimensional (3d) position an ... | 2015 | 25762336 |
| quantitating plant microrna-mediated target repression using a dual-luciferase transient expression system. | microrna (mirna) mediated repression of target genes plays essential roles in a variety of functions in plants. an easy-to-use method that can effectively validate functional mirna-target interactions in plants thus is of particular interest. here, we describe an agrobacterium tumefaciens-mediated in vivo assay utilizing a dual-luciferase reporter system. with this method, the strength of mirna-mediated target repression can be quantified at both the mrna (via qrt-pcr) and protein (via dual-luci ... | 2015 | 25757778 |
| effects of tung oilseed fad2 and dgat2 genes on unsaturated fatty acid accumulation in rhodotorula glutinis and arabidopsis thaliana. | genetic engineering to produce valuable lipids containing unsaturated fatty acids (ufas) holds great promise for food and industrial applications. efforts to genetically modify plants to produce desirable ufas with single enzymes, however, have had modest success. the key enzymes fatty acid desaturase (fad) and diacylglycerol acyltransferase (dgat) are responsible for ufa biosynthesis (a push process) and assembling fatty acids into lipids (a pull process) in plants, respectively. to examine the ... | 2015 | 25754996 |
| molecular and phenotypic characterization of agrobacterium species from vineyards allows identification of typical agrobacterium vitis and atypical biovar 1 strains. | to molecularly and phenotypically characterize a selection of agrobacterium-like isolates from grapevine canes, crowns, soil and tumours in plants grown under cold conditions. | 2015 | 25752332 |
| characterization of the highly active fragment of glyceraldehyde-3-phosphate dehydrogenase gene promoter for recombinant protein expression in pleurotus ostreatus. | developing efficient native promoters is important for improving recombinant protein expression by fungal genetic engineering. the promoter region of glyceraldehyde-3-phosphate dehydrogenase gene in pleurotus ostreatus (pogpd) was isolated and optimized by upstream truncation. the activities of these promoters with different lengths were further confirmed by fluorescence, quantitative real-time pcr and western blot analysis. a truncated pogpd-p2 fragment (795 bp) drove enhanced green fluorescenc ... | 2015 | 25743073 |
| a functional genomics method for assaying gene function in phytopathogenic fungi through host-induced gene silencing mediated by agroinfiltration. | with the rapid growth of genomic information, there is an increasing demand for efficient analysis tools to study the function of predicted genes coded in genomes. agroinfiltration, the delivery of gene constructs into plant cells by agrobacterium tumefaciens infiltrated into leaves, is one such versatile, simple, and rapid technique that is increasingly used for transient gene expression assay in plants. in this chapter, we focus on the use of agroinfiltration as a functional genomics research ... | 2015 | 25740365 |
| a high-throughput rna interference (rnai)-based approach using hairy roots for the study of plant-rhizobia interactions. | legumes are major contributors to sustainable agriculture; their key feature is their ability to fix atmospheric nitrogen through symbiotic nitrogen fixation. legumes are often recalcitrant to regeneration and transformation by agrobacterium tumefaciens; however, a. rhizogenes-mediated root transformation and composite plant generation are rapid and convenient alternatives to study root biology, including root nodule symbiosis. rna interference (rnai), coupled with a. rhizogenes-mediated root tr ... | 2015 | 25740364 |
| transgene-induced gene silencing in plants. | rnai is the most important reverse genetics tool to trigger transgenic gene silencing, which is now applied widely to investigate gene function and also practically applied to enhance resistance to biotic and abiotic stress. recently, the most effective way to induce transgenic gene silencing is to introduce inverted repeat (ir) double-stranded rna (dsrna) or artificial microrna (amirna) instead of a transgenic sense or antisense strand of genes. the stable transgenic plants can be acquired thro ... | 2015 | 25740359 |
| against friend and foe: type 6 effectors in plant-associated bacteria. | bacterial secretion systems play critical roles in communication with neighboring bacteria and in the modulation of host immune responses via the secretion of small proteins called effectors. several secretion systems have been identified and these are denoted types i-vii. of these, the type vi secretion system (t6ss) and its effectors were only recently elucidated. most studies on the role and significance of the t6ss and its effectors have focused on human pathogens. in this review, type 6 eff ... | 2015 | 25732741 |
| spondylodiscitis due to rhizobium radiobacter. | 2015 | 25868625 | |
| [meristematic characteristics of tumors initiated by agrobacterium tumefaciens in pea plants]. | it is known that two key groups of plant hormones--auxins and cytokinins--play an important role in plant tumor development. the formation of agrobacterium-induced tumors results from the horizontal transfer of bacterial oncogenes involved in the biosynthesis of these hormones in the plant genome. the role of transcriptional factors in plant tumor development is poorly investigated. it can be assumed that tumor development associated with abnormal cell proliferation can be controlled by the same ... | 2015 | 25857193 |
| a ku70 null mutant improves gene targeting frequency in the fungal pathogen verticillium dahliae. | to overcome the challenges met with gene deletion in the plant pathogen verticillium dahliae, a mutant strain with impaired non-homologous end joining dna repair was generated to improve targeted gene replacement frequencies. a v. dahliae 991 δvdku70 null mutant strain was generated using agrobacterium tumefaciens-mediated transformation. despite having impaired non-homologous end joining dna repair function, the δvdku70 strain exhibited normal growth, reproduction capability, and pathogenicity ... | 2015 | 26475327 |
| atp-binding cassette (abc) transport system solute-binding protein-guided identification of novel d-altritol and galactitol catabolic pathways in agrobacterium tumefaciens c58. | innovations in the discovery of the functions of uncharacterized proteins/enzymes have become increasingly important as advances in sequencing technology flood protein databases with an exponentially growing number of open reading frames. this study documents one such innovation developed by the enzyme function initiative (efi; u54gm093342), the use of solute-binding proteins for transport systems to identify novel metabolic pathways. in a previous study, this strategy was applied to the tripart ... | 2015 | 26472925 |
| the agrobacterium tumefaciens virulence protein vire3 is a transcriptional activator of the f-box gene vbf. | during agrobacterium tumefaciens-mediated transformation of plant cells a part of the tumour-inducing plasmid, t-dna, is integrated into the host genome. in addition, a number of virulence proteins are translocated into the host cell. the virulence protein vire3 binds to the arabidopsis thaliana pbrp protein, a plant-specific general transcription factor of the tfiib family. to study a possible role for vire3 in transcriptional regulation, we stably expressed vire3 in a. thaliana under control o ... | 2015 | 26461850 |
| a novel phenolic compound, chloroxynil, improves agrobacterium-mediated transient transformation in lotus japonicus. | agrobacterium-mediated transformation is a commonly used method for plant genetic engineering. however, the limitations of agrobacterium host-plant interactions and the complexity of plant tissue culture often make the production of transgenic plants difficult. transformation efficiency in many legume species, including soybean and the common bean, has been reported to be quite low. to improve the transformation procedure in legumes, we screened for chemicals that increase the transformation eff ... | 2015 | 26176780 |
| heterologous dna uptake in cultured symbiodinium spp. aided by agrobacterium tumefaciens. | plant-targeted pcb302 plasmids containing sequences encoding gfp fusions with a microtubule-binding domain; gfp with the fimbrin actin-binding domain 2; and gfp with atrack1c from arabidopsis thaliana, all harbored in agrobacterium tumefaciens, were used to assay heterologous expression on three different clades of the photosynthetic dinoflagellate, symbiodinium. accessibility to the resistant cell wall and through the plasma membrane of these dinoflagellates was gained after brief but vigorous ... | 2015 | 26167858 |
| agrobacterium-mediated in planta genetic transformation of sugarcane setts. | an efficient, reproducible, and genotype-independent in planta transformation has been developed for sugarcane using setts as explant. traditional agrobacterium-mediated genetic transformation and in vitro regeneration of sugarcane is a complex and time-consuming process. development of an efficient agrobacterium-mediated transformation protocol, which can produce a large number of transgenic plants in short duration is advantageous. hence, in the present investigation, we developed a tissue cul ... | 2015 | 26152769 |
| agrobacterium tumefaciens gene transfer: how a plant pathogen hacks the nuclei of plant and nonplant organisms. | agrobacterium species are soilborne gram-negative bacteria exhibiting predominantly a saprophytic lifestyle. only a few of these species are capable of parasitic growth on plants, causing either hairy root or crown gall diseases. the core of the infection strategy of pathogenic agrobacteria is a genetic transformation of the host cell, via stable integration into the host genome of a dna fragment called t-dna. this genetic transformation results in oncogenic reprogramming of the host to the bene ... | 2015 | 26151736 |
| improving plant transformation using agrobacterium tumefaciens. | here, we report a quick and low-cost method to improve plant transformation using agrobacterium tumefaciens. this method involves the use of physical wounding, ultrasound, and an increase in exposure time to the bacteria. we show how the transformation rate increased from 0 to 14% when an ultrasound pulse of 10 s was used in conjunction with 96 h of bacterial exposure in eclipta alba explants. | 2015 | 26125878 |
| mild and severe cereal yellow dwarf viruses differ in silencing suppressor efficiency of the p0 protein. | viral pathogenicity has often been correlated to the expression of the viral encoded-rna silencing suppressor protein (ssp). the silencing suppressor activity of the p0 protein encoded by cereal yellow dwarf virus-rpv (cydv-rpv) and -rps (cydv-rps), two poleroviruses differing in their symptomatology was investigated. cydv-rpv displays milder symptoms in oat and wheat whereas cydv-rps is responsible for more severe disease. we showed that both p0 proteins (p0(cy-rpv) and p0(cy-rps)) were able to ... | 2015 | 26116275 |
| rapid mapping of insertional mutations to probe cell wall regulation in cryptococcus neoformans. | random insertional mutagenesis screens are important tools in microbial genetics studies. investigators in fungal systems have used the plant pathogen agrobacterium tumefaciens to create tagged, random mutations for genetic screens in their fungal species of interest through a unique process of trans-kingdom cellular transconjugation. however, identifying the locations of insertion has traditionally required tedious pcr-based methods, limiting the effective throughput of this system. we have dev ... | 2015 | 26112692 |
| corrigendum: induction, suppression and requirement of rna silencing pathways in virulent agrobacterium tumefaciens infections. | 2015 | 26111511 | |
| production of conjugated linoleic acid by heterologous expression of linoleic acid isomerase in oleaginous fungus mortierella alpina. | to increase the commercial value of oleaginous fungus mortierella alpina by incorporation of trans-10,cis-12 conjugated linoleic acid (cla) into the polyunsaturated fatty acids (pufas) of m. alpina via propionibacterium acnes isomerase (pai) conversion. | 2015 | 26109146 |
| optimization of in vitro regeneration and agrobacterium tumefaciens-mediated transformation with heat-resistant cdna in brassica oleracea subsp. italica cv. green marvel. | an efficient system for shoot regeneration and agrobacterium tumefaciens-mediated transformation of brassica oleracea cv. green marvel cultivar is described. this study focuses on developing shoot regeneration from hypocotyl explants of broccoli cv. green marvel using thidiazuron (tdz), zeatin, and kinetin, the optimization of factors affecting agrobacterium-mediated transformation of the hypocotyl explants with heat-resistant cdna, followed by the confirmation of transgenicity of the regenerant ... | 2015 | 25986972 |
| comparative performance of modified full-length and truncated bacillus thuringiensis-cry1ac genes in transgenic tomato. | bt-cry1ac gene has been reputedly effective against helicoverpa armigera a notorious lepidopteran pest. reports on the expression of full-length and truncated cry1ac genes in plants for effective resistance against helicoverpa sp. have been documented however, their performance is still ambiguous. moreover, the question remains to be addressed that truncation of 3' end of the native gene was documented and suggested for active insecticidal toxin production while the most successful transgenic ev ... | 2015 | 25977893 |
| efficient shoots regeneration and genetic transformation of bacopa monniera. | bacopa monniera is an important source of metabolites with pharmaceutical value. it has been regarded as a valuable medicinal plant and its entire commercial requirement is met from wild natural population. recently, metabolic engineering has emerged as an important solution for sustained supply of assured and quality raw material for the production of active principles. present report describes efficient in vitro multiplication and transformation method for genetic manipulation of this species. ... | 2015 | 25964718 |
| expression of enterovirus 71 virus-like particles in transgenic enoki (flammulina velutipes). | no commercial vaccines are currently available for enterovirus 71 (ev71) infection. oral virus-like particle (vlp) vaccines are regarded as a better choice for prevention from food-borne diseases compared with injected whole virus vaccines. unfortunately, the application of oral vlp vaccines produced from transgenic plants was limited due to the concerns of gene contamination. alternatively, using transgenic mushrooms retains the advantages of transgenic plants and tremendously reduce risks of g ... | 2015 | 25957149 |
| diversity and n-acyl-homoserine lactone production by gammaproteobacteria associated with avicennia marina rhizosphere of south indian mangroves. | the diversity of n-acyl-homoserine lactone (ahl)-producing rhizosphere bacterial community associated with avicennia marina in the mangrove ecosystems of south india was investigated. approximately 800 rhizobacteria were isolated from a. marina, and they were screened for the production of ahl using two biosensors, chromobacterium violaceum cv026 and agrobacterium tumefaciens ntl4 (pzlr4). among the total isolates screened, 7% of the rhizobacteria showed positive induction for ahl signals. the b ... | 2015 | 25956585 |
| gene overexpression and rna silencing tools for the genetic manipulation of the s-(+)-abscisic acid producing ascomycete botrytis cinerea. | the phytopathogenic ascomycete botrytis cinerea produces several secondary metabolites that have biotechnical significance and has been particularly used for s-(+)-abscisic acid production at the industrial scale. to manipulate the expression levels of specific secondary metabolite biosynthetic genes of b. cinerea with agrobacterium tumefaciens-mediated transformation system, two expression vectors (pcbh1 and pcbg1 with different selection markers) and one rna silencing vector, pcbsilent1, were ... | 2015 | 25955649 |
| characterization and comparative analysis of promoters from three plant pararetroviruses associated with dahlia (dahlia variabilis). | two distinct caulimoviruses, dahlia mosaic virus (dmv) and dahlia common mosaic virus (dcmv), and an endogenous plant pararetroviral sequence (dveprs, formerly known as dmv-d10) were reported from dahlia (dahlia spp). promoter elements from these dahlia-associated pararetroviruses were identified and characterized. the tata box, the caat box, the transcription start site, the polyadenylation signal, and regulation factors, characteristic of caulimovirus promoters, were present in each of these p ... | 2015 | 25947569 |
| transgenic alfalfa plants expressing the sweetpotato orange gene exhibit enhanced abiotic stress tolerance. | alfalfa (medicago sativa l.), a perennial forage crop with high nutritional content, is widely distributed in various environments worldwide. we recently demonstrated that the sweetpotato orange gene (ibor) is involved in increasing carotenoid accumulation and enhancing resistance to multiple abiotic stresses. in this study, in an effort to improve the nutritional quality and environmental stress tolerance of alfalfa, we transferred the ibor gene into alfalfa (cv. xinjiang daye) under the contro ... | 2015 | 25946429 |
| cyclic di-gmp acts as a cell cycle oscillator to drive chromosome replication. | fundamental to all living organisms is the capacity to coordinate cell division and cell differentiation to generate appropriate numbers of specialized cells. whereas eukaryotes use cyclins and cyclin-dependent kinases to balance division with cell fate decisions, equivalent regulatory systems have not been described in bacteria. moreover, the mechanisms used by bacteria to tune division in line with developmental programs are poorly understood. here we show that caulobacter crescentus, a bacter ... | 2015 | 25945741 |
| the all-alpha domains of coupling proteins from the agrobacterium tumefaciens virb/vird4 and enterococcus faecalis pcf10-encoded type iv secretion systems confer specificity to binding of cognate dna substrates. | bacterial type iv coupling proteins (t4cps) bind and mediate the delivery of dna substrates through associated type iv secretion systems (t4sss). t4cps consist of a transmembrane domain, a conserved nucleotide-binding domain (nbd), and a sequence-variable helical bundle called the all-alpha domain (aad). in the t4cp structural prototype, plasmid r388-encoded trwb, the nbd assembles as a homohexamer resembling reca and dna ring helicases, and the aad, which sits at the channel entrance of the hom ... | 2015 | 25939830 |
| enhancing t-dna transfer efficiency in barley (hordeum vulgare l.) cells using extracellular cellulose and lectin. | a major limitation of transforming barley tissues by agrobacterium tumefaciens is the low frequency of t-dna transfer due to recalcitrance of barley as a host. the effect of extracellular cellulose and lectin on agrobacterium transformation efficiency was investigated in this study. barley callus cultures were transformed with the agl1 strain containing the vector pbi121 in the presence of 10 mg ml(-1) cellulose or 0.001, 0.05 and 0.1 mg ml(-1) lectin. addition of cellulose significantly (p ≤ 0. ... | 2015 | 25935223 |
| genetic transformation of extremophilic fungi acidea extrema and acidothrix acidophila. | intact, growing cells of strongly acidophilic fungi acidea extrema and acidothrix acidophila have been successfully transformed by introduction of heterologous dna fragment (composed of the glyceraldehyde-phosphate-dehydrogenase gene promoter from emericella nidulans, a metallothionein-coding gene asmt1 from amanita strobiliformis and glyceraldehyde-phosphate-dehydrogenase gene terminator from colletotrichum gloeosporioides) with the length of 1690 bp. the transformation procedure was based on t ... | 2015 | 25934267 |
| overexpression of 3-deoxy-7-phosphoheptulonate synthase gene from gossypium hirsutum enhances arabidopsis resistance to verticillium wilt. | expression of dhs1 in cotton is induced upon infection by verticillium dahliae , and overexpression of ghdhs1 endows transgenic arabidopsis plants excellent verticillium resistance. verticillium wilt is caused by a soil-borne fungus verticillium dahliae. resistance in most cotton cultivars is either scarce or unavailable, making verticillium wilt a major obstacle in cotton production. here, we identified a 3-deoxy-7-phosphoheptulonate synthase (dhs, ec 4.1.2.15) gene from gossypium hirsutum, nam ... | 2015 | 25929795 |
| sonication, vacuum infiltration and thiol compounds enhance the agrobacterium-mediated transformation frequency of withania somnifera (l.) dunal. | in the present study, we have established a stable transformation protocol via agrobacterium tumafacines for the pharmaceutically important withania somnifera. six day-old nodal explants were used for 3 day co-cultivation with agrobacterium tumefaciens strain lba4404 harbouring the vector pcamiba2301. among the different injury treatments, sonication, vacuum infiltration and their combination treatments tested, a vacuum infiltration for 10 min followed by sonication for 10 sec with a. tumefacien ... | 2015 | 25927703 |
| proteomics and genetics for identification of a bacterial antimonite oxidase in agrobacterium tumefaciens. | antimony (sb) and its compounds are listed by the united states environmental protection agency (usepa, 1979) and the european union (cec, 1976) as a priority pollutant. microbial redox transformations are presumed to be an important part of antimony cycling in nature; however, regulation of these processes and the enzymology involved are unknown. in this study, comparative proteomics and reverse transcriptase-pcr analysis of sb(iii)-oxidizing bacterium agrobacterium tumefaciens gw4 revealed an ... | 2015 | 25909855 |
| identification of the genes involved in the fruiting body production and cordycepin formation of cordyceps militaris fungus. | a mutant library of cordyceps militaris was constructed by improved agrobacterium tumefaciens-mediated transformation and screened for degradation features. six mutants with altered characters in in vitro and in vivo fruiting body production, and cordycepin formation were found to contain a single copy t-dna. t-dna flanking sequences of these mutants were identified by thermal asymmetric interlaced-pcr approach. atp-dependent helicase, cytochrome oxidase subunit i and ubiquitin-like activating e ... | 2015 | 25892913 |
| current technologies and related issues for mushroom transformation. | mushroom transformation requires a series of experimental steps, including generation of host strains with a desirable selective marker, design of vector dna, removal of host cell wall, introduction of foreign dna across the cell membrane, and integration into host genomic dna or maintenance of an autonomous vector dna inside the host cell. this review introduces limitations and obstacles related to transformation technologies along with possible solutions. current methods for cell wall removal ... | 2015 | 25892908 |
| overexpression of a gmcnx1 gene enhanced activity of nitrate reductase and aldehyde oxidase, and boosted mosaic virus resistance in soybean. | molybdenum cofactor (moco) is required for the activities of moco-dependant enzymes. cofactor for nitrate reductase and xanthine dehydrogenase (cnx1) is known to be involved in the biosynthesis of moco in plants. in this work, a soybean (glycine max l.) cnx1 gene (gmcnx1) was transferred into soybean using agrobacterium tumefaciens-mediated transformation method. twenty seven positive transgenic soybean plants were identified by coating leaves with phosphinothricin, bar protein quick dip stick a ... | 2015 | 25886067 |