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variable adhesion of listeria monocytogenes isolates from food-processing facilities and clinical cases to inert surfaces.one hundred one strains of listeria monocytogenes isolated from seafood and cheese industry samples and from patients with listeriosis were assessed using a microtiter plate method for adhesion to polystyrene and stainless steel surfaces. the adhesion rate for these strains ranged from 3.10 to 35.29% with an inoculum of 8 x 10(8) cells per well. a strong correlation was found between adhesion to polystyrene and stainless steel microtiter plates, indicating that the intrinsic ability of l. monocy ...200717685327
inactivation of listeria monocytogenes on frankfurters by monocaprylin alone or in combination with acetic acid.the antilisterial activity of monocaprylin (mc) and its combination with acetic acid (aa) on frankfurters was investigated. each frankfurter was surface inoculated with a three-strain mixture of listeria monocytogenes to obtain an inoculation level of 4.0 log cfu per frankfurter, and then dipped for 35 s in sterile deionized water (45 or 50 degrees c) containing 1% ethanol (control), 50 mm mc plus 1% ethanol, 1% aa plus 1% ethanol, or 50 mm mc plus 1% aa plus 1% ethanol. samples were vacuum pack ...200717685330
could modifications of processing parameters enhance the growth and selection of lactic acid bacteria in cold-smoked salmon to improve preservation by natural means?several smoking conditions were examined with the objective of enhancing the numbers of lactic acid bacteria (lab) by natural means in vacuum-packaged cold-smoked salmon during 21 days of storage at 5 degrees c. three combinations of salting, drying, and smoking were used: (i) dry salting x time of salting (2 or 6 h); (ii) wet salting (6 h) x dry salting (6 h) x with or without sugar; and (iii) wet salting (6 h) x dry salting (6 h) x different times of smoking (2 or 6 h of drying and 2 or 6 h of ...200717685332
real-time pcr detection of 16s rrna genes speeds most-probable-number enumeration of foodborne listeria monocytogenes.quantifying foodborne pathogens at concentrations of 0.1 to 1,000 cfu/g of food generally involves most-probable-number (mpn) enumeration, which takes at least 4 days. a real-time pcr assay (rti-pcr) was developed to accelerate mpn enumeration of foodborne listeria monocytogenes. foods were spiked from 70 to 110 cfu/g, and triplicate subportions from 0.0001 to 1 g were selectively enriched for 48 h at 30 degrees c. for standard mpn enumeration, the enrichments were subcultured on oxford agar (48 ...200717685338
a novel multiplex pcr assay for rapid and simultaneous detection of five pathogenic bacteria: escherichia coli o157:h7, salmonella, staphylococcus aureus, listeria monocytogenes, and vibrio parahaemolyticus.rapid and sensitive detection techniques for foodborne pathogens are important to the food industry. however, traditional detection methods rely on bacterial culture in combination with biochemical tests, a process that typically takes 4 to 7 days to complete. thus, this study was conducted to address the issue of time lag inherent in traditional methods by developing a novel pcr assay for each of five foodborne pathogenic bacteria. this new system consists of a simultaneous screening method usi ...200717685339
microbiological characterization of randomly selected portuguese raw milk cheeses with reference to food safety.seventy raw milk cheeses made in different regions of portugal, both hard and soft varieties, made with cow's, ewe's, or goat's milk or combinations of these, were sampled within their quoted shelf lives for microbiological safety. on the basis of the presence or numbers of escherichia coli, e. coli o157, staphylococcus aureus, salmonella, and listeria monocytogenes, cheeses were categorized as satisfactory, acceptable, unsatisfactory, or unacceptable and potentially hazardous. twenty-two of the ...200717685347
nad kinases use substrate-assisted catalysis for specific recognition of nad.here we describe the crystal structures of the nad kinase (lmnadk1) from listeria monocytogenes in complex with its substrate nad, its product nadp, or two synthesized nad mimics. we identified one of the nad mimics, di-adenosine diphosphate, as a new substrate for lmnadk1, whereas we showed that the closely related compound di-5'-thioadenosine is a novel non-natural inhibitor for this enzyme. these structures suggest a mechanism involving substrate-assisted catalysis. indeed, sequence/structure ...200717686780
sequencing and expression analysis of sakacin genes in lactobacillus curvatus strains.in this study, we focused our investigation on two strains of lactobacillus curvatus, l442 and lth1174, which are able to produce bacteriocins. l. curvatus lth1174 is widely studied for its capability to produce curvacin a, while l. curvatus l442 was isolated from traditional greek fermented sausages and was shown to possess a strong inhibitory activity toward listeria monocytogenes. by polymerase chain reaction, we were able to target in both strains the genes for the production of sakacin p an ...200717690878
explaining unexplained diarrhea and associating risks and infections.gastrointestinal illnesses are common afflictions. however, knowledge of their etiology is often lacking. moreover, most cases of infections with reportable enteric pathogens (campylobacter jejuni, escherichia coli o157:h7, salmonella, shigella, yersinia, cryptosporidia and giardia) have sporadic modes of acquisition, yet control measures are often biased towards mitigation of risks discerned by outbreak analysis. to determine the etiology of unexplained diarrhea it is important to study populat ...200717692144
multiplex pcr for simultaneous detection of bacteria of the genus listeria, listeria monocytogenes, and major serotypes and epidemic clones of l. monocytogenes.a multiplex pcr assay which combines detection of bacteria of the genus listeria, listeria monocytogenes serotypes 1/2a and 4b, and epidemic clones i, ii, and iii of l. monocytogenes was developed. the assay provides a rapid, reliable, and inexpensive method for screening and subgrouping this important food-borne pathogen.200717693562
carnobacterium: positive and negative effects in the environment and in foods.the genus carnobacterium contains nine species, but only c. divergens and c. maltaromaticum are frequently isolated from natural environments and foods. they are tolerant to freezing/thawing and high pressure and able to grow at low temperatures, anaerobically and with increased co(2) concentrations. they metabolize arginine and various carbohydrates, including chitin, and this may improve their survival in the environment. carnobacterium divergens and c. maltaromaticum have been extensively stu ...200717696886
regional ifngamma expression is insufficient for efficacious control of food-borne bacterial pathogens at the gut epithelial barrier.ifngamma is critical for host defence against various food-borne pathogens including salmonella enterica and listeria monocytogenes, the causative agents of salmonellosis and listeriosis, respectively. we investigated the impact of regional ifngamma expression at the intestinal epithelial barrier on host invasion by salmonellae and listeriae following oral challenge. transgenic mice (ifngamma-gut), generated on an ifngamma knock-out (ko) background, selectively expressed ifngamma in the gut driv ...200717698562
memory cd8+ t cells mediate antibacterial immunity via ccl3 activation of tnf/roi+ phagocytes.cytolysis, interferon gamma and tumor necrosis factor (tnf) alpha secretion are major effector mechanisms of memory cd8+ t cells that are believed to be required for immunological protection in vivo. by using mutants of the intracellular bacterium listeria monocytogenes, we found that none of these effector activities is sufficient to protect against secondary infection with wild-type (wt) bacteria. we demonstrated that ccl3 derived from reactivated memory cd8+ t cells is required for efficient ...200717698589
a synthetic polypeptide based on human e-cadherin inhibits invasion of human intestinal and liver cell lines by listeria monocytogenes.internalin a is a surface protein of the facultative intracellular pathogen listeria monocytogenes that interacts with the human host cell protein e-cadherin to facilitate invasion of epithelial cells. a single amino acid substitution at position 16 in mouse e-cadherin prevents this interaction. synthetic polypeptides of 30 aa encompassing position 16 of human and mouse e-cadherin were tested for their ability to inhibit in vitro invasion of caco-2, hepg2 and tib73 cell lines by l. monocytogenes ...200717644706
clinical features of liver involvement in adult patients with listeriosis. review of the literature.clinical features of liver involvement due to listeria monocytogenes infection in adults are rarely reported in literature. this is surprising, regarding the current opinion that the portal system is extensively involved in the first stages of pathogenesis in invasive l. monocytogenes disease. a literature search in the pubmed and embase database revealed 34 cases with clinical features of hepatic involvement due to listeriosis. we systematically analyzed all case reports with respect to clinica ...200717646920
bacteriocin activity of enterococci from rabbits.the antimicrobial spectrum of bacteriocin-producing enterococcal isolates from rabbits faeces, the biochemical characterization of bacteriocins, and their molecular mass and the presence of structural genes for bacteriocin production were investigated. among enterococci selected from rabbit faeces, six strains of enterococcus faecium (ef2019, ef1819, ef2119, ef1839, ef529, ef24/10) showed inhibitory activity against the indicators e. avium ea5, listeria innocua lmg13568 and listeria monocytogene ...200717216320
flagellar motility is critical for listeria monocytogenes biofilm formation.the food-borne pathogen listeria monocytogenes attaches to environmental surfaces and forms biofilms that can be a source of food contamination, yet little is known about the molecular mechanisms of its biofilm development. we observed that nonmotile mutants were defective in biofilm formation. to investigate how flagella might function during biofilm formation, we compared the wild type with flagellum-minus and paralyzed-flagellum mutants. both nonmotile mutants were defective in biofilm develo ...200717416647
identification of the sigmab regulon of bacillus cereus and conservation of sigmab-regulated genes in low-gc-content gram-positive bacteria.the alternative sigma factor sigma(b) has an important role in the acquisition of stress resistance in many gram-positive bacteria, including the food-borne pathogen bacillus cereus. here, we describe the identification of the set of sigma(b)-regulated genes in b. cereus by dna microarray analysis of the transcriptome upon a mild heat shock. twenty-four genes could be identified as being sigma(b) dependent as witnessed by (i) significantly lower expression levels of these genes in mutants with a ...200717416654
entry of the bacterial pathogen listeria monocytogenes into mammalian cells.the bacterial pathogen listeria monocytogenes causes food-borne illnesses leading to meningitis or abortion. listeria provokes its internalization ('entry') into mammalian cells that are normally non-phagocytic, such as intestinal epithelial cells and hepatocytes. entry provides access to a nutrient-rich cytosol and allows translocation across anatomical barriers. here i discuss the two major internalization pathways used by listeria. these pathways are initiated by binding of the bacterial surf ...200717419717
the multiple mechanisms of ca2+ signalling by listeriolysin o, the cholesterol-dependent cytolysin of listeria monocytogenes.cholesterol-dependent cytolysins (cdcs) represent a large family of conserved pore-forming toxins produced by several gram-positive bacteria such as listeria monocytogenes, streptococcus pyrogenes and bacillus anthracis. these toxins trigger a broad range of cellular responses that greatly influence pathogenesis. using mast cells, we demonstrate that listeriolysin o (llo), a prototype of cdcs produced by l. monocytogenes, triggers cellular responses such as degranulation and cytokine synthesis i ...200717419718
jinx, an mcmv susceptibility phenotype caused by disruption of unc13d: a mouse model of type 3 familial hemophagocytic lymphohistiocytosis.mouse cytomegalovirus (mcmv) susceptibility often results from defects of natural killer (nk) cell function. here we describe jinx, an n-ethyl-n-nitrosourea-induced mcmv susceptibility mutation that permits unchecked proliferation of the virus, causing death. in jinx homozygotes, activated nk cells and cytotoxic t lymphocytes (ctls) fail to degranulate, although they retain the ability to produce cytokines, and cytokine levels are markedly elevated in the blood of infected mutant mice. jinx was ...200717420270
characterization of the osmoprotectant transporter opuc from pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function.the plant pathogen pseudomonas syringae may cope with osmotic stress on plants, in part, by importing osmoprotective compounds. in this study, we found that p. syringae pv. tomato strain dc3000 was distinct from most bacterial species in deriving greater osmoprotection from exogenous choline than from glycine betaine. this superior osmoprotection was correlated with a higher capacity for uptake of choline than for uptake of glycine betaine. of four putative osmoregulatory abc transporters in dc3 ...200717660277
recombination and positive selection contribute to evolution of listeria monocytogenes inla.the surface molecule inla interacts with e-cadherin to promote invasion of listeria monocytogenes into selected host cells. dna sequencing of inla for 40 l. monocytogenes isolates revealed 107 synonymous and 45 nonsynonymous substitutions. a frameshift mutation in a homopolymeric tract encoding part of the inla signal peptide was identified in three lineage ii isolates, which also showed reduced ability to invade human intestinal epithelial cells. phylogenies showed clear separation of inla sequ ...200717660431
modelling transfer of listeria monocytogenes during slicing of 'gravad' salmon.transfer of a rifampicin-resistant mutant of listeria monocytogenes from an inoculated slicing blade to slices of 'gravad' salmon (salmo salar), and from inoculated salmon fillet to the slicing machine and subsequently to slices of uninoculated fillet was studied. the effect of slicing temperature (0 degrees c, 10 degrees c and room temperature), inoculum level (approx. 3, 5 and 8 log cfu/blade), and attachment time of inoculum to blade (10 min and 2.5 h) were investigated and predictive models ...200717651853
antioxidant and antibacterial activities of aqueous extract of seabuckthorn (hippophae rhamnoides) seeds.seabuckthorn (hippophae rhamnoides) seeds aqueous extract was screened for antioxidant and antibacterial activities. the antioxidant activities (reducing power, dpph and liposome model system) showed a good antioxidant activity. the extract was also found to possess antibacterial activity with a mic values with respect to listeria monocytogenes and yersinia enterocolitica found to be 750 and 1000 ppm, respectively. the antioxidant and antimicrobial effects of the extract implicate its potential ...200717651916
cell-free extract systems and the cytoskeleton: preparation of biochemical experiments for transmission electron microscopy.cell-free systems can be used to reconstitute complex actin or microtubule-based phenomena. for example, a number of extracts support actin-dependent propulsion of listeria monocytogenes, whereas xenopus laevis extracts support formation of a microtubule-based meiotic spindle. working in vitro opens these complex processes to biochemical dissection. here, we describe methods to view these in vitro preparations by thin-section electron microscopy.200717656752
changes in listeria monocytogenes membrane fluidity in response to temperature stress.listeria monocytogenes is a food-borne pathogen that has been implicated in many outbreaks associated with ready-to-eat products. listeria adjusts to various stresses by adjusting its membrane fluidity, increasing the uptake of osmoprotectants and cryoprotectants, and activating the sigma(b) stress factor. the present work examines the regulation of membrane fluidity through direct measurement based on fluorescent anisotropy. the membrane fluidities of l. monocytogenes scott a, nr30, wt10403s, a ...200717704268
comparative transcriptome analysis of listeria monocytogenes strains of the two major lineages reveals differences in virulence, cell wall, and stress response.listeria monocytogenes is a food-borne, opportunistic, bacterial pathogen causing a wide spectrum of diseases, including meningitis, septicemia, abortion, and gastroenteritis, in humans and animals. among the 13 l. monocytogenes serovars described, human listeriosis is mostly associated with strains of serovars 4b, 1/2b, and 1/2a. within the species l. monocytogenes, three phylogenetic lineages are described. serovar 1/2a belongs to phylogenetic lineage i, while serovars 4b and 1/2b group in phy ...200717704270
effect of short-term stainless steel welding fume inhalation exposure on lung inflammation, injury, and defense responses in rats.many welders have experienced bronchitis, metal fume fever, lung function changes, and an increase in the incidence of lung infection. questions remain regarding the possible mechanisms associated with the potential pulmonary effects of welding fume exposure. the objective was to assess the early effects of stainless steel (ss) welding fume inhalation on lung injury, inflammation, and defense responses. male sprague-dawley rats were exposed to gas metal arc-ss welding fume at a concentration of ...200717706736
examination of host immune resistance against listeria monocytogenes infection in cyclophosphamide-treated mice after dietary lipid administration.despite the beneficial effects in the resolution of inflammatory disorders due to their immunosuppressive properties, n-3 polyunsaturated fatty acids are associated with a reduction of immune resistance to some microorganisms. here, we examine the influence of different dietary lipids on host immune resistance against listeria monocytogenes in mice treated with cyclophosphamide (cpa).200717707555
listeria monocytogenes following orthotopic liver transplantation: central nervous system involvement and review of the literature.listeria monocytogene is a well-recognized cause of bacteremia in immunocompromised individuals, including solid organ transplant recipients, but has been rarely reported following orthotopic liver transplantation. we describe a case of listeria meningitis that occurred within a week after liver transplantation. the patient developed a severe headache that mimicked tacrolimus encephalopathy, and was subsequently diagnosed with listeria meningitis by cerebrospinal fluid culture. the infection was ...200717708617
classification of a moderately oxygen-tolerant isolate from baby faeces as bifidobacterium thermophilum.bifidobacteria are found at varying prevalence in human microbiota and seem to play an important role in the human gastrointestinal tract (git). bifidobacteria are highly adapted to the human git which is reflected in the genome sequence of a bifidobacterim longum isolate. the competitiveness against other bacteria is not fully understood yet but may be related to the production of antimicrobial compounds such as bacteriocins. in a previous study, 34 bifidobacterium isolates have been isolated f ...200717711586
occurrence of salmonella and listeria spp. on retail poultry products in south italy and comparison of conventional and rapid methods for their detection.salmonella and listeria spp. are frequently detected in poultry meats. conventional isolation and identification methods to detect these microrganisms in food are laborious and time-consuming. in the present study the occurrence of salmonellae and listeriae on 362 samples of retail poultry in caserta, south italy was evaluated and standard microbiological and rapid methods were compared. furthermore, the samples were collected and analyzed twice a week, on monday and friday to establish their po ...200717713141
regulation of iga production by naturally occurring tnf/inos-producing dendritic cells.immunoglobulin-a has an irreplaceable role in the mucosal defence against infectious microbes. in human and mouse, iga-producing plasma cells comprise approximately 20% of total plasma cells of peripheral lymphoid tissues, whereas more than 80% of plasma cells produce iga in mucosa-associated lymphoid tissues (malt). one of the most biologically important and long-standing questions in immunology is why this 'biased' iga synthesis takes place in the malt but not other lymphoid organs. here we sh ...200717713535
modelling the behaviour of listeria monocytogenes in ground pork as a function of ph, water activity, nature and concentration of organic acid salts.to study and model the effect of sodium acetate, sodium lactate, potassium sorbate and combination of acid salts on the behaviour of listeria monocytogenes in ground pork.200717714386
probiotic properties of lactococcus lactis ssp. lactis hv219, isolated from human vaginal secretions.to determine the resistance of lactococcus lactis ssp. lactis hv219 to acids, bile, antibiotics, inflammatory drugs and spermicides, compare adsorption of the strain to bacteria and caco-2 cells under stress, and evaluate the antimicrobial activity of bacteriocin hv219.200717714396
thermodynamically reengineering the listerial invasion complex inla/e-cadherin.biological processes essentially all depend on the specific recognition between macromolecules and their interaction partners. although many such interactions have been characterized both structurally and biophysically, the thermodynamic effects of small atomic changes remain poorly understood. based on the crystal structure of the bacterial invasion protein internalin (inla) of listeria monocytogenes in complex with its human receptor e-cadherin (hec1), we analyzed the interface to identify sin ...200717715295
potential of surface-enhanced raman spectroscopy for the rapid identification of escherichia coli and listeria monocytogenes cultures on silver colloidal nanoparticles.surface-enhanced raman (sers) spectra of various batches of bacteria adsorbed on silver colloidal nanoparticles were collected to explore the potential of the sers technique for rapid and routine identification of e. coli and l. monocytogenes cultures. relative standard deviation (rsd) of sers spectra from silver colloidal suspensions and ratios of sers peaks from small molecules (k(3)po(4)) were used to evaluate the reproducibility, stability, and binding effectiveness of citrate-reduced silver ...200717716400
rapid detection of listeria monocytogenes by nanoparticle-based immunomagnetic separation and real-time pcr.the objective of this study was to develop a method combining nanoparticle-based immunomagnetic separation (ims) with real-time pcr for a rapid and quantitative detection of listeria monocytogenes. carboxyl modified magnetic nanoparticles were covalently bound with rabbit anti-l. monocytogenes via the amine groups. several factors, such as the amount of immunomagnetic nanoparticles (imnps), reaction and collection times, and washing step, were optimized, and the nanoparticle-based ims in combina ...200717716768
low genetic diversity and epidemiological significance of listeria monocytogenes isolated from wild animals in the far east of russia.the causative agent of listeriosis, a serious disease of humans and animals, listeria monocytogenes is a ubiquitous bacterium that inhabits both anthropogenic and pristine environments. we report l. monocytogenes isolation from wild animals, humans, food and the environment of a far eastern region of russia. in total, 654 samples of internal organs of small rodents belonging to the muridae and cricetidae families, and 986 samples of the liver and muscles of mollusks and fish were examined to obt ...200717716956
in vitro inhibition activity of nisin a, nisin z, pediocin pa-1 and antibiotics against common intestinal bacteria.to evaluate the sensitivity of 21 common intestinal bacteria to six antibiotics and three broad-spectrum bacteriocins (nisins z and a and pediocin pa-1).200717718835
listeria as a vaccine vector.the immunostimulatory characteristics and intracellular niche of listeria monocytogenes make it uniquely suitable for use as a live bacterial vaccine vector. preclinical results supporting this idea, and current strategies to induce beneficial cell-mediated immunity to both infectious diseases and cancer with this vector, are discussed in this review.200717719258
innate and adaptive immune responses to listeria monocytogenes: a short overview.the gram-positive facultative intracellular bacterium listeria monocytogenes is a model pathogen for elucidating important mechanisms of the immune response. infection of mice with a sub-lethal dose of bacteria generates highly reproducible innate and adaptive immune responses, resulting in clearance of the bacteria and resistance to subsequent l. monocytogenes infection. both the innate and adaptive immune systems are crucial to the recognition and elimination of this pathogen from the host.200717719259
molecular mechanisms exploited by listeria monocytogenes during host cell invasion.the facultative intracellular bacterial pathogen listeria monocytogenes has evolved multiple strategies to invade a large panel of mammalian cells. host cell invasion is critical for several stages of listeriosis pathology such as the initial crossing of the host intestinal barrier and the successive colonization of diverse target organs including the placenta. in this review, we address the main molecular mechanisms known to be used by l. monocytogenes during invasion of nonphagocytic cells and ...200717761447
the prfa virulence regulon.the prfa protein, a member of the crp/cap-fnr family of bacterial transcription factors, controls the expression of key virulence determinants of the facultative intracellular pathogen listeria monocytogenes. each of the steps of the listerial intracellular infection cycle-host cell invasion, phagosomal escape, cytosolic replication, and direct cell-to-cell spread-is mediated by products of the prfa regulon. only 10 of the 2853 genes of the l. monocytogenes egde genome have been confirmed as bon ...200717764998
internalins: a complex family of leucine-rich repeat-containing proteins in listeria monocytogenes.the listeria monocytogenes genome includes a large family of proteins harbouring leucine-rich repeats known as internalins (inl). the generation of novel mutants and comparative analysis of inl variability among listeria and other bacterial genomes suggest that beyond the extensively-studied invasins, inla and inlb, additional internalins also play important functions in the infectious process.200717764999
enterococcus faecalis constitutes an unusual bacterial model in lysozyme resistance.lysozyme is an important and widespread compound of the host constitutive defense system, and it is assumed that enterococcus faecalis is one of the few bacteria that are almost completely lysozyme resistant. on the basis of the sequence analysis of the whole genome of e. faecalis v583 strain, we identified two genes that are potentially involved in lysozyme resistance, ef_0783 and ef_1843. protein products of these two genes share significant homology with staphylococcus aureus peptidoglycan o- ...200717785473
microbiological baseline study of swine carcasses at swedish slaughterhouses.this 13-month survey was conducted to estimate the prevalence and counts of foodborne pathogenic bacteria and indicator bacteria on swine carcasses in sweden. a total of 541 swine carcasses were sampled by swabbing prechill at the 10 largest slaughterhouses in sweden. pathogenic yersinia enterocolitica was detected by pcr in 16% of the samples. the probability of finding y. enterocolitica increased with increasing counts of escherichia coli. no samples were positive for salmonella. the prevalenc ...200717803133
enhancing antimicrobial activity of lysozyme against listeria monocytogenes using immunonanoparticles.the antimicrobial efficacy of lysozyme may be reduced by undesirable interactions with food components and nontarget bacteria. immunonanoparticles, i.e., nanoparticles functionalized with pathogen-specific antibodies, may serve as an antimicrobial carrier for improving the stability and activity of antimicrobials in foods. the objective of this research was to study the antimicrobial activity of lysozyme-carrying immunonanoparticles against listeria monocytogenes. polystyrene nanoparticles with ...200717803140
combined use of ultrasound and natural antimicrobials to inactivate listeria monocytogenes in orange juice.the presence of listeria monocytogenes could seriously affect the safety of nonpasteurized fruit juices. high-intensity ultrasound combined with mild heat treatment and natural antimicrobials may be an alternative technology for fruit juice preservation. the response of l. monocytogenes in orange juice to combined treatments involving moderate temperature (45 degrees c), high-intensity ultrasound (600 w, 20 khz, 95.2 microm wave amplitude), and the addition of different levels of vanillin (0, 1, ...200717803141
aerosol studies with listeria innocua and listeria monocytogenes.aerosol studies of listeria monocytogenes in food processing plants have been limited by lack of a suitable surrogate microorganism. the objective of this study was to investigate the potential of using green fluorescent protein-labeled strains of listeria innocua as a surrogate for l. monocytogenes for aerosol studies. these studies were conducted in a laboratory bioaerosol chamber and a pilot food-processing facility. four strains of l. innocua and five strains of l. monocytogenes were used. i ...200717803142
an 8-year surveillance of the diversity and persistence of listeria monocytogenes in a chilled food processing plant analyzed by amplified fragment length polymorphism.contamination routes of listeria monocytogenes were examined in a chilled food processing plant that produced ready-to-eat and ready-to-reheat meals during an 8-year period by amplified fragment length polymorphism (aflp) analysis. a total of 319 l. monocytogenes isolates were recovered from raw materials (n = 18), the environment (n = 77), equipment (n = 193), and products (n = 31), and 18 different aflp types were identified, five of which were repeatedly found to be persistent types. the thre ...200717803143
comparison of multiplex pcr with conventional biochemical methods for the identification of listeria spp. isolates from food and clinical samples in queensland, australia.listeria monocytogenes is an important foodborne pathogen with high mortality. l. monocytogenes and five other listeria species can frequently be found in the same sample. to identify listeria isolates found in foods to the species level, two multiplex pcrs were designed. the pcr and conventional biochemical methods were compared for the identification of 456 listeria isolates collected from routine food quality monitoring schemes between june 2004 and february 2006 and for 62 l. monocytogenes i ...200717803144
shelf life establishment of a sliced, cooked, cured meat product based on quality and safety determinants.in the present study, the distribution of the shelf life of cooked, cured meat products based on lactic acid bacteria growth and the distribution of the time to cause health risks based on listeria monocytogenes growth were studied. growth models, developed and validated on cooked meat products, were used to predict the growth of microorganisms. temperature data were obtained from retail and home refrigerators. distribution predictions were conducted by two approaches (time-temperature profiles ...200717803145
occurrence of listeria monocytogenes in sandwiches available to hospital patients in wales, united kingdom.a survey for the presence of listeria monocytogenes in hospital sandwiches was carried out in wales, united kingdom, between october 2005 and march 2006. the main aim of the survey was to establish the baseline rate of l. monocytogenes in hospital sandwiches after an outbreak of listeriosis among hospital patients in 2004 was epidemiologically linked to the consumption of contaminated sandwiches. the overall positive rate found in hospital sandwiches was 2.84% for enriched culture and 0.21% for ...200717803157
development of multilocus single strand conformation polymorphism (mlsscp) analysis of virulence genes of listeria monocytogenes and comparison with existing dna typing methods.development of rapid and simple typing methods is required for analyzing the distribution and contamination routes of food-borne pathogens. we established a simple typing method for listeria monocytogenes using mlsscp (multilocus single strand conformation polymorphism) analysis. four virulence genes, hlya, iap, acta and inlb were amplified by pcr, digested with endonucleases and applied to gels for sscp. as banding patterns have been shown to reflect even a single nucleotide difference, this me ...200717822795
[evaluation of the effect of lactobacillus rhamnosus probiotic culture added to yogurt over staphylococcus aureus, escherichia coli o157:h7, listeria monocytogenes and salmonella enteritidis populations].the effect of different types of probiotics present in yogurt over known populations of staphylococcus aureus, escherichia coli o157:h7, listeria monocytogenes and salmonella enteritidis was evaluated. the three types of yogurt used were: without added probiotics, with added probiotics (lactobacillus casei crl_431 and l. acidophilus crl_730 chr hansen) and another one with the same probiotics mentioned above and lactobacillus rhamnosus (lr-35) culture. about 10(9) cfu/ ml of each potentially pat ...200717824199
irf3 polymorphism alters induction of interferon beta in response to listeria monocytogenes infection.genetic makeup of the host plays a significant role in the course and outcome of infection. inbred strains of mice display a wide range of sensitivities to listeria monocytogenes infection and thus serve as a good model for analysis of the effect of genetic polymorphism. the outcome of l. monocytogenes infection in mice is influenced by the ability of this bacterium to induce expression of interferon beta mrna, encoded in mouse by the ifnb1 (interferon beta 1, fibroblast) gene. mouse strains tha ...200717845078
antimicrobial effect of halocidin-derived peptide in a mouse model of listeria infection.halocidin is an antimicrobial peptide found in the tunicate. a series of experiments were previously conducted in an attempt to develop a novel antibiotic derived from halocidin, as the peptide was determined to evidence profound antimicrobial activity against a variety of antibiotic-resistant microbes, with significantly less toxicity to human blood cells. in this study, we assessed the validity of one of the halocidin congeners, called khal, as a new antibiotic for the treatment of systemic ba ...200717846130
the carboxyl-terminal sh3 domain of the mammalian adaptor crkii promotes internalization of listeria monocytogenes through activation of host phosphoinositide 3-kinase.the intracellular bacterial pathogen listeria monocytogenes causes food-borne illnesses leading to gastroenteritis, meningitis or abortion. listeria induces its internalization into some mammalian cells through binding of the bacterial surface protein inlb to its host receptor, the met receptor tyrosine kinase. inlb-induced activation of met stimulates host signal transduction pathways that culminate in cell surface changes driving pathogen engulfment. one mammalian protein with the potential to ...200717848169
detection of the pediocin gene peda in strains from human faeces by real-time pcr and characterization of pediococcus acidilactici uva1.bacteriocin-producing lactic acid bacteria are commonly used as natural protective cultures. among them, strains of the genus pediococcus are particularly interesting for their ability to produce pediocin, a broad spectrum antimicrobial peptide with a strong antagonistic activity against the food-borne pathogen listeria monocytogenes. furthermore, there is increasing interest in isolating new bacteriocin-producing strains of human intestinal origin that could be developed for probiotic effects a ...200717850651
live attenuated listeria monocytogenes expressing hiv gag: immunogenicity in rhesus monkeys.induction of strong cellular immunity will be important for aids vaccine candidates. natural infection with wild-type listeria monocytogenes (lm), an orally transmitted organism, is known to generate strong cellular immunity, thus raising the possibility that live attenuated lm could serve as a vaccine vector. we sought to examine the potential of live attenuated lm to induce cellular immune responses to hiv gag. rhesus macaques were immunized with lmdd-gag that expresses hiv gag and lacks two g ...200717854955
an accelerated vaccine schedule with a poly-antigenic hepatitis c virus mva-based candidate vaccine induces potent, long lasting and in vivo cross-reactive t cell responses.we designed and evaluated in hla-class i transgenic mouse models a hepatitis c virus (hcv) t cell-based mva vectored vaccine expressing three viral antigens known to be targets of potent cd8+- and cd4+-mediated responses. an accelerated (3 week-based) vaccination induced specific cd8+ t cells harboring two effector functions (cytolytic activity - both in vitro and in vivo- and production of ifngamma) as well as specific cd4+ t cells recognizing all three vaccine antigens. responses were long las ...200717875349
establishment of procedures provoking sub-lethal injury of listeria monocytogenes, campylobacter jejuni and escherichia coli o157 to serve method performance testing.in this study procedures provoking sub-lethal injury for three different pathogens are described which may be used in determination of accuracy and robustness of methods, comparison studies and or validation of rapid detection methods. three common food-borne pathogens were used, listeria monocytogenes, campylobacter jejuni and escherichia coli o157. the pathogens were exposed to heat stress, cold stress, freeze stress, acid stress, oxidative stress and "food" stress. sub-lethal injury was deter ...200717719670
biodiversity of the species listeria monocytogenes and the genus listeria.this review describes the listeria monocytogenes genome sequences available today and their comparison with that of listeria innocua and listeria welshimeri by highlighting their characteristic features and common traits. the diversity present among them is analysed with emphasis on putative virulence and host-pathogen interaction related functions. then large-scale studies comparing gene content of listeria and how these studies contributed to typing applications will be discussed. finally, evo ...200717719817
life of listeria monocytogenes in the host cells' cytosol.in the past decades impressive knowledge has been accumulated concerning the basic mechanisms of interactions between intracellular bacteria and their host cells. comparatively little is known on the metabolic requirements necessary for efficient replication of these bacteria within their specific host cell compartments. recent developments in functional genomics have led to more extensive studies of the metabolic aspects that may be crucial for understanding the pathogenesis of intracellular ba ...200717719818
human listeriosis and animal models.human listeriosis is a potentially fatal foodborne infection caused by listeria monocytogenes, an opportunistic psychrophile bacterium that is widespread in the environment. it has only recently emerged as a significant cause of human infection in industrialized countries, owing to appearance of a vulnerable population of immunocompromised individuals, and the concomitant development of large-scale agro-industrial plants and refrigerated food. here we review the main clinical features of human l ...200717720601
the epidemiology of human listeriosis.listeriosis is a serious invasive disease that primarily afflicts pregnant women, neonates and immunocompromised adults. the causative organism, listeria monocytogenes, is primarily transmitted to humans through contaminated foods. outbreaks of listeriosis have been reported in north america, europe and japan. soft cheeses made from raw milk and ready-to-eat meats are high risk foods for susceptible individuals. efforts by food processors and food regulatory agencies to aggressively control l. m ...200717720602
listeriolysin o: a phagosome-specific lysin.listeriolysin o (llo) is a pore-forming toxin of the cholesterol-dependent cytolysin family and a primary virulence factor of the gram-positive, facultative intracellular pathogen listeria monocytogenes. during the intracellular life cycle of l. monocytogenes, llo is largely responsible for mediating rupture of the phagosomal membrane, thereby allowing the bacterium access to the host cytosol, its replicative niche. in the host cytosol, llo activity is controlled at numerous levels to prevent pe ...200717720603
microarray-based characterization of the listeria monocytogenes cold regulon in log- and stationary-phase cells.whole-genome microarray experiments were performed to define the listeria monocytogenes cold growth regulon and to identify genes differentially expressed during growth at 4 and 37 degrees c. microarray analysis using a stringent cutoff (adjusted p < 0.001; >/=2.0-fold change) revealed 105 and 170 genes that showed higher transcript levels in logarithmic- and stationary-phase cells, respectively, at 4 degrees c than in cells grown at 37 degrees c. a total of 74 and 102 genes showed lower transcr ...200717720827
[study on molecular typing by pulsed-field gel electrophoresis of food listeria monocytogenes isolates from eight province (municipalities) of china].to analyse the molecular types of listeria (l.) monocytogenes, and to construct the standard china l. monocytogenes pulsed-field gel electrophoresis (pfge) subtyping database, using the international standardized l. monocytogenes-pfge protocol.200717877176
pathogen-specific cd8 t cell responses are directly inhibited by il-10.regulation of cd8 t cell expansion and contraction is essential for successful immune defense against intracellular pathogens. il-10 is a regulatory cytokine that can restrict t cell responses by inhibiting apc functions. il-10, however, can also have direct effects on t cells. although blockade or genetic deletion of il-10 enhances t cell-mediated resistance to infections, the extent to which il-10 limits in vivo apc function or t cell activation/proliferation remains unknown. herein, we demons ...200717878348
early bacterial dependent induction of inducible nitric oxide synthase (inos) in epithelial cells upon transfer of cd45rb(high) cd4(+) t cells in a model for experimental colitis.both the role of inducible nitric oxide synthase (inos) in the development of inflammatory bowel disease (ibd) as well as the molecular details governing its mucosal induction remain unclear.200717879278
development of flexible antimicrobial films using essential oils as active agents.the antimicrobial activity in the vapor-phase of laboratory-made flexible films of polypropylene (pp) and polyethylene/ethylene vinyl alcohol copolymer (pe/evoh) incorporating essential oil of cinnamon ( cinnamomum zeylanicum), oregano ( origanum vulgare), clove ( syzygium aromaticum), or cinnamon fortified with cinnamaldehyde was evaluated against a wide range of microorganisms: the gram-negative bacteria escherichia coli, yersinia enterocolitica, pseudomonas aeruginosa, and salmonella cholerae ...200717880148
construction and evaluation of multisite recombinatorial (gateway) cloning vectors for gram-positive bacteria.the gateway recombinatorial cloning system allows easy and rapid joining of dna fragments. here we report the construction and evaluation of three different gram-positive vectors that can be used with the multisite gateway cloning system to rapidly produce new gene arrangements in plasmid constructs for use in a variety of gram-positive bacteria.200717880697
invasive pathway of listeria ivanovii in human amnion-derived wish cells.among listeria genus, only two species, listeria ivanovii and listeria monocytogenes, are pathogenic. l. ivanovii is almost only associated with infections in animals, mainly sheep and cattle, and has rarely been associated with human infections, whereas l. monocytogenes causes severe illnesses in both humans and animals. to further investigate the pathogenetic features of l. ivanovii in humans, we undertook a study in which the intracellular behaviour of this pathogen was analysed in wish cells ...200717880764
[influence of different types of mineral fertilizers on reproduction of listeria monocytogenes in soil].influence of mineral fertilizers on the reproduction of listeria monocytogenes in soil ecosystems under different temperatures (4-6 degrees c and 20-22 degrees c) was studied. application of the fertilizers favored preservation and active reproduction of l. monocytogenes in brown podzolic soil and brown forest soil. duration of application of the mineral fertilizers positively correlated with preservation and reproduction of l. monocytogenes in different types of soil.200717882836
listeria monocytogenes meningitis: an uncommon opportunistic infection in hiv/aids.to report an interesting case of meningitis caused by listeria monocytogenes meningitis in an hiv seropositive individual.200717883181
prevalence and antimicrobial resistance of foodborne pathogens isolated from food products in china.this study was undertaken to investigate the prevalence of salmonella, listeria monocytogenes, staphylococcus aureus, vibrio parahaemolyticus, and escherichia coli o157:h7 in chinese food products. the prevalence of these pathogens was 3.46%, 5.79%, 7%, 0.24%, and 0%, respectively. raw meats were mainly contaminated with salmonella (39/365, 10.7%), l. monocytogenes (26/365, 7.1%), and s. aureus (40/365, 11%), while cooked food products were mainly contaminated with l. monocytogenes (45/384, 11.7 ...200717883311
a multiplex pcr for species- and virulence-specific determination of listeria monocytogenes.listeria monocytogenes internalin gene inlj has been described previously for differentiation of virulent from avirulent strains. however, a recent report indicated that there exist some unusual lineage iiib strains (e.g., serotype 7 strain r2-142) that possess no inlj gene but have the capacity to cause mouse mortality via intraperitoneal inoculation. therefore, a multiplex pcr incorporating inla, inlc and inlj gene primers was developed in this study for rapid speciation and virulence determin ...200717884210
in vitro study on bacteriocin production of enterococci associated with chickens.in recent years, the approach of using innovative strategies such as probiotics or bacteriocins for the prevention or treatment of bacterial infections has come into focus. the present study was undertaken to check in vitro ability of enterococci-isolated from the gastrointestinal tract of chickens-to produce a bacteriocin-like substance and to describe some further probiotic properties in five selected enterococcus faecium strains. all strains (n=17) were found to produce bacteriocin-like subst ...200717884622
screening of the enterocin genes and antimicrobial activity against pathogenic bacteria in enterococcus strains obtained from different origins.antimicrobial activities of 139 enterococcus isolates (48 e. faecium and 91 e. faecalis) obtained from canine feces, boiler meat samples, swine feces, wild waterfowl feces, and human feces were examined against respective bacteria, including streptococcus pyogenes, staphylococcus aureus, bacillus subtilis, listeria monocytogenes, salmonella enteritidis, and escherichia coli. bacteriocin (bac) production assay revealed that the antimicrobial activity against at least one of 6 indicator strains (b ...200718176018
effect of inhibitory extracts derived from liquid smoke combined with postprocess pasteurization for control of listeria monocytogenes on ready-to-eat meats.surface pasteurization was examined in combination with low-phenolic antimicrobial extracts derived from liquid smoke to inhibit and prevent the growth of listeria monocytogenes during the shelf life of ready-to-eat meats. in preliminary trials with retail frankfurters, one smoke derivative (2-min dip) produced a 0.3-log reduction of l. monocytogenes and a 1-min in-bag pasteurization (73.9 degrees c) produced a 2.9-log reduction, whereas a combination of the two treatments produced a 5.3-log red ...200718095426
antimicrobial activity of clove and cinnamon essential oils against listeria monocytogenes in pasteurized milk.the antimicrobial activity of essential oils (eos) of cinnamon bark, cinnamon leaf, and clove against listeria monocytogenes scott a were studied in semiskimmed milk incubated at 7 degrees c for 14 days and at 35 degrees c for 24 h. the mic was 500 ppm for cinnamon bark eo and 3,000 ppm for the cinnamon leaf and clove eos. these effective concentrations increased to 1,000 ppm for cinnamon bark eo, 3,500 ppm for clove eo, and 4,000 ppm for cinnamon leaf eo when the semiskimmed milk was incubated ...200718095427
pulsed-plasma gas-discharge inactivation of microbial pathogens in chilled poultry wash water.a pulsed-plasma gas-discharge (ppgd) system was developed for the novel decontamination of chilled poultry wash water. treatment of poultry wash water in the plasma generation chamber for up to 24 s at 4 degrees c reduced escherichia coli nctc 9001, campylobacter jejuni atcc 33560, campylobacter coli atcc 33559, listeria monocytogenes nctc 9863, salmonella enterica serovar enteritidis atcc 4931, and s. enterica serovar typhimurium atcc 14028 populations to non-detectable levels (< or = 8 log cfu ...200718095434
comparing uncertainty resulting from two-step and global regression procedures applied to microbial growth models.two different microbial modeling procedures were compared and validated against independent data for listeria monocytogenes growth. the most generally used method is two consecutive regressions: growth parameters are estimated from a primary regression of microbial counts, and a secondary regression relates the growth parameters to experimental conditions. a global regression is an alternative method in which the primary and secondary models are combined, giving a direct relationship between exp ...200718095435
different enrichment procedures for recovery of listeria monocytogenes from raw chicken samples can affect the results of detection (by chromogenic plating or real-time pcr) and lineage or strain identification.this study aimed to evaluate the effect of different enrichment procedures on the detection of listeria monocytogenes in food, by a comparison of subculture onto chromogenic agar with real-time pcr. two different culture media, the primary and secondary enrichment broths of the u.s. food safety and inspection service (fsis) method used for pcr detection of l. monocytogenes, were compared for the primary enrichment of retail ground chicken samples. l. monocytogenes was detected after the completi ...200718095442
bacterial contamination of sago starch in papua new guinea.sago starch is an important food in lowland papua new guinea. extraction of the starch from the palm and storage were performed by way of traditional methods that have been used for thousands of years. currently, very little is known about the microbiology of sago starch. sago samples were collected from areas of high starch utilization and analyzed for the presence of bacterial pathogens and indicator organisms. storage methods and duration were recorded at the time of collection, and ph and wa ...200718095446
cleaning and handling implements as potential reservoirs for bacterial contamination of some ready-to-eat foods in retail delicatessen environments.this study assessed the association of bacteria with cleaning tools, such as floor mops (n = 25) and cleaning cloths (n = 39), and handling devices, such as disposable plastic gloves (n = 20), used during filled baguette and assorted salad preparation in four selected retail delicatessens in johannesburg, south africa. samples of each cleaning or handling tool were prepared for aerobic (apc), coliform (cc), escherichia coli (ec), bacillus cereus (bcc), and staphylococcus aureus (sac) counts, as ...200718095448
rage: exacting a toll on the host in response to polymicrobial sepsis and listeria monocytogenes.the receptor for advanced glycation endproducts (rage) has complex roles in the immune/inflammatory response. rage is expressed on monocytes/macrophages, t and b lymphocytes, and dendritic cells. previous studies illustrated that homozygous rage-/- mice subjected to overwhelming bacterial sepsis displayed normal clearance of pathogenic bacteria and significantly increased survival. in this issue of critical care, lutterloh and colleagues confirm these findings and provide evidence that blocking ...200718190725
linezolid activity against disseminated listeria monocytogenes meningitis and central nervous system abscesses: focus on early drug myelotoxicity.a very infrequent report of listeria monocytogenes meningitis and multiple subtentorial abscesses (cerebellar, bulbar, and pontine in location), occurred in an immunocompetent man, and is described and discussed on the ground of the most recent literature evidences regarding the frequency, presentation, and outcome of this disease. the possibility to resort to combination linezolid therapy in patients who appear to be not responsive to first-line treatment of cerebral abscess of definite origin, ...200718690960
chromium in stainless steel welding fume suppresses lung defense responses against bacterial infection in rats.pulmonary infections have been reported to be increased in welders. previous animal studies have indicated that manual metal arc, stainless steel welding fume (mma-ss) increased susceptibility to lung infections. mma-ss is composed of a complex of metals (e.g., iron, chromium, nickel). the objective was to determine which metal component of mma-ss welding fume alters lung defense responses. at day 0, rats were intratracheally instilled one time with saline or mma-ss at a concentration of 2 mg/ra ...200718958720
attachment of listeria monocytogenes, escherichia coli o157:h7 and pseudomonas aeruginosa on food soiled plastic surfaces.this study investigated the ability of three different pathogens, escherichia coli o157:h7, listeria monocytogenes and pseudomonas aeruginosa, to attach food soiled (salad dressing, cooking oil, milk and yogurt) polyvinyl chloride (pvc) plastic. the pre-soiled pvc coupons were incubated with 10(8) cfu/cm2 of each bacterium in 50% tryptic soy broth for 6 h to allow attachment. it was found that the effect of food products was not significant while the effect of bacterial strain was highly signifi ...200719086561
[listeria monocytogenes infection during pregnancy--modern aspects].during a ten years period (1997-2006) at the delivery ward of maychin dom university hospital were admitted 31,430 parturients. among all of them we indentified and evaluated only 4 pregnant patients with listeria monocytogenes infection. during the first 8 years of the investigated period there was no even single case of the infection, whilst during the last 2 years the frequency of the listeria infection among all birth deliveries was 0,068%. all of our four cases were admitted with clinical s ...200718646305
occurrence of campylobacter, salmonella, yersinia enterocolitica and listeria monocytogenes in some retail food products in novi sad.the official reporting system in the province of vojvodina (pv) indicates that cases of human salmonellosis were partly covered by complete epidemiological investigation including laboratory analysis of the suspected food. intestinal campylobacteriosis and yersiniosis and four cases of septicemias caused by listeria monocytogenes were not fully epidemiologically investigated. actual country legislation on food safety does not include provisions for a routine control of the above mentioned pathog ...200718251233
[molecular characteristics of inlb protein of listeria monocytogenes wild strain and it's expression and purification].internalin b of listeria monocytogenes ta wild strain was amplified by pcr method and sequenced, then subcloned into prokaryotic vector pet28a for expression. the complete length of inlb gene was 1893 bp, encoding 630 amino acids. the front 35 amino acids consisted of signal peptide. there were one cap domain with alpha-helix region, 6 leucine-rich repeat motifs, one immunoglobulin (ig)-like domain, one b repeat sequence, 3 direct repeat gw domains and 5 n-glycosylation sites. the amino acid of ...200718271273
[synthesis and antimicrobial activity of some new (sulfon-amidophenyl)-amide derivatives of n-(m-nitrobenzoyl)-d,l-methionine].in order to obtain new compounds with potential antimicrobial activity some new (sulfon-amidophenyl)-amide derivatives of n-(m-nitrobenzoil)-d,l-methionine have been synthesized. the compounds have been obtained by decyclization reaction of 2-(m-nitrophenyl)-4-(beta-p-methylthioethyl)- delta2-5-oxazolinone with various sulfonamides. the chemical structure of the new sulfonamides was confirmed by elemental analysis data and ir and 1nmr spectral measurements. the compounds were also investigated f ...200718293716
[impact of infection with listeria monocytogenes on the pregnancy].during a ten years period (06.1998a.-06.2007) at the delivery ward of maychin dom university hospital were admitted 31 627 parturients. among all of them we indentified and evaluated only 6 pregnant patients with listeria monocytogenes infection. during the first 8 years of the investigated period there was no even single case of the infection, whilst during the last 2 years the frequency of the listeria infection among all birth deliveries was 0.10%. the first five of our cases were admitted wi ...200719705692
influence of oxygen exclusion and temperature on pathogenic bacteria levels and sensory characteristics of packed ostrich steaks throughout refrigerated storage.ostrich steaks (290) were obtained from iliofibularis muscles. for microbiological and ph determinations, samples were inoculated with listeria monocytogenes nctc 11994 (80 steaks) or escherichia coli atcc 12806 (80), then air- or vacuum-packed and stored at either 4±1°c or 10±1°c. analyses were carried out on days 0, 3, 6 and 9 of storage. for sensory evaluation, samples (130) were air- or vacuum-packed and stored at 4±1°c or at 10±1°c. sensory attributes (odour, colour, drip loss, texture and ...200722064287
the fate of two listeria monocytogenes serotypes in "cig kofte" at different storage temperatures.cig kofte is a traditional turkish food prepared from minced beef, bulgur, onions, garlic and varieties of spices. it is generally consumed within a few hours. however, leftovers can be kept in refrigerator or in room temperature up to 24h until they are consumed. in this study, survival and growth of two listeria monocytogenes serotypes were investigated in cig kofte during the storage. for this purpose, the prepared samples were separately contaminated with serotypes 1/2b or 4b of l. monocytog ...200722064198
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