Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
sensitive quantitative analysis of the meconium bacterial microbiota in healthy term infants born vaginally or by cesarean section.for decades, babies were thought to be born germ-free, but recent evidences suggest that they are already exposed to various bacteria in utero. however, the data on population levels of such pioneer gut bacteria, particularly in context to birth mode, is sparse. we herein aimed to quantify such bacteria from the meconium of 151 healthy term japanese infants born vaginally or by c-section. neonatal first meconium was obtained within 24-48 h of delivery; rna was extracted and subjected to reverse- ...201628018325
colon targeted drug delivery of branch-chained disulphide cross-linked polymers: design, synthesis, and characterisation studies.drug delivery directly to the colon is a very useful approach for treating localised colonic diseases such as inflammatory bowel disease, ulcerative colitis, and crohn's disease. the use of disulphide cross-linked polymers in colon targeted drug delivery systems has received much attention because these polymers are redox sensitive, and the disulphide bonds are only cleaved by the low redox potential environment in the colon. the goal of this study was to synthesise tricarballylic acid-based tri ...201627994641
[design of artificial foetor flatus based on bacterial volatile compounds].excessive flatulence can be a huge social problem. the purpose of this study was to design artificial flatus from bacterial volatile compounds to stimulate research into neutralizing measures.201627966414
erythritol/chlorhexidine combination reduces microbial biofilm and prevents its formation on titanium surfaces in vitro.the purpose of this in vitro study was to evaluate the antibiofilm activity of a novel air-polishing powder consisting of erythritol and chlorhexidine, assessing its ability to reduce previously grown microbial biofilm and to prevent biofilm formation on titanium surfaces.201627935124
anaerobic infections in surgical wards: a two year study.anaerobic bacteria are recognized as important pathogens in surgical infections. however, they are the most overlooked microorganisms by the clinic and the laboratory because of the tedious culture techniques with longer turn-around times. the study was aimed to analyze the frequency of anaerobic bacterial surgical infections and their predisposing factors.201627928485
improved production of 2'-fucosyllactose in engineered escherichia coli by expressing putative α-1,2-fucosyltransferase, wcfb from bacteroides fragilis.2'-fucosyllactose (2-fl) is one of most abundant oligosaccharides in human milk, which is involved in many biological functions for infant health. since 2-fl has a great potential in application to functional food materials and pharmaceuticals, several microbial systems for mass production of 2-fl have been developed in recent years. microbial production of 2-fl was suggested to be influenced by a number of factors including fucosylation activity of α-1,2-fucosyltransferase. in the present study ...201627919692
effects of a gut pathobiont in a gnotobiotic mouse model of childhood undernutrition.to model how interactions among enteropathogens and gut microbial community members contribute to undernutrition, we colonized gnotobiotic mice fed representative bangladeshi diets with sequenced bacterial strains cultured from the fecal microbiota of two 24-month-old bangladeshi children: one healthy and the other underweight. the undernourished donor's bacterial collection contained an enterotoxigenic bacteroides fragilis strain (etbf), whereas the healthy donor's bacterial collection containe ...201627881825
novel approach for evaluation of bacteroides fragilis protective role against bartonella henselae liver damage in immunocompromised murine model.bartonella henselae is a gram-negative facultative intracellular bacterium and is the causative agent of cat-scratch disease. our previous data have established that bacteroides fragilis colonization is able to prevent b. henselae damages through the polysaccharide a (psa) in an experimental murine model. in order to determine whether the psa is essential for the protection against pathogenic effects of b. henselae in immunocompromised hosts, scid mice were co-infected with b. fragilis wild type ...201627872616
bacterially-associated transcriptional remodelling in a distinct genomic subtype of colorectal cancer provides a plausible molecular basis for disease development.the relevance of specific microbial colonisation to colorectal cancer (crc) disease pathogenesis is increasingly recognised, but our understanding of possible underlying molecular mechanisms that may link colonisation to disease in vivo remains limited. here, we investigate the relationships between the most commonly studied crc-associated bacteria (enterotoxigenic bacteroides fragilis, pks+ escherichia coli, fusobacterium spp., afac+ e. coli, enterococcus faecalis & enteropathogenic e. coli) an ...201627846243
association of oncogenic bacteria with colorectal cancer in south china.to quantify fusobacterium spp., enterococcus faecalis (e.faecalis), enterotoxigenic bacteroides fragilis (etbf), and enteropathogenic escherichia coli in colorectal cancer (crc) patients and their possible association with crc clinicopathogical features, we collected the resected tumors and adjacent normal tissues (n) from 97 crc patients. 48 age- and sex-matched healthy controls (hc) were also recruited. real-time pcr was used for bacterial quantification. the median abundance offusobacterium s ...201627821805
altered gut microbiota composition associated with eczema in infants.eczema is frequently the first manifestation of an atopic diathesis and alteration in the diversity of gut microbiota has been reported in infants with eczema. to identify specific bacterial communities associated with eczema, we conducted a case-control study of 50 infants with eczema (cases) and 51 healthy infants (controls). we performed high-throughput sequencing for v3-v4 hypervariable regions of the 16s rrna genes from the gut fecal material. a total of 12,386 otus (operational taxonomic u ...201627812181
sialoglycoprotein isolated from the eggs of carassius auratus prevents bone loss: an effect associated with the regulation of gut microbiota in ovariectomized rats.the anti-osteoporotic effect of sialoglycoprotein isolated from the eggs of carassius auratus (ca-sgp) has previously been demonstrated in vivo. however, the mechanism by which this macromolecular substance regulates bone metabolism remains unclear. given the correlation between gut microbiota and the homeostasis of bone metabolism, the current study applied real-time pcr and 16s rrna high-throughput sequencing to investigate the influence of ca-sgp on gut microbiota in ovariectomized rats. real ...201627808338
pharmacokinetic and pharmacodynamic evaluation of a weight-based dosing regimen of cefoxitin for perioperative surgical prophylaxis in obese and morbidly obese patients.the objective of this study was to determine the pharmacokinetics and pharmacodynamics (pk/pd) of a weight-based cefoxitin dosing regimen for surgical prophylaxis in obese patients. patients received a single dose of cefoxitin at 40 mg/kg based on total body weight. cefoxitin samples were obtained over 3 h from serum and adipose tissue, and concentrations were determined by validated high-performance liquid chromatography. noncompartmental pharmacokinetic analysis was performed, followed by mont ...201627458209
anti-food and anti-microbial igg subclass antibodies in inflammatory bowel disease.inflammatory bowel disease (ibd), particularly crohn's disease (cd), is associated with increased microbial-specific igg and iga antibodies, whereas alterations of anti-food antibodies are still disputed. the knowledge about igg subclass antibodies in ibd is limited. in this study we analysed igg subclass antibodies specific for nutritional and commensal antigens in ibd patients and controls.201627455092
lactate dehydrogenase activity in bacteroides fragilis group strains with induced resistance to metronidazole.the aims of this study were to induce in vitro metronidazole resistance in nim-negative bacteroides fragilis group strains and to determine the lactate dehydrogenase (ldh) activity of the induced strains. a collection of b. fragilis group strains were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tof/ms). minimum inhibitory concentrations (mics) for metronidazole were determined by the agar dilution technique. the presence of nim genes was scre ...201627436459
anaerobic bacteremia: impact of inappropriate therapy on mortality.investigation on incidence and mortality of anaerobic bacteremia (ab) is clinically relevant in spite of its infrequent occurrence and not often explored, which report varies according to period and institutions. therefore, it is necessary to analyze the incidence and risk factors related to mortality and assess clinical outcomes of ab in current aspect.201627433379
the dysregulation of polyamine metabolism in colorectal cancer is associated with overexpression of c-myc and c/ebpβ rather than enterotoxigenic bacteroides fragilis infection.colorectal cancer is one of the most common cancers in the world. it is well known that the chronic inflammation can promote the progression of colorectal cancer (crc). recently, a number of studies revealed a potential association between colorectal inflammation, cancer progression, and infection caused by enterotoxigenic bacteroides fragilis (etbf). bacterial enterotoxin activates spermine oxidase (smo), which produces spermidine and h2o2 as byproducts of polyamine catabolism, which, in turn, ...201627433286
strain competition restricts colonization of an enteric pathogen and prevents colitis.the microbiota is a major source of protection against intestinal pathogens; however, the specific bacteria and underlying mechanisms involved are not well understood. as a model of this interaction, we sought to determine whether colonization of the murine host with symbiotic non-toxigenic bacteroides fragilis could limit acquisition of pathogenic enterotoxigenic b. fragilis we observed strain-specific competition with toxigenic b. fragilis, dependent upon type vi secretion, identifying an effe ...201627432285
efficient and regioselective synthesis of β-galnac/glcnac-lactose by a bifunctional transglycosylating β-n-acetylhexosaminidase from bifidobacterium bifidum.β-n-acetylhexosaminidases have attracted interest particularly for oligosaccharide synthesis, but their use remains limited by the rarity of enzyme sources , low efficiency, and relaxed regioselectivity of transglycosylation. in this work, genes of 13 β-n-acetylhexosaminidases, including 5 from bacteroides fragilis atcc 25285, 5 from clostridium perfringens atcc 13124, and 3 from bifidobacterium bifidum jcm 1254, were cloned and heterogeneously expressed in escherichia coli the resulting recombi ...201627422836
multidrug-resistant bacteroides fragilis bacteremia in a us resident: an emerging challenge.we describe a case of bacteroides fragilis bacteremia associated with paraspinal and psoas abscesses in the united states. resistance to b-lactam/b-lactamase inhibitors, carbapenems, and metronidazole was encountered despite having a recent travel history to india as the only possible risk factor for multidrug resistance. microbiological cure was achieved with linezolid, moxifloxacin, and cefoxitin.201627418986
bacteroides fragilis metabolises exopolysaccharides produced by bifidobacteria.bacteroides fragilis is the most frequent species at the human intestinal mucosal surface, it contributes to the maturation of the immune system although is also considered as an opportunistic pathogen. some bifidobacterium strains produce exopolysaccharides (eps), complex carbohydrate polymers that promote changes in the metabolism of b. fragilis when this microorganism grows in their presence. to demonstrate that b. fragilis can use eps from bifidobacteria as fermentable substrates, purified e ...201627418149
integrated pharmacokinetic-pharmacodynamic modelling to evaluate antimicrobial prophylaxis in abdominal surgery.to use monte carlo simulation with an integrated pharmacokinetic-pharmacodynamic (pk-pd) model to evaluate guideline-recommended antimicrobial prophylaxis (ap) regimens with anaerobic coverage in abdominal surgery.201627402005
a novel strain of bacteroides fragilis enhances phagocytosis and polarises m1 macrophages.commensal bacteroides fragilis possesses immune-regulatory characteristics. consequently, it has been proposed as a potential novel probiotic because of its therapeutic effects on immune imbalance, mental disorders and inflammatory diseases. macrophages play a central role in the immune response, developing either a classical-m1 or an alternative-m2 phenotype after stimulation with various signals. the interactions between macrophages and b. fragilis, however, remain to be defined. here, a new i ...201627381366
activation mechanism of the bacteroides fragilis cysteine peptidase, fragipain.enterotoxigenic bacteroides fragilis produces a secreted metalloprotease known as b. fragilis toxin (bft), which contributes to anaerobic sepsis, colitis, and colonic malignancy in mouse models of disease. a c11 family cysteine protease, fragipain (fpn), directly activates bft in the b. fragilis cell by removing the bft prodomain. fpn is itself a proenzyme and is autoactivated upon cleavage at an arginine residue in its activation loop. we have defined the proteolytic active site of fpn, demonst ...201627379832
cis-encoded small rnas, a conserved mechanism for repression of polysaccharide utilization in bacteroides.bacteroides is a major component of the human gut microbiota which has a broad impact on the development and physiology of its host and a potential role in a wide range of disease syndromes. the predominance of this genus is due in large part to expansion of paralogous gene clusters, termed polysaccharide utilization loci (puls), dedicated to the uptake and catabolism of host-derived and dietary polysaccharides. the nutritive value and availability of polysaccharides in the gut vary greatly; thu ...201627353652
genomic diversity of enterotoxigenic strains of bacteroides fragilis.enterotoxigenic (etbf) strains of bacteroides fragilis are the subset of strains that secrete a toxin called fragilysin (bft). although etbf strains are known to cause diarrheal disease and have recently been associated with colorectal cancer, they have not been well characterized. by sequencing the complete genome of four etbf strains, we found that these strains exhibit considerable variation at the genomic level. only a small number of genes that are located primarily in the bft pathogenicity ...201627348220
microbial flora of odontogenic abscesses in pet guinea pigs.abscesses of odontogenic origin in guinea pigs pose a serious health problem and need to be treated with a combination of surgical and medical therapy. the aim of this prospective study was to describe the microbial flora of odontogenic abscesses associated with osteomyelitis in 24 pet guinea pigs, to perform antibiotic sensitivity testing, and to make recommendations for practitioners on the antibiotics of first choice. inclusion criteria for the study included the animal being diagnosed with a ...201627339145
pyogenic arthritis of native joints due to bacteroides fragilis: case report and review of the literature.pyogenic arthritis of native joints due to bacteroides fragilis seems to be an infrequent disease. we analyzed the cases diagnosed in a tertiary hospital during a 22-year period and reviewed the literature to summarize the experience with this infectious entity.in our institution, of 308 patients with pyogenic arthritis of native joints, b fragilis was the causative organism in 2 (0.6%) cases. a medline search (1981-2015) identified 19 additional cases.of the 21 patients available for review (13 ...201627336895
isolation, detection, and characterization of enterotoxigenic bacteroides fragilis in clinical samples.bacteroides fragilis is an extensively studied anaerobic bacterium comprising the normal flora of the human gut. b. fragilis is known to be one of the most commonly isolated species from clinical samples and has been shown to cause a wide range of pathologies in humans [1, 2]. as an opportunistic pathogen b. fragilis can cause abscess formation and bacteremia [2]. additionally in its enterotoxigenic form, b. fragilis is a known cause of diarrheal illness, is associated with inflammatory bowel di ...201627335618
bacteroides fragilis enterotoxin upregulates heme oxygenase-1 in intestinal epithelial cells via a mitogen-activated protein kinase- and nf-κb-dependent pathway, leading to modulation of apoptosis.the bacteroides fragilis enterotoxin (bft), a virulence factor of enterotoxigenic b. fragilis (etbf), interacts with intestinal epithelial cells and can provoke signals that induce mucosal inflammation. although expression of heme oxygenase-1 (ho-1) is associated with regulation of inflammatory responses, little is known about ho-1 induction in etbf infection. this study was conducted to investigate the effect of bft on ho-1 expression in intestinal epithelial cells. stimulation of intestinal ep ...201627324483
could gut microbiota serve as prognostic biomarker associated with colorectal cancer patients' survival? a pilot study on relevant mechanism.evidences have shown that dysbiosis could promote the progression of colorectal cancer (crc). however, the association of dysbiosis and prognosis of crc is barely investigated. therefore, we used 16s rrna gene sequencing approach to determine differences in microbiota among tumor tissues of different prognosis and found that fusobacterium nucleatum and bacteroides fragilis were more abundant in worse prognosis groups, while faecalibacterium prausnitzii displayed higher abundance in survival grou ...201627323816
[postoperative necrotizing fasciitis: a rare and fatal complication].postoperative parietal complications can be exceptionally severe and serious threatening vital prognosis. necrotizing fasciitis is a rare infection of the skin and deep subcutaneous tissues, spreading along fascia and adipose tissue. it is mainly caused by group a streptococcus (streptococcus pyogenes) but also by other bacteria such as vibrio vulnificus, clostridium perfringens or bacteroides fragilis. necrotizing fasciitis is a real surgical and medical emergency. we report, in this study, a v ...201627279950
critical evaluation of ceftolozane-tazobactam for complicated urinary tract and intra-abdominal infections.the rise in resistant gram-negative pathogens continues to challenge clinicians treating infections. these resistant infections have inspired the development of new antimicrobial agents, including ceftolozane-tazobactam, a novel β-lactam/β-lactamase inhibitor combination approved by the us food and drug administration for the treatment of complicated urinary tract infections (cutis) and complicated intra-abdominal infections (ciais) in combination with metronidazole. ceftolozane exhibits bacteri ...201627279744
evaluation of vitek matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of anaerobes.rapid and adequate identification of anaerobic bacterial species still presents a challenge for most diagnostic laboratories, hindering the selection of appropriate therapy. in this study, the identification capacity of 16s rrna sequence analysis, vitek 2 (biomérieux, lyon, france) compact analysis and vitek ms-mediated identification for anaerobic bacterial species was compared. eighty-five anaerobic bacterial isolates from 11 provinces in china belonging to 14 genera were identified by these t ...201627278253
emergence and evolution of an international cluster of mdr bacteroides fragilis isolates.the aim of this study was to examine the antibiotic resistance profiles, antibiotic resistance mechanisms and possible 'clonal' nature of some mdr bacteroides fragilis strains that simultaneously harboured cfia, nimb, is1186 and is4351.201627246231
gene-microbiota interactions contribute to the pathogenesis of inflammatory bowel disease.inflammatory bowel disease (ibd) is associated with risk variants in the human genome and dysbiosis of the gut microbiome, though unifying principles for these findings remain largely undescribed. the human commensal bacteroides fragilis delivers immunomodulatory molecules to immune cells via secretion of outer membrane vesicles (omvs). we reveal that omvs require ibd-associated genes, atg16l1 and nod2, to activate a noncanonical autophagy pathway during protection from colitis. atg16l1-deficien ...201627230380
metabolic engineering of escherichia coli to produce 2'-fucosyllactose via salvage pathway of guanosine 5'-diphosphate (gdp)-l-fucose.2'-fucosyllactose (2-fl) is one of the key oligosaccharides in human milk. in the present study, the salvage guanosine 5'-diphosphate (gdp)-l-fucose biosynthetic pathway from fucose was employed in engineered escherichia coli bl21star(de3) for efficient production of 2-fl. introduction of the fkp gene coding for fucokinase/gdp-l-fucose pyrophosphorylase (fkp) from bacteroides fragilis and the fuct2 gene encoding α-1,2-fucosyltransferase from helicobacter pylori allows the engineered e. coli to p ...201627217241
characterization of crispr rna transcription by exploiting stranded metatranscriptomic data.crispr-cas systems are bacterial adaptive immune systems, each typically composed of a locus of cas genes and a crispr array of spacers flanked by repeats. processed transcripts of crispr arrays (crrnas) play important roles in the interference process mediated by these systems, guiding targeted immunity. here we developed computational approaches that allow us to characterize the expression of many crisprs in their natural environments, using community rna-seq (metatranscriptomic) data. by expl ...201627190232
screening for enterotoxigenic bacteroides fragilis in stool samples.bacteroides fragilis is a commensal bacterium found in the gut of most humans, however enterotoxigenic b. fragilis strains (etbf) have been associated with diarrhoea and colorectal cancer (crc). the purpose of this study was to establish a method of screening for the bacteroides fragilis toxin (bft) gene in stool samples, as a means of determining if carriage of etbf is detected more often in crc patients than in age-matched healthy controls. stool samples from 71 patients recently diagnosed wit ...201627166180
a proteomic approach towards understanding the cross talk between bacteroides fragilis and bifidobacterium longum in coculture.a better understanding of the interactions among intestinal microbes is needed to decipher the complex cross talk that takes place within the human gut. bacteroides and bifidobacterium genera are among the most relevant intestinal bacteria, and it has been previously reported that coculturing of these 2 microorganisms affects their survival. therefore, coculturing of bifidobacterium longum nb667 and bacteroides fragilis dsmz2151 was performed with the aim of unravelling the mechanisms involved i ...201627156738
synthesis of an aminooxy derivative of the tetrasaccharide repeating unit of streptococcus dysgalactiae 2023 polysaccharide for a ps a1 conjugate vaccine.a highly efficient and stereocontrolled synthesis of an aminooxy derivative of the tetrasaccharide repeating unit of a rhamnose-rich polysaccharide isolated from the cell envelop of bovine mastitis streptococcus dysgalactiae 2023 is reported for the first time. the synthesis was accomplished utilizing a stereoselective and convergent [2 + 2] glycosylation strategy inclusive of a disaccharide schmidt donor and an inclusive rhamnose disaccharide acceptor. the synthetic aminooxy tetrasaccharide was ...201627149417
dendritic cell-specific mgat2 knockout mice show antigen presentation defects but reveal an unexpected cd11c expression pattern.zwitterionic polysaccharide antigens such as polysaccharide a (psa) from bacteroides fragilis have been shown to activate cd4(+) t cells upon presentation by class ii major histocompatibility complex (mhcii) on professional antigen presenting cells. for t cell recognition and activation, high affinity binding between mhcii and psa is required, and complex n-glycans on conserved mhcii asparagine residues play a central role in controlling this interaction. by truncating these glycans in a myeloid ...201627146521
procarcinogenic regulatory t cells in microbial-induced colon cancer.regulatory t cell (treg) promote il-17-mediated colon tumorigenesis in multiple intestinal neoplasia (min) mice colonized with enterotoxigenic bacteroides fragilis (etbf). although they retain their immunosuppressive function, mucosal treg are instrumental to initiate etbf-triggered il-17 colitis by limiting the availability of il-2 in the local microenvironment. however, the mechanisms that trigger the recruitment of the pro-carcinogenic treg in etbf-colonized colon remained to be elucidated.201627141400
bacteroides fragilis in biopsies of patients with major abscesses and diabetic foot infections: direct molecular versus culture-based detection.direct determination by pathogen-specific real-time pcr assay for bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples. real-time pcr resulted in an increased detection rate of 12% for b. fragilis and could improve the detection of b. fragilis in clinical samples.201627112830
development of microbial trigger based oral formulation of tinidazole and its gamma scintigraphy evaluation: a promising tool against anaerobic microbes associated gi problems.tinidazole is a versatile anti-amoebic and anti-anaerobic drug used in treatment of intestinal infection. the aim of present study was to develop and evaluate a guar gum based novel target release tinidazole matrix tablet in animal models and healthy human volunteer using gamma scintigraphy technique. anti-anaerobic and anti-protozoal activity of the developed formulation was studied in vitro against bacteroides fragilis and dentamoeba fragilis. tinidazole was successful radiolabelled with (99m) ...201627108116
activation of bacteroides fragilis toxin by a novel bacterial protease contributes to anaerobic sepsis in mice.bacteroides fragilis is the leading cause of anaerobic bacteremia and sepsis. enterotoxigenic strains that produce b. fragilis toxin (bft, fragilysin) contribute to colitis and intestinal malignancy, yet are also isolated in bloodstream infection. it is not known whether these strains harbor unique genetic determinants that confer virulence in extra-intestinal disease. we demonstrate that bft contributes to sepsis in mice, and we identify a b. fragilis protease called fragipain (fpn) that is req ...201627089515
a comparative evaluation of antibacterial effectiveness of sodium hypochlorite, curcuma longa, and camellia sinensis as irrigating solutions on isolated anaerobic bacteria from infected primary teeth.in endodontics, most of the commercial intra-canal medicaments have cytotoxic reactions and because of their inability to eliminate bacteria from dentinal tubules, recent medicine has turned its attention to the usage of biologic medication prepared from natural plants. the literature to testify the efficacy of natural alternatives in primary teeth is meagre and its effects as irrigating solutions need to be evaluated.201627080968
a new chromogenic medium for isolation of bacteroides fragilis suitable for screening for strains with antimicrobial resistance.there have been an increasing number of reports describing the acquisition of antimicrobial resistance by bacteroides fragilis including the occurrence of strains with resistance to multiple antimicrobials that are relied upon for treatment of infections. the aim of this study was to design a chromogenic selective medium for isolation of b. fragilis that could be adapted for specific isolation of antimicrobial-resistant strains. bacteroides chromogenic agar (bca) was the result of this endeavour ...201627060277
[expression of enterotoxigenic bacteroides fragilis and polyketide synthase gene-expressing escherichia coli in colorectal adenoma patients].to investigate the distribution of various bacteria in adenoma tissue of colorectal adenoma (t/cra), normal colonic mucosa tissue adjacent to the adenoma (n/cra), and healthy colonic mucosa tissue (n/h) by comparing the number of total bacteria, bacteroides fragilis (bf), enterotoxigenic bacteroides fragilis (etbf), polyketide synthase (pks) gene-expressing escherichia coli(e.coli)(pks(+) e. coli)among the above 3 types of tissues.201627045721
[influence of various analgesics (pethidine, pentazocine and piritramide) on respiration and blood circulation]. 20165050524
queen anne's favourite physician, john arbuthnot, m.d. (1667-1735). 20164860040
deputizing services. 20164714493
[luhacovice and the fate of janácek]. 20164936053
half nose. 20164718450
patient-specific bacteroides genome variants in pouchitis.a 2-year longitudinal microbiome study of 22 patients who underwent colectomy with an ileal pouch anal anastomosis detected significant increases in distinct populations of bacteroides during 9 of 11 patient visits that coincided with inflammation (pouchitis). oligotyping and metagenomic short-read annotation identified bacteroides populations that occurred in early samples, bloomed during inflammation, and reappeared after antibiotic treatment. targeted cultivation of bacteroides isolates from ...201627935837
role of enterotoxigenic bacteroides fragilis in children less than 5 years of age with diarrhea in tabriz, iran.diarrhea is the most frequent health problem among children in developing countries. defining the etiology of acute diarrhea is critical to disease therapy and prevention. some anaerobic bacteria such as enterotoxigenic bacteroides fragilis (etbf) strains cause diarrheal disease by production of enterotoxin in children less than 5 years old.201627635209
comparative genomic analysis identifies structural features of crispr-cas systems in riemerella anatipestifer.riemerella anatipestifer infection is a contagious disease that has resulted in major economic losses in the duck industry worldwide. this study attempted to characterize crispr-cas systems in the disease-causing agent, riemerella anatipestifer (r. anatipestifer). the crispr-cas system provides adaptive immunity against foreign genetic elements in prokaryotes and crispr-cas loci extensively exist in the genomes of archaea and bacteria. however, the structure characteristics of r. anatipestifer c ...201627577199
identification of a new lipoprotein export signal in gram-negative bacteria.bacteria of the phylum bacteroidetes, including commensal organisms and opportunistic pathogens, harbor abundant surface-exposed multiprotein membrane complexes (sus-like systems) involved in carbohydrate acquisition. these complexes have been mostly linked to commensalism, and in some instances, they have also been shown to play a role in pathogenesis. sus-like systems are mainly composed of lipoproteins anchored to the outer membrane and facing the external milieu. this lipoprotein localizatio ...201627795390
dna inversion regulates outer membrane vesicle production in bacteroides fragilis.phase changes in bacteroides fragilis, a member of the human colonic microbiota, mediate variations in a vast array of cell surface molecules, such as capsular polysaccharides and outer membrane proteins through dna inversion. the results of the present study show that outer membrane vesicle (omv) formation in this anaerobe is also controlled by dna inversions at two distantly localized promoters, ivp-i and ivp-ii that are associated with extracellular polysaccharide biosynthesis and the express ...201626859882
tissue-associated bacterial alterations in rectal carcinoma patients revealed by 16s rrna community profiling.sporadic and inflammatory forms of colorectal cancer (crc) account for more than 80% of cases. recent publications have shown mechanistic evidence for the involvement of gut bacteria in the development of both crc-forms. whereas, colon and rectal cancer have been routinely studied together as crc, increasing evidence show these to be distinct diseases. also, the common use of fecal samples to study microbial communities may reflect disease state but possibly not the tumor microenvironment. we pe ...201628018861
high occurrence of fusobacterium nucleatum and clostridium difficile in the intestinal microbiota of colorectal carcinoma patients.colorectal carcinoma is considered the fourth leading cause of cancer deaths worldwide. several microorganisms have been associated with carcinogenesis, including enterococcus spp., helicobacter pylori, enterotoxigenic bacteroides fragilis, pathogenic e. coli strains and oral fusobacterium. here we qualitatively and quantitatively evaluated the presence of oral and intestinal microorganisms in the fecal microbiota of colorectal cancer patients and healthy controls. seventeen patients (between 49 ...201626691472
mycotic aneurysm of the common carotid artery as a presenting symptom for early colorectal malignancy.mycotic carotid aneurysms are rare vascular conditions that are associated with high mortality if left untreated. we present a gentleman who had a progressively enlarging tender pulsatile swelling on the right side of neck. emergency computed tomography scan showed a large mycotic aneurysm arising from the right common carotid artery, and he underwent emergency open exploration with interposition bypass graft. bacteroides fragilis species, which signified gastrointestinal-related sepsis, was iso ...201626363427
latent infections as a risk factor for posttrabeculectomy bleb failure.to investigate latent conjunctival chlamydia trachomatis (ct) and bacteroides fragilis (bf) infections as potential risk factors for posttrabeculectomy bleb failure.201625651208
effects of gatifloxaine content in gatifloxacine-loaded plga and β-tricalcium phosphate composites on efficacy in treating osteomyelitis.composites of gatifloxacin (gflx)-loaded poly (lactic-co-glycolic) acid (plga) and β-tricalcium phosphate (βtcp) containing 0, 1, and 10 wt % gflx (0, 1, and 10 wt % gflx composites), and gflx-loaded plga containing 1, 5, and 10 wt % gflx (1, 5, and 10wt % gflx-plga) as controls were fabricated and characterized in vitro and in vivo. on in vitro evaluation, the 10 wt % gflx composite released gflx over at least 28 days in hanks' balanced solution and exhibited clinically sufficient bactericidal ...201625533357
an in vitro experimental study on the antimicrobial activity of silicone oil against anaerobic bacteria.to investigate the in vitro antimicrobial activity of silicone oil against anaerobic agents, specifically propionibacterium acnes, peptostreptococcus spp., peptostreptococcus anaerobius, bacteroides fragilis, fuobacterium spp., and clostridium tertium.201625356916
prevalence and characterization of enterotoxigenic bacteroides fragilis and toxigenic clostridium difficile in a taipei emergency department.enterotoxigenic bacteroides fragilis (etbf) and toxin-encoding clostridium difficile (txcd) are associated with gastroenteritis. routine anaerobic blood culture for recovery of these anaerobic pathogens is not used for the detection of their toxins, especially for toxin-variant txcd. the aim of this study was to investigate the prevalence and risk factors of the genotypes of these anaerobes in patients with acute diarrheal illnesses.201725648668
nim gene-independent metronidazole-resistant bacteroides fragilis in surgical site infections.background:bacteroides fragilis is the most common anaerobic pathogen isolated from surgical site infections (ssis). metronidazole resistance is increasing and the mechanisms of resistance are not clear in some isolates. the aim of the present study was to investigate the metronidazole susceptibility prevalence, and detect nim genes in b. fragilis isolates from ssis. methods: this study included 100 surgery patients with signs and symptoms indicative of ssis. syringe aspiration of the infected s ...201728840093
septic abortion presenting as a right lower trapezius abscess secondary to bacteroides fragilis bacteraemia.a 43-year-old japanese woman was evaluated in the outpatient department for right shoulder pain and fever, which began 5 days earlier. mri of the right shoulder revealed a high-intensity area deep in the right trapezius muscle. aspiration revealed purulent fluid, and gram staining of the fluid showed gram-negative bacilli. the patient was also found to be profoundly anaemic and to have a positive urine pregnancy test. on admission, we initiated intravenous ampicillin-sulbactam and aztreonam. she ...201728827296
ontogenesis of the gut microbiota composition in healthy, full-term, vaginally born and breast-fed infants over the first 3 years of life: a quantitative bird's-eye view.early-life intestinal microbiota development is crucial for host's long-term health and is influenced by many factors including gestational age, birth and feeding modes, birth environment, ethnic/geographical background, etc. however, 'quantitative' data on the actual population levels of gut bacterial communities when these influences are controlled for is relatively rare. herein, we demonstrate a quantitative perspective of microbiota development in natural and healthy milieus, i.e., in health ...201728785253
determination of the mutation prevention concentration of imipenem for the two imipenem susceptible bacteroides fragilis strains q1f2 (cfia-positive) and atcc 25282 (cfia-negative). 201728782706
a prospective randomised trial of isolated pathogens of surgical site infections (ssi).every surgical wound is colonized by bacteria, but only a small percentage displays symptoms of infection. the distribution of pathogens isolated in surgical site infections has not significantly changed over the last decades. staph. aureus, coag(-) staphylococci, enterococcus spp and e. coli are the main strains appearing. in addition, a continuously rising proportion of surgical site infections caused by resistant bacterial species (mrsa, c. albicans) has been reported.201728761643
detection of enterotoxin and protease genes among hungarian clinical bacteroides fragilis isolates.bacteroides fragilis as a commensal bacterium is a member of the human intestinal flora, but as an opportunistic pathogen it can cause serious infections as well. some of them, harbouring an enterotoxin gene (bft), may cause diarrhoea mainly in young children. recently it has been shown that a member of c11 proteases called fragipain (fpn) can activate the enterotoxin, while c10 protease (bfp) is suspected of playing an important role in the invasiveness of the b. fragilis isolates. the objectiv ...201728754475
anaerobic bacteria growth in the presence of cathelicidin ll-37 and selected ceragenins delivered as magnetic nanoparticles cargo.cationic antibacterial peptides (caps) and synthetic molecules mimicking the amphiphilic structure of caps, such as ceragenins, are promising compounds for the development of new antimicrobials.201728747178
secretory products of the human gi tract microbiome and their potential impact on alzheimer's disease (ad): detection of lipopolysaccharide (lps) in ad hippocampus.although the potential contribution of the human gastrointestinal (gi) tract microbiome to human health, aging, and disease is becoming increasingly acknowledged, the molecular mechanics and signaling pathways of just how this is accomplished is not well-understood. major bacterial species of the gi tract, such as the abundant gram-negative bacilli bacteroides fragilis (b. fragilis) and escherichia coli (e. coli), secrete a remarkably complex array of pro-inflammatory neurotoxins which, when rel ...201728744452
the association of type ii diabetes with gut microbiota composition.it is known that type 2 diabetes (t2d) in humans could be linked to the composition of gut microbiota. the aim of this study was to evaluate three faecal bacterial species, including bacteroides fragilis, bifidobacterium longum and faecalibacterium prausnitzii in patients with t2d. this case control study included 18 patients with t2d and 18 matched persons without diabetes. the concentrations of b. fragilis, b. longum and f. prausnitzii were determined by quantitative real-time pcr. quantitativ ...201728739439
orthopedic infections caused by obligatory anaerobic gram-negative rods: report of two cases.anaerobic bone and joint infections are uncommon, although the number of anaerobic infections is presumably underestimated because of difficulties with isolation and identification of obligate anaerobes. this study describes two cases of complicated bacteroides fragilis peri-implant infection of the lumbar spine, infection of the hip and osteomyelitis. bacteria were identified with the use of a mass spectrometer, vitek ms system. drug susceptibility was performed with the use of e-test. the euca ...201728730548
case of xanthogranulomatous oophoritis.xanthogranulomatous inflammation is characterized by destruction of the tissues of the organ involved and replacement by chronic inflammatory cells such as lymphocytes, plasma cells, occasional neutrophils with or without multinucleated or touton giant cells. exact aetiology is not known but the theory of infection with organisms like proteus, e coli, and bacteroides fragilis is most popular. xanthogranulomatous inflammation of the female genital tract is not common and usually involves the endo ...201728712201
effect of soy isoflavones on growth of representative bacterial species from the human gut.the present work aimed to assess the susceptibility of dominant and representative bacterial populations from the human gut to isoflavones and their metabolites. to do so, the minimum inhibitory concentration (mic) of isoflavone glycosides, isoflavone aglycones, and equol to 37 bacterial strains was determined by broth microdilution. additionally, for 10 representative strains, growth curves, growth rate (μ), and optical density (od600 nm) of the cultures at 24 h were also determined. mics of da ...201728698467
outer membrane vesicles secreted by pathogenic and nonpathogenic bacteroides fragilis represent different metabolic activities.numerous studies are devoted to the intestinal microbiota and intercellular communication maintaining homeostasis. in this regard, vesicles secreted by bacteria represent one of the most popular topics for research. for example, the outer membrane vesicles (omvs) of bacteroides fragilis play an important nutritional role with respect to other microorganisms and promote anti-inflammatory effects on immune cells. however, toxigenic b. fragilis (etbf) contributes to bowel disease, even causing colo ...201728694488
bacteroides fragilis enterotoxin induces the formation of autophagosomes in endothelial cells but interferes their autophagosomal fusion with lysosomes for complete autophagic flux through a mitogen-activated protein kinase, ap-1, and c/ebp homologous protein-dependent pathway.bacteroides fragilis enterotoxin (bft), a virulence factor of enterotoxigenic b. fragilis (etbf), plays an essential role in mucosal inflammation. although autophagy contributes to the pathogenesis of diverse infectious diseases, little is known about autophagy in etbf infection. this study was conducted to investigate the role of bft in the autophagic process in endothelial cells (ecs). stimulation of human umbilical vein ecs (huvecs) with bft increased lc3-ii conversion from lc3-i and protein ...201728694294
reactivity toward bifidobacterium longum and enterococcus hirae demonstrate robust cd8(+) t cell response and better prognosis in hbv-related hepatocellular carcinoma.recent studies suggest that several bacterial species are involved in tumor immunosurveillance and antitumor immunity. the role of bacteria in immune responses in hbv-related hepatocellular carcinoma (hcc) patients is still unknown. in this study, we examined the bacteria-reactive cd8(+) t cell response in patients with hbv-related hcc. we found that circulating cd8(+) t cells from healthy individuals demonstrated minimal or zero specificity toward a series of commensals and bacteria previously ...201728694023
phosphonodifluoropyruvate is a mechanism-based inhibitor of phosphonopyruvate decarboxylase from bacteroides fragilis.bacteroides fragilis, a human pathogen, helps in the formation of intra-abdominal abscesses and is involved in 90% of anaerobic peritoneal infections. phosphonopyruvate decarboxylase (pnpdc), a thiamin diphosphate (thdp)-dependent enzyme, plays a key role in the formation of 2-aminoethylphosphonate, a component of the cell wall of b. fragilis. as such pnpdc is a possible target for therapeutic intervention in this, and other phosphonate producing organisms. however, the enzyme is of more general ...201728693916
gut microbiota in health, diverticular disease, irritable bowel syndrome, and inflammatory bowel diseases: time for microbial marker of gastrointestinal disorders?few data exist on differences in gut microbiota composition among principal gastrointestinal (gi) diseases. we evaluated the differences in gut microbiota composition among uncomplicated diverticular disease (dd), irritable bowel syndrome (ibs) and inflammatory bowel diseases (ibd) patients. dd, ibs, and ibd patients along with healthy controls (ct) were enrolled in our italian gi outpatient clinic. stool samples were collected. microbiota composition was evaluated through a metagenomic gene-tar ...201728683448
tlr2 and interleukin-10 are involved in bacteroides fragilis-mediated prevention of dss-induced colitis in gnotobiotic mice.bacteroides fragilis (bf) are gram-negative anaerobe symbionts present in the colon. recent studies have reported the beneficial role of bf in maintaining intestinal homeostasis, stimulating host immunologic development, and preventing infectious colitis caused by pathogenic bacteria. our previous studies showed that monocolonization of germ-free mice with bf significantly reduced colon inflammations and damage.201728683149
oral il-10 suppresses colon carcinogenesis via elimination of pathogeniccd4(+) t-cells and induction of antitumor cd8(+) t-cell activity.an oral sustained-release formulation of interleukin-10 suppressed tumor growth and enhanced survival in the apc(min/+)/bacteroides fragilis spontaneous colon cancer model. therapeutic benefit was associated with a 5-fold reduction in cd4(+)rorγt(+)foxp3(-)il-17(+) t-helper cell, cd4(+)rorγt(+)foxp3(+)il-17(+) pathogenic t-regulatory cell and cd4(+)rorγt(-)foxp3(+)il-17(-) conventional t-regulatory cell numbers and a concurrent 2-fold enhancement in cd8(+) t-cell activity in the colon. selective ...201728680752
corrigendum: interaction of bacteroides fragilis toxin with outer membrane vesicles reveals new mechanism of its secretion and delivery.[this corrects the article on p. 2 in vol. 7, pmid: 28144586.].201728670574
extracellular vesicles in gastrointestinal cancer in conjunction with microbiota: on the border of kingdoms.extracellular vesicle (ev) production is a universal feature of metazoan cells as well as prokaryotes (bmvs - bacterial microvesicles). they are small vesicles with phospholipid membrane carrying proteins, dna and different classes of rnas and are heavily involved in intercellular communication acting as vectors of information to target cells. for the last decade, the interest in ev research has exponentially increased though thorough studies of their roles in various pathologies that was not pr ...201728669749
prevalences of antimicrobial resistance and the cfia resistance gene in danish bacteroides fragilis group isolates since 1973.the purpose of this study was to determine the prevalences of resistance and the cfia carbapenemase-producing gene in historical bacteroides fragilis group isolates.201728666749
the bacteroides fragilis pathogenicity island links virulence and strain competition.the mature microbiome is a stable ecosystem that resists perturbation despite constant host exposure to exogenous microbes. however, the microbial mechanisms determining microbiome development and composition are poorly understood. we recently demonstrated that a non-toxigenic b. fragilis (ntbf) strain restricts enteric colonization by an enterotoxigenic (etbf) strain dependent on a type vi secretion system (t6ss). we show here that a second enterotoxigenic strain is competent to colonize, depen ...201728632016
in vitro/ in vivo evaluation of the efficacy of gatifloxacine-loaded plga and hydroxyapatite composite for treating osteomyelitis.molten 10 wt% gatifloxacine (glfx-loaded poly(lactide-co-glycolide) (plga) was introduced into three-dimensionally interconnected pores and onto the surfaces of hydroxyapatite (ha) granules. the composite granules exhibited clinically sufficient bactericidal activities against streptococcus milleri and bacteroides fragilis from 3 h to 10 days. the composite granules were implanted in bone defects created by debridement of osteomyelitis lesions in rabbit mandibles. after 4-week implantation, infl ...201728626206
the effects of the lactobacillus casei strain on obesity in children: a pilot study.there are few data regarding the role of probiotics as a dietary intervention in the management of obesity in children. an open prospective examination was conducted to clarify the effects of lactobacillus casei strain shirota (lcs)-containing beverages in obese children. we compared the intestinal microbiota and organic acid levels between 12 obese (average age, 10.8 years; body mass index (bmi) z score, 2.7±1.7) and 22 control children(average age, 8.5 years; bmi z score, 0.1±0.7), and pre- an ...201728618860
tlr2-stimulating contaminants in glycoconjugate fractions prepared from bacteroides fragilis.bacteroides fragilis is a member of the normal intestinal flora and is involved in host immunostimulation via tlr2. on the bacterial cell surface, glycoconjugates, such as lps and capsular polysaccharide a (psa), have been reported to participate in host immunostimulation via tlr2. previously, we identified a tlr2-stimulating lipoprotein in b. fragilis cells. in this study, we demonstrated that tlr2-stimulating principal molecules in glycoconjugate fractions prepared from b. fragilis are contami ...201728606014
implementation of the new virtuo blood culture system: evaluation and comparison to the 3d system using simulated blood cultures.to evaluate the performances of the newly approved bact/alert virtuo blood culture system for the recovery of bloodstream pathogens and compare it to the bact/alert 3d system.201728583022
bioluminescence imaging to track bacteroides fragilis inhibition of vibrio parahaemolyticus infection in mice.bacteroides fragilis is an anaerobic, gram-negative, commensal bacterium of the human gut. it plays an important role in promoting the maturation of the immune system, as well as suppressing abnormal inflammation. many recent studies have focused on the relationship between b. fragilis and human immunity, and indicate that b. fragilis has many useful probiotic effects. as inhibition of intestinal pathogens is an important characteristic of probiotic strains, this study examined whether b. fragil ...201728553617
association between yogurt consumption and intestinal microbiota in healthy young adults differs by host gender.human intestinal microbiota are influenced by various factors viz. diet, environment, age, gender, geographical, and socioeconomic situation, etc. among which diet has the most profound impact. however, studies investigating this impact have mostly included subjects from diverse geographic/socioeconomic backgrounds and hence the precise effects of dietary factors on gut microbiota composition remain largely confounded. herein, with an aim to evaluate the association between dietary habits, speci ...201728553274
nimh, a novel nitroimidazole resistance gene contributing to metronidazole resistance in bacteroides fragilis. 201728535278
the sialate o-acetylesterase esta from gut bacteroidetes species enables sialidase-mediated cross-species foraging of 9-o-acetylated sialoglycans.the gut harbors many symbiotic, commensal, and pathogenic microbes that break down and metabolize host carbohydrates. sialic acids are prominent outermost carbohydrates on host glycoproteins called mucins and protect underlying glycan chains from enzymatic degradation. sialidases produced by some members of the colonic microbiota can promote the expansion of several potential pathogens (e.g. clostridium difficile, salmonella, and escherichia coli) that do not produce sialidases. o-acetyl ester m ...201728526748
gut dysbiosis following c-section instigates higher colonisation of toxigenic clostridium perfringens in infants.herein we investigated the intestinal carriage of α-toxigenic and enterotoxigenic clostridium perfringens during infancy, focusing on its association with other gut microbes and mode of delivery and feeding. faecal samples from 89 healthy term infants were collected at age 7 days, 1 month, 3 months, 6 months and 3 years. c. perfringens was quantified by qpcr; other gut bacteria were quantified by reverse-transcription-qpcr. alpha-toxigenic c. perfringens was detected in 3.4% infants at day 7 but ...201728504574
optimization of cord blood unit sterility testing: impact of dilution, analysis delay, and inhibitory substances.different methods are used by cord blood banks to prepare samples for sterility testing. suboptimal methods can result in the release of contaminated products. in our organization, samples are prepared by diluting the final product in rpmi-1640 medium. in this work, we have compared our method with different approaches to verify whether optimization should be sought.201728474347
[effect of silver nanoparticles on anaerobic bacteria].the aim was to evaluate the antibacterial effect of silver nanoparticles on anaerobic bacteria.201728467592
Refresh
firstprev
...919293949596979899100...
nextlast
Displaying items 9901 - 10000 of 10047