Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| diversity of feline chlamydia psittaci revealed by random amplification of polymorphic dna. | dna samples from c. psittaci including 6 strains of feline origin, 10 strains of avian origin, 1 strain of ovine origin and 1 strain of guinea pig origin were amplified each with three 10-nucleotide (nt) primers and four > 18-nt primers. amplified products were separated by polyacrylamide gel electrophoresis. eight patterns were recognized by random amplification of polymorphic dna (rapd) fingerprinting of c. psittaci: 2 patterns of feline origin, 5 patterns of avian origin and 1 pattern of guin ... | 1997 | 9050172 |
| the prevalence of chlamydia psittaci infections in belgian commercial turkey poults. | the prevalence of chlamydia psittaci infections in belgian commercial turkey poults was examined and a follow-up study of one belgian turkey flock was performed. sera were examined for the presence of anti-chlamydia antibodies by immunoblotting. cloacal and conjunctival swab smears and lung impression smears were examined for the presence of chlamydial antigen using the imagen chlamydia immunofluorescence test. anti-chlamydia antibodies were found in 90 of 100 sera collected at slaughter from tu ... | 1997 | 9050173 |
| secretion of proinflammatory cytokines by epithelial cells in response to chlamydia infection suggests a central role for epithelial cells in chlamydial pathogenesis. | chlamydia species infect epithelial cells at mucosal surfaces, and are major causes of sexually transmitted diseases. infection is characterized by inflammation which is exacerbated upon reinfection, ultimately leading to tissue damage and scarring. although central for the development of disease manifestations, little is known about the mechanisms that initiate and sustain the inflammatory response to chlamydia. infection of cervical and colonic epithelial cells with chlamydia trachomatis and c ... | 1997 | 9011579 |
| in vitro and in vivo antichlamydial activities of hsr-903, a new fluoroquinolone antibiotic. | the in vitro and in vivo antichlamydial activities of hsr-903 were investigated. the mics of hsr-903 for different species of chlamydia were 0.016 to 0.063 microg/ml, which were superior to those of conventional fluoroquinolones. the therapeutic effect of hsr-903 in experimental mouse chlamydia psittaci pneumonia was also excellent and almost equal to that of minocycline and superior to that of ofloxacin. these results indicate that hsr-903 may be useful in the treatment of respiratory infection ... | 1997 | 9087506 |
| [community acquired pneumonia. reliability of the criteria for deciding ambulatory treatment]. | to determine whether criteria for not admitting community-acquired pneumonia (cap) patients diagnosed in the emergency room are appropriate, and to characterize the symptoms, etiology and course of cap. this one-year prospective, protocol study of immunocompetent cap patients diagnosed in the emergency room of our hospital enrolled patients not considered to require hospital admission according to the recommendations of the spanish society of respiratory disease (separ). medical histories, chest ... | 1997 | 9091117 |
| [significance of causes of infectious abortion in sheep flocks in northern baden-württemberg with special reference to chlamydia psittaci]. | investigations on the reasons of infectious abortion cases in sheep flocks in northern parts of baden-wuerttemberg eludicate the wide-spreading of chlamydia psittaci (c. psittaci) and its significance as the most frequent cause of abortions in sheep. another important pathogen agent causing abortions is salmonella abortus ovis (s. abortus ovis) which could be demonstrated by using a serological elisa test. a less important role than c. psittaci and s. abortus ovis plays coxiella burnetii in abor ... | 1997 | 9092464 |
| psittacosis. | pneumonia caused by chlamydia psittaci likely occurs considerably more frequently than is suggested by the mere 100 to 200 cases reported annually to public health authorities. parrots and other psittacine birds still are regarded as the major reservoir of the infectious agent and most recognized cases are associated with owning pet birds or working in a pet store. it was once thought that birds imported from abroad, often illegally, were a principal source, but many domestic breeder flocks of p ... | 1997 | 9097370 |
| phylogenetic analysis of the genus chlamydia based on 16s rrna gene sequences. | the phylogenetic relationships among chlamydia spp. were investigated by comparing 16s rrna gene sequences. in this analysis we used 14 strains of chlamydia psittaci, including seven feline isolates, two avian isolates, two human isolates, one bovine isolates, one ovine isolate, and one koala isolate; and nine strains of chlamydia trachomatis, including six human isolates, two swine isolates, and one mouse isolate. a phylogenetic analysis of the 16s rrna gene sequences of these organisms and sev ... | 1997 | 9103632 |
| the ribosomal intergenic spacer and domain i of the 23s rrna gene are phylogenetic markers for chlamydia spp. | current methods used to classify chlamydia strains, including biological, morphological, and dna hybridization techniques and major outer membrane protein (omp1) gene analysis, can be imprecise or difficult to perform. to facilitate classification, 2.8-kb partial ribosomal dna (rdna) segments from a chlamydia trachomatis strain and a chlamydia psittaci strain were amplified by pcr and sequenced. subsequently, a 1,320-bp region in this segment, including both the 16s/23s intergenic spacer (232 +/ ... | 1997 | 9103637 |
| comparison of chlamydia psittaci from cats with upper respiratory tract disease by polymerase chain reaction analysis of the ompa gene. | conjunctival swabs were taken from 168 cats with clinical signs of acute or chronic upper respiratory tract disease and tested for chlamydia psittaci by the polymerase chain reaction (pcr) to amplify the ompa gene coding region. twenty-two (13 per cent) were positive for c psittaci. the pcr products from positive samples were subjected to restriction endonuclease analysis with the restriction enzymes alu i and mse i. the fragments of dna were detected on silver-stained polyacrylamide gels and th ... | 1997 | 9106964 |
| myopericarditis due to chlamydia psittaci. the role of autoimmunity. | chlamydia psittaci may rarely involve the heart as the only manifestation of the infection. we report a case of myopericarditis caused by this agent, whose pathogenesis seemed to be autoimmune damage triggered by the infection. | 1997 | 9112307 |
| species identification of chlamydia isolates by analyzing restriction fragment length polymorphism of the 16s-23s rrna spacer region. | the genetic diversity of the 16s-23s rrna spacer region of 12 chlamydia pneumoniae isolates, 7 chlamydia trachomatis isolates (human biovars: the trachoma serovars b and c, the urogenital serovars d, e, and f, and the lymphogranuloma venereum serovar l2; and a mouse biovar), 6 chlamydia psittaci isolates (5 avian isolates and 1 feline isolate), and one chlamydia pecorum isolate was studied. the 16s-23s rrna spacer region was amplified by pcr and digested with the restriction enzymes msei, psti, ... | 1997 | 9114403 |
| use of monoclonal antibodies to facilitate identification, cloning, and purification of chlamydia trachomatis hsp10. | as a requisite for a physiological and immunological investigation, reagents were developed that facilitated the identification and purification of chlamydia trachomatis hsp10 (chsp10). monoclonal antibodies that specifically recognize chsp10 were generated with multiple-antigen peptides (maps) to promote recognition of chlamydia-specific epitopes. map2, containing amino acids 54 to 69 of the hsp10 sequence, elicited strong antibody responses after immunization of balb/c mice. monoclonal antibod ... | 1997 | 9114409 |
| serological survey of chlamydial antibody in post-natal sera. | questionnaires (3426; 72.8%) and blood samples (3890; 82.6%) were analysed from 4704 women post-natally. the answers to the questionnaire confirmed that those patients living in areas with the highest percentage employed in agriculture were most likely to be in direct or indirect contact with sheep or sheep products. when the 788 (20.3%) of the sera with group specific antibody were examined for type-specific antibody, 291 (7.5%) reacted with chlamydia trachomatis, 153 (3.9%) with chlamydia pneu ... | 1997 | 9459402 |
| serological diagnosis of chlamydial abortion in sheep and goats: comparison of the complement fixation test and an enzyme-linked immunosorbent assay employing solubilised proteins as antigen. | a new elisa for antibodies against chlamydial abortion of ewes which uses detergent solubilised proteins (dsp) of chlamydia psittaci as antigen (anderson, i.e., herring, a.j., jones, g.e., low, j.c., greig, a., 1995. development and evaluation of an indirect elisa to detect antibodies to abortion strains of chlamydia psittaci in sheep sera. vet. microbiol., 43, pp. 1-12] was compared with the complement fixation test (cft) in screening 1000 ovine and caprine sera obtained from selected flocks/he ... | 1997 | 9460194 |
| [occurrence of antibodies to chlamydial group antigens in the population of the czech republic]. | a set of 4,178 human blood sera was tested including 3,093 samples collected from blood donors, 624 randomly collected samples. 124 samples collected from persons possibly exposed to chlamydia infection, and 337 samples from patients of various hospitals in the districts of brno, olomouc and rumburk. all the sera were examined using the complement fixation microtest and the antigen of chlamydia psittaci for cfr (bioveta, ivanovice na hané, czr). moreover, a subset of samples collected from selec ... | 1997 | 9162449 |
| localization by immunoelectron microscopy of antigens of chlamydia psittaci suitable for diagnosis or vaccine development. | two different antigens of serotype 1 chlamydia psittaci were localized using three immunoelectron microscopy techniques: non-embedding, pre-embedding and post-embedding. the antigens had previously been described as being of potential use in diagnosis (80-90 kda protein region) and vaccine development (110 kda protein). the results show a direct relationship between the protective capacity of the antigens and their surface localization on the elementary bodies, which are the infectious form of c ... | 1997 | 9163915 |
| distribution of endosomal, lysosomal, and major histocompatability complex markers in a monocytic cell line infected with chlamydia psittaci. | the intracellular fate of chlamydia psittaci during infection of a monocytic cell line, thp1, was characterized. cytochalasin d inhibited phagocytosis of latex beads but had no effect on infection by c. psittaci, and vacuoles expressed the transferrin receptor, suggesting accessibility to the endocytic pathway. early chlamydia-containing vacuoles expressed major histocompatibility complex (mhc) class i molecules, and most vacuoles fused with host cell lysosomes, since they expressed lamp-1 and h ... | 1997 | 9169786 |
| in vitro and in vivo activities of am-1155, a new fluoroquinolone, against chlamydia spp. | the in vitro and in vivo activities of am-1155, a new quinolone, against chlamydia spp. were investigated. the mic of am-1155 for 10 standard strains of different chlamydia spp. and 25 wild-type strains of chlamydia pneumoniae isolated in japan, which were morphologically different from clinical isolates from the united states, ranged from 0.063 to 0.125 microg/ml. its activity was almost the same as those of sparfloxacin and tosufloxacin and was 4 and 16 times superior to those of levofloxacin ... | 1997 | 9174194 |
| infective endocarditis due to unusual or fastidious microorganisms. | infective endocarditis due to fastidious microorganisms is commonly encountered in clinical practice. some organisms such as fungi account for up to 15% of cases of prosthetic valve infective endocarditis, whereas organisms of the hacek group (haemophilus parainfluenzae, h. aphrophilus, and h. paraphrophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella kingae) cause 3% of community-acquired cases of infective endocarditis. special techniques a ... | 1997 | 9179137 |
| gestational psittacosis: case report and literature review. | in europe, chlamydia psittaci is a relatively common cause of abortion in sheep and other mammals. psittacosis in humans is typically described as a mild-to-moderate flu-like illness. if psittacosis is acquired during pregnancy, it can present as a severe, progressive, febrile illness, with headache, disseminated intravascular coagulation, abnormal liver enzyme studies, and impaired renal function. only cases with significant fetomaternal morbidity and mortality have been reported. recovery from ... | 1997 | 9195579 |
| polymerase chain reaction (pcr) detection of porcine chlamydia trachomatis and ruminant chlamydia psittaci serovar 1 dna in formalin-fixed intestinal specimens from swine. | in previous studies chlamydiae were detected immunohistologically in the gut of 66 out of 311 pigs. the aim of the present investigation was the classification of these intestinal porcine chlamydiae. for the study, dna extracted from 52 paraffin-embedded intestinal tissues was amplified in nested polymerase chain reactions (pcrs) with chlamydia omp1 genus- and species-specific primers. some of the amplification products were cloned and sequenced. in 45 cases dna could be amplified with genus-spe ... | 1997 | 9197211 |
| some characteristics of a smooth type lipopolysaccharide of chlamydia psittaci. | 1997 | 9199717 | |
| chlamydiosis in captive white-winged doves (zenaida asiatica). | chlamydia psittaci was isolated from the spleen of a moribund white-winged dove (zenaida asiatica). the isolate was serotyped as the serovar b that is commonly isolated from pigeons. a fourfold increase in the titer of antichlamydial igm activity occurred in that bird in paired serum samples tested by chlamydial elementary body agglutination (eba) and a greater than or equal to fourfold decrease of igg occurred by direct complement fixation (dcf). the increases or decreases of eba and dcf titers ... | 1997 | 9201425 |
| plasmid diversity in chlamydia. | chlamydiae exhibit low interspecies dna homology and plasmids from different chlamydial species can be readily distinguished by southern blot analysis and restriction enzyme profiling. in contrast, available plasmid sequence data from within the species chlamydia trachomatis indicate that plasmids from human isolates are highly conserved. to evaluate the nature and extent of plasmid variation, the complete nucleotide sequences were determined for novel plasmids from three diverse non-human chlam ... | 1997 | 9202459 |
| gestational psittacosis in a montana sheep rancher. | in humans, psittacosis is primarily a flulike illness following exposure to psittacine birds. in rare cases, pregnant women exposed to chlamydia psittaci can contract gestational psittacosis: atypical pneumonia, sepsis, and placental insufficiency resulting in premature birth or miscarriage. in the united states, only two cases of gestational psittacosis have been reported, both from exposure to psittacine birds. eleven other cases have been reported worldwide, mostly in the united kingdom, all ... | 1997 | 9204302 |
| [community-acquired pneumonia--current status of pathogen diagnosis]. | procedures for the microbiological diagnosis of acute community-acquired pneumonia are based on the expected pathogens. although a great variety of microorganisms are able to cause community-acquired pneumonia only a few pathogens play an important role in daily practice. the most important investigations are blood cultures and sputum cultures to detect bacteria like pneumococci, haemophilus influenzae and staphylococcus aureus as well as antibody tests for mycoplasma pneumonia and chlamydia pne ... | 1997 | 9206930 |
| two new serovars of chlamydia psittaci from north american birds. | five chlamydia psittaci isolates (1 turkey, 1 psittacine, 1 human, and 2 pigeon isolates) failed to react with serovar-specific monoclonal antibodies to known avian and mammalian c. psittaci serovars and were presumed to represent 1 or more new serovars. the isolates were characterized using restriction endonuclease analysis of the whole genome, polymerase chain reaction-restriction fragment length polymorphism of the major outer membrane protein genome, monoclonal antibody comparisons, and grow ... | 1997 | 9211235 |
| application of a nested, multiplex pcr to psittacosis outbreaks. | we developed a nested, multiplex pcr for simultaneous detection of three species of chlamydiae in human and avian specimens. the pcr was designed to increase sensitivity and to circumvent inhibitors of pcr present in clinical specimens. the target sequence was the 16s rrna gene. the first-step pcr was genus specific, and the second-step pcr was multiplexed (i.e., had multiple primer sets in the same tube) and could discriminate among chlamydia pneumoniae, chlamydia psittaci, and chlamydia tracho ... | 1997 | 9230378 |
| isolation of faecal chlamydia from sheep in britain and their characterization by cultural properties. | faeces samples were taken per rectum from sheep on 26 farms in england and wales and examined for the presence of chlamydia by culture in mccoy cell monolayers. thirteen of the farms were known to have had abortion outbreaks associated with chlamydia psittaci (enzootic abortion) and 13 were free of this infection. the chlamydia isolated were characterized by cultural techniques. chlamydia were isolated from the faeces of lambs on all 26 farms and the prevalence of infection varied form 5-50% on ... | 1997 | 9232119 |
| compendium of psittacosis (chlamydiosis) control, 1997. centers for disease control and prevention. | infection with chlamydia psittaci, often referred to as avian chlamydiosis (ac), is an important cause of systemic illness in companion birds (i.e., birds kept by humans as pets) and poultry. infection can be transmitted from infected birds to humans. in humans, infection caused by c. psittaci is referred to as psittacosis, which can result in fatal pneumonia. this compendium provides information on ac (also known as psittacosis, ornithosis, and parrot fever) and psittacosis (also known as parro ... | 1997 | 9233554 |
| [chlamydia psittaci and chlamydia pneumoniae infection in caceres]. | the study of the clinical and epidemiological characteristics of infectious process caused by chlamydia pneumoniae and chlamydia psittaci in our medium, cáceres. | 1997 | 9235099 |
| zoonotic and viral infection in fetal loss after 12 weeks. | one hundred and thirty-six women from an urban, rural and farming community were recruited to a study of infectious causes of midtrimester miscarriage (n = 85), stillbirth (n = 32), or termination for developmental (n = 17) or chromosomal (n = 2) abnormalities. no woman had evidence of acute infection with toxoplasma, listeria, leptospira or chlamydia psittaci (ovine enzootic abortion). one woman had midtrimester miscarriage associated with primary cytomegolovirus infection and five women had ev ... | 1997 | 9255087 |
| use of a live chlamydial vaccine to prevent ovine enzootic abortion. | a lyophilised chlamydial vaccine was prepared from the 1b temperature-sensitive strain of ovine chlamydia psittaci. ewes inoculated with a low titre of the live vaccine four weeks before artificial insemination were challenged on day 70 of gestation with five uk field isolates of c psittaci, including strains a22 and s26/3 previously incorporated into a commercial inactivated vaccine. there was a significantly lower chlamydial abortion rate after challenge in the vaccinated group (7.1 per cent) ... | 1997 | 9257434 |
| respiratory chlamydial infections in a hong kong teaching hospital and association with coronary heart disease. | the microimmunofluorescence antibody technique was used to determine the seroprevalence of antibodies to chlamydia pneumoniae and chlamydia psittaci from hospitalized patients in hong kong. antibodies to c. pneumoniae were found in 54.8% of 157 patients hospitalized with respiratory disease, in 71.1% of 83 patients with cardiac disease and in 31.2% of 93 patients without either respiratory or cardiac disease. antibodies to c. psittaci were found in 0.9% of the combined study groups. there was se ... | 1997 | 9259078 |
| the effects of crude placental extract and erythritol on growth of chlamydia psittaci (ovis) in mccoy cells. | 1997 | 9266662 | |
| use of synthetic antigens improves detection by enzyme-linked immunosorbent assay of antibodies against abortigenic chlamydia psittaci in ruminants. | synthetic peptide antigens were prepared for use in enzyme-linked immunosorbent assays (elisas) to detect serum antibodies against abortigenic strains of chlamydia psittaci in livestock. peptide antigens were identified with c. psittaci b577-immune sera by solid-phase scanning of overlapping octapeptides of variable domains (vds) of the major outer membrane protein of c. psittaci serovar 1 (omp1 type c. psittaci b577). two vd 4 regions and one vd 2 region were strongly reactive with all c. psitt ... | 1997 | 9276405 |
| type iii secretion genes identify a putative virulence locus of chlamydia. | four genes of chlamydia psittaci strain guinea pig inclusion conjunctivitis (gpic), whose predicted products are highly homologous to structural and regulatory components of a contact-dependent or type iii secretion apparatus, were isolated. related to genes present in several animal and plant bacterial pathogens, these genes may represent a section of a previously undetected chromosomal virulence locus analogous to several recently described virulence-associated type iii secretion loci. the exi ... | 1997 | 9282747 |
| a case of relapsing psittacosis associated with a stroke. | psittacosis, caused by chlamydia psittaci, is mainly associated with an atypical pneumonia. we report a case of psittacosis where onset of respiratory symptoms was soon followed by the onset of focal neurological signs, a very uncommon feature. despite adequate treatment with erythromycin, the patient relapsed when re-exposed to a sick pet bird. a prolonged course of doxycycline led to resolution of the pneumonia, but it is important to recall that successful management must also include the eli ... | 1997 | 9287275 |
| comparison of the omp i gene of chlamydia psittaci between isolates in victorian koalas and other animal species. | the objective of this study is to compare the strain of chlamydia causing genital infection in koalas from victoria with isolates from other animal species. | 1997 | 9293340 |
| chlamydia psittaci infection in sheep: a paradigm for human reproductive tract infection. | chlamydiae are important reproductive tract pathogens in a wide variety of animals. in humans, chronic or repeated infection of the female genital tract with chlamydia trachomatis has been identified as a significant factor in the development of occlusive infertility or increased risk of ectopic pregnancy. the spectrum of reproductive disease recognized in sheep to be caused by chlamydia psittaci has been primarily restricted to pregnant animals because the organism was clearly identified as a m ... | 1997 | 9350636 |
| phylogenetic analyses of chlamydia psittaci strains from birds based on 16s rrna gene sequence. | the nucleotide sequences of 16s ribosomal dna (rdna) were determined for 39 strains of chlamydia psittaci (34 from birds and 5 from mammals) and for 4 chlamydia pecorum strains. the sequences were compared phylogenetically with the gene sequences of nine chlamydia strains (covering four species of the genus) retrieved from nucleotide databases. in the neighbor-joining tree, c. psittaci strains were more closely related to each other than to the other chlamydia species, although a feline pneumoni ... | 1997 | 9350757 |
| evaluation of a new commercial microimmunofluorescence test for detection of antibodies to chlamydia pneumoniae, chlamydia trachomatis, and chlamydia psittaci. | a new commercial test for chlamydial serology, the mrl-micro-immunofluorescent test (mrl; mrl diagnostics, usa) was compared with the standard microimmunofluorescence test (mif) using sera from 246 patients. chlamydia pneumoniae immunoglobulin g (igg) antibodies were detected in 46.3% (mif) and 64.2% (mrl) of sera and chlamydia trachomatis igg in 23.2% (mif) and 25.2% (mrl); chlamydia psittaci igg antibodies were found with the mrl in 1% of the sera from a general population and in 17.3% of pres ... | 1997 | 9352264 |
| neurological complications of chlamydial infections: case report and review. | we describe a patient with chlamydia pneumoniae infection who presented with cerebellar dysfunction, followed by respiratory failure requiring mechanical ventilation. c. pneumoniae is an important respiratory pathogen, and other clinical manifestations, including neurological syndromes, are being increasingly recognized. meningoencephalitis and other neurological complications have also been described in patients with infections due to chlamydia psittaci and chlamydia trachomatis. chlamydial inf ... | 1997 | 9356800 |
| 'farmer's ear': sudden sensorineural hearing loss due to chlamydia psittaci infection. | a case of sudden sensorineural hearing loss in association with a chlamydia psittaci pneumonia is reported. rapid recovery was seen when the patient was treated with high dose steroids and appropriate antibiotics. this is the first such case report in the literature. | 1997 | 9373553 |
| epizootic of chlamydia psittaci infection in goats in taiwan. | epizootic abortion in goats has been frequently reported in taiwan since 1993. the outbroken flocks were found in most of districts in taiwan. no apparent clinical signs were found in aborted doe. the typical abortion occurred in the last two months of pregnancy. the incidence of abortion was from 10% to 87% in outbroken farms in 1993, and a total of 976 out of 2130 pregnancies (46%) were found abortion in our investigation. gross lesions in aborted fetuses included generalized haemorrhage and s ... | 1997 | 9373948 |
| chlamydiae in porcine abortion. | formalin-fixed, paraffin-embedded fetal livers and lungs from 139 cases of swine abortion were investigated retrospectively for chlamydiae by means of immunohistochemistry. using a genus-specific antibody, chlamydial antigen was found in eight livers obtained from five (3.6%) abortion cases from different herds. all lung sections were negative. chlamydiae were also labeled in five of the eight positive livers using a monoclonal antibody against immunotype 1 of chlamydia psittaci; the remaining t ... | 1997 | 9381659 |
| in vitro activities of doxycycline and enrofloxacin against european chlamydia psittaci strains from turkeys. | the in vitro susceptibility of 14 european chlamydia psittaci strains from turkeys to the antibiotics doxycycline and enrofloxacin was tested. for doxycycline the mic ranged from 0.05 to 0.2 microg/ml, with an average of 0.1 microg/ml. for enrofloxacin the mic was 0.25 microg/ml. acquired resistance was not detected against doxycycline and enrofloxacin. | 1997 | 9420065 |
| two-dimensional electrophoretic analysis of the protein family at 90 kda of abortifacient chlamydia psittaci. | four major clusters, designated a, b, c and d, were distinguished in western blots by a monoclonal antibody specific for the "antigen family at 90 kda" after two-dimensional electrophoretic analysis on immobilized ph gradient of chlamydial elementary bodies of abortifacient c. psittaci. clusters b, c, and d were closely related with molecular mass (kda) pi values of 91.5/5.2-5.4, 90/5.0-5.2 and 90.5/5.6-5.8, respectively. cluster a was larger, with molecular mass/pi of 104.7/5.1-5.3. evidence fo ... | 1997 | 9420176 |
| immunocytologic detection of chlamydia psittaci from cervical and vaginal samples of chronically infected ewes. | an immunocytologic method was developed for the detection of chronic chlamydia psittaci infection from the reproductive tract of ewes. vaginal and cervical samples from 8 infected and 2 non-infected ewes were stained with a c. psittaci-specific monoclonal antibody. cells containing c. psittaci were only detected from the 8 infected ewes and the level of detection varied with respect to the estrus cycle. an increased number of infected cells were observed during the periovulation period, thus ind ... | 1998 | 9442944 |
| characterization of a chlamydia psittaci dna binding protein (euo) synthesized during the early and middle phases of the developmental cycle. | the euo gene (for early upstream open reading frame) of chlamydia psittaci was previously found to be transcribed better at 1 than at 24 h postinfection. we found that the euo gene encodes a minor protein that is expressed within 1 h of infection of host cells with c. psittaci 6bc but that protein quantity peaks during the logarithmic growth phase of reticulate bodies (rbs), declines late in the infection (after 20 h) when rbs reorganize into elementary bodies (ebs), and is absent in infectious ... | 1998 | 9488410 |
| enhancement of atp levels and glucose metabolism during an infection by chlamydia. nmr studies of living cells. | the chlamydia species are obligate intracellular bacteria that proliferate only within the infected cell. since the extracellular bacteria are metabolically inert and there are no cell-free systems for characterizing chlamydia metabolism, we studied metabolic changes related to atp synthesis and glycolysis in hela cells infected with chlamydia psittaci during the course of the 2-day infection cycle using noninvasive 31p and 13c nmr methods. we find that the infection stimulates atp synthesis in ... | 1998 | 9507014 |
| a search for chlamydia psittaci in products of conception. | 1998 | 9515693 | |
| molecular cloning and characterization of the genes coding for the highly immunogenic cluster of 90-kilodalton envelope proteins from the chlamydia psittaci subtype that causes abortion in sheep. | proteins present in the outer membrane of chlamydiae that are involved in mucosal epithelial cell infection must clearly be identified and characterized if we are to understand and modify the pathogenic mechanisms utilized by these organisms. we have identified and isolated a family of four genes encoding putative outer membrane proteins (pomps), a group of proteins of approximately 90 kda present in the outer membrane of the subtype of chlamydia psittaci that causes ovine enzootic abortion (str ... | 1998 | 9529048 |
| human mannose-binding protein inhibits infection of hela cells by chlamydia trachomatis. | the role that collectin (mannose-binding protein) may play in the host's defense against chlamydial infection was investigated. recombinant human mannose-binding protein was used in the inhibition of cell culture infection by chlamydia trachomatis (c/tw-3/ot, e/uw-5/cx, and l2/434/bu), chlamydia pneumoniae (ar-39), and chlamydia psittaci (6bc). mannose-binding protein (mbp) inhibited infection of all chlamydial strains by at least 50% at 0.098 microg/ml for tw-3 and uw-5, and at 6.25 microg/ml f ... | 1998 | 9529088 |
| the sinorhizobium meliloti mucr protein, which is essential for the production of high-molecular-weight succinoglycan exopolysaccharide, binds to short dna regions upstream of exoh and exoy. | sinorhizobium meliloti (rhizobium meliloti) is able to produce two different exopolysaccharides, succinoglycan and galactoglucan. mutations in the mucr gene of s. meliloti result in the stimulation of galactoglucan synthesis, while the type of succinoglycan produced is modified. in culture supernatants of a mucr mutant, low-molecular-weight succinoglycan is present, whereas no high-molecular-weight succinoglycan could be detected. the biosynthesis of succinoglycan is directed by the products of ... | 1998 | 9529524 |
| mucosal and systemic humoral immune response of turkeys after infection and reinfection with a chlamydia psittaci serovar d strain. | the purpose of this study was to examine the effects of chlamydia-specific antibodies in tears and tracheal washings (iga and igg) and sera (igg) on chlamydial excretion during the course of an experimental infection and reinfection of turkeys with chlamydia psittaci. two groups of turkeys were experimentally infected with a serovar d strain of chlamydia psittaci, either at the age of 7 days or at the age of 35 days. a third group was infected at the age of 7 days and reinfected with the same st ... | 1998 | 9533081 |
| development of the male urogenital system of the koala phascolarctos cinereus. | this paper described several developmental stages of the male urogenital system in the koala phascolarctos cinereus, employing both light and scanning electron microscopy. there are few studies of the development of the urogenital system in male marsupials. findings by white and timms (1994) that male koalas can be infected with chlamydia psittaci emphasise the importance of studies on male animals and in particular their reproductive system. specimens in our study ranged in age from 15 days pos ... | 1998 | 9543340 |
| characterization of the systemic disease and ocular signs induced by experimental infection with chlamydia psittaci in cats. | in addition to the commonly reported ocular signs, chlamydia psittaci infection of kittens resulted in fever, lethargy, lameness and reduction in weight gain following ocular instillation of virulent organisms. the appearance of these systemic signs was late with respect to the appearance of ocular symptoms and occurred simultaneously with increasing levels of chlamydia-specific igg. measurement of acute phase reactants and il-6 in plasma indicated that both became elevated concurrent with or sl ... | 1998 | 9556858 |
| experimental genital tract infection with chlamydia psittaci (gpic agent) in male rats. | the course of experimental chlamydial infection of the male genital tract was studied. inoculation of the chlamydia psittaci agent of guinea pig inclusion conjunctivitis (gpic agent) into the vas deferens of rats resulted in chlamydial infection of the epididymis, testis and the prostate gland. the inflammatory response was most prominent at 14 days after infection. chlamydiae were recovered from the epididymides and the prostate glands for up to 90 and 60 days post inoculation, respectively. hi ... | 1998 | 9561385 |
| internalization of chlamydia by dendritic cells and stimulation of chlamydia-specific t cells. | chlamydia species are the causative agents of trachoma, various forms of pneumonia, and the most common sexually transmitted diseases. although the infection cycle has been extensively characterized in epithelial cells, where the chlamydia entry-vacuoles avoid fusion with host-cell lysosomes, the cellular immune response has received less attention. moreover, despite the abundant presence of dendritic cells (dc) in the sites of infection, the interaction between chlamydia and dc has never been s ... | 1998 | 9570547 |
| kinetics of infection and effects on placental cell populations in a murine model of chlamydia psittaci-induced abortion. | the anatomical progression of chlamydial infection was studied in different areas of the placenta, using a mouse model and two inoculation times: early pregnancy (day 7, group a) and midpregnancy (day 11, group b). the first population cells affected were decidual cells and neutrophils located just at the limits of the maternal and fetal placenta. the following invaded area was the layer of giant cells. complete colonization of the maternal placenta occurred after day 15 of pregnancy independent ... | 1998 | 9573099 |
| serotyping of chlamydia psittaci isolates from ratites. | 1998 | 9576350 | |
| infection with feline chlamydia psittaci. | 1998 | 9577527 | |
| [early manifestation of septic shock and disseminated intravascular coagulation complicated by acute myocardial infarction in a patient suspected of having legionnaires' disease]. | legionellosis is an important cause of severe pneumonia in the community. inadequate therapy will lead to respiratory distress syndrome, disseminated intravascular coagulation (dic) and finally fatal multiple organ failure. we encountered a rare case in which early manifestation included septic shock and dic complicated by acute myocardial infarction (ami) suspected to be derived from legionnaires' disease. a 54-year-old healthy female complained of lumbago, high fever and dry cough 10 days afte ... | 1998 | 9585703 |
| cytokines and the protective host immune response to chlamydia psittaci. | the immunobiology of enzootic abortion of ewes (eae) is incompletely understood. the causative agent is chlamydia psittaci, which infects many ruminant species and has zoonotic potential. the organism can survive in the ovine host for many months without causing clinical symptoms but does not generate a sterile immunity during this time. it has been postulated that the organism persists in the host entering at a latent phase, possibly mediated by host cytokine production. the effects of cytokine ... | 1998 | 9597304 |
| analysis of the humoral immune response to chlamydia outer membrane protein 2. | the humoral immune response to chlamydia outer membrane protein 2 (omp2) was studied. omp2 is a highly genus-conserved structural protein of all chlamydia species, containing a variable n-terminal fragment. to analyze where the immunogenic parts were localized, seven highly purified truncated fusion proteins constituting different regions of the protein were produced (chlamydia pneumoniae-omp2aa23-aa93, chlamydia psittaci-omp2aa23-aa94, and chlamydia trachomatis-omp2aa23-aa84, aa87-aa547, aa23-a ... | 1998 | 9605983 |
| chronic follicular conjunctivitis associated with chlamydia psittaci or chlamydia pneumoniae. | we determined whether patients with chronic conjunctivitis in whom direct fluorescent antibody (dfa) tests revealed genus-specific chlamydial antigens (but not species-specific chlamydia trachomatis antigens) were infected with chlamydia psittaci or chlamydia pneumoniae. patients were divided into a case group of possible non-trachomatis chlamydial conjunctivitis and a control group of nonchlamydial conjunctivitis on the basis of examination and dfa testing. species-specific primers were used to ... | 1998 | 9636859 |
| detection of chlamydiosis in a shipment of pet birds, leading to recognition of an outbreak of clinically mild psittacosis in humans. | avian chlamydiosis was detected in a shipment of > 700 pet birds from a florida bird distributor that were sold to nine atlanta-area pet stores in august 1995. respiratory illness among persons who had recently acquired birds from this shipment was reported to local public health officials. the attack rate of acute respiratory illness was 10.7% among persons in households exposed to birds from the implicated flock vs. 1.8% among control households (odds ratio, 6.60; 95% confidence interval, 1.39 ... | 1998 | 9636874 |
| [detection of chlamydia psittaci infections in amphibians using an immunofluorescence test]. | in amphibians, chlamydia psittaci infections are known since 1982 as a fatal disease. only documented in two amphibian species, the pathogen seems to be widespread in these coldblooded vertebrates. the detection of intracytoplasmatic inclusion bodies and following examinations (electronmicroscopy) were necessary for the postmortal diagnosis. in 26 amphibians (3 species) a specific immunological testkit (immunofluerescence test) have been used. in 18 cases a positive result was found. this is the ... | 1998 | 9639956 |
| susceptibility of alpine ibex to conjunctivitis caused by inoculation of a sheep-strain of mycoplasma conjunctivae. | we evaluated the susceptibility of alpine ibex (capra ibex ibex) to mycoplasmal conjunctivitis induced by a strain of mycoplasma conjunctivae isolated from domestic sheep by inoculation of three alpine ibexes with 1.2 x 10(6) colony forming units of m. conjunctivae in the conjunctival sac of both eyes. one more ibex was exposed to the infection by contact. experimental animals were free of m. conjunctivae and ocular chlamydia infection before inoculation. conjunctivitis and serous to mucous lach ... | 1998 | 9646477 |
| tandem genes of chlamydia psittaci that encode proteins localized to the inclusion membrane. | chlamydiae are obligate intracellular bacteria that replicate within a non-acidified vacuole, termed an inclusion. to identify chlamydial proteins that are unique to the intracellular phase of the life cycle, a lambda expression library of chlamydia psittaci dna was differentially screened with convalescent antisera from infected guinea pigs and antisera directed at formalin-fixed purified chlamydial elementary bodies (ebs). one library clone was identified that harboured two open reading frames ... | 1998 | 9663687 |
| compendium of measures to control chlamydia psittaci infection among humans (psittacosis) and pet birds (avian chlamydiosis), 1998. center for disease control and prevention. | psittacosis -- also known as parrot disease, parrot fever, and ornithosiscan cause severe pneumonia and other serious health problems among humans. approximately 800 cases of psittacosis (infection with chlamydia psittaci) were reported to cdc from 1987 through 1996, and most resulted from exposure to pet birds, usually parrots, macaws, cockatiels, and parakeets. in birds, c. psittaci infection is referred to as avian chlamydiosis (ac). infected birds shed the bacteria through feces and nasal di ... | 1998 | 9671426 |
| characterization of a neutralizing monoclonal antibody directed at the lipopolysaccharide of chlamydia pneumoniae. | identification of protective epitopes is one of the first steps in the development of a subunit vaccine. one approach to accomplishing this is to identify structures or epitopes by using monoclonal antibodies (mab) that can attenuate infectivity in vitro and in vivo. to date attempts to use this approach with chlamydia pneumoniae have failed. this report is the first description of a mab directed to the lipopolysaccharide (lps) of chlamydia that neutralizes both in vitro and in vivo the infectiv ... | 1998 | 9673271 |
| immunoelectron microscopic localisation of the omp90 family on the outer membrane surface of chlamydia psittaci. | the putative outer membrane location of the omp90 (formerly pomp) family from the ovine abortion strain of chlamydia psittaci was investigated by immunoelectron microscopy. using a non-embedding technique, antigens were shown to be localised on the outer membrane surface of both elementary and reticulate bodies, the infectious and non-infectious forms of chlamydiae respectively. antibodies affinity-purified against the expressed amino- and carboxy-terminal halves of one of the family members. om ... | 1998 | 9675857 |
| isolation of an avian serovar of chlamydia psittaci from a case of bovine abortion. | 1998 | 9683079 | |
| recent advances on ovine chlamydial abortion. | this paper reviews new findings on ovine chlamydial abortion. concerning chlamydial taxonomy, with the recent advances due to the analysis of the ribosomal genes, nine genotypic groups were identified separated into two lineages. it also describes the transmission of the disease, the site of entry of the organism and chlamydial shedding by underlying the role of latent infections. recent results also concern the pathogenesis, with the kinetics of placental colonization and placental pathology le ... | 1998 | 9689742 |
| [psittacosis (chlamydiosis) of birds and the necessity of government disease control]. | psittacosis--induced by chlamydia psittaci--occurs at present as individual cases but not any more as an endemic or epidemic disease. the diagnosis does not pose unsurmountable difficulties. the dominant sources of infection for men are--unlike in previous times--now budgerigars and cockatiels from domestic breeding sources but also--as far as it is known at all--different domestic mammals. a targeted therapy of diseases in humans and birds is successfully possible with chlortetracycline, doxycy ... | 1998 | 9710938 |
| abortion in guinea pigs by chlamydia psittaci isolates from natural sheep abortion. | experimental model for chlamydial abortion was planned using pregnant guinea pigs to study whether the isolate of chlamydia psittaci from natural cases of sheep abortion is able to cause experimental abortion in pregnant guinea pigs or not. follow up and clinical observations like thermal reaction and haematological changes exhibited biphaic febrile response and marked leucopenia in both intraperitoneal group as well as intravaginal group. the laboratory examinations revealed presence of chlamyd ... | 1998 | 9717453 |
| a comparison of dna amplification, isolation and serology for the detection of chlamydia psittaci infection in cats. | chlamydia psittaci is a significant cause of conjunctivitis in cats, but can be difficult to diagnose owing to the small number of organisms in conjunctival swabs. in the united kingdom laboratory diagnosis is based on three techniques: isolation of the infectious organism, amplification of chlamydial dna by the polymerase chain reaction (pcr) or the detection of anti-chlamydial antibodies by immunofluorescence assay. to determine the most sensitive method these techniques were compared in the f ... | 1998 | 9725174 |
| induction of apoptosis by chlamydia psittaci and chlamydia trachomatis infection in tissue culture cells. | the role of programmed cell death (apoptosis) in llc-mk2 cells infected with chlamydia trachomatis lgv2 serotype and chlamydia psittaci 6bc strain was investigated using flow cytometry and tunel procedures. the number of apoptotic cells was significantly higher at 72 and 96 hours post infection in the chlamydia infected cell cultures in comparison with mock-infected cells. we postulate the apoptotic process to be a mechanism induced by c. trachomatis and c. psittaci infection in llc-mk2 cells. | 1998 | 9728403 |
| the intracellular life of chlamydia psittaci: how do the bacteria interact with the host cell? | throughout the life of any organism interactions with the surrounding environment are always taking place, a process that leads to evolution. chlamydia psittaci is an obligate intracellular parasite, but it must also be capable of extracellular survival in order to search for new host cells. therefore, these peculiar prokaryotes have evolved two different particles and a unique developmental cycle that, together with a series of not yet fully understood interactions with their host cells, allow ... | 1998 | 9729764 |
| community outbreak of psittacosis in a rural australian town. | health authorities in victoria, australia were notified of three men from a rural town with atypical pneumonia, admitted to hospital over 8 days. initial serological testing suggested chlamydia psittaci as the cause. we did a case-control study to find risk factors for psittacosis. | 1998 | 9734887 |
| [severe pneumonia with a pneumococcal aspect during an ornithosis outbreak]. | to describe the clinical, radiological and biological features of chlamydia psittaci pneumonia. | 1998 | 9767806 |
| apoptosis of epithelial cells and macrophages due to infection with the obligate intracellular pathogen chlamydia psittaci. | we have characterized the cytotoxic activity of the obligate intracellular bacterium chlamydia psittaci, which resides within a membrane-bound vacuole during the 2-day infection cycle. we have established that infected epithelial cells and macrophages die through apoptosis, which is measurable within 1 day of infection and requires productive infection by the bacteria. inhibition of host cell protein synthesis has no effect on cell death, but blocking bacterial entry or bacterial protein synthes ... | 1998 | 9780196 |
| [community acquired pneumonia requiring admission to hospital. etiology and follow-up of 366 cases]. | an eighteen-month prospective study designed to determine the incidence, etiology and prognosis of community acquired pneumonia (cap) in adults requiring admission to hospital. | 1998 | 9780423 |
| relationship between abortions and seroprevalences to selected infectious agents in dairy cows. | the aim of this study was to determine whether the occurrence of abortion is related to the seroprevalence of abortion-causing infectious agents. in a cross-sectional study, cattle from dairy farms in switzerland that were defined as having an abortion problem were divided into two groups: cows with a history of abortion within the previous 3 months (cases) and cows without a history of abortion (controls). a positive titre to leptospira spp. was associated with an increased probability of being ... | 1998 | 9780831 |
| the major outer membrane protein of chlamydia psittaci functions as a porin-like ion channel. | the major outer membrane protein (momp) of chlamydia species shares several biochemical properties with classical porin proteins. secondary structure analysis by circular dichroism now reveals that momp purified from chlamydia psittaci has a predominantly beta-sheet content (62%), which is also typical of bacterial porins. can momp form functional ion channels? to directly test the "porin channel" hypothesis at the molecular level, the momp was reconstituted into planar lipid bilayers, where it ... | 1998 | 9784523 |
| high-level expression of chlamydia psittaci major outer membrane protein in cos cells and in skeletal muscles of turkeys. | the omp1 genes encoding the major outer membrane proteins (momps) of avian chlamydia psittaci serovar a and d strains were cloned and sequenced. the nucleotide sequences of the avian c. psittaci serovar a and d momp genes were found to be 98.9 and 87.8% identical, respectively, to that of the avian c. psittaci serovar a strain 6bc, 84.6 and 99.8% identical to that of the avian c. psittaci serovar d strain nj1, 79.1 and 81.1% identical to that of the c. psittaci guinea pig inclusion conjunctiviti ... | 1998 | 9784562 |
| experimental chlamydia psittaci serotype 1 enteric infection in gnotobiotic piglets: histopathological, immunohistochemical and microbiological findings. | the enteric pathogenicity of the ovine c. psittaci serotype 1 isolate s26/3 was assessed using a litter of gnotobiotic piglets. in one group, eight piglets were inoculated at 3 days of age; at 10 days, two of these were re-inoculated. in a second group, six animals were mock-inoculated at 3 days of age as negative controls; subsequently, at 10 days, three of these piglets were inoculated with c. psittaci. the animals were observed for clinical signs, killed and necropsied sequentially between 4 ... | 1998 | 9791872 |
| pcr detection of chlamydia psittaci in faecal samples from passerine birds in sweden. | to investigate to what extent wild passerine birds are carriers of chlamydia psittaci, 312 faecal samples from 18 bird species were collected. using the pcr technique and subsequent dna sequencing, c. psittaci dna was demonstrated in faecal samples from 9 (2.9%) birds of 6 different species. sera from 65 bird-ringers, highly exposed to wild birds, were tested by microimmunofluorescence assay for the occurrence of igg and igm antibodies to c. psittaci. no such antibodies were found. this results ... | 1998 | 9825803 |
| chlamydia trachomatis inca is localized to the inclusion membrane and is recognized by antisera from infected humans and primates. | chlamydia psittaci produces a collection of proteins, termed inca, incb, and incc, that are localized to the chlamydial inclusion membrane. in this report we demonstrate that inca is also produced by chlamydia trachomatis. c. trachomatis inca is structurally similar to c. psittaci inca and is also localized to the inclusion membrane. immunoblot analysis demonstrated that sera from c. trachomatis-infected patients and from experimentally infected monkeys both recognized c. trachomatis inca. | 1998 | 9826388 |
| [evaluation of serum kl-6 levels in summer-type hypersensitivity pneumonitis]. | a high level of serum kl-6 is a known feature of active pulmonary fibrosis. some researchers have suggested that kl-6 is produced and secreted by type ii pneumocytes. the present study evaluated serum kl-6 levels in patients with summer-type hypersensitivity pneumonitis (summer-type hp) (n = 6, 7 episodes), mycoplasma pneumoniae pneumonia (n = 16), chlamydia psittaci pneumonia (n = 3), chlamydia pneumoniae pneumonia (n = 9), and bacterial pneumonia (n = 12). in addition, transbronchial lung biop ... | 1998 | 9866978 |
| aflp allows the identification of genomic markers of ruminant chlamydia psittaci strains useful for typing and epidemiological studies. | amplified fragment length polymorphism (aflp), a novel method for molecular typing, was evaluated for its ability to differentiate among a group of highly related chlamydia psittaci strains isolated from ruminants and belonging to serotype 1. a total set of 12 strains were included in this study, 10 strains inducing abortion in ruminants and 2 strains from faecal samples. for the aflp analysis, the total purified genomic dna of each strain was submitted to a one-step digestion-ligation reaction ... | 1998 | 9921580 |
| [a very serious course of psittacosis in pregnancy]. | a 33-year-old pregnant woman was admitted because of atypical pneumonia. she developed adult respiratory distress syndrome complicated by premature birth and perinatal mortality. the diagnosis 'psittacosis' was established on clinical grounds and confirmed serologically (complement fixation test). she was treated with doxycycline 200 mg i.v. daily for 3 weeks and was discharged in good condition after three months. indirect case history revealed infected birds from the neighbours as the likely s ... | 1998 | 10028357 |
| the production of eae-free lambs from infected dams using multiple ovulation and embryo transfer. | this investigation aimed to ascertain whether embryo transfer was a feasible method of breaking the disease cycle caused by chlamydia psittaci (ovis). ten naive ewe lambs were inoculated orally with the t76 and g188 isolates of c. psittaci (ovis) in late pregnancy. five animals which sero-converted to the complement fixation test (cft) were used as donors for a multiple ovulation and embryo transfer programme. three ewes excreted chlamydiae at parturition 1 year after inoculation, with one anima ... | 1998 | 9455162 |
| application of polymerase chain reaction for detection of legionella pneumophila in serum samples. | objective: to apply the polymerase chain reaction (pcr) to serum samples for the rapid diagnosis of legionnaire's disease using the l5sl9 and l5sr93 primers designed to generate a 104-base-pair (bp) fragment from the 5s rna gene of legionella spp. the amplified product was detected by electrophoresis and by hybridization with the l5s-1-specific probe. methods: single specimens of serum obtained from 24 patients with confirmed legionellosis, at different stages of their disease, were tested by pc ... | 1998 | 11864308 |
| the chlamydial pneumonias. | chlamydia psittaci and chlamydia pneumoniae are important causes of community-acquired pneumonias. less commonly, c. trachomatis may cause pneumonia in adult immunocompromised hosts but more commonly is responsible for pneumonia in neonates. c. psittaci is the cause of psittacosis and is the only chlamydial zoonotic atypical pneumonia. c. pneumoniae is being increasingly recognized as the cause of up to 10% of community-acquired pneumonias. c. pneumoniae pneumonia has a clinical presentation lik ... | 1998 | 14743268 |
| evaluation of the polymerase chain reaction in comparison with other diagnostic methods for the detection of chlamydia psittaci. | various diagnostic methods exist for the detection of chlamydia psittaci. in the current study, the test performance of polymerase chain reaction (pcr) was compared with other testing methods used in the diagnosis of c. psittaci. tissue and fecal specimens (n = 119) of avian and mammalian origin were tested by pcr and one or more of the following methods: cell culture, enzyme-linked immunosorbent assay, and direct fluorescein-conjugated monoclonal antibody staining. several gold standards, based ... | 1999 | 12968730 |
| review of techniques for the diagnosis of chlamydia psittaci infection in psittacine birds. | 1999 | 12968739 |