Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| biosorption of cadmium(ii), lead (ii) and copper(ii) with the filamentous fungus phanerochaete chrysosporium. | the biosorption from artificial wastewaters of heavy metals (cd(ii), pb(ii) and cu(ii)) onto the dry fungal biomass of phanerochaete chryosporium was studied in the concentration range of 5-500 mg l(-1). the maximum absorption of different heavy metal ions on the fungal biomass was obtained at ph 6.0 and the biosorption equilibrium was established after about 6 h. the experimental biosorption data for cd(ii), pb(ii) and cu(ii) ions were in good agreement with those calculated by the langmuir mod ... | 2001 | 11315813 |
| isoenzyme multiplicity and characterization of recombinant manganese peroxidases from ceriporiopsis subvermispora and phanerochaete chrysosporium. | we expressed cdnas coding for manganese peroxidases (mnps) from the basidiomycetes ceriporiopsis subvermispora (mnp1) and phanerochaete chrysosporium (h4) under control of the alpha-amylase promoter from aspergillus oryzae in aspergillus nidulans. the recombinant proteins (rmnp1 and rh4) were expressed at similar levels and had molecular masses, both before and after deglycosylation, that were the same as those described for the mnps isolated from the corresponding parental strains. isoelectric ... | 2001 | 11319083 |
| new pulp biobleaching system involving manganese peroxidase immobilized in a silica support with controlled pore sizes. | attempts have been made to use manganese peroxidase (mnp) for chlorine-free pulp biobleaching, but they have not been commercially viable because of the enzyme's low stability. we developed a new pulp biobleaching method involving mesoporous material-immobilized manganese peroxidase from phanerochaete chrysosporium. mnp immobilized in fsm-16, a folded-sheet mesoporous material whose pore size is nearly the same as the diameter of the enzyme, had the highest thermal stability and tolerance to h(2 ... | 2001 | 11319102 |
| the role of glu39 in mnii binding and oxidation by manganese peroxidase from phanerochaete chrysoporium. | manganese peroxidase (mnp) is a heme-containing enzyme produced by white-rot fungi and is part of the extracellular lignin degrading system in these organisms. mnp is unique among mn binding enzymes in its ability to bind and oxidize mn(ii) and efficiently release mn(iii). initial site-directed mutagenesis studies identified the residues e35, e39, and d179 as the mn binding ligands. however, an e39d variant was recently reported to display wild-type mn binding and rate of oxidation, calling into ... | 2001 | 11329293 |
| spectroelectrochemical study of cellobiose dehydrogenase and diaphorase in a thiol-modified gold capillary in the absence of mediators. | a spectroelectrochemical cell was constructed from a gold capillary with 200 microm inner diameter as a working electrode. this allowed spectroelectrochemical study of liquid samples with available volumes less than 5 microl. the optical measurements were accomplished with an optical fibre spectrometer. the optical path of the cell was about 1 cm. to facilitate electrochemistry of biomolecules, the surface of the gold capillary was modified with thiols. the formal potential, e degrees', of the h ... | 2001 | 11339313 |
| [pulmonary adiaspiromycosis: casual finding in a patient who died of yellow fever]. | during an outbreak of yellow fever (rural form of the infection) occurred recently in the state of goiás, brazil, a patient, with clinical manifestations suggestive of the infection, died in the university hospital of brasilia, df, on the fifth day from admission. postmortem examination revealed, microscopically, the characteristic alterations of the infection, and discovered in the lungs and hilar lymph nodes round microrganisms identified as adiaconidia of emmonsia parva var. crescens. | 2001 | 11340501 |
| stability of immobilized 2,4,6-trinitrotoluene metabolites in soil under long-term leaching conditions. | 2,4,6-trinitrotoluene (tnt)-contaminated soil was remediated by an anaerobic/aerobic slurry process. prior to treatment, the soil was spiked with [14c]-tnt. leaching experiments were carried out with the decontaminated soil to determine the degree of binding of the radiolabel under a variety of conditions. to simulate natural degradation processes of soil organic matter, each of three columns was subjected to a different treatment known to enhance biological transformation over a 92-week period. ... | 2001 | 11351427 |
| a new versatile peroxidase from pleurotus. | lignin peroxidase (lip) and manganese peroxidase (mnp) have been investigated in phanerochaete chrysosporium. a third ligninolytic peroxidase has been described in pleurotus and bjerkandera. two of these versatile peroxidases (vps) have been cloned, sequenced and characterized. they have high affinity for mn(2+), hydroquinones and dyes, and also oxidize veratryl alcohol, dimethoxybenzene and lignin dimers. the deduced sequences show higher identity with ph. chrysosporium lip than mnp, but the mo ... | 2001 | 11356138 |
| heterologous expression of athermostable manganese peroxidase from dichomitus squalens in phanerochaete chrysosporium. | dichomitus squalens belongs to a group of white-rot fungi which express manganese peroxidase (mnp) and laccase but do not express lignin peroxidase (lip). to facilitate structure/function studies of mnp from d. squalens, we heterologously expressed the enzyme in the well-studied basidiomycete, phanerochaete chrysosporium. the glyceraldehyde-3-phosphate-dehydrogenase (gpd) promoter of p. chrysosporium was fused to the coding region of the mnp2 gene of d. squalens, 5 bp upstream of the translation ... | 2001 | 11368016 |
| site-directed mutagenesis of the heme axial ligands in the hemoflavoenzyme cellobiose dehydrogenase. | cellobiose dehydrogenase (cdh) from phanerochaete chrysosporium is an extracellular 90-kda hemoflavoenzyme, organized into an n-terminal heme domain and a c-terminal flavin domain. the amino acid residues met65 and his114 or his163 were suggested to be heme iron ligands. mutations of these residues were made and mutant proteins were characterized. h114a mutant cultures produce a stable hemoflavoenzyme with spectral and kinetic characteristics similar to those of wild-type cdh. the m65a and h163a ... | 2001 | 11396923 |
| engineering of a manganese-binding site in lignin peroxidase isozyme h8 from phanerochaete chrysosporium. | a mn(2+)-binding site was created in the recombinant lignin peroxidase isozyme h8 from phanerochaete chrysosporium. in fungal mn peroxidase, the mn-binding site is composed of glu35, glu39, and asp179. we generated a similar site in lignin peroxidase by generating an anionic binding site. we generated three mutations: asn182asp, asp183lys, and ala36glu. its activity, veratryl alcohol, and mn(2+) oxidation were compared to those of native recombinant enzyme and to fungal mn peroxidase isozyme h4, ... | 2001 | 11396962 |
| the effect of agitation and nitrogen concentration on lignin peroxidase (lip) isoform composition during fermentation of phanerochaete chrysosporium. | convective interaction media (cim) monolithic columns were applied for the hplc monitoring of phanerochaete chrysosporium lignin peroxidase (lip) isoforms during cultivation. the influence of the agitation mode (circular, elliptic) and rate (130 and 200 rpm), as well as the initial nitrogen concentration (1.6-6 mm) in the growth medium was investigated. identical rotation rate but different agitation modes resulted in different lip activities and isoenzyme compositions. on the other hand, at dif ... | 2001 | 11403852 |
| deterioration and spoilage of peanuts and desiccated coconuts from two sub-saharan tropical east african countries due to the associated mycobiota and their degradative enzymes. | a broad variety of fungi (84 species belonging to 36 genera) were identified with more taxa infesting peanut seed samples from two tropical countries (29 genera and 61 species) compared to those found in desiccated coconuts (20 genera and 55 species) on both drbc and dg18 media. this may be due to the higher moisture levels in peanuts (5.07-7.97%) compared with coconuts (1.5-4.17%). more taxa and propagules were recovered on dg18 in both cases. the dominant fungi from both substrates on both iso ... | 2001 | 11407493 |
| laccase production by phanerochaete chrysosporium--an artefact caused by mn(iii)? | the possibility of laccase production by phanerochaete chrysosporium was studied. | 2001 | 11412353 |
| role of disulfide bonds in the stability of recombinant manganese peroxidase. | phanerochaete chrysosporium manganese peroxidase (mnp) [isoenzyme h4] was engineered with additional disulfide bonds to provide structural reinforcement to the proximal and distal calcium-binding sites. this rational protein engineering investigated the effects of multiple disulfide bonds on the stabilization of the enzyme heme environment and oxidase activity. stabilization of the heme environment was monitored by uv-visible spectroscopy based on the electronic state of the alkaline transition ... | 2001 | 11434786 |
| purification and characterization of glutathione conjugate reductase: a component of the tetrachlorohydroquinone reductive dehalogenase system from phanerochaete chrysosporium. | a membrane-bound glutathione s-transferase and a soluble glutathione conjugate reductase constitute the reductive dehalogenase system of p. chrysosporium. this enzyme system reductively removes chlorine substituents from tetrachlorohydroquinone, a metabolite of pentachlorophenol. the membrane-bound glutathione s-transferase converts tetrachlorohydroquinone to s-glutathionyltrichloro-1,4-hydroquinone, which is subsequently reduced to 3,5,6-trichlorohydroquinone by the soluble glutathione conjugat ... | 2001 | 11437359 |
| biodecolorization screening of synthetic dyes by four white-rot fungi in a solid medium: possible role of siderophores. | four selected fungi were screened for their ability to decolourize a textile effluent and commercial reactive dyes in a solid medium. | 2001 | 11442809 |
| combined treatment of textile effluent using the sequence phanerochaete chrysosporium-ozone. | textile effluents cause a high environmental impact when released into the environment without correct treatment. in this work, we have evaluated the capacity of treatment of a textile effluent using a biological and a chemical method using the sequence phanerochaete chrysosporium-ozone. the fungal treatment was performed by direct incubation of a fungus spore suspension in textile effluent for nine days. then, the effluent was ozonized at ph 11 and room temperature. color, total organic carbon, ... | 2001 | 11444311 |
| hair and scalp mycobiota in school children in nablus area. | hair and scalp mycobiota of 1389 clinically normal children aged 6-12 years attending 12 schools in the nablus district, palestinian authority, was assessed on three occasions over 8-month period (october 1998-may 1999) using the hair brush technique. one hundred and one fungal species belonging to 33 genera were recovered: 6 dermatophytes, 16 dermatophyte-like keratinophilic fungi, and 79 other keratinophilic fungal species. species varied considerably in their frequency of occurrence and abund ... | 2001 | 11469760 |
| isolation and characterization of mn(iii) tartrate from phanerochaete chrysosporium culture broth. | high initial mn(ii) concentration results in accumulation of a mn(iii) tartrate complex in the growth medium of phanerochaete chrysosporium. since mn(iii) is the major oxidant in ligninolysis by manganese peroxidase, the role of accumulated complex should not be neglected when degradation experiments by a crude culture filtrate are performed. to study the mn(iii) complex oxidative potential it was isolated by absorption to polyamide followed by desorption with an alkaline methanol solution. high ... | 2001 | 11470372 |
| chrysosporium tropicum efficacy against anopheles stephensi larvae in the laboratory. | the keratinophilic soil fungus chrysosporium tropicum was evaluated as a biological control agent against anopheles stephensi larvae in the laboratory. culture filtrates of c. tropicum were found to be toxic to an. stephensi larvae at various concentrations. the ranges of the 50% lethal concentration (lc50) values of fungal filtrates were 16.60-17.78, 12.02-12.88, and 34.67-35.48 microl/ml against 1st-, 2nd-, and 3rd-stage larvae, respectively. the ranges of lc99 values were 38.90-63.10, 12.02-2 ... | 2001 | 11480820 |
| tmc-69, a new antitumor antibiotic with cdc25a inhibitory activity, produced by chrysosporium sp. tc1068. taxonomy, fermentation and biological activities. | a new antibiotic designated tmc-69 has been isolated from the fermentation broth of a fungal strain chrysosporium sp. tc 1068. tmc-69 exhibited moderate in vitro cytotoxic activity. tmc-69-6h, a derivative of tmc-69 prepared by hydrogenation, possessed more potent in vitro cytotoxicity than tmc-69, and exhibited in vivo antitumor activity against murine p388 leukemia and b16 melanoma. tmc-69-6h was found to specifically inhibit cdc25a and b phosphatases. | 2001 | 11480885 |
| oxidation of triphenylarsine to triphenylarsineoxide by trichoderma harzianum and other fungi. | arsenic resistant strains of bacteria and fungi were isolated from soil contaminated by chemical warfare agents. until now, no metabolic products of microbial attack against the phenyl residues of the model substrate triphenylarsine (tp) were found if it was incubated together with these strains in liquid culture assays. however, one of the isolated fungi, trichoderma harzianum as 11, was found to oxidize tp to triphenylarsineoxide (tpo). the yeast trichosporon mucoides sbug 801 and the white-ro ... | 2001 | 11482658 |
| biodegradation of bioaccessible textile azo dyes by phanerochaete chrysosporium. | azo dyes are important chemical pollutants of industrial origin. textile azo dyes with bioaccessible groups for lignin degrading fungi, such as 2-methoxyphenol (guaiacol) and 2,6-dimethoxyphenol (syringol), were synthesised using different aminobenzoic and aminosulphonic acids as diazo components. the inocula of the best biodegradation assays were obtained from a pre-growth medium (pam), containing one of the synthesised dyes. the results of the dye biodegradation assays were evaluated every 7 d ... | 2001 | 11500201 |
| a packed-bed fungal bioreactor for the continuous decolourisation of azo-dyes (orange ii). | the degradation of an azo dye, orange ii, by immobilised phanerochaete chrysosporium in a continuous packed bed bioreactor for periods longer than 30 days has been carried out. nearly complete decolourisation (>95%) was achieved when working at a high dye load rate of 0.2 g x l(-1) x d(-1), a temperature of 37 degrees c, a hydraulic retention time (hrt) of 24 h and applying oxygen gas in a pulsed flow. these conditions allowed manganese peroxidase (mnp) production and the subsequently orange ii ... | 2001 | 11500202 |
| decolorization of textile indigo dye by ligninolytic fungi. | the indigo dye is extensively used by textile industries and is considered a recalcitrant substance, which causes environmental concern. chemical products used on textile processing, which affect the environment through effluents, can be voluminous, colored and varied. vat textile dyes, like indigo, are often used and dye mainly cellulosic fibers of cotton. decolorization of this dye in liquid medium was tested with ligninolytic basidiomycete fungi from brazil. decolorization started in a few ho ... | 2001 | 11500207 |
| application of microassays for investigation of cellulase abrasive activity and backstaining. | model microassays were used for testing the denim-washing performance and indigo backstaining for trichoderma reesei and chrysosporium lucknowense commercial cellulase preparations on a 'test-tube scale'. c. lucknowense preparation demonstrated a higher potential in the denim biostoning process. the performance of four purified cellulases (two endoglucanases and two cellobiohydrolases) from c. lucknowense on cotton textiles was assayed, and the key enzyme (endoglucanase with a molecular weight o ... | 2001 | 11500217 |
| biological degradation of 2,4,6-trinitrotoluene. | nitroaromatic compounds are xenobiotics that have found multiple applications in the synthesis of foams, pharmaceuticals, pesticides, and explosives. these compounds are toxic and recalcitrant and are degraded relatively slowly in the environment by microorganisms. 2,4,6-trinitrotoluene (tnt) is the most widely used nitroaromatic compound. certain strains of pseudomonas and fungi can use tnt as a nitrogen source through the removal of nitrogen as nitrite from tnt under aerobic conditions and the ... | 2001 | 11527999 |
| an amperometric cellobiose dehydrogenase-based biosensor can be used for measurement of cellulase activity. | the hemoflavoenzyme cellobiose dehydrogenase (cdh, ec 1.1.99.18) from phanerochaete chrysosporium has been used in an amperometric redox polymer-based biosensor. used in conjugation with a fia system this biosensor can replace colorimetric assays for measuring cellobiose liberated from cellulose in a series of cellulase-containing samples. the biosensor gave the same result as the somogyi-nelson method in a less time-consuming and laborious manner. the two methods showed about the same precision ... | 2001 | 11237326 |
| inhibition of lignin peroxidase-mediated oxidation activity by ethylenediamine tetraacetic acid and n-n-n'-n'-tetramethylenediamine. | the mineralization rate of lc-[1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane] (ddt) was reduced by 90% on the 18th day in fungal cultures of phanerochaete chrysosporium in the presence of 8 mm ethylenediamine tetraacetic acid (edta). in the presence of 8 mm n-n-n'-n'-tetramethylenediamine (temed), the mineralization rate of 14c-ddt was reduced by 80%. in the presence of 2 mm or 10 mm edta, 95% inhibition of lignin peroxidase (lip) mediated veratryl alcohol oxidase activity and 97% inhibition of ... | 2001 | 11254169 |
| determination of the toxicity of several aromatic carbonylic compounds and their reduced derivatives on phanerochaete chrysosporium using a pseudomonas putida test system. | we tested four aromatic carbonylic compounds and their corresponding reduced derivatives, possible substrates, and products of a biotransformation for toxicity against the white-rot fungus phanerochaete chrysosporium. the bacterium pseudomonas putida, which has been proven to be a good test organism for investigating toxic effects, was used as a primary screen. for both p. chrysosporium and p. putida, all ketones showed a higher toxicity than their corresponding alcohol derivatives. within one c ... | 2001 | 11255153 |
| decolourisation of synthetic and spentwash melanoidins using the white-rot fungus phanerochaete chrysosporium jag-40. | phanerochaete chrysosporium jag-40 was isolated from the soil samples saturated with spilled molasses collected from a sugar mill. this isolate decolourised synthetic and natural melanoidins present in spentwash in liquid fermentation; up to 80% in 6 days at 30 degrees c under aerobic conditions. a large inoculum size stimulated fungal biomass production, but this gave less decolourisation of pigment; 5% w/v (dry weight) mycelial suspension was found optimum for maximum decolourisation in melano ... | 2001 | 11265794 |
| study of protein adsorption on indigo particles confirms the existence of enzyme--indigo interaction sites in cellulase molecules. | adsorption of several crude and purified cellulases (from trichoderma reesei, penicillium verruculosum and chrysosporium lucknowense) on indigo particles and avicel cellulose was studied. much higher amounts of protein were bound to indigo than to cellulose under similar conditions. for different purified enzymes, the quantity of bound protein per mg of adsorbent (indigo or cellulose) varied in the range of 57-111 and 0-62 microg x mg(-1), respectively. however, in general, the enzyme adsorption ... | 2001 | 11267701 |
| transformation of 2,4,6-trinitrotoluene (tnt) by immobilized phanerochaete chrysosporium under fed-batch and continuous tnt feeding conditions. | the cometabolic transformation of 2,4,6-trinitrotoluene (tnt) by an immobilized phanerochaete chrysosporium culture was investigated under different tnt and/or glycerol feeding conditions in a 5-l reactor. in the fed-batch feeding mode, as a result of four spiking events at an average feeding rate of 20 mg tnt l(-1) d(-1) and 250 mg glycerol l(-1) d(-1), the initial tnt transformation rate and the glycerol uptake rate of the 7-day-old immobilized cell culture were 2.41 mg l(-1) h(-1) and 16.6 mg ... | 2001 | 11283910 |
| model coupling intraparticle diffusion/sorption, nonlinear sorption, and biodegradation processes. | diffusion, sorption and biodegradation are key processes impacting the efficiency of natural attenuation. while each process has been studied individually, limited information exists on the kinetic coupling of these processes. in this paper, a model is presented that couples nonlinear and nonequilibrium sorption (intraparticle diffusion) with biodegradation kinetics. initially, these processes are studied independently (i.e., intraparticle diffusion, nonlinear sorption and biodegradation), with ... | 2001 | 11291476 |
| pcb congener selective biodegradation by the white rot fungus pleurotus ostreatus in contaminated soil. | six strains of white rot fungi were tested for their biodegradation ability of low chlorinated polychlorinated biphenyl (pcb) commercial mixture (delor 103) in real soil system. phanerochaete chrysosporium and trametes versicolor did not show any ability to degrade pcbs in soil. on the contrary, four strains of pleurotus ostreatus were able to remove about 40% of delor 103 in two months. all p. ostreatus strains decomposed pcbs selectively with the preference for congeners with chlorine atoms in ... | 2001 | 11297400 |
| pneumocystosis in wild small mammals from california. | cyst forms of the opportunistic fungal parasite pneumocystis carinii were found in the lungs of 34% of the desert shrew, notiosorex crawfordi (n = 59), 13% of the ornate shrew, sorex ornatus (n = 55), 6% of the dusky-footed wood rat, neotoma fuscipes (n = 16), 2.5% of the california meadow vole, microtus californicus (n = 40), and 50% of the california pocket mouse, chaetodipus californicus (n = 2) caught from southern california between february 1998 and february 2000. cysts were not found in a ... | 2001 | 11310900 |
| the green fluorescent protein gene functions as a reporter of gene expression in phanerochaete chrysosporium. | the enhanced green fluorescent protein (gfp) gene (egfp) was used as a reporter of gene expression driven by the glyceraldehyde-p-dehydrogenase (gpd) gene promoter and the manganese peroxidase isozyme 1 (mnp1) gene promoter in phanerochaete chrysosporium. four different constructs were prepared. puggm3' and pugigm3' contain the p. chrysosporium gpd promoter fused upstream of the egfp coding region, and pumgm3' and pumigm3' contain the p. chrysosporium mnp1 promoter fused upstream of the egfp gen ... | 2001 | 11157267 |
| crystal structures of pristine and oxidatively processed lignin peroxidase expressed in escherichia coli and of the w171f variant that eliminates the redox active tryptophan 171. implications for the reaction mechanism. | the heme enzyme lignin peroxidase (lip) from the white rot fungus phanerochaete chrysosporium contains a solvent exposed redox active tryptophan residue (trp171) that carries a unique hydroxy group stereo-specifically attached to its c(beta) atom. a trp171phe mutant has no activity at all towards the substrate veratryl alcohol. the mechanism of veratryl alcohol oxidation involving beta-hydroxy-trp171 is largely unknown. here, we present the first crystal structures of lip isozyme h8 at high reso ... | 2001 | 11162097 |
| biodegradation of polycyclic aromatic hydrocarbons (pahs) by native microflora and combinations of white-rot fungi in a coal-tar contaminated soil. | four white-rot fungi (phanerochaete chrysosporium imi 232175, pleurotus ostreatus from the university of alberta microfungus collection imi 341687, coriolus versicolor imi 210866 and wye isolate #7) and all possible combinations of two or more of these fungi, were incubated in microcosms containing wheat straw and non-sterile coal-tar contaminated soil to determine their potential to degrade polycyclic aromatic hydrocarbons (pahs). biotic and abiotic controls were prepared similarly and pah conc ... | 2001 | 11131793 |
| catalase activities of phanerochaete chrysosporium are not coordinately produced with ligninolytic metabolism: catalases from a white-rot fungus. | phanerochaete chrysosporium uses hydrogen peroxide as a substrate in its ligninolytic phase. to determine how the fungus protects itself against detrimental effects of reactive oxygen species, catalase activity was examined. the fungus produced up to four different catalase isozymes, cat a to d. isozyme catc was the dominant activity in all growth conditions. a minor catalase, catd, was apparent in low-carbon culture and upon exposure of mycelium grown on high-nitrogen medium with 5-50 mm of hyd ... | 2001 | 11116389 |
| pcb biodegradation in aged contaminated soil: interactions between exogenous phanerochaete chrysosporium and indigenous microorganisms. | this work investigated whether the interaction between the white-rot fungus phanerochaete chrysosporium and indigenous microorganisms could enhance polychlorinated biphenyl (pcb) removal from historically contaminated soil in aerobic microcosms. the pcb mixture was composed mainly of 14% tri-, 20% tetra-, 9% penta-, 17% hexa-, 26% hepta-, 11% octa-, and 3% nona-chlorobiphenyl (cb) congeners, determined by gc/ms. the fungus, which was grown on sugarcane bagasse and added via this solid substrate, ... | 2001 | 11545344 |
| treatment of wastewater sludge by liquid state bioconversion process. | this study was conducted to evaluate the effect of an eminent decay fungus, phanerocheate chrysosporium of organic residues on wastewater sludge for its improvement through decomposition and separation of waste particles by liquid state bioconversion (lsb). the effect of fungal treatment was compared to uninoculated (control) at three different harvests 7, 14 and 21 days after inoculation (dai). the observed results showed that the weight loss and solid content of wastewater sludge were signific ... | 2001 | 11545349 |
| fungicidal activity of beta-thujaplicin analogues. | the fungicidal activity of analogues of beta-thujaplicin, a natural product responsible for the durability of heartwood of several cupressaceous trees, was investigated in vitro on the growth of different white and brown rot fungi involved in wood biodegradation, coriolus versicolor, phanerochaete chrysosporium, poria placenta and gloephyllum trabeum. the study shows that 2-hydroxycyclohepta-2,4,6-trienone (tropolone), easily prepared according to a literature procedure, possesses interesting fu ... | 2001 | 11561410 |
| solid-state production of lignin peroxidase (lip) and manganese peroxidase (mnp) by phanerochaete chrysosporium using steam-exploded straw as substrate. | in the used media mainly consisting of steam-exploded wheat straw, the straw, which could replace expensive veratryl alcohol, might act not only as nutrient, but also as inducer of lignin enzymes. the activities of the enzymes lignin peroxidase (lip) and manganese peroxidase (mnp) in solid-state fermentation (ssf) were far higher than in submerged fermentation (smf). under optimal conditions of ssf, the maximum activities of the enzymes lip and mnp were 2600 and 1375 u/l, respectively. thus, thi ... | 2001 | 11563706 |
| production and characterization of recombinant phanerochaete chrysosporium cellobiose dehydrogenase in the methylotrophic yeast pichia pastoris. | the hemoflavoenzyme cellobiose dehydrogenase (cdh) from the white-rot fungus phanerochaete chrysosporium has been heterologously expressed in the methylotrophic yeast pichia pastoris. after 4 days of cultivation in the induction medium, the expression level reached 1800 u/l (79 mg/l) of cdh activity, which is considerably higher than that obtained previously for wild-type cdh (wtcdh) and recombinant cdh (rcdh) produced by p. chrysosporium. analysis with sds-page and coomassie brilliant blue (cbb ... | 2001 | 11676020 |
| roles of two white-rot basidiomycete fungi in decolorisation and detoxification of olive mill waste water. | a phanerochaete chrysosporium strain was isolated from moroccan olive mill waste water (omw) and its ability to degrade omw in different culture conditions was investigated and compared to that of pleurotus ostreatus. the results indicated that ph. chrysosporium isolate is more efficient than pl. ostreatus in decolorising and detoxifying omw in the presence of added nutrients. ph. chrysosporium is able to remove more than 50% of the colour and phenols from omw within 6 days of incubation, wherea ... | 2001 | 11693925 |
| engineering of the h2o2-binding pocket region of a recombinant manganese peroxidase to be resistant to h2o2. | the manganese peroxidase produced by phanerochaete chrysosporium, which catalyzes the oxidation of mn(2+) to mn(3+), is easily inactivated by the hydrogen peroxide (h2o2) presented in the reaction. we attempted to increase h2o2 resistance by the conformational stabilization around the h2o2-binding pocket. based on its structural model, engineering of oxidizable met273 located near the pocket to a non-oxidizable leu showed a great improvement. furthermore, after treatment at 1 mm h2o2 where the w ... | 2001 | 11734216 |
| family 7 cellobiohydrolases from phanerochaete chrysosporium: crystal structure of the catalytic module of cel7d (cbh58) at 1.32 a resolution and homology models of the isozymes. | cellobiohydrolase 58 (cel7d) is the major cellulase produced by the white-rot fungus phanerochaete chrysosporium, constituting approximately 10 % of the total secreted protein in liquid culture on cellulose. the enzyme is classified into family 7 of the glycosyl hydrolases, together with cellobiohydrolase i (cel7a) and endoglucanase i (cel7b) from trichoderma reesei. like those enzymes, it catalyses cellulose hydrolysis with net retention of the anomeric carbon configuration. the structure of th ... | 2001 | 11743726 |
| rheological behavior of phanerochaete chrysosporium broth during lignin degradation. | rheological behavior of a lignin-degrading culture of phanerochaete chrysosporium cdbb-h-298 was determined. rheological characteristics revealed a newtonian behavior in the culture medium without fungi, and a non-newtonian dilatant behavior with fungi. in addition, the rheological behavior of the lignin-containing culture medium was complex, and changed from dilatant to pseudoplastic. during fungal growth without lignin in kirk media rheological behavior exhibited a parabolic profile, and thixo ... | 2001 | 11759909 |
| human hair colonizing fungi in water sediments of india. | out of 144 samples of water sediments, 183 isolates belonging to 9 genera and 22 species were isolated. fifty-nine isolates of acremonium, 26 of chrysosporium indicum, 22 of chrysosporium keratinophilum, 17 of malbranchea sp. and 10 of microsporum gypseum were recovered. acremonium implicatum, chrysosporium georgii, chrysosporium xerophilum and geomyces pannorum were reported for the first time from india. | 2001 | 11761148 |
| 14-(2 ',3 ',5 '-trihydroxyphenyl)tetradecan-2-ol, a novel acetylcholinesterase inhibitor from chrysosporium sp. | 2001 | 11776443 | |
| studies on the decolourisation of an artificial textile-effluent by white-rot fungi in n-rich and n-limited media. | coriolopsis gallica and phanerochaete chrysosporium were selected for their potential ability to degrade five dyes in an artificial effluent. degradation experiments were carried out in n-rich (c:n ratio 11.6:1) and n-limited (116:1) conditions at an effluent concentration of 100 mg l(-1). p. chrysosporium decolourised 53.6% of the effluent in n-rich conditions and 48% in n-limited conditions. c. gallica decolourised 80.7% in n-rich conditions and 86.9% in n-limited conditions. nitrogen suppleme ... | 2001 | 11778898 |
| [effect of cyclodextrins on fungal degradation of fluorene]. | the purpose of this study was to improve the bioavailability of fluorene (pah) by the use of complexing agents, cyclodextrins. the biodegradation tests were performed in liquid medium batches; fluorene was quantified by hplc. experimental results showed the enhancement of fluorene degradation by penicillium italicum and phanerochaete chrysosporium in the presence of branched cyclodextrins. | 2001 | 11924508 |
| utilisation of lignocellulosic wastes for lignin peroxidase production by semi-solid-state cultures of phanerochaete chrysosporium. | in the present work, the production of ligninolytic enzymes by semi-solid-state cultures of phanerochaete chrysosporium bkm-f-1767 (atcc 24725), employing different lignocellulosic wastes as support, was investigated. the waste materials employed were grape seeds, wheat straw and wood shavings. maximum lignin peroxidase activities of 1,620 +/- 123 u/l,364 +/- 35 u/l and 571 +/- 42 u/l were attained, respectively. nevertheless, low manganese-dependent peroxidase activities were found, being insig ... | 2001 | 11995821 |
| biotransformation of linear alkylbenzene sulfonate (las) by phanerochaete chrysosporium: oxidation of alkyl side-chain. | the white rot fungus phanerochaete chrysosporium, which generally mineralizes substituted aromatics to co2, transformed linear alkylbenzene sulfonate (las) surfactants mainly at their alkyl side chain. degradation of las was evidenced by a zone of clearing on las-containing agar plates and colorimetric analysis of liquid cultures. disappearance of las was virtually complete within 10 days in low nitrogen (2.4 mm n), high nitrogen (24 mm n) and malt extract (me) liquid media. after 5 days of incu ... | 2001 | 12051650 |
| soil saprotrophic microfungi associated with roots of calamagrostis epigeios on an abandoned deposit of toxic waste from smelter factory processing pyrite raw materials. | saprotrophic microfungi associated with roots of calamagrostis epigeios growing in industrial waste contaminated with high concentrations of heavy metals (manganese, iron, zinc) were isolated on three different media. the isolates were obtained from three plots colonized by plant cenoses, representing three different succession series of plant cover: initial, intermediate, and advanced. a total of 60 species of saprotrophic microfungi were found in the roots during three samplings. in addition, ... | 2001 | 12032621 |
| biodegradation of a polymeric dye in a pulsed bed bioreactor by immobilised phanerochaete chrysosporium. | an immobilised fungal bioreactor operated with pulsation of the gas-phase is proposed and operated at variable conditions for the continuous biological degradation of complex structures. in order to test the system, a hardly biodegradable dye (poly r-478) was selected as a model compound and phanerochaete chrysosporium as the ligninolytic fungus. high percentages of decolourisation--between 65% and 80%--under optimal conditions were achieved. moreover, the system proved to have high stability wi ... | 2002 | 12044089 |
| removal of surfactant solubilized polycyclic aromatic hydrocarbons by phanerochaete chrysosporium in a rotating biological contactor reactor. | white rot fungi can oxidize surfactant solubilized polycyclic aromatic hydrocarbons (pah). the objective of this study was to evaluate the performance of immobilized white rot fungus, phanerochaete chrysosporium, to remove surfactant tween 80 solubilized pah i.e. phenanthrene, pyrene and benzo(alpha)pyrene in a rotating biological contactor (rbc) reactor. results indicated that the immobilized p. chrysosporium in the rbc reactor system in continuous operation could effectively remove the three t ... | 2002 | 12044552 |
| did earthworms contribute to the parasitic evolution of dermatophytes? | the survival of dermatophyte species in the gut of four species of earthworms was studied by feeding the fungi to the earthworms. recovery of the dermatophyte species in culture from the guts was only possible for microsporum gypseum and chrysosporium keratinophilum. in the light of these findings, we presume that earthworms could have influenced the parasitic evolution of certain dermatophytes. | 2002 | 12421289 |
| production of manganese-dependent peroxidase in a new solid-state bioreactor by phanerochaete chrysosporium grown on wood shavings. application to the decolorization of synthetic dyes. | the production of manganese-dependent peroxidase (mnp) by phanerochaete chrysosporium in a new solid-state bioreactor, the immersion bioreactor, operating with lignocellulosic waste, such as wood shavings, was investigated. maximum mnp and lignin peroxidase (lip) activity of 13.4 and 8.48 mukat/l were obtained, respectively. the in vitro decolorization of several synthetic dyes by the extracellular liquid produced in the above-mentioned bioreactor (containing mainly mnp) was carried out and its ... | 2002 | 12422521 |
| immunohistochemical characterization of tuberculous and non-tuberculous lesionsin naturally infected european badgers (meles meles). | a panel of species cross-reactive antibodies was established for the immunohistochemical labelling of phagocytic and lymphoid cells in formalin-fixed normal badger tissues. these reagents were used to investigate the immunopathogenesis of both tuberculous and non-tuberculous granulomas in badgers. in normal badger tissues, antisera specific for the cd79a and cd79b epitopes strongly labelled follicular b lymphocytes and plasma cells in lymph nodes, bronchus-associated lymphoid tissue and peyer's ... | 2002 | 12056773 |
| causative agents of nosocomial mycoses. | in the last few years mycoses have been caused by fungi formerly considered to be harmless for humans. they cause diseases of plants and insects; some of them are also used in the industry. they are now usually called "emerging fungi". we investigated this flora with respect to their potential to cause infections in hospitals. these fungi are present in the air, on medical objects and instrumentation, in the respiratory tract and on the hands of hospital staff; other sources have been identified ... | 2002 | 12058386 |
| chrysosporium anamorph of nannizziopsis vriesii associated with fatal cutaneous mycoses in the salt-water crocodile (crocodylus porosus). | the chrysosporium anamorph of nannizziopsis vriesii, recently identified as the cause of cutaneous infections in chameleons and brown tree snakes, was associated with skin infections and deaths in salt-water crocodile (crocodylus porosus) hatchlings on two separate occasions 3 years apart. in all, 48 animals died from the infection. all hatchlings came from the same farm in northern queensland, australia. | 2002 | 12058727 |
| decolorization of synthetic textile dyes by lignin peroxidase of phanerochaete chrysosporium. | neem hull waste (containing a high amount of lignin and other phenolic compounds) was used for lignin peroxidase production by phanerochaete chrysosporum under solid-state fermentation conditions. maximum decolorization achieved by partially purified lignin peroxidase was 80% for porocion brilliant blue hgr, 83 for ranocid fast blue, 70 for acid red 119 and 61 for navidol fast black msrl. the effects of different concentrations of veratryl alcohol, hydrogen peroxide, enzyme and dye on the effici ... | 2002 | 12094739 |
| oxygen reduction by cellobiose oxidoreductase: the role of the haem group. | we have used optical and electron paramagnetic spectroscopy to study the flavohaem enzyme cellobiose oxidoreductase (cbor) from phanerochaete chrysosporium. we have examined redox cycles of the enzyme in which the oxidation of cellobiose to cellobionolactone is coupled to the reduction of oxygen. during turnover flavin can reduce oxygen with one electron to produce superoxide or two electrons to produce hydrogen peroxide. addition of superoxide dismutase significantly extended the time courses o ... | 2002 | 11997012 |
| occurrence of keratinophilic fungi and dermatophytes on domestic birds in nigeria. | feathers, nails and beaks of one hundred and twenty common birds in nigeria, chicken [50], ducks [20], turkeys [15] and pigeons [35], were examined using the soil plate technique for their mycoflora. 15 species of fungi were recovered and they belong to the genera chrysosporium, trichophyton, microsporum, aspergillus, fusarium, mucor rhizopus, penicillium and trichoderma. microsporum gypseum was the species most frequently isolated (35% of the samples). the most common genus was chrysosporium an ... | 2002 | 12000131 |
| bio-degradation of lignin in olive pomace by freshly-isolated species of basidiomycete. | the solid waste (pomace) from olive oil processing cannot be used directly as an animal feed, but it was thought that an appropriate series of fermentations might improve its nutritional value. as a first step, typical samples of pomace were subjected, after an alkaline pre-treatment, to delignification by phanerochaete chrysosporium (atcc 19343), oxysporus sp., schizophyllum commune, hyphoderma sp. or ganoderma sp.; the last four species being freshly isolated from decaying wood collected in a ... | 2002 | 12003313 |
| use of steam explosion liquor from sugar cane bagasse for lignin peroxidase production by phanerochaete chrysosporium. | the possibility of using two by-products of the sugar cane industry, molasses and bagasse steam explosion liquor (sel), for lignin peroxidase (lip) production by phanerochaete chrysosporium was investigated. for comparison, the fungus was initially cultivated in synthetic media containing either glucose, sucrose, xylose, or xylan as sole carbon sources. the effect of veratryl alcohol (va) was also investigated in relation to the enzyme activity levels. results showed that sucrose was not metabol ... | 2002 | 12018256 |
| cellobiose quinone oxidoreductase from the white rot fungus phanerochaete chrysosporium is produced by intracellular proteolysis of cellobiose dehydrogenase. | the fungus phanerochaete chrysosporium was grown in a 10-l automatic fermenter using cellobiose as carbon source to monitor the induction of cellobiose dehydrogenase (cdh) and cellobiose quinone oxidoreductase (cbq) enzymes, and to search for tentative cbq and cdh genes and their transcriptional products. after 24 h of induction, cdh was detected in the culture supernatant and a protein was recognized by a specific anti-cdh polyclonal antibody in the sonicated biomass. northern blot experiments ... | 2002 | 12031479 |
| effect of pollutants on the ergosterol content as indicator of fungal biomass. | ergosterol content was determined in 20 white-rot fungi isolates and the values ranged from 2380 to 13060 microg g(-1) fungal biomass. significant changes of ergosterol content according the physiological stage for bjerkandera adusta 4312 and coriolopsis gallica 8260 were found, showing the highest values during the stationary phase. however, in the case of phanerochaete chrysosporium 3642, no changes were detected during growth. the effect of pollutants, such as heavy metals and fungicides, on ... | 2002 | 12031573 |
| investigation of the response of wood-rotting fungi to copper stress by size-exclusion chromatography and capillary zone electrophoresis with icp ms detection. | a method based on the coupling of size-exclusion chromatography (sec) with inductively coupled plasma mass spectrometry (icp ms) was developed for screening the changes in the bioligand composition of wood-rotting fungi as a function of their exposure to copper stress. strains of four different species of wood-rotting fungi: phanerochaete chrysosporium, schizophyllum commune, daedalea quercina and pleurotus ostreatus were examined. only one, namely ph. chrysosporium, showed any significant diffe ... | 2002 | 11939533 |
| kinetics of inter-domain electron transfer in flavocytochrome cellobiose dehydrogenase from the white-rot fungus phanerochaete chrysosporium. | the pre-steady-state kinetics of inter-domain electron transfer in the extracellular flavocytochrome cellobiose dehydrogenase from phanerochaete chrysosporium was studied using various values of ph and substrate concentration. monitoring at the isosbestic point of each prosthetic group indicated that the reductive half-reactions of flavin and haem were biphasic and monophasic respectively. when the observed rates of the flavin and haem reactions were plotted against substrate concentration, the ... | 2002 | 11939907 |
| responses of coptotermes formosanus and reticulitermes flavipes (isoptera: rhinotermitidae) to three types of wood rot fungi cultured on different substrates. | this study examined the responses of two termite species, the formosan subterranean termite, coptotermes formosanus shiraki, and the eastern subterranean termite, reticulitermes flavipes (kollar), to three types of wood decay fungi: a brown rot fungus, gloeophyllum trabeum (persoon: fries) murrill; a white rot fungus, phanerochaete chrysosporium burdsall; and a litter rot fungus, marasmiellus troyanus (murrill) singer. we also examined the responses of termites to these three types of fungi grow ... | 2002 | 11942746 |
| biodegradation of volatile organic compounds by five fungal species. | five fungal species, cladosporium resinae (atcc 34066), cladosporium sphaerospermum (atcc 200384), exophiala lecanii-corni (cbs 102400), mucor rouxii (atcc 44260), and phanerochaete chrysosporium (atcc 24725), were tested for their ability to degrade nine compounds commonly found in industrial off-gas emissions. fungal cultures inoculated on ceramic support media were provided with volatile organic compounds (vocs) via the vapor phase as their sole carbon and energy sources. compounds tested inc ... | 2002 | 11956756 |
| reactions of the class ii peroxidases, lignin peroxidase and arthromyces ramosus peroxidase, with hydrogen peroxide. catalase-like activity, compound iii formation, and enzyme inactivation. | the reactions of the fungal enzymes arthromyces ramosus peroxidase (arp) and phanerochaete chrysosporium lignin peroxidase (lip) with hydrogen peroxide (h(2)o(2)) have been studied. both enzymes exhibited catalase activity with hyperbolic h(2)o(2) concentration dependence (k(m) approximately 8-10 mm, k(cat) approximately 1-3 s(-1)). the catalase and peroxidase activities of lip were inhibited within 10 min and those of arp in 1 h. the inactivation constants were calculated using two independent ... | 2002 | 11983689 |
| biodecolourization of azo and triphenylmethane dyes by dichomitus squalens and phlebia spp. | nine white-rot fungal strains were screened for biodecolourization of brilliant green, cresol red, crystal violet, congo red and orange ii. dichomitus squalens, phlebia fascicularia and p. floridensis decolourized all of the dyes on solid agar medium and possessed better decolourization ability than phanerochaete chrysosporium when tested in nitrogen-limited broth medium. | 2002 | 11986919 |
| optimization of an extracellular protease of chrysosporium keratinophilum and its potential in bioremediation of keratinic wastes. | chrysosporium keratinophilum imi 338142 isolated from a waste site containing organopollutants was studied for its ability to produce extracellular proteases on glucose-gelatin medium. fungus was observed to be a potent producer of such enzymes. enzyme secretion was best at 15 days of incubation period at ph 8 and temperature 40 degrees c. asparagine was repressive to protease expression. no relationship existed between the enzyme yield and increase in biomass. exogenous sugars suppressed enzyme ... | 2002 | 12749577 |
| morphological characterization of in-vitro human hair keratinolysis, produced by identified wild strains of chrysosporium species. | chrysosporium species were isolated from soil and keratinized material. primary isolation was performed following the general method of hair baiting on modified czapek-agar media with washed, defated and sterilized human hair fragments added. strains were maintained in test tubes of potato dextrose agar at 29 degrees c and cultivated on phytone yeast extract agar at 28 degrees c for 14 days for identification. isolates were characterized using van oorschot's key. keratinolytic activity was expre ... | 2002 | 12749579 |
| incidence of keratinophilic fungi from selected soils of kerala state (india). | one hundred and fifty-eight soil samples were collected from various areas of four districts of kerala and screened for prevalence of keratinophilic fungi and related dermatophytes. from the positive samples (60.75%), a total of eight genera with 15 species were isolated viz., arthroderma simii (0.63%), chrysosporium indicum (20.25%), c. keratinophilum (6.96%). c. lobatum (1.26%), c. pannicola (1.26%), c. tropicum (5.06%), chrysosporium state of arthroderma cuniculi (1.26%), chrysosporium state ... | 2002 | 12749581 |
| ecology of dermatophytes and other keratinophilic fungi in swimming pools and polluted and unpolluted streams. | the biodiversity and richness of keratinophilic fungal communities including dermatophytes were assessed in three stream sites and three swimming pools in the nablus district in palestine, using hair baiting (hbt) and surface dilution plate (sdp) techniques, over 8- and 6-month periods, respectively. the effect of wastewater effluent and selected ecological factors on these fungi in relation to species diversity and population densities were also considered. fifty keratinophilic fungal species w ... | 2002 | 12749584 |
| [bioremediation for petroleum-contaminated soil by composting technology]. | with composting technology of off-site bioremediation, the bioremediation of soil contaminated by crude oil from liaohe oil field was studied. 4 treatments units were set, each units being 118.5 cm in length, 65.5 cm in width, and 12.5 cm in height. the results showed that when the soil was contaminated with 5.22 g.100 g-1 tph, the degradation rate of tph reached 54.2% after 55 days operation by phanerochaete chrysosporium. the results also indicated that the major factors that effected the tph ... | 2002 | 12561178 |
| characterization of manganese-dependent peroxidase isoenzymes from the ligninolytic fungus phanerochaete flavido-alba. | phanerochaete flavido-alba is able to decolorize and detoxify olive oil wastewater (omw) in a process in which simple and polymeric phenols are removed. an unusual acidic mnp is accumulated during the degradation course. this microorganism produces two families of mnps. mnp1 has an apparent molecular weight of 45 kda and is secreted as a mixture of isoenzymes with pi ranging from 5.6 to 4.75. mnp2, which is produced as an unique isoenzyme, has an apparent molecular weight of 55.6 mr and an unusu ... | 2002 | 12437216 |
| phanerochaete chrysosporium nadph-cytochrome p450 reductase kinetic mechanism. | the recently completed genome of the basidiomycete, phanerochaete chrysosporium, revealed the presence of one nadph-cytochrome p450 oxidoreductase (cpr; ec 1.6.2.4) gene and >123 cytochrome p450 (cyp) genes. how a single cpr can drive many cyps is an important area of study. we have investigated this cpr to gain insight into the mechanistic and structural biodiversity of the cytochrome p450 catalytic system. native cpr and a nh(2)-terminally truncated derivative lacking 23 amino acids have been ... | 2002 | 12437968 |
| disseminated pulmonary adiaspiromycosis caused by emmonsia crescens in a horse. | 2002 | 12462193 | |
| polycyclic aromatic hydrocarbon removal from soil by surfactant solubilization and phanerochaete chrysosporium oxidation. | surfactant soil washing can remove polycyclic aromatic hydrocarbons (pahs) from contaminated soil, and the white rot fungus, phanerochaete chrysosporium burdsall in burdsall & eslyn, can oxidize pahs. the objective of this study was to develop a novel bioremediation technology using a combination of abiological surfactant soil washing followed by pah biological oxidation in soil washwater using p. chrysosporium in a rotating biological contactor (rbc) reactor. soil used for experimentation was a ... | 2002 | 12469833 |
| isolation of keratinophilic fungi from elementary schools and public parks in isfahan, iran. | a total of 330 dust and soil samples collected from different sites at 13 elementary schools and seven public parks in the province of isfahan, iran were examined for the frequency of keratinophilic fungi by the hair-baiting technique. two hundred and fourteen isolates of keratinophilic fungi belonging to seven species were identified. the most frequent isolate was chrysosporium keratinophilum (54.2%). the frequency and distribution of these keratinophilic fungi are discussed in relation to the ... | 2002 | 12472728 |
| isolation and structure elucidation of two new antibacterial compounds produced by chrysosporium queenslandicum. | 2002 | 12523825 | |
| non-sporulating chrysosporium: an opportunistic fungal infection in a neutropenic patient. | we report a case of an invasive infection with non-sporulating chrysosporium species in a patient who was treated with chemotherapy for relapsed acute lymphoblastic leukemia. this patient presented with a persistent lobar pneumonia, skin lesions, and possible involvement of the central nervous system. the patient responded to treatment with amphotericin b and oral itraconazole. | 2002 | 14569730 |
| crystal structure of the flavoprotein domain of the extracellular flavocytochrome cellobiose dehydrogenase. | cellobiose dehydrogenase (cdh) participates in the degradation of cellulose and lignin. the protein is an extracellular flavocytochrome with a b-type cytochrome domain (cyt(cdh)) connected to a flavodehydrogenase domain (dh(cdh)). dh(cdh) catalyses a two-electron oxidation at the anomeric c1 position of cellobiose to yield cellobiono-1,5-lactone, and the electrons are subsequently transferred from dh(cdh) to an acceptor, either directly or via cyt(cdh). here, we describe the crystal structure of ... | 2002 | 11786022 |
| solid-state production of biopulp by phanerochaete chrysosporium using steam-exploded wheat straw as substrate. | a novel material for biopulp-making, steam-exploded wheat straw (sews), was studied. during the steam explosion process, the hemicellulose was partly degraded and became water-soluble sugar as the carbon resource of the chosen microbe growth, and compared with non-sews, the degradation amount of cellulose decreased and the degradation amount of lignin increased for the fermented steam-exploded wheat straw (fsews) cultured with phanerochaete chrysosporium me-446. under the optimum conditions of s ... | 2002 | 11800492 |
| calnexin overexpression increases manganese peroxidase production in aspergillus niger. | heme-containing peroxidases from white rot basidiomycetes, in contrast to most proteins of fungal origin, are poorly produced in industrial filamentous fungal strains. factors limiting peroxidase production are believed to operate at the posttranslational level. in particular, insufficient availability of the prosthetic group which is required for peroxidase biosynthesis has been proposed to be an important bottleneck. in this work, we analyzed the role of two components of the secretion pathway ... | 2002 | 11823227 |
| biodegradation of polyvinyl alcohol by phanerochaete chrysosporium after pretreatment with fenton's reagent. | polyvinyl alcohol (pva) solutions (bp05 and bf17; 5.0%, wt v(-1)) were degraded by a combination of chemical (fenton's reagent) and fungal (phanerochaete chrysosporium) treatments. the overall degradations of bp05 and bf17 were 74.4 and 72.8%, respectively, as determined by chemical oxygen demand (cod) analysis, and 63.7% and 57.7%, respectively, as determined by total organic carbon (toc) analysis. increased retention times and changes in the intensity of the pva peaks on gel permeation chromat ... | 2002 | 11846267 |
| iron-regulated proteins in phanerochaete chrysosporium and lentinula edodes: differential analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis and two-dimensional polyacrylamide gel electrophoresis profiles. | sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and two-dimensional gel electrophoresis (2-de) were used to identify iron-responsive proteins in the white-rot species (phanerochaete chrysosporium and lentinula edodes), by comparing the differential patterns of cellular and membrane proteins obtained from iron-sufficient and iron-deficient mycelia. six cellular proteins induced by iron restriction have been observed in sds-page for p. chrysosporium and twelve for l. edodes. i ... | 2002 | 11870778 |
| lignin peroxidase oxidation of veratryl alcohol: effects of the mutants h82a, q222a, w171a, and f267l. | the site-directed mutations h82a and q222a (residues near the heme access channel), and w171a and f267l (residues near the surface of the protein) were introduced into the gene encoding lignin peroxidase (lip) isozyme h8 from phanerochaete chrysosporium. the variant enzymes were produced by homologous expression in p. chrysosporium, purified to homogeneity, and characterized by kinetic and spectroscopic methods. the molecular masses, the pis, and the uv-vis absorption spectra of the ferric and o ... | 2002 | 11876658 |
| pulmonary colonization by chrysosporium zonatum associated with allergic inflammation in an immunocompetent subject. | we report a case of noninvasive pulmonary disease due to chrysosporium zonatum in an immunocompetent male. the fungus colonized an existing tuberculous cavity and was isolated from transbronchial lavage fluid and from a percutaneous aspiration specimen. the disease was accompanied by the unusual feature of an allergic reaction. the fungus ball was successfully treated by intracavitary administration of amphotericin b. c. zonatum is the anamorph of the heterothallic ascomycete uncinocarpus orissi ... | 2002 | 11880456 |
| biological remediation of explosives and related nitroaromatic compounds. | nitroaromatics form an important group of recalcitrant xenobiotics. only few aromatic compounds, bearing one nitro group as a substituent of the aromatic ring, are produced as secondary metabolites by microorganisms. the majority of nitroaromatic compounds in the biosphere are industrial chemicals such as explosives, dyes, polyurethane foams, herbicides, insecticides and solvents. these compounds are generally recalcitrant to biological treatment and remain in the biosphere, where they constitut ... | 2002 | 11885418 |
| biosorption of lead by phanerochaete chrysosporium in the form of pellets. | the growth of phanerochaete chrysosporium(atcc 24725) in pellets was influenced by culture time, medium ph, c/n, surfactant concentration, spore number in inoculum, and shaking rate. the removal of pb2+ from aqueous solution by this kind of mycelial pellets was studied. the results indicated that many factors affected biosorption. these factors included ph, pb2+ concentration, co-ion, adsorption time, and chemical pretreatments of biomass. under optimum biosorption conditions(ph 4.5, 27 degrees ... | 2002 | 11887305 |
| fungi isolated from skins and pens of healthy animals in nigeria. | the mycoflora of 220 skin scrapings, hair, nail samples and pens' materials of apparently healthy animals including cows, sheep, goats, rabbits, pigs and dogs were determined. twenty eight species of fungi belonging to ten genera were recovered. chrysosporium spp. were the most common and c. keratinophilum was recovered from all animals. dermatophytes which are known causal agents of dermatophytosis were also isolated in different frequencies (microsporum gypseum, m. canis, trichophyton mentagro ... | 2002 | 11913761 |
| 5-demethylovalicin, as a methionine aminopeptidase-2 inhibitor produced by chrysosporium. | 5-demethylovalicin was isolated from the fermentation broth chrysosporium lucknowense and the structure was identified by spectroscopic methods. 5-demethylovalicin inhibited the recombinant human metap-2 (ic(50)=17.7 nm) and the growth of human umbilical vein endothelial cells (huvec; ic(50)=100 nm) in cell proliferation assay without cytotoxicity on the transformed and cancer cell lines. | 2002 | 11738620 |