Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| antibodies from patients with melioidosis recognize burkholderia mallei but not burkholderia thailandensis antigens in the indirect hemagglutination assay. | the indirect hemagglutination assay routinely used to detect antibodies to burkholderia pseudomallei was modified to detect cross-reactivity of antibodies to b. pseudomallei, b. mallei, and b. thailandensis antigens. we demonstrate a lack of cross-reactivity between b. pseudomallei and b. thailandensis but marked cross-reactivity between b. pseudomallei and b. mallei. | 2005 | 16145163 |
| caspase-1 dependent macrophage death induced by burkholderia pseudomallei. | burkholderia pseudomallei is the causative agent for melioidosis, an infectious disease endemic in south-east asia and northern australia. infection can result in a wide spectrum of clinical outcomes, including asymtomatic, acute or chronic conditions. the ability of the bacteria to survive intracellularly within phagocytes and non-phagocytes is postulated to help this pathogen persist in the body during latent chronic conditions. in some gram-negative bacteria, such as shigella and salmonella, ... | 2005 | 16153244 |
| [new diagnostic methods for bacterial infections after the introduction of increased bioterrorism preparedness]. | bacillus anthracis, francicella tularensis, yersinia pestis, burkholderia pseudomallei and brucella melitensis are the bacteria most often mentioned in connection with possible biological terrorist acts. it is known that these microorganisms can be cultured and weaponised for such a purpose. this survey provides examples of the use of diagnostic methods for those microorganisms, further detailed in relation to establishing a national center for biological preparedness in denmark. | 2005 | 16159496 |
| disinfection of burkholderia pseudomallei in potable water. | the effect of chlorine, monochloramine and uv disinfection on the water-borne pathogen burkholderia pseudomallei was assessed. persistence of b. pseudomallei was verified by mpn involving a one-step recovery procedure. chlorine proved the most effective disinfectant with a 99.99% reduction of a 10(6) cfu/ml pure bacterial culture followed by 99.9% reduction by monochloramine and 99% reduction by uv. co-culture of b. pseudomallei with acanthamoeba astronyxis was found to greatly enhance survival ... | 2005 | 15766962 |
| two randomized controlled trials of ceftazidime alone versus ceftazidime in combination with trimethoprim-sulfamethoxazole for the treatment of severe melioidosis. | two antibiotic regimens are used commonly in thailand for the initial treatment of severe melioidosis: ceftazidime in combination with trimethoprim-sulfamethoxazole (tmp-smx) and ceftazidime monotherapy. it is not known whether tmp-smx provides an additional benefit. | 2005 | 16163628 |
| rapid immunofluorescence microscopy for diagnosis of melioidosis. | an immunofluorescent (if) method that detects burkholderia pseudomallei in clinical specimens within 10 min was devised. the results of this rapid method and those of an existing if method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. the sensitivities of both if tests were 66%, and the specificities were 99.5 and 99.4%, respectively. | 2005 | 15817767 |
| a case of melioidosis presenting as migrating pulmonary infiltration: the first case in korea. | melioidosis is an infection of the gram-negative bacterium burkholderia pseudomallei. while it is known as an important cause of sepsis or chronic abscess-forming disease in southeast asia and northern australia, no case has yet been reported in korea. a 50-yr-old man visited our hospital for intermittent fever associated with dry cough and sputum. roentgenographic examination showed migrating pulmonary infiltration. symptoms and chest radiograph and computed tomography (ct) image findings did n ... | 2005 | 15716619 |
| changing epidemiology of melioidosis? a case of acute pulmonary melioidosis with fatal outcome imported from brazil. | melioidosis is an infectious disease caused by burkholderia pseudomallei. it is endemic in south east asia and tropical regions of northern australia. sporadic cases have been described elsewhere. in this article we present a case of acute pulmonary melioidosis with fatal outcome imported from brazil. the most common pathogen causing severe community-acquired pneumonia in brazil is streptococcus pneumoniae. other possible pathogens include legionella spp., mycoplasma pneumonia, gram-negative rod ... | 2005 | 16181507 |
| the burkholderia pseudomallei rpoe (algu) operon is involved in environmental stress tolerance and biofilm formation. | burkholderia pseudomallei, the causative agent of melioidosis, can be isolated from soil and water. to persist, adapt and survive within and outside their human host, bacteria rely on regulatory mechanisms that allow them to respond rapidly to stressful situations. we have examined the possible role of b. pseudomallei alternative sigma factor sigma(e) (rpoe) in the stress response and found that rpoe and its putative regulators (bpre-rseb-mucd) are transcribed in a single transcriptional unit. i ... | 2005 | 16185818 |
| modified virulence of antibiotic-induced burkholderia pseudomallei filaments. | melioidosis is a life-threatening bacterial infection caused by burkholderia pseudomallei. some antibiotics used to treat melioidosis can induce filamentation in b. pseudomallei. despite studies on the mechanism of virulence of the bacteria, the properties of b. pseudomallei filaments and their impact on virulence have not been investigated before. to understand the characteristics of antibiotic-induced filaments, we performed in vitro assays to compare several aspects of virulence between norma ... | 2005 | 15728895 |
| comparison of ashdown's medium, burkholderia cepacia medium, and burkholderia pseudomallei selective agar for clinical isolation of burkholderia pseudomallei. | ashdown's medium, burkholderia pseudomallei selective agar (bpsa), and a commercial burkholderia cepacia medium were compared for their abilities to grow b. pseudomallei from 155 clinical specimens that proved positive for this organism. the sensitivity of each was equivalent; the selectivity of bpsa was lower than that of ashdown's or b. cepacia medium. | 2005 | 16208018 |
| a molecular switch and electronic circuit modulate catalase activity in catalase-peroxidases. | the catalase reaction of catalase-peroxidases involves catalase-specific features built into a peroxidase core. an arginine, 20 a from the active-site heme, acts as a molecular switch moving between two conformations, one that activates heme oxidation and one that activates oxoferryl heme reduction by h(2)o(2), facilitating the catalatic pathway in a peroxidase. the influence of the arginine is imparted to the heme through its association with or dissociation from a tyrosinate that modulates rea ... | 2005 | 16211084 |
| comparison of gatifloxacin, moxifloxacin and ciprofloxacin for treatment of experimental burkholderia pseudomallei infection. | to compare the efficacy of moxifloxacin, gatifloxacin and ciprofloxacin for the post-exposure prophylaxis and treatment of experimental burkholderia pseudomallei infection. the presence of persistent infection in treated animals and the rate of relapse following dexamethasone treatment were also investigated. | 2005 | 15731198 |
| burkholderia pseudomallei invasion and activation of epithelial cells requires activation of p38 mitogen-activated protein kinase. | burkholderia pseudomallei is a causative agent of melioidosis. this gram-negative bacterium is able to survive inside the macrophages and also able to invade non-phagocytic cells including epithelial cells. interaction of pathogenic bacteria to the host cells is frequently associated with activation of mitogen-activated protein (map) kinases signaling activity. in this study, we demonstrated that b. pseudomallei stimulated p38 map kinase of human alveolar lung epithelial cell line (a549). phosph ... | 2005 | 15748812 |
| time-kill curves as a tool for targeting ceftazidime serum concentration during continuous infusion for treatment of septicaemic melioidosis. | melioidosis is a fatal community-acquired infection endemic in tropical areas. ten isolates of the causative microorganism were subjected to time-kill study using a range of ceftazidime concentrations. this study demonstrated that a ceftazidime concentration of eight times the minimum inhibitory concentration yielded an optimal bactericidal effect and should be the target concentration administered by continuous infusion. | 2005 | 16216470 |
| identification of a bacterial factor required for actin-based motility of burkholderia pseudomallei. | burkholderia pseudomallei is a gram-negative facultative intracellular pathogen that enters and escapes from eukaryotic cells using the power of actin polymerization. we have identified a bacterial protein (bima) that is required for the ability of b. pseudomallei to induce the formation of actin tails. bima contains proline-rich motifs and wh2-like domains and shares limited homology at the c-terminus with the yersinia autosecreted adhesin yada. bima is located at the pole of the bacterial cell ... | 2005 | 15773977 |
| melioidosis, northeastern brazil. | melioidosis was first recognized in northeastern brazil in 2003. confirmation of additional cases from the 2003 cluster in ceará, more recent cases in other districts, environmental isolation of burkholderia pseudomallei, molecular confirmation and typing results, and positive serosurveillance specimens indicate that melioidosis is more widespread in northeastern brazil than previously thought. | 2005 | 16229782 |
| antimicrobial activity of some medicinal plants from india. | the results of a preliminary antimicrobial screening of the methanol extracts of zingiber officinale, asteracantha longifolia, citrus acida, salacia microsperma and tinospora cordifolia are reported. | 2005 | 16229969 |
| suppurative epididymo-orchitis and chronic prostatitis caused by burkholderia pseudomallei: a case report and review. | 2005 | 15996456 | |
| infected pseudoaneurysm of the femoral artery secondary to melioidosis infection of a previous femoropopliteal bypass graft. | infected arterial aneurysm or pseudoaneurysm caused by burkholderia pseudomallei is very rare; a review of the literature reveals only five previously reported cases. we report the first case of melioidosis infection of a previous femoropopliteal bypass graft complicated by para-anastomotic pseudoaneurysm of the femoral artery successfully treated by surgical removal of the old graft and creation of a new, extraanatomic, external iliac-to-popliteal bypass graft. | 2005 | 15714374 |
| detection of burkholderia pseudomallei in soil within the lao people's democratic republic. | clinical cases of melioidosis caused by the saprophyte burkholderia pseudomallei were first noted in the lao people's democratic republic (pdr) in 1999. in this study, 36% of 110 soil samples in northern lao pdr were positive for b. pseudomallei, providing further evidence for the presence of melioidosis in this country. | 2005 | 15695707 |
| epitope mapping of burkholderia pseudomallei serine metalloprotease: identification of serine protease epitope mimics. | filamentous phage random peptide libraries were used to identify the epitopes of burkholderia pseudomallei protease by panning against igg polyclonal sera that exhibited protease neutralizing properties. the isolated fusion peptides presented a consensus peptide sequence, tksmalsg, which closely resembles part of the active site sequence, 435gtsmatphvag445, of b. pseudomallei serine metalloprotease. by comparing the consensus sequence, tksmalsg, with the predicted three-dimensional molecular mod ... | 2005 | 15607634 |
| diagnosis and treatment of mycotic aneurysm due to burkholderia pseudomallei. | 2005 | 16028178 | |
| [some properties of plasmocoagulase of the causative agents of glanders and melioidosis]. | criteria for the evaluation of the plasmocoagulase activity of natural isolates and mutant strains of the causative agents of glanders and melioidosis were worked out, which made it possible to subdivide them by this sign into pathogens with high, moderate and low activity. plasmocoagulase produced by pathogenic burkholderia was shown to be a thermolabile enzyme, comparatively stable with respect to the action of such chemico-biological agents as hydrogen peroxide and chloramine. | 2005 | 16028525 |
| rapid serological diagnosis of melioidosis: an evaluation of a prototype immunochromatographic test. | to evaluate the panbio melioidosis prototype (ict) igg and igm test kits in detecting antibodies to burkholderia pseudomallei from an endemic region of australia. this would be of particular importance in high prevalence, low resource regions. | 2005 | 16028848 |
| detection and differentiation of burkholderia pseudomallei, burkholderia mallei and burkholderia thailandensis by multiplex pcr. | burkholderia pseudomallei, a gram-negative bacterium that causes melioidosis may be differentiated from closely related species of burkholderia mallei that causes glanders and non-pathogenic species of burkholderia thailandensis by multiplex pcr. the multiplex pcr consists of primers that flank a 10-bp repetitive element in b. pseudomallei and b. mallei amplifying pcr fragment of varying sizes between 400-700 bp, a unique sequence in b. thailandensis amplifying a pcr fragment of 308 bp and the m ... | 2005 | 15708316 |
| tropical medicine: melioidosis? never heard of it... | 2005 | 15815599 | |
| the capsular polysaccharide of burkholderia pseudomallei contributes to survival in serum by reducing complement factor c3b deposition. | burkholderia pseudomallei produces an extracellular polysaccharide capsule -3)-2-o-acetyl-6-deoxy-beta-d-manno-heptopyranose-(1- which has been shown to be an essential virulence determinant. the addition of purified capsule was shown to increase the virulence of a capsule mutant strain in the syrian hamster model of acute melioidosis. an increase in the number of wild-type b. pseudomallei cells in the blood was seen by 48 h, while the number of capsule mutant cells in the blood declined by 48 h ... | 2005 | 15664954 |
| cutaneous melioidosis in a man who was taken as a prisoner of war by the japanese during world war ii. | melioidosis, an infection caused by the gram-negative bacillus burkholderia pseudomallei, is endemic to southeast asia and northern australia. human infection is acquired through contact with contaminated water via percutaneous inoculation. clinical manifestations range from skin and soft tissue infection to pneumonia with sepsis. we report a case of a man who was taken as a prisoner of war by the japanese during world war ii who presented with a nonhealing ulcer on his right hand 62 years after ... | 2005 | 15695721 |
| melioidosis in mauritius. | we report the first case of human melioidosis from mauritius, where burkholderia pseudomallei has never been isolated. the patient was immunocompromised, had never traveled abroad, and had a history of regular exposure to mud. she became ill at a time when rainfall was higher than the monthly average. | 2005 | 15705340 |
| integrative genomic, transcriptional, and proteomic diversity in natural isolates of the human pathogen burkholderia pseudomallei. | natural isolates of pathogenic bacteria can exhibit a broad range of phenotypic traits. to investigate the molecular mechanisms contributing to such phenotypic variability, we compared the genomes, transcriptomes, and proteomes of two natural isolates of the gram-negative bacterium burkholderia pseudomallei, the causative agent of the human disease melioidosis. significant intrinsic genomic, transcriptional, and proteomic variations were observed between the two strains involving genes of divers ... | 2005 | 15937190 |
| [molecular-genetic approaches to diagnosis and intraspecific typing of causative agents of glanders and melioidosis]. | pathogenic burkholderia--burkholderia mallei and burkholderia pseudomallei--are causative agents of glanders and melioidosis, severe infectious diseases of man and animals. they are regarded as potential agents of bioterrorism. the existing bacteriological and immunological methods of identification of b. mallei and b. pseudomallei are not efficient enough for the rapid diagnosis and typing of strains. described in the paper are molecular methods of detection of the agents by pcr, hybridization ... | 2005 | 15954468 |
| comparison of diagnostic laboratory methods for identification of burkholderia pseudomallei. | limited experience and a lack of validated diagnostic reagents make burkholderia pseudomallei, the cause of melioidosis, difficult to recognize in the diagnostic microbiology laboratory. we compared three methods of confirming the identity of presumptive b. pseudomallei strains using a collection of burkholderia species drawn from diverse geographic, clinical, and environmental sources. the 95 isolates studied included 71 b. pseudomallei and 3 b. thailandensis isolates. the api 20ne method ident ... | 2005 | 15872242 |
| a type iv pilin, pila, contributes to adherence of burkholderia pseudomallei and virulence in vivo. | the burkholderia pseudomallei k96243 genome contains multiple type iv pilin-associated loci, including one encoding a putative pilus structural protein (pila). a pila deletion mutant has reduced adherence to human epithelial cells and is less virulent in the nematode model of virulence and the murine model of melioidosis, suggesting a role for type iv pili in b. pseudomallei virulence. | 2005 | 15664977 |
| trimethoprim/sulfamethoxazole resistance in clinical isolates of burkholderia pseudomallei. | trimethoprim/sulfamethoxazole is commonly used to treat melioidosis. antimicrobial susceptibility testing using the disc diffusion method is commonly used in melioidosis-endemic areas, but may overestimate resistance to trimethoprim/sulfamethoxazole. | 2005 | 15886263 |
| kinetic studies of bioactive products nitric oxide and 8-iso-pgf(2alpha) in burkholderia pseudomallei infected human macrophages, and their role in the intracellular survival of these organisms. | the oxidative response of burkholderia pseudomallei and escherichia coli infected macrophages from normal and melioidosis subjects was determined by measuring the production of nitric oxide which is one of the reactive nitrogen intermediates, and the activation state of these macrophages was determined by measuring the generation of 8-iso-pgf(2alpha), a bioactive product of free radical induced lipid peroxidation. macrophages obtained from the melioidosis patients generated significantly lower l ... | 2005 | 15681148 |
| community-acquired pneumonia in northern australia: low mortality in a tropical region using locally-developed treatment guidelines. | to investigate the epidemiology and outcome of adult community-acquired pneumonia (cap) in tropical australia. | 2005 | 15603991 |
| route of infection in melioidosis. | 2005 | 15834987 | |
| detection of paragonimus heterotremus eggs in experimentally infected cats by a polymerase chain reaction-based method. | a polymerase chain reaction (pcr) procedure for the detection of paragonimus heterotremus eggs in stool samples was developed and compared with stoll's egg count method. the primers were designed on the basis of a previously constructed pph-13-specific dna probe, which produced an approximate 0.5-kb amplified product. this pcr method could detect as few as 5 eggs in 0.6 g of artificially inoculated feces of a healthy control cat or as little as 1 x 10(-4) ng of p. heterotremus genomic dna. the a ... | 2005 | 15856902 |
| [burkholderia pseudomallei infection after travelling in southeast asia, case study]. | 2005 | 15780898 | |
| functional characterization of oxa-57, a class d beta-lactamase from burkholderia pseudomallei. | class d beta-lactamase oxa-57 was identified in a range of isolates of burkholderia pseudomallei and burkholderia thailandensis. comparative kinetic analyses of wild-type and mutant forms of b. pseudomallei oxa-57 are reported. implications of these data for beta-lactam resistance and the proposed role of ser-104 in beta-lactam hydrolysis are discussed. | 2005 | 15793160 |
| structural characterization of the ser324thr variant of the catalase-peroxidase (katg) from burkholderia pseudomallei. | the ser315thr variant of the catalase-peroxidase katg from mycobacterium tuberculosis imparts resistance to the pro-drug isonicotinic acid hydrazide (isoniazid) through a failure to convert it to the active drug, isonicotinoyl-nad. the equivalent variant in katg from burkholderia pseudomallei, ser324thr, has been constructed, revealing catalase and peroxidase activities that are similar to those of the native enzyme. the other activities of the variant protein, including the nadh oxidase, the is ... | 2005 | 15567407 |
| the bpsir quorum-sensing system of burkholderia pseudomallei. | bpsir, a luxir quorum-sensing homolog, is required for optimal expression of virulence and secretion of exoproducts in burkholderia pseudomallei. cell density-dependent expression of bpsi and bpsr, the positive regulation of bpsir expression by bpsr, and the synthesis of n-octanoyl-homoserine lactone (c8hsl) by bpsi are described in this report. | 2005 | 15629951 |
| melioidosis imported into nepal. | this is a report of the first recognized case of melioidosis in nepal. illness began 1 month after returning from malaysia after a 1 y stay. the case highlights the importance of ascertaining the travel history in any patient with a suspected infectious disease in this age of global travel. | 2005 | 15764193 |
| type iii secretion system cluster 3 is required for maximal virulence of burkholderia pseudomallei in a hamster infection model. | burkholderia pseudomallei, the etiological agent of melioidosis, is an animal pathogen capable of inducing a highly fatal septicemia. b. pseudomallei possesses three type iii secretion system (ttss) clusters, two of which (ttss1 and ttss2) are homologous to the ttss of the plant pathogen ralstonia solanacearum, and one (ttss3) is homologous to the salmonella spi-1 mammalian pathogenicity island. we have demonstrated that ttss3 is required for the full virulence of b. pseudomallei in a hamster mo ... | 2005 | 15621426 |
| pulmonary melioidosis associated with bronchiolitis obliterans organizing pneumonia. | we report a case of pulmonary melioidosis associated bronchiolitis obliterans with organizing pneumonia (boop). while antibiotic treatment alone did not show any major effect, addition of corticosteroids resulted in rapid clinical improvement. to our knowledge this is the first documented case of melioidosis associated with organising pneumonia. the possible therapeutic implications for the treatment of severe pulmonary melioidosis are discussed. | 2005 | 15603844 |
| relative uptake of technetium 99m stannous colloid by neutrophils and monocytes is altered by gram-negative infection. | gram-negative infection alters phagocytic cell function; hence, it could affect phagocytic uptake of inorganic colloids by these cells. neutrophil and monocyte uptake of technetium 99m stannous colloid (99mtc snc) in whole blood was measured in 10 patients with gram-negative infection (burkholderia pseudomallei) and 7 controls. mean uptake per individual neutrophil was reduced in infection. uptake per monocyte was not significantly different. blood from six normal individuals was incubated with ... | 2005 | 15691667 |
| brothers in arms. | 2005 | 15693175 | |
| actin-based motility of intracellular pathogens. | the actin cytoskeleton is harnessed by several pathogenic bacteria that are capable of entering into non-phagocytic cells, the so-called 'invasive bacteria'. among them, a few also exploit the host actin cytoskeleton to move intra- and inter-cellularly. our knowledge of the basic mechanisms underlying actin-based motility has dramatically increased and the list of bacteria that are able to move in this way is also increasing including not only listeria, shigella and rickettsia species but also m ... | 2005 | 15694855 |
| rapid presumptive identification of burkholderia pseudomallei with real-time pcr assays using fluorescent hybridization probes. | burkholderia pseudomallei (the etiologic agent of melioidosis) can cause pyogenic or granulomatous lesions in almost any organ. septicemia has a case fatality rate of >40%. early diagnosis and appropriate antibiotic therapy are crucial for survival, but cultivation, biochemical identification, and conventional pcr of b. pseudomallei are time consuming. we established real-time pcr assays using fluorescent hybridization probes targeting the 16s rdna, the flagellin c (flic) and the ribosomal prote ... | 2005 | 15652215 |
| preliminary evaluation of the api 20ne and rapid nf plus systems for rapid identification of burkholderia pseudomallei and b. mallei. | we evaluated the api 20ne and the rapid nf plus systems with 58 burkholderia pseudomallei and 23 b. mallei strains for identification of these agents, but neither was reliable for confirmatory identification, with only 0 to 60% strains identified accurately. a greater diversity of strains in the system databases would be beneficial. | 2005 | 15635021 |
| [first description of an autopsied case of melioidosis in ceará state]. | an autopsied case is reported concerning fulminant sepsis with predominantly pulmonary lesions, caused by burkholderia pseudomallei, etiologic agent of melioidosis, originating from interior of ceará state, northeastern brazil where there had been no previous cases reported. autopsy findings and differential diagnosis are discussed. | 2005 | 15717097 |
| neutralization of burkholderia pseudomallei protease by fabs generated through phage display. | the isolation of therapeutic and functional protease inhibitors in vitro via combinatorial chemistry and phage display technology has been described previously. here we report the construction of a combinatorial mouse-human chimeric antibody fragment (fab) antibody library targeted against the protease of the tropical pathogen, burkholderia pseudomallei. the resulting library was biopanned against the protease, and selected clones were analyzed for their ability to function as protease inhibitor ... | 2005 | 16377887 |
| an electronmicroscopic study of the interaction of burkholderia pseudomallei and human macrophages. | b. pseudomallei has been shown to persist intracellularly in melioidosis patients until reactivated by decreasing immunocompetence. we have shown by transmission electron microscopy the internalization of b. pseudomallei by human macrophages via conventional phagocytosis enclosed within membrane-bound vacuoles or phagosomes. ferritin labeled lysosomes provided evidence of phagosome-lysosome fusion. ingested bacilli were designated as "intact" or "damaged" on the basis of their ultrastructural fe ... | 2005 | 16676686 |
| burkholderia pseudomallei infections in finnish tourists injured by the december 2004 tsunami in thailand. | 2005 | 16702634 | |
| short report: application of a polymerase chain reaction to detect burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis. | the diagnostic potential of a burkholderia pseudomallei type three secretion system (tts1) polymerase chain reaction (pcr) was examined on clinical specimens from 27 patients with sepsis in the northern territory of australia, a region endemic for melioidosis. the tts1 pcr was conducted on dna extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). the pcr sensitivity in culture-positive cli ... | 2005 | 16354831 |
| seroprevalence of burkholderia pseudomallei in east timorese refugees: implications for healthcare in east timor. | melioidosis is a disease with protean clinical manifestations caused by the bacterium burkholderia pseudomallei. it is endemic in countries surrounding the newly independent east timor, but has yet to be isolated or demonstrated serologically in that country. one illness that can be clinically indistinguishable from melioidosis is pulmonary tuberculosis, a condition with a very high prevalence in east timor. we used an indirect hemagglutination test (iha) to measure antibodies to b. pseudomallei ... | 2005 | 16610652 |
| melioidosis in tsunami survivors. | 2005 | 16355505 | |
| melioidosis: the johor bahru experience. | a 5 year retrospective review of cases of melioidosis was carried out in sultanah aminah hospital, johor bahru. there were 44 new cases of melioidosis which was proven by either blood or pus culture growing burkholderia pseudomallei from the period between january 1999 and december 2003. of these, 38 (86.4%) were males compared to only 6 (13.6%) females. thirty-one (70.5%) were malays, 7 (15.9%) were chinese, 5 (11.4%) were indians and 1 (2.2%) was a sarawakian. the peak age group was between 50 ... | 2005 | 16515111 |
| recurrent melioidosis in patients in northeast thailand is frequently due to reinfection rather than relapse. | human melioidosis is associated with a high rate of recurrent disease, despite adequate antimicrobial treatment. here, we define the rate of relapse versus the rate of reinfection in 116 patients with 123 episodes of recurrent melioidosis who were treated at sappasithiprasong hospital in northeast thailand between 1986 and 2005. pulsed-field gel electrophoresis was performed on all isolates; isolates from primary and recurrent disease for a given patient different by one or more bands were exami ... | 2005 | 16333094 |
| burkholderia pseudomallei-induced expression of suppressor of cytokine signaling 3 and cytokine-inducible src homology 2-containing protein in mouse macrophages: a possible mechanism for suppression of the response to gamma interferon stimulation. | burkholderia pseudomallei, the causative agent of melioidosis, is a facultative intracellular gram-negative bacterium that is able to survive and multiply in macrophages. previously, we reported that b. pseudomallei was able to escape macrophage killing by interfering with the expression of inducible nitric oxide synthase (inos). in the present study, we extended this finding and demonstrated that b. pseudomallei was able to activate the expression of suppressor of cytokine signaling 3 (socs3) a ... | 2005 | 16239531 |
| [ultrastructural immunochemical study of pathogenic burkholderia capsule]. | the capsular structures of burkholderia pseudomallei, b. mallei, b. cepacia and their avirulent noncapsular mutants were studied with the use of electron ahd immunocytochemical techniques. for this purpose, antimelio-idosis monoclonal antibodies (mcab) g11 and 1 g2, epitope-aimed at capsular glycopyotein of 200 kd and outer-membrane proteins of 42 and 39 kd, were used. as revealed in this study, the typical causative agents of melioidosis and glanders formed the capsule and exhibited high virule ... | 2005 | 16279528 |
| the identification and characterization of two phosphatidylinositol-4,5-bisphosphate 4-phosphatases. | numerous inositol polyphosphate 5-phosphatases catalyze the degradation of phosphatidylinositol-4,5-bisphosphate (ptdins-4,5-p(2)) to phosphatidylinositol-4-phosphate (ptdins-4-p). an alternative pathway to degrade ptdins-4,5-p(2) is the hydrolysis of ptdins-4,5-p(2) by a 4-phosphatase, leading to the production of ptdins-5-p. whereas the bacterial ipgd enzyme is known to catalyze this reaction, no such mammalian enzyme has been found. we have identified and characterized two previously undescri ... | 2005 | 16365287 |
| pyogenic vertebral osteomyelitis: a review of 14 cases. | to review and evaluate the management of 14 patients with pyogenic vertebral osteomyelitis at national university hospital, singapore between 1998 and 2001. | 2005 | 16365486 |
| rapid identification of burkholderia pseudomallei in blood culture supernatants by a coagglutination assay. | in total, 309 blood culture supernatants were tested for the presence of burkholderia pseudomallei antigen using an in-house coagglutination test prepared by sensitising cowan i staphylococcal cells with b. pseudomallei polyclonal antiserum. the coagglutination test gave a sensitivity, specificity, positive predictive value and negative predictive value of 100% in comparison with blood culture. a subset of 102 supernatants was also tested for b. pseudomallei antigen using a monoclonal antibody-b ... | 2005 | 16216112 |
| the role and significance of sputum cultures in the diagnosis of melioidosis. | pneumonia is a common manifestation of melioidosis, the disease caused by burkholderia pseudomallei. in this study, we defined the prognostic significance of a positive sputum culture. a total of 712 patients presenting to sappasithiprasong hospital, ubon ratchathani, thailand, with melioidosis between january 1992 and december 2002 had a sputum culture performed during admission, which was positive for b. pseudomallei in 444 patients (62%). the median duration of sputum positivity was 9 days (r ... | 2005 | 16222004 |
| genome-wide expression analysis of iron regulation in burkholderia pseudomallei and burkholderia mallei using dna microarrays. | burkholderia pseudomallei and b. mallei are the causative agents of melioidosis and glanders, respectively. as iron regulation of gene expression is common in bacteria, in the present studies, we have used microarray analysis to examine the effects of growth in different iron concentrations on the regulation of gene expression in b. pseudomallei and b. mallei. gene expression profiles for these two bacterial species were similar under high and low iron growth conditions irrespective of growth ph ... | 2005 | 16242861 |
| serodiagnosis of melioidosis by a competitive enzyme-linked immunosorbent assay using a lipopolysaccharide-specific monoclonal antibody. | burkholderia pseudomallei is the causative agent of melioidosis, a severe and potentially fatal infectious disease in humans known to be endemic in southeast asia and northern australia. the infection is also increasingly recognized in various animal species with a potential to spread to humans. with the potential as a biological warfare agent, specific serodiagnosis of melioidosis for surveillance in large populations at risk, humans or animals, would be highly valuable. in this study, a compet ... | 2005 | 16252843 |
| in disaster's wake: tsunami lung. | 2005 | 16263492 | |
| open-label randomized trial of oral trimethoprim-sulfamethoxazole, doxycycline, and chloramphenicol compared with trimethoprim-sulfamethoxazole and doxycycline for maintenance therapy of melioidosis. | melioidosis (infection caused by burkholderia pseudomallei) requires a prolonged course of oral antibiotics following initial intravenous therapy to reduce the risk of relapse after cessation of treatment. the current recommendation is a four-drug regimen (trimethoprim [tmp], sulfamethoxazole [smx], doxycycline, and chloramphenicol) and a total treatment time of 12 to 20 weeks. drug side effects are common; the aim of this study was to compare the efficacy and tolerance of the four-drug regimen ... | 2005 | 16189075 |
| the burkholderia pseudomallei bpeab-oprb efflux pump: expression and impact on quorum sensing and virulence. | bpeab-oprb is a multidrug efflux pump of the bacterial pathogen burkholderia pseudomallei and is responsible for conferring antimicrobial resistance to aminoglycosides and macrolides. expression of bpeab-oprb is inducible by its substrate erythromycin and upon entry into stationary phase. bper, a member of the tetr family, functions as a repressor of the bpeab-oprb operon. bper expression was similarly induced at stationary phase but lagged behind the induction of bpeab-oprb expression. the indu ... | 2005 | 15995185 |
| pediatric melioidosis in pahang, malaysia. | melioidosis is much less common in children than in adults. this study investigated the incidence, demographic characteristics, presenting symptoms and outcome of pediatric melioidosis in pahang, malaysia. this retrospective study included patients < or =18 years old with positive body fluid cultures for burkholderia pseudomallei from january 2000 to june 2003. data on culture results were obtained from 2 referral hospitals. the incidence of pediatric melioidosis was 0.68/100,000 population per ... | 2005 | 16211138 |
| humoral and cell-mediated adaptive immune responses are required for protection against burkholderia pseudomallei challenge and bacterial clearance postinfection. | burkholderia pseudomallei, the causative agent of melioidosis, is a gram-negative bacillus endemic to areas of southeast asia and northern australia. presently, there is no licensed vaccine for b. pseudomallei and the organism is refractive to antibiotic therapy. the bacterium is known to survive and multiply inside both phagocytic and nonphagocytic host cells and may be able to spread directly from cell to cell. current vaccine delivery systems are unlikely to induce the correct immune effector ... | 2005 | 16113315 |
| melioidosis and the vascular surgeon: hospital kuala lumpur experience. | bacterial arteritis is relatively uncommon and management of this condition, which carries high morbidity and mortality, is difficult and time-consuming. common organisms implicated include salmonella and staphylococcus. arteritis as a result of infection by burkholderia pseudomallei (formerly pseudomonas pseudomallei) has been rarely reported in the english literature. this organism, which is endemic in our part of the world, is well known to cause a wide spectrum of septic conditions. a review ... | 2005 | 16234087 |
| short report: disease severity and outcome of melioidosis in hiv coinfected individuals. | this study examined whether coinfection with hiv and burkholderia pseudomallei leads to altered disease severity or outcome associated with melioidosis. coinfection was detected in only 8 of 524 (1.5%) adults with melioidosis in northeast thailand. clinical presentation and acute outcome were similar in hiv-positive and hiv-negative patients. | 2005 | 16354832 |
| attempted passive prophylaxis with a monoclonal anti-burkholderia pseudomallei exopolysaccharide antibody in a murine model of melioidosis. | melioidosis is a severe gram-negative infection caused by the facultative intracellular bacterium burkholderia pseudomallei, which is responsible for a broad spectrum of symptoms in both humans and animals. no licensed vaccine currently exists. this study evaluated the protective effect of a monoclonal antibody (mab ps6f6) specific to b. pseudomallei exopolysaccharide in an outbred murine model of sub-acute melioidosis. when administered before the infectious challenge, ps6f6 significantly incre ... | 2005 | 16435577 |
| the effect of methanolic extract of tamarindus indica linn. on the growth of clinical isolates of burkholderia pseudomallei. | burkholderia pseudomallei (pseudomonas pseudomallei) causes melioidosis, a life-threatening infection common among paddy cultivators in southeast asian countries. no plant materials have been investigated for its activity against b. pseudomallei. therefore, a preliminary study was carried out using disc diffusion and minimum inhibitory concentration (mic) methods to evaluate the anti-b. pseudomallei activity of five indian medicinal plants documented to have been used for several ailments in the ... | 2005 | 16518004 |
| burkholderia oklahomensis sp. nov., a burkholderia pseudomallei-like species formerly known as the oklahoma strain of pseudomonas pseudomallei. | c6786, the clinical isolate of the 'oklahoma' strain of pseudomonas (now burkholderia) pseudomallei, was originally isolated in 1973 from a wound infection resulting from a farming accident in oklahoma, usa. environmental isolates c7532 and c7533 from the oklahoma accident site were found to match c6786. these three isolates and a clinical isolate originally identified as b. pseudomallei that was recovered from a person in georgia, usa, involved in an automobile accident were characterized by bi ... | 2006 | 16957116 |
| environmental factors that affect the survival and persistence of burkholderia pseudomallei. | 2006 | 16980433 | |
| melioidosis: an imported case from madagascar. | burkholderia pseudomallei, the causative agent of melioidosis, is endemic in southeast asia and northern australia but is a rare pathogen in other parts of the world. no human case of melioidosis has been reported in madagascar until now. we describe a case of pulmonary melioidosis probably acquired in madagascar. | 2006 | 16987131 |
| use of a variable amplicon typing scheme reveals considerable variation in the accessory genomes of isolates of burkholderia pseudomallei. | melioidosis, a disease caused by the bacterium burkholderia pseudomallei, is endemic in southeast asia and northern australia. we used suppression subtractive hybridization (ssh) to identify sequences that varied between two b. pseudomallei isolates from australia and determined the distribution of 45 ssh-derived sequences among a panel of b. pseudomallei and b. thailandensis isolates. sequences exhibiting variable prevalence were included in a variable amplicon typing (vat) scheme designed to s ... | 2006 | 16597858 |
| genome-wide expression analysis of burkholderia pseudomallei infection in a hamster model of acute melioidosis. | burkholderia pseudomallei is the causative agent of melioidosis and represents a potential bioterrorism threat. in the current studies we have examined gene expression in b. pseudomallei in an animal model of acute melioidosis using whole-genome microarrays. gene expression profiles were generated by comparing transcriptional levels of b. pseudomallei-expressed genes in infected hamster organs including liver, lung, and spleen following intraperitoneal and intranasal routes of infection to those ... | 2006 | 16988221 |
| roles for arg426 and trp111 in the modulation of nadh oxidase activity of the catalase-peroxidase katg from burkholderia pseudomallei inferred from ph-induced structural changes. | crystals of burkholderia pseudomallei katg retain their ability to diffract x-rays at high resolution after adjustment of the ph from 5.6 to 4.5, 6.5, 7.5, and 8.5, providing a unique view of the effect of ph on protein structure. one significant ph-sensitive change lies in the appearance of a perhydroxy group attached to the indole nitrogen of the active site trp111 above ph 7, similar to a modification originally observed in the ser324thr variant of the enzyme at ph 5.6. the modification forms ... | 2006 | 16618106 |
| role of inducible nitric oxide synthase and nadph oxidase in early control of burkholderia pseudomallei infection in mice. | infection with the soil bacterium burkholderia pseudomallei can result in a variety of clinical outcomes, including asymptomatic infection. the initial immune defense mechanisms which might contribute to the various outcomes after environmental contact with b. pseudomallei are largely unknown. we have previously shown that relatively resistant c57bl/6 mice can restrict bacterial b. pseudomallei growth more efficiently within 1 day after infection than highly susceptible balb/c mice. by using thi ... | 2006 | 17000727 |
| genomic fingerprinting of burkholderia pseudomallei and b. mallei pathogens with dna array based on interspecies sequence differences obtained by subtractive hybridization. | the ability to rapidly and efficiently identify causative agents of dangerous human and animal diseases is a prerequisite to diagnosis, prophylaxis and therapy. such identification systems can be developed based on dna markers enabling differentiation between various bacterial strains. one source of these markers is genetic polymorphism. an efficient method for detecting the most stable polymorphisms without knowledge of genomic sequences is subtractive hybridization. in this work we report an a ... | 2006 | 16621455 |
| method for regulated expression of single-copy efflux pump genes in a surrogate pseudomonas aeruginosa strain: identification of the bpeef-oprc chloramphenicol and trimethoprim efflux pump of burkholderia pseudomallei 1026b. | construction and integration of recombinant mini-tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. using this method, the burkholderia pseudomallei bpeef-oprc operon was shown to encode a chloramphenicol and trimethoprim efflux pump. | 2006 | 17005832 |
| melioidosis in an italian tourist injured by the tsunami in thailand. | 2006 | 17024805 | |
| endotoxin-like properties of a rhamnolipid exotoxin from burkholderia (pseudomonas) plantarii: immune cell stimulation and biophysical characterization. | here we report on the purification, structural characterization, and biological activity of a glycolipid, 2-o-alpha-l-rhamnopyranosyl-alpha-l-rhamnopyranosyl-alpha(r)-3-hydroxytetradecanoyl-(r)-3-hydroxytetradecanoate (rl-2,2(14)) produced by burkholderia (pseudomonas) plantarii. rl-2,2(14) is structurally very similar to a rhamnolipid exotoxin from pseudomonas aeruginosa and identical to the rhamnolipid of burkholderia pseudomallei, the causative agent of melioidosis. interestingly, rl-2,2(14) ... | 2006 | 16542152 |
| solution structure of monomeric bsal, the type iii secretion needle protein of burkholderia pseudomallei. | many gram-negative bacteria that are important human pathogens possess type iii secretion systems as part of their required virulence factor repertoire. during the establishment of infection, these pathogens coordinately assemble greater than 20 different proteins into a macromolecular structure that spans the bacterial inner and outer membranes and, in many respects, resembles and functions like a syringe. this type iii secretion apparatus (ttsa) is used to inject proteins into a host cell's me ... | 2006 | 16631790 |
| in vitro activity of tigecycline against burkholderia pseudomallei and burkholderia thailandensis. | investigation of the in vitro activity of tigecycline against burkholderia pseudomallei and burkholderia thailandensis revealed that the inhibition zone diameters of tigecycline against all isolates were >or=20 mm and that the mic50 values were 0.5 and 1 microg/ml and the mic90 values were 2 and 1.5 microg/ml for b. pseudomallei and b. thailandensis, respectively. | 2006 | 16569883 |
| global map of growth-regulated gene expression in burkholderia pseudomallei, the causative agent of melioidosis. | many microbial pathogens express specific virulence traits at distinct growth phases. to understand the molecular pathways linking bacterial growth to pathogenicity, we have characterized the growth transcriptome of burkholderia pseudomallei, the causative agent of melioidosis. using a fine-scale sampling approach, we found approximately 17% of all b. pseudomallei genes displaying regulated expression during growth in rich medium, occurring as broad waves of functionally coherent gene expression ... | 2006 | 16997946 |
| activity of tigecycline in the treatment of acute burkholderia pseudomallei infection in a murine model. | burkholderia pseudomallei is the causative agent of melioidosis. standard therapy includes ceftazidime alone or in combination with co-trimoxazole. tigecycline, a novel agent, has displayed activity against b. pseudomallei. we evaluated the in vivo efficacy of tigecycline using a murine model of melioidosis. mice were infected with either a high or low virulence b. pseudomallei isolate followed by administration of antibiotics alone or in combination (tigecycline, ceftazidime, tigecycline plus c ... | 2006 | 17046208 |
| management of melioidosis. | melioidosis is a serious human infection caused by the environmental gram-negative bacterium burkholderia pseudomallei. outcome following melioidosis remains poor despite 20 years of clinical research. overall mortality is 50% in north-east thailand (35% in children) and 19% in australia. relapse is common (13% over 10 years), and results from failure to eradicate the organism. treatment is required to complete 12-20 weeks, or longer if clinically indicated. this is divided into intravenous and ... | 2006 | 16771621 |
| cutaneous melioidosis. | melioidosis is an infection caused by the gram-negative bacterium burkholderia pseudomallei. this disease is endemic in southeast asia and north australia with sporadic occurrence in temperate countries, mostly imported from travellers. any organ can be involved in melioidosis whereby burkholderia pseudomallei causes an acute inflammatory reaction with rapid development of small abcesses which tend to coalesce to form larger abscesses. cutaneous manifestations vary greatly. we report a man with ... | 2006 | 17062053 |
| in silico analysis of burkholderia pseudomallei genome sequence for potential drug targets. | recent advances in dna sequencing technology have enabled elucidation of whole genome information from a plethora of organisms. in parallel with this technology, various bioinformatics tools have driven the comparative analysis of the genome sequences between species and within isolates. while drawing meaningful conclusions from a large amount of raw material, computer-aided identification of suitable targets for further experimental analysis and characterization, has also led to the prediction ... | 2006 | 16922696 |
| characterization of the mrgrs locus of the opportunistic pathogen burkholderia pseudomallei: temperature regulates the expression of a two-component signal transduction system. | burkholderia pseudomallei is a saprophyte in tropical environments and an opportunistic human pathogen. this versatility requires a sensing mechanism that allows the bacterium to respond rapidly to altered environmental conditions. we characterized a two-component signal transduction locus from b. pseudomallei 204, mrgr and mrgs, encoding products with extensive homology with response regulators and histidine protein kinases of escherichia coli, bordetella pertussis, and vibrio cholerae. | 2006 | 16893462 |
| six cases of suppurative lymphadenitis caused by burkholderia pseudomallei infection. | melioidosis, an infection caused by burkholderia pseudomallei, is endemic in southeast asia and northern australia. it can have various presentations, ranging from acute septicaemia to chronic localized infection. uncomplicated, localized infection usually involves skin or subcutaneous tissues. suppurative lymphadenitis caused by melioidosis has been rarely encountered by clinicians practicing in endemic areas. we encountered six cases of suppurative lymphadenitis caused by burkholderia pseudoma ... | 2006 | 16542691 |
| the innate interferon gamma response of balb/c and c57bl/6 mice to in vitro burkholderia pseudomallei infection. | burkholderia pseudomallei is the causative agent for melioidosis. for many bacterial infections, cytokine dysregulation is one of the contributing factors to the severe clinical outcomes in the susceptible hosts. the c57bl/6 and balb/c mice have been established as a differential model of susceptibility in murine melioidosis. in this study, we compared the innate ifn-gamma response to b. pseudomallei between the c57bl/6 and balb/c splenocytes and characterized the hyperproduction of ifn-gamma in ... | 2006 | 16919160 |
| development of a polymerase chain reaction assay for the specific identification of burkholderia mallei and differentiation from burkholderia pseudomallei and other closely related burkholderiaceae. | burkholderia mallei and burkholderia pseudomallei, the etiologic agents responsible for glanders and melioidosis, respectively, are genetically and phenotypically similar and are category b biothreat agents. we used an in silico approach to compare the b. mallei atcc 23344 and b. pseudomallei k96243 genomes to identify nucleotide sequences unique to b. mallei. five distinct b. mallei dna sequences and/or genes were identified and evaluated for polymerase chain reaction (pcr) assay development. g ... | 2006 | 16546342 |
| temperature-regulated microcolony formation by burkholderia pseudomallei requires pila and enhances association with cultured human cells. | burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease that is endemic to northern australia and southeast asia and is acquired from soil or water. adherence of b. pseudomallei 08 to cultured cells increases dramatically following prior growth at 30 degrees c or less compared to that following prior growth at 37 degrees c. here, we show that this occurs almost entirely as the result of microcolony formation (bacterium-bacterium interactions) following growth ... | 2006 | 16926432 |