Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| virulence of burkholderia pseudomallei does not correlate with biofilm formation. | burkholderia pseudomallei is the causative agent of melioidosis but currently the pathogenesis of the disease is still poorly understood. one of the virulent factors of gram-negative bacteria is the ability to produce biofilm to evade host defense. as b. pseudomallei has also been reported to develop the biofilm [1], in the present study, we therefore, quantified the biofilm formation in 50 strains of b. pseudomallei and compared with 50 strains of its avirulent counterpart burkholderia thailand ... | 2005 | 16084684 |
| fatal septicemic melioidosis in a young military person possibly co-infected with leptospira interrogans and orientia tsutsugamushi. | concurrent melioidosis, leptospirosis, and scrub typhus after rural activities is rarely reported. a 19-year-old previously healthy man had fever onset after 2 weeks of military training. pneumonia became evident on the fifth day of fever under intravenous penicillin and oral minocycline therapy. acute respiratory failure developed the next day with shock and acute renal and liver function deterioration, which resulted in death. blood cultures on the third and fifth days grew burkholderia pseudo ... | 2005 | 15909673 |
| [molecular-genetic approaches to diagnosis and intraspecific typing of causative agents of glanders and melioidosis]. | pathogenic burkholderia--burkholderia mallei and burkholderia pseudomallei--are causative agents of glanders and melioidosis, severe infectious diseases of man and animals. they are regarded as potential agents of bioterrorism. the existing bacteriological and immunological methods of identification of b. mallei and b. pseudomallei are not efficient enough for the rapid diagnosis and typing of strains. described in the paper are molecular methods of detection of the agents by pcr, hybridization ... | 2005 | 15954468 |
| treatment of survivors after the tsunami. | 2005 | 15972880 | |
| melioidosis in tsunami survivors. | 2005 | 16355505 | |
| modeling of an ion channel in its open conformation. | we have modeled the structure of kirbac1.1 in an open state using as a starting point the structure of kirbac1.1 in its closed conformation (protein data bank 1p7b). to test the validity of the open-state model, molecular dynamics simulations in octane, a lipid bilayer mimetic, were carried out. simulations of the closed conformer were used for comparison purposes. the total simulation time was approximately 138 ns. the initial open model was refined by using projection maps obtained from electr ... | 2005 | 15985423 |
| pyogenic vertebral osteomyelitis: a review of 14 cases. | to review and evaluate the management of 14 patients with pyogenic vertebral osteomyelitis at national university hospital, singapore between 1998 and 2001. | 2005 | 16365486 |
| the burkholderia pseudomallei bpeab-oprb efflux pump: expression and impact on quorum sensing and virulence. | bpeab-oprb is a multidrug efflux pump of the bacterial pathogen burkholderia pseudomallei and is responsible for conferring antimicrobial resistance to aminoglycosides and macrolides. expression of bpeab-oprb is inducible by its substrate erythromycin and upon entry into stationary phase. bper, a member of the tetr family, functions as a repressor of the bpeab-oprb operon. bper expression was similarly induced at stationary phase but lagged behind the induction of bpeab-oprb expression. the indu ... | 2005 | 15995185 |
| neutralization of burkholderia pseudomallei protease by fabs generated through phage display. | the isolation of therapeutic and functional protease inhibitors in vitro via combinatorial chemistry and phage display technology has been described previously. here we report the construction of a combinatorial mouse-human chimeric antibody fragment (fab) antibody library targeted against the protease of the tropical pathogen, burkholderia pseudomallei. the resulting library was biopanned against the protease, and selected clones were analyzed for their ability to function as protease inhibitor ... | 2005 | 16377887 |
| interaction between burkholderia pseudomallei and the host immune response: sleeping with the enemy? | melioidosis is an infectious disease endemic in tropical and subtropical areas but is most often described in southeast asia and northern australia. it has increasingly gained attention in the western hemisphere because of its potential use as a biological weapon. progress in our understanding of the virulence mechanisms of burkholderia pseudomallei, the causative agent, has provided clues to the pathogenesis of the disease. the bacterium can remain latent in the body for long periods of time an ... | 2005 | 16235187 |
| melioidosis, northeastern brazil. | melioidosis was first recognized in northeastern brazil in 2003. confirmation of additional cases from the 2003 cluster in ceará, more recent cases in other districts, environmental isolation of burkholderia pseudomallei, molecular confirmation and typing results, and positive serosurveillance specimens indicate that melioidosis is more widespread in northeastern brazil than previously thought. | 2005 | 16229782 |
| antimicrobial activity of some medicinal plants from india. | the results of a preliminary antimicrobial screening of the methanol extracts of zingiber officinale, asteracantha longifolia, citrus acida, salacia microsperma and tinospora cordifolia are reported. | 2005 | 16229969 |
| melioidosis in new caledonia. | recognized melioidosis-endemic areas are widening. in the south pacific, melioidosis is endemic in new caledonia, northern australia, and papua new guinea. we report the first 4 documented cases of human melioidosis from new caledonia. molecular typing of 2 burkholderia pseudomallei isolates suggests a link to australian strains. | 2005 | 16318706 |
| trimethoprim/sulfamethoxazole resistance in clinical isolates of burkholderia pseudomallei. | trimethoprim/sulfamethoxazole is commonly used to treat melioidosis. antimicrobial susceptibility testing using the disc diffusion method is commonly used in melioidosis-endemic areas, but may overestimate resistance to trimethoprim/sulfamethoxazole. | 2005 | 15886263 |
| recurrent melioidosis in patients in northeast thailand is frequently due to reinfection rather than relapse. | human melioidosis is associated with a high rate of recurrent disease, despite adequate antimicrobial treatment. here, we define the rate of relapse versus the rate of reinfection in 116 patients with 123 episodes of recurrent melioidosis who were treated at sappasithiprasong hospital in northeast thailand between 1986 and 2005. pulsed-field gel electrophoresis was performed on all isolates; isolates from primary and recurrent disease for a given patient different by one or more bands were exami ... | 2005 | 16333094 |
| rapid immunofluorescence microscopy for diagnosis of melioidosis. | an immunofluorescent (if) method that detects burkholderia pseudomallei in clinical specimens within 10 min was devised. the results of this rapid method and those of an existing if method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. the sensitivities of both if tests were 66%, and the specificities were 99.5 and 99.4%, respectively. | 2005 | 15817767 |
| bacterial genome adaptation to niches: divergence of the potential virulence genes in three burkholderia species of different survival strategies. | two closely related species burkholderia mallei (bm) and burkholderia pseudomallei (bp) are serious human health hazards and are potential bio-warfare agents, whereas another closely related species burkholderia thailandensis (bt) is a non-pathogenic saprophyte. to investigate the genomic factors resulting in such a dramatic difference, we first identified the bm genes responsive to the mouse environment, and then examined the divergence of these genes in bp and bt. | 2005 | 16336651 |
| disinfection of burkholderia pseudomallei in potable water. | the effect of chlorine, monochloramine and uv disinfection on the water-borne pathogen burkholderia pseudomallei was assessed. persistence of b. pseudomallei was verified by mpn involving a one-step recovery procedure. chlorine proved the most effective disinfectant with a 99.99% reduction of a 10(6) cfu/ml pure bacterial culture followed by 99.9% reduction by monochloramine and 99% reduction by uv. co-culture of b. pseudomallei with acanthamoeba astronyxis was found to greatly enhance survival ... | 2005 | 15766962 |
| identification of a bacterial factor required for actin-based motility of burkholderia pseudomallei. | burkholderia pseudomallei is a gram-negative facultative intracellular pathogen that enters and escapes from eukaryotic cells using the power of actin polymerization. we have identified a bacterial protein (bima) that is required for the ability of b. pseudomallei to induce the formation of actin tails. bima contains proline-rich motifs and wh2-like domains and shares limited homology at the c-terminus with the yersinia autosecreted adhesin yada. bima is located at the pole of the bacterial cell ... | 2005 | 15773977 |
| tsunami lung: a necrotising pneumonia in survivors of the asian tsunami. | 2005 | 15804231 | |
| [melioidosis]. | melioidosis is an emerging zoonosis, due to burkholderia pseudomallei, which is a highly invasive, resistant, and resilient soil bacteria, transmitted by cutaneous or airborne route, and is a potential weapon for bioterrorism. although the agent has been identified all over the world, the human disease is endemic only in se asia and northern australia, and gained recent interest after the december 2004 tsunami. human infection can be a very severe systemic disease (mortality 20 to 80%), with pro ... | 2005 | 16253457 |
| tropical medicine: melioidosis? never heard of it... | 2005 | 15815599 | |
| actin-binding proteins from burkholderia mallei and burkholderia thailandensis can functionally compensate for the actin-based motility defect of a burkholderia pseudomallei bima mutant. | recently we identified a bacterial factor (bima) required for actin-based motility of burkholderia pseudomallei. here we report that burkholderia mallei and burkholderia thailandensis are capable of actin-based motility in j774.2 cells and that bima homologs of these bacteria can restore the actin-based motility defect of a b. pseudomallei bima mutant. while the bima homologs differ in their amino-terminal sequence, they interact directly with actin in vitro and vary in their ability to bind arp ... | 2005 | 16267310 |
| use of a real-time pcr taqman assay for rapid identification and differentiation of burkholderia pseudomallei and burkholderia mallei. | a taqman allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype burkholderia pseudomallei and burkholderia mallei isolates. the assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as burkholderia thailandensis and burkholderia cepacia. | 2005 | 16272516 |
| [ultrastructural immunochemical study of pathogenic burkholderia capsule]. | the capsular structures of burkholderia pseudomallei, b. mallei, b. cepacia and their avirulent noncapsular mutants were studied with the use of electron ahd immunocytochemical techniques. for this purpose, antimelio-idosis monoclonal antibodies (mcab) g11 and 1 g2, epitope-aimed at capsular glycopyotein of 200 kd and outer-membrane proteins of 42 and 39 kd, were used. as revealed in this study, the typical causative agents of melioidosis and glanders formed the capsule and exhibited high virule ... | 2005 | 16279528 |
| rapid presumptive identification of burkholderia pseudomallei with real-time pcr assays using fluorescent hybridization probes. | burkholderia pseudomallei (the etiologic agent of melioidosis) can cause pyogenic or granulomatous lesions in almost any organ. septicemia has a case fatality rate of >40%. early diagnosis and appropriate antibiotic therapy are crucial for survival, but cultivation, biochemical identification, and conventional pcr of b. pseudomallei are time consuming. we established real-time pcr assays using fluorescent hybridization probes targeting the 16s rdna, the flagellin c (flic) and the ribosomal prote ... | 2005 | 15652215 |
| suppurative epididymo-orchitis and chronic prostatitis caused by burkholderia pseudomallei: a case report and review. | 2005 | 15996456 | |
| diagnosis and treatment of mycotic aneurysm due to burkholderia pseudomallei. | 2005 | 16028178 | |
| [some properties of plasmocoagulase of the causative agents of glanders and melioidosis]. | criteria for the evaluation of the plasmocoagulase activity of natural isolates and mutant strains of the causative agents of glanders and melioidosis were worked out, which made it possible to subdivide them by this sign into pathogens with high, moderate and low activity. plasmocoagulase produced by pathogenic burkholderia was shown to be a thermolabile enzyme, comparatively stable with respect to the action of such chemico-biological agents as hydrogen peroxide and chloramine. | 2005 | 16028525 |
| rapid serological diagnosis of melioidosis: an evaluation of a prototype immunochromatographic test. | to evaluate the panbio melioidosis prototype (ict) igg and igm test kits in detecting antibodies to burkholderia pseudomallei from an endemic region of australia. this would be of particular importance in high prevalence, low resource regions. | 2005 | 16028848 |
| changing epidemiology of melioidosis? a case of acute pulmonary melioidosis with fatal outcome imported from brazil. | melioidosis is an infectious disease caused by burkholderia pseudomallei. it is endemic in south east asia and tropical regions of northern australia. sporadic cases have been described elsewhere. in this article we present a case of acute pulmonary melioidosis with fatal outcome imported from brazil. the most common pathogen causing severe community-acquired pneumonia in brazil is streptococcus pneumoniae. other possible pathogens include legionella spp., mycoplasma pneumonia, gram-negative rod ... | 2005 | 16181507 |
| a case of melioidosis presenting as migrating pulmonary infiltration: the first case in korea. | melioidosis is an infection of the gram-negative bacterium burkholderia pseudomallei. while it is known as an important cause of sepsis or chronic abscess-forming disease in southeast asia and northern australia, no case has yet been reported in korea. a 50-yr-old man visited our hospital for intermittent fever associated with dry cough and sputum. roentgenographic examination showed migrating pulmonary infiltration. symptoms and chest radiograph and computed tomography (ct) image findings did n ... | 2005 | 15716619 |
| burkholderia pseudomallei invasion and activation of epithelial cells requires activation of p38 mitogen-activated protein kinase. | burkholderia pseudomallei is a causative agent of melioidosis. this gram-negative bacterium is able to survive inside the macrophages and also able to invade non-phagocytic cells including epithelial cells. interaction of pathogenic bacteria to the host cells is frequently associated with activation of mitogen-activated protein (map) kinases signaling activity. in this study, we demonstrated that b. pseudomallei stimulated p38 map kinase of human alveolar lung epithelial cell line (a549). phosph ... | 2005 | 15748812 |
| comparison of ashdown's medium, burkholderia cepacia medium, and burkholderia pseudomallei selective agar for clinical isolation of burkholderia pseudomallei. | ashdown's medium, burkholderia pseudomallei selective agar (bpsa), and a commercial burkholderia cepacia medium were compared for their abilities to grow b. pseudomallei from 155 clinical specimens that proved positive for this organism. the sensitivity of each was equivalent; the selectivity of bpsa was lower than that of ashdown's or b. cepacia medium. | 2005 | 16208018 |
| a molecular switch and electronic circuit modulate catalase activity in catalase-peroxidases. | the catalase reaction of catalase-peroxidases involves catalase-specific features built into a peroxidase core. an arginine, 20 a from the active-site heme, acts as a molecular switch moving between two conformations, one that activates heme oxidation and one that activates oxoferryl heme reduction by h(2)o(2), facilitating the catalatic pathway in a peroxidase. the influence of the arginine is imparted to the heme through its association with or dissociation from a tyrosinate that modulates rea ... | 2005 | 16211084 |
| pediatric melioidosis in pahang, malaysia. | melioidosis is much less common in children than in adults. this study investigated the incidence, demographic characteristics, presenting symptoms and outcome of pediatric melioidosis in pahang, malaysia. this retrospective study included patients < or =18 years old with positive body fluid cultures for burkholderia pseudomallei from january 2000 to june 2003. data on culture results were obtained from 2 referral hospitals. the incidence of pediatric melioidosis was 0.68/100,000 population per ... | 2005 | 16211138 |
| rapid identification of burkholderia pseudomallei in blood culture supernatants by a coagglutination assay. | in total, 309 blood culture supernatants were tested for the presence of burkholderia pseudomallei antigen using an in-house coagglutination test prepared by sensitising cowan i staphylococcal cells with b. pseudomallei polyclonal antiserum. the coagglutination test gave a sensitivity, specificity, positive predictive value and negative predictive value of 100% in comparison with blood culture. a subset of 102 supernatants was also tested for b. pseudomallei antigen using a monoclonal antibody-b ... | 2005 | 16216112 |
| time-kill curves as a tool for targeting ceftazidime serum concentration during continuous infusion for treatment of septicaemic melioidosis. | melioidosis is a fatal community-acquired infection endemic in tropical areas. ten isolates of the causative microorganism were subjected to time-kill study using a range of ceftazidime concentrations. this study demonstrated that a ceftazidime concentration of eight times the minimum inhibitory concentration yielded an optimal bactericidal effect and should be the target concentration administered by continuous infusion. | 2005 | 16216470 |
| relative uptake of technetium 99m stannous colloid by neutrophils and monocytes is altered by gram-negative infection. | gram-negative infection alters phagocytic cell function; hence, it could affect phagocytic uptake of inorganic colloids by these cells. neutrophil and monocyte uptake of technetium 99m stannous colloid (99mtc snc) in whole blood was measured in 10 patients with gram-negative infection (burkholderia pseudomallei) and 7 controls. mean uptake per individual neutrophil was reduced in infection. uptake per monocyte was not significantly different. blood from six normal individuals was incubated with ... | 2005 | 15691667 |
| brothers in arms. | 2005 | 15693175 | |
| melioidosis in 6 tsunami survivors in southern thailand. | six cases of melioidosis were identified in survivors of the 26 december 2004 tsunami who were admitted to takuapa general hospital in phangnga, a region in southern thailand where melioidosis is not endemic. all 6 cases were associated with aspiration, and 4 were also associated with laceration. | 2005 | 16142663 |
| antibodies from patients with melioidosis recognize burkholderia mallei but not burkholderia thailandensis antigens in the indirect hemagglutination assay. | the indirect hemagglutination assay routinely used to detect antibodies to burkholderia pseudomallei was modified to detect cross-reactivity of antibodies to b. pseudomallei, b. mallei, and b. thailandensis antigens. we demonstrate a lack of cross-reactivity between b. pseudomallei and b. thailandensis but marked cross-reactivity between b. pseudomallei and b. mallei. | 2005 | 16145163 |
| caspase-1 dependent macrophage death induced by burkholderia pseudomallei. | burkholderia pseudomallei is the causative agent for melioidosis, an infectious disease endemic in south-east asia and northern australia. infection can result in a wide spectrum of clinical outcomes, including asymtomatic, acute or chronic conditions. the ability of the bacteria to survive intracellularly within phagocytes and non-phagocytes is postulated to help this pathogen persist in the body during latent chronic conditions. in some gram-negative bacteria, such as shigella and salmonella, ... | 2005 | 16153244 |
| [new diagnostic methods for bacterial infections after the introduction of increased bioterrorism preparedness]. | bacillus anthracis, francicella tularensis, yersinia pestis, burkholderia pseudomallei and brucella melitensis are the bacteria most often mentioned in connection with possible biological terrorist acts. it is known that these microorganisms can be cultured and weaponised for such a purpose. this survey provides examples of the use of diagnostic methods for those microorganisms, further detailed in relation to establishing a national center for biological preparedness in denmark. | 2005 | 16159496 |
| infected pseudoaneurysm of the femoral artery secondary to melioidosis infection of a previous femoropopliteal bypass graft. | infected arterial aneurysm or pseudoaneurysm caused by burkholderia pseudomallei is very rare; a review of the literature reveals only five previously reported cases. we report the first case of melioidosis infection of a previous femoropopliteal bypass graft complicated by para-anastomotic pseudoaneurysm of the femoral artery successfully treated by surgical removal of the old graft and creation of a new, extraanatomic, external iliac-to-popliteal bypass graft. | 2005 | 15714374 |
| two randomized controlled trials of ceftazidime alone versus ceftazidime in combination with trimethoprim-sulfamethoxazole for the treatment of severe melioidosis. | two antibiotic regimens are used commonly in thailand for the initial treatment of severe melioidosis: ceftazidime in combination with trimethoprim-sulfamethoxazole (tmp-smx) and ceftazidime monotherapy. it is not known whether tmp-smx provides an additional benefit. | 2005 | 16163628 |
| etiologies and treatment outcomes in patients hospitalized with community-acquired pneumonia (cap) at srinagarind hospital, khon kaen, thailand. | local epidemiological data on the etiologies of in-patients who are hospitalized with cap is needed to develop guidelines for clinical practice. this study was conducted to determine the pattern of microorganisms causing community-acquired pneumonia (cap) in adult patients admitted to srinagarind hospital, khon kaen, thailand, between january 2001 and december 2002. altogether, 254 patients (124 males, 130 females) averaging 56.4 (sd 19.8) years were included. eighty-six of them (33.8%) presente ... | 2005 | 15906660 |
| pulmonary melioidosis associated with bronchiolitis obliterans organizing pneumonia. | we report a case of pulmonary melioidosis associated bronchiolitis obliterans with organizing pneumonia (boop). while antibiotic treatment alone did not show any major effect, addition of corticosteroids resulted in rapid clinical improvement. to our knowledge this is the first documented case of melioidosis associated with organising pneumonia. the possible therapeutic implications for the treatment of severe pulmonary melioidosis are discussed. | 2005 | 15603844 |
| integrative genomic, transcriptional, and proteomic diversity in natural isolates of the human pathogen burkholderia pseudomallei. | natural isolates of pathogenic bacteria can exhibit a broad range of phenotypic traits. to investigate the molecular mechanisms contributing to such phenotypic variability, we compared the genomes, transcriptomes, and proteomes of two natural isolates of the gram-negative bacterium burkholderia pseudomallei, the causative agent of the human disease melioidosis. significant intrinsic genomic, transcriptional, and proteomic variations were observed between the two strains involving genes of divers ... | 2005 | 15937190 |
| melioidosis: epidemiology, pathophysiology, and management. | melioidosis, caused by the gram-negative saprophyte burkholderia pseudomallei, is a disease of public health importance in southeast asia and northern australia that is associated with high case-fatality rates in animals and humans. it has the potential for epidemic spread to areas where it is not endemic, and sporadic case reports elsewhere in the world suggest that as-yet-unrecognized foci of infection may exist. environmental determinants of this infection, apart from a close association with ... | 2005 | 15831829 |
| epitope mapping of burkholderia pseudomallei serine metalloprotease: identification of serine protease epitope mimics. | filamentous phage random peptide libraries were used to identify the epitopes of burkholderia pseudomallei protease by panning against igg polyclonal sera that exhibited protease neutralizing properties. the isolated fusion peptides presented a consensus peptide sequence, tksmalsg, which closely resembles part of the active site sequence, 435gtsmatphvag445, of b. pseudomallei serine metalloprotease. by comparing the consensus sequence, tksmalsg, with the predicted three-dimensional molecular mod ... | 2005 | 15607634 |
| route of infection in melioidosis. | 2005 | 15834987 | |
| detection of paragonimus heterotremus eggs in experimentally infected cats by a polymerase chain reaction-based method. | a polymerase chain reaction (pcr) procedure for the detection of paragonimus heterotremus eggs in stool samples was developed and compared with stoll's egg count method. the primers were designed on the basis of a previously constructed pph-13-specific dna probe, which produced an approximate 0.5-kb amplified product. this pcr method could detect as few as 5 eggs in 0.6 g of artificially inoculated feces of a healthy control cat or as little as 1 x 10(-4) ng of p. heterotremus genomic dna. the a ... | 2005 | 15856902 |
| musculoskeletal melioidosis masquerading as diabetic amyotrophy. | a patient with musculoskeletal melioidosis masquerading as diabetic amyotrophy is described. a 43-year-old man presented with left thigh pain, fever, malaise and loss of weight. he had diabetes mellitus for six years. he was initially diagnosed with diabetic amyotrophy and was treated conservatively. recurrence of symptoms prompted further investigations which revealed melioidosis of the left femur. magnetic resonance imaging showed an enhancing subperiosteal collection. the diagnosis was confir ... | 2005 | 15858693 |
| comparison of diagnostic laboratory methods for identification of burkholderia pseudomallei. | limited experience and a lack of validated diagnostic reagents make burkholderia pseudomallei, the cause of melioidosis, difficult to recognize in the diagnostic microbiology laboratory. we compared three methods of confirming the identity of presumptive b. pseudomallei strains using a collection of burkholderia species drawn from diverse geographic, clinical, and environmental sources. the 95 isolates studied included 71 b. pseudomallei and 3 b. thailandensis isolates. the api 20ne method ident ... | 2005 | 15872242 |
| role and significance of quantitative urine cultures in diagnosis of melioidosis. | melioidosis is associated with significant mortality in countries in which it is endemic. previous studies have demonstrated that quantitative burkholderia pseudomallei counts in blood are predictive of mortality. here we examine the relationship between outcomes and quantitative b. pseudomallei counts in urine. a total of 755 patients presenting to sappasithiprasong hospital, ubon ratchathani, northeast thailand (in the northeast part of the country), with melioidosis between july 1993 and octo ... | 2005 | 15872255 |
| the role and significance of sputum cultures in the diagnosis of melioidosis. | pneumonia is a common manifestation of melioidosis, the disease caused by burkholderia pseudomallei. in this study, we defined the prognostic significance of a positive sputum culture. a total of 712 patients presenting to sappasithiprasong hospital, ubon ratchathani, thailand, with melioidosis between january 1992 and december 2002 had a sputum culture performed during admission, which was positive for b. pseudomallei in 444 patients (62%). the median duration of sputum positivity was 9 days (r ... | 2005 | 16222004 |
| melioidosis and the vascular surgeon: hospital kuala lumpur experience. | bacterial arteritis is relatively uncommon and management of this condition, which carries high morbidity and mortality, is difficult and time-consuming. common organisms implicated include salmonella and staphylococcus. arteritis as a result of infection by burkholderia pseudomallei (formerly pseudomonas pseudomallei) has been rarely reported in the english literature. this organism, which is endemic in our part of the world, is well known to cause a wide spectrum of septic conditions. a review ... | 2005 | 16234087 |
| burkholderia pseudomallei-induced expression of suppressor of cytokine signaling 3 and cytokine-inducible src homology 2-containing protein in mouse macrophages: a possible mechanism for suppression of the response to gamma interferon stimulation. | burkholderia pseudomallei, the causative agent of melioidosis, is a facultative intracellular gram-negative bacterium that is able to survive and multiply in macrophages. previously, we reported that b. pseudomallei was able to escape macrophage killing by interfering with the expression of inducible nitric oxide synthase (inos). in the present study, we extended this finding and demonstrated that b. pseudomallei was able to activate the expression of suppressor of cytokine signaling 3 (socs3) a ... | 2005 | 16239531 |
| genome-wide expression analysis of iron regulation in burkholderia pseudomallei and burkholderia mallei using dna microarrays. | burkholderia pseudomallei and b. mallei are the causative agents of melioidosis and glanders, respectively. as iron regulation of gene expression is common in bacteria, in the present studies, we have used microarray analysis to examine the effects of growth in different iron concentrations on the regulation of gene expression in b. pseudomallei and b. mallei. gene expression profiles for these two bacterial species were similar under high and low iron growth conditions irrespective of growth ph ... | 2005 | 16242861 |
| serodiagnosis of melioidosis by a competitive enzyme-linked immunosorbent assay using a lipopolysaccharide-specific monoclonal antibody. | burkholderia pseudomallei is the causative agent of melioidosis, a severe and potentially fatal infectious disease in humans known to be endemic in southeast asia and northern australia. the infection is also increasingly recognized in various animal species with a potential to spread to humans. with the potential as a biological warfare agent, specific serodiagnosis of melioidosis for surveillance in large populations at risk, humans or animals, would be highly valuable. in this study, a compet ... | 2005 | 16252843 |
| in disaster's wake: tsunami lung. | 2005 | 16263492 | |
| humoral and cell-mediated adaptive immune responses are required for protection against burkholderia pseudomallei challenge and bacterial clearance postinfection. | burkholderia pseudomallei, the causative agent of melioidosis, is a gram-negative bacillus endemic to areas of southeast asia and northern australia. presently, there is no licensed vaccine for b. pseudomallei and the organism is refractive to antibiotic therapy. the bacterium is known to survive and multiply inside both phagocytic and nonphagocytic host cells and may be able to spread directly from cell to cell. current vaccine delivery systems are unlikely to induce the correct immune effector ... | 2005 | 16113315 |
| seroprevalence of melioidosis in diabetic patients in taiwan. | diabetes mellitus is a major predisposing factor for burkholderia pseudomallei infection. this study surveyed serum samples from 356 taiwanese patients with diabetes mellitus for anti-flagellin antibodies against b. pseudomallei. antibody titer to b. pseudomallei was positive in 3.0% (11/365) of diabetes mellitus patients. all seropositive individuals were aged > or =60, indicating that elderly and diabetic adults are at high risk of b. pseudomallei infection. in this study, diabetic females, wh ... | 2005 | 16118674 |
| multinucleated giant cell formation and apoptosis in infected host cells is mediated by burkholderia pseudomallei type iii secretion protein bipb. | 2005 | 16159789 | |
| melioidosis: the johor bahru experience. | a 5 year retrospective review of cases of melioidosis was carried out in sultanah aminah hospital, johor bahru. there were 44 new cases of melioidosis which was proven by either blood or pus culture growing burkholderia pseudomallei from the period between january 1999 and december 2003. of these, 38 (86.4%) were males compared to only 6 (13.6%) females. thirty-one (70.5%) were malays, 7 (15.9%) were chinese, 5 (11.4%) were indians and 1 (2.2%) was a sarawakian. the peak age group was between 50 ... | 2005 | 16515111 |
| the burkholderia pseudomallei rpoe (algu) operon is involved in environmental stress tolerance and biofilm formation. | burkholderia pseudomallei, the causative agent of melioidosis, can be isolated from soil and water. to persist, adapt and survive within and outside their human host, bacteria rely on regulatory mechanisms that allow them to respond rapidly to stressful situations. we have examined the possible role of b. pseudomallei alternative sigma factor sigma(e) (rpoe) in the stress response and found that rpoe and its putative regulators (bpre-rseb-mucd) are transcribed in a single transcriptional unit. i ... | 2005 | 16185818 |
| open-label randomized trial of oral trimethoprim-sulfamethoxazole, doxycycline, and chloramphenicol compared with trimethoprim-sulfamethoxazole and doxycycline for maintenance therapy of melioidosis. | melioidosis (infection caused by burkholderia pseudomallei) requires a prolonged course of oral antibiotics following initial intravenous therapy to reduce the risk of relapse after cessation of treatment. the current recommendation is a four-drug regimen (trimethoprim [tmp], sulfamethoxazole [smx], doxycycline, and chloramphenicol) and a total treatment time of 12 to 20 weeks. drug side effects are common; the aim of this study was to compare the efficacy and tolerance of the four-drug regimen ... | 2005 | 16189075 |
| seroprevalence of burkholderia pseudomallei in east timorese refugees: implications for healthcare in east timor. | melioidosis is a disease with protean clinical manifestations caused by the bacterium burkholderia pseudomallei. it is endemic in countries surrounding the newly independent east timor, but has yet to be isolated or demonstrated serologically in that country. one illness that can be clinically indistinguishable from melioidosis is pulmonary tuberculosis, a condition with a very high prevalence in east timor. we used an indirect hemagglutination test (iha) to measure antibodies to b. pseudomallei ... | 2005 | 16610652 |
| [properties of the burkholderia pseudomallei insertional mutants deficient in membrane proteins production]. | transposon-induced b. pseudomallei mutants deficient in membrane proteins production were obtained for evaluation of the functional role of these cell components. in comparison with the wild type strain b. pseudomallei 57576, mutant clones ttm6, ttm7 and ttm9 carrying tn5 chromosome insertions were characterized by lost or decreased production of outer membrane proteins 27, 48, 52, 150, 200 kda. alterations in outer membrane protein spectra were accompanied by twofold increase in susceptibility ... | 2005 | 16334220 |
| short report: disease severity and outcome of melioidosis in hiv coinfected individuals. | this study examined whether coinfection with hiv and burkholderia pseudomallei leads to altered disease severity or outcome associated with melioidosis. coinfection was detected in only 8 of 524 (1.5%) adults with melioidosis in northeast thailand. clinical presentation and acute outcome were similar in hiv-positive and hiv-negative patients. | 2005 | 16354832 |
| the identification and characterization of two phosphatidylinositol-4,5-bisphosphate 4-phosphatases. | numerous inositol polyphosphate 5-phosphatases catalyze the degradation of phosphatidylinositol-4,5-bisphosphate (ptdins-4,5-p(2)) to phosphatidylinositol-4-phosphate (ptdins-4-p). an alternative pathway to degrade ptdins-4,5-p(2) is the hydrolysis of ptdins-4,5-p(2) by a 4-phosphatase, leading to the production of ptdins-5-p. whereas the bacterial ipgd enzyme is known to catalyze this reaction, no such mammalian enzyme has been found. we have identified and characterized two previously undescri ... | 2005 | 16365287 |
| the effect of methanolic extract of tamarindus indica linn. on the growth of clinical isolates of burkholderia pseudomallei. | burkholderia pseudomallei (pseudomonas pseudomallei) causes melioidosis, a life-threatening infection common among paddy cultivators in southeast asian countries. no plant materials have been investigated for its activity against b. pseudomallei. therefore, a preliminary study was carried out using disc diffusion and minimum inhibitory concentration (mic) methods to evaluate the anti-b. pseudomallei activity of five indian medicinal plants documented to have been used for several ailments in the ... | 2005 | 16518004 |
| attempted passive prophylaxis with a monoclonal anti-burkholderia pseudomallei exopolysaccharide antibody in a murine model of melioidosis. | melioidosis is a severe gram-negative infection caused by the facultative intracellular bacterium burkholderia pseudomallei, which is responsible for a broad spectrum of symptoms in both humans and animals. no licensed vaccine currently exists. this study evaluated the protective effect of a monoclonal antibody (mab ps6f6) specific to b. pseudomallei exopolysaccharide in an outbred murine model of sub-acute melioidosis. when administered before the infectious challenge, ps6f6 significantly incre ... | 2005 | 16435577 |
| short report: application of a polymerase chain reaction to detect burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis. | the diagnostic potential of a burkholderia pseudomallei type three secretion system (tts1) polymerase chain reaction (pcr) was examined on clinical specimens from 27 patients with sepsis in the northern territory of australia, a region endemic for melioidosis. the tts1 pcr was conducted on dna extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). the pcr sensitivity in culture-positive cli ... | 2005 | 16354831 |
| an electronmicroscopic study of the interaction of burkholderia pseudomallei and human macrophages. | b. pseudomallei has been shown to persist intracellularly in melioidosis patients until reactivated by decreasing immunocompetence. we have shown by transmission electron microscopy the internalization of b. pseudomallei by human macrophages via conventional phagocytosis enclosed within membrane-bound vacuoles or phagosomes. ferritin labeled lysosomes provided evidence of phagosome-lysosome fusion. ingested bacilli were designated as "intact" or "damaged" on the basis of their ultrastructural fe ... | 2005 | 16676686 |
| burkholderia pseudomallei infections in finnish tourists injured by the december 2004 tsunami in thailand. | 2005 | 16702634 | |
| identification of burkholderia pseudomallei genes required for the intracellular life cycle and in vivo virulence. | the bacterial pathogen burkholderia pseudomallei invades host cells, escapes from endocytic vesicles, multiplies intracellularly, and induces the formation of actin tails and membrane protrusions, leading to direct cell-to-cell spreading. this study was aimed at the identification of b. pseudomallei genes responsible for the different steps of this intracellular life cycle. b. pseudomallei transposon mutants were screened for a reduced ability to form plaques on ptk2 cell monolayers as a result ... | 2006 | 16714590 |
| genomic patterns of pathogen evolution revealed by comparison of burkholderia pseudomallei, the causative agent of melioidosis, to avirulent burkholderia thailandensis. | the gram-negative bacterium burkholderia pseudomallei (bp) is the causative agent of the human disease melioidosis. to understand the evolutionary mechanisms contributing to bp virulence, we performed a comparative genomic analysis of bp k96243 and b. thailandensis (bt) e264, a closely related but avirulent relative. | 2006 | 16725056 |
| management of melioidosis. | melioidosis is a serious human infection caused by the environmental gram-negative bacterium burkholderia pseudomallei. outcome following melioidosis remains poor despite 20 years of clinical research. overall mortality is 50% in north-east thailand (35% in children) and 19% in australia. relapse is common (13% over 10 years), and results from failure to eradicate the organism. treatment is required to complete 12-20 weeks, or longer if clinically indicated. this is divided into intravenous and ... | 2006 | 16771621 |
| quorum sensing regulates dpsa and the oxidative stress response in burkholderia pseudomallei. | burkholderia pseudomallei is the causative agent of melioidosis, a fatal human tropical disease. the non-specific dna-binding protein dpsa plays a key role in protecting b. pseudomallei from oxidative stress mediated, for example, by organic hydroperoxides. the regulation of dpsa expression is poorly understood but one possibility is that it is regulated in a cell population density-dependent manner via n-acylhomoserine lactone (ahl)-dependent quorum sensing (qs) since a lux-box motif has been l ... | 2006 | 17159218 |
| unrecognised infection in a cystic fibrosis patient. | we report a 17-year-old malay boy with cystic fibrosis who over a 14-month period experienced worsening respiratory symptoms and deteriorating lung function. burkholderia pseudomallei was eventually isolated from his sputum. he improved clinically following treatment for meliodosis and his lung function returned to normal. | 2006 | 16630326 |
| fatal burkholderia pseudomallei septicaemia. | 2006 | 17180814 | |
| functional genetic analysis reveals a 2-alkyl-4-quinolone signaling system in the human pathogen burkholderia pseudomallei and related bacteria. | pseudomonas aeruginosa synthesizes diverse 2-alkyl-4(1h)-quinolones (ahqs), including the signaling molecule 2-heptyl-3-hydroxy-4(1h)-quinolone (pqs), via the pqsabcde locus. by examining the genome databases, homologs of the pqs genes were identified in other bacteria. however, apart from p. aeruginosa, only burkholderia pseudomallei and b. thailandensis contained a complete pqsa-e operon (termed hhqa-e). by introducing the b. pseudomallei hhqa and hhqe genes into p. aeruginosa pqsa and pqse mu ... | 2006 | 16873018 |
| multilocus sequence typing of historical burkholderia pseudomallei isolates collected in southeast asia from 1964 to 1967 provides insight into the epidemiology of melioidosis. | a collection of 207 historically relevant burkholderia pseudomallei isolates was analyzed by multilocus sequence typing (mlst). the strain collection contains environmental isolates obtained from a geographical distribution survey of b. pseudomallei isolates in thailand (1964 to 1967), as well as stock cultures and colony variants from the u.s. army medical research unit (malaysia), the walter reed army institute for research, and the pasteur institute (vietnam). the 207 isolates of the collecti ... | 2006 | 16891516 |
| clinical evaluation of a type iii secretion system real-time pcr assay for diagnosing melioidosis. | a burkholderia pseudomallei type iii secretion system real-time pcr assay was evaluated on clinical specimens in a region where melioidosis is endemic. the pcr was positive in 30/33 (91%) patients with culture-confirmed melioidosis. all six patients with melioidosis septic shock were blood pcr positive, suggesting potential for rapid diagnosis and commencement of appropriate therapy. | 2006 | 16891534 |
| temperature-regulated microcolony formation by burkholderia pseudomallei requires pila and enhances association with cultured human cells. | burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease that is endemic to northern australia and southeast asia and is acquired from soil or water. adherence of b. pseudomallei 08 to cultured cells increases dramatically following prior growth at 30 degrees c or less compared to that following prior growth at 37 degrees c. here, we show that this occurs almost entirely as the result of microcolony formation (bacterium-bacterium interactions) following growth ... | 2006 | 16926432 |
| the innate interferon gamma response of balb/c and c57bl/6 mice to in vitro burkholderia pseudomallei infection. | burkholderia pseudomallei is the causative agent for melioidosis. for many bacterial infections, cytokine dysregulation is one of the contributing factors to the severe clinical outcomes in the susceptible hosts. the c57bl/6 and balb/c mice have been established as a differential model of susceptibility in murine melioidosis. in this study, we compared the innate ifn-gamma response to b. pseudomallei between the c57bl/6 and balb/c splenocytes and characterized the hyperproduction of ifn-gamma in ... | 2006 | 16919160 |
| in silico analysis of burkholderia pseudomallei genome sequence for potential drug targets. | recent advances in dna sequencing technology have enabled elucidation of whole genome information from a plethora of organisms. in parallel with this technology, various bioinformatics tools have driven the comparative analysis of the genome sequences between species and within isolates. while drawing meaningful conclusions from a large amount of raw material, computer-aided identification of suitable targets for further experimental analysis and characterization, has also led to the prediction ... | 2006 | 16922696 |
| development of mouse hybridomas for production of monoclonal antibodies specific to burkholderia mallei and burkholderia pseudomallei. | burkholderia mallei and b. pseudomallei are designated category b biothreat agents on the "select agents" list established by the nih and cdc. development of monoclonal antibodies (mabs) that could effectively differentiate these two closely related species of bacteria and other non-pathogenic burkholderia bacteria is urgently needed. splenocytes from mice immunized with various antigen preparations from either b. mallei (american type culture collection [atcc] 23344) or b. pseudomallei (atcc 23 ... | 2006 | 16934015 |
| pyogenic liver abscess caused by burkholderia pseudomallei in taiwan. | pyogenic liver abscess in taiwan is a well-known disease entity, commonly associated with a single pathogen, klebsiella pneumoniae. melioidosis is an endemic disease in taiwan that can manifest as multiple abscesses in sites including the liver. we report three cases of liver abscesses caused by burkholderia pseudomallei. the first patient was a 54-year-old diabetic woman, who presented with liver abscess and a left subphrenic abscess resulting from a ruptured splenic abscess, co-infected with k ... | 2006 | 16935773 |
| mycobacterium avium and burkholderia pseudomallei (melioidosis) coinfection in an hiv-positive patient. | a 29 year old hiv positive thai female with cd4 count of 10 cells/mm3 presented with chronic diffuse abdominal pain, fever, weight loss, anemia and leucopenia. ultrasonography demonstrated diffuse upper abdominal lymphadenopathy with ascites. microbiological and molecular work up of the specimen obtained by ultrasound-guided lymph node aspiration revealed co-infection with burkholderia pseudomallei and mycobacterium avium. indirect hemagglutination, igm-indirect fluorescent antibody, and igg-ind ... | 2006 | 17348247 |
| antibiotic susceptibility of 50 clinical isolates of burkholderia pseudomallei from singapore. | 2006 | 16951412 | |
| burkholderia oklahomensis sp. nov., a burkholderia pseudomallei-like species formerly known as the oklahoma strain of pseudomonas pseudomallei. | c6786, the clinical isolate of the 'oklahoma' strain of pseudomonas (now burkholderia) pseudomallei, was originally isolated in 1973 from a wound infection resulting from a farming accident in oklahoma, usa. environmental isolates c7532 and c7533 from the oklahoma accident site were found to match c6786. these three isolates and a clinical isolate originally identified as b. pseudomallei that was recovered from a person in georgia, usa, involved in an automobile accident were characterized by bi ... | 2006 | 16957116 |
| melioidosis: a potentially life threatening infection. | melioidosis is caused by the gram-negative bacillus, burkholderia pseudomallei. it is endemic in tropical australia and in southeast asian countries. the overall mortality from this infection remains extremely high despite recent advancement in its treatment. this review discuss about clinical manifestations, diagnosis and management of melioidosis. | 2006 | 17240600 |
| the mvin homolog in burkholderia pseudomallei is essential for viability and virulence. | the virulence factors of burkholderia pseudomallei, the causative agent of melioidosis, are not fully understood. we have identified a gene with homology to the salmonella typhimurium mouse virulence gene, mvin, a member of the mouse virulence factor family. expression studies with an insertional mutant containing a lux operon demonstrated that the expression of the gene is influenced by free-iron availability in the media and by growth phase. the mutant displayed an increased ld50 value in the ... | 2006 | 17110975 |
| short report: melioidosis in myanmar: forgotten but not gone? | a serologic survey of adults resident in myanmar was conducted to define the presence of antibodies to burkholderia pseudomallei, the cause of melioidosis. antibodies were detectable by indirect hemagglutination assay (iha) in 757 (78%) of 968 adults, of whom 69 (7%) had an iha titer > or =1:160. | 2006 | 17123993 |
| genome-wide expression analysis of burkholderia pseudomallei infection in a hamster model of acute melioidosis. | burkholderia pseudomallei is the causative agent of melioidosis and represents a potential bioterrorism threat. in the current studies we have examined gene expression in b. pseudomallei in an animal model of acute melioidosis using whole-genome microarrays. gene expression profiles were generated by comparing transcriptional levels of b. pseudomallei-expressed genes in infected hamster organs including liver, lung, and spleen following intraperitoneal and intranasal routes of infection to those ... | 2006 | 16988221 |
| pneumonia and septicemia caused by burkholderia thailandensis in the united states. | burkholderia thailandensis is closely related to burkholderia pseudomallei, the causative agent of melioidosis. it is generally considered avirulent and previously has been reported to occur only in southeast asia. we report the first case of pneumonia and septicemia caused by b. thailandensis in the united states. | 2006 | 17050819 |
| pcr-based identification of burkholderia pseudomallei. | dna amplification techniques are being used increasingly in clinical laboratories to confirm the identity of medically important bacteria. a pcr-based identification method has been in use in our centre for 10 years for burkholderia pseudomallei and was used to confirm the identity of bacteria isolated from cases of melioidosis in ceará since 2003. this particular method has been used as a reference standard for less discriminatory methods. in this study we evaluated three pcr-based methods of b ... | 2006 | 17086309 |