Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| effects of porphyrins and inorganic iron on the growth of prevotella intermedia. | we demonstrated earlier that hemin-iron-containing compounds which include hemin, human hemoglobin, bovine hemoglobin, and bovine catalase stimulate the growth of prevotella intermedia [leung, subramaniam, okamoto, fukushima, lai, fems microbiol. lett. 162 (1998) 227-233]. however, the contributions of tetrapyrrole porphyrin ring in these hemin-iron sources as well as inorganic iron for the growth of this organism have not been determined. the purpose of this study was to examine the effects of ... | 2002 | 12007648 |
| dipeptidyl peptidase with strict substrate specificity of an anaerobic periodontopathogen porphyromonas gingivalis. | a dipeptidyl peptidase which hydrolyzed xaa-ala-p-nitroanilide was purified to homogeneity by sequential procedures including ammonium sulfate precipitation, ion-exchange chromatography, hydrophobic interaction chromatography, gel filtration and isoelectric focusing from the cell extract of porphyromonas gingivalis. the purified enzyme hydrolyzed p-nitroanilide derivatives of lys-ala, ala-ala, and val-ala, but not xaa-pro. enzyme activity was maximum at neutral phs. its molecular mass was 64 kda ... | 2002 | 12007665 |
| serum igg antibody response to periodontal pathogens in minority populations: relationship to periodontal disease status and progression. | differences in periodontal disease prevalence, severity, subgingival microflora and host immune response have been reported for various ethnic/racial groups, which implies that risk factors for destructive periodontal disease progression may also vary in these populations. as it is possible that these differences may be due to confounding variables other than ethnicity/race, we have measured serum igg antibody response to six periodontal pathogens, and compared these data with microbiological, c ... | 2002 | 12009183 |
| parotid salivary s-iga antibodies during experimental gingivitis in smokers and non-smokers. | persons who smoke display a less pronounced increase of gingival bleeding in the experimental gingivitis model as compared with non-smokers. the aim of the present study was to investigate whether this could partly be explained by differences in levels of parotid total secretory iga (s-iga) or parotid s-iga reactive with selected oral microorganisms. parotid saliva samples were obtained from 11 smoking and 14 non-smoking volunteers, at baseline, after 5 and 14 days of full mouth experimental gin ... | 2002 | 12009188 |
| mice lacking monocyte chemoattractant protein 1 have enhanced susceptibility to an interstitial polymicrobial infection due to impaired monocyte recruitment. | monocyte chemoattractant protein 1 (mcp-1) is an important chemokine that induces monocyte recruitment in a number of different pathologies, including infection. to investigate the role of mcp-1 in protecting a host from a chronic interstitial polymicrobial infection, dental pulps of mcp-1(-/-) mice and controls were inoculated with six different oral pathogens. in this model the recruitment of leukocytes and the impact of a genetic deletion on the susceptibility to infection can be accurately a ... | 2002 | 12011011 |
| topical antiseptics and antibiotics in the initial therapy of chronic adult periodontitis: microbiological aspects. | 2002 | 12013349 | |
| interleukin-10 inhibits expression of interleukin-6 and -8 mrna in human dental pulp cell cultures via nuclear factor-kappab deactivation. | the effects of interleukin (il)-10 on the expression of il-6 and il-8 mrna in human dental pulp cell cultures were investigated by using the northern blot analysis. on stimulation with prevotella intermedia lipopolysaccharide (pilps), il-10 was produced in peripheral blood but was not detected in human dental pulp cell culture supernatants. il-10 inhibited il-8 mrna expression, which is normally stimulated by pilps, il-1alpha, and tumor necrosis factor-alpha and inhibited il-6 mrna expression, w ... | 2002 | 12017175 |
| combinations of bacterial species in endodontic infections. | this study was undertaken to investigate combinations of bacteria found in root-canal infections of teeth with periapical bone destruction without clinical signs and symptoms. | 2002 | 12196223 |
| microbiological, immunological and genetic factors in family members with periodontitis as a manifestation of systemic disease, associated with hematological disorders. | the microflora, immunological profiles of host defence functions, and human leukocyte antigen (hla) findings are reported for a mother, son and daughter who were diagnosed as having 'periodontitis as a manifestation of systemic diseases, associated with hematological disorders'. examinations were made of the bacterial flora from the periodontal pocket, neutrophil chemotaxis, neutrophil phagocytosis, and the genotypes (dqb1) and serotypes (dr locus) of hla class ii antigens. phenotypic analyses o ... | 2002 | 12200976 |
| prevotella intermedia native plasmid can be mobilized by an escherichia coli conjugal incp plasmid. | we have determined the nucleotide sequence of a small prevotella intermedia cryptic plasmid, pyhbi1, which consisted of sequences that were highly homologous to the amino acid sequence of the replication and mobilization proteins found in related organisms. we have also demonstrated that chimeric plasmids derived from this p. intermedia native plasmid can be mobilized between escherichia coli strains by using a broad-host-range e. coli conjugative plasmid, incp plasmid rp4. the results suggest t ... | 2002 | 12206757 |
| frequency of reactivity for porphyromonas gingivalis and prevotella spp. in supra- and subgingival plaques, and periodontal clinical parameters according to subject age. | the present study was conducted to assess the association between selected clinical parameters and the distribution of porphyromonas gingivalis (pg), prevotella intermedia (pi), prevotella nigrescens (pn), and prevotella melaninogenica (pm) in supra- and subgingival plaque samples measured by an immunoslot blot assay (iba) using their monoclonal antibodies. | 2002 | 12211497 |
| gtr treatment of intrabony defects in patients with early-onset and chronic adult periodontitis. | young, systemically healthy subjects may suffer from early-onset forms of periodontitis characterized by the presence of localized deep vertical bony defects. the aim of this study was to compare the healing response after guided tissue regeneration (gtr) treatment of similar intrabony defects in patients affected by early-onset and chronic adult periodontitis. twenty systemically healthy, nonsmoking subjects were enrolled in the study; 10 were affected by early-onset periodontitis (eop) and 10 ... | 2002 | 12212679 |
| [an in vitro study of the action of kola nitida on bacterial strains implicated in dental caries and periodontal diseases]. | the nitida kola is a substance extracted from the kolanut. in west africa its use by chewing is widespread among the manding people. it's said to have tonic, stimulant and aphrodisiac characteristics and even recent studies have shown that it has antibacterial characteristics. the aim of this study is to make an estimation of the nitida kola's effects on different bacterial species involved in the two main oral and dental pathologies (teeth decays and periodontal illnesses). the obtained results ... | 2002 | 12221807 |
| biochemical-genetic analysis and distribution of des-1, an ambler class a extended-spectrum beta-lactamase from desulfovibrio desulfuricans. | desulfovibrio spp. are gram-negative anaerobes phylogenetically related to bacteroides spp., which are rarely isolated and which are mostly isolated from intra-abdominal abscesses. desulfovibrio desulfuricans clinical isolate d3 had a clavulanic acid-inhibited beta-lactam resistance profile and was resistant to some expanded-spectrum cephalosporins. a beta-lactamase gene, bla(des-1), was cloned from whole-cell dna of isolate d3 and expressed in escherichia coli. purified beta-lactamase des-1, wi ... | 2002 | 12234847 |
| relationship of changes in interleukin-8 levels and granulocyte elastase activity in gingival crevicular fluid to subgingival periodontopathogens following non-surgical periodontal therapy in subjects with chronic periodontitis. | to determine the effect of scaling and root planing (srp) on the interrelations of subgingival periodontopathogens and both interleukin-8 (il-8) and granulocyte elastase activity in gingival crevicular fluid (gcf), and to assess their relations to the short-term treatment response in management of chronic periodontitis. | 2002 | 12354085 |
| comparison of microbial cultivation and a commercial pcr based method for detection of periodontopathogenic species in subgingival plaque samples. | microbiological laboratory procedures are involved in diagnosis and therapy control of progressive and refractory forms of periodontitis. in recent years techniques have been developed based on the detection of nucleic acids. the purpose of this study was to validate the commercially available micro-dent(r) test which employs probes for a. actinomycetemcomitans, p. gingivalis, p. intermedia, b. forsythus and t. denticola. | 2002 | 12354089 |
| initial effect of controlled release chlorhexidine on subgingival microorganisms. | little or no data exist on the ability of subgingival application of periochip (2.5 mg chlorhexidine gluconate in a biodegradable chip; astra pharmaceuticals, westborough, ma, usa) to suppress periodontopathic microorganisms. the present study compared the subgingival microbiota of periodontitis sites receiving the chlorhexidine chip plus scaling and root planing (sc/rp) or sc/rp alone. | 2002 | 12366861 |
| transmission of periodontal disease-associated bacteria from teeth to osseointegrated implant regions. | the presence of periodontopathic bacteria is a risk factor for peri-implantitis. the present study examined colonization by periodontopathic bacteria and their transmission from periodontal pockets to osseointegrated implant sulcus. | 2002 | 12381070 |
| oro-dental bacteria in various atherosclerotic arteries. | chlamydia pneumoniae dna has been detected in at least 40% of all major arteries affected by atherosclerosis, but several other microorganisms have also been detected. in this study, diseased vessels were evaluated for the presence of the dna of seven oro-dental bacteria and two nonoral bacteria. a polymerase chain reaction technique was employed using primer pairs based on 16s rrna genes. of 32 specimens tested, 10 (31.2%) were dna positive: seven for actinobacillus actinomycetemcomitans and th ... | 2002 | 12415477 |
| extract and identify ingredient from ligustrum lucidum ait and study its effect to periodontal pathogen. | to extract the effective ingredient (crystal i) from effective section (saponin) of ligustrum lucidum ait, identify the chemical structure of crystal i, study the effect of crystal i on p. gingivalis, b. forsythus and p. intermedia. | 2002 | 12425858 |
| presence and antibiotic resistance of porphyromonas gingivalis, prevotella intermedia, and prevotella nigrescens in children. | only limited information exists about the prevalence in children of pathogens associated with periodontitis. the aim of the present study was to determine by culture whether 8-11-year-old children carry porphyromonas gingivalis, prevotella intermedia, and/or p. nigrescens in samples from the gingiva and/or the buccal mucosa taken before, and after caries treatment and oral hygiene instruction. a second aim was to assess the proportion of subjects who had gram-negative anaerobes carrying the tet( ... | 2002 | 12445225 |
| effects of lipopolysaccharide extracted from prevotella intermedia on bone formation and on the release of osteolytic mediators by fetal mouse osteoblasts in vitro. | prevotella intermedia, a gram-negative obligate anaerobic black-pigmented oral bacterium, belongs to a small group of microorganisms that is closely associated with the initiation of periodontal diseases. lipopolysaccharide (lps), an outer membrane component, is one of the main virulence factors of this bacterium. the aim of this study was to examine the effects of prev. intermedia lipopolysaccharide, extracted by the hot-phenol-water method, on differentiation (alkaline phosphatase activity) an ... | 2002 | 12450517 |
| microbial susceptibility to calcium hydroxide pastes and their vehicles. | the aim of this study was to investigate the susceptibility of some microorganisms commonly isolated from root canals to calcium hydroxide in combination with several vehicles by the agar diffusion method. stainless-steel cylinders were placed on each inoculated agar medium. the test medications and their controls were placed inside the cylinders. the zones of growth inhibition were measured and recorded after the incubation period for each plate, and the results were analyzed statistically. ent ... | 2002 | 12470019 |
| porphyromonas gingivalis, bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction. | bacteria play an essential role in the pathogenesis of destructive periodontal disease. it has been suggested that not all bacteria associated with periodontitis may be normal inhabitants of a periodontally healthy dentition. in particular, porphyromonas gingivalis and actinobacillus actinomycetemcomitans have been isolated infrequently from subjects without periodontitis. the aim of the present study was to compare prevalence and proportions of a number of periodontal bacteria in periodontitis ... | 2002 | 12472995 |
| in vitro effect of laser irradiation on cementum-bound endotoxin isolated from periodontally diseased roots. | in a previous study, we evaluated the in vivo effects of an nd:yag laser on periodontal disease by measuring crevicular interleukin (il)-1beta levels before and after laser application. it was found that laser therapy was less effective than traditional scaling and root planing. these results might be due to incomplete removal of microbial residues and cementum-bound endotoxin on root surfaces by the laser. in this study, we explored the in vitro effectiveness of an nd:yag laser for the eliminat ... | 2002 | 12479628 |
| correlations between bacterial levels in autologous subgingival plaque and saliva of adult sudanese. | the aim of this study was to assess levels of oral bacteria and their correlations in paired samples of saliva and subgingival plaque in a population of adult sudanese. whole saliva and pooled subgingival plaque samples from six probing sites of one tooth in each jaw were obtained from 56 sudanese adults (mean age 35.2+/-8.9 years). levels of 24 oral bacteria in the autologous saliva and pooled plaque sample of each subject were assessed by dna probes and checkerboard dna-dna hybridization. ther ... | 2002 | 12483235 |
| similarity of the oral microbiota of pre-school children with that of their caregivers in a population-based study. | this study evaluated the similarity between the oral microbiota of young children and that of their adult caregivers. oral samples from children (174 dentate and 18 pre-dentate) aged 6-36 months and their caregivers in saipan were assayed using a dna probe assay. many species including streptococcus mutans, streptococcus sobrinus, actinomyces species, campylobacter rectus, fusobacterium nucleatum, prevotella intermedia, and porphyromonas gingivalis were detected in dentate and pre-dentate childr ... | 2002 | 12485330 |
| microbiota around root-form endosseous implants: a review of the literature. | although high success rates for root-form endosseous implants have been reported, failures occasionally occur, and these implants must be removed. at least 10% of the failures have been suggested to be the result of peri-implantitis. there is some evidence that periodontal pathogens, mainly those belonging to the group of gram-negative anaerobic rods, play a role in the etiology of peri-implantitis. this article provides an overview of the literature associated with common peri-implant microbiol ... | 2002 | 12507243 |
| periodontopathic bacterial infection in childhood. | little information is available on periodontopathic bacterial infection in childhood. we assessed the prevalence by age of 10 putative periodontopathic microorganisms in periodontally healthy children using a polymerase chain reaction (pcr) assay. | 2002 | 11846195 |
| effect of the enamel matrix derivative emdogain on the growth of periodontal pathogens in vitro. | the aim of this study was to evaluate the effect of emdogain (emd), used for periodontal regeneration, on the growth of periodontal pathogens like actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia. for comparison, we studied the effect of emd on several microbes associated with other oral diseases as well as its effect on non-pathogenic oral inhabitants. | 2002 | 11846851 |
| effects of instrumentation, irrigation and dressing with calcium hydroxide on infection in pulpless teeth with periapical bone lesions. | the aim of this study was to evaluate the fate of microorganisms in root canals of teeth with infected pulps and periapical bone lesions with and without the use of calcium hydroxide medication. | 2002 | 11858203 |
| dipeptide utilization by the periodontal pathogens porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens and fusobacterium nucleatum. | porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens and fusobacterium nucleatum, which can frequently be isolated from periodontal pockets, preferentially utilize proteins and peptides as growth substrates. in this study, we determined the size of peptide that is preferentially utilized as a source of energy and material for cell growth by p. gingivalis, p. intermedia, p. nigrescens and f. nucleatum using various sizes of poly amino acids consisting of two to approximately 100 ... | 2002 | 11860556 |
| rapidly expanding lung abscess caused by legionella pneumophila in immunocompromised patients: a report of two cases. | we describe two cases of lung abscess caused by legionella pneumophila in immunocompromised patients. the first case had been treated initially with 60 mg prednisolone for ulcerative colitis, and l. pneumophila serogroup 1 was isolated from sputum samples after cavitation of the lung lesion. the second case was diagnosed as plasma cell lymphoma at post-mortem examination. l. pneumophila serogroup 5 was isolated from the contents of lung abscess, together with enterococcus faecium and prevotella ... | 2002 | 11868601 |
| invasion of vascular cells in vitro by porphyromonas endodontalis. | the objective of this study was to determine whether laboratory strains and clinical isolates of microorganisms associated with root canal infections can invade primary cultures of cardiovascular cells. | 2002 | 12059938 |
| salivary microbiota levels in relation to periodontal status, experience of caries and miswak use in sudanese adults. | the purpose of the present investigation was to assess the salivary levels of 25 oral bacteria in relation to periodontal status and experience of caries, and to compare the levels of these bacteria between habitual miswak and toothbrush users in adult sudanese subjects. | 2002 | 12060423 |
| accelerated alveolar bone loss in hla-b27 transgenic rats: an adult onset condition. | patients with arthritis and crohn's disease may be more susceptible to periodontitis associated alveolar bone loss (abl). hla-b27 transgenic (tg) rats spontaneously develop arthritis and colitis. based on the hypothesis that tg rats would also be susceptible to abl, we compared the naturally occurring abl in tg and fischer 344 wild-type (wt) rats. | 2002 | 12064843 |
| periodontal disease and cardiovascular disease: epidemiology and possible mechanisms. | many early epidemiologic studies reported an association between periodontal disease and cardiovascular disease. however, other studies found no association or nonsignificant trends. this report summarizes the evidence from epidemiologic studies and studies that focused on potential contributing mechanisms to provide a more complete picture of the association between periodontal and heart disease. | 2002 | 12085720 |
| the relationship between the presence of periodontopathogenic bacteria in saliva and halitosis. | to evaluate the association between the presence of periodontal pathogenic bacteria in saliva and halitosis in mouth air. | 2002 | 12090455 |
| pyogenic vertebral osteomyelitis caused by prevotella intermedia. | we describe a case of vertebral osteomyelitis caused by prevotella intermedia, which is an extremely unusual cause of vertebral osteomyelitis. the organism was isolated from vertebral biopsies and the patient was treated successfully with intravenous ampicillin-sulbactam and clindamycin. diagnosis and management of this condition are described, and the importance of anaerobic bacteria in the pathogenesis of vertebral osteomyelitis is discussed. | 2002 | 12111574 |
| upregulation of co-stimulatory molecule expression and dendritic cell marker (cd83) on b cells in periodontal disease. | t cells and their cytokines are well known for their important role in the pathogenesis of periodontitis. to date, the role of antigen presenting cells (apcs), which are known to be critical in the regulation of t cell response, has been poorly investigated in periodontitis. in this study, we analyzed the expression of co-stimulatory molecules (cd80 and cd86) and cd83, which is a marker of mature dendritic cells, on gingival cells that were isolated from severe periodontitis tissues, with the us ... | 2002 | 12113551 |
| actinobacillus actinomycetemcomitans proportion of subgingival bacterial flora in relation to its clonal type. | we investigated whether certain actinobacillus actinomycetemcomitans clones occur in elevated proportions in subgingival flora, and if the proportions relate to other bacteria in the samples. a total of 121 a. actinomycetemcomitans strains from 121 patients with periodontitis were serotyped and 60 strains were also genotyped. the 121 strains were divided into three groups and the 60 strains into two groups according proportion of a. actinomycetemcomitans. the samples from the 60 patients with ge ... | 2002 | 12120706 |
| phylogenetic analysis of prevotella nigrescens, prevotella intermedia and porphyromonas gingivalis clinical strains reveals a clear species clustering. | prevotella nigrescens, prevotella intermedia and porphyromonas gingivalis are oral pathogens from the family bacteroidaceae, regularly isolated from cases of gingivitis and periodontitis. in this study, the phylogenetic variability of these three bacterial species was investigated by means of 16s rrna (rrs) gene sequence comparisons of a set of epidemiologically and geographically diverse isolates. for each of the three species, the rrs gene sequences of 11 clinical isolates as well as the corre ... | 2002 | 12148655 |
| [detection of periodontal pathogenic microorganisms in atheromatous plaque. preliminary results]. | recent studies suggest that chronic infections, including those associated with periodontitis, increase the risk for coronary vascular disease. we hypothesize that oral microorganisms including periodontal bacterial pathogens enter the blood stream during transient bacteremias where they may play a role in the development and progression of atherosclerosis. | 2002 | 12149943 |
| contamination of interdental brushes by periodontopathogens. | experimental studies have shown that intraoral transmission of bacteria can occur. of course, the question arises as to how this transmission may happen. in this study, the contamination of interdental brushes by periodontopathogens is examined and compared to the microbial load of the periodontal pockets. in ten untreated chronic periodontitis patients, four interdental sites were professionally brushed with one interdental brush per patient. subsequently, samples from the depths of the pockets ... | 2002 | 12166717 |
| matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and proteomics: a new era in anaerobic microbiology. | genome sequence data provide a framework for predicting potential microbial activities; however, the proteome content of the cell dictates its response to its environment. microbiology is witnessing a major initiative to elucidate the nature of the proteome of large numbers of species. the tool driving the proteomic revolution is matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. during the analysis process, proteins are ionized and separated on the basis of their mass ... | 2002 | 12173110 |
| microbiological findings and host response in patients with peri-implantitis. | the aim of the present study was to characterise microbiota and inflammatory host response around implants and teeth in patients with peri-implantitis. we included 17 partly edentulous patients with a total of 98 implants, of which 45 showed marginal bone loss of more than three fixture threads after the first year of loading. nineteen subjects with stable marginal tissue conditions served as controls. oral hygiene, gingival inflammation, and probing pocket depth were evaluated clinically at tee ... | 2002 | 12175371 |
| two-stage imz implants and iti implants inserted in a single-stage procedure. a prospective comparative study. | the aim of this study was to evaluate the feasibility of using a two-stage implant system in a single-stage procedure and to study the impact of the microgap at crestal level and to monitor the microflora in the peri-implant area. forty edentulous patients (cawood & howell class v-vi) participated in this study. after randomisation, 20 patients received two imz implants inserted in a single-stage procedure and 20 patients received two iti implants. after 3 months, overdentures were fabricated, s ... | 2002 | 12175374 |
| susceptibility of prevotella intermedia/prevotella nigrescens (and porphyromonas gingivalis) to propolis (bee glue) and other antimicrobial agents. | black-pigmented gram-negative anaerobes such as porphyromonas gingivalis and prevotella intermedia are suspected pathogens in adult periodontitis, whereas prevotella nigrescens has been associated with health. antimicrobial resistance among bacteria from this group has been reported in the past decade. this research aimed to evaluate and compare the susceptibility profile of 17 p. intermedia/p. nigrescens isolates recovered from patients with periodontitis and three reference strains to six anti ... | 2002 | 16887668 |
| relationship between periodontal pocket sulfide levels and subgingival species. | many species implicated in the pathogenesis of periodontal disease produce volatile sulfur compounds (vsc). this investigation examined the relationship between levels of sulfide and subgingival bacterial species in the same periodontal pockets. | 2003 | 14761124 |
| microbiologic diagnostics at titanium implants. | the microbiota found at periimplant lesions have been shown to contain putative periodontal pathogens as well as opportunistic species such as staphylococcus spp, enterics, and candida spp. therefore, a microbiologic diagnosis may be of value as guidance before treatment of such lesions. | 2003 | 15127993 |
| dna probe analysis in smoker and non smoker rapidly progressive periodontitis patients--a pilot study. | smoking is one of the most significant risk factors in the development and further advancement of inflammatory periodontal disease. the bacteria a. actinomycetemcomitans, p. gingivalis and p. intermedius as indicated as the potential pathogens associated with periodontal disease. since the bacteria mentioned as well as smoking are factors associated with periodontitis it is of importance to elucidate the interrelationship between these factors. the purpose of this study was to investigate the pr ... | 2003 | 15328997 |
| interleukin-10 receptor expression in human dental pulp cells in response to lipopolysaccharide from prevotella intermedia. | interleukin-10 receptor (il-10r) expression in human, dental pulp, fibroblast cultures was investigated by using the reverse transcriptase-polymerase chain reaction (rt-pcr) technique. after exposure to lipopolysaccharide (lps) from prevotella intermedia, the il-10r mrna levels increased after 4 h, peaked at 7 h, and dropped back to the unstimulated level at 24 h. maximal production of the il-10r protein in dental pulp fibroblast cultures was detected by western blot analysis after 12 h of lps s ... | 2003 | 12540220 |
| antimicrobial activity of ca(oh)2 dental cements: an in vitro study. | the effect of five commercial calcium hydroxide root canal medications (xr-spad, calcicur, hy-cal, root-cal, hypo-cal) and two calcium hydroxide mixtures (with water or glycerin) on the growth of (a) three anaerobic bacteria associated with endodontic infections; and (b) saliva bacterial strains was determined using the agar diffusion inhibitory test. statistically significant zones of bacterial growth inhibition for all the bacteria tested were observed. calcium hydroxide mixed with glycerin sh ... | 2003 | 12540221 |
| antimicrobial activity of magainin analogues against anaerobic oral pathogens. | magainins are a family of potent antimicrobial cationic peptides that possess antimicrobial activity against a wide range of target organisms. in this study, the antimicrobial activity of synthetic magainin-mimetic compounds msi-751 and msi-774 was investigated against the periodontal pathogens porphyromonas gingivalis, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, eikenella corrodens, prevotella loescheii and prevotella intermedia. p. gingivalis was more susceptible to msi-751 ... | 2003 | 12507842 |
| subgingival microbiota levels and their associations with periodontal status at the sampled sites in an adult sudanese population using miswak or toothbrush regularly. | little information is available on the effect of miswak use on gingival microbiota. we assessed levels of 28 oral bacteria in subgingival plaque of adult sudanese miswak (n = 38) and toothbrush users (n = 36) age range 20-53 years (mean 34.6 years) to study associations between these bacteria, oral hygiene method, and periodontal status at the sampled sites. a pooled subgingival plaque sample from 6 probing sites of 1 selected tooth in each jaw was obtained from each subject. whole genomic dna p ... | 2003 | 12790510 |
| acquisition and loss of porphyromonas gingivalis, actinobacillus actinomycetemcomitans and prevotella intermedia over a 5-year period: effect of a triclosan/copolymer dentifrice. | the present study describes the natural history of porphyromonas gingivalis, actinobacillus actinomycetemcomitans and prevotella intermedia over a 5-year period and the effect of a triclosan/copolymer dentifrice on these organisms in a normal adult population. | 2003 | 12795792 |
| genomics of oral bacteria. | advances in bacterial genetics came with the discovery of the genetic code, followed by the development of recombinant dna technologies. now the field is undergoing a new revolution because of investigators' ability to sequence and assemble complete bacterial genomes. over 200 genome projects have been completed or are in progress, and the oral microbiology research community has benefited through projects for oral bacteria and their non-oral-pathogen relatives. this review describes features of ... | 2003 | 12799321 |
| microbiologic and radiographic analysis of ligature-induced peri-implantitis with different dental implant surfaces. | the goal of this study was to evaluate microbiota and radiographic peri-implant bone loss associated with ligature-induced peri-implantitis. | 2003 | 12814313 |
| lethal photosensitization in microbiological treatment of ligature-induced peri-implantitis: a preliminary study in dogs. | this pilot study evaluated, by culture testing, the effectiveness of lethal photosensitization for the microbiological treatment of peri-implantitis in dogs. experimental peri-implantitis was induced by ligature placement for 2 months. following ligature removal, plaque control was instituted by scrubbing with 0.12% chlorhexidine daily for 12 months. subsequently, mucoperiosteal flaps were elevated for scaling the implant surface. microbial samples were obtained with paper points before and afte ... | 2003 | 12816360 |
| lethal photosensitization of periodontal pathogens by a red-filtered xenon lamp in vitro. | the ability of helium-neon (he-ne) laser irradiation of a photosensitizer to induce localized phototoxic effects that kill periodontal pathogens is well documented and is termed photodynamic therapy (pdt). | 2003 | 12828662 |
| application of denaturing gradient gel electrophoresis (dgge) to the analysis of microbial communities of subgingival plaque. | denaturing gradient gel electrophoresis (dgge) was applied to the microbiologic examination of subgingival plaque. | 2003 | 12828664 |
| presence of the cfxa gene in bacteroides distasonis. | in this study we investigated the presence of the cfxa gene (encoding a class a beta-lactamase) in 73 strains of the bacteroides fragilis group belonging to the species b. distasonis (34), b. vulgatus (14), b. thetaiotaomicron (8), b. merdae (6), b. caccae (9) and b. ovatus (2) isolated from human intestinal microflora of healthy children and adults. employing specific primers to the cfxa gene, a 312-bp amplified fragment was obtained in 2 strains of b. vulgatus and 9 strains, the majority from ... | 2003 | 12837513 |
| the haem pigment of the oral anaerobes prevotella nigrescens and prevotella intermedia is composed of iron(iii) protoporphyrin ix in the monomeric form. | the haem pigment of porphyromonas gingivalis is composed of micro -oxo bishaem, [fe(iii)ppix](2)o, but the nature of that generated by prevotella species has not been established. mössbauer, raman and uv-visible spectrophotometry were used to characterize the haem pigment of prevotella intermedia and prevotella nigrescens. mössbauer and raman spectroscopy revealed the major haem species to be monomeric iron protoporphyrin ix, fe(iii)ppix.oh (haematin). the terminal growth ph of both species on b ... | 2003 | 12855722 |
| positive and negative bacterial associations involving dialister pneumosintes in primary endodontic infections. | dialister pneumosintes is an anaerobic gram-negative rod that has been recently implicated as a candidate endodontic pathogen. in this study, samples taken from abscessed teeth and infected root canals associated with asymptomatic or symptomatic periradicular lesions were examined for the occurrence of bacterial associations involving d. pneumosintes. dna was extracted from the samples, and the presence of d. pneumosintes and 16 other bacterial species was determined by means of species-specific ... | 2003 | 12877258 |
| accelerated alveolar bone loss in mice lacking interleukin-10. | interleukin-10 regulates pro-inflammatory cytokines, including those implicated in alveolar bone resorption. we hypothesized that lack of interleukin-10 leads to increased alveolar bone resorption. male interleukin-10(-/-) mice, on 129/svev and c57bl/6j background, were compared with age-, sex-, and strain-matched interleukin-10(+/+) controls for alveolar bone loss. immunoblotting was used for analysis of serum reactivity against bacteria associated with colitis and periodontitis. interleukin-10 ... | 2003 | 12885849 |
| antibiotic resistance of pathogenic bacteria from odontogenic infections in taiwan. | the purpose of this study was to evaluate the susceptibility of facultative and anaerobic odontogenic infectious flora to various antibiotics. we assessed 178 bacterial strains isolated from 74 patients with odontogenic infections. the e-test was used to determine susceptibility. the microbial flora was predominantly facultative gram-positive organisms and anaerobic gram-negative bacilli. the results of antimicrobial susceptibility test showed that ampicillin resistance was found with a very hig ... | 2003 | 12886961 |
| microbiological analysis of infected root canals from symptomatic and asymptomatic teeth with periapical periodontitis and the antimicrobial susceptibility of some isolated anaerobic bacteria. | the purpose of the present study was to investigate the correlation between the composition of the bacterial flora isolated from infected root canals of teeth with apical periodontitis with the presence of clinical signs and symptoms, and to test the antibiotic susceptibility of five anaerobic bacteria mostly commonly found in the root canals of symptomatic teeth against various substances using the e-test. microbial samples were taken from 48 root canals, 29 symptomatic and 19 asymptomatic, usi ... | 2003 | 12930519 |
| sensitivity of genera porphyromonas and prevotella to the bactericidal action of c-terminal domain of human cap18 and its analogues. | this paper reports the effect of the synthesized 27-amino acid sequence in the c-terminal domain of human cap18 (hcap18), a human cationic antibacterial protein or cathelicidin, on certain strains belonging to the genera porophyromonas and prevotella. the domain binds lipopolysaccharides (lps) from porophyromonas gingivalis and porophyromonas circumdentaria as well as enterobacterial lps. two analogues of hcap18, designated ll/cap18 and ff/cap18, were also tested to determine whether additional ... | 2003 | 12930528 |
| coaggregation of streptococcus salivarius with periodontopathogens: evidence for involvement of fimbriae in the interaction with prevotella intermedia. | streptococcus salivarius is divided into two serological subgroups that carry either fibrils or fimbriae. although fimbriae have been observed on up to 50% of s. salivarius strains in the human oral cavity, no function has yet been assigned to them. to determine whether s. salivarius fimbriae have a role in adhesion, we examined the ability of s. salivarius to coaggregate with selected microorganisms involved in periodontal diseases. our results show that s. salivarius coaggregated with fusobact ... | 2003 | 12930529 |
| coronal microleakage assessed by polymicrobial markers. | studies have shown significant bacterial leakage following exposure of sealed root canals to artificial and natural saliva. the objective of this study was to determine contamination via bacterial migration in artificial saliva in endodontically treated teeth using different obturation techniques and sealers. a total of 144 extracted, human mandibular anterior teeth were cleaned and shaped to a #40 master apical file using hand and rotary instrumentation. one hundred and twenty teeth were divide ... | 2003 | 12937592 |
| prevotella intermedia meningitis associated with cerebrospinal fluid leakage in an adolescent. | 2003 | 12938679 | |
| the subgingival microflora in phenytoin-induced gingival hyperplasia. | microbial flora and gingival conditions were compared between a group of patients with phenytoin-induced gingival hyperplasia as a test group, a control group of patients who were administered phenytoin without gingival hyperplasia and a blank group who took no phenytoin and no gingival hyperplasia in mentally retarded patients. | 2003 | 12941071 |
| potential role of vascular endothelial growth factor, interleukin-8 and monocyte chemoattractant protein-1 in periodontal diseases. | host-mediated immunoinflammatory pathways activated by bacteria lead to destruction of the periodontal connective tissues and alveolar bone. the objective of this study was to elucidate the activation of the inflammatory processes in periodontal disease by quantitative assessment of cytokines and periodontopathogens. gingival crevicular fluids (gcf) and subgingival plaque samples were collected from patients with chronic periodontitis and gingivitis and from periodontally healthy sites. vascular ... | 2003 | 12962428 |
| immune response to fusobacterium nucleatum and prevotella intermedia in the sputum of patients with acute exacerbation of chronic bronchitis. | to assess the role of anaerobic bacteria in acute exacerbation of chronic bronchitis (aecb). | 2003 | 12970005 |
| quantitative real-time pcr using taqman and sybr green for actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tetq gene and total bacteria. | accurate quantification of bacterial species in dental plaque is needed for microbiological diagnosis of periodontal diseases. the present study was designed to assess the sensitivity, specificity and quantitativity of the real-time pcr using the geneamp sequence detection system with two fluorescence chemistries. taqman probe with reporter and quencher dye, and sybr green dye were used for sources of the fluorescence. primers and probes were designed for actinobacillus actinomycetemcomitans, po ... | 2003 | 14557000 |
| one case of gram-negative anaerobic spondylodiscitis with prevotella intermedia. | we report the case of a 45-year-old woman with spondylodiscitis at l1/l2, communicating with paravertebral, intravertebral and bilateral psoas abscesses. percutaneous computed tomography (ct)-guided abscess drainage and an intravenous antibiotic therapy with imipenem were performed. after removing the drainage at 2 weeks, the patient was discharged at 4 weeks with normalized blood parameters, normal temperature, and without need for analgesics. the underlying bacterium in the case was a very rar ... | 2003 | 14574605 |
| distribution of periodontal pathogens in korean aggressive periodontitis. | microbial associations in aggressive periodontitis versus different ethnic origins are substantially unknown. we undertook this study to determine the prevalence of seven putative periodontopathogens in korean patients and to evaluate microbial differences in localized and generalized aggressive periodontitis patients. | 2003 | 14584866 |
| flavobacterium johnsoniae gldh is a lipoprotein that is required for gliding motility and chitin utilization. | cells of flavobacterium johnsoniae move rapidly over surfaces by gliding motility. the mechanism of this form of motility is not known. six genes (glda, gldb, gldd, gldf, gldg, and ftsx) that are required for gliding have been described. tn4351 mutagenesis was used to identify another gene, gldh, which is required for cell movement. gldh mutants formed nonspreading colonies, and individual cells lacked the cell movements and ability to propel latex spheres along their surfaces that are character ... | 2003 | 14594839 |
| immunoglobulin g and a antibody responses to bacteroides forsythus and prevotella intermedia in sera and synovial fluids of arthritis patients. | the objective of the present study was to investigate immunoglobulin g (igg) and iga antibody immune responses to porphyromonas gingivalis, prevotella intermedia, bacteroides forsythus, and candida albicans in the sera of patients with rheumatoid arthritis (ra), the synovial fluid (sf) of patients with ra (ra-sf samples), and the sf of patients without ra (non-ra-sf samples). an enzyme-linked immunosorbent assay was used to determine igg and iga antibody levels in 116 serum samples from patients ... | 2003 | 14607865 |
| induction of interleukin-8 gene expression by black-pigmented bacteroides in human pulp fibroblasts and osteoblasts. | to investigate the effect of black-pigmented bacteroides on the expression of interleukin (il)-8 gene in human pulp fibroblasts and osteoblasts. | 2003 | 14641442 |
| antagonistic interactions among fusobacterium nucleatum and prevotella intermedia with oral lactobacilli. | microbiota residing in oral ecosystems show great complexity because of the mutual interdependence of certain microorganisms that might influence their presence or absence at a given site. the aim of this work was to evaluate possible antagonistic interactions among fusobacterium nucleatum and prevotella intermedia, isolated from periodontal pockets, and lactobacillus casei, l. salivarius, l. rhamnosus and l. plantarum isolated from saliva. different tests were carried out using each strain as a ... | 2003 | 14643404 |
| hemolytic activity of prevotella intermedia and prevotella nigrescens strains: influence of abiotic factors in solid and liquid assays. | the influence of growth medium, hemin and menadione, blood source and atmosphere of incubation on the expression of hemolytic activity of 25 strains of prevotella intermedia and prevotella nigrescens was evaluated. the best hemolytic activity was observed for samples of both species growing in brain heart infusion agar and incubated in brewer-like anaerobic jars for 48 h. hemolysis was less intense and occurred later in the presence of hemin and menadione in solid media. beta-hemolysis was detec ... | 2003 | 12576156 |
| periodontal status in an italian young adult population. prevalence and relationship with periodontopathic bacteria. | to determine the prevalence of periodontitis in an italian young adult population and the relationship with actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia in subgingival plaque. a full-mouth periodontal and oral examination was performed in 70 subjects. dental and behaviour habits were assessed with a standardised questionnaire. subgingival plaque samples were collected from the deepest pocket of the first molars in each quadrant with a sterile curette. ... | 2003 | 12578311 |
| isolation and characterization of hemolysin activated by reductant from prevotella intermedia. | the hemolysin from prevotella intermedia was partially purified from culture supernatant and then characterized. the hemolysin produced a clear beta-hemolytic zone on a blood agar plate. hemolytic activity was 2.5-fold greater in culture supernatant compared to that cell-associated. the isolation and purification procedure involved ammonium sulfate and polyethylene glycol precipitations and ion-exchange chromatographies on deae-sephacel and cm-sepharose. the activity of this hemolysin was stimul ... | 2003 | 12589956 |
| high prevalence of helicobacter pylori detected by pcr in the oral cavities of periodontitis patients. | helicobacter pylori has been associated with the development of peptic ulcers and gastric cancer. although it may be transmitted through the oral cavity, it is unknown whether the oral cavity acts as a permanent reservoir for this bacterium. the purpose of this study was to use nested polymerase chain reaction (pcr) to clarify whether the oral cavity acts as a reservoir for h. pylori. | 2003 | 12593608 |
| antibacterial effects of resinous retrograde root filling materials. | the effect of three resinous retrograde root filling materials on the growth of four obligate anaerobic bacteria associated with endodontic infections was determined by using the agar diffusion inhibitory test. samples of the following resinous materials (resin-modified, glass-ionomer cement, fuji ii lc; compomer, dyract; and composite resin, spectrum) were incubated for 1 week with the following anaerobic bacteria: fusobacterium nucleatum, porphyromonas gingivalis, p. endodontalis, and prevotel ... | 2003 | 12597711 |
| faropenem, a new oral penem: antibacterial activity against selected anaerobic and fastidious periodontal isolates. | the in vitro activity of faropenem, an oral penem, was compared with those of penicillin, co-amoxiclav, cefoxitin, clindamycin, erythromycin and metronidazole against 106 isolates of anaerobic pathogens involved in systemic infections. the organisms tested comprised porphyromonas gingivalis (29), prevotella spp. (eight), prevotella melaninogenica (seven), prevotella intermedia (five), actinomyces spp. (25), fusobacterium nucleatum (14), peptostreptococcus spp. (11), bacteroides ureolyticus (five ... | 2003 | 12615878 |
| kinetic and site-directed mutagenesis studies of prevotella intermedia acid phosphatase. | site-directed mutagenesis was used to examine the roles of the conserved histidine, arginine and cysteine residues in acid phosphatase from prevotella intermedia (piacp). the replacement of histidine and arginine residues resulted in the elimination of the piacp activity while the cysteine mutants retained activity. these results suggest that the histidine and arginine residues are essential for catalysis. | 2003 | 12625826 |
| bactericidal effects of different laser wavelengths on periodontopathic germs in photodynamic therapy. | this study was an attempt to clarify whether the bactericidal effects of photodynamic therapy (pdt) are wavelength or dose-dependent. we also attempted to create an optimised protocol for a light-based bactericidal modality to eliminate periodontal pathogens. cultures of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphromonas gingivalis, prevotella intermedia, and streptococcus sanguis, were exposed to a he-ne laser (632.8 nm) with a 30 mw power output, a 100 mw diode laser a ... | 2003 | 12627274 |
| beta-lactamase production in prevotella and in vitro susceptibilities to selected beta-lactam antibiotics [corrected]. | this study looked for beta-lactamase production in 100 prevotella isolates. mics were determined for amoxycillin, ticarcillin, amoxycillin+clavulanate, cephalothin, cefuroxime, cefixime, cefpodoxime and cefotaxime using the reference agar dilution method (standard m11 a4, nccls). beta-lactamase activity was detected in 58 of the 100 isolates, 24 of 46 black-pigmented provotella and 34 of 54 non-pigmented prevotella. all beta-lactamase-negative strains were susceptible to all beta-lactam antibiot ... | 2003 | 12636990 |
| helicobacter pylori and campylobacter rectus share a common antigen. | aim: the aim of this study was to investigate the presence of antigens with immunological cross-reactivity in periodontopathogenic bacteria and helicobacter pylori, the pathogen associated with gastritis and peptic ulcers in human. materials and methods/results: among the putative periodontopathogens tested (actinobacillus actinomycetemcomitans, campylobacter rectus, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia and treponema denticola), cross-reactive bands were only ... | 2003 | 12654095 |
| acid-neutralizing activity during amino acid fermentation by porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum. | acid-neutralizing activity during amino acid fermentation by washed cells of porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum was studied. when the washed cells of these strains were anaerobically incubated in the presence of aspartylaspartic acid or glutamylglutamic acid for p. gingivalis, aspartic acid for p. intermedia and glutamic acid for f. nucleatum at an initial ph of 5.0 or 5.5, the ph of the incubation mixtures rose toward neutral. f. nucleatum had the highes ... | 2003 | 12654101 |
| microorganisms from canals of root-filled teeth with periapical lesions. | the objective of the present study was to identify the microbial flora within root canals of teeth with failed root-canal treatment and to determine the association of the various species with clinical features. | 2003 | 12656508 |
| proteolytic and hydrolytic enzymes from putative periodontal pathogens: characterization, molecular genetics, effects on host defenses and tissues and detection in gingival crevice fluid. | 2003 | 12656998 | |
| occurrence of periodontal bacteria in healthy children: a 2-year longitudinal study. | to examine the occurrence of specific periodontal bacteria in children and adolescents. | 2003 | 14986909 |
| induction of cyclooxygenase-2 mrna and protein expression in human pulp cells stimulated with black-pigmented bacteroides. | cyclooxygenase-2 (cox-2) is induced after the activation of cells by a variety of proinflammatory agents. recently, evidence has shown that cox-2 may play a role in the pathogenesis of pulpal inflammation. however, little is known regarding the mechanism of pulpal inflammation at the site of bacterial infection. the purpose of this study was to determine the effects of the supernatants from black-pigmented bacteroides (porphyromonas endodontalis, porphyromonas gingivalis, and prevotella intermed ... | 2003 | 12701770 |
| ovine periodontitis as a potential model for periodontal studies. cross-sectional analysis of clinical, microbiological, and serum immunological parameters. | : to investigate infection and host immunity patterns in sheep with naturally occurring "broken-mouth" periodontitis. | 2003 | 12702113 |
| the effect of a triclosan-containing dentifrice on the progression of periodontal disease in an adult population. | the aim of the present study was to determine the effect of unsupervised, long-term use of a 0.3% triclosan/2% copolymer dentifrice on the progression of periodontal disease in a general adult population. | 2003 | 12716333 |
| herpesviral-bacterial interactions in aggressive periodontitis. | human cytomegalovirus, epstein-barr virus and herpesvirus co-infections occur with significantly higher frequency in actively progressing than in stable periodontitis sites of adolescents and young adults. also, periodontal presence of cytomegalovirus and epstein-barr virus is associated with increased occurrence of subgingival porphyromonas gingivalis, bacteroides forsythus, dialister pneumosintes, prevotella intermedia, prevotella nigrescens, treponema denticola and actinobacillus actinomycete ... | 2003 | 12716334 |
| the effects of a new mouthrinse containing chlorhexidine, cetylpyridinium chloride and zinc lactate on the microflora of oral halitosis patients: a dual-centre, double-blind placebo-controlled study. | this study evaluated the microbial effects of a newly formulated mouthwash (halita) on oral halitosis patients. | 2003 | 12716335 |