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progressive gorham disease of the forearm.gorham's-stout disease is a rare but potentially debilitating disease consisting of massive bone osteolysis and bone resorption associated with vascular proliferation and increased osteoclastic activity. although it can present in a wide variety of forms, it typically involves bones formed by intramembranous ossification such as the skull, pelvis, and scapula. it can occur spontaneously or after trauma. most cases are monofocal and resolved spontaneously, although there are reports of multifocal ...200819292225
red 'anjou' pear has a higher photoprotective capacity than green 'anjou'.photoprotective function of anthocyanins along with xanthophyll cycle and antioxidant system in fruit peel was investigated in red 'anjou' vs green 'anjou' pear (pyrus communis) during fruit development and in response to short-term exposure to high light. the sun-exposed peel of red 'anjou' had higher maximum quantum yield of photosystem ii (f(v)/f(m)) than that of green 'anjou' and both the sun-exposed peel and the shaded peel of red 'anjou' had smaller decreases in f(v)/f(m) after 2-h high li ...200818715235
a lethal form of gorham disease associated with extensive musculoskeletal pneumatosis: case report and review of the literature.we report here the imaging findings of a rare case of a lethal form of gorham disease in a young female patient. multimodality imaging findings over 13 year-follow-up demonstrated progressive wide spread skeletal and soft tissue abnormalities with permeative osteolysis, pathological fractures and severe skeletal deformities. unusual extensive osseous and soft tissue pneumatosis was illustrated on cross-sectional studies. the progressive nature of this form of gorham disease and the subsequent co ...200818629462
factors involved in alleviating water stress by partial crop removal in pear trees.we studied the relief of water stress associated with fruit thinning in pear (pyrus communis l.) trees during drought to determine what mechanisms, other than stomatal adjustment, were involved. combinations of control irrigation (equal to crop water use less effective rainfall) and deficit irrigation (equal to 20% of control irrigation), fruit load (unthinned and thinned to 40 fruits per tree) and root pruning (pruned and unpruned) treatments were applied to pear (cv. 'conference') trees during ...200818595850
enhancement of spermidine content and antioxidant capacity in transgenic pear shoots overexpressing apple spermidine synthase in response to salinity and hyperosmosis.in our previous work, an apple spermidine synthase (spds)-overexpressing transgenic european pear (pyrus communis l. 'ballad'), line no. 32 (#32), demonstrated attenuated susceptibility to stress treatment. in the current paper, changes in enzymatic and non-enzymatic antioxidant capacity of the transgenic pear (line #32) were investigated in response to nacl or mannitol stress. under non-stressed conditions (before stress treatment), spermidine (spd) contents and spds activity of line #32 were h ...200818586287
idiopathic calvarial thinning.an 82-year-old diabetic female had been aware of gradually enlarging, painless scalp depressions in the bilateral parietal regions for more than 6 years. she had no history of head injury, or inflammatory or malignant disease. her family history was unremarkable for hereditary bone disease. she had diabetes mellitus which had been well controlled with orally administered drugs. blood examination showed normal renal and liver functions with normal serum calcium, magnesium, and phosphorus concentr ...200818574336
gorham disease of the mandible: radiographic findings and radiotherapy response.gorham disease, a rare condition of unknown etiology, is characterized histologically by bone disintegration and endothelial proliferation. we describe serial imaging findings, the treatment course, and radiotherapy response in a patient with pathologically confirmed gorham disease involving the right mandible. progressive mandibular resorption was managed initially with multiple surgical resections and reconstruction, followed by external-beam radiotherapy, which was an effective treatment meth ...200818561108
gorham's disease: an osseous disease of lymphangiogenesis?gorham's disease, also known as massive osteolysis, gorham-stout disease, vanishing bone disease, or, phantom bone disease is a rare disorder of the musculoskeletal system. the disease is characterized by osteolysis in bony segments, with localized proliferation of lymphatic channels. the presence of abundant, leaky systemic lymphatic vessels is often accompanied by chylous ascites. there is no standardized treatment available for gorham's disease, and its molecular mechanisms remain unclear. fu ...200818519972
a new gene for resistance to dysaphis pyri in pear and identification of flanking microsatellite markers.dysaphis pyri is an important aphid pest of european pear (pyrus communis) cultivars, none of which are currently reported to be resistant. in this study, we produced a progeny of the european pear comice crossed with an accession of snow pear (pyrus nivalis) that segregated for resistance to d. pyri in a mendelian fashion, indicating the presence of a major gene, dp-1. following screening of the parents and seedlings with microsatellite markers, cosegregation analysis indicated that dp-1 is fla ...200819088815
diffuse lymphangiomatosis--a fatal case with atypical skeletal features.diffuse lymphangiomatosis is a rare idiopathic condition that occurs mostly in children, is characterized by a non-neoplastic proliferation of lymphatic vessels, leading to organ dysfunction, chylous effusions, and death. a closely related condition-the gorham-stout syndrome-is also characterized by lymphangiomatosis and chylous effusions, but also with massive osteolytic changes ("vanishing bone disease"). a 33-year-old woman presented with a 5-year history of worsening chylous effusions and or ...200819011406
[gorham-stout disease].gorham-stout disease is a rare entity characterised by a massive osteolysis and typically starts monocentrically in an isolated bone. concerning the pathogenesis, haemangiomatosis and an overstimulation of osteoclasts are discussed which cause a progressive destruction of the affected skeleton and involve neighbouring soft tissue in the further progress of the disease.200818846495
disappearing bones: a case of gorham-stout disease. 200818446639
an investigation of the psychometric properties of the mmpi-2 restructured clinical (rc) scales with mental health inpatients.in this study, we compared the internal psychometric properties and external correlates of the mmpi-2 (butcher et al., 2001; tellegen et al., 2003) restructured clinical (rc) scales and corresponding mmpi-2 non-k-corrected clinical scales in a sample of 280 men and 264 women admitted to inpatient psychiatric units. we evaluated the internal psychometric properties of the rc and clinical scales by intercorrelation analyses and alpha coefficients. we investigated external correlates of scores from ...200818444120
[a rare differential diagnosis of chylothorax].we present the case report of a 17-year-old patient with right pleural effusion and pre-existing lymphangiomas of the chest wall. thoracentesis revealed a salmon-milky effusion containing chylomicrones defining a chylothorax (with blood intermixture). a ct scan and mri showed multiple osteolysis. after excluding malignancy as well as infectious and traumatic causes of the chylothorax, we made the diagnosis of a gorham-stout syndrome. a minimal-invasive pleurectomy and decortication were done suc ...200818264894
three-dimensional gas exchange pathways in pome fruit characterized by synchrotron x-ray computed tomography.our understanding of the gas exchange mechanisms in plant organs critically depends on insights in the three-dimensional (3-d) structural arrangement of cells and voids. using synchrotron radiation x-ray tomography, we obtained for the first time high-contrast 3-d absorption images of in vivo fruit tissues of high moisture content at 1.4-microm resolution and 3-d phase contrast images of cell assemblies at a resolution as low as 0.7 microm, enabling visualization of individual cell morphology, c ...200818417636
efficacy results of insecticides against cydia pomonella, the codling moth, in belgium during the last decade (1998-2007).the codling moth is an economically important top fruit pest, with its major flight from may till august. we give an overview of ten years of results of efficacy field trials against the codling moth cydia pomonella, with a number of commonly used products (diflubenzuron, fenoxycarb, flufenoxuron, indoxacarb, granulosis virus, methoxyfenozide and tebufenozide). all trials were executed according to eppo guidelines. trials were performed in the belgian fruit growing area nearby sint-truiden, on a ...200819226808
field evaluations of concentrated spray applications of microencapsulated sex pheromone for codling moth (lepidoptera: tortricidae).the application of a microencapsulated (mec) sex pheromone formulation (checkmate cm-f) for codling moth, cydia pomonella (l.), in low volume, concentrated sprays was evaluated in a series of small plot and grower trials in apple, malus domestica borkhausen, and pear, pyrus communis l. preliminary tests found that mec sprays applied at 172-207 kilopascals in 12-23 liters/ha deposited the highest density of microcapsules per leaf. the addition of a latex sicker did not increase the deposition of ...200818801264
diurnal variation of walnut tree volatiles and electrophysiological responses in cydia pomonella (lepidoptera: tortricidae).in recent years, many studies have been carried out on the behavioural and electrophysiological responses of cydia pomonella (l.) to host volatile emissions, to find alternative attractants to the sex pheromone for pest monitoring. these studies have focused on apple and pear, and very little has been done on walnut. in the present work, the diurnal and seasonal variation in walnut volatile emissions and the electrophysiological response of c. pomonella have been studied.200818300208
multiresidue method for the determination of residues of 251 pesticides in fruits and vegetables by gas chromatography/mass spectrometry and liquid chromatography with fluorescence detection.a method is described for the determination of 251 pesticide and degradation product residues in fruit and vegetable samples. extraction of the sample with acetonitrile is followed by a salting-out step. co-extractives are removed by passing a portion of the acetonitrile extract through an octadecyl (c18) solid-phase extraction cleanup cartridge and then, in a second cleanup, through a carbon cartridge coupled to an amino propyl cartridge. determination is by gas chromatography with mass-selecti ...200810868594
ampullary organs and electroreception in freshwater carcharhinus leucas.the ampulla of lorenzini of juvenile carcharhlinus leucas differ histologically from those previously described for other elasmobranchs. the wall of the ampullary canal consists of protruding hillock-shaped epidermal cells that appear to secrete large quantities of a mucopolysaccharide gel. the ampullary organs comprise a long canal sheathed in collagen terminating in an ampulla. each ampulla contains six alveolar sacs, with each sac containing hundreds of receptor cells. the receptor cells are ...200814692487
in vitro digestion of betalainic foods. stability and bioaccessibility of betaxanthins and betacyanins and antioxidative potential of food digesta.betalains are considered to be bioactive dietary phytochemicals. the stability of betacyanins and betaxanthins from either fresh foods or manufactured products of cactus pear fruit ( opuntia ficus indica l. mill. cv. gialla and rossa) and red beet ( beta vulgaris l. ssp. vulgaris) was assessed in a simulated oral, gastric, and small intestinal digestion and compared with the digestive stability of purified pigments. a minor loss of indicaxanthin, at the gastric-like environment only, and a decre ...200818959410
identification and quantification of betalains from the fruits of 10 mexican prickly pear cultivars by high-performance liquid chromatography and electrospray ionization mass spectrometry.qualitative and quantitative analyses of betalain pigments in 10 cultivars/lines of prickly pear (opuntia spp.) fruit grown in mexico were conducted with reverse phase high-performance liquid chromatography-diode array detection (hplc-dad) coupled with electrospray mass spectrometry (esi-ms). betacyanins and betaxanthins were identified by comparison with the uv/vis and mass spectrometric characteristics as well as the retention times of semisynthesized reference betaxanthins. data revealed that ...200818578538
hypersensitive response and acyl-homoserine lactone production of the fire blight antagonists erwinia tasmaniensis and erwinia billingiae.fire blight caused by the gram-negative bacterium erwinia amylovora can be controlled by antagonistic microorganisms. we characterized epiphytic bacteria isolated from healthy apple and pear trees in australia, named erwinia tasmaniensis, and the epiphytic bacterium erwinia billingiae from england for physiological properties, interaction with plants and interference with growth of e. amylovora. they reduced symptom formation by the fire blight pathogen on immature pears and the colonization of ...200821261861
[prickly pear as an acid absorber]. 20082631173
[detection of fruit juice adulteration in apple and pear juice].a method has been developed to detect the adulteration of pear juice with apples and apple juice with pears. in different varieties of apples and pears and in several commercial juices the flavonoids were examined by hplc with respect to their quality and quantity. the chalcones phloretin glucoside and phloretin xyloglucoside are typical compounds found in apples (detection limit 7 ng). they are suitable indicators for detecting adulteration of pear juice with apples. isohamnetin glucoside canno ...20082718633
occurrence of patulin and its dietary intake through pear, peach, and apricot juices in italy.pear, peach, apricot, and mixed juices (125 samples) were purchased in italian supermarkets and organic food shops and analysed for patulin content. an overall incidence of 34.4% was observed in the fruit juices, with a mean contamination level of 3.6 µg kg(-1). no one sample exceeded 50 µg kg(-1), the maximum permitted limit according to european legislation. however, 19 positive samples contained more than 10 µg kg(-1) of patulin, which is the maximum level permitted for baby food. the inciden ...200824784810
susceptibility of ceratitis capitata wiedemann (diptera: tephritidae) to entomopathogenic fungi.the mediterranean fruit fly ceratitis capitata (wiedemann) is one of the most serious pest of fruits in syria and present all year round. this fly is active on different host plants, such as mango, peach, fig, apple, pear, and citrus. the number of generations per year varies with local temperatures and host plant. the objective of this research was: to evaluate the pathogenicity of entomopathogenic fungi on adults of c. capitata under laboratory conditions. flies for experiments were obtained f ...200819226799
microbial control of insect pests in temperate orchard systems: potential for incorporation into ipm.because of their selectivity and safety, microbial control agents (mcas) appear to be ready-made components of integrated pest management (ipm) systems that do not pose a threat to applicators or the environment and allow other natural enemies to function. control of several orchard pest insects using mcas, including viruses, bacillus thuringiensis, fungi, and entomopathogenic nematodes (epns), have been demonstrated in apple, pear, stone fruits, citrus, and several nut crops. b. thuringiensis i ...200817803454
over-expression of the apple spermidine synthase gene in pear confers multiple abiotic stress tolerance by altering polyamine titers.an apple spermidine synthase (spds) gene (mdspds1) was verified to encode a functional protein by the complementation of the spe3 yeast mutant, which lacks the spds gene. to justify our hypothesis that apple spds is involved in abiotic stress responses and to obtain transgenic fruit trees tolerant to abiotic stresses as well, mdspds1-over-expressing transgenic european pear (pyrus communis l. 'ballad') plants were created by agrobacterium-mediated transformation. a total of 21 transgenic lines s ...200817549601
the study and analysis of the mating behavior and sound production of male cicada psalmocharias alhageos (kol.) (homoptera:cicadidae) to make disruption in mating.psalmocharias alhageos is an important pest of vine in most parts of iran, afghanistan, pakistan, southern areas of russia, turkey and iraq. this cicada is spread in most provinces in iran such as esfahan, hamedan, qazvin, markazi, lorestan, qom, kerman, tehran and kordestan. in addition to vine, this insect damages some other fruit trees, such as apple, sour cherry, quince, peach, pomegranate and pear trees and some non-fruit trees, namely white poplar, ash, elm, eglantine, silk and black popla ...200819266919
[online detection of soluble solids content of pear by near infrared transmission spectrum].the research was to detect soluble solids content (ssc) of pear online by near infrared transmission spectrum. the movement speed of pear was 0.5 m x s(-1) the power of light source was 300 w, and semi-transmission was used to collect the spectrum of pears. the total experiment samples were 187 pears, with a calibration set of 147 pears and a validation set of 40 pears. partial least squares (pls) and principal component regression (pcr) technique were used to develop the calibration model for o ...200819271484
scale insects and mealy bugs (homoptera: coccoidea) attacking deciduous fruit trees in the western north coast of alexandria, egypt.this investigation covered a survey of scale insects and mealy bugs infesting ten growing species of deciduous fruit trees in three localities in alexandria govemorate. these localities were merghem, burg el-arab, and el-nahda about 50 km. west of alexandria under both rain-fed and irrigation system conditions. the common inspected fruit trees were fig, white mulberry, pomegranate, apple, pear, apricot, european plum, peach, almond, and persimmon. it was shown that a group of twenty scale insect ...200819226793
inactivation of listeria monocytogenes in raw fruits by enterocin as-48.the purpose of this study was to determine the effect of enterocin as-48 on listeria monocytogenes cect 4032 in fruits and fruit juice. fruits were contaminated with a l. monocytogenes cell suspension, washed with enterocin as-48 (25 microg/ml) or with sterile distilled water as control, and stored at different temperatures (-20, 6, 15, 22 degrees c). washing treatments significantly inhibited or completely inactivated l. monocytogenes in strawberries, raspberries, and blackberries stored at 15 ...200819244899
[adaptation of mycoplasma gallisepticum to unfavorable growth conditions: changes in morphological and physiological characteristics].adaptation of mycoplasma gallisepticum to unfavorable growth conditions results in altered morphological and physiological characteristics of the cells. m. gallisepticum populations in a complete nutrient medium contain pear-shaped vegetative cells (d approximately 0.3 microm; l approximately 0.8 microm) with pronounced polar and cytoskeleton-like structures. such mycoplasma cells are able to induce damage in a bacterial genome, causing an sos response of the test strain (escherichia coli pq37). ...200819137716
quantitative resistance traits and suitability of woody plant species for a polyphagous scarab, popillia japonica newman.the japanese beetle, popillia japonica newman, has an unusually broad host range among deciduous woody plants, yet it feeds only sparingly, or not at all, on certain species in the field. we evaluated beetles' preference, survival over time and fecundity on eight woody plant species historically rated as susceptible or resistant and, after verifying those ratings, tested whether resistance is correlated with so-called quantitative defense traits including leaf toughness, low nutrient content (wa ...200819161699
erwinia amylovora strains isolated in romania from outbreaks of fire blight disease: phenotypic characterization.the fire blight disease was described for the first time in romania, in 1992. since then by continuous spreading, this disease has caused severe damages of the fruit trees production, particularly of the pear and quince orchards in different regions of the country, being advantaged by certain weather conditions (high temperatures and humidity). an epidemiological surveillance of this disease that was spreading over different regions of the country, has been instituted since 2002. during the year ...200819496475
effect of seven different pollens on bio-ecological parameters of the predatory mite typhlodromus foenilis (acari: phytoseiidae).development, immature survival, reproduction, and life table parameters of the predatory mite typhlodromus foenilis oudemans were evaluated on seven different plant pollens (almond, apple, pear, cherry, apricot, plum, and walnut). experiments were conducted in environmental chambers at 20 +/- 1 degrees c with 65 +/- 5% rh and a photoperiod of 16:8 (l:d). t. foenilis successfully completed its development on all pollen diets. female mean developmental time from egg to adult emergence ranged from ...200818419905
the genome of erwinia tasmaniensis strain et1/99, a non-pathogenic bacterium in the genus erwinia.the complete genome of the bacterium erwinia tasmaniensis strain et1/99 consisting of a 3.9 mb circular chromosome and five plasmids was sequenced. strain et1/99 represents an epiphytic plant bacterium related to erwinia amylovora and e. pyrifoliae, which are responsible for the important plant diseases fire blight and asian pear shoot blight, respectively. strain et1/99 is a non-pathogenic bacterium and is thought to compete with these and other bacteria when occupying the same habitat during i ...200818462403
fermentation of opuntia stricta (haw.) fruits for betalains concentration.fermentation of juice and homogenized fruits of opuntia stricta fruits has been developed and optimized. the aim was to obtain the red food colorant betanin from prickly pear, at high concentration and low viscosity. among three strains assayed, saccharomyces cerevisiae var. bayanus awri 796 has been the optimum for this process. the optimum temperature value was found to be 35 degrees c for both sugar consumption and pigment preservation. after fermentation, biomass and residual vegetal tissue ...200818473472
efficacy of heat treatments with water and fludioxonil for postharvest control of blue and gray molds on inoculated pears and fludioxonil residues in fruit.the residue levels of fludioxonil (flu) were determined in pear cultivars precoce di fiorano, coscia, and spadona estiva after a 2-min dip in an aqueous mixture of flu containing 300 or 100 mg/liter of active ingredient at 20 or 50 degrees c and after 12 days at 17 degrees c and 80% relative humidity (simulated shelf life conditions). the accumulation trend of flu residues was determined in 'precoce di fiorano' pears after treatments with 25, 50, 100, or 200 mg/liter of active ingredient at 20 o ...200818522031
efficacy of dietary supplementation with botanicals on carbohydrate metabolism in humans.botanical products are widely used in nutritional supplementation for promotion of health or prevention of diseases. with the high prevalence of obesity and type 2 diabetes, abnormalities in carbohydrate metabolism are common in the general population and obtaining glycemic control is important in reducing the complications of diabetes. if shown to be effective, botanical products have a unique position in potentially aiding the general public in regard to obesity and diabetes. they can be obtai ...200818537692
sperm morphology and fertility of progeny-tested ai dairy bulls in sweden.use of bull semen with high levels of sperm abnormalities, reflecting genital dysfunction, is not recommended for artificial insemination (ai) since it would most likely lead to subfertility. sperm quality, including sperm morphology, may deteriorate with increasing age of the bull thus becoming a source of concern when using older, progeny-tested ai bull sires. although a relationship between sperm morphology and fertility after ai in progeny-tested bull sires has been reported, it is yet uncle ...200818539320
histological features and management of a mandibular gorham disease: a case report and review of maxillofacial cases in the literature.gorham disease (gd) is a rare osteolysis without sex, race, or age predilection, affecting bones in different regions. based on clinical, histological, and molecular features, diagnosis is difficult and required exclusion of neoplastic, inflammatory, infectious, and endocrinologic disease. etiology is still unknown. we report the case of a 36-year-old man suffering from severe progressive osteolysis located at the mandible. histology showed massive osteolysis without malignant cells. immunohisto ...200818567509
[antitumor effect of polysaccharides from cactus pear fruit in s180-bearing mice].polysaccharide components of some traditional chinese medicine have certain antitumor effects and can promote immune responses. extractions from cactus pear fruit can inhibit the proliferation of cervical cancer, ovary cancer and bladder cancer cells, and suppress the growth of ovarian cancer in mice. this study was to observe the antitumor effect of polysaccharides extracted from cactus pear fruit in s180-bearing mice.200818570729
viability of lactobacillus rhamnosus r0011 in an apple-based fruit juice under simulated storage conditions at the consumer level.lactobacillus rhamnosus r0011 was inoculated in an apple-pear-raspberry juice blend at 4.5 x 10(9) cfu/250 ml portion, and viability was followed during storage in conditions simulating consumer use. study parameters were age of the juice (2 or 4 wk), sampling size (one to three 250-ml portions), incubation temperature (between 2 and 7 degrees c), and atmosphere (aerobic compared with anaerobic). dependent variables were ph, dissolved oxygen (do) level, and viable count. the do level in the unop ...200818577004
control of postharvest pear diseases using rhodotorula glutinis and its effects on postharvest quality parameters.rhodotorula glutinis was evaluated for its activity in reducing postharvest gray mold decay and blue mold decay of pear caused by botrytis cinerea and penicillium expansum respectively, and in reducing natural decay development of pear fruits, as well as its effects on postharvest quality of fruits. there was a significant negative correlation between concentrations of the yeast cells and infectivity of the pathogens. at concentrations of r. glutinis at 5x108 cfu/ml, the gray mold decay was comp ...200818579245
[gorham-stout syndrome in a patient with hiv infection]. 200818582424
four species of zygophiala (schizothyriaceae, capnodiales) are associated with the sooty blotch and flyspeck complex on apple.sooty blotch and flyspeck (sbfs) is a complex of fungi that cause late-season blemishes of apple and pear fruit that cosmetically damage the cuticle, which result in fruit that are unacceptable to consumers. previous studies reported that a single, wide-host-range species, schizothyrium pomi (presumed anamorph zygophiala jamaicensis), caused flyspeck on apple. in the present study we compared morphology and dna phylogeny (its, lsu) of 139 fungal strains isolated from flyspeck signs from 39 apple ...200818592899
macrophage immunomodulatory activity of polysaccharides isolated from opuntia polyacantha.opuntia polyacantha (prickly pear cactus) has been used extensively for its nutritional properties; however, less is known regarding medicinal properties of opuntia tissues. in the present study, we extracted polysaccharides from o. polyacantha and used size-exclusion chromatography to fractionate the crude polysaccharides into four polysaccharide fractions (designated as opuntia polysaccharides c-i to c-iv). the average m(r) of fractions c-i through c-iv was estimated to be 733, 550, 310, and 1 ...200818597716
a rapid and effective method for rna extraction from different tissues of grapevine and other woody plants.rna quality and integrity are critical for many studies in plant molecular biology. high-quality rna extraction from grapevine and other woody plants is problematic due to the presence of polysaccharides, polyphenolics and other compounds that bind or co-precipitate with the rna.200818618437
clinical and molecular delineation of the 17q21.31 microdeletion syndrome.the chromosome 17q21.31 microdeletion syndrome is a novel genomic disorder that has originally been identified using high resolution genome analyses in patients with unexplained mental retardation.200818628315
evaluation of four whole-plant inoculation methods to analyze the pathogenicity of erwinia amylovora under quarantine conditions.four methods were tested to assess the fire-blight disease response on grafted pear plants. the leaves of the plants were inoculated with erwinia amylovora suspensions by pricking with clamps, cutting with scissors, local infiltration, and painting a bacterial suspension onto the leaves with a paintbrush. the effects of the inoculation methods were studied in dose-time-response experiments carried out in climate chambers under quarantine conditions. a modified gompertz model was used to analyze ...200818645961
hrpn of erwinia amylovora functions in the translocation of dspa/e into plant cells.the type iii secretion system (t3ss) is required by plant pathogenic bacteria for the translocation of certain bacterial proteins to the cytoplasm of plant cells or secretion of some proteins to the apoplast. the t3ss of erwinia amylovora, which causes fire blight of pear, apple and other rosaceous plants, secretes dspa/e, which is an indispensable pathogenicity factor. several other proteins, including hrpn, a critical virulence factor, are also secreted by the t3ss. using a cyaa reporter syste ...200818705858
[application of icp-ms to detection of trace elements and heavy metals in different kinds of honey].honey contains abundant trace elements, which are important nutritional elements for human health, but the difference between different species has not been reported. seven kinds of trace elements and five kinds of heavy metals in different honey species were analyzed by the method of icp-ms. the results showed that honey contained many wholesome elements, such as ca, fe, zn, mn, cu, se and mo, but there was great difference between different species. the highest concentrations of ca, fe, zn, mn ...200818800735
an antibacterial pyrazole derivative from burkholderia glumae, a bacterial pathogen of rice.burkholderia glumae, a bacterial pathogen on rice, produced compounds in liquid culture that, in agar diffusion assays, gave strong inhibitory action against erwinia amylovora, the bacterium responsible for fire blight disease of apple and pear trees. products were isolated from culture medium by cation exchange and then purified by bioassay-guided chromatographic methods. two major products were obtained, one of which was not active when fully purified. each product showed a single ninhydrin-st ...200818834606
the c-terminal half of the hrpn virulence protein of the fire blight pathogen erwinia amylovora is essential for its secretion and for its virulence and avirulence activities.the hrpn (harpin) protein of the fire blight pathogen erwinia amylovora is an essential virulence factor secreted via the bacterial type iii secretion system. hrpn also has avirulence activity when delivered to tobacco by e. amylovora and has defense elicitor activity when applied to plants as a cell-free protein extract. here, we characterize a series of random mutations in hrpn that altered the predicted amino acid sequence of the protein. amino acid substitutions and deletions in the highly c ...200818842089
medicinal agents in the metabolic syndrome.the metabolic syndrome (ms) has become a worldwide health problem. it is difficult for patients to follow a diet/exercise regime that would improve their symptoms, therefore the investigation of agents that may deal with its more serious aspects is an important medical field for research. the cardiovascular consequences associated with the syndrome and some of the therapeutic approaches are discussed. the different agents can be divided into several groups: inorganic/ organic: zinc complexes wit ...200818855636
isolation and identification of species of alicyclobacillus from orchard soil in the western cape, south africa.alicyclobacilli were isolated from orchard soil collected from an apple and pear farm in elgin, western cape, south africa. morphological, biochemical and physiological characteristics of the isolates were used to presumptively classify them as belonging to the genus alicyclobacillus. strains were identified to species level by polymerase chain reaction (pcr) with genus-specific primers, and 16s ribosomal rna (rrna) gene sequencing. to our knowledge this is the first report on the isolation of a ...200817938854
lymphatics and bone.there is controversy regarding whether lymphatic vessels are present or absent in bone. although lymphangiomas have been described in bone, lymphatic vessels have not been identified morphologically with certainty in any other benign or malignant bone tumors or in normal human bone. in this study, we determined by immunohistochemistry, using 2 specific lymphatic endothelial cell markers, lyve-1 and podoplanin, whether lymphatics are present in normal bone and a wide range of primary and secondar ...200817904616
effect of chitin on the antagonistic activity of cryptococcus laurentii against penicillium expansum in pear fruit.this study was designed to evaluate the impact of chitin on the antagonistic activity of cryptococcus laurentii against the postharvest blue mold rot caused by penicillium expansum in pear fruit. the results showed that the antagonistic activity of c. laurentii obtained from the culture media of nutrient yeast dextrose broth (nydb) amended with chitin at 0.5-1.0% was improved greatly compared with the case that without chitin. the addition of chitin to nydb did not influence the growth of c. lau ...200818162192
deletion of a 236 kb region around s 4-rnase in a stylar-part mutant s 4sm-haplotype of japanese pear.japanese pear (pyrus pyrifolia nakai) has a gametophytic self-incompatibility (gsi) mechanism controlled by a single s-locus with multiple s-haplotypes, each of which contains separate genes that determine the allelic identity of pistil and pollen. the pistil s gene is the s-ribonuclease (s-rnase) gene, whereas good candidates for the pollen s gene are the f-box protein genes. a self-compatible (sc) cultivar, 'osa-nijisseiki', which is a bud mutant of 'nijisseiki' (s (2) s (4)), has a stylar-par ...200818175198
sperm morphology and chromatin integrity in swedish warmblood stallions and their relationship to pregnancy rates.artificial insemination is not as widely used in horses as in other domestic species, such as dairy cattle and pigs, partly because of the wide variation in sperm quality between stallion ejaculates and partly due to decreased fertility following the use of cooled transported spermatozoa. furthermore, predictive tests for sperm fertilising ability are lacking. the objective of the present study was to assess sperm morphology and chromatin integrity in ejaculates obtained from 11 warmblood breedi ...200818179691
comparative analysis of polyphenolic profiles and antioxidant and antimicrobial activities of tunisian pome fruit pulp and peel aqueous acetone extracts.pome trees, apple, pear, and quince, are classified into the subfamily pomoideae, belonging to the rosaceae family. their autumnal fruits are consumed worldwide in different forms, that is, fresh or transformed into jams, jelly, juices, etc. their well-established beneficial properties to human health were found mainly related to their phenolic content. pulp and peel aqueous acetone extracts obtained from tunisian fruits at commercial maturity were comparatively evaluated for their phenolic prof ...200818181568
efficacy of the pear ester as a monitoring tool for codling moth cydia pomonella (lepidoptera: tortricidae) in new zealand apple orchards.the behavioural response of both sexes of codling moth, cydia pomonella to the pear-derived kairomone (ethyl (2e,4z)-2,4-decadienoate), codling moth sex pheromone (e,e-8,10-dodecadien-1-ol), and sex pheromone combined with the pear derived kairomone loaded into red rubber septum were investigated in trapping experiments in new zealand apple orchards. a range of 0.01-10.0 mg of pheromone loading in rubber septum dispensers was tested and the highest catch of males was in traps baited with 1.0 mg. ...200818189264
efficiency assessment of a swift method to enhance substandard viability ram ejaculates.semen availability in ram semen processing facilities is of great importance for the genetic improvement of sheep. accordingly, any method that would increase sperm viability in low viability ejaculates could be useful. in this study, the possibility of a glass beads filtration method by estimating the beads total surface provided (tsp) for adhesion of spermatozoa, was evaluated. initially, two different tsp (102 and 154 cm(2)) achieved by various sizes of beads (1500, 2000 and 3000 microm) were ...200818199267
role of sertoli cell number and function on regulation of spermatogenesis.testicular function is under the control of expression and repression of several genes and gene products, and many of these works through sertoli cells. the capability of sertoli cells to regulate spermatogenesis is dependent on sertoli cell functions and sertoli cell number. sertoli cell number has long been thought to be stable in adults with no proliferation of sertoli cells once adult numbers have been reached. however, adult horses do not have stable sertoli cell numbers, and new studies in ...200818242891
natural phenanthrenes and their biological activity.the aim of this review is to survey the various naturally occurring phenanthrene compounds that have been isolated from different plants. only one review has previously been published on this topic. gorham (1989) reviewed the structures, biosynthesis, separations and spectroscopy of stilbenes and phenanthrenes. the present study furnishes an overview of the hydroxy or/and methoxy-substituted 9,10-dihydro/phenanthrenes, methylated, prenylated and other monomeric derivatives, dimeric and trimeric ...200818243254
risk assessment of metals from consuming vegetables, fruits and rice grown on soils irrigated with waters of the ebro river in catalonia, spain.the concentrations of arsenic (as), cadmium (cd), chromium (cr), copper (cu), mercury (hg), manganese (mn), nickel (ni), and lead (pb) were determined in samples of various edible vegetables (artichoke, cauliflower, lettuce, tomato), fruits (apple, mandarin, orange, pear) and rice grown in soils irrigated with water from the ebro river in tarragona province (catalonia, spain). although all food items were randomly acquired in various localities of the area, the local origin was always verified. ...200818322656
particle films for managing arthropod pests of apple.a two-season study showed that a calendar-based spray program to manage arthropod pests with kaolin (60 g/liter) applied at the rate of 450 liters/ha was effective against european apple sawfly, hoplocampa testudinea (klug) (hymenoptera: tenthredinidae); white apple leafhopper, typhlocyba pomaria mcatee (homoptera: cicadellidae); apple red bug, lygidea mendax reuter (heteroptera: miridae); pear plant bug, lygocoris communis (knight) (heteroptera: miridae); and the apple rust mite, aculus schlech ...200818330129
feeding substrates and behaviors of western cherry fruit fly (diptera: tephritidae).a study was conducted to determine the abundance of potential foods and the feeding substrates and behaviors of the western cherry fruit fly, rhagoletis indifferens curran (diptera: tephritidae), in 2005, 2006, and 2007 in central washington state. aphid colonies with honeydew, a presumed food source for flies, were not seen on randomly selected branches of sweet cherry trees, prunus avium l., but leaves with cherry juice, fruit that were damaged, and leaves with bird feces were commonly seen, e ...200818348808
measurement of glenoid bone loss: a comparison of measurement error between 45 degrees and 0 degrees bone loss models and with different posterior arthroscopy portal locations.osteotomies at an angle of 45 degrees to the long axis of the glenoid were originally used in a cadaveric model to simulate the bone loss that can occur clinically in anterior instability of the shoulder. however, this type of glenoid defect is not consistent with the usual clinical scenario, in which bone loss occurs nearly parallel (at 0 degrees) to the long axis of the glenoid.200818354146
protective effect of cactus (opuntia ficus indica) cladode extract upon nickel-induced toxicity in rats.the purpose of this study carried out on male wistar rats, was to evaluate the protective effects of regular ingestion of juice from the prickly pear cactus (opuntia ficus indica) cladodes against nickel chloride toxicity. rats were given either normal tap water or water containing 25% of cactus juice for one month. then, rats of each group were injected daily, for 10 days, with either nicl(2) solution (4mg (30micromol)/kg body weight) or with the same volume of saline solution (300mm nacl). sig ...200818950672
duplex real-time polymerase chain reaction reveals competition between erwinia amylovora and e. pyrifoliae on pear blossoms.erwinia amylovora and e. pyrifoliae are the causative agents of fire blight and asian pear blight, respectively. the pathogens are closely related, with overlapping host ranges. data are unavailable on the current distribution of e. pyrifoliae and on the interaction between the two species when they are present together on the same host. in this study, a duplex real-time polymerase chain reaction (pcr) protocol was developed to monitor the population dynamics of e. amylovora and e. pyrifoliae on ...200818944291
ultrastructural study on acibenzolar-s-methyl-induced scab resistance in epidermal pectin layers of japanese pear leaves.the infection behavior of japanese pear scab pathogen venturia nashicola race 1 was studied ultrastructurally in acibenzolar-s-methyl (asm)-pretreated susceptible japanese pear (cv. kousui) leaves to determine the mechanism of asm-induced scab resistance. on asm-pretreated leaf surfaces, the infection behavior (conidial germination and appressorial formation) was similar to that on distilled water (dw)-pretreated leaves prior to cuticle penetration by the pathogen. however, after penetration, di ...200818943227
antibiosis and acidification by pantoea agglomerans strain e325 may contribute to suppression of erwinia amylovora.pantoea agglomerans strain e325, a commercially available antagonist for fire blight of apple and pear, was originally selected through screening based on suppression of erwinia amylovora on flower stigmas, but specific mechanisms of antagonism were unknown. bacterial modification of ph was evaluated as a possible mechanism by analyzing stigma exudates extracted from 'gala' apple stigmas. the ph values for field samples were only slightly lower than controls, but indicated a range (ph 5 to 6) co ...200818943460
patulin causes dna damage leading to cell cycle arrest and apoptosis through modulation of bax, p(53) and p(21/waf1) proteins in skin of mice.patulin (pat), a mycotoxin found in apples, grapes, oranges, pear and peaches, is a potent genotoxic compound. who has highlighted the need for the study of cutaneous toxicity of pat as manual labour is employed during pre and post harvest stages, thereby causing direct exposure to skin. in the present study cutaneous toxicity of pat was evaluated following topical application to swiss albino mice. dermal exposure of pat, to mice for 4 h resulted in a dose (40-160 mug/animal) and time (up to 6 h ...200919000704
in vitro characterisation of koala chlamydia pneumoniae: morphology, inclusion development and doubling time.chlamydia pneumoniae is a common human and animal pathogen associated with upper and lower respiratory tract infections. of the animal c. pneumoniae isolates, the koala nasal isolate (lpcoln) is by far the best genetically characterised. this current study was designed to characterise the morphology and developmental events for the lpcoln isolate, and our results showed several striking in vitro growth differences when compared to the human isolate, ar39. the lpcoln inclusion size and morphology ...200919026498
effect of a waal mutation on lipopolysaccharide composition, oxidative stress survival, and virulence in erwinia amylovora.erwinia amylovora, the causal agent of fire blight, is an enterobacterial pathogen of rosaceous plants including apple and pear. we have been studying the response of e. amylovora to oxidative stress because, during infection, the bacterium elicits an oxidative burst response in host plants. during the screening of a transposon mutant library for hydrogen peroxide sensitivity, we identified a mutant carrying an insertion in waal, a gene involved in lipopolysaccharide biosynthesis, that was more ...200919076232
the luxs gene is involved in ai-2 production, pathogenicity, and some phenotypes in erwinia amylovora.erwinia amylovora causes fire blight of apple, pear, and other members of the rosaceae family. the enzyme luxs catalyzes the last step in the production of autoinducer-2 (ai-2), a molecule implicated with quorum sensing in many bacterial species. it is now well recognized that luxs also plays a central role in sulfur metabolism and in the activated methyl cycle, which is responsible for the generation of s-adenosyl-l-methionine. a research paper has reported that luxs is not involved with quorum ...200918820970
mycobased synthesis of silver nanoparticles and their incorporation into sodium alginate films for vegetable and fruit preservation.biosynthesis of silver nanoparticles using trichoderma viride and their incorporation into sodium alginate for vegetable and fruit preservation has been demonstrated in this study. aqueous silver (ag(+)) ions when exposed to the filtrate of t. viride are reduced in solution. these extremely stable silver nanoparticles were characterized by means of uv-vis spectrophotometer, ftir, tem, and eds. the nanoparticles exhibit maximum absorbance at 421 nm in the uv spectrum. the presence of proteins was ...200919552418
effect of aqueous extracts of alligator pear seed (persea americana mill) on blood glucose and histopathology of pancreas in alloxan-induced diabetic rats.effects of aqueous extract of alligator pear seed on normal and alloxan-induced diabetic rats were investigated in 6 groups of rats (5 rats per group). test groups were made diabetic with intra-peritoneal injection of alloxan and treated with 300 mg and 600 mg/kg body weight of alligator pear seed extract. two non-diabetic groups were also administered with 300 mg and 600 mg/kg body weight extract. the levels of blood glucose were examined in all 6 experimental groups. in diabetic rats, blood gl ...200919553173
fatty acid composition in lipid fractions lengthwise the mycelium of mortierella isabellina and lipid production by solid state fermentation.this paper investigates the correlation between mycelial age and fatty acid biosynthesis. the correlation was investigated by analyzing the lipid composition lengthwise the mycelium of the oleaginous fungus mortierella isabellina, a potential producer of gamma-linolenic acid (gla). young mycelia were rich in polar lipids (glycolipids plus sphingolipids and phospholipids), while neutral lipid content increased in aged mycelia. in young mycelia, each polar lipid fraction contained almost 40% (w/w) ...200919574039
seasonal occurrence and impact of halyomorpha halys (hemiptera: pentatomidae) in tree fruit.halyomorpha halys is an introduced stink bug species from asia that is spreading throughout the mid-atlantic united states. it is native to south korea, japan, and eastern china, where it is an occasional pest of tree fruit, including apple and pear. cage experiments with adults placed on apple and peach during critical plant growth stages demonstrate that it can cause damage to developing fruit during mid- and late season growth periods and that feeding occurs on all regions of the fruit. feedi ...200919610429
light, electron microscopy and histopathology of myxobolus salminus n. sp., a parasite of salminus brasiliensis from the brazilian pantanal.in this report, we describe the morphology and histopathology of myxobolus salminus n. sp., a parasite of the gill filaments of wild salminus brasiliensis (dourado) from the brazilian pantanal. the small polysporic plasmodia were approximately 100 microm in diameter and the development was asynchronous. the mature spores were oval to pear shaped and had a smooth wall. the spore measurements were (mean+/-s.d., with range in parentheses): length 10.1+/-0.4 microm (9.6-10.5), width 6.1+/-0.4 microm ...200919640650
influence of post-harvest treatments with fludioxonil and soy lecithin co-application in controlling blue and grey mould and fludioxonil residues in coscia pears.the residue levels of fludioxonil (flu) were determined in coscia pear following a 1-, 2- or 4-min dip in an aqueous mixture of flu containing 300 or 100 mg l(-1) (active ingredient, a.i.) at 20 and 50 degrees c, respectively, with or without 2% soy lecithin. the efficacy of heat treatment with water and flu mixtures was investigated on artificially inoculated pears for the control of post-harvest decay caused by blue (penicillium expansum link) and grey (botrytis cinerea pers. ex fr.) mould. tr ...200919680873
complex combined vascular malformations and vascular malformation syndromes affecting the extremities in children.complex combined vascular malformations affecting extremities are an interesting group of vascular malformations because, in addition to the vascular channel anomalies present, they can be associated with other tissue changes and sometimes altered limb growth. at present, magnetic resonance imaging is the gold standard imaging tool to evaluate such complex conditions in children because of its inherent tissue specificity and vascular capabilities that enables characterization of tissues and the ...200919724993
description of cryptosporiopsis kienholzii and species profiles of neofabraea in major pome fruit growing districts in the pacific northwest usa.the objectives of this study were i) to give a taxonomic description of a fungus phylogenetically related to neofabraea and assign the name cryptosporiopsis kienholzii to this fungus, ii) to expand previous neofabraea species profiles from infected apple and pear fruit collected from major pome fruit production districts in oregon and washington, and iii) to determine the sensitivity of neofabraea alba, neofabraea malicorticis, neofabraea perennans, and c. kienholzii to a range of fungicides. a ...200919733662
endodontic management of horizontally placed molars after gunshot injury to mandible: a case report.a 37-year-old man reported to our department with the history of gunshot injury to the mandible 15 years before. his anterior mandible had been resected earlier and bone graft was seen. intraoral examination of lower jaw revealed 4 remaining mandibular molars. these teeth were severely rotated such that they lay horizontal with respect to the mandibular base. preoperative dentascan spiral computerized tomography (ct) of the patient revealed obliteration of the mesial canals of the mandibular rig ...200919734072
functional analysis of the n terminus of the erwinia amylovora secreted effector dspa/e reveals features required for secretion, translocation, and binding to the chaperone dspb/f.dspa/e is a type iii secreted effector protein required for pathogenicity in the apple and pear pathogen erwinia amylovora, and dspb/f is a small chaperone protein involved in dspa/e secretion. while the secretion and translocation signals of many type iii secretion effector proteins in human enteric pathogens have been characterized extensively, relatively little is known about the translocation requirements of many effectors in plant pathogens, including large dspe-like proteins. in this study ...200919737101
genotypic comparison of pantoea agglomerans plant and clinical strains.pantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. however, commercial registration of p. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (bl2) organism due to clinical reports as an opportunistic human pathogen. this study compares plant-origin and clinical strains in a search for discriminating genotypic/phenotypic ...200919772624
erwinia amylovora: modern methods for detection and differentiation.erwinia amylovora is the causative agent of fire blight, a very destructive disease of numerous members of the rosaceae. the primary route of infection for host species, including commercially grown apple and pear, is the newly opened blossom. susceptibility of flowers to infection for only a few days creates narrow window for infection. not surprisingly, the risk of disease is related to e. amylovora population size. as a result, methods that supply quick, accurate and sensitive quantification ...200919301751
population structure of potebniamyces pyri in the u.s. pacific northwest and evidence of outcrossing inferred with sequence-characterized amplified region markers.potebniamyces pyri is the cause of phacidiopycnis rot of d'anjou pear, which is grown primarily in washington and oregon. to estimate the population structure of p. pyri, 146 single-spore isolates were sampled from five major pear-production areas and scored for variation at eight sequence-characterized amplified region (scar) loci. significant genetic differentiation was detected among five subpopulations and a total of 54 multilocus genotypes were identified, with significant genotypic diversi ...200919351249
specific detection and quantification of the phytopathogenic agent 'candidatus phytoplasma prunorum'.'candidatus phytoplasma prunorum' is a wall-less bacterium associated with european stone fruit yellows (esfy), a severe disease of prunus spp. (mainly apricot and japanese plum trees). it can be spread by one insect vector, cacopsylla pruni, and by the trade of infected material. the availability of pcr-based methods allowing a sensitive and specific detection of 'ca. p. prunorum' is crucial for this phytoplasma because, at present, it is uncultured and cannot be detected serologically. we deve ...200919401232
a familial case of trichorhinophalangeal syndrome type i.trichorhinophalangeal type i (trps i) is a rare autosomal dominant disorder characterized by variable clinical expression of sparse and slow-growing hair, pear-shaped nose, elongated philtrum, and bone deformities, including cone-shaped epiphyses of the phalanges and short stature. we describe three members of a family who consulted us because of slow-growing scalp hair with craniofacial and radiological features typical of trps i.200919419465
fine structure of the bird parasites trichomonas gallinae and tetratrichomonas gallinarum from cultures.the trophozoites of trichomonas gallinae and tetratrichomonas gallinarum were studied by means of light and electron microscopy after cloning and cultivating them axenically. t. gallinae trophozoites varied in shape reaching from ovoidal to pyriform and had a size of about 7-11 microm. they were provided with four free flagella and a fifth recurrent one, which did not become free at the posterior pole. the nucleus was ovoid, had a size of about 2.5-3 microm, and was situated closely below the ba ...200919421777
first report on the presence of fire blight resistance in linkage group 11 of pyrus ussuriensis maxim.fire blight, caused by the gram-negative bacterium erwinia amylovora (burrill) winslow et al., is a dangerous disease of pome fruits, including pear. a pear breeding program for fire blight resistance was initiated in 2003 at the department of pomology, warsaw university of life sciences, poland. since several asian species are considered to be potential sources of resistance to fire blight, the susceptible pyrus communis 'doyenne du comice' was crossed with the resistant p. ussuriensis. the f1 ...200919433906
stress tolerance and environmental fitness of pseudomonas fluorescens a506, which has a mutation in rpos.establishment of suppressive populations of bacterial biological control agents on aerial plant surfaces is a critical phase in biologically based management of floral diseases. periodically, biocontrol agents encounter inhospitable conditions for growth on plants; consequently, tolerance of environmental stresses may contribute to their fitness. in many gram-negative bacteria, including strains of pseudomonas spp., the capacity to survive environmental stresses is influenced by the stationary p ...200919453226
role of rpos in stress tolerance and environmental fitness of the phyllosphere bacterium pseudomonas fluorescens strain 122.bacteria living epiphytically on aerial plant surfaces encounter severe and rapidly fluctuating environmental conditions, and their capacity to withstand environmental stress contributes to epiphytic fitness. the stationary phase sigma factor rpos is a key determinant in stress response of gram-negative bacteria, including pseudomonas spp. this study focused on the role of rpos in stress response and epiphytic fitness of pseudomonas fluorescens strain 122 on aerial plant surfaces. rpos had a sig ...200919453227
systems level analysis of two-component signal transduction systems in erwinia amylovora: role in virulence, regulation of amylovoran biosynthesis and swarming motility.two-component signal transduction systems (tcsts), consisting of a histidine kinase (hk) and a response regulator (rr), represent a major paradigm for signal transduction in prokaryotes. tcsts play critical roles in sensing and responding to environmental conditions, and in bacterial pathogenesis. most tcsts in erwinia amylovora have either not been identified or have not yet been studied.200919470164
cd105/endoglin expression in gorham disease of bone.gorham disease is a rare pathological condition characterised by a proliferation of vascular channels of haematic and lymphatic origin in bone and adjacent soft tissues, which results in a progressive destruction of the involved bone segment.200919181633
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