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severe hypoglycemia associated with use of trimethoprim/sulfamethoxazole in a patient with chronic renal insufficiency. 200818364403
characterization of two compatible small plasmids from sphingobium yanoikuyae.we isolated and characterized two small cryptic indigenous plasmids, pyan-1 (4,896 bp) and pyan-2 (4,687 bp), from sphingobium yanoikuyae, and developed a versatile system that permitted genetic manipulation of the genus sphingomonas. nucleotide sequencing of both plasmids revealed that they contained moba, mobs, and repa genes, which are predicted to encode proteins associated with mobilization and replication, in common. transformation with each plasmid harboring the antibiotic resistance gene ...200818391447
optimization of gellan gum production by sphingomonas paucimobilis atcc 31461 with nonionic surfactants using central composite design.the effect of nonionic surfactants on gellan production by sphingomonas paucimobilis was studied by the addition of 0.5, 0.75, 1.0, 1.25 and 1.5 g/l surfactants to shake flask culture. the nonionic surfactants tween 80, tween 40 and triton x-100 improved gellan production by s. paucimobilis, and the maximum yield (10.44 g/l) was obtained with triton x-100 at 0.75 g/l compared with that of the control fermentation (8.63 g/l) without surfactant. the do profiles associated with gellan production in ...200818397769
[recognition and analysis on primary metabolites of nonylphenol].in order to discuss the nonylphenol (np) biodegradability effect in the wastewater treatment process, the primary metabolites and route of np had been analyzed in the molecular level by the interpretation of gas chromatography mass spectrometry (gc-ms) and the two experimental methods. a method was that activated sludge degraded two types of simulating sewage comparatively. type-1 wastewater was made up of water, glucose, peptone and inorganic salts, and type-2 sewage was mixed up of np and type ...200818637365
isolation, phylogenetic analysis and screening of marine mollusc-associated bacteria for antimicrobial, hemolytic and surface activities.this study was undertaken to survey culturable heterotrophic bacteria associated with the marine ark shell anadara broughtoni inhabiting in the sea of japan, and to test isolates for their antimicrobial, hemolytic and surface activities with an emphasis on low-molecular-weight metabolites search. a total of 149 strains were isolated and identified phenotypically. a total of 27 strains were selected to be investigated phylogenetically by 165 rrna gene sequence analysis. the most bacteria were aff ...200819216104
[risk assessment for environmental release of genetically engineered microorganism m-cds-1].to assess the environmental release risk of genetically engineered microorganism sphingomonas sp. m-cds-1.200819149163
substrate recognition by family 7 alginate lyase from sphingomonas sp. a1.sphingomonas sp. a1 alginate lyase a1-ii', a member of polysaccharide lyase family 7, shows a broad substrate specificity acting on poly alpha-l-guluronate (poly(g)), poly beta-d-mannuronate (poly(m)) and the heteropolymer (poly(mg)) in alginate molecules. a1-ii' with a glove-like beta-sandwich as a basic scaffold forms a cleft covered with two lid loops (l1 and l2). here, we demonstrate the loop flexibility for substrate binding and structural determinants for broad substrate recognition and ca ...200818514736
interactions among buffelgrass, phenanthrene and phenanthrene-degrading bacteria in gnotobiotic microcosms.an experiment was undertaken in gnotobiotic microcosms to determine the role of buffelgrass (cenchrus ciliaris) and a phenanthrene-degrading bacterium (strain pm600) in the degradation of phenanthrene. the gram-negative bacterium was identified as a sphingomonas sp. by 16s rrna gene sequence analysis and as s. paucimobilis by biochemical tests (api 20 ne strips). its yellow pigment corresponded to nostoxanthin and its cellular fatty acids were typical of the genus sphingomonas. moreover, it was ...200818569317
cloning and characterization of phosphoglucomutase and phosphomannomutase derived from sphingomonas chungbukensis dj77.the enzymes phosphoglucomutase (pgm) and phosphomannomutase (pmm) play an important role in the synthesis of extracellular polysaccharide. by colony hybridization of the fosmid library of sphingomonas chungbukensis dj77, an open reading frame (orf-1) of 1,626 nucleotides, whose predicted product is highly homologous with other pgm proteins from several bacterial species, was identified. an additional open reading frame (orf-2) of 1,437 nucleotides was identified, and its encoded protein shows a ...200818664520
molecular characterization of the bacterial communities in the different compartments of a full-scale reverse-osmosis water purification plant.the origin, structure, and composition of biofilms in various compartments of an industrial full-scale reverse-osmosis (ro) membrane water purification plant were analyzed by molecular biological methods. samples were taken when the ro installation suffered from a substantial pressure drop and decreased production. the bacterial community of the ro membrane biofilm was clearly different from the bacterial community present at other locations in the ro plant, indicating the development of a speci ...200818621875
degradation behaviors of nonylphenol ethoxylates by isolated bacteria using improved isolation method.nonylphenol ethoxylate (npeo)-degrading bacteria were isolated from activated sludge using an improved isolation method, and the corresponding degradation behaviours were investigated. eight npeo-degrading strains distributed in genera pseudomonas, sphingomonas, sphingobium, cupriavidus, ralstonia, achromobacter, and staphylococcus were acquired. the latter five genera have never been reported for the degradation of npeos. four degradation patterns were observed for the eight pure strains. in pa ...200819143306
factors influencing the electrokinetic dispersion of pah-degrading bacteria in a laboratory model aquifer.despite growing interest in the electro-bioremediation of contaminated soil it is still largely unknown to which degree weak electric fields influence the fate of contaminant-degrading microorganisms in the sub-surface. here we evaluate the factors influencing the electrokinetic transport and deposition of fluorene-degrading sphingomonas sp. lb126 in a laboratory model aquifer exposed to a direct current (dc) electric field (1 v cm(-1)) typically used in electro-bioremediation measures. the infl ...200818607588
[variation of bacterial community composition in the outbreak and decline of microcystis spp. bloom in lake xuanwu].water samples were collected in the outbreak and decline of microcystis spp. bloom in three different lake areas of lake xuanwu for studying the bacterial community composition during the bloom to provide clew for understanding the dynamics of water bloom. traditional microbiological methods were used for culturable bacterial analysis, while the amplified products of variable v3 region of bacterial 16s rdna were used for bacterial community dna finger print by using denaturing gradient gel elect ...200819143401
acclimated biomass that degrades sulfonated naphthalene formaldehyde condensate.a number of aerobic species were isolated from textile industry activated sludge wastewater. the bacterial consortium was acclimated during seven days before testing its capacity of sulfonated naphthalene-formaldehyde condensate (snfc) recalcitrant compound degradation. snfc's degradation was evaluated by using different techniques including: vapour pressure osmometry, spectroscopy uv-visible and chemical oxygen demand (cod). the degradation of snfc by acclimated bacterial consortium was determi ...200818819646
isomer-specific biodegradation of methylphenanthrenes by soil bacteria.it is assumed that bacteria generally degrade 2-methylphenanthrene (2mphe) in preference to 1-methylphenanthrene (1mphe), and that environmental biodegradation of methylated pahs therefore can be described qualitatively by changes in relative concentrations of these isomers. our objective was to investigate whether microbial phenanthrene degraders (sphingomonas and mycobacterium) show such isomer-specific pah degradation. eleven out of twenty-nine phenanthrene degraders could grow on methylphena ...200818678007
enrichment and characterization of chlorinated organophosphate ester-degrading mixed bacterial cultures.chlorinated organophosphate ester (ope)-degrading enrichment cultures were obtained using tris(2-chloroethyl) phosphate (tcep) or tris(1,3-dichloro-2-propyl) phosphate (tdcpp) as the sole phosphorus source. in cultures with 46 environmental samples, significant tcep and tdcpp degradation was observed in 10 and 3 cultures, respectively, and successive subcultivation markedly increased their degradation rates. 67e and 45d stable enrichment cultures obtained with tcep and tdcpp, respectively, compl ...200818691527
protein engineering of improved prolyl endopeptidases for celiac sprue therapy.due to their unique ability to cleave immunotoxic gluten peptides endoproteolytically, prolyl endopeptidases (peps) are attractive oral therapeutic candidates for protecting celiac sprue patients from the toxic effects of dietary gluten. enhancing the activity and stability of peps under gastric conditions (low ph, high pepsin concentration) is a challenge for protein engineers. using a combination of sequence- and structure-based approaches together with machine learning algorithms, we have ide ...200818836204
sphingomonas paucimobilis associated with localised calf myositis. 200818796597
a putative lipoprotein of sphingomonas sp. strain a1 binds alginate rather than a lipid moiety.gram-negative sphingomonas sp. strain a1 accumulates alginate in the cell surface pit and directly incorporates the polysaccharide into its cytoplasm through a 'superchannel'. a cell surface protein algp7 (27 kda) is inducibly expressed in the presence of alginate. although the protein algp7 was initially classified as a lipoprotein based on its primary structure, algp7 purified from strain a1 cells did not possess a lipid moiety. algp7 bound alginate efficiently at a neutral ph with a k(d) of 3 ...200818803672
[relationship between soil fungistasis and bacterial community structure].as a natural attribute of clean and healthy soil, fungistasis is an important indicator of soil quality, and has positive ecological significance to the inhibition of plant disease eruption caused by soil-borne fungal pathogens. in this study, soil samples (0-15 cm) were collected from the abandoned land at shenyang experimental station of ecology, chinese academy of sciences, to which pesticides and fertilizers have never been applied for nearly 10 years. a series of soil samples with gradient ...200818839921
regulatory roles for nkt cell ligands in environmentally induced autoimmunity.the development of autoimmune diseases is frequently linked to exposure to environmental factors such as chemicals, drugs, or infections. in the experimental model of metal-induced autoimmunity, administration of subtoxic doses of mercury (a common environmental pollutant) to genetically susceptible mice induces an autoimmune syndrome with rapid anti-nucleolar ab production and immune system activation. regulatory components of the innate immune system such as nkt cells and tlrs can also modulat ...200818981095
treatment of pahs in contaminated soil by extraction with aqueous dna followed by biodegradation with a pure culture of sphingomonas sp.the biodegradation of polycyclic aromatic hydrocarbons (pahs) in aqueous deoxyribonucleic acid (dna) solution from contaminated soil washing was investigated. initial data with a model effluent consisting of anthracene, phenanthrene, pyrene and benzo[a]pyrene that were individually dissolved in 1% aqueous dna solution confirmed their positive degradation by sphingomonas sp. at around 10(8)cfuml(-1) initial cell loading. for anthracene and phenanthrene, complete removal was achieved within 1h tre ...200818814900
[a rare cause of nosocomial bacteremia: sphingomonas paucimobilis].sphingomonas paucimobilis, is a yellow-pigmented, aerobic, non-fermentative, non-spore-forming, gram-negative bacillus. infections by s. paucimobilis which is widely found in nature and hospital environments are rarely serious or life threatening. in this report we present a case of hospital acquired bloodstream infection due to s. paucimobilis. the patient had a history of hydrocephalus diagnosed at sixth months of his birth and had experienced two ventriculoperitoneal shunt surgery. he was hos ...200819149092
degradation of diphenyl phthalate by sphingomonas chungbukensis.more than 80% of diphenyl phthalate (dpp) at 100 mg l(-1) was degraded by sphingomonas chungbukensis kctc 2955 in a mineral salts medium at ph 7.0 and 30 degrees c within 48 h. the maximum specific degradation rate was 5 mg dpp l(-1 )h(-1). it was rapidly converted to monophenyl phthalate and phthalic acid which were further degraded.200817673949
interaction of higher plant (jute), electrofused bacteria and mycorrhiza on anthracene biodegradation.the interaction of bacteria, mycorrhiza and jute (corchotus capsulari, a higher plant) to reduce anthracene in different concentrations of spiked soils was investigated. dominant indigenous bacterium (pseudomonas sp.) isolated in the rhizosphere of jute was electrofused with anthracene degraders (sphingomonas paucimobilis and pseudomonas aeruginosa) which were able to produce different types of biosurfactants. the highest population (56 x 10(5)cfu/g) was found in the planted soil with the inocul ...200817662599
benzo[a]pyrene degradation by sphingomonas yanoikuyae jar02.batch experiments were conducted to characterize the degradation of benzo[a]pyrene, a representative high molecular weight (hmw) polycyclic aromatic hydrocarbon (pah), by sphingomonas yanoikuyae jar02. concentrations up to the solubility limit (1.2 microg l(-1)) of benzo[a]pyrene were completely removed from solution within 20 h when the bacterium was grown on salicylate. additional experiments with [(14)c]7-benzo[a]pyrene demonstrated 3.8% mineralization over 7 days when salicylate was present ...200817482734
different bacterial groups for biodegradation of three- and four-ring pahs isolated from a hong kong mangrove sediment.mangrove sediments have been found to degrade three- to four-ring pahs extensively. in the present study, 11 strains from 4 genera mycobacterium (3 strains), sphingomonas (5), terrabacter (2) and rhodococcus (1) were isolated from a single surface sediment sample of a hong kong mangrove swamp, among which the terrabacter strains were isolated to grow with fluoranthene for the first time. although all four genera could degrade three- and four-ring pahs, their in situ activities in natural sedimen ...200817868985
hypothesized role of galactocerebroside and nkt cells in the etiology of multiple sclerosis.according to the molecular mimicry theory, multiple sclerosis (ms) develops when the immune system mistakenly attacks a component of the myelin sheath that is structurally similar to a foreign epitope. the glycolipid galactocerebroside (galc) is a major component of myelin. as lipids comprise between 70% and 85% of myelin, glycolipids should be investigated as candidate autoantigens in ms. galc displays broad structural similarities to the borrelia burgdorferi glycolipid antigen bbgl-2 and to th ...200817889444
quantitative structure-activity relationships for kinetic parameters of polycyclic aromatic hydrocarbon biotransformation.quantitative structure-activity relationships (qsars) were developed for three monod-type parameters--qmax, ks, and qmax/ks--that express the kinetics of polycyclic aromatic hydrocarbon (pah) biotransformation by sphingomonas paucimobilis strain epa505. the training sets contained high-quality experimental values of the kinetic parameters for 20 unsubstituted and methylated pahs as well as values of 41 meaningful molecular descriptors. a genetic function approximation algorithm was used to devel ...200818366261
effects of the inoculant strain sphingomonas paucimobilis 20006fa on soil bacterial community and biodegradation in phenanthrene-contaminated soil.the effects of the inoculant strain sphingomonas paucimobilis 20006fa (isolated from a phenanthrene-contaminated soil) on the dynamics and structure of microbial communities and phenanthrene elimination rate were studied in soil microcosms artificially contaminated with phenanthrene. the inoculant managed to be established from the first inoculation as it was evidenced by denaturing gradient gel electrophoresis analysis, increasing the number of cultivable heterotrophic and pah-degrading cells a ...200817694405
phylogenetic diversity of nitrogen-fixing bacteria in mangrove sediments assessed by pcr-denaturing gradient gel electrophoresis.culture-independent pcr-denaturing gradient gel electrophoresis (dgge) was employed to assess the composition of diazotroph species from the sediments of three mangrove ecosystem sites in sanya, hainan island, china. a strategy of removing humic acids prior to dna extraction was conducted, then total community dna was extracted using the soil dna kit successfully for nifh pcr amplification, which simplified the current procedure and resulted in good dgge profiles. the results revealed a novel ni ...200818347779
second step of hydrolytic dehalogenation in haloalkane dehalogenase investigated by qm/mm methods.mechanistic studies on the hydrolytic dehalogenation catalyzed by haloalkane dehalogenases are of importance for environmental and industrial applications. here, car-parrinello (cp) and oniom hybrid quantum-mechanical/molecular mechanics (qm/mm) are used investigate the second reaction step of the catalytic cycle, which comprises a general base-catalyzed hydrolysis of an ester intermediate (ei) to alcohol and free enzyme. we focus on the enzyme linb from sphingomonas paucimobilis ut26, for which ...200817729274
pentachlorophenol contaminated groundwater bioremediation using immobilized sphingomonas cells inoculation in the bioreactor system.pentachlorophenol (pcp) has been used as a wood preservative for more than 100 years. the extensive use of pcp has widely contaminated soil and groundwater. pcp is toxic to living organisms. the main objective of this research was to inoculate the pure pcp-degrading bacterium strain sphingomonas chlorophenolica pcp-1, isolated from pcp-contaminated soils, into pcp-contaminated groundwater for remediation purposes. the factors that influenced the bioremediation were explored with batch experiment ...200817686581
isolation and characterization of phenanthrene-degrading strains sphingomonas sp. zp1 and tistrella sp. zp5.two bacteria strains sphingomonas sp. strain zp1 and tistrella sp. strain zp5 were identified as phenanthrene-degrading ones, based on gram staining, oxydase reaction, biochemical tests, fame analysis, g+c content and 16s rdna gene sequence analysis. we isolated these two bacteria strains sphingomonas sp. zp1 and tistrella sp. zp5 from soil samples contaminated with polycyclic aromatic hydrocarbon (pah)-containing waste from oil refinery field in shanghai, china. strain sphingomonas sp. zp1 was ...200817850962
extracellular polysaccharides produced by cooling water tower biofilm bacteria and their possible degradation.the extracellular polymers (eps) of biofilm bacteria that can cause heat and mass transfer problems in cooling water towers in the petrochemical industry were investigated. in addition, these microorganisms were screened for their ability to grow and degrade their own eps and the eps of other species. twelve bacteria producing the most eps were isolated from cooling water towers and characterized biochemically by classic and commercial systems. these were species of pseudomonas, burkholderia, ae ...200818256966
characterization of a novel angular dioxygenase from fluorene-degrading sphingomonas sp. strain lb126.in this study, the genes involved in the initial attack on fluorene by sphingomonas sp. strain lb126 were investigated. the alpha and beta subunits of a dioxygenase complex (flna1-flna2), showing 63 and 51% sequence identity, respectively, to the subunits of an angular dioxygenase from the gram-positive dibenzofuran degrader terrabacter sp. strain dbf63, were identified. when overexpressed in escherichia coli, flna1-flna2 was responsible for the angular oxidation of fluorene, 9-hydroxyfluorene, ...200818156320
biodegradation potential and bacterial diversity of a petrochemical wastewater treatment plant in iran.an activated sludge treatment was evaluated for its effectiveness in cleaning up a petrochemical wastewater in iran. for assessing biodegradation potential of activated sludge, seven characteristics of wastewater (temperature, ph, dissolved oxygen, chemical oxygen demand, concentrations of ethylene dichloride, vinyl chloride, and total hydrocarbons) were monitored during six months. it was shown that dominant pollutants in order of magnitude were normal-alkanes (c(10)-c(21)), aromatics, and poly ...200818255283
intergeneric coaggregation among drinking water bacteria: evidence of a role for acinetobacter calcoaceticus as a bridging bacterium.intergeneric coaggregation of drinking water bacteria was tested. acinetobacter calcoaceticus was found not only to autoaggregate but also to coaggregate with four of the five other isolates (burkholderia cepacia, methylobacterium sp., mycobacterium mucogenicum, sphingomonas capsulata, and staphylococcus sp.). in its absence, no coaggregation was found. interactions were lectin-saccharide mediated. the putative bridging function of a. calcoaceticus was evidenced by multispecies biofilm studies, ...200818156333
decolorization of anthraquinone dye intermediate and its accelerating effect on reduction of azo acid dyes by sphingomonas xenophaga in anaerobic-aerobic process.decolorization of 1-aminoanthraquinone-2-sulfonic acid (asa-2) and its accelerating effect on the reduction of azo acid dyes by sphingomonas xenophaga qyy were investigated. the study showed that asa-2 could be efficiently decolorized by strain qyy under aerobic conditions according to the analysis of total organic carbon removal and uv-vis spectra changes. moreover, strain qyy was able to reduce azo acid dyes under anaerobic conditions. the effects of various operating conditions such as carbon ...200818074231
isolation of hexachlorocyclohexane-degrading sphingomonas sp. by dehalogenase assay and characterization of genes involved in gamma-hch degradation.to screen and identify bacteria from contaminated soil samples which can degrade hexachlorocyclohexane (hch)-isomers based on dechlorinase enzyme activity and characterize genes and metabolites.200818042212
microbial degradation of chlorinated dioxins.polychlorinated dibenzo-p-dioxins (pcdd) and polychlorinated dibenzofurans (pcdf) were introduced into the biosphere on a large scale as by-products from the manufacture of chlorinated phenols and the incineration of wastes. due to their high toxicity they have been the subject of great public and scientific scrutiny. the evidence in the literature suggests that pcdd/f compounds are subject to biodegradation in the environment as part of the natural chlorine cycle. lower chlorinated dioxins can ...200818083210
crystal structure of a novel bacterial cell-surface flagellin binding to a polysaccharide.bacterial flagellins are generally self-assembled into extracellular flagella for cell motility. however, the flagellin homologue p5 is found on the cell surface of sphingomonas sp. a1 (strain a1) and binds tightly to the alginate polysaccharide. to assimilate alginate, strain a1 forms a mouthlike pit on the cell surface and concentrates the polymer in the pit. p5 is a candidate receptor that recognizes extracellular alginate and controls pit formation. to improve our understanding of the struct ...200818189419
metabolic degradation of phenazine-1-carboxylic acid by the strain sphingomonas sp. dp58: the identification of two metabolites.the biotransformation of phenazine-1-carboxylic acid (pca) by pca-degrading strain sphingomonas sp. dp58 yielded small quantities of metabolites and was demonstrated for the first time. the metabolites were isolated by using preparative high-performance liquid chromatography (hplc). in addition, these were subsequently characterized by gas chromatography (gc)-mass spectrum (ms) after n,o-bis(trimethylsilyl)trifluoroacetamide (bstfa) derivatization and (1)h-nuclear magnetic resonance (nmr). they ...200818097645
discovery of a bacterial gene cluster for catabolism of the plant hormone indole 3-acetic acid.the isolation and annotation of an 8994-bp dna fragment from pseudomonas putida 1290, which conferred upon p. putida kt2440 the ability to utilize the plant hormone indole 3-acetic acid (iaa) as a sole source of carbon and energy, is described. this iac locus (for indole 3-acetic acid catabolism) was identified through analysis of a plasposon mutant of p. putida 1290 that was no longer able to grow on iaa or indole 3-acetaldehyde and was unable to protect radish roots from stunting by exogenousl ...200818205812
bacterial diversity in synovial fluids of patients with tmd determined by cloning and sequencing analysis of the 16s ribosomal rna gene.the purpose of this study was to examine the presence of bacteria in synovial fluids from patients with temporomandibular joint disorder (tmd) by polymerase chain reaction (pcr) targeting the 16s ribosomal rna (rrna) gene, followed by cloning and sequencing.200818206402
effect of rhamnolipids on degradation of anthracene by two newly isolated strains, sphingomonas sp. 12a and pseudomonas sp. 12b.anthracene is a pah that is not readily degraded, plus its degradation mechanism is still not clear. thus, two strains of bacteria-degrading bacteria were isolated from longterm petroleum-polluted soil and identified as sphingomonas sp. 12a and pseudomonas sp. 12b by a 16s rrna sequence analysis. to further enhance the anthracene-degrading ability of the two strains, the biosurfactants produced by pseudomonas aeruginosa w3 were used, which were characterized as rhamnolipids. it was found that th ...200818239418
biodegradation kinetics of select polycyclic aromatic hydrocarbon (pah) mixtures by sphingomonas paucimobilis epa505.many contaminated sites commonly have complex mixtures of polycyclic aromatic hydrocarbons (pahs) whose individual microbial biodegradation may be altered in mixtures. biodegradation kinetics for fluorene, naphthalene, 1,5-dimethylnaphthalene and 1-methylfluorene were evaluated in sole substrate, binary and ternary systems using sphingomonas paucimobilis epa505. the first order rate constants for fluorene, naphthalene, 1,5-dimethylnaphthalene, and 1-methylfluorene were comparable; yet monod para ...200817534722
organization of three rrna (rrn) operons from sphingobium chungbukense dj77.the nucleotide sequences of all three rrna operons (rrna, rrnb, and rrnc) of sphingobium chungbukense dj77 were determined. the three rrn operons have the same gene order (16s rrna-trnaile-trnaala-23s rrna-5s rrna-trnafmet). the nucleotide sequences were identical over a 5,468 bp region spanning the 16s rrna gene to the 5s rrna gene. variability was observed in the 5s rrna-trnafmet spacer sequence of rrnb. the trnafmet gene sequences were identical except for two bases (t5794 and a5871 in rrnb, ...200819107400
[degradation characteristics of bromoamine acid by sphingomonas sp. fl].a bacterial strain that could degrade bromoamine acid (baa) as the sole carbon source was isolated. it was identified as sphingomonas sp. based on 16s rrna gene sequence analysis and physio-biochemical characteristics. under the optimal growth conditions, with temperature of 30 degrees c, ph of 7.0, rotating rate of 100 r/min and (nh4)2so4 as the nitrogen source, the decolorization percentage of baa (100 mg/l) could reach 99% within 14 h. nacl of low concentration ( < 2%) facilitated the decolor ...200819068653
arbuscular mycorrhiza and petroleum-degrading microorganisms enhance phytoremediation of petroleum-contaminated soil.while plants can phytoremediate soils that are contaminated with petroleum hydrocarbons, adding microbes to remediate contaminated sites with petroleum-degrading microorganisms and arbuscular mycorrhizal fungi (amf) is not well understood. the phytoremediation of arabian medium crude oil (aco) was done with a lolium multiflorum system inoculated with an amf (glomus intraradices) and a mixture of petroleum-degrading microorganisms--the bacterium, sphingomonas paucimobilis (sp) and the filamentous ...200819260211
rhizoremediation of lindane by root-colonizing sphingomonas.we used a two-step enrichment approach to isolate root-colonizing hexachlorocyclohexane (hch)-degrading microorganisms. the first step consists of the use of classical liquid enrichment to isolate γ-hch degraders. the γ-hch-degrading microbes were attached in mass to corn seeds sown in soil with γ-hch, and after plant development we rescued bacteria growing on root tips. bacteria were then subjected to a second enrichment round in which growth on liquid medium with γ-hch and inoculation of corn ...200821261825
activity and viability of polycyclic aromatic hydrocarbon-degrading sphingomonas sp. lb126 in a dc-electrical field typical for electrobioremediation measures.there has been growing interest in employing electro-bioremediation, a hybrid technology of bioremediation and electrokinetics for the treatment of contaminated soil. knowledge however on the effect of weak electrokinetic conditions on the activity and viability of pollutant-degrading microorganisms is scarce. here we present data about the influence of direct current (dc) on the membrane integrity, adenosine triphosphate (atp) pools, physico-chemical cell surface properties, degradation kinetic ...200821261821
physiological traits of the symbiotic bacterium teredinibacter turnerae isolated from the mangrove shipworm neoteredo reynei.nutrition in the teredinidae family of wood-boring mollusks is sustained by cellulolytic/nitrogen fixing symbiotic bacteria of the teredinibacter clade. the mangrove teredinidae neoteredo reynei is popularly used in the treatment of infectious diseases in the north of brazil. in the present work, the symbionts of n. reynei, which are strictly confined to the host's gills, were conclusively identified as teredinibacter turnerae. symbiont variants obtained in vitro were able to grow using casein a ...200921637522
molecular and biochemical characterization of the tetralin degradation pathway in rhodococcus sp. strain tfb.the tetralin biodegradation pathway in rhodococcus sp. strain tfb, a gram-positive bacterium resistant to genetic manipulation, was characterized using a proteomic approach. relative protein expression in cell free extracts from tetralin- and glucose-grown cells was compared using the 2d-dige technique. identification of proteins specifically expressed in tetralin-grown cells was used to characterize a complete set of genes involved in tetralin degradation by reverse genetics. we propose a tetra ...200921261920
isolation and characterization of novel bisphenol-a--degrading bacteria from soils.one hundred and seven soil samples were collected from various places in japan, and their bisphenol-a (bpa, 2,2-bis(4-hydroxyphenyl) propane) degradative capacities were evaluated. eighty-five soil samples possessed bpa degradative capacities, and 26 bacterial strains could be isolated as bpa-degrading bacterium. sequence analysis of their 16s rrna genes indicated that 22 isolates belonged to proteobacteria groups, and three of four gram-positive bacterial strains, ya27, no13, and no15, were cla ...200920055221
random transposon vectors puttns for the markerless integration of exogenous genes into gram-negative eubacteria chromosomes.a set of random transposon vectors puttns that facilitates the markerless integration of new functions into the chromosome of gram-negative bacteria has been developed. the vectors, which are derived from mini-tn5 transposons, are located on a r6k-based suicide delivery plasmid that provides the is50(r) transposase tnp gene in cis, but they are external to the mobile element. the vectors' conjugal transfer to recipients is mediated by rp4 mobilization functions in the donor. internal to the mini ...200919778558
sorption to humic acids enhances polycyclic aromatic hydrocarbon biodegradation.the hypothesis that humic acids (ha) can act as carriers of polycyclic aromatic hydrocarbon (pah) compounds and provide bacteria with pah fluxes beyond those obtained by diffusion of the water-dissolved fraction of these poorly soluble chemicals was tested. controlled degradation experiments using microcrystalline phenanthrene and sphingomonas sp. lh162 were performed without ha and in the presence of two ha concentrations. in the absence of ha, phenanthrene was steadily present at the maximum d ...200919848123
cloning and characterization of phosphomannose isomerase from sphingomonas chungbukensis dj77.phosphomannose isomerase (pmi) catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate in the extracellular polysaccharide (eps) synthesis pathway. the gene encoding pmi in sphingomonas chungbukensis dj77 was cloned and expressed in e. coli. the pmi gene is 1,410 nucleotides long and the deduced amino acid sequence shares high homology with other bifunctional proteins that possess both pmi and gdp-mannose pyrophosphorylase (gmp) activities. the sequence analysis of pmi reve ...200919712590
the microorganism community of pentachlorophenol (pcp)-degrading coupled granules.coupled granules are self-immobilized aggregates of microorganisms under micro aerobic conditions, which have the dissolved oxygen (do) level of 0.6 mg.l(-1). the effects of do concentration on pentachlorophenol (pcp) reduction and its microbial community were investigated in a coupled anaerobic and aerobic reactor. both the diversity and the dynamicity of the eubacteria and archaea community, which were responsible for pcp degradation, were evaluated by means of amplification by polymerase chai ...200919273898
biodegradation of polycyclic aromatic hydrocarbons by sphingomonas sp. enhanced by water-extractable organic matter from manure compost.the effectiveness of in-situ bioremediation of polycyclic aromatic hydrocarbons (pahs) may be inhibited by their low aqueous solubility and strong absorption to soil constituents. the aim of this research was to evaluate the effect of water-extractable organic matter (weom) from manure compost on the biodegradation of various pahs. the aqueous solubilities of pahs including phenanthrene, pyrene and benzo[a]pyrene under different concentrations of weom from cow manure compost were initially evalu ...200919660784
top-down systems biology modeling of host metabotype-microbiome associations in obese rodents.covariation in the structural composition of the gut microbiome and the spectroscopically derived metabolic phenotype (metabotype) of a rodent model for obesity were investigated using a range of multivariate statistical tools. urine and plasma samples from three strains of 10-week-old male zucker rats (obese (fa/fa, n=8), lean (fa/-, n=8) and lean (-/-, n=8)) were characterized via high-resolution 1h nmr spectroscopy, and in parallel, the fecal microbial composition was investigated using fluor ...200919275195
asymmetric dihydroxylation of aryl olefins by sequential enantioselective epoxidation and regioselective hydrolysis with tandem biocatalysts.chiral aryl vicinal diols were obtained in high ee and yield by asymmetric dihydroxylation of aryl olefins with tandem biocatalysts: one contains an enantioselective styrene monooxygenase, and the other contains a regioselective epoxide hydrolase.200919277363
[sphingomonas sp.: an important microbial resource for biopolymer synthesis].the genus sphingomonas was established in 1990. sphingomonas spp. synthesize sphingans, structurally related biopolymers such as gellan, welan and diutan. at present, only gellan is applied widely in foods and pharmaceuticals. the economic value of other sphingans has not been well explored, and related research of sphingans still remains limited. in the present review, we address the latest taxonomy developments of sphingomonas, details about structure, characteristics and biosynthetic pathway ...200919637560
crystallization and preliminary crystallographic analysis of the cell-surface alginate-binding protein algp7 isolated from sphingomonas sp. a1.sphingomonas sp. a1, a gram-negative bacterium, directly internalizes alginate macromolecules through a mouth-like pit that is present on its cell surface. the alginate-binding protein algp7, which was found to be localized on the cell surface, contributes to the accumulation of alginate in the pit. algp7 was crystallized at 293 k by means of the sitting-drop vapour-diffusion method with polyethylene glycol 3350 as a crystallizing agent. preliminary x-ray analysis showed that the algp7 crystal b ...200919407391
diversity, abundance, and consistency of microbial oxygenase expression and biodegradation in a shallow contaminated aquifer.the diversity of rieske dioxygenase genes and short-term temporal variability in the abundance of two selected dioxygenase gene sequences were examined in a naphthalene-rich, coal tar waste-contaminated subsurface study site. using a previously published pcr-based approach (s. m. ní chadhain, r. s. norman, k. v. pesce, j. j. kukor, and g. j. zylstra, appl. environ. microbiol. 72:4078-4087, 2006) a broad suite of genes was detected, ranging from dioxygenase sequences associated with rhodococcus a ...200919700556
analysis of genes encoding the 2,4-dichlorophenoxyacetic acid-degrading enzyme from sphingomonas agrestis 58-1.a 2,4-dichlorophenoxy acetic acid (2,4-d)-degrading bacterium, strain 58-1, was newly isolated from soil samples collected in the fukuoka prefecture, japan, and grown on an enrichment culture medium containing 2,4-d as the sole carbon source. phylogenic analysis identified strain 58-1 as sphingomonas agrestis. in 2,4-d degraders, classes i, ii, and iii inherit the tfda, cada, and tfdaalpha genes, respectively, and the results from degenerate-pcr indicated that this strain belongs to the class ii ...200919577193
association between contaminated faucets and colonization or infection by nonfermenting gram-negative bacteria in intensive care units in taiwan.this study was designed to determine the strength of the association between the isolation of nonfermentative gram-negative bacilli (nfgnb) from tap water faucet aerators and the prevalence of colonization or infection of patients in intensive care units (icus). surveillance cultures were obtained during a 4-month period from 162 faucet aerators located in seven different icus. the prevalence of colonization or infection of icu patients with nfgnb was determined by prospective surveillance durin ...200919587299
novel marine carbazole-degrading bacteria.eleven carbazole (car)-degrading bacterial strains were isolated from seawater collected off the coast of japan using two different media. seven isolates were shown to be most closely related to the genera erythrobacter, hyphomonas, sphingosinicella, caulobacter, and lysobacter. meanwhile, strains oc3, oc6s, oc9, and oc11s showed low similarity to known bacteria, the closest relative being kordiimonas gwangyangensis gw14-5 (90% similarity). southern hybridization analysis revealed that only five ...200919187209
[effect of the inoculant strain sphingomonas paucimobilis 20006fa on the bacterial composition of a phenanthrene-degrading consortium].the effect of the inoculant strain sphingomonas paucimobilis 20006fa on the bacterial composition of a phenanthrene-degrading consortium obtained from a pristine soil in sequencing batch cultures was studied. inoculated (f200+1) and non-inoculated (f200) phenanthrene-degrading consortia, were obtained. bacterial diversity of consortia was studied at cultivable (phenotype and genotype characterization) and non-cultivable (pcr-dgge) levels. during the successive cultures, a loss in the phenanthren ...200919623894
sphingomonas hankookensis sp. nov., isolated from wastewater.a gram-negative, non-motile, rod-shaped bacterial strain, odn7(t), was isolated from a wastewater treatment plant in korea, and its taxonomic position was investigated by use of a polyphasic taxonomic approach. strain odn7(t) grew optimally at around ph 7.5 and 30 degrees c and in the presence of 0-0.5 % (w/v) nacl. phylogenetic analysis based on 16s rrna gene sequences showed that strain odn7(t) fell within the cluster comprising species of the genus sphingomonas, clustering with sphingomonas p ...200919625422
water-borne sphingomonas paucimobilis epidemic in an intensive care unit. 200919232740
coaggregation by the freshwater bacterium sphingomonas natatoria alters dual-species biofilm formation.coaggregation is hypothesized to enhance freshwater biofilm development. to investigate this hypothesis, the ability of the coaggregating bacterium sphingomonas natatoria to form single- and dual-species biofilms was studied and compared to that of a naturally occurring spontaneous coaggregation-deficient variant. attachment assays using metabolically inactive cells were performed using epifluorescence and confocal laser scanning microscopy. under static and flowing conditions, coaggregating s. ...200919376917
characterization of a ring-hydroxylating dioxygenase from phenanthrene-degrading sphingomonas sp. strain lh128 able to oxidize benz[a]anthracene.sphingomonas sp. strain lh128 was isolated from a polycyclic aromatic hydrocarbon (pah)-contaminated soil using phenanthrene as the sole source of carbon and energy. a dioxygenase complex, phna1fa2f, encoding the alpha and beta subunit of a terminal dioxygenase responsible for the initial attack on pahs, was identified and isolated from this strain. phna1f showed 98%, 78%, and 78% identity to the alpha subunit of pah dioxygenase from novosphingobium aromaticivorans strain f199, sphingomonas sp. ...200919172265
alpha-glycosphingolipids via chelation-induced anomerization of o- and s-glucuronic and galacturonic acid derivatives.bacterial glycolipids containing either alpha-glucuronic acid or alpha-galacturonic acid residues have an important role in the innate-type immune response to gram-negative bacteria. synthesis of closely related compounds, including a novel alpha-so(2) glycolipid mimetic, is described from carbohydrate precursors where anomerization is a key step. very high stereoselectivites (>97:3 in favor of alpha) were observed from o-glycoside precursors.200919178158
high yield synthesis of 12-aminolauric acid by "enzymatic transcrystallization" of omega-laurolactam using omega-laurolactam hydrolase from acidovorax sp. t31.the genes encoding omega-laurolactam hydrolases from cupriavidus sp. t7, acidovorax sp. t31, cupriavidus sp. u124, and sphingomonas sp. u238 were cloned and sequenced. nucleotide and amino acid sequence analysis of the four genes indicated that the primary structures of these omega-laurolactam hydrolases are significantly similar to the 6-aminohexanoate-cyclic-dimer hydrolase (ec 3.5.2.12). these genes were expressed in escherichia coli, and the omega-laurolactam hydrolysing activity of the reco ...200919420719
[cellulose-utilising bacterial association]. 200919449742
azo dye decolorization by a new fungal isolate, penicillium sp. qq and fungal-bacterial cocultures.a new azo dyes-decolorizing fungi strain qq was isolated from activated sludge. it was identified as penicillium sp. based on 26s rrna gene sequence analysis. the study indicated that strain qq could aerobically decolorize reactive brilliant red x-3b by the way of bioadsorption, and nutrient-poor medium was more beneficial for adsorption. decolorization rate was inversely proportional to the size of mycelial pellets. the optimum ph was observed at 4 or 5 for x-3b decolorization. there was still ...200919473759
impact of bio-augmentation with sphingomonas sp. strain ttnp3 in membrane bioreactors degrading nonylphenol.this study evaluates the potential of bio-augmentation to improve the degradation of recalcitrant nonylphenol during the wastewater treatment in membrane bioreactors (mbr). one mbr containing activated sludge was bio-augmented using multistep inoculation with freeze dried sphingomonas sp. strain ttnp3, whereas a second control reactor contained activated sludge solely. the (14)c-labeled-nonylphenol isomer (4-[1-ethyl-1,3-dimethylpentyl]phenol) was applied as a single pulse. bio-augmentation resu ...200919495744
sphingomonas aestuarii sp. nov., isolated from tidal flat sediment.a novel bacterium (strain k4(t)) belonging to the genus sphingomonas was isolated from tidal flat sediment in korea. its morphology, physiology, biochemical features and 16s rrna gene sequence were characterized. colonies of this strain are yellow in colour and the cells are rod-shaped, exhibiting negative gram staining. the strain grows at 0-5 % (w/v) nacl and 20-35 degrees c, with optimal growth occurring at 0 % (w/v) nacl and 30 degrees c. phylogenetic analysis based on 16s rrna gene sequence ...200919502316
previously uncultured beta-proteobacteria dominate in biologically active granular activated carbon (bac) filters.bacteria colonizing bac filters used in drinking water purification from lake water were characterized by morphology, physiological tests, whole cell protein profiles and plfa (phospholipid fatty acid) composition, and identified by partial 16s rrna gene sequencing. epifluorescence revealed prothecate bacteria to dominate in bac. the majority of the isolates belonged to order burkholderiales of beta-proteobacteria, a few to comamonadaceae but the majority to an undescribed family and the related ...200919783028
quinone-mediated decolorization of sulfonated azo dyes by cells and cell extracts from sphingomonas xenophaga.the effects of various quinone compounds on the decolorization rates of sulfonated azo dyes by sphingomonas xenophaga qyy were investigated. the results showed that anthraquinone-2-sulfonate (aqs) was the most effective redox mediator and aqs reduction was the rate-limited step of aqs-mediated decolorization of sulfonated azo dyes. based on aqs biological toxicity tests, it was assumed that aqs might enter the cells and kill them. in the cytoplasmic extracts from strain qyy, aqs more effectively ...200919634426
potentiation of murine innate immunity by alpha-galacturonosyl-type glycosphingolipids isolated from sphingomonas yanoikuyae and s. terrae.glycosphingolipids (gsls) are components of the outer membrane of sphingomonas species, commonly classified into two types, alpha-glucuronosyl ceramide (alpha-glcacer) and alpha-galacturonosyl ceramide (alpha-galacer), respectively. gsl-7 from s. yanoikuyae and gsl-13 from s. terrae, with alpha-galacer-type structure, possess dihydrosphingosine but with a different ratio of c21cyclopropane to c20:1, while other parts remain similar. we therefore examined if this difference in the ratio of c21cyc ...200919555191
astaxanthin dirhamnoside, a new astaxanthin derivative produced by a radio-tolerant bacterium, sphingomonas astaxanthinifaciens. 200919557030
detection of uvbr-sensitive and -tolerant bacteria in surface waters of the western north pacific.in order to evaluate the effects of solar ultraviolet radiation (uvr) on eubacterial community composition, we examined the tolerance of eubacterial phylotypes to solar uv radiation in surface waters of the western north pacific during september 2005. bromodeoxyuridine (brdu), a halogenated thymine analogue, was used for labeling newly synthesized dna in proliferating cells. thymine dimers (td), which are specifically formed in dna by biologically harmful ultraviolet b radiation (uvbr; 280-315nm ...200919285879
the most-probable-number enumeration of dichlobenil and 2,6-dichlorobenzamide (bam) degrading microbes in finnish aquifers.in groundwater subsurface deposits and a topsoil from five aquifers having 2,6-dichlorobenzamide (bam) in water, we determined the most-probable-number (mpn) of 2,6-dichlorobenzonitrile (dichlobenil) and metabolite bam degrading microorganisms. dichlobenil and bam were combined nitrogen sources in the mpn tubes, which were scored positive at concentrations <75% after 1 month incubation. aerobic and anaerobic microbes degrading dichlobenil and bam were common in samples in low numbers of 3.6-210 ...200919326247
sphingomonas sanxanigenens sp. nov., isolated from soil.strain nx02(t), a gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from soil, and its taxonomic position was investigated using a polyphasic approach. chemotaxonomic analysis revealed that strain nx02(t) possessed q-10 as the predominant ubiquinone, sym-homospermidine as the major polyamine and c(18 : 1)omega7c, c(16 : 0) and c(14 : 0) 2-oh as the major fatty acids. the main polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol ...200919329595
[genetic diversity of cultivable bacteria of dominant plants in a potassium mine tailing of nanjing].plant rhizosphere bacteria play an important role in biogeochemical cycles. microbial diversity of cultivable bacteria from soil-plant-mineral system in a mine tailing was assessed.200919873749
cloning and characterization of phosphoglucose isomerase from sphingomonas chungbukensis dj77.phosphoglucose isomerase (pgi) is involved in synthesizing extracellular polysaccharide (eps). the gene encoding pgi in sphingomonas chungbukensis dj77 was cloned and expressed in e. coli, and the protein was characterized. the pgi gene from dj77 is 1,503 nucleotides long with 62% gc content and the deduced amino acid sequence shows strong homology with pgis from other sources. the molecular masses of pgi subunit and native form were estimated to be 50 kda and 97 kda, respectively. four potentia ...200919336005
vertical partitioning and expression of primary metabolic genes in a thermophilic microbial mat.a thermophilic microbial mat with a relatively simple morphological composition was used to study the expression of key metabolic genes between mat layers. mats comprised roseiflexus castenholzii, synechococcus sp., a sphingomonas-like proteobacterial taxon and an unidentified member of the thermotogae as determined by 16s rrna phylotypes. the diversity of expressed loci for key genes involved in oxygenic photosynthesis (cbbl), anoxygenic photosynthesis (pufm) and nitrogen fixation (nifh) was as ...200919347567
crystal structure of the glycosidase family 73 peptidoglycan hydrolase flgj.glycoside hydrolase (gh) categorized into family 73 plays an important role in degrading bacterial cell wall peptidoglycan. the flagellar protein flgj contains n- and c-terminal domains responsible for flagellar rod assembly and peptidoglycan hydrolysis, respectively. a member of family gh-73, the c-terminal domain (sph1045-c) of flgj from sphingomonas sp. strain a1 was expressed in escherichia coli, purified, and characterized. sph1045-c exhibited bacterial cell lytic activity most efficiently ...200919351587
crystallization and preliminary x-ray crystallographic analysis of phok, an extracellular alkaline phosphatase from sphingomonas sp. bsar-1.alkaline phosphatases (aps) are widely distributed from microbes to humans and are involved in several important biological processes such as phosphate nutrition, signal transduction and pathogenesis. alkaline phosphatases are also useful in various industrial applications and in recombinant dna technology. a new ap enzyme from sphingomonas sp. strain bsar-1, termed phok, has been shown to be useful in uranium bioprecipitation. phok was expressed, purified and crystallized. the crystals belonged ...200919724132
vertical distribution and anaerobic biodegradation of polycyclic aromatic hydrocarbons in mangrove sediments in hong kong, south china.the vertical distribution of polycyclic aromatic hydrocarbons (pahs) at different sediment depths, namely 0-2 cm, 2-4 cm, 4-6 cm, 6-10 cm, 10-15 cm and 15-20 cm, in one of the most contaminated mangrove swamps, ma wan, hong kong was investigated. it was the first time to study the intrinsic potential of deep sediment to biodegrade pahs under anaerobic conditions and the abundance of electron acceptors in sediment for anaerobic degradation. results showed that the total pahs concentrations (summa ...200919683792
circular dichroism spectra, optical rotations and absolute configurations of cis-dihydrodiol metabolites of quinoline and derivatives: the role of the nitrogen atom.the absolute configurations of cis-dihydrodiol bacterial metabolites derived from quinoline substrates and their quinolinium salts have been determined by comparison of experimental and calculated chiroptical data (circular dichroism spectra and optical rotations). the role of intramolecular h-bonding and other interactions in the preferred conformations has been investigated, including the effect of protonation of several cis-dihydrodiols. the presence of a strong intramolecular oh...n bond in ...200919750495
static and dynamic sorption of phenanthrene in mangrove sediment slurry.the static and dynamic sorption of phenanthrene (phe) in three types of mangrove sediment slurries (sandy, silty and muddy) were described by three models, namely linear model, freundlich adsorption isotherm model and langmuir adsorption isotherm model. the freundlich adsorption isotherm was the best model to describe the static sorption behavior of phe in mangrove sediment slurry with the regression coefficients ranging from 0.96 to 0.99. in static sorption, the sorption capacity and sorption i ...200919356852
the external aldimine form of serine palmitoyltransferase: structural, kinetic, and spectroscopic analysis of the wild-type enzyme and hsan1 mutant mimics.sphingolipid biosynthesis begins with the condensation of l-serine and palmitoyl-coa catalyzed by the plp-dependent enzyme serine palmitoyltransferase (spt). mutations in human spt cause hereditary sensory autonomic neuropathy type 1, a disease characterized by loss of feeling in extremities and severe pain. the human enzyme is a membrane-bound hetereodimer, and the most common mutations are located in the enzymatically incompetent monomer, suggesting a "dominant" or regulatory effect. the molec ...200919376777
sphingomonas japonica sp. nov., isolated from the marine crustacean paralithodes camtschatica.a sphingomonas-like bacterium, strain kc7(t), was isolated from a marine crustacean specimen obtained from the sea of japan and subjected to a polyphasic study. comparative 16s rrna gene sequence analysis positioned the novel strain in the genus sphingomonas as an independent lineage adjacent to a subclade containing sphingomonas trueperi lmg 2142(t), sphingomonas pituitosa ediv(t) and sphingomonas azotifigens nbrc 15497(t). strain kc7(t) shared highest 16s rrna gene sequence similarity (96.1 %) ...200919406815
genetic diversity of gamma-hexachlorocyclohexane-degrading sphingomonads isolated from a single experimental field.to isolate gamma-hexachlorocyclohexane (gamma-hch)-degrading bacteria from a single field and to examine their genetic diversity.200919674290
co-ordinated regulation of two divergent promoters through higher-order complex formation by the lysr-type regulator thnr.the genes required for tetralin biodegradation by sphingomonas macrogolitabida strain tfa are clustered in two divergent and closely linked operons. thnr, a lysr-type regulator, activates transcription from each operon in response to tetralin. the regulatory thnr gene is co-transcribed with the catabolic genes thnc, thna3 and thna4, resulting in positive autoregulation. thnr binds with different affinity to two primary binding sites, designated b and c, in the intervening region between the two ...200919682246
mutational study of the role of n-terminal amino acid residues in tetrachlorohydroquinone reductive dehalogenase from sphingomonas sp. ug30.this research presents the first extensive mutational study of n-terminal amino acids necessary for activity of a bacterial zeta class glutathione transferase (gst). our studies on ug30 tetrachlorohydroquinone reductive dehalogenase (pcpc) revealed that, similar to other zeta class gsts, n-terminal ser and cys residues play critical roles in glutathione binding and their mutation results in functional and structural changes to pcpc. mutation of the n-terminal ser and cys residues decreased the a ...200919682569
community proteogenomics reveals insights into the physiology of phyllosphere bacteria.aerial plant surfaces represent the largest biological interface on earth and provide essential services as sites of carbon dioxide fixation, molecular oxygen release, and primary biomass production. rather than existing as axenic organisms, plants are colonized by microorganisms that affect both their health and growth. to gain insight into the physiology of phyllosphere bacteria under in situ conditions, we performed a culture-independent analysis of the microbiota associated with leaves of so ...200919805315
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