Publications

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antifeedant and phytotoxic activity of cacalolides and eremophilanolides.the antifeedant effect of six cacalolides and six eremophilanolides was tested against the herbivorous insects spodoptera littoralis, leptinotarsa decemlineata, and myzus persicae. the test compounds included several natural products isolated from senecio madagascariensis (14-isovaleryloxy-1,2-dehydrocacalol methyl ether, 4), s. barba-johannis (13-hydroxy-14-oxocacalohastine, 5; 13-acetyloxy-14-oxocacalohastine, 6) and s. toluccanus [6-hydroxyeuryopsin, 7; 1(10)-epoxy-6-hydroxyeuryopsin, 9; tolu ...201717708441
establishing alighting preferences and species transmission differences for pea seed-borne mosaic virus aphid vectors.pea seed-borne mosaic virus (psbmv) infection causes a serious disease of field pea (pisum sativum) crops worldwide. the psbmv transmission efficiencies of five aphid species previously found landing in south-west australian pea crops in which psbmv was spreading were studied. with plants of susceptible pea cv. kaspa, the transmission efficiencies of aphis craccivora, myzus persicae, acyrthosiphon kondoi and rhopalosiphum padi were 27%, 26%, 6% and 3%, respectively. lipaphis erysimi did not tran ...201728408208
effects of a genetically modified potato on a non-target aphid are outweighed by cultivar differences.insect-plant interactions may be unintentionally affected when introducing genetically modified (gm) crops into an agro-ecosystem. our aim was to test the non-target effects of a late blight-resistant gm potato on myzus persicae in greenhouse and climate room experiments and understand how position and number of r gene insertions can affect non-targets in gm events. we also aimed to compare results to baseline differences among three conventional potato varieties varying in resistance to late bl ...201728572750
sequential acquisition of potato virus y strains by myzus persicae favors the transmission of the emerging recombinant strains.in the past decade recombinant strains of potato virus y (pvy) have overtaken the ordinary strain, pvy(o), as the predominant viruses affecting the us seed potato crop. aphids may be a contributing factor in the emergence of the recombinant strains, but studies indicate that differences in transmission efficiency of individual pvy strains either from single or mixed infections, although variable, are not generally significant. multiple strains of pvy are present in all potato production areas an ...201728666897
effects of aphidius gifuensis on the feeding behavior and potato virus y transmission ability of myzus persicae.the stylet penetration behavior of aphids when feeding on plants is associated with virus acquisition and inoculation. aphidius gifuensis (ashmead) is a primary endoparasitoid of myzus persicae (sulzer) which is the most efficient vector of plant viruses. information about the effects of parasitoid on aphid and virus transmission can provide an essential foundation for designing effective biological control strategies. this study aimed to investigate the effects of a. gifuensis on the feeding be ...201728485823
corrigendum: the green peach aphid myzus persicae perform better on pre-infested chinese cabbage brassica pekinensis by enhancing host plant nutritional quality. 201728276455
influence of arbuscular mycorrhizal stage and plant age on the performance of a generalist aphid.host plant quality for herbivores is modulated by different factors including symbiosis with soil organisms, such as arbuscular mycorrhiza (am), as well as plant age. however, the role of the developmental stage of the am in such plant-microbe-herbivore interactions has been neglected. to investigate the effects of am stage and plant age on aphid performance, individuals of the generalist myzus persicae were reared on leaves of non-mycorrhized (nm) or mycorrhized (am) plantago lanceolata plants ...201728159616
sublethal effects of thiamethoxam on the demographic parameters of myzus persicae (hemiptera: aphididae).the green peach aphid, myzus persicae sulzer (hemiptera: aphididae), is an important sap-sucking pest of many crops, including chinese cabbage, brassinca oleracea l. the neonicotinoid insecticide thiamethoxam has been used as an effective insecticide to control m. persicae in cabbage fields. in this study, we assessed the effects of sublethal concentrations of thiamethoxam on demographic parameters of m. persicae. in leaf-dip bioassays, thiamethoxam showed a relatively high toxicity against m. p ...201728520891
fear of predation alters clone-specific performance in phloem-feeding prey.fear of predation has been shown to affect prey fitness and behaviour, however, to date little is known about the underlying genetics of responses to predator-associated risk. in an effort to fill this gap we exposed four naïve clones of green peach aphid (myzus persicae), maintained on the model crop brassica oleracea, to different types of cues from aphid lion (chrysoperla carnea). the respective predation risks, we termed fear factors, were either lethal (consumption by predator), or non-leth ...201728794462
early biotic stress detection in tomato (solanum lycopersicum) by bvoc emissions.we investigated impacts of early and mild biotic stress on biogenic volatile organic compounds (bvoc) emissions from tomato in order to test their potential for early (biotic) stress detection. tomato plants were exposed to two common fungal pathogens, botrytis cinerea and oidium neolycopesici and the sap-sucking aphid myzus persicae. furthermore, plants were exposed to methyl jasmonate (meja) in order to identify bvoc emissions related to activation of jasmonic acid (ja) signalling pathway. the ...201728946050
plant insects and mites uptake double-stranded rna upon its exogenous application on tomato leaves.exogenously applied double-stranded rna (dsrna) molecules onto tomato leaves, moved rapidly from local to systemic leaves and were uptaken by agricultural pests namely aphids, whiteflies and mites. four small interfering rnas, deriving from the applied dsrna, were molecularly detected in plants, aphids and mites but not in whiteflies. double-stranded rna (dsrna) acts as the elicitor molecule of the rna silencing (rna interference, rnai), the endogenous and evolutionary conserved surveillance sys ...201728924923
genome of russian wheat aphid an economically important cereal aphid.although the hemipterans (aphididae) are comprised of roughly 50,000 extant insect species, only four have sequenced genomes that are publically available, namely acyrthosiphon pisum (pea aphid), rhodnius prolixus (kissing bug), myzus persicae (green peach aphid) and diuraphis noxia (russian wheat aphid). as a significant proportion of agricultural pests are phloem feeding aphids, it is crucial for sustained global food security that a greater understanding of the genomic and molecular functioni ...201729299110
microrna regulation in an ancient obligate endosymbiosis.although many insects are associated with obligate bacterial endosymbionts, the mechanisms by which these host/endosymbiont associations are regulated remain mysterious. while micrornas (mirnas) have been recently identified as regulators of host/microbe interactions, including host/pathogen and host/facultative endosymbiont interactions, the role mirnas may play in mediating host/obligate endosymbiont interactions is virtually unknown. here, we identified conserved mirnas that potentially media ...201729271121
diversity and evolution of mariner-like elements in aphid genomes.although transposons have been identified in almost all organisms, genome-wide information on mariner elements in aphididae remains unknown. genomes of acyrthosiphon pisum, diuraphis noxia and myzus persicae belonging to the macrosiphini tribe, actually available in databases, have been investigated.201728662628
fasting alters aphid probing behaviour but does not universally increase the transmission rate of non-circulative viruses.a fasting period prior to non-circulative virus acquisition has been shown to increase the rate of transmission by aphids. however, this effect has only been studied for a few virus-vector combinations, and there are contradictory results in the literature as to the role of fasting on virus acquisition. we analysed the influence of fasting on the transmission of three non-circulative viruses, cucumber mosaic virus, zucchini yellow mosaic virus and cauliflower mosaic virus, by two aphid vector sp ...201729134940
systemic deterrence of aphid probing and feeding by novel β-damascone analogues.β-damascone appeared a weak attractant close to not active to myzus persicae, but modifications of its structure caused the avoidance of treated leaves by aphids during settling and reluctance to probe in simple choice- and no-choice experiments in previous studies. here, the electrical penetration graph (epg) technique, which allows monitoring of aphid probing within plant tissues, was applied to explore the biological background and localisation in plant tissues of the deterrent activities of ...201726300715
evaluation of the reference genes for expression analysis using quantitative real-time polymerase chain reaction in the green peach aphid, myzus persicae.the green peach aphid, myzus persicae sulzer (hemiptera, aphididae), is an important cosmopolitan pest. real time qrt-pcr has been used for target gene expression analysis on m. persicae. using real time qrt-pcr, the expression levels are normalized on the basis of the reliable reference genes. however, to date, the stability of available reference genes has been insufficient. in this study, we evaluated nine candidate reference genes from m. persicae under diverse experimental conditions. the t ...201726749166
afit: a bioinformatic tool for measuring aphid fitness and invasiveness.a careful measure of fitness represents a crucial target in crop pest management and becomes fundamental considering extremely prolific insects. in the present paper, we describe a standardized rearing protocol and a bioinformatics tool to calculate aphid fitness indices and invasiveness starting from life table data. we tested the protocol and the bioinformatic tool using six myzus persicae (sulzer) asexual lineages in order to investigate if karyotype rearrangements and ecotype could influence ...201727871340
the interactions of piperonyl butoxide and analogues with the metabolic enzymes fe4 and cyp6cy3 of the green peach aphid myzus persicae (hemiptera: aphididae).piperonyl butoxide (pbo) is a well-known insecticide synergist capable of interacting with phase 1 metabolic enzymes, specifically esterases and cytochrome p450s. in this study, structure-activity relationship analyses were used to characterise the interaction of around 30 analogues of pbo with the esterase fe4 and the p450 cyp6cy3 from insecticide-resistant myzus persicae (sulzer), in order to predict the synthesis of more potent inhibitors.201727174317
rapid transcriptional plasticity of duplicated gene clusters enables a clonally reproducing aphid to colonise diverse plant species.the prevailing paradigm of host-parasite evolution is that arms races lead to increasing specialisation via genetic adaptation. insect herbivores are no exception and the majority have evolved to colonise a small number of closely related host species. remarkably, the green peach aphid, myzus persicae, colonises plant species across 40 families and single m. persicae clonal lineages can colonise distantly related plants. this remarkable ability makes m. persicae a highly destructive pest of many ...201728190401
evolution of imidacloprid resistance in myzus persicae in greece and susceptibility data for spirotetramat.myzus persicae s.l. is a major crop pest globally and has evolved resistance to a range of insecticide classes making it increasingly difficult to control in some areas. here we compare bioassay monitoring data for two important compounds, imidacloprid and spirotetramat, on field samples/clones collected in greece.201728139069
an aphid effector targets trafficking protein vps52 in a host-specific manner to promote virulence.plant- and animal-feeding insects secrete saliva inside their hosts, containing effectors, which may promote nutrient release and suppress immunity. although for plant pathogenic microbes it is well established that effectors target host proteins to modulate host cell processes and promote disease, the host cell targets of herbivorous insects remain elusive. here, we show that the existing plant pathogenic microbe effector paradigm can be extended to herbivorous insects in that effector-target i ...201728100451
self- and conspecific discrimination between unparasitized and parasitized green peach aphid (hemiptera: aphididae), by aphelinus asychis (hymenoptera: aphelinidae).aphelinus asychis walker (hymenoptera: aphelinidae) is a solitary koinobiont endoparasitoid, which parasitizes the green peach aphid, myzus persicae (sulzer) (hemiptera: aphididae). host discrimination is the ability of a parasitoid to distinguish between unparasitized and parasitized hosts, so parasitoids can avoid wasting eggs and time. we determined the influence of host density (5, 10, 15, 20, and 25 aphids per experienced parasitoid during a 24-h period) on egg distribution, and tested the ...201728062793
using vital dyes to trace uptake of dsrna by green peach aphid allows effective assessment of target gene knockdown.rna interference (rnai) is an effective tool to study gene function. for in vitro studies of rnai in insects, microinjection of double-stranded (ds)rna may cause stress. non-persuasive oral delivery of dsrna to trigger rnai is a better mode of delivery for delicate insects such as aphids because it mimics natural feeding. however, when insects feed ad libitum, some individuals may not feed. for accurate measurement of gene knockdown, analysis should only include insects that have ingested dsrna. ...201728054949
discovery of metabolic resistance to neonicotinoids in green peach aphids (myzus persicae) in australia.myzus persicae is a serious pest that attacks a broad range of agricultural crops. this species has developed chemical resistance to many insecticides globally, and within australia resistance to multiple chemical groups has been identified. resistance to neonicotinoid insecticides has been discovered in several countries, but has not previously been confirmed in australia. we use biomolecular assays and bioassays on field-collected populations to investigate neonicotinoid resistance in m. persi ...201727888606
insect deterrent and nematicidal screening of microfungi from mexico and anti-aphid compounds from gliomastix masseei.fungal metabolites are promising alternatives for the development of biorational pesticides. in this sense, microfungi from tropical regions are valuable sources of natural compounds for pest management. with the aim of broadening the search for new eco-friendly products to manage plant pests, this study was carried out to evaluate the biological activity of 23 tropical fungal extracts on three species of phytophagous insects and a plant parasitic nematode. in addition, the active principles of ...201728258772
the cuticle protein gene mpcp4 of myzus persicae (homoptera: aphididae) plays a critical role in cucumber mosaic virus acquisition.myzus persicae (sulzer) (homoptera: aphididae) is one of the most important agricultural pests worldwide. in addition to sucking phloem sap, m. persicae also transmits cucumber mosaic virus (cmv) as a vector in a nonpersistent manner. at present, the infection mechanism remains unclear, especially the process of aphid virus acquisition. in this study, we isolated four m. persicae cuticle protein genes (mpcp1, mpcp2, mpcp4, and mpcp5) from m. persicae. the relative amount of the gene encoding cuc ...201728334092
host plants indirectly influence plant virus transmission by altering gut cysteine protease activity of aphid vectors.the green peach aphid, myzus persicae, is a vector of the potato leafroll virus (plrv, luteoviridae), transmitted exclusively by aphids in a circulative manner. plrv transmission efficiency was significantly reduced when a clonal lineage of m. persicae was reared on turnip as compared with the weed physalis, and this was a transient effect caused by a host-switch response. a trend of higher plrv titer in physalis-reared aphids as compared with turnip-reared aphids was observed at 24 h and 72 h a ...201727932519
whole genome sequence of the soybean aphid, aphis glycines.aphids are emerging as model organisms for both basic and applied research. of the 5,000 estimated species, only three aphids have published whole genome sequences: the pea aphid acyrthosiphon pisum, the russian wheat aphid, diuraphis noxia, and the green peach aphid, myzus persicae. we present the whole genome sequence of a fourth aphid, the soybean aphid (aphis glycines), which is an extreme specialist and an important invasive pest of soybean (glycine max). the availability of genomic resourc ...201728119199
mechanisms underlying the nonconsumptive effects of parasitoid wasps on aphids.natural enemies need not consume herbivores to suppress herbivore populations. behavioral interactions can adversely impact herbivore fitness from reduced time feeding, investment in defense, or injury from failed attacks. the importance of such "nonconsumptive effects" for herbivore suppression may vary across species based on the specificity and intensity of the herbivore defensive response. in a series of manipulative studies, we quantified the nature and consequences of nonconsumptive intera ...201728025222
characterization of an insect heterodimeric voltage-gated sodium channel with unique alternative splicing mode.recent discovery of the heterodimeric voltage-gated sodium channels (nav) in two aphid species, acyrthosiphon pisum and myzus persicae, aroused interest in exploring whether this kind of channel is conserved for aphids. herewith, we aim to provide evidence for the conservation of heterodimeric navs in aphids and investigate whether they have unique splicing patterns. we found that the only identifiable nav from toxoptera citricida consisted of two subunits, forming a heterodimeric nav, which car ...201727816572
sequential above- and belowground herbivory modifies plant responses depending on herbivore identity.herbivore-induced changes in plant traits can cause indirect interactions between spatially and/or temporally separated herbivores that share the same host plant. feeding modes of the herbivores is one of the major factors that influence the outcome of such interactions. here, we tested whether the effects of transient aboveground herbivory for seven days by herbivores of different feeding guilds on tomato plants (solanum lycopersicum) alters their interaction with spatially as well as temporall ...201728178961
synthesis and biological activity of pyridazine amides, hydrazones and hydrazides.optimization studies on compounds initially designed to be herbicides led to the discovery of a series of [6-(3-pyridyl)pyridazin-3-yl]amides exhibiting aphicidal properties. systematic modifications of the amide moiety as well as the pyridine and pyridazine rings were carried out to determine if these changes could improve insecticidal potency.201727766752
synthesis and biological activity of a new class of insecticides: the n-(5-aryl-1,3,4-thiadiazol-2-yl)amides.optimization studies on a high-throughput screening (hts) hit led to the discovery of a series of n-(6-arylpyridazin-3-yl)amides with insecticidal activity. it was hypothesized that the isosteric replacement of the pyridazine ring with a 1,3,4-thiadiazole ring could lead to more potent biological activity and/or a broader sap-feeding pest spectrum. the resulting n-(5-aryl-1,3,4-thiadiazol-2-yl)amides were explored as a new class of insecticides.201727414747
studies toward understanding the sar around the sulfoximine moiety of the sap-feeding insecticide sulfoxaflor.the discovery of sulfoxaflor (isoclast™ active) stemmed from a novel scaffold-based approach toward identifying bioactive molecules. it exhibits broad-spectrum control of many sap-feeding insect pests, including aphids, whiteflies, hoppers and lygus. systematic modifications of the substituents flanking each side of the sulfoximine moiety were carried out to determine whether these changes would improve potency.201727367042
potato virus y transmission efficiency from potato infected with single or multiple virus strains.there has been a recent shift in the prevalence of potato virus y (pvy) strains affecting potato with the ordinary strain pvy(o) declining and the recombinant strains pvy(ntn) and pvy(n:o) emerging in the united states. multiple pvy strains are commonly found in potato fields and even in individual plants. factors contributing to the emergence of the recombinant strains are not well defined but differential aphid transmission of strains from single and mixed infections may play a role. we found ...201727938241
lack of cross-resistance between neonicotinoids and sulfoxaflor in field strains of q-biotype of whitefly, bemisia tabaci, from eastern china.control of bemisia tabaci has depended primarily and heavily on insecticides, especially neonicotinoids. the novel sulfoximine insecticide sulfoxaflor exhibits high potency against a broad range of sap-feeding insect species, including those resistant to neonicotinoids. the resistance levels of q-biotype b. tabaci field strains collected from 8 locations in eastern china to neonicotinoids and sulfoxaflor were investigated, and single nucleotide polymorphisms (snps) of nicotinic acetylcholine rec ...201728187830
insecticidal activity of novel thioureas and isothioureas.we hypothesized that the exploration of chemical space around compounds with reported insecticidal activity could be a viable strategy for discovering novel, insecticidally active areas of chemistry.201727391046
pro-insecticidal approach towards increasing in planta activity.the adrenergic mode of action was investigated for the development of potential new insecticides. clonidine-related analogs were tested against myzus persicae (sulzer) and bemisia tabaci (gennadius). clonidine analogs lack translation owing to a possible vacuole-trapping mechanism. physical property modulation via a prodrug approach was attempted to overcome this mechanism.201727414472
brassica aphid (hemiptera: aphididae) populations are conditioned by climatic variables and parasitism level: a study case of triângulo mineiro, brazil.cosmopolitan pests such as brevicoryne brassicae, lipaphis pseudobrassicae, and myzus persicae (aphididae) cause significant damage to brassicaceae crops. assessment of the important biotic and abiotic factors that regulate these pests is an essential step in the development of effective integrated pest management programs for these aphids. this study evaluated the influence of leaf position, precipitation, temperature, and parasitism on populations of l. pseudobrassicae, m. persicae, and b. bra ...201728316286
biocidal compounds from mentha sp. essential oils and their structure-activity relationships.essential oils from greek mentha species showed different chemical compositions for two populations of m. pulegium, characterized by piperitone and pulegone. mentha spicata essential oil was characterized by endocyclic piperitenone epoxide, piperitone epoxide, and carvone. the bioactivities of these essential oils and their components have been tested against insect pests (leptinotarsa decemlineata, spodoptera littoralis and myzus persicae), root-knot nematodes (meloydogine javanica) and plants ...201727770481
natural variation in life history strategy of arabidopsis thaliana determines stress responses to drought and insects of different feeding guilds.plants are sessile organisms and, consequently, are exposed to a plethora of stresses in their local habitat. as a result, different populations of a species are subject to different selection pressures leading to adaptation to local conditions and intraspecific divergence. the annual brassicaceous plant arabidopsis thaliana is an attractive model for ecologists and evolutionary biologists due to the availability of a large collection of resequenced natural accessions. accessions of a. thaliana ...201728295823
impact of chemical, organic and bio-fertilizers application on bell pepper, capsicum annuum l. and biological parameters of myzus persicae (sulzer) (hem.: aphididae).myzus persicae (sulzer) is a polyphagous aphid that causes chlorosis, necrosis, stunting, and reduce growth rate of the host plants. in this research, the effects of zinc sulfate and vermicompost (30%), bacillus subtilis, pseudomonas fluorescens, glomus intraradices, g. intraradices × b. subtilis, and g. intraradices × p. fluorescens compared to control was investigated on the growth characters of capsicum annuum l. and biological parameters of m. persicae. different fertilizers caused a signifi ...201728283949
induction of systemic resistance against aphids by endophytic bacillus velezensis yc7010 via expressing phytoalexin deficient4 in arabidopsis.aphids are the most destructive insect pests. they suck the sap and transmit plant viruses, causing widespread yield loss of many crops. a multifunctional endophytic bacterial strain bacillus velezensis yc7010 has been found to induce systemic resistance against bacterial and fungal pathogens of rice. however, its activity against insects attack and underlying cellular and molecular defense mechanisms are not elucidated yet. here, we show that root drenching of arabidopsis seedlings with b. vele ...201728261260
the terpene synthase gene family in gossypium hirsutum harbors a linalool synthase ghtps12 implicated in direct defence responses against herbivores.herbivore-induced terpenes have been reported to function as ecological signals in plant-insect interactions. here, we showed that insect-induced cotton volatile blends contained 16 terpenoid compounds with a relatively high level of linalool. the high diversity of terpene production is derived from a large terpene synthase (tps) gene family. the tps gene family of gossypium hirsutum and gossypium raimondii consist of 46 and 41 members, respectively. twelve tps genes (ghtps4-15) could be isolate ...201829044662
phytophagy of omnivorous predator macrolophus pygmaeus affects performance of herbivores through induced plant defences.plants possess various inducible defences that result in synthesis of specialized metabolites in response to herbivory, which can interfere with the performance of herbivores of the same and other species. much less is known of the effects of plant feeding by omnivores. we found that previous feeding of the omnivorous predator macrolophus pygmaeus on sweet pepper plants significantly reduced reproduction of the two-spotted spider mite tetranychus urticae and western flower thrips frankliniella o ...201829124341
identification of the alarm pheromone of cowpea aphid, and comparison with two other aphididae species.in response to a predator attack, many aphidinae species release an alarm pheromone, which induces dispersal behavior in other individuals within the colony. the major component of this pheromone is the sesquiterpene (e)-β-farnesene (eβf), but variations occur between aphid species. in the present work, we collected, identified, and quantified the alarm pheromone of aphis craccivora koch (hemiptera: aphididae), before quantifying the escape behavior induced in the neighboring individuals. we com ...201829301046
attraction of aphidius ervi (hymenoptera: braconidae) and aphidoletes aphidimyza (diptera: cecidomyiidae) to sweet alyssum and assessment of plant resources effects on their fitness.the green peach aphid myzus persicae (sulzer) (hemiptera: aphididae) is one of the most economically important aphid species affecting crops worldwide. since many natural enemies of this aphid have been recorded, biological control of this pest might be a viable alternative to manage it. selected plant species in field margins might help to provide the natural enemies with food sources to enhance their fitness. this study aimed to investigate if sweet alyssum, lobularia maritima (l.) (brassicace ...201829365141
arabidopsis actin-depolymerizing factor3 is required for controlling aphid feeding from the phloem.the actin cytoskeleton network has an important role in plant cell growth, division, and stress response. actin-depolymerizing factors (adfs) are a group of actin-binding proteins that contribute to reorganization of the actin network. here, we show that the arabidopsis (arabidopsis thaliana) adf3 is required in the phloem for controlling infestation by myzus persicae sülzer, commonly known as the green peach aphid (gpa), which is an important phloem sap-consuming pest of more than fifty plant f ...201829133373
temporal and spatial distribution of insecticide-resistance mutations in the green peach aphid myzus persicae (hemiptera: aphididae) on primary and secondary host plants in central chile.the aphid myzus persicae develops insensitivity to almost all classes of insecticides through target site mutations. the aim of this study was to assess the temporal and spatial distribution of resistant aphids that grow on peach trees and weeds, and establish the role of the secondary hosts as a reservoir of resistant genotypes.201828834161
does multigenerational exposure to hormetic concentrations of imidacloprid precondition aphids for increased insecticide tolerance?hormetic preconditioning, whereby exposure to mild stress primes an organism to better tolerate subsequent stress, is well documented. it is unknown if exposure to hormetic concentrations of insecticide can trans-generationally prime insects to better tolerate insecticide exposure, or whether exposure to hormetic concentrations of insecticide can induce mutations in genes responsible for insecticide resistance. using the aphid myzus persicae (sulzer) and the insecticide imidacloprid as a model, ...201828905473
soil microbial species loss affects plant biomass and survival of an introduced bacterial strain, but not inducible plant defences.plant growth-promoting rhizobacteria (pgpr) strains can influence plant-insect interactions. however, little is known about the effect of changes in the soil bacterial community in general and especially the loss of rare soil microbes on these interactions. here, the influence of rare soil microbe reduction on induced systemic resistance (isr) in a wild ecotype of arabidopsis thaliana against the aphid myzus persicae was investigated.201829329376
non-plastidial expression of a synthetic insect geranyl pyrophosphate synthase effectively increases tobacco plant biomass.designing effective synthetic genes of interest is a fundamental step in plant synthetic biology for biomass. geranyl pyrophosphate (diphosphate) synthase (gpps) catalyzes a bottleneck step toward terpenoid metabolism. we previously designed and synthesized a plant (arabidopsis thaliana)-insect (myzus persicae, mp) gpps- human influenza hemagglutinin (ha) cdna, namely ptp-mpgpps-ha (or ptp-smpgpps-ha, s: synthetic), to localize the protein in plastids and improve plant biomass. to better underst ...201829277027
an ultraviolet b condition that affects growth and defense in arabidopsis.ultraviolet b light (uv-b, 280-315 nm) is the shortest wavelength of the solar spectrum reaching the surface of the earth. it has profound effects on plants, ranging from growth regulation to severe metabolic changes. low level uv-b mainly causes photomorphogenic effects while higher levels can induce stress, yet these effects tend to overlap. here we identified a condition that allows growth reduction without obvious detrimental stress in wild type arabidopsis rosette plants. this condition was ...201829362084
use of electrical penetration graphs (epg) and quantitative pcr to evaluate the relationship between feeding behaviour and pandora neoaphidis infection levels in green peach aphid, myzus persicae.a real-time qpcr method was developed, validated, and used to quantity the fungal pathogen, p. neoaphidis, within aphids at different times during infection; colonization rate fitted the gompertz model well (r2 = 0.9356). feeding behaviour of p. neoaphidis-infected and uninfected m. persicae were investigated, for the first time, using dc-electrical penetration graphs (dc-epg) that characterized the waveforms made during different aphid stylet probing periods corresponding to epidermis penetrati ...201829133229
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