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structural analysis of electrophoretic variation in the genome profiles of rotavirus field isolates.detailed structural studies were undertaken on five isolates of bovine rotavirus which showed variability in the migration patterns of their genome segments on electrophoresis in polyacrylamide gels. the individual genome segments of each isolate were characterized by partial digestion of terminally radiolabeled rna with a base-specific nuclease. this analysis showed that whereas mobility variations were always associated with detectable changes in nucleotide sequence, sequence changes at least ...19826282750
complement-fixation test for rotavirus detection: comparison and analysis of different methods to reduce anti-complementary activity of some specimens.the complement-fixation test may be used to detect rotaviral antigens directly in clinical specimens. however, a certain number of specimens tested for human rotaviruses by the complement-fixation test show an anti-complementary activity. by comparing eight techniques we analysed this anti-complementary activity and identified the best method for its reduction. pretreatment of clarified supernatant of stool suspensions by some methods resulted in a reduction of anti-complementary activity, witho ...19826282917
epidemiology of diarrhea due to rotavirus on an indian reservation: risk factors in the home environment.analysis of outpatient visits for diarrheal disease at the san carlos apache reservation, arizona, during 1977-1979 revealed a sharp increase in cases of infantile gastroenteritis of unknown etiology in october and november of each year. during the 1980 autumn diarrhea season, 19 patients and 12 control subjects were selected from among outpatients under two years of age and were interviewed and studied for bacterial and viral enteric pathogens. eleven (58%) of 19 patients and two (17%) of 12 co ...19826282985
rotavirus infections and the sompe syndrome. 19826282990
rapid multiple-determinant enzyme immunoassay for the detection of human rotavirus.enzyme immunoassays (eias) can be used to detect rotavirus antigens accurately in human stool specimens. conventional eia systems require prolonged incubations and multiple washing steps, thus making them impractical for truly rapid diagnosis. however, more rapid solid-phase eia systems can be devised which make use of simultaneous binding of enzyme-labeled and solid-phase antibodies at immunologically distinct antigenic sites. a multiple-determinant eia system was devised that was capable of de ...19826282992
the role of respiratory syncytial virus and other viral pathogens in acute otitis media.we utilized recently developed enzyme immunoassay techniques to examine the role of selected viruses in the etiology of acute otitis media. viral pathogens were found in middle ear fluids obtained from 13 (24%) of 53 children with acute otitis media; respiratory syncytial virus accounted for ten of the 13 viral agents identified. in addition, respiratory syncytial viral antigen was found in nasopharyngeal washings obtained from 15 of the 53 children. seven of these children had rsv identified as ...19826283052
new prospects for control of virus diarrhoea in children. 19826283077
gene protein products of sa11 simian rotavirus genome.when ma104 cells were infected with sa11 rotavirus, 12 protein classes, absent in mock-infected cells, could be distinguished by polyacrylamide gel electrophoresis. at least two of these proteins were glycosylated, and their synthesis could be blocked with tunicamycin. the oligosaccharides of both glycoproteins were cleaved by endo-beta-n-acetylglucosaminidase h, suggesting that they were residues of the "high-mannose" type. of the 12 viral polypeptides observed in infected cells, 1 was probably ...19826283128
molecular biology of rotaviruses. iii. isolation and characterization of temperature-sensitive mutants of bovine rotavirus.twenty-six temperature-sensitive (ts) mutants of united kingdom tissue culture-adapted bovine rotavirus were isolated and characterized. fourteen of these mutants were determined to be ts both by efficiency of plating and by virus yield at the nonpermissive temperature of 39.5 degrees c as compared with that at the permissive temperature of 32 degrees c. the remaining mutants were only ts by the criterion of efficiency of plating. high-frequency recombination (gene reassortment) was observed whe ...19826283176
age-dependent rotavirus-enterocyte interactions. 19826283556
incidence of rotavirus in artificially reared pigs and some effects of diarrhoea on the physiology and histology of the gastrointestinal tract.pigs reared on a milk substitute from two days old often developed diarrhoea, but suckled littermates remained healthy. only in a few pigs was diarrhoea associated with the presence of rotavirus. rotavirus was also present in some healthy pigs, and was associated with a reduction in villus length. pigs with diarrhoea usually had an increased amount of digesta in the stomach and a reduction in lactase activity in the small intestine but villus length was unchanged. there was no evidence of lactos ...19826283612
diarrhoea in dairy calves reduced by feeding colostrum from cows vaccinated with rotavirus.forty-two dairy calves remained with their dams for two days after birth, and then were removed to a calf rearing shed. calves were allocated to three groups for the next 14 days, and received twice daily either whole milk, whole milk with a 10 per cent supplement of pooled normal bovine colostrum or whole milk with 10 per cent supplement of colostrum from cows vaccinated with rotavirus. a natural outbreak of diarrhoea occurred, affecting 28 of the 42 calves. feeding immune colostrum delayed the ...19826283613
influence of humidity on rotavirus prevalence among nigerian infants and young children with gastroenteritis.rotaviruses were detected by counterimmunoelectro-osmophoresis in the feces of 16 (13.8%) of 116 infants and young children with gastroenteritis during a 5-month period (september 1979 through january 1980) in ife, nigeria. the rate of rotavirus detection varied inversely with relative humidity and was highest in december (38.5% positive) when the humidity was lower. there was not such a distinct relationship with temperature or vapor pressure; and although the rate of rotavirus detection was hi ...19826279690
porcine pararotavirus: detection, differentiation from rotavirus, and pathogenesis in gnotobiotic pigs.some characteristics of a newly recognized porcine enteric virus are described. tentatively, the virus was referred to as porcine pararotavirus (parv) because it resembled rotaviruses in respect to size, morphology, and tropism for villous enterocytes of the small intestine. however, it was antigenically distinct from porcine, human, and bovine rotaviruses and reoviruses 1, 2, and 3, and the electrophoretic migration pattern of parv double-stranded rna was distinct from the electrophoretic migra ...19826279693
acquisition of serum antibody to norwalk virus and rotavirus and relation to diarrhea in a longitudinal study of young children in rural bangladesh.serum antibodies to norwalk virus and to rotavirus were measured during longitudinal studies of infectious diseases and nutrition in rural bangladesh. initially, the prevalence of antibody to norwalk virus was 7% in children younger than six months and increased to 80% in children two to five years of age. the incidence of titer increases was highest in one- and two-year-olds and in children who had low or undetectable levels of antibody. some norwalk virus infections appeared to result in diarr ...19826279734
[rotavirus gastroenteritis (clinical course and diagnosis)]. 19826280328
pathology of experimental cv777 coronavirus enteritis in piglets. i. histological and histochemical study.sixteen cesarean-derived colostrum-deprived piglets were infected oronasally with cv777 coronavirus on the second or third day of life. two uninfected piglets were controls. they were killed at 96 and 120 hours after birth. after an incubation period of 22 to 36 hours, all principals showed severe diarrhea. the principals were killed between 12 and 120 hours after infection. exfoliation of enterocytes were seen first in the piglet killed 24 hours after infection (two hours after the diarrhea beg ...19826280359
[isolation of a cytopathogenic rotavirus from calves].a cytopathic rotavirus was isolated from calves with symptoms of diarrhea and underwent 15 passages in bovine embryo kidney cell culture. the specificity of the isolate was confirmed by the neutralization test, immunofluorescence, electron microscopy. the cpe of the isolated in bovine embryo kidney cells was similar to that of the reference strain.19826280392
rotavirus infection in nigerian infants and young children with gastroenteritis.in a study conducted over a 5-month period (september 1979 to january 1980), rotaviruses were detected by counterimmunoelectrophoresis in the feces of 16 (13.8%) of 116 infants and young children between 1 and 48 months old with acute, sporadic gastroenteritis, at ife, nigeria. the highest frequency of rotavirus infection was found in th 7- to 12-month age-group (18.9%). males constituted 61% of the sample and excreted rotavirus at a significantly higher rate than did females (p less than 0.01). ...19826280506
human rotavirus rna prepared from stool samples by a simple procedure suitable for the determination of electropherotypes. 19826281287
the differentiation of calf rotaviruses by plaque morphology and serum neutralization. 19826281378
multiple sclerosis. electrofocused "bands" of oligoclonal csf igg do not carry antibody activity against measles, varicella-zoster or rotaviruses.electrofocused serum and cerebrospinal fluid (csf) specimens from patients with multiple sclerosis (ms) were analysed for immunoglobulins (ig) and for antibodies to measles, varicella-zoster and rotaviruses by an imprint immunofixation method. patterns of intrathecally synthesized antibodies to the 3 viruses differed from patterns of oligoclonal igg in the csf. a variable proportion of virus antibody bands (average 19% for measles antibodies, 8% for varicella-zoster antibodies, 31% for rotavirus ...19826281394
rna of rotavirus: comparison of rnas of human and animal rotaviruses.single-stranded rna (ssrna) was transcribed in vitro from inner-shell particles of human rotavirus strain wa (hrv-wa) and a bovine rotavirus (neonatal calf diarrhea virus [ncdv]) by virion-associated rna polymerase activity. the ssrna product consisted of 11 rna segments which were separated by polyacrylamide gel electrophoresis. in vitro-transcribed 32p-labeled ssrna was used to study the genetic relatedness between rotaviruses by annealing with genomic double-stranded rna (dsrna) of homologous ...19826281481
viral gastroenteritis. 19826281591
radioimmunoassay in diagnostic virology. 19826281600
rotavirus infection in new zealand.rotavirus infection is commonly found in young infants admitted to hospital with gastroenteritis. an enzyme-linked immunosorbent assay (elisa) for virus diagnosis is described and the results of testing stool specimens from 497 children with gastroenteritis, 192 neonates and 247 asymptomatic six month old infants are presented. rotavirus infection was found in 45 percent of all children with gastroenteritis but only in 4.7 percent of neonates and 2 percent of asymptomatic infants. these results ...19826281699
rotavirus infection in otago: a serological study.a method for measuring rotavirus antibody in human sera has been established using enzyme-linked immunosorbent assay (elisa). a simian strain of rotavirus (sa11) was used as the antigen. serum eluted from dried blood spots on good quality chromatography paper was found suitable for analysis. paired serum samples from children with gastroenteritis have shown a brisk antibody response in association with the presence of rotavirus in the faeces. community studies indicate that although all older ch ...19826281701
rotavirus viral rna electrophoresis in hospitalized infants with diarrhea in santiago, chile.viral rna electrophoresis technique was used to detect rotavirus in 226 children under 2 years of age with acute diarrhea, admitted to the roberto del río hospital in santiago, chile, during the period of june 1979 through may 1980. a group of 50 children included in the aforementioned sample, admitted in winter, was compared with a control group of 25 infants without digestive pathology. in these groups, rotavirus was detected in 20 out of 50 children with diarrhea (40%) but not in the controls ...19826281717
[viral diarrhea].viruses are one of the most frequent causes of acute infectious gastroenteritis. thus, rotaviruses are responsible for 40-60%--and in winter sometimes for over 90% - of diarrhoea in infants. these viruses may also cause disease in adults, but small viruses of a size of about 27 nm, such as the norwalk agent, play a much more important role. several other non-cultivable viruses such as adeno-, calici- and astroviruses have been implicated as a cause of infectious diarrhoea. knowledge of rotavirus ...19826281877
inhibition of rotaviruses by selected antiviral substances: mechanisms of viral inhibition and in vivo activity.several rna virus inhibitors were evaluated against simian (sa11) rotavirus infections in vitro and murine rotavirus gastroenteritis in vivo. test compounds included 1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide (ribavirin), 3-deazaguanine (3-dg), 3-deazauridine, and 9-(s)-(2,3-dihydroxypropyl)adenine [(s)-dhpa]. all drugs inhibited total infectious sa11 virus yields in ma-104 cells. ribavirin, 3-dg, and (s)-dhpa affected [3h]uridine uptake into uninfected ma-104 cells in both the acid-sol ...19826282209
enteritis in foals induced by rotavirus and enterotoxigenic escherichia coli.colostrum-deprived, colostrum-fed or suckling foals were orally inoculated with foal rotavirus and enterotoxigenic escherichia coli derived from a calf. neither agent given alone caused diarrhoea in foals aged 1 or 2 days, although with rotavirus, 2 of the 3 inoculated foals became depressed 3 days after inoculation and all 3 were excreting rotavirus in the faeces. inoculation of both agents induced diarrhoea in colostrum-deprived, colostrum-fed or suckling foals aged up to 16 days. there was an ...19826282248
[production of monoclonal antibodies against bovine rotavirus]. 19826282403
[intestinal rotavirus infections (hrv - human rotavirus)]. 19826302630
[infectious diarrhea of viral and bacterial etiology]. 19826302816
[studies on the etiology of automnal acute gastroenteritis in kunming. i. a preliminary report on the detection of rotavirus]. 19826303613
[studies on the etiology of autumnal acute gastroenteritis in kunming. ii. determination of rotavirus by an enzyme-linked immunosorbent assay]. 19826303614
[detection of human rota virus by elisa using glass beads as solid phase]. 19826306921
[epidemic of acute gastroenteritis due to rotavirus in lianyungang city]. 19826309401
detection of rotavirus antibody by inhibition of reverse passive hemagglutination.a reverse passive hemagglutination inhibition (rphi) test was developed for detecting rotavirus antibody in patients' sera. sheep erythrocytes coated with guinea pig antibody against nebraska calf diarrhea virus (ncdv) were readily hemagglutinated by ncdv in a reaction called reverse passive hemagglutination (rpha). inhibition of this rpha reaction was used to detect the presence of rotavirus antibody in patients' sera which cross-reacted with ncdv. the sensitivity of rphi was consistently at le ...19826313739
assay of human rotavirus antibody by immune adherence hemagglutination with a cultivable human rotavirus as antigen.a cultivable human rotavirus, wa, was propagated in ma-104 cells and used as an antigen in immune adherence hemagglutination (iaha) and complement fixation (cf) tests. iaha antibody titers of sera from normal humans were found to be eightfold higher than cf antibody titers. the iaha antibody determination with the wa antigen is considered to be especially useful for the seroepidemiological studies of human rotavirus infections.19826313741
polymorphism of genomic rnas within rotavirus serotypes and subgroups.the genome of human rotaviruses is composed of 11 segments of double stranded rna. analysis by polyacrylamide gel electrophoresis of the genomic rna from a number of strains of the same serotype has demonstrated a significant polymorphism in the migration pattern. however, two strains differing from each other in serotype were found to have apparently identical rna migration patterns. it is concluded that serotype cannot be deduced from rna migration patterns. degrees of polymorphism have been o ...19826297431
sudden infant death syndrome associated with rotavirus infection.rotavirus was detected in the stools of five children stricken with sudden infant death syndrome (sids) over a three-week period. while none of the children had acute gastroenteritis, four of the five had acute upper respiratory infections. rotavirus was also identified in tracheal aspirates from two of the infants. extensive investigations failed to reveal the presence of any other viruses or toxins in specimens obtained from the five children with sids. rotavirus was not found in the stool spe ...19826298360
a sero-epizootiological survey on rotavirus infection in foals. 19826298495
propagation of bovine rotavirus by cats and dogs.spf cats were experimentally infected orally with bovine rotavirus. all of them excreted virus over a period of at least two weeks after inoculation. seroconversion was observed after one week for all the animals, but it did not stop viral excretion or prevent further excretion of the same or another rotavirus strain given later. cats or dogs kept in the same cage as inoculated animals became infected and excreted virus, but seroconversion was not observed in these contact animals. none of the a ...19826309066
[detection of rotavirus in healthy malagasian children]. 19826312908
identification of the rna segments encoding some structural polypeptides of nebraska calf diarrhea virus. 19826312525
[epidemiological study of rotavirus infections in premature infants]. 19826320707
infectious gastroenteritis in bone-marrow-transplant recipients.we prospectively evaluated infections with several gastrointestinal pathogens in patients undergoing bone-marrow transplantation, in an attempt to correlate infection with morbidity and mortality. thirty-one of 78 patients (40 per cent) were infected with one or more of the following enteric pathogens during the study: adenovirus (12 infections), rotavirus (nine), coxsackievirus (four), or clostridium difficile (12). several patients were infected with more than one pathogen. infection correlate ...19827038501
studies with an unclassified virus isolated from diarrheic calves.a transmissible agent (breda agent) was isolated from a calf with diarrhea and shown to be infectious by inoculation orally into gnotobiotic and conventionally reared calves. the "breda" agent had the morphology of a virus and possessed a hemagglutinin. antigenic studies showed the virus to be antigenically different from bovine coronavirus, parainfluenza 3 virus, bovine rotavirus, bovine parvovirus and bovine pestivirus (bvd). attempts to culture the virus in cell or organ cultures or in embryo ...19827051518
[cryptosporidium: a "new" factor in the aetiology of neonatal diarrhoea in calves].in recent years, cryptosporidium, a coccidian parasite of the intestine, has been associated with neonatal diarrhoea in calves. current knowledge of the organism with regard to classification, host-specificity, life cycle, pathology, incidence, clinical treatment, prevention and diagnosis is reviewed in the present paper. in addition, cryptosporidium infection in calves in the netherlands is reported for the first time. the typical 4 micrometer oocysts were observed on microscopic examination of ...19827123530
rotavirus gastroenteritis and weather.during 5.5 years of a study in washington, d.c., hospitalizations of children for rotavirus gastroenteritis tended to be more common after a month of cold or dry weather than after a corresponding calendar month of warm or wet weather. overall, there were 84% more (178 versus 97) inpatients with rotavirus gastroenteritis after a set of relatively colder individual months taken as a group than after an equal number of warmer corresponding calendar months taken as a group. comparable differences w ...19827130360
intestinal changes in gnotobiotic piglets experimentally inoculated with porcine rotavirus.intestinal lesions were studied in 8 gnotobiotic piglets orally given porcine rotavirus by light microscopy, scanning electron microscopy and transmission electron microscopy. five of these piglets were killed 6, 12, 18, 24 and 48 hours after inoculation, respectively. of the other 3 piglets, one died 60 hours and two became moribund 96 and 114 hours after inoculation, respectively. all the infected piglets showed diarrhea between 18 and 24 hours after inoculation. examination of the small intes ...19827133149
[the aerobic bacterial intestinal flora of various wintering geese species].the aerobic fecal flora of wintering brent goos (branta bernicla), barnacle goose (branta leucopsis), greylag goose (anser anser), white-fronted goose (anser albifrons), pink-footed goose (anser brachyrhynchus), and bean goose (anser fabalis) was studied. there were no specific differences between the various geese. bacterial counts were in the range of 10(5)-10(7) cpu per gram of feces. neither pathogenic bacteria nor rotavirus could be detected in the fecal samples of the wintering geese, so t ...19827136353
[rotavirus infections in a pediatric section of an infectious disease hospital department supervised in march-may 1981]. 19827144711
diarrhea in breast-fed and non-breast-fed infants.during the first year of life a group of babies was prospectively observed for diarrhea and for fecal carriage of heat-labile toxigenic bacteria, with or without colonization factor, and rotavirus. approximately half of the babies were breast-fed for the first six months of life. there was no difference between groups (breast-fed vs non-breast-fed) in number of babies who had diarrhea during any two-month period. nor was there any difference between groups in the number of babies who had diarrhe ...19827145548
diarrhoea in foals associated with rotavirus. 19827147670
carbohydrate malabsorption in infants with rotavirus diarrhea.we studied a group of patients with rotavirus diarrhea to determine the association of carbohydrate malabsorption during diarrhea with the degree of acidosis and severity of purging. unlike enterotoxigenic diarrhea in which the metabolic acidosis is due to loss of bicarbonate in an alkaline stool, patients with rotavirus develop a metabolic acidosis while passing an acid stool with little detectable bicarbonate. also unlike enterotoxigenic diarrhea, rotavirus stool contains large quantities of r ...19827148733
epidemiology of rotavirus diarrhea in yogyakarta, indonesia, as revealed by electrophoresis of genome rna.nine different electropherotypes of rotaviruses occurred among 85 children with rotavirus diarrhea in yogyakarta, indonesia. eighty percent of the electropherotypes had a "long" rna pattern characteristic of human subgroup 2.19827153319
study of rotavirus diarrhea in a north indian community. 19827160902
diarrhoeal problems in southeast asia.diarrhoea up till now is still a major problem in southeast asia with high morbidity and mortality, particularly among children under 5 years of age, with the peak in children between 6 - 24 months. in indonesia, in 1981, it was estimated that there are 60 million episodes with 300,000 - 500,000 deaths. in the philippines, diarrhoea ranks as a second cause of morbidity (600 per 100,000 in 1974) and second cause of infant mortality (5 per 1,000 in 1974). in thailand, in 1980, the morbidity rate w ...19827163834
a long term study of rotavirus infection in thai infants and children with diarrhoea.during 1977-1980, human rotavirus was detected by electron microscopic technique in the stool of 55 (30.7%) of 179 patients, hospitalized in bangkok metropolis. characteristic particles 65-70 nm in diameter, round shape, resembling a little wheel with radius capsomeres from the dense core. other some adenovirus and small virus particles were seen, 2.8% and 2.2% respectively. seasonal distribution among thai patients with rotavirus infection mostly occurred in january (58.8%). the youngest patien ...19827163842
morbidity and mortality study on diarrhoeal diseases in north jakarta - an urban area.diarrhoea including its interaction with undernutrition is one of the most important health problems and a major cause of death in young children in developing countries including indonesia. to quantify the problems and to have comparison with results of other diarrhoeal surveys in indonesia as well as in other developing countries, a survey was done in urban community comprising a population of 5,115 living in a relatively good area which was not a slum, nor wealthy area. the incidence of diarr ...19827163847
[role of viruses in acute gastroenteritis].in a two years screening carried out on florentine children we confirmed that rotaviruses are the most important ethiological agents of acute gastroenteritis in infants less than two years old, irrespective of sex. we had evidence that rotavirus diarrhea does not occur with the same incidence every year. rotavirus gastroenteritis is characterized by profuse diarrhea, vomiting, fever and often by respiratory symptoms. severe complications have not been observed.19827170197
diarrhea in day care centers. 19827177896
[useful and superfluous measures in the treatment of infant diarrhea].in acute diarrhea of infancy we distinguish between infectious and noninfectious causes. in the latter we know some autosomal recessive disorders, e.g. the glucose-galactose-malabsorption, the lactase deficiency as well as the sucrase-isomaltase deficiency. in addition the most frequent acquired disorders like the cow's milk protein intolerance and celiac disease contribute also to the group of noninfectious causes of diarrhea. here the most effective therapy consists of the elimination of the t ...19827178037
etiology of hospital-acquired infectious diarrhea. 19827181595
enterotoxigenic escherichia coli and other enteropathogens in paediatric diarrhoea in addis ababa.this study was performed during two weeks among 86 paediatric outpatients of poor socio-economic background. a control group comprised 60 healthy children. enterotoxigenic escherichia coli (etec) was the most common diarrhoeal agent isolated (26%). strains of etec producing heat-labile (lt) only or lt and heat-stable (st) enterotoxin were isolated from 11% each and etec producing st only from 4% of the patients. etec was also found not infrequently among controls (10%). etec with o-antigens 78, ...19826753473
colonization of the upper jejunum by enteropathogenic and enterotoxigenic escherichia coli in paediatric diarrhoea.aspirated fluid from the upper jejunum was obtained by intubation of 27 children with diarrhoea and 7 control children without diarrhoea. the aspirated jejunal fluid was analysed for total counts of viable aerobic and anaerobic microorganisms. gram-negative aerobic bacteria were typed biochemically and analysed for the production of heat-labile and heat-stable enterotoxin. enteropathogenic escherichia coli (epec) strains of serogroups 0111, 055 and 0127 were detected in the stools of nine patien ...19826753475
stool electrolyte composition in relation to etiology in acute gastroenteritis. 19826754604
theory and practice of immunoprophylaxis in swine. 19826757217
diarrhoea in swedish infants. aetiology and clinical appearance.ninety-five children with gastroenteritis admitted at the department of paediatrics, malmö general hospital, malmö, sweden, starting november 1978 through october 1979 were studied. patients were examined clinically and the course of their disease was followed. stool specimens from all patients were analysed by routine bacteriology and rotavirus identification was done by immune-electroos-mophoresis. fifty-eight per cent of patients had rotavirus. campylobacter was found in 6%, enteropathogenic ...19826760665
rotavirus infections in a special-care baby unit. 19826764499
mechanisms and mediators of intestinal secretion in the small intestine. 19826764511
enzyme immunoassays for the detection of infectious antigens in body fluids: current limitations and future prospects.enzyme immunoassays are attaining increased usage for the direct detection of microbial antigens in body fluids. advantages of enzyme immunoassays include a high degree of sensitivity resulting from the inherent magnification of the enzyme-substrate reaction and the use of objective end points without the need for radioactivity. enzyme immunoassays have been developed for the reliable detection of several important microbial antigens in body fluids, including antigens of rotavirus, hepatitis b v ...19826803327
surveillance of patients attending a diarrhoeal disease hospital in bangladesh.in october 1979 a surveillance system was set up at the international centre for diarrhoeal disease research, bangladesh, hospital at dacca to study a 4% systematic sample of the 100 000 patients with diarrhoea who come to the hospital for care each year. from december 1979 to november 1980 inclusive, 3550 patients were studied. a recognised pathogenic organism was identified for 66% of patients screened for all pathogens, one-third of whom had a mixed infection with two or more agents. enteroto ...19826812801
[fecal detection of rotavirus and other enteropathogens in infants less than a year old, with acute diarrhea]. 19826817391
clinical features of rotavirus gastroenteritis. 19827185988
[sero-epidemiologic study of the occurrence of rotavirus infection in the roman population]. 19827187035
[rotavirus seroepidemiology: predominance of precipitating antibodies in serum of children between the ages 0 to 12]. 19827187042
serological survey on rotaviral antibody in the hungarian swine population by optimized counter-current immuno-electrophoresis (cciep). 19827187805
[serological signs of nosocomial infections by rotavirus in a pediatric ward for infectious diseases]. 19827188245
morphology of intestinal changes in pigs experimentally infected with porcine rota-virus and two porcine corona viruses. 19826959242
rotavirus associated diarrhoea in nursing piglets and detection of antibody against rotavirus in colostrum, milk and serum.a blocking method of elisa for the detection and quantification of antibody against porcine rotavirus in serum, colostrum and milk has been compared with a plaque reduction test. the results obtained with the two techniques correlated (fig. 1). antibody against rotavirus was demonstrated in 384 serum samples representing 25 swine farms, indicating a widely spread and dense distribution of the infection with porcine rotavirus among danish swine. the antibody contents in milk samples from 7 gilts ...19836326047
necrotizing enterocolitis and human rotavirus. 19836571531
gastroenteritis in children. 19836571966
[immunofluorescence reaction for rotavirus analysis in fecal specimens].testing for rotavirus detection in faeces from infants with acute diarrhea can be carried out, with high sensitivity, by immunofluorescence on rhesus monkey kidney cell lines (ma-104). preincubation of viral inoculum with trypsin enhances further the sensitivity. in the present study we tried to assess the optimal conditions for detection of viral antigens, by analyzing the expression of virus-specific proteins at different times of infection and the effects of trypsin presence during virus adso ...19836330786
an outbreak of rotavirus gastroenteritis on a pediatric unit. 19836554053
influence of temperature and relative humidity on human rotavirus infection in japan.a climatologic analysis of human rotavirus infection in inpatients with acute diarrhea was conducted over a seven-year period. the infection frequency appeared to be related to temperature, but not to relative humidity. human rotavirus infection was found to appear abruptly when the mean temperature of any 10-day period became less than 5 c (november or december), reached a peak when it was less than 0 c (january and february), and waned when it became greater than 20 c (june and july) in the ci ...19836822748
differential diagnosis of scouring in lambs. 19836832843
microtiter solid-phase radioimmunoassay for detection of human calicivirus in stools.a microtiter solid-phase radioimmunoassay (ria) was developed for detection of human calicivirus in stool specimens. seventy-eight stool specimens were tested by ria. all 17 specimens positive for human calicivirus by electron microscopy (em) were also positive by ria. in addition, of 21 specimens obtained from an outbreak of caliciviral gastroenteritis, 11 were positive by ria but negative by em. of 20 specimens positive for rotavirus by em and 20 nondiarrheic specimens with no virus, 2 and 1, ...19836833476
rotavirus infections in a pediatric clinic. 19836840056
[rotavirus infection in children--clinical aspects]. 19836847327
[rotavirus infections--epidemiological aspects]. 19836847329
influence of malnutrition and alterations in dietary protein on murine rotaviral disease.the possible correlation between malnutrition and degree of severity of rotavirus-associated infantile diarrhea which appears to occur in human populations was studied using a mouse model. to determine the effects of general malnutrition or altered levels of dietary protein, female mice were fed throughout pregnancy and infection periods with diets diluted with 0, 300, or 600 g glucose/kg, designated as normal nutrient to calorie ratio (n/c) diet, 70% n/c diet, or 40% n/c diet or with diets cont ...19836867015
[frequency of acute infectious gastroenteritis caused by rotavirus in children of various populations of the mexican republic]. 19836871004
the microbiology of childhood gastroenteritis: aeromonas species and other infective agents.a prospective, 12-month study of 975 non-aboriginal children with diarrhea and age- and sex-matched children without diarrhea, in perth, western australia, was designed to investigate the significance of enterotoxigenic aeromonas species as a cause of diarrhea. enterotoxigenic aeromonas species were found in the fecal specimens of 10.8% of the patients with diarrhea but in only 0.7% of those without diarrhea. most aeromonas species were isolated during the summer. other important bacterial patho ...19836886488
investigation of acute gastroenteritis in general practice--relevance of newer laboratory methods.over a nine-month period, all patients suffering from acute gastroenteritis, with diarrhoea as an essential component, who presented to a group practice in southern england were investigated using conventional laboratory methods, and also newer techniques of electron microscopy and search for species of campylobacter. rotavirus and campylobacter were the two most commonly encountered pathogens.19836887127
a significant role of rotavirus in acute gastroenteritis in infants and young children. 19836888941
plaque assay of bovine rotavirus. 19836326542
viral gastroenteritis and laboratory detection of rotavirus.two major viruses have been clearly associated with gastroenteritis: the norwalk agent and the rotavirus. the former is associated with epidemic outbreaks in adults or schoolchildren, while the latter causes endemic illness in young children during winter. neither can be grown in routine cell cultures. both of these viruses, and others, can be detected in patients' stools by electron microscopy. enzyme linked immunosorbent assay (elisa) kits are commercially available for detection of rotavirus ...19836326572
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