Publications

immunochemistry of thioredoxin. i. preparation and cross-reactivity of antibodies against thioredoxin from escherichia coli and bacteriophage t4.		1972	4113817
bacteriophage t4 head morphogenesis. 3. some novel properties of gene 13-defective heads.	the nature of phage precursors in gene 13-defective infected cells was studied by electron microscopy and pulse-chase isotopic labeling experiments. our results suggest that both stable (20%) and fragile (70%) filled-head precursors accumulated in the absence of gene 13 product. upon extraction, the fragile heads were found to lose most of their deoxyribonucleic acid and appeared unfilled with an average density of 1.34 g/cm(3) and a sedimentation coefficient of 300s. these unfilled heads differ ...	1972	4113885
nuclear disruption after infection of escherichia coli with a bacteriophage t4 mutant unable to induce endonuclease ii.	nuclear disruption after infection of escherichia coli with a bacteriophage t4 mutant deficient in the ability to induce endonuclease ii indicates that either (i) the endonuclease ii-catalyzed reaction is not the first step in host deoxyribonucleic acid (dna) breakdown or (ii) nuclear disruption is independent of nucleolytic cleavage of the host chromosome. m-band analysis demonstrates that the host dna remains membrane-bound after infection with either an endonuclease ii-deficient mutant or t4 ...	1972	4114321
serum pattern of thyroxine (t4) and triiodothyronine (t3) after treatment of thyrotoxicosis with antithyroid drugs.		1972	4114923
[evaluation of the simple determination of serum t4-i by west's method].		1972	4115660
mouse thymus-independent and thymus-derived lymphoid cells. i. immunofluorescent and functional studies.	the simultaneous use on mouse lymphoid suspensions of heterologous antisera directed against thymus-derived (t) cell mouse-specific lymphocyte antigen and brain-associated theta antigen (msla and batheta) or thymus-independent (b) cell mouse-specific bone marrow-derived lymphocyte antigen (mbla) surface antigens allowed direct proof of the different specificity of these antisera by double immunofluorescence (if) staining with selective visualization of fluorochromes. these antisera and antisera ...	1972	4117194
efficiency of protein and messenger rna synthesis in bacteriophage t4-infected cells of escherichia coli.		1972	4567464
bacteriophage t4 transfer rna. 3. clustering of the genes for the t4 transfer rna's.		1972	4567465
metabolism of t4 messenger rna, host messenger rna and ribosomal rna in t4-infected escherichia coli b.		1972	4567471
lack of discrimination between early and late t4 mrna by host initiation factors.		1972	4573695
size of polysome involved in the synthesis of t4 phage lysozyme.		1972	4575192
effect of nalidixic acid on the growth of deoxyribonucleic acid bacteriophages.	the effect of nalidixic acid on the growth of various deoxyribonucleic acid (dna) bacteriophages has been investigated by one-step growth experiments. the escherichia coli bacteriophages t5, lambda, t7 and phir are strongly inhibited by nalidixic acid, whereas t4 and t2 are only partially inhibited. the bacillus subtilis bacteriophages sp82, sp50, and phi29 are relatively unaffected by nalidixic acid. there is no correlation between those bacteriophages which can grow in the presence of nalidixi ...	1972	4621668
metabolism of t4 bacteriophage ghost-infected cells. ii. do ghosts cause a generalized permeability change?	ghosts appear to alter the barrier function of the cell membrane, allowing the release of phosphorylated sugars which normally cannot pass through the cell membrane, whereas phage do not. no increased influx of normally impermeable compounds is observed in the presence of ghosts, indicating that the loss of membrane function after ghost infection cannot be attributed to a generalized breakdown of the permeability barrier.	1972	4624785
product of t4 gene 12.		1972	4624817
[induction of mutations in the bacteriophage t4 by a treatment of bacteria with 2 amino-purine before the infection].		1972	4625040
human prolactin and thyrotropin concentrations in the serums of normal and hypopituitary children before and after the administration of synthetic thyrotropin-releasing hormone.	synthetic thyrotropin-releasing hormone (trh) was administered to normal children and hypopituitary patients in a dose of 7 mug/kg i.v. over 30-60 sec. serum thyrotropin (tsh) and prolactin (hpr) concentrations were measured by radioimmunoassay before and at 15-min intervals for 2 hr after trh. in 20 normal children hpr rose from a mean baseline value of 7.0+/-1.2 (sem) ng/ml to a mean peak value of 39.5+/-5 ng/ml. in 11 patients with growth hormone (gh) deficiency without tsh deficiency. hpr va ...	1972	4626583
use of a clonal line of porcine kidney cell cultures for primary isolation and vaccine studies with adenoviruses.	the clonal line (y15) of porcine kidney stable cells provided a recovery system for adenovirus t4 from specimens from adults with respiratory illnesses that was as sensitive as human embryo kidney cultures. adenoviruses t7 from adults, and t1, 2, 3, and 5 from children could be readily isolated in porcine kidney cell cultures. the latter were useful for adenovirus vaccine studies in that infectivity titers of live virus vaccine and neutralization antibody responses after vaccination were equal t ...	1972	4629702
[various results of t3 and t4 tests in autonomous thyroid nodules before and after treatment].		1972	4639003
chemical synthesis of two deoxyribopolynucleotide fragments containing the natural sequence of t4 lysozyme gene e.		1972	4640369
[reciprocal action of dna dependent rna polymerase with t4-dna and desoxyribonucleotides].		1972	4649768
[comparison of automated chemical determination of plasma hormonal iodine and thyroxinemia measured by competition (t4 test)].		1972	4661591
a genetic assay for transversion mutations in bacteriophage t4.		1972	4662742
[new semi-automated method for the determination of serum thyroxine(t4) using the t4 column (oxford company) and autoanalyzer--a new method using chloride as an activator].		1972	4674583
synergistic inactivation of viruses by heat and ionizing radiation.	viral inactivation by heat and/or ionizing radiation is analyzed in terms of a kinetic model. the phenomenon of synergistic viral inactivation observed when viruses are exposed to the simultaneous application of heat and ionizing radiation is interpreted within the framework provided by this three-term model. data on the inactivation of t4 bacteriophage by heat and/or ionizing radiation is presented, and the kinetic model is used to provide a description of observed dose rate and temperature dep ...	1972	5007243
structure of the tubular variants of the head of bacteriophage t4 (polyheads). i. arrangement of subunits in some classes of polyheads.		1972	5023668
structure of the tubular variants of the head of bacteriophage t4 (polyheads). ii. structural transition from a hexamer to a 6+1 morphological unit.		1972	5023669
coordinate variation in lengths of deoxyribonucleic acid molecules and head lengths in morphological variants of bacteriophage t4.	we have investigated three classes of small bacteriophage t4 particles which differ from normal t4 particles in length of their deoxyribonucleic acid (dna), in head length, in protein content, and in density. the different particles contain dna molecules measuring 0.90, 0.77, or 0.67, respectively, of the normal t4 length. an additional class of viable particles contains dna molecules of 1.1 unit length. these discrete differences in dna length correspond to discrete differences in length (but n ...	1972	5025493
bacteriophage t4 head models based on icosahedral symmetry.	in an accompanying paper, the coordinate variation in lengths of deoxyribonucleic acid molecules and head lengths in morphological variants of bacteriophage t4 is discussed. as an appendix to that report, models for the t4 phage heads, based on previously developed architectural principles for assembly of icosahedral virus, are compared with the data for the best fit.	1972	5025494
on the repair mechanism responsible for multiplicity reactivation in bacteriophage t4.		1972	5025510
ribonucleotide reductase genes of phage t4: map location of the thioredoxin gene nrdc.		1972	5039026
characterization by electron microscopy of the complex formed between t4 bacteriophage gene 32-protein and dna.		1972	5045301
the structure of rii diploids of phage t4.		1972	5047921
the isolation and characterization of a t4 mutant partially defective in recombination.		1972	5047922
spectral changes associated with binding of folate compounds to bacteriophage t4 dihydrofolate reductase.		1972	5057079
[triiodothyronine (t3) and thyroxine (t4) ratio in the thyroid tissue of normal japanese, with special reference to consumption of seaweeds].		1972	5063673
uptake of bacteriophage and their subsequent survival in edible west coast crabs after processing.	edible west coast crabs (cancer magister and cancer antennarius), when in seawater contaminated with coliphage t4, were found to accumulate high titers of this virus. to study the extent of the hazard presented by crabs contaminated with virus, samples of edible crabs were contaminated with coliphage t4 and then processed by boiling. results indicated that virus in crabs withstood this method of processing. the survival rate varied from 2.5 to 20%, depending upon the processing time. heat penetr ...	1972	5064986
metabolism of l-thyroxine (t4) and l-triiodothyronine (t3) by human fibroblasts in tissue culture: evidence for cellular binding proteins and conversion of t4 to t3.		1972	5065814
[isolation of specific polymerase binding sites on t4-dna].		1972	5069315
bacteriophage t4 transfer rna. ii. mutants of t4 defective in the formation of functional suppressor transfer rna.		1972	5069794
morphologenesis of the tail fiber of bacteriophage t4. iv. isolation and characterization of a component (the genes 36-37-38 directing component).		1972	5069823
synthesis of atp- and datp-substituted sepharoses and their application in the purification of phage-t4-induced ribonucleotide reductase.		1972	5081610
[t3 and t4 content of thyroid secretion according to iodine supply. a model based on the available data].		1972	5083356
genetic and immunological studies of bacteriophage t4 thymidylate synthetase.	thymidylate synthetase, which appears after infection of escherichia coli with bacteriophage t4, has been partially purified. the phage enzyme is immunologically distinct from the host enzyme and has a molecular weight of 50,000 in comparison to 68,000 for the host enzyme. a system has been developed to characterize t4 td mutants previously known to have impaired expression of phage thymidylate synthetase. for this system, an e. coli host lacking thymidylate synthetase was isolated. known geneti ...	1973	4575286
cadaverine in bacteriophage t4.	cadaverine was found in bacteriophage t4 when the host cells of escherichia coli k-12 were grown in complex media and aerated by agitation. only traces of cadaverine were found if the host was grown and agitated in synthetic medium or was aerated by vigorous bubbling in a complex medium. when the host cells were grown anaerobically in a complex medium, cadaverine became the major polyamine in the progeny phage. the polyamine content comprised 80% cadaverine, 14% spermidine (or its recently disco ...	1973	4575287
analysis of nuclear disruption and binding of intermediates in host dna breakdown to membranes after infection of escherichia coli with bacteriophages t4 and t7.	escherichia coli dna polymerase i is implicated in the binding of intermediates in host dna breakdown to membrane in t4-infected, but not t7-infected, cells. nuclear disruption is observed in t4-infected pola1 mutant cells.	1973	4575288
nondiscrimination of rna viral message in binding to 30s ribosomes derived from t4 phage infected escherichia coli.		1973	4575786
inhibition of t4 maturation by tri-l-ornithine.		1973	4575982
association of the riia protein with the bacterial membrane.	cell membrane proteins synthesized after infection of escherichia coli b with wild-type phage t4 and riia mutants were analyzed by dodecyl sulfate-polyacrylamide gel electrophoresis. a protein with an approximate molecular weight of 74,000 is present in membranes isolated from t4r(+)-infected cells, but is not found in membranes prepared from cells infected with an riia mutant in which the major part of the riia cistron is deleted. in addition, infection of e. coli b with different riia amber mu ...	1973	4576020
utilization of aminopurine deoxynucleoside triphosphate by mutator, antimutator and wild-type dna polymerases of bacteriophage t4.		1973	4576118
a negative effect of -glucosylation on t4 growth in certain rna polymerase mutants of escherichia coli: genetic evidence implicating pyrimidine-rich sequences of dna in transcription.		1973	4576342
isolation and characterization of mutants of bacteriophage t4 resistant to folate analogs.		1973	4576345
the immunity reaction of bacteriophage t4: a noncatalytic reaction.		1973	4576346
role of gene 52 in bacteriophage t4 dna synthesis.	in an attempt to elucidate the mechanism of delayed dna synthesis in phage t4, escherichia coli b cells were infected with h17 (an amber mutant defective in gene 52 possessing a "dna-delay" phenotype). the fate of (14)c-labeled h17 parental dna after infection was followed: we could show that this dna sediments more slowly in neutral sucrose than wild-type dna 3 min postinfection. in pulse-chase experiments progeny dna was found to undergo detachment from the membrane at 12 min postinfection. re ...	1973	4576517
the influence of glucosylation on the renaturation rate of t4 phage dna.		1973	4577829
abortive bacteriophage t4 head assembly in mutants of escherichia coli.		1973	4578101
change in rna polymerase associated with the shutoff of host transcription by t4.		1973	4578171
a dna-binding protein induced by bacteriophage t7.	a dna-binding protein has been purified from escherichia coli infected with bacteriophage t7 by dna-cellulose chromatography. the protein is absent in uninfected cells. the purified protein has a molecular weight of 31,000 and binds strongly and preferentially to single-stranded dna. in vitro studies show that this protein can stimulate the rate of polymerization catalyzed by the t7-induced dna polymerase 10-15 times under conditions where the polymerase is unable to effectively use a single-str ...	1973	4578445
improved preparation of bacteriophage t4-infected escherichia coli for the isolation of polynucleotide kinase.		1973	4578550
polypeptide bound to the host rna polymerase is specified by t4 control gene 33.		1973	4579464
an immunochemical characterization of glucosylation in bacteriophage t4.		1973	4579548
multiple and specific initiation of t4 dna replication.	partially replicated t4 dna molecules (prm) whose parental or progeny dna was labeled with bromodeoxyuridine budr was analyzed by gradual shearing followed by cscl banding of the sheared product. analysis of prm containing 18-mum replicated dna showed that each replicated region was 3- to 6-mum long, indicating three to 6 replicative sites per molecule. analysis of prm containing 9-mum replicated dna similarly indicated two to three replicated regions per molecule. dna from the replicated region ...	1973	4579821
mutagenic effect of temperature-sensitive mutants of gene 42 (dcmp hydroxymethylase) of bacteriophage t4.	certain temperature-sensitive mutants of gene 42 of bacteriophage t4 increase the reversion rates of some rii mutants in the same genome by about 4 to 10 times. this effect was usually found at 34 c, an intermediate permissive temperature, but not at 28 c.	1973	4579822
injection of ultraviolet-damage-specific enzyme by t4 bacteriophage.	when uv-irradiated t4 bacteriophage (v(+)) infects in the presence of chloramphenicol, the phage dna rapidly acquires single-stranded breaks proportional to the dose of uv. in contrast, when uv-irradiated t4 v(1) (radiation sensitive mutant) infects under identical conditions, the phage dna remains integral. a series of coinfections with v(+) and v(1) phage (uv-v(1) + majority non-uv-v(+) and uv-v(+) and majority non-uv-v(1)) show that the enzyme responsible for breakage is injected by the phage ...	1973	4579823
regulation of early mrna synthesis in bacteriophage t4-infected bacteria: dependence on bacteriophage-specific protein synthesis.	the mechanism of the transcriptional turn-off was studied for those species of mrna in t4 infection which specify early phage proteins. total synthesis of these early rna species was determined by short-time labeling with uracil-5-(3)h after the infection of escherichia coli b with dna-negative, conditional lethal mutants, which produce no late mrna under nonpermissive conditions. with both amber and temperature-sensitive mutants a decrease in incorporation into early rna by more than 90% was ob ...	1973	4579824
control of t4 endonuclease iv by the d2a region of bacteriophage t4.		1973	4579886
circular and branched circular concatenates as possible intermediates in bacteriophage t4 dna replication.		1973	4580243
electron microscopy of polyheads of bacteriophage t4 prepared by freeze-etching.		1973	4580470
bacteriophage t4 tail assembly: structural proteins and their genetic identification.		1973	4580679
bacteriophage t4 tail assembly: proteins of the sheath, core and baseplate.		1973	4580680
hk239: a p2 related temperate phage which excludes rii mutants of t4.		1973	4580728
the chemical synthesis of deoxyribo-oligonucleotides complementary to a portion of the lysozyme gene of phage t4 and their hybridization to phage specific rna and phage dna.		1973	4581002
[proteins of bacteriophage t4 head (author's transl)].		1973	4582331
reactions at the 3' terminus of transfer ribonucleic acid. ix. trna nucleotidyltransferase activity in bacteriophage t4-infected escherichia coli.		1973	4582376
functions of baseplate components in bacteriophage t4 infection. ii. products of genes 5, 6, 7, 8, and 10.		1973	4582603
an extended phage growth cycle in t4 infected a. aerogenes.		1973	4582604
purification of thioredoxin from escherichia coli and bacteriophage t4 by immunoadsorbent affinity chromatography.		1973	4582821
functions of rii genes of t4 bacteriophage. communication i. structure of replicating dna of bacteriophage t4r+ and t4r 1272 in e. coli cells.		1973	4582984
ribosomal assembly influenced by growth in the presence of streptomycin.	translational leakiness (i.e., nonspecific suppression) of nonsense mutants of bacteriophage t4 is increased in cells of certain streptomycin-resistant strains previously grown in the presence of streptomycin. concomitantly, ribosomes extracted from these streptomycin-grown cells possess a high level of misreading. increased suppression ability as well as ribosomes that highly misread accumulate with kinetics expected for a constant differential rate of synthesis of a new product induced by drug ...	1973	4583024
a specific inhibitor of polypeptide-chain initiation in escherichia coli.	an inhibitor of polypeptide-chain initiation was isolated from e. coli cells. this protein inhibits formation of the 30s or 70s initiation complex with either fmet-trna(f) as initiator and aug, ms2 rna, or late t4 rna as messenger, or acphe-trna as initiator and poly(u) as messenger. chain elongation, e.g., poly(u) translation at high mg(2+) concentration, is not inhibited. the inhibitor is rendered ineffective when active aminoacylation of trna is taking place, e.g., during natural mrna transla ...	1973	4583026
hydroxyurea-sensitive mutants of t4. ii. degradation and utilization of bacterial dna.		1973	4583303
control of bacteriophage t4 dna polymerase synthesis.		1973	4583371
control of gene function in bacteriophage t4. iv. post-transcriptional shutoff of expression of early genes.	the selective and sequential shutoff of synthesis of early t4 proteins in bacteria infected with dna-negative mutants is under the active control of one or more t4-induced proteins. selective shutoff of synthesis of early t4 proteins is accompanied by a selective degradation of distinct species of t4 mrna. we present circumstantial evidence that selective degradation of mrna is the cause, and not the consequence, of selective termination of expression of early t4 genes. the mutation sp62 inactiv ...	1973	4583805
host-mediated repair of discontinuities in dna from t4 bacteriophage.	discontinuities of t4 dna which are caused by excision of uv-damaged areas, by decay of (32)p atoms, or which are present in dna from rii(-)lig(am) (-) phage produced in a host nonpermissive for amber mutants are all repaired by bacterial enzymes after infection in the presence of chloramphenicol. escherichia coli dna polymerase i participates in the host-mediated repair, but an approximately 20-fold variation in the levels of host polynucleotide ligase does not affect either the kinetics or the ...	1973	4583887
effect of a gene-specific suppressor mutation (das) on dna synthesis of gene 46-47 mutants of bacteriophage t4d.	mutants in genes 46 and 47 of bacteriophage t4 exhibit early cessation of dna synthesis, inability to form a normal rapidly sedimenting dna intermediate (200s), reduced genetic recombination, and reduced viable phage production. a gene-specific suppressor mutation called das partially restores many of the pleiotropic effects of gene 46-47 mutants (13). our results indicate that this partial suppression by das is associated with (i) the synthesis of a small fraction of dna containing long single ...	1973	4583888
transforming ability of a t4 rna-dna copolymer.	a coliphage t4-specific rna-dna copolymer is genetically competent to transform a marker in gene 43, the dna polymerase.	1973	4583889
variable transforming activity of nonglucosylated and fragmented t4 dna.	nonglucosylated and fragmented t4 dna shows a gene-specific variation in transformation efficiency.	1973	4583890
an inhibitor of host sigma-stimulated core enzyme activity that purifies with dna-dependent rna polymerase of e. coli following t4 phage infection.		1973	4585685
role of t4 phage-directed protein in the establishment of resistance to t4 ghosts.		1973	4585893
sequence of t4, t2 and t6 bacteriophage species i rna and specific cleavage by an e. coli endonuclease.		1973	4586119
phleomycin-stimulated degradation of deoxyribonucleic acid in escherichia coli.	phleomycin stimulates the degradation of dna by energy-dependent endonuclease and exonuclease reactions in escherichia coli rec(+) cells and in recb(-) and recc(-) cells that lack an adenosine triphosphate-dependent nuclease functioning in the repair of ultraviolet (uv) lesions. exonuclease activity is blocked in t4 phage-infected cells. the endonuclease reaction produces 10(7)-dalton segments resembling those produced in colicin e2-treated cells. these differ from the random-sized segments prod ...	1973	4586146
rifampicin resistant dna synthesis in phage t4 infected escherichia coli.		1973	4586624
defective initiation of f2 rna translation by ribosomes from bacteriophage t4-infected cells.		1973	4586627
replication of t4 dna in escherichia coli treated with toluene.	deoxyribonucleoside triphosphates are incorporated into t4 dna in infected cells treated with toluene. under the proper conditions the incorporation is controlled by the known t4 dna polymerase and proceeds by a semiconservative mechanism. both strands of the phage dna are replicated into a high molecular weight progeny molecule. the replication system is accessible to extracellular pancreatic dnase added to the reaction mixture. at early times after infection a second replication system, not un ...	1973	4586770
synthesis of bacteriophage and host dna in toluene-treated cells prepared from t4-infected escherichia coli: role of bacteriophage gene d2a.	we investigated the synthesis of dna in toluene-treated cells prepared from escherichia coli infected with bacteriophage t4. if the phage carry certain rii deletion mutations, those which extend into the nearby d2a region, the following results are obtained: (i) phage dna synthesis occurs unless the phage carries certain dna-negative mutations; and (ii) host dna synthesis occurs even though the phage infection has already resulted in the cessation of host dna synthesis in vivo. the latter result ...	1973	4586772
template properties of glucose-deficient t-even bacteriophage dna.	the vegetative dna isolated from t4-infected escherichia coli w4597 (udpg ppase(-)) was about two to six times more active in stimulating protein synthesis in cell-free extracts than that isolated from t4-infected e. coli b06. this suggested that nonglucosylated vegetative dna may be a better template than the glucosylated form. this view was supported by experiments measuring rna synthesis on mature t-even dnas with a range of glucose contents. the extent of (14)c-gtp polymerization was inverse ...	1973	4586774
bacteriophage t4-directed dna synthesis in toluene-treated cells.	dna synthesis has been studied in t4-infected escherichia coli cells made permeable to nucleotides by treatment with toluene. the rate of incorporation of labeled deoxyribonucleoside triphosphates into dna at various times after infection is proportional to the in vivo rate. this in vitro incorporation is dependent on all four deoxyribonucleoside triphosphates (5-hydroxymethyldeoxy-cytidine triphosphate can substitute for dctp) and mg(2+). it is stimulated by ratp, partially inhibited by pancrea ...	1973	4586776
immediate-early expression of the gene causing superinfection breakdown in bacteriophage t4b.	superinfection breakdown appears to belong to the "immediate-early" functions induced by t4 phage.	1973	4586782
outer-membrane proteins induced by t4 bacteriophage.		1973	4587716
an electron microscopic analysis of pathways for bacteriophage t4 dna recombination.		1973	4588574
the identification of prereplicative bacteriophage t4 proteins.		1973	4588684