Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the nervous system in genital herpes simplex virus type 2 infections in mice. lethal panmyelitis or nonlethal demyelinative myelitis or meningitis. | female mice were inoculated vaginally with the ms strain of herpes simplex virus type 2, and serially positive vaginal cultures were used to confirm infection. the proportion of mice infected and the mortality rate in infected mice decreased with increasing age. in mice 12 weeks old, clinical, neuropathologic, and virologic criteria defined four patterns of disease. moribund mice had severe genital lesions, hindleg paralysis, and urinary and fecal retention, and most died during the second week ... | 1984 | 6092778 |
| characterisation of a herpes simplex virus type 1 mutant which has a temperature-sensitive defect in penetration of cells and assembly of capsids. | a herpes simplex virus type 1 (hsv-1) mutant, ts1204, which has a temperature-sensitive (ts) mutation located within genome map coordinates 0.318 to 0.324, close to but outside the coding sequences of the glycoprotein gb gene, has been characterised. although this mutant adsorbed to the cell surface at the nonpermissive temperature (npt), it failed to penetrate the cell membrane. as a consequence of this defect, high multiplicities of infection of ts1204 blocked subsequent infection of cells by ... | 1984 | 6093367 |
| interactions between herpes simplex virus dna-binding proteins. | monoclonal antibodies directed against several herpes simplex virus (hsv)-induced dna-binding proteins were used to investigate protein interactions in hsv-infected cells. q1 monoclonal antibody, which is specific for the hsv-induced alkaline nuclease, when used in an immunoadsorbant column resulted in the purification of the alkaline nuclease, to which large quantities of the major dna-binding protein were bound. conversely, when a monoclonal antibody to the major dna-binding protein was used i ... | 1984 | 6094715 |
| infection of murine hepatocyte cultures by herpes simplex virus (hsv) 1 and 2. | a study was undertaken of the interaction between liver cells and herpes simplex virus (hsv) in vitro. hepatocytes were obtained from hsv-resistant (c57/b16) and from hsv-susceptible (balb/c, a/j, c3h) mouse strains and cultured according to standard methods. each culture was infected with several strains of hsv-type 1 or of hsv-type 2, respectively. the multiplicity of infection was 5. the cytopathic effect was evaluated by light- and electron-microscopy. the number of infectious particles was ... | 1984 | 6094943 |
| herpes simplex virus latency in immunosuppressed mice. | to determine the role of antibodies in establishing herpes simplex virus (hsv) latency, immunosuppressed swiss mice were experimentally infected in the right hind footpad with a hsv-1 x hsv-2 recombinant (c6d) with low virulence. immunosuppression was induced by repeated intraperitoneal inoculations of cyclophosphamide (cy) and the production of antibodies to c6d in immunosuppressed animals was monitored by enzyme linked immunosorbent assay (elisa). within 21 days after inoculation, c6d was able ... | 1984 | 6094985 |
| [herpes simplex virus infections]. | there are two serological types of herpes simplex virus, hsv-1 and hsv-2. the types can be differentiated by different methods. after a primary infection hsv reaches the sensory ganglions by the way of peripheral nervous tracts. the virus remains for life in the ganglion as virus genome. after activation and return movement a new infection can appear. a lot of various diseases are caused by primary infection, reactivation or reinfection. the diagnosis in herpes virus diseases includes the detect ... | 1985 | 4039869 |
| viral meningitis. | viral meningitis is part of the aseptic meningitis syndrome but must be distinguished from bacterial meningitis on the basis of a careful examination of the csf and sound clinical judgment. enteroviruses probably account for the bulk of cases of aseptic meningitis that occur in the united states and which are reported to the centers for disease control each year. the seasonal pattern in the incidence of aseptic meningitis is largely due to the seasonal variation of enteroviral infections. early ... | 1985 | 3990441 |
| degradation of cellular mrnas induced by a virion-associated factor during herpes simplex virus infection of vero cells. | we have used northern blot hybridization to study the accumulation of specific cellular mrnas in vero cells infected with herpes simplex virus (hsv) type 1 or type 2. hsv-1 infection decreased the cytoplasmic levels of beta- and gamma-actin, beta-tubulin, and histone h3 and h4 mrnas, though not all at the same rate. hsv-2 infection resulted in a more rapid decrease in actin and histone mrna levels compared with hsv-1 infection. the turnover rate of each type of mrna studied was accelerated in hs ... | 1985 | 4020960 |
| the effect of dimethylnitrosamine on host resistance and immunity. | adult female b6c3f1 mice were injected ip with 0.2 ml phosphate-buffered saline (pbs) only or pbs containing 1.5, 3, or 5 mg dimethylnitrosamine (dmn)/kg body wt daily for 14 days. on day 16, mice were evaluated for changes in immune status as measured by the antibody response to sheep red blood cells (srbcs), blastogenesis to t- and b-cell mitogens, natural killer (nk) cell function, delayed hypersensitivity, and alveolar macrophage (am) bactericidal activity; and for changes in host resistance ... | 1985 | 3883572 |
| anti-herpes simplex virus and cytostatic activity of some new 5-substituted 1-(4-hydroxybutyl)-and 1-(2-hydroxyethoxymethyl) uracil nucleoside analogues. | two series of 5-substituted acyclic uracil nucleoside analogues (5-x-acyclo-u) were evaluated for their inhibitory effects against three herpes simplex virus type 1 (hsv-1) strains and one type 2 (hsv-2) strain in a plaque inhibition assay on human embryonic lung fibroblast (helf) cell cultures as well as for their ability to inhibit the proliferation of baby hamster kidney cells in suspension (bhk-s) culture. acyclovir [9-(2-hydroxyethoxymethyl)guanine; acv] and (s)-9-(2,3-dihydroxypropyl)adeni ... | 1985 | 2864819 |
| herpes simplex virus hepatitis in mice: effects of treatment with trisodium phosphonoformate. | clinical isolates of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) induced focal necrotic hepatitis in icr/schö mice. the extent of the hepatitis could be considerably reduced by trisodium phosphonoformate (pfa) administered for 3 days in a daily dose of 400-600 mg/kg. the hsv hepatitis in mice could serve as a suitable model for testing antiherpetic drugs. | 1985 | 2869661 |
| multistep transformation by defined fragments of herpes simplex virus type 2 dna: oncogenic region and its gene product. | diploid syrian hamster embryo cells transfected with bgl ii c fragment of herpes simplex virus type 2 dna acquired a neoplastic phenotype. cultures transfected with its left-hand 64% subclone ecori/hindiii fragment ae (0.419-0.525 map unit) grew into established but nontumorigenic lines. transfection of ecori/hindiii ae-immortalized cells with a 4.4-kilobase sac i/bamhi subfragment within bamhi e (0.554-0.584 map unit; overlaps the right-hand 16% of bgl ii c) converted them to tumorigenicity. th ... | 1985 | 3001705 |
| risk of recurrence after treatment of first-episode genital herpes with intravenous acyclovir. | to determine whether intravenous acyclovir treatment for a first episode of genital herpes could prevent or reduce subsequent recurrences, we combined and analyzed the results of two independently conducted, randomized, double-blind, placebo-controlled studies. sixty-one patients were enrolled in the two trials; 30 received the drug, and 31 received placebo. at entry the demographic, epidemiologic, and clinical features of acyclovir- and placebo-treated patients from the two centers showed no si ... | 1985 | 3001952 |
| herpes simplex virus antigen detection in human acute encephalitis: an immunohistochemical study using avidin-biotin-peroxidase complex method. | autopsy specimens from six patients with clinically diagnosed herpes simplex virus (hsv) encephalitis were studied. since immunocytochemistry has been reported to be a more reliable and successful method to identify hsv as the etiologic agent, antitype 1 hsv (hsv1) and antitype 2 hsv (hsv2) and the avidin-biotin-peroxidase complex (abc) method were applied to brain paraffin sections. positive immunostaining was observed in front-orbital, mediobasal temporal lobes, cingulate gyrus, and insula. th ... | 1985 | 3002087 |
| [inhibition of virus multiplication by immunoactive peptides]. | in the present study we show that peritoneal macrophages obtained from the mice treated with the immunoactive peptides inhibit the multiplication of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), but not that of vesicular stomatitis virus (vsv), and that the intraperitoneal administration of the peptides suppresses the infection with hsv-1 in mice. | 1985 | 3002570 |
| investigation of the virulence genes of herpes simplex virus 2 by experimental infection in vivo with defined intertypic recombinants of a virulent hsv-2 x an avirulent hsv-1. | for determination of the gene loci for virulence and tropism of herpes simplex virus 2 (hsv-2) intertypic recombinants of hsv-1 strain hfem and hsv-2 strain 3345 were screened for their pathogenicity in the tree shrew. hsv-1 strain hfem is not pathogenic in the tree shrew and can be recovered as a latent virus only from the spleen of the animals; but hsv-2 strain 3345, the other parental virus of the recombinants, was found to be virulent for the tree shrew and colonized the ganglia of latently ... | 1985 | 3003551 |
| [herpes simplex virus type 2 infection. etiology, clinical aspects, diagnosis, therapy, hsv-2 and cervix cancer]. | the genital herpes infection is mostly caused by hsv-2. the analysis of epidemic data supports the common impression that herpes genitalis markedly increased. the hsv-2 is with few exceptions a sexually transmitted virus, the infectionsity is about 80%. the primary infection with hsv-2 occurs in the youth. hsv-2 survives in a latent stage in the human body for life and causes as a rule to recurrent diseases. the clinical picture is variable. the most severe complication is the sepsis of newborn. ... | 1985 | 3004067 |
| cellular proteins expressed in herpes simplex virus transformed cells also accumulate on herpes simplex virus infection. | the cell proteins expressed in rat embryo cells transformed by herpes simplex virus (hsv) have been analysed by immunoprecipitation assays to determine those polypeptides which can be identified by immunoprecipitation with the sera of tumour-bearing animals and also with antisera to herpes simplex infected cells. cell polypeptides commonly recognised by both these sera have been further characterised using a monoclonal antibody directed against a cellular polypeptide which accumulates on hsv-2 l ... | 1985 | 3004943 |
| retention of herpes simplex virus type ii sequences in bglii n-transformed cells after co-transfection with a selectable marker. | transformation of mammalian cells by total u.v.-inactivated herpes simplex virus ii (hsvii) or cloned fragments thereof (bglii n, bglii c) has been complicated both by a low efficiency of oncogenic transformation and the disappearance of viral dna and/or viral products initially detected in the transformed cell lines. in an attempt to effect a stable integration of bglii n and to elucidate the role of hsvii in oncogenic transformation, we have co-transfected nih 3t3 cells with pag60, a plasmid w ... | 1985 | 3004960 |
| a close association of adriamycin resistance with expression of cell surface antigens in adriamycin-resistant cell lines of herpes simplex virus type 2-transformed syrian hamster cells. | we determined the properties of three adriamycin (adm)-resistant cell lines isolated from a clone of herpes simplex virus (hsv) type 2-transformed syrian hamster cells. the adm-resistant lines (admr-6, -7 and -9) established by continuous exposure of the cells to adm were 20 to 30 times more resistant to adm than was the parent line. these resistant lines exhibited cross-resistance to daunomycin, actinomycin d, chromomycin a3 and vincristine, but not to mitomycin c, bleomycin or methotrexate. up ... | 1985 | 3005209 |
| acyclovir prophylaxis in bone marrow transplant recipients. | forty-two patients undergoing bone marrow transplantation were included in a randomised, double-blind and placebo controlled trial of prolonged acyclovir prophylaxis against infections with viruses of the herpes group. twenty patients were allocated to receive acyclovir and 22 to receive placebo. acyclovir or placebo was administered i.v. at a dose of 250 mg/m2 twice daily, starting 5 days before transplantation. at 5 weeks after transplantation, administration was changed to tablets, 400 mg thr ... | 1985 | 3006228 |
| virological aspects on herpesvirus infections. | the family herpesviridae include five important human pathogens, herpes simplex virus types 1 and 2 (hsv-1, hsv-2), varicella-zoster virus (vzv) and cytomegalovirus (cmv). the five viruses are represented in all three herpesvirus subfamilies, a subdivision based on differences in phenotypic properties such as length of replication cycle, cytotoxic properties and host cell range. the herpesviruses have dna genomes sharing characteristic anatomical features. they replicate in the nuclei of eukaryo ... | 1985 | 3006235 |
| [isolation of an endogenous guinea pig herpeslike virus from hartley guinea pigs with latent herpes simplex virus type 2 infection]. | 1985 | 3017012 | |
| plaque autoradiography assay for the detection and quantitation of thymidine kinase-deficient and thymidine kinase-altered mutants of herpes simplex virus in clinical isolates. | a plaque autoradiography assay to detect and quantitate thymidine kinase (tk) mutants of herpes simplex virus type 1 (hsv-1) and hsv-2 in clinical samples is described. this method utilizes the selective incorporation of [125i]iododeoxycytidine, a pyrimidine analog selectively phosphorylated by the hsv tk. only cells infected with tk-competent virus will efficiently incorporate iododeoxycytidine and are the only cells detected by autoradiography. furthermore, this assay discriminates between tk+ ... | 1985 | 3010836 |
| selective in vitro and in vivo activities of 5-(2-haloalkyl)pyrimidine nucleoside analogs, particularly 5-(2-chloroethyl)-2'-deoxyuridine, against herpes simplex virus. | 5-(2-chloroethyl)-2'-deoxyuridine (cedu), 5-(3-chloropropyl)-2'-deoxyuridine (cpdu), and 5-(2-chloroethyl)-2'-deoxycytidine (cedc) were evaluated for activity against herpes simplex virus type 1 (hsv-1) and hsv-2 in vitro. their mics for hsv-1 in primary rabbit kidney cell cultures were 0.15, 0.20, and 0.60 micrograms/ml, respectively; their mics for hsv-2 were about 10-fold higher. when tested in parallel, the reference compounds 5-ethyl-2'-deoxyuridine, 5-iodo-2'-deoxyuridine, acyclovir, and ( ... | 1985 | 3010841 |
| mechanism of selective inhibition of human cytomegalovirus replication by 1-beta-d-arabinofuranosyl-5-fluorouracil. | four kinds of 1-beta-d-arabinofuranosyl-5-halogenouracil were examined for inhibition of human cytomegalovirus (hcmv) and herpes simplex virus type 1 (hsv-1) and 2 (hsv-2) replication. 1-beta-d-arabinofuranosyl-5-fluorouracil (ara-fu) was the most effective against hcmv, whereas 1-beta-d-arabinofuranosyl-5-bromouracil was the most effective against hsv-1 and hsv-2. the mechanism of action of ara-fu on hcmv replication was also studied. the dttp pool size in human embryonic fibroblasts was increa ... | 1985 | 3010844 |
| [demonstration of latent infection by herpes simplex virus type 2 (hsv-2) in the spinal cord of the guinea pig]. | 1985 | 3008964 | |
| [inhibitory effect of tai-ding-an on dna and protein synthesis of herpes simplex virus type 2 (hsv-2)]. | 1985 | 3008965 | |
| [discovery of herpes simplex virus (hsv)-like particles in biopsy samples of cervical carcinoma]. | the tissue of cervical cancer and hsv-2 infected baby rabbit kidney (brk) cells were studied comparatively by electron microscope. in 2 out of 12 samples from patients with cervical carcinoma, some virus-like particles were observed in the cytoplasm or intercellular space. some particles seemed to be enveloped by endoplasmic reticulum. according to their size, structure and morphology, these particles resembled the herpes simplex virus. the relationship between the discovery of herpes simplex vi ... | 1985 | 3015529 |
| production of lymphokines and interferon by immune cells involved in recovery of mice from herpes simplex virus type 2 hepatitis. | adoptive transfer of spleen cells from mice 4 days after infection with herpes simplex virus type 2 (hsv-2) reduced the virus titer in the liver of recipient mice infected 24 h before transfer. macrophage chemotactic factor (cf) and macrophage migration inhibition factor (mif) were produced by day 3 of infection in spleen cell cultures stimulated with hsv-2, but not with control antigen, i.e. 1 day before the cells are active in adoptive transfer. interferon was produced in cultures established ... | 1985 | 2408997 |
| studies on the herpes simplex virus alkaline nuclease: detection of type-common and type-specific epitopes on the enzyme. | five monoclonal antibodies to the alkaline nuclease of herpes simplex virus (hsv) types 1 and 2 have been used in immunoperoxidase tests to demonstrate the nuclear localization of the enzyme within hsv-1- and hsv-2-infected cells and to purify the enzyme from cells infected with either virus by immunoadsorbant chromatography. affinity chromatography with a 32p-labelled extract of hsv-2-infected cells has enabled us to demonstrate that the nuclease eluting from the immunoadsorbant is a phosphopro ... | 1985 | 2578550 |
| dna-binding protein associated with herpes simplex virus dna polymerase. | purified preparations of herpes simplex virus type 2 dna polymerase made by many different laboratories always contain at least two polypeptides. the major one, of about 150,000 molecular weight, has been associated with the polymerase activity. the second protein, of about 54,000 molecular weight, which we previously designated icsp 34, 35, has now been purified. the purified protein has been used to prepare antisera (both polyclonal rabbit serum and monoclonal antibodies). these reagents have ... | 1985 | 2578573 |
| human cytotoxic t cell clones directed against herpes simplex virus-infected cells. iii. analysis of viral glycoproteins recognized by ctl clones by using recombinant herpes simplex viruses. | human cytotoxic t cell (ctl) clones specific for herpes simplex virus (hsv) type 1- and type 2-infected cells were generated and were analyzed with regard to the viral glycoproteins they recognize on autologous hsv-infected cells. by use of target cells infected with wild-type hsv strains, a gc deletion mutant of hsv-1, and hsv-1 x hsv-2 intertypic recombinants, some hsv-1-specific ctl clones were found to be directed against l region-encoded ga/b-1, and others against s region-encoded glycoprot ... | 1985 | 2579149 |
| frequency of herpes simplex virus-specific helper t lymphocyte precursors in the lymph node cells of infected mice. | we have established a limited dilution assay to estimate the frequency of herpes simplex virus type 1 (hsv)-specific, interleukin 2 (il 2)-producing helper t lymphocyte precursors (htl-p). the estimated frequency of such cells in suspensions of local lymph node (ln) cells 5 days after in vivo virus infection was 1:2470 to 1:5800. frequencies of htl-p in cells from uninfected mice were below levels of detection of our system and were judged to be below 1:100,000. removal of lyt-2+ cells from resp ... | 1985 | 2579150 |
| antibody response to type-common and type-unique epitopes of herpes simplex virus polypeptides. | herpes simplex virus type 1 (hsv1) and type 2 (hsv2) polypeptides with type-common and type-unique epitopes were identified using cross-adsorbed hyperimmune rabbit sera and western blotting techniques. twelve hsv1 and fourteen hsv2 polypeptides with type-specific epitopes were identified. cross-adsorbed human sera reacted to a subset of the type-specific epitopes defined by rabbit sera. human sera with both hsv1- and hsv2-specific antibodies were identified by their reaction to hsv1-type-specifi ... | 1985 | 2580054 |
| rapid detection of cytomegalovirus in cell culture by indirect immunoperoxidase staining with monoclonal antibody to an early nuclear antigen. | a method for the rapid detection of cytomegalovirus (cmv) in mrc-5 cells 48 h after inoculation with clinical specimens was developed. a commercially available monoclonal antibody to a cmv early nuclear antigen was used in an indirect immunoperoxidase (ipa) staining procedure performed directly on acetone-fixed cell monolayers in standard tubes (16 by 125 mm). of 190 clinical specimens tested, 30 specimens produced cmv cytopathic effect in tissue culture (tc-cpe) within 14 days after inoculation ... | 1985 | 2581991 |
| detection of herpes simplex virus type-2 antigen(s) in biopsies from carcinoma of the uterine cervix. | indirect immunofluorescence technique was employed to detect herpes simplex virus type-2 (hsv-2) antigens in tumor biopsies from 215 patients with carcinoma of the uterine cervix. a total of 169 samples (79%) revealed brilliant nuclear fluorescence. inflammatory cells infiltrating the tumor mass were positive to 60 of the 215 patients (28%). samples showed no significant variation in the degree of fluorescence or proportion of cells binding hsv antibody with advancement in the clinical stage of ... | 1985 | 2431113 |
| a prospective study of association of herpes simplex virus and human papillomavirus infection with cervical neoplasia in women exposed to diethylstilbestrol in utero. | one thousand one hundred thirty-four women, enrolled in a prospective study of the development of cervical neoplasia in women exposed to diethylstilbestrol (des) in utero, were examined at least once a year. twenty-three women developed cervical intraepithelial neoplasia (cin) of different degrees during the 7-year follow-up period and were matched with women who did not develop cin during the study period. serum samples were obtained at the time of entry into the study and at the time of diagno ... | 1985 | 2981778 |
| herpes simplex virus type 2 and human papillomavirus type 16 in cervicitis, dysplasia and invasive cervical carcinoma. | sera and biopsies of cervical lesions from 55 women with benign or malignant disease were analyzed for evidence of infection with herpes simplex virus type 2 (hsv-2) or human papillomavirus (hpv). in addition, information regarding known risk factors for cervical cancer was obtained by interview. the sera were tested for hsv-2 antibodies and the biopsies were tested for hpv or hsv dna sequences by southern blot hybridization. hsv-2 sequences were detected in 2/13 (15%) invasive neoplasms and in ... | 1985 | 2981783 |
| antigenic and biochemical analysis of gb of herpes simplex virus type 1 and type 2 and of cross-reacting glycoproteins induced by bovine mammillitis virus and equine herpesvirus type 1. | an antiserum was produced to the oligomeric form of glycoprotein b (gb) induced by herpes simplex virus type 1 (hsv-1) strain 17. this antiserum gave a single common precipitin line in agar gel immunodiffusion with hsv-1, hsv-2, bovine mammillitis virus (bmv) and equine herpesvirus type 1 (ehv-1). it also neutralized hsv-1, hsv-2 and bmv but not ehv-1. absorption of the antiserum with excess hsv-2 or bmv antigen resulted in an hsv-1-specific neutralizing antiserum. in immunoprecipitation, two pr ... | 1985 | 2981966 |
| increased mutation frequency after herpes simplex virus type 2 infection in non-permissive xc cells. | the effect of herpes simplex virus type 2 (hsv-2) infection on the frequency of mutations at the hypoxanthine-guanine phosphoribosyl transferase (hgprt) locus was studied in the non-permissive xc cell line. when the cells were infected with 20 to 800 p.f.u./cell, there was initially a lag in cell growth and cell death, but after 4 days there was no difference in growth rate between infected and control cultures. however, the mutation frequency, as determined by the number of 6-thioguanine-resist ... | 1985 | 2981968 |
| characterization of the gene encoding herpes simplex virus type 2 glycoprotein c and comparison with the type 1 counterpart. | the gene encoding the glycoprotein c (gc) of herpes simplex virus type 1 maps to the region of the viral genome from 0.62 to 0.64. recently, a herpes simplex virus type 2 glycoprotein previously designated gf and now designated gc was mapped to a homologous location. analysis of the herpes simplex virus type 2 mrna species encoded in this region revealed a major transcript of 2.5 kilobases, a 0.73-kilobase transcript (the 5' ends of which were mapped by primer extension), and several minor speci ... | 1985 | 2982036 |
| herpes simplex virus latency in a hyperresistant clone of mouse neuroblastoma (cl300) cells. | herpes simplex virus (hsv) hyperresistant neuroblastoma cells (clone cl300 rii) were latently infected with hsv-1 if cultured in presence of hsv-neutralizing antibody for one or two passages after infection and then passaged further without antibody. by superinfecting hsv-1 latently infected cl300 rii cells with hsv-2, progeny virus with hsv-1 characteristics was regularly rescued. such retrieval of hsv-1 decreased with passage of the latently infected cells. | 1985 | 2982359 |
| antiviral activity of cp-20,961 against herpes simplex viruses in vitro. | the antiherpesvirus activity of cp-20,961 [n,n-dioctadecyl-n',n'-bis (2-hydroxyethyl) propanediamine, or avridine] was investigated in cultured guinea pig embryo (gpe) cells. plaque formation of herpes simplex virus type 1 (hsv1) and type 2 (hsv2) was inhibited, but vesicular stomatitis virus replication was not inhibited, in gpe cells treated with cp-20,961 before infection. the id50 concentration of cp-20,961 for hsv was about 50 micrograms/ml for 3-4 days of pretreatment. after virus adsorpti ... | 1985 | 2982759 |
| herpes simplex virus type 1 infection of endothelial, epithelial, and fibroblast cells induces a receptor for c3b. | we recently demonstrated that herpes simplex virus type 1 (hsv 1) induces a receptor on human umbilical vein endothelial cells for complement component c3b (c3br). we assigned this receptor function to hsv 1 viral glycoprotein c (gc) based on several observations: tunicamycin, which prevents glycosylation and expression of n-linked glycoproteins on the surface of infected cells, markedly reduced expression of the c3br; monoclonal antibodies to hsv 1 gc blocked detection of the c3br, whereas mono ... | 1985 | 2982950 |
| acute and latent infection by thymidine kinase mutants of herpes simplex virus type 2. | 1985 | 2982968 | |
| herpes simplex virus sequences involved in the initiation of oncogenic morphological transformation of rat cells are not required for maintenance of the transformed state. | we have determined the herpes simplex virus (hsv) type 2 dna sequences responsible for the initiation of morphological transformation and have investigated the retention and expression of these sequences in morphologically transformed cells and in tumours derived from these cells. all the transformed cells analysed were selected by a focus formation assay and are oncogenic in the inbred host rat. cloned hindiii and bg/ii fragments from the hsv-2 genome were assayed for the ability to initiate mo ... | 1985 | 2983000 |
| evidence for a direct role for both the 175,000- and 110,000-molecular-weight immediate-early proteins of herpes simplex virus in the transactivation of delayed-early promoters. | we reconstructed the regulated induction of delayed-early (de) transcription that occurs during herpes simplex virus (hsv) infection by using a transient expression system in which recombinant target genes were cotransfected into vero cells together with intact activating genes. plasmids containing cloned hsv-1 or hsv-2 immediate-early (ie) genes stimulated by up to 100-fold the expression from recombinant constructs containing the bacterial chloramphenicol acetyltransferase (cat) gene under the ... | 1985 | 2983086 |
| the herpes simplex virus amplicon: analyses of cis-acting replication functions. | previous studies have shown that defective virus vectors (amplicons) derived from herpes simplex viruses could be efficiently propagated in virus stocks in the presence of trans-acting helper virus functions. the present study established that two separate cis-acting functions--a dna replication origin and a cleavage/packaging signal--are required for amplicon propagation. using deleted derivatives of cloned amplicons, we mapped one of the viral dna replication origins (ori-2 or oril) at coordin ... | 1985 | 2983310 |
| protection from genital herpes simplex virus type 2 infection by vaccination with cloned type 1 glycoprotein d. | guinea pigs were vaccinated with truncated herpes simplex virus type-1 (hsv-1) glycoprotein d produced in the genetically engineered mammalian cell line gd10.2. vaccinated animals formed antibodies that neutralized both hsv-1 and herpes simplex virus type 2 (hsv-2) in an in vitro neutralization assay. vaccinated animals were challenged with hsv-2 by intravaginal infection. animals that received the immunogen in freund's complete adjuvant were completely protected from the clinical manifestations ... | 1985 | 2983428 |
| a prospective study of herpes simplex virus infection in a defined population in houston, texas. | a seroepidemiologic study was conducted to determine the incidence of antibodies to herpes simplex virus in a population of middle-class caucasian women. in utero exposure to diethylstilbestrol did not influence the frequency of herpes simplex virus infection. the frequency of infection increased with age and number of sex partners and a 50% increase in incidence of herpes simplex virus type 2 infection was noted during the 4-year period of the study. subclinical herpes simplex virus type 2 infe ... | 1985 | 2983557 |
| herpes simplex virus type 2 encephalitis in two homosexual men with persistent lymphadenopathy. | within a 5-month period, 2 homosexual men with persistent lymphadenopathy developed clinical findings consistent with herpes simplex virus (hsv) encephalitis. these signs included abrupt change in mental status, seizures, cerebrospinal fluid pleocytosis, and localized electroencephalographic abnormalities showing temporal lobe involvement. initial computed tomographic scans were unremarkable. treatment with adenine arabinoside was instituted and temporal lobe biopsies were performed. although vi ... | 1985 | 2983602 |
| can ribonucleotide reductase be considered as an effective target for developing antiherpes simplex virus type ii (hsv-2) compounds? | herpes simplex viruses are known to induce virus specified ribonucleotide reductase (rr) in infected cells. rr is considered as a possible target for the development of antiviral agents. in this study, the role of rr in virus replication has been investigated. the sensitivity of rr to hydroxyurea (hu) from virus infected and uninfected hela s3 cells was similar with ic50 values of 0.12 and 0.14 mm. in the presence of 2 mm hu, and 10 microm tetrahydrouridine (thu), a cytidine deaminase inhibitor, ... | 1985 | 2983736 |
| microcarriers in combination with enzyme immunofiltration and immunofluroescence for the detection of herpes simplex virus antigens in culture. | a microcarrier culture system in combination with enzyme immunofiltration with a herpes simplex virus (hsv) group monoclonal antibody was found to be as sensitive as immunofluorescence for the detection of hsv type 1 (hsv-1) in cell cultures and to give specific identification at the same time as the appearance of the cytopathic effect with very high infectious inocula and within 10 to 24 h of the appearance of the cytopathic effect with very high infectious inocula and within 10 to 24 h of the ... | 1985 | 2984242 |
| problems associated with the use of (e)-5-(2-bromovinyl)-2'-deoxyuridine for typing herpes simplex virus. | when 0.5 microgram of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) per ml was incorporated directly into cell culture medium inoculated with eight known positive specimens, one herpes simplex virus type 1 (hsv-1) isolate grew in the presence of bvdu and was misidentified. by plaque assay, the titers of 15 hsv-1 strains were reduced by more than 3 log10 by bvdu, and the titers of 16 hsv-2 strains were reduced by less than 2 log10. titers of hsv-1 acyclovir-resistant strains were reduced by less th ... | 1985 | 2984249 |
| identification and typing of herpes simplex virus types 1 and 2 by monoclonal antibodies, sensitivity to the drug (e)-5-(2-bromovinyl)-2'-deoxyuridine, and restriction endonuclease analysis of viral dna. | with development of antiviral drugs, the need to identify a virus as to drug sensitivity becomes increasingly of importance. the compound (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) has been shown to be much more inhibitory to the replication of herpes simplex virus type 1 (hsv-1) and varicella-zoster virus as opposed to herpes simplex virus type 2 (hsv-2). we have typed over 170 isolates, using an immunofluorescent technique and sensitivity to the drug bvdu. these results were then compared to ... | 1985 | 2984324 |
| comparison of western blot analysis to microneutralization for the detection of type-specific herpes simplex virus antibodies. | a newly developed system (as described in the previous paper) employing separated herpes simplex virus (hsv) polypeptides electrophoretically transferred to nitrocellulose paper, western blot analysis (wba), was compared to microneutralization (mn) for the detection of type-specific antibody to hsv types 1 and 2 in 98 human sera. sera containing hsv1-specific antibodies reacted at least twofold more densely (as quantitated by densitometric scanning) to hsv1 than hsv2 polypeptides, while sera wit ... | 1985 | 2984325 |
| synthesis and antitumor and antiviral activities of a series of 1-beta-d-ribofuranosyl-5-halocytosine (5-halocytidine) cyclic 3',5'-monophosphates. | a series of 1-beta-ribofuranosyl-5-halocytosine cyclic 3',5'-monophosphates (1-4) has been prepared. direct halogenation of cytidine 3',5'-monophosphate (ccmp) yielded the cl, br, and i compounds while 5-f-ccmp (1) was obtained on cyclization of the 5'-monophosphate. on in vitro testing of 1-4 against l1210 and p388 leukemias, only 1 showed significant low-level activity (id50 = 3.1 x 10(-4) mmol/l). derivatives 2-4 were inactive at 10(-1) mmol/l and also proved to have low viral ratings against ... | 1985 | 2984417 |
| protection of mice against fatal herpes simplex type 2 infection by liposomes containing muramyl tripeptide. | intravenous administration of liposomes containing muramyl tripeptide phosphatidylethanolamine, a lipophilic derivative of muramyl dipeptide that activates macrophages to a cytolytic state in situ, significantly protected mice against lethal challenge with herpes simplex virus type 2. these findings suggest that the systemic activation of macrophages by liposomes containing an immunomodulator can lead to prophylaxis of severe infections caused by herpesviruses. | 1985 | 2984772 |
| oral acyclovir therapy of genital herpes simplex virus type 2 infections in guinea pigs. | oral acyclovir was evaluated for its effectiveness in treating guinea pigs with primary herpes simplex virus type 2 infections. guinea pigs inoculated intravaginally with acyclovir-susceptible strains (for which 50% inhibitory concentrations of acyclovir in cell culture were found to be in the range of 0.15 to 1.2 micrograms/ml) and treated with 5.0 mg of acyclovir per ml in the drinking water beginning 48 h postinfection showed significant reductions in lesion severity. this dosage produced ser ... | 1985 | 2984984 |
| combination chemotherapy: interaction of 5-methoxymethyldeoxyuridine with trifluorothymidine, phosphonoformate and acycloguanosine against herpes simplex viruses. | methoxymethyldeoxyuridine (mmudr) when used in combination with either trifluorothymidine (f3tdr) or phosphonoformate (pfa) showed synergistic activity against herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) in vitro, whereas mmudr and acycloguanosine (acg) combination was antagonistic against herpes viruses. hsv-1 mutants resistant to acg, arabinofuranosyladenine (ara-a), mmudr or pfa were isolated. drug-resistant hsv-1 virus mutants were analyzed for cross sensitivity to acg, ara-a, f ... | 1985 | 2984988 |
| enzyme-linked immunosorbent assay for determination of antibodies against herpes simplex virus types 1 and 2 in human sera. | a rapid and reproducible enzyme-linked immunosorbent assay (elisa) is described for determining antibodies in human sera against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). the sera were absorbed for 30 min with heterologous virus-infected-cell extracts to remove cross-reacting antibodies and then were applied to elisa plates containing the target antigens, immunoaffinity-purified hsv-1 glycoproteins gc and gd and hsv-2 glycoproteins gd and gf. the absorbance index, defined as the ra ... | 1985 | 2985643 |
| evaluation of two immunofluorescence assays with monoclonal antibodies for typing of herpes simplex virus. | an indirect immunofluorescence assay and a direct immunofluorescence assay were evaluated for typing clinical isolates of herpes simplex virus (hsv). the indirect immunofluorescence assay (electro-nucleonics, inc.) correctly identified 16 hsv type 2 (hsv-2) isolates, but failed to identify 4 of 14 hsv-1 isolates because of background fluorescence and instability of reagents. forty-nine hsv-1 isolates were correctly typed by direct immunofluorescence assay (kallestad laboratories, inc.), but 1 of ... | 1985 | 2985651 |
| direct inactivation of viruses by mcp-1 and mcp-2, natural peptide antibiotics from rabbit leukocytes. | six homologous peptides were purified to homogeneity from rabbit granulocytes or alveolar macrophages and tested for their ability to inactivate herpes simplex virus type 1 (hsv-1). two of the peptides, mcp-1 and mcp-2, showed considerable in vitro neutralizing activity, whereas four structurally homologous peptides (np-3a, np-3b, np-4, and np-5) were relatively ineffective. inactivation of hsv-1 by mcp-1 or mcp-2 depended on peptide concentration and on the time, temperature, and ph of the incu ... | 1985 | 2985808 |
| vaccinia virus recombinant expressing herpes simplex virus type 1 glycoprotein d prevents latent herpes in mice. | in humans, herpes simplex virus causes a primary infection and then often a latent ganglionic infection that persists for life. because these latent infections can recur periodically, vaccines are needed that can protect against both primary and latent herpes simplex infections. infectious vaccinia virus recombinants that contain the herpes simplex virus type 1 (hsv-1) glycoprotein d gene under control of defined early or late vaccinia virus promoters were constructed. tissue culture cells infec ... | 1985 | 2986288 |
| [prevention and therapy of herpesvirus infections]. | the group of the human-pathogenic herpesviruses comprises five subgroups: herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2), varicella zoster virus (vzv), cytomegalovirus (cmv), and epstein-barr virus (ebv). primary infection with these ubiquitous herpesviruses usually occurs in childhood or during adolescence and frequently remains inapparent. however, it can also give rise to a variety of clinical pictures. important clinical manifestations of herpesvirus infections are ... | 1985 | 2986378 |
| synergism between recombinant human interferon and nucleoside antiviral agents against herpes simplex virus: examination with an automated microtiter plate assay. | an automated, quantitative, cytopathic effect (cpe) inhibition assay with human fibroblasts in 96-well microtiter plates was used to examine the combination of recombinant human interferon-alpha (rifn-alpha a) and acyclovir, vidarabine, or dihydroxypropoxymethyl guanine against herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) in vitro. fifty percent cpe (cpe50) end points, calculated from optical density readings of crystal violet-stained monolayers in an automated spectrophotometer, represent ... | 1985 | 2987369 |
| intracellular transport of herpes simplex virus gd occurs more rapidly in uninfected cells than in infected cells. | a mouse l cell line which expresses the herpex simplex virus type 1 immediate-early polypeptides icp4 and icp47 was cotransfected with a cloned copy of the bglii l fragment of herpes simplex virus type 2, which includes the gene for gd, and the plasmid psv2neo, which contains the aminoglycosyl 3'-phosphotransferase (agpt) gene conferring resistance to the antibiotic g418. a g418-resistant transformed cell line was isolated which expressed herpes simplex virus type 2 gd at higher levels than were ... | 1985 | 2987522 |
| synthesis and processing of glycoprotein gg of herpes simplex virus type 2. | monoclonal antibody 13 alpha c5-1-a11 immunoprecipitated two major polypeptides of molecular weights 108,000 and 120,000 from extracts of herpes simplex virus type 2-infected bhk-21 cells labeled with [35s]methionine or [3h]glucosamine. in pulse-chase experiments, both labels were chased from the 120,000-molecular-weight peptide (120k peptide) into the 108k molecule. endoglycosidase h (endo h) reduced the 120k peptide to a 112k peptide but did not affect the 108k peptide. similar profiles were o ... | 1985 | 2987534 |
| the consensus sequence ygtgttyy located downstream from the aataaa signal is required for efficient formation of mrna 3' termini. | our previous dna sequence comparisons of 3' terminal portions from equivalent herpes simplex virus type 1 (hsv-1) and hsv-2 genes identified a conserved sequence (consensus ygtgttyy; y = pyrimidine) located approximately 30bp downstream from the aataaa signal. we report here that this signal is located downstream from 67% of the mammalian mrna 3' termini examined. using constructions with the bacterial chloramphenicol acetyl transferase (cat) gene linked to an hsv 'terminator' fragment, we show ... | 1985 | 2987822 |
| prevalence of serum antibodies to herpes simplex virus types 1 and 2: application of an elisa technique to 100 cases of anogenital herpes. | sera of 100 patients affected by anogenital herpes were tested by an enzyme-linked immunosorbent assay (elisa) technique using partially purified antigens obtained by extraction from the nuclei of infected vero cells. herpes simplex virus type 1 (hsv-1) was isolated from the anogenital lesions of 17 patients and herpes simplex virus type 2 (hsv-2) from 83 patients. the relative proportions of antibody of the igg class to hsv-1 and hsv-2 were determined and their ratio (r) was calculated, except ... | 1985 | 2988144 |
| immunologic characterization of herpes simplex virus type 2 antigens icp10 and icsp11/12. | infected cell protein 10 (icp10) or antigen 4 (ag4) and infected cell-specific protein 11/12 (icsp11/12) have been suggested as specific antigenic markers for cervical carcinoma. experiments were designed to determine whether icp10 and icsp11/12 are distinct antigens and to determine the cellular localization of icp10. results indicate that an apparent 160 kdalton (kda) protein analyzed by 8.5% polyacrylamide gels (= 144 kda protein analyzed by 7.0% polyacrylamide gels) was detected in hsv-2-inf ... | 1985 | 2988230 |
| development of molecular probes for simian herpesvirus detection. | a number of serologic procedures are available for specifically determining past infection due to h. hominis, or h. simiae (b virus). a herpesvirus isolated from primate tissues, however, does not lend itself to specific identification. procedures currently in routine use simply do not provide an unequivocal identification of the isolate. we have previously demonstrated that h. simiae contain polypeptide and glycoprotein antigens distinct from those of other herpesviruses. at selected intervals, ... | 1985 | 2989049 |
| isolation and characterization of deletion mutants of herpes simplex virus type 2 (strain hg52). | we provide evidence that: (i) two variants lacking the xbai site at map coordinate 0.7 have been selected following xbai treatment of the dna of herpes simplex virus type 2 strain hg52; (ii) one of these mutants had lost the 0.7 restriction site due to a deletion of approximately 150 base pairs and in the other the site loss was due to a similar sized sequence insertion; (iii) following xbai treatment, four variants with deletions ranging in size from 1.5 kb (in both trl and irl) to 9 kb in irl ... | 1985 | 2989417 |
| synthesis and antiviral activity of 11-azapentacyclo[6.2.1.0.0.0]decane. | 11-azapentacyclo[6.2.1.0.0.0]decane (6a) as well as its 6,7-dimethyl derivative 6b was synthesized by a novel, four-step sequence that holds promise for the construction of a variety of cage compounds with bridging nitrogen atoms. the hydrochloride salt of 6a was shown to possess no antiviral activity against either the influenza virus a/victoria/3/75 or the herpes simplex viruses hsv-1 and hsv-2. | 1985 | 2989519 |
| synthesis and biological activities of 2-pyrimidinone nucleosides. 2. 5-halo-2-pyrimidinone 2'-deoxyribonucleosides. | 1-(2-deoxy-beta-d-ribofuranosyl)-5-bromo-2-pyrimidinone (brpdr) and 1-(2-deoxy-beta-d-ribofuranosyl)-5-iodo-2-pyrimidinone (ipdr) have been synthesized by condensation of the appropriate silylated bases 2a and 2b, respectively, with 3,5-bis-o-(p-chlorobenzoyl)-2-deoxy-alpha-d-ribofuranosyl chloride (8) in 1,2-dichloroethane, in the presence of sncl4, followed by separation of the anomeric blocked nucleosides via column chromatography and subsequent deprotection with methanolic ammonia. both brpd ... | 1985 | 2989522 |
| synthesis and antiherpetic activity of (s)-, (r)-, and (+/-)-9-[(2,3-dihydroxy-1-propoxy)methyl]guanine, linear isomers of 2'-nor-2'-deoxyguanosine. | racemic 9-[(2,3-dihydroxy-1-propoxy)methyl]guanine [(+/-)-indg], a new analogue of acyclovir (acv) and a structural analogue of 2'-nor-2'-deoxyguanosine (2'ndg), was synthesized and found to inhibit the replication of herpes simplex virus types 1 (hsv-1) and 2 (hsv-2). subsequently, its optical isomers, (r)- and (s)-indg, were prepared from chiral intermediates. the chloromethyl ethers of 1,2-di-o-benzyl-d- and -l-glycerol were made and reacted with tris(trimethylsilyl)guanine to give the 9-alky ... | 1985 | 2989523 |
| analysis of world-wide age-specific data for the association of cervical cancer with infections by herpes simplex virus type 2. | world-wide incidence data obtained from the scientific literature are used to expand to age-specific incidence rates the observation that the incidence of invasive cervical cancer in many human populations is related to the incidence of infections with herpes simplex virus type 2 (hsv-2) in the same community. the data for the incidence of the tumor were obtained from international registries. data for prevalence of hsv-2 antibodies were used to evaluate the incidence of past primary infections ... | 1985 | 2989664 |
| incidences of antibodies to hepatitis b, herpes simplex and cytomegalovirus in prostitutes. | the 170 prostitutes registered by the local public health authority (165 female, 5 male; ages 17-61) were serologically investigated by elisa and cft for the presence of hepatitis b virus (hbv) markers (n = 170), anti-cmv (n = 118) and anti-hsv 2 antibodies (n = 117). 4.7% (1.2%) were found to be hbs- (hbe-)antigen carriers; 1.2% revealed an acute hepatitis b (seroconversion of anti-hbc or igm-anti-hbc highly positive); 17.1% presented a past and resolved hepatitis b (anti-hbs positive). these r ... | 1985 | 2990124 |
| antiherpes effects and pharmacokinetic properties of 9-(4-hydroxybutyl) guanine and the (r) and (s) enantiomers of 9-(3,4-dihydroxybutyl)guanine. | three acyclic guanosine analogs with similar structures, the (r) and (s) forms of 9-(3,4-dihydroxybutyl)guanine and 9-(4-hydroxybutyl)guanine, were compared for antiherpes activity in vivo and in vitro. the three guanosine analogs were viral thymidine kinase-dependent inhibitors of virus multiplication. in cell cultures, (s)-9-(3,4-dihydroxybutyl)guanine was the least active of these three drugs against a variety of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) strains. this was also th ... | 1985 | 2990325 |
| analysis of the hsv-2 early ag-4 antigen. | genital herpes simplex virus type 2 (hsv-2) infections can be distinguished from present or past hsv-1 infections by an ag-4 antigen complement fixation assay. the assay which utilizes a 4 hour hsv-2 infected cell extract prepared at a multiplicity of infection (moi) of 1.0 pfu/cell, appears to consist of several viral proteins. studies using monoclonal antibodies, polyclonal rabbit hyperimmune serum, hsv-1 x hsv-2 intertypic recombinant viruses and polyacrylamide gel electrophoresis suggest tha ... | 1985 | 2990385 |
| crossed immunoelectrophoretic analysis of herpes simplex virus type 2 proteins. characterization of antigen-5. | herpes simplex virus type 2 proteins extracted from infected cells and analysed by crossed immunoelectrophoresis identified a nonglycosylated antigen named ag-5. the antigen contained two proteins when extracted from the agarose gel and the molecular weights were 128k and 91k. both proteins are located in the nucleus of the infected cells and the 128k is identical to icp-8. the 91k protein is based on the reactivity with monoclonal antibodies most likely the alkaline exonuclease mapped by presto ... | 1985 | 2990392 |
| retrieval of latent herpes simplex virus type 1 genetic information from murine trigeminal ganglia by superinfection with heterotypic virus in vivo. | mice previously latently infected with the f strain of herpes simplex virus type 1 (hsv-1) can be successfully colonized with a second virus strain if hsv-2 is introduced at the same peripheral site as hsv-1. on the other hand, hsv-1 strains seemed able mutually to exclude establishment of latency with each other. mice (3 months or 3 years after nasal infection) latently infected with hsv-1 were thus superinfected with hsv-2. the mice were sacrificed 2 days post-infection when hsv-2 replication ... | 1985 | 2991439 |
| the effect of triterpenoid compounds on uninfected and herpes simplex virus-infected cells in culture. i. effect on cell growth, virus particles and virus replication. | the related triterpenoid compounds carbenoxolone sodium (cbx) and cicloxolone sodium (ccx) have been investigated in clinical trials for treatment of herpes simplex virus (hsv) infections. when the drugs were tested in vitro, two dose-related effects on bhk cells became apparent: the rate of cell growth was reduced and the drugs exhibited cytotoxicity at high concentrations. flow 2002 cells, in contrast, were apparently unaffected by all drug concentrations tested. the effect of up to 3 days inc ... | 1985 | 2991440 |
| thymidine kinase-deficient herpes simplex virus type 2 genital infection in guinea pigs. | in guinea pigs, thymidine kinase-producing strains of herpes simplex virus type 2 replicated to high titer in the vagina and spinal cord, and animals developed severe clinical disease. infection with thymidine kinase-deficient virus resulted in similar vaginal virus titers; however, animals exhibited little or no clinical illness and only low titers of virus were detected in spinal cord homogenate cultures. neural and extraneural latent infection as well as recurrent infection were noted in anim ... | 1985 | 2991558 |
| drug-resistant mutants of herpes simplex virus type 2 with a mutator or antimutator phenotype. | 1985 | 2991710 | |
| enhanced efficacy of the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine in combination with gamma interferon against herpes simplex virus type 2 in mice. | the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine (dhpg) and recombinant mouse interferon gamma (rmuifn-gamma) were evaluated for their efficacy alone and in combination against a herpes simplex virus type 2 systemic infection in mice. intraperitoneally infected animals were treated once a day with the drugs at various concentrations for 5 days starting 24 h after inoculation. dhpg was given subcutaneously and rmuifn-gamma intraperitoneally. for dhpg, the effective dose at which 50 ... | 1985 | 2992368 |
| metabolism of the carbocyclic analogue of (e)-5-(2-iodovinyl)-2'-deoxyuridine in herpes simplex virus-infected cells. incorporation of c-ivdu into dna. | the carbocyclic analogues of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) and (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu), in which the sugar moiety is replaced by a cyclopentane ring and which have been designated as c-bvdu and c-ivdu, respectively, are, like their parent compounds bvdu and ivdu, potent and selective inhibitors of herpes simplex virus type 1 (hsv-1) and, to a lesser extent, herpes simplex virus type 2 (hsv-2) replication. we have now synthesized the radiolabeled c-ivdu analogue, ... | 1985 | 2993282 |
| the therapeutic efficacy of a xanthate compound on herpes simplex virus in skin lesions of mice and guinea-pigs. | xanthates have recently been shown to inhibit the replication of both dna and rna viruses in vitro. the antiviral activity was exerted only under acidic ph conditions. curative effects in vivo on herpes simplex virus (hsv)-induced skin lesions were only observed when the xanthate compound was administered in the form of an ointment containing acidic buffer (sodium phosphate ph 5.0). advanced hsv-2-induced skin lesions in mice were healed by topical treatment with the xanthate compound. hsv-1-ind ... | 1985 | 2993486 |
| identification of cross-reacting glycoproteins of four herpesviruses by western blotting. | monospecific rabbit antisera against purified herpes simplex virus type 1 (hsv-1) gb, gc or gd antigens or polyvalent rabbit antiserum against equine herpesvirus type 1 (ehv-1)-infected cells was used in western blotting to identify antigenically related proteins in cells infected with hsv-1, hsv-2, bovine mammillitis virus or ehv-1. monomeric and oligomeric polypeptides related to hsv-1 gb were found in cells infected with each of the four herpesviruses. the gc antiserum was specific for hsv-1 ... | 1985 | 2993488 |
| serum antibodies to the major hsv-2-specified dna-binding protein in patients with an acute hsv infection or cervical neoplasia. | the major hsv-2-specified dna-binding protein (icsp 11/12) was purified from hsv-2-infected cells. elisa and immunoblotting techniques were used to study its antigenicity in hsv-infected patients and patients with cervical neoplasia and control women. patients with an acute hsv-2 infection had clearcut antibody responses to the purified icsp 11/12 preparation. determination of the icsp 11/12 antibodies by elisa revealed considerably higher serum antibody levels in patients with cervical carcinom ... | 1985 | 2993495 |
| lack of oral hsv-2 in a college student population. | a population of individuals with a high incidence of genital herpes simplex virus type 1 (hsv-1), due most likely to oro-genital contact, was examined to determine the incidence of oral herpes simplex virus type 2 (hsv-2) infection. herpes simplex virus was isolated from the oral cavity of 43 college students whose symptoms ranged from singular lesions of the lips with minimal discomfort to severe oral disease with systemic involvement resulting in lymphadenopathy, chills, sweat, myalgia, and fe ... | 1985 | 2993498 |
| synthesis and antiherpes simplex virus activity of 9-[(1,3-dihydroxy-2-propylthio)methyl]guanine. | the synthesis of the thio analogue (thio-dhpg, 2) of 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine (dhpg, 1) is described. the synthesis of 2 proceeded via the condensation of acetoxymethyl sulfide 9 with diacetylguanine 10 to give the protected nucleoside analogue 11. although catalytic hydrogenolysis failed, the benzyl ether functionalities of 11 were successfully cleaved by an acetolysis reaction to furnish 14. ammonolysis of 14 gave 2, which was also transformed to sulfoxide 15 and sulfone 16. ... | 1985 | 2993615 |
| application of antibody to synthetic peptides for characterization of the intact and truncated alpha 22 protein specified by herpes simplex virus 1 and the r325 alpha 22- deletion mutant. | the alpha 22 protein is one of five proteins synthesized immediately after infection of permissive cells with herpes simplex virus 1 and 2 (hsv-1 and hsv-2). on the basis of the reported nucleotide sequence of the hsv-1 gene, we synthesized two peptides containing the predicted amino acids 12 through 23 (12 residues) and 21 through 36 (16 residues) in two hydrophilic domains near the n terminus of the protein. rabbit antisera made against these peptides were then used to characterize the alpha 2 ... | 1985 | 2993651 |
| virion component of herpes simplex virus type 1 kos interferes with early shutoff of host protein synthesis induced by herpes simplex virus type 2 186. | herpes simplex virus (hsv) strains hsv type 1 (hsv-1) kos and hsv-2 186 are representative of delayed and early shutoff strains, respectively, with regard to their ability to inhibit protein synthesis in friend erythroleukemia cells. when these cells were simultaneously infected with hsv-1 kos and hsv-2 186, hsv-1 kos interfered with the rapid suppression of globin synthesis induced by hsv-2 186. the observed interference was competitive and not due to exclusion of hsv-2 by hsv-1 at the level of ... | 1985 | 2993660 |
| cytomegalovirus and herpes simplex virus ascending myelitis in a patient with acquired immune deficiency syndrome. | progressive ascending myelitis was the presenting feature of the acquired immune deficiency syndrome (aids) in a homosexual man who also had kaposi's sarcoma, pneumocystis pneumonia, and disseminated cytomegalovirus (cmv) infection. neuropathological studies showed profuse cytomegalic cells throughout the brain and spinal cord, but no inflammatory response. at postmortem examination, cmv and herpes simplex virus, type 2 (hsv-2), were recovered from multiple sites throughout the central nervous s ... | 1985 | 2994553 |
| immunoblotting and enzyme-linked immunosorbent assay analysis of serological responses in patients infected with herpes simplex virus types 1 and 2. | serological responses to soluble membrane and cytoplasmic antigens specified by herpes simplex type 1 (hsv1) and 2 (hsv2) were studied by immunoblotting and by enzyme-linked immunosorbent assay (elisa). in the immunoblotting test, polypeptides migrating like the hsv1-specified glycoprotein c showed type-specific reactivity but could not always be detected. the immunoblotting and elisa results were in agreement when antibody responses to hsv1- and hsv2-specified antigens were compared, and they a ... | 1985 | 2995271 |
| differences in humoral immunogenicity between herpes simplex virus types 1 and 2. | infection of nmri mice with increasing doses of six different strains of herpes simplex virus type 1 (hsv-1) induced increasing levels of neutralizing antibodies. in contrast, three strains of hsv-2, irrespective of the dose, induced only marginal antibody responses. only strain hg 52 (hsv-2) at high doses of infection stimulated antibody formation. the virus content of some organs in 6- to 8-week-old mice appeared to be lower after hsv-2 than after hsv-1 infection. application of immune-modulat ... | 1985 | 2995556 |
| inhibition of herpes simplex virus replication in vitro by human cytotoxic t cell clones and natural killer cell clones. | the abilities of human cytotoxic t cell (ctl) clones and natural killer (nk) cell clones to inhibit the replication of herpes simplex virus (hsv) in vitro were shown. the specificities of clones inhibiting hsv replication were the same as those of cytotoxicity in hsv type specificity and hla restriction, i.e. hsv type 1 (hsv-1) and hsv type 2 (hsv-2) common ctl clones inhibited the replication of both hsv-1 and hsv-2 in autologous cells, but not in allogeneic cells. hsv-1-specific ctl clones inh ... | 1985 | 2995557 |
| acute and latent herpes simplex virus neurological disease in mice immunized with purified virus-specific glycoproteins gb or gd. | groups of 5-week-old balb/c mice were immunized intraperitoneally with approximately 10 micrograms of purified alum-precipitated glycoprotein gb or gd of either herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) origin. control mice received injections of alum-precipitated 1% bovine serum albumin (bsa). following a second immunization 4 weeks later, seroconversion was confirmed by demonstrating the presence of glycoprotein-specific antibody by immune precipitation. all animals were challenged ... | 1985 | 2995573 |