Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| fluorescence correlation spectrometry of the interaction kinetics of tetramethylrhodamin alpha-bungarotoxin with torpedo californica acetylcholine receptor. | fluorescence correlation spectroscopy (fcs) is suited to determine low concentrations (10(-8) m) of slowly interacting molecules with different translational diffusion coefficients on the level of single molecule counting. this new technique was applied to characterize the interaction dynamics of tetramethylrhodamin labelled alpha-bungarotoxin (b( *)) with the detergent solubilized nicotinic acetylcholine receptor (achr) of torpedo californica electric organ. at pseudo-first-order conditions for ... | 1996 | 17023344 |
| the ecological characteristics of anopheles mosquitoes associated with low malaria transmission in siwa oasis, egypt. | the ecology of anopheles sergentii and an. multicolor was investigated over eight months to examine the entomological factors maintaining the low plasmodium vivax transmission in siwa oasis. the two species were encountered as larvae while the dominant species, anopheles sergentii was also collected as adults. breeding sites were characterized for the two species in six localities. larvae of both species were common during june and november. in bahi el din as an indicator village with a higher e ... | 1996 | 17217007 |
| sexual behavior of donkey jacks: influence of ejaculatory frequency and season. | the courtship of 5 jacks was evaluated weekly for 12 mo. the characteristics of sexual behavior were recorded before the collection of 2 ejaculates, at 4-h intervals, into an artificial vagina in the presence of a female in either natural or induced estrus. the maximum time given to the jack to perform the ejaculatory mount was 1 h. if the jacks did not ejaculate, another attempt was made the following week. at the time of collection, the male and female remained free in a paddock (20 m2). the d ... | 1996 | 16727925 |
| determination of cadmium and copper with et-aas after electrochemical deposition on a graphite electrode. | the electrodeposition of cadmium and copper on a special graphite disk electrode has been performed at controlled potential. the electrode with the deposit has been inserted into the graphite atomizer hga-400 by an adapted automatic sampler for the final determination by et-ass. the sensitivity of determination has been 0.371 (microg l(-1))(-1) for cadmium and 0.025 (microg l(-1))(-1) for copper for 2 min electrodeposition and increased linearly with the time of deposition. the limit of detectio ... | 1996 | 15045399 |
| detection of leakage in inflatable genitourinary devices. | improved manufacturing techniques and stronger materials have significantly reduced but not eliminated the incidence of hydraulic leakage in the inflatable genitourinary devices. this study was designed to test the efficacy of pressure dissipation and volume loss as potential tests for the detection of site-specific hydraulic leakage in the inflatable penile prosthesis (ipp) and the artificial sphincter (as) and compare their efficacy with the current standard--the ohmmeter technique. | 1996 | 8560671 |
| glutamine increases argininosuccinate synthetase mrna levels in rat hepatocytes. the involvement of cell swelling. | glutamine, one of the most efficient substrates in the urea cycle, was found to induce the accumulation of argininosuccinate synthetase (ass) mrna in primary cultured rat hepatocytes. the inducing action of glutamine was obtained at various stages of development and a half-maximal effect was observed at about 3 mm glutamine. this effect was apparent from 6 h and persisted for at least 48 h. nh4cl addition up to 5 mm did not significantly change the ass mrna level. like glutamine, hypoosmotic med ... | 1996 | 8617286 |
| inhibition of borrelia burgdorferi-bound fibrinolytic enzymes by alpha2-antiplasmin, pai-1 and pai-2. | lyme disease is caused by borrelia burgdorferi. human plasminogen and urokinase-type plasminogen activator bind to the surface of the spirochete where plasmin is generated. we have suggested that bound urokinase and plasminogen are utilized by the organism to disseminate. we tested whether the physiological inhibitors of urokinase, plasminogen activator inhibitor-1 and -2 (pai-1, pai-2), could regulate the activity of spirochete-bound urokinase. the k(ass) of pai-1 and pai-2 for bound urokinase ... | 1996 | 8645243 |
| inhibition of neutrophil elastase by the alpha1-proteinase inhibitor-immunoglobulin a complex. | neutrophil elastase is thought to be involved in cartilage destruction occurring in rheumatoid arthritis despite the local presence of alpha1-proteinase inhibitor. part of synovial fluid alpha1-proteinase inhibitor forms a mixed disulfide with immunoglobulin a, which has been postulated to lack inhibitory activity. we show here that the immunoglobulin-inhibitor complex tightly inhibits neutrophil elastase and cathepsin g, bovine pancreatic trypsin and chymotrypsin, and porcine pancreatic elastas ... | 1996 | 8647251 |
| if you assume, you can make an ass out of u and me': a decade of the disector for stereological counting of particles in 3d space. | the year 1984 was a watershed in stereology. it saw the introduction of highly efficient and unbiased design-based methods for counting the number of arbitrary objects in 3-dimensional (3d) space using 2d sectional images. the only requirement is that the objects be unambiguously identifiable on parallel sections or successive focal planes. the move away from the ¿assumption-based' and ¿model-based' methods applied previously has been a major scientific advance. it has led to the resolution of s ... | 1996 | 8655396 |
| simulation studies of african horse sickness and culicoides imicola (diptera:ceratopogonidae). | a simulation model of african horse sickness in spain was developed to investigate what factors affect the likelihood of an epidemic after the introduction of the virus. the model included 2 host species (horses and donkeys) and 1 vector species (culicoides imicola kieffer). latin hypercube sampling was used for sensitivity analysis of the model, to include uncertainty in parameter estimates. in general, if an epidemic occurred most hosts were infected. the peak prevalence in midges was low, and ... | 1996 | 8667377 |
| cloning and analysis of the cdna for the common alpha-subunit of the donkey pituitary glycoprotein hormones. | reverse transcription-pcr was used to clone the coding region of the donkey (equus asinus) glycoprotein hormone alpha-subunit transcript from pituitary gland rna. the donkey alpha-subunit sequence demonstrated considerable identity with the horse (97% at the nucleotide level), confirming the very close evolutionary linkage between these two species. the predicted amino acid sequence revealed that the donkey alpha-subunit has the same unusual c-terminus as the horse alpha-subunit, when compared w ... | 1996 | 8672238 |
| [distribution of borna disease virus in naturally infected animals with clinical disease]. | borna disease (bd) is a naturally occurring enzootic encephalomyelitis of horses and sheep. the aetiological agent, borna disease virus (bdv) is an unclassified, neurotropic, negative stranded rna virus. the study aimed at providing further information on bd of naturally infected animals. samples obtained from 20 animals (18 horses, 1 donkey, 1 sheep) were investigated by a series of virological and molecular biological tests. the highly sensitive reverse transcription-polymerase chain reaction ... | 1996 | 8694747 |
| remotely delivered immunocontraception in free-roaming feral burros (equus asinus). | regulation of local overpopulations of free-roaming feral equids is in demand worldwide for ecological balance and habitat preservation. contraceptive vaccines have proven effective in feral horses, which breed seasonally, but no data are available for equids such as the burro, which is reproductively active all year round. in the present study, 27 individually identified female feral burros (equus asinus) roaming free in virgin islands national park (st john, us virgin islands; lesser antilles) ... | 1996 | 8699431 |
| enzymatic properties and inhibition by single-stranded autonomously replicating sequences of adenylosuccinate synthase from saccharomyces cerevisiae. | adenylosuccinate synthase (ass) from saccharomyces cerevisiae has been shown to bind specifically to the t-rich side of the autonomously replicating sequence (ars) core consensus sequence [zeidler, r., hobert, o., johannes, l., faulhammer, h. & krauss, g. (1993) j. biol. chem. 268, 20191-20197]. we have cloned and sequenced the gene for ass and have studied in detail the enzymatic properties and dna-binding activity of ass. the deduced amino acid sequence of the yeast ass is highly similar to th ... | 1996 | 8706758 |
| [identification and diagnosis of taylorella equigenitalis by a dna amplification method (pcr)]. | a polymerase chain reaction (pcr) for identification of taylorella equigenitalis was developed. the oligonucleotide primers are based on the dna sequence of the rrs gene of t. equigenitalis, encoding for the 16s ribosomal rna. analysis of 21 strains of t. equigenitalis from england, usa and switzerland showed an amplification product of 410 bp with identical sau3a restriction profile. the sensitivity of the pcr-assay was estimated to detect 50 to 500 bacteria of t. equigenitalis in a mixture wit ... | 1996 | 8721184 |
| ["arbitrarily primed pcr". or: chance in the service of science]. | "arbitrarily primed pcr" (ap-pcr) methods are based on the amplification of dna with arbitrarily selected primers. in the present review article two selected applications of this methodical approach are summarized. using the rapd ("random amplified polymorphic dna") technique the genetic variability of different organisms can be analyzed by generating a genomic fingerprint. echinococcus granulosus isolates (metacestodes) from spain and switzerland were comparatively characterized by the use of t ... | 1996 | 8721188 |
| nucleotide sequence of exons 5 to 9 of the p53 tumour-suppressor gene of the horse (equus caballus). | an evolutionary conserved 1.3 kb fragment corresponding to the horse p53 tumour suppressor gene was pcr amplified, cloned and the nucleotide sequence determined. the p53 fragment encoded exons 5 to 9 and the intervening introns. the nucleotide sequence and the predicted aminoacid sequence showed a high level of homology with human and donkey p53 sequences. | 1996 | 8722575 |
| pathogenetic mechanism and prevalence of the stable atlantoaxial subluxation in rheumatoid arthritis. | to study the pathogenetic mechanisms responsible for and prevalence of stable atlantoaxial subluxation (aas). | 1996 | 8724293 |
| pharmacokinetics and pharmacodynamics of phenylbutazone and oxyphenbutazone in the donkey. | 1996 | 8735424 | |
| failure of endometrial cup development in the donkey-in-horse model of equine abortion. | the mature preinvasive chorionic girdles of horse, mule, donkey and extraspecies donkey-in-horse conceptuses, and the very young endometrial cups on d 37 of gestation in mares carrying horse, mule and transferred donkey-in-horse conceptuses, were compared histologically and ultrastructurally to determine possible mechanisms underlying failure of endometrial cup development in the donkey-in-horse model of equine abortion. the progenitor chorionic girdle from the failing donkey-in-house pregnancy ... | 1996 | 8763475 |
| [paramphostomid infestation in equids in egypt]. | the investigation of 156 donkeys in the area of the veterinary faculty of the tanta university in kafr el-sheikh showed that 49 animals (31.41%) were infected with trematodes, 34 donkeys (21.80%) served as hosts for gastrodiscus aegyptiacus, 22 (14.10%) were infected with fasciola gigantica und 7 (4.49%) with both trematodes. 6 of 40 examined horses (15.0%) showed monoinfections with g. aegyptiacus only. because of the fragmentary knowledge on these paramphistomides of equids, some aspects conce ... | 1996 | 8765538 |
| [dysmenorrhea]. | primary dysmenorrhea is very frequent in ovulating women; it can even become a disease. in some cases, but not always, it is preceded by premenstrual tensions. secondary dysmenorrhoea should be clearly differentiated, because it is a symptom of uterine abnormalities or adnexal diseases, whereas primary dysmenorrhoea is an entity in itself. the most frequent cause of secondary dysmenorrhoea is endometriosis. dysmenorrhea membranacea is a rare event, but should not be forgotten. primary dysmenorrh ... | 1996 | 8767549 |
| endothelial cell dysfunction secondary to the adhesion of diabetic erythrocytes. modulation by iloprost. | we previously showed the correlation between the extent of vascular complications and erythrocyte adherence to endothelium in diabetes mellitus. the accumulation of advanced glycation end products (ages) on the erythrocyte surface in diabetes mediates their interaction with endothelial cells through a specific endothelial receptor for ages (rage). binding of diabetic erythrocytes to endothelial cells resulted in evidence of oxidant stress responsible for a range of cellular perturbations. in the ... | 1996 | 8971575 |
| kinetics of binding of antp homeodomain to dna analyzed by measurements of surface plasmon resonance. | the kinetics of binding of the antp homeodomain to three kinds of dna fragment were analyzed by measurements of surface plasmon resonance at various temperatures. non-specific and specific binding of the homeodomain to dna was examined. in the case of non-specific binding, the association rate constant (k(ass)) was estimated to be 1.41-2.62 x 10(5) m(-1) s(-1) and the dissociation rate constant (k(diss)) was 1.36-3.10 x 10(-2) s(-1), thus, the dissociation constant (kd) was 0.847-1.72 x 10(-7) m ... | 1996 | 8977123 |
| applications of pig zona pellucida immunocontraception to wildlife fertility control. | a unique application of pig zona pellucida (pzp) immunocontraception is the control of wildlife populations. a native pzp vaccine has been successfully applied to wild horse and donkey populations. a single annual booster inoculation was capable of maintaining contraception. seven consecutive years of pzp treatment in wild mares resulted in no detectable debilitating side effects, and reversibility of contraception has been documented among mares treated for up to 4 consecutive years. long-term ... | 1996 | 8984182 |
| long-chain fatty acids suppress the induction of urea cycle enzyme genes by glucocorticoid action. | in order to test the possibility that free fatty acids are the mediator of the abnormal expression of urea cycle enzyme genes in carnitine-deficient juvenile visceral steatosis (jvs) mice, the effects of fatty acids on urea cycle enzyme, carbamoylphosphate synthetase (cps) and argininosuccinate synthetase (ass), mrna levels were examined in rat primary cultured hepatocytes. addition of a synthetic glucocorticoid hormone, dexamethasone, caused increases in cps and ass mrnas. further addition of o ... | 1996 | 8985169 |
| observations on the prevalence of trypanosomosis in small ruminants, equines and cattle, in relation to tsetse challenge, in the gambia. | the prevalence of trypanosome infections in djallonké sheep and west african dwarf goats at different sites in the gambia showed a significant, positive correlation with contemporary assessments of tsetse challenge. a similar correlation was observed in village n'dama cattle which showed comparable prevalence values in the same areas. trypanosome prevalences also tended to be higher in horses and donkeys in areas with high tsetse challenge compared with sites with relatively few flies. a ranking ... | 1996 | 8988551 |
| [alpha 2-adrenoreceptor agonists--horse or donkey?]. | 1996 | 8991465 | |
| [endoparasites of donkeys and horses kept in communal housing in upper bavaria; species spectrum and incidence]. | in this epidemiological study of endoparasites 37 donkeys and 23 horses were included, which were examined coproscopically in regular intervals over a 15 month period. the animals derived from ten farms, in which regular prophylactic treatments with anthelmintics had been practiced at least for two years before this investigation. this study revealed the presence of at least twelve parasite species in donkeys, eimeria leuckarti, gasterophilus intestinalis, fasciola hepatica, anoplocephala perfol ... | 1996 | 8999593 |
| nosology of fetal hypokinesia sequence based on cns abnormalities: is there an aase-smith syndrome? | aase-smith syndrome (ass) is usually defined as a dominantly inherited combination of arthrogryposis, dandy-walker malformation and cleft palate. we describe a sporadic case of foetal akinesia with abnormal fossa posterior, fitting the diagnosis of ass, and discuss the nosology of this entity among syndromes with distal arthrogryposis. ass shows a "hybrid" phenotype: adults with mild ass could be classified as distal arthrogryposis, whereas severely affected newborns overlap with the marden-walk ... | 1996 | 9001811 |
| [jenner's cowpox vaccine in light of current vaccinology]. | two hundred years ago edward jenner inoculated james phipps with vaccinia and 181 years later smallpox had disappeared from the surface of the earth as a result of generalized vaccination. compared to the requirements of modern vaccinology, the procedures used by jenner and his successors, were extremely primitive because of an almost total lack of knowledge in the field of microbiology and immunology. the active principle of smallpox vaccine is vaccinia virus, which in many respects, differs fr ... | 1996 | 9027132 |
| improvement of the tissue integration of a new modified polytetrafluoroethylene prosthesis: mycro mesh. | we studied the behaviour of the different tissue interfaces formed on a new type of prosthesis used for the repair of abdominal wall defects, mycro mesh (w. l. gore and ass., flagstaff, az, usa), which consists of perforated layers of polytetrafluoroethylene (ptfe). in 20 new zealand white rabbits, a full-thickness (except skin) 7 cm x 5 cm defect was created in the anterior abdominal wall. the defects were repaired with a prosthetic implant (mycro mesh) that was placed in direct contact with ab ... | 1996 | 8805973 |
| hypertrophic osteopathy (marie's disease) in equidae: a review of twenty-four cases. | the case records of 23 horses and one donkey affected by hypertrophic osteopathy (ho) (marie's disease) were reviewed. all affected animals presented with limb swellings, which were bilaterally symmetrical and usually involved both fore- and hindlimbs. associated signs included stiffness/lameness and weight loss. radiological features included periosteal new bone formation over the diaphyses and metaphyses of affected bones. the metacarpal and metatarsal bones were most frequently affected. arti ... | 1996 | 8818590 |
| inhibition of coagulation factors by recombinant barley serpin bszx. | barley serpin bszx is a potent inhibitor of trypsin and chymotrypsin at overlapping reactive sites (dahl, s.w., rasmussen, s.k. and hejgaard, j. (1996) j. biol. chem., in press). we have now investigated the interactions of bszx with a range of serine proteinases from human plasma, pancreas and leukocytes, a fungal trypsin and three subtilisins. thrombin, plasma kallikrein, factor viia/tissue factor and factor xa were inhibited by bszx at heparin independent association rates (k(ass)) of 4.5 x 1 ... | 1996 | 8843156 |
| efficacy of triclabendazole against fasciola hepatica in the donkey (equus asinus). | 1996 | 8863404 | |
| the mitochondrial dna molecule of sumatran orangutan and a molecular proposal for two (bornean and sumatran) species of orangutan. | the complete mitochondrial dna (mtdna) molecule of sumatran orangutan, plus the complete mitochondrial control region of another sumatran specimen and the control regions and five protein-coding genes of two specimens of bornean orangutan were sequenced and compared with a previously reported complete mtdna of bornean orangutan. the two orangutans are presently separated at the subspecies level. comparison with five different species pairs-namely, harbor seal/grey seal, horse/donkey, fin whale/b ... | 1996 | 8875856 |
| the complete mitochondrial dna (mtdna) of the donkey and mtdna comparisons among four closely related mammalian species-pairs. | the nucleotide sequence of the complete mitochondrial genome of the donkey, equus asinus, was determined. the length of the molecule is 16,670 bp. the length, however, is not absolute due to pronounced heteroplasmy caused by variable numbers of two types of repetitive motifs in the control region. the sequence of the repeats is (a) 5'-cacaccca and (b) 5'-tgcgcgca, respectively. the order of (a) and (b) can be expressed as {n[2(a)+(b)]+m(a)}. in 32 different clones analyzed the number of n and m ... | 1996 | 8875857 |
| synteny mapping in river buffalo. | the cosegregation of ten coding loci has been investigated, in a panel of 37 somatic cell hybrids resulting from the fusion of a hamster cell line and river buffalo lymphocytes, by use of southern hybridization technique. five syntenic groups, tcrb-pgy3, ass-abl, fuca1p-cryg, mbp-yes1, and cgn1-acta1, previously assigned to cattle as u13, u16, u17, u28, and u29 respectively, were also found to be syntenic in buffalo. based on the extensive syntenic conservation and banding homology between cattl ... | 1996 | 8875891 |
| a sequence similarity search algorithm based on a probabilistic interpretation of an alignment scoring system. | we present a probabilistic interpretation of local sequence alignment methods where the alignment scoring system (ass) plays the role of a stochastic process defining a probability distribution over all sequence pairs. an explicit algorithms is given to compute the probability of two sequences given and ass. based on this definition, a modified version of the smith-waterman local similarity search algorithm has been devised, which assesses sequence relationships by log likelihood ratios. when te ... | 1996 | 8877503 |
| nationwide serological survey of equine influenza in nigeria. | the objective of this work was to examine the incidence of equine influenza viruses in the equine population of an area of tropical africa where equine influenza virus activity has recently been reported for the first time. a serological survey of sera from horses and donkeys from regions of nigeria taken from 1990 to 1993 was carried out and the results obtained were com-pared with equine sera from western europe (ireland). the sera were assayed for presence of antibodies by both haemagglutinat ... | 1996 | 8881415 |
| [prevalence of rhinoestrus usbekistanicus (gan 1947) in donkeys (equus asinus) in senegal]. | a survey performed in dakar (senegal) showed, for the first time, the presence of rhinoestrus usbekistanicus in donkeys in the south of the sahara desert. out of the 138 heads examined between november and june, 84% were infected by a mean burden of 15.9 larvae (9.7 l1, 1.8 l2 and 4.1 l3). there is a hypobiotic period starting in march during the dry season. | 1996 | 8881418 |
| a radioimmunoassay for the determination of tipredane in human plasma. | a sensitive method for the determination of tipredane in human plasma has been developed. prior to radioimmunoassay, proteins are removed from plasma by precipitation with acetonitrile. a [125i]iodohistamide derivative of tipredane is used as a heterogeneous radioligand. the primary antiserum is raised in sheep against a tipredane (o-carboxymethyl) oxime-bovine serum albumin conjugate. donkey anti-sheep igg antiserum is used as the secondary antiserum in a double antibody separation procedure. t ... | 1996 | 8895073 |
| the complete mitochondrial dna sequence of the greater indian rhinoceros, rhinoceros unicornis, and the phylogenetic relationship among carnivora, perissodactyla, and artiodactyla (+ cetacea). | the sequence (16,829 nt) of the complete mitochondrial genome of the greater indian rhinoceros, rhinoceros unicornis, was determined. like other perissodactyls studied (horse and donkey) the rhinoceros demonstrates length variation (heteroplasmy) associated with different numbers of repetitive motifs in the control region. the 16,829-nt variety of the molecule includes 36 identical control region motifs. the evolution of individual peptide-coding genes was examined by comparison with a distantly ... | 1996 | 8896369 |
| don't dissect use the disector! 'if you assume, you can make an ass out of u and me': a decade of the disector for sterological counting of particles in 3d space. | 1996 | 8906439 | |
| comparative morphology of the pectinate ligaments of domestic mammals, as observed under the dissecting microscope and the scanning electron microscope. | the pectinate ligaments of ten horses, two donkeys, five oxen, five sheep, ten goats, five dogs, five cats, thirty pigs and two rabbits were studied under the stereomicroscope and the scanning electron microscope. in the horse and the donkey, the pectinate ligament was very prominent and was characterized by sturdy interconnected strands and relatively small intertrabecular spaces. the pectinate ligaments of ruminants were composed of shorter strands, separated by relatively larger spaces. fusio ... | 1996 | 8915997 |
| transmission of the south african asinine strain of equine arteritis virus (eav) among horses and between donkeys and horses. | lateral and sexual transmission of eav among horses and lateral transmission between donkeys and horses were attempted by experimental infection with the south african asinine strain. clinical, immunological and virological responses were evaluated. all intramuscularly inoculated horses developed very mild clinical signs, were viraemic, shed virus from nasopharynx, and seroconverted. lateral infection was demonstrated in one in-contact mare. reinfection of two stallions by intranasal instillatio ... | 1996 | 8917855 |
| heterophile antibodies to chicken erythrocytes in sheep infected with trypanosoma congolense. | high hemagglutinin titres against chicken erythrocytes were detected in the sera of trypanosoma congolense-infected sheep. adsorption of sheep sera with solubilized t. congolense resulted in marked reduction of hemagglutinin titre. heat inactivation of the sera at 56 degrees c for 30 min had no demonstrable effects on the hemagglutinin titre. sera collected from the sheep before trypanosome infection did not agglutinate chicken erythrocytes. on the other hand, erythrocytes of horse, donkey and d ... | 1996 | 8917863 |
| analysis of the long terminal repeat from a cytopathic strain of equine infectious anemia virus. | sequential passage of the tissue culture-adapted prototype strain of eiav in fetal donkey dermal (fdd) cell cultures generated a virus stock which exhibits cytopathic effects in fdd cell cultures. in this study, the effects of the long terminal repeat (ltr) region on virus replication and cytopathogenicity were examined. the fdd-adapted virus ltr was found to contain a number of base pair mutations and a large insertion within the u3 region in comparison with the previously characterized ltr, la ... | 1996 | 8918926 |
| prevalence of sarcocysts in livestock of northwest ethiopia. | a survey of sarcocystis was conducted in cattle, sheep, goats, donkeys and chickens. a total of 671 haematoxylin-eosin (h-e) stained muscle tissue samples, including diaphragm, masseter, cardiac and oesophageal musculatures were examined. additionally, cardiac muscle samples from 40 fetuses were included. an infestation rate of 93% in sheep, 82% in cattle, 81% in goats, 16.6% in donkeys and 6.6% in chickens was noted. the infestation rate of diaphragm, masseter, cardiac and oesophageal musculatu ... | 1996 | 8919969 |
| pharmacokinetics of ketoprofen in the donkey (equus asinus). | the pharmacokinetic parameters of ketoprofen were determined in four donkeys after a single intravenous injection of a dose of 2.2 mg/kg body weight. the total body clearance (clb) was 414.0 +/- 98.70 ml/h/kg (mean +/- sd), the volume of distribution at steady state (vss) 263.10 +/- 55.43 ml/kg and the elimination half-life 1.30 +/- 0.75 h. these values were compared to those obtained in horses. | 1996 | 8921728 |
| alterations in glomerular anionic sites in the autologous phase of canine anti-glomerular basement membrane nephritis. | there have been a few studies on canine nephrotoxic glomerulonephritis produced by anti-glomerular basement membrane serum (agbm), but these reports have not focused on an alteration in the charge properties of glomerular basement membrane (gbm). in this study, rabbit agbm or normal rabbit serum (nrs) was given intravenously (2 ml/kg body weight) to 16 male beagle dogs. an alteration of anionic sites (ass) of gbm was studied quantitatively using polyethyleneimine as a cationic probe by electron ... | 1996 | 8923685 |
| [preparation and study of recombinant strains of venezuelan equine encephalomyelitis virus expressing the pres2-s gene of the hepatitis b virus]. | venezuelan equine encephalomyelitis (vee) virus-based vectors for expression of heterologous genes have been constructed using full-length cdna copy of the interstrain recombinant virus (trinidad donkey and 230 strain). gene cassettes carrying the subgenomic mrna promoter of vee virus and the pres2-s gene of hepatitis b virus (hbv) were inserted before or after the genes of structural viral proteins. live virus stocks were obtained by transfection of chick embryo fibroblasts with in vitro transc ... | 1996 | 8928499 |
| anisoosmotic regulation of hepatic gene expression. | the effect of anisoosmolarity on the abundance of various mrna species was examined in perfused rat liver and h4iie rat hepatoma cells. hyperosmotic exposure (385 mosmol/l) of isolated rat livers increased mrna levels for tyrosine aminotransferase (tat) by 246% and those for phosphoenolpyruvate carboxykinase (pepck) by 186%, whereas hypoosmotic exposure (225 mosmol/l) decreased their levels to 43% and 42%, respectively. mrna levels for fructose-1,6-bisphosphatase (fbp), argininosuccinate lyase ( ... | 1996 | 8929814 |
| smooth-pursuit eye movement and saccadic intrusions in obsessive-compulsive disorder. | although several reports agree that smooth-pursuit eye movement (spem) is abnormal in some obsessive-compulsive disordered (ocd) patients, differences between treatments and lack of accuracy in control selection make the results controversial. although reduced gain seems the most accepted abnormality, the characteristics of saccadic disruption of smooth pursuit are as yet unspecified. spems in 21 ocd patients (dsm-iii-r) and 21 healthy subjects recruited from the community were studied through a ... | 1996 | 8931920 |
| essential thrombocythemia and pregnancy. | a review of the literature disclosed 106 pregnancies (preg.) in 57 women with essential thrombocythemia (et). the success rate (baby alive) was 57% (60 live births/106 preg.), the rate of miscarriage 43% (46 miscarriages/106 preg.). the most frequent complication was spontaneous abortion during the first trimester in 36% (38 abortions/106 preg.). other complications such as intrauterine death and stillbirth after the 28th week, which occurred in 5% (7/106), premature delivery in 8% (8/106), pre- ... | 1996 | 8951773 |
| [immunopathology of borna disease in the horse: clinical, virological and neuropathologic findings]. | tissues from nine horses and one donkey suffering from natural borna disease were investigated. clinically, all animals demonstrated progressive reduced mentation and aggravating gait disturbances. during the clinical course anorexia and progressive loss of proprioception were observed. cranial nerve failure was accompanied by signs of pharyngeal paralysis, sialorrhea, bruxism, and by blindness. virologically, infectious virus was detected in the brain of all animals investigated but was not fou ... | 1996 | 9139422 |
| [superselective spinal anesthesia for saphenectomy intervention. comparison of 2 concentrations of hyperbaric bupivacaine]. | high concentrations of intrathecal local anaesthetics may be neurotoxic. the purpose of this study was to compare the features of superselective spinal anaesthesia (ass) obtained with an equivalent dose (5 mg) of 0.5% (available now also in italy) and 1% hyperbaric bupivacaine. | 1996 | 9102589 |
| [the research of radioimmunoassay using double abs for bradykinin]. | bradykinin (bk) is a potent vasodilative substance, and plays great physiological and pathological roles in animals and human beings. to measure the quantity of bk, the radioimmunoassay (ria) has been devised, but the traditional ria method has certain defects, such as presence of numerows interfering factors and errors and time consuming. now, we produce anti-bk serum in rabbits by using bk-ovalbumin conjugate as an immunogen, and the 125i labeled tyr8-bk by using a modified chloramine-t method ... | 1996 | 9388945 |
| preparation and structures of the 2.2.2-cryptand(1+) salts of the [sb(2)se(4)](2)(-), [as(2)s(4)](2)(-), [as(10)s(3)](2)(-), and [as(4)se(6)](2)(-) anions. | 2.2.2-cryptand(1+) salts of the [sb(2)se(4)](2)(-), [as(2)s(4)](2)(-), [as(10)s(3)](2)(-), and [as(4)se(6)](2)(-) anions have been synthesized from the reduction of binary chalcogenide compounds by k in nh(3)(l) in the presence of the alkali-metal-encapsulating ligand 2.2.2-cryptand (4,7,13,16,21,24-hexaoxa-1,10-diazabicyclo[8.8.8]hexacosane), followed by recrystallization from ch(3)cn. the [sb(2)se(4)](2)(-) anion, which has crystallographically imposed symmetry 2, consists of two discrete edge ... | 1996 | 11666829 |
| survival analysis approach to risk factors associated with hyperlipemia in donkeys. | to investigate the effect of gender, body condition, and age as risk factors for the development of hyperlipemia in a population of donkeys. | 1996 | 8870744 |
| pharmacokinetics of gentamicin in donkeys. | 1996 | 8735430 | |
| estimation of the liveweight and body condition of working donkeys in morocco. | the age, sex, liveweight and body measurements (heart girth, umbilical girth, height, length from elbow to tuber ischii and circumference of the foreleg cannon bone) of 516 donkeys used to transport goods in morocco were recorded. there were few donkeys over 12 years of age. for adult donkeys weighing from 74 to 252 kg, the best equation with only one variable for predicting liveweight was: liveweight (kg) = heart girth (cm)2.65/2188. the inclusion of two variables improved the prediction margin ... | 1996 | 8686139 |
| preliminary pharmacokinetic and pharmacodynamic studies on flunixin meglumine in donkeys. | 1996 | 8908727 | |
| pharmacokinetics and cardiopulmonary effects of guaifenesin in donkeys. | five donkeys and three horses were given guaifenesin, intravenously, by gravity administration, until recumbency was produced. the time and dose required to produce recumbency, recovery time to sternal and standing were recorded. blood samples were collected for guaifenesin assay at 10, 20, 30, 40, 50, 60 min, and 2, 3, 4 and 6 h after guaifenesin administration. serum was analysed for guaifenesin using hplc and pharmacokinetic values were calculated using a computer software package (rstrip). i ... | 1997 | 9430767 |
| overloading of donkeys. | 1997 | 9447682 | |
| comparative pharmacokinetics of caffeine and three metabolites in clinically normal horses and donkeys. | to determine whether clearance of capacity-limited drugs in horses differs from that in donkeys by comparing the serum disposition of caffeine and its metabolites, theophylline, theobromine, and paraxanthine after i.v. administration of caffeine to horses and donkeys. | 1997 | 9256975 |
| longitudinal and cross-sectional studies to evaluate the risk of sarcoid associated with castration. | in order to investigate whether gender and castration have an effect on the time to the development of sarcoids, a retrospective study in a population of donkeys was conducted using survival analysis techniques. univariable kaplan meier product limit curves identified males as having significantly lower survival probability, or higher risk of developing sarcoids, than females (p < 0.01). cox's proportional hazard model was used to assess the effect of age at entry to the population whilst simult ... | 1997 | 9114958 |
| head-to-head linked double calix[4]arenes: convenient synthesis and complexation properties. | combination of calix[4]arenes functionalized at the upper rim at the 5- and 17-positions with amino and formyl groups, respectively, gives a new series of "head-to-head" linked double calix[4]arenes in nearly quantitative yield. the x-ray structure of a modified double calix[4]arene is reported. the novel, highly preorganized receptor molecules complex silver(i) ions (k(ass) = 9.5 x 10(5) m(-)(1) in cdcl(3)); the selectivity of complexation was studied by supported liquid membrane transport expe ... | 1997 | 11671587 |
| [local therapy with capsaicin or ass in chronic pain]. | to provide a brief review of the current state of topical treatment with capsaicin or acetylsalicylic acid (asa) for therapy of chronic pain syndromes. | 1997 | 12799806 |
| regulation of argininosuccinate synthetase mrna level in rat foetal hepatocytes. | expression of the hepatic gene for argininosuccinate synthase (ass), one of the key enzymes of the urea cycle, was analysed during the perinatal period in the rat. to this end, the amount of specific mrna was measured in the liver at various stages of development and in cultured foetal hepatocytes maintained in different hormonal conditions. the ass mrna was first detected in 15.5-day foetuses and its level increased concomitantly with a rise in the enzyme activity, suggesting that the appearanc ... | 1997 | 9395312 |
| making an ass of the law. | 1997 | 9401388 | |
| evidence that the alpha-subunit influences the specificity of receptor binding of the equine gonadotrophins. | horse lh/chorionic gonadotrophin (elh/cg) exhibits, in addition to its normal lh activity, a high fsh activity in all other species tested. donkey lh/cg (dklh/cg) also exhibits fsh activity in other species, but about ten times less than the horse hormone. in order to understand the molecular basis of these dual gonadotrophic activities of elh/cg and dklh/cg better, we expressed, in cos-7 cells, hybrids between horse and donkey subunits, between horse or donkey alpha-subunit and human cg beta (h ... | 1997 | 9415058 |
| differential effects of somatostatin analogues on proliferation of murine colonic cancer cells in vitro. | the effects of somatostatin analogues octreotide (sms 201-995), ass-51 and ass-52 on [3h]-thymidine incorporation into dna of the murine colon 38 cancer cells in vitro were investigated. it was found that sms 201-995 and ass-51 inhibited the tritiated thymidine incorporation in a dose-dependent manner. in contrast, analogue ass-52 in spite of a very similar structure to ass-51, which differed from the latter only by one ch2oh group, was devoid of remarkable antiproliferative activity. these resu ... | 1997 | 9418016 |
| carbon dioxide laser as a surgical instrument for sarcoid therapy--a retrospective study on 60 cases. | the objective of this retrospective clinical study was to evaluate the carbon dioxide laser in the treatment of single and multiple sarcoids in 60 animals (44 horses, 13 donkeys, 2 mules, and 1 pony). only animals that had been operated on 6 mo or more ago were included. recurrence, new manifestation rate, and cosmetic outcome were determined. recurrence was observed in 23 (38%) individuals. animals with new sarcoid manifestation with or without recurrence of a sarcoid were observed in 35 cases ... | 1997 | 9426943 |
| genus-specific detection of alphaviruses by a semi-nested reverse transcription-polymerase chain reaction. | a reverse transcription-polymerase chain reaction (rt-pcr) was developed for the genus-specific detection of alphaviruses. based on the available published sequences, degenerate primers were designed to ensure hybridization to a conserved region within the nonstructural protein 1 gene of all alphavirus species. the expected 434-basepair (bp) cdna fragment was amplified from all 27 alphavirus species by using rna extracted from 200 microl of infected cell culture supernatant. in addition, eight s ... | 1997 | 9430533 |
| an epizootic of besnoitiosis in goats in fars province of iran. | over 100,000 goats were grazing freely in isolated herds of 100 to 600 goats in a mountainous area of more than 2000 km2 to the north-east of darab city in fars province in southern iran. twenty to fifty percent of the goats in this area were infected with besnoitiosis. sheep, cattle and donkeys grazing in the same area showed no clinical manifestations of the infection. the diagnosis of the infection in the clinically suspected animals was confirmed by histological studies on skin biopsies from ... | 1997 | 9444079 |
| [prospective study on the prevention of heterotopic ossification after total hip replacement. non-steroidal anti-inflammatory agents versus radiation therapy]. | two prospective trials were undertaken to assess the comparative efficacy of early postoperative irradiation with different radiation doses versus the postoperative use of nonsteroidal antiinflammatory drugs (nsaid) for prevention of heterotopic ossification (ho) following prothetic total hip replacement (thp). | 1997 | 9454352 |
| elucidation of the mechanism enhancing the avidity of lectin with oligosaccharides on the solid phase surface. | the mechanism underlying molecular recognition of lectins was elucidated by a novel solid phase binding assay system based on surface plasmon resonance. when the apparent affinities of interactions between chitooligosaccharides and wheat germ agglutinin were compared between lectin-immobilized and oligosaccharide-immobilized assay systems, the affinity constants (ka) calculated for the former system were in good agreement with the previously reported values measured in solution. on the other han ... | 1997 | 9455921 |
| distribution and co-localization of nitric oxide synthase and argininosuccinate synthetase in the cat hypothalamus. | argininosuccinate synthetase (ass) and nitric oxide synthase (nos) comprise part of the cyclic metabolic pathway to produce nitric oxide (no). ass is one of the arginine synthesis enzymes which synthesizes argininosuccinate from aspartate and citrulline, and nos forms no and citrulline from arginine. this study examines the localization of ass and nos in the cat hypothalamus using nicotinamide adenine dinucleotide phosphate-diaphorase (nadph-d) histochemistry and immunohistochemistry against ass ... | 1997 | 9477156 |
| [pathophysiology and therapeutic concepts in coronary restenosis]. | demonstration of a reduced restenosis rate after stent implantation (benestent, stress) has initiated rapid increase in stent implantation rates with widening indications. at present, the majority of stents are implanted in "none-benestent/stress-lesions" with the consequence of a higher restenosis rate as previously expected. stent restenosis has therefore become a relevant problem in interventional cardiology. in contrast to balloon angioplasty, where acute and subacute recoil represents the m ... | 1997 | 9483438 |
| screening for bovine papillomavirus in peripheral blood cells of donkeys with and without sarcoids. | papillomaviral dna has been identified in peripheral blood cells of both cattle and humans with and without associated disease and it has been suggested that such cells may act as sites of viral latency. in order to investigate the possibility of latent papillomaviral infection in the aetiopathogenesis of the equine sarcoid, peripheral blood derived dna samples from 20 healthy and 34 sarcoid-affected donkeys were subject to polymerase chain reaction (pcr) using papillomaviral specific primers. a ... | 1997 | 9491459 |
| babesiosis in china. | the importance of babesiosis in livestock in china is discussed and mainly focused on bovine and equine babesiosis. babesiosis is still one of the most important diseases affecting livestock and has caused great economic loss. nine species of babesia have been recognized in livestock: b. bigemina, b. bovis, b. major, b. motasi, b. ovis, b. perroncitoi, b. trautmanni, b. equi (theileria equi), b. caballi. the distribution of babesia follows the distribution of the tick vectors. the main vectors o ... | 1997 | 9512738 |
| wound infection caused by staphylococcus hyicus subspecies hyicus after a donkey bite. | staphylococcus hyicus subspecies hyicus has not previously been reported to cause human infections, but is a well known cause of diseases in a variety of animals. we report a wound infection following a donkey bite. | 1997 | 9112308 |
| apoptotic cell death is an important cause of neuronal injury in experimental venezuelan equine encephalitis virus infection of mice. | mice develop a fatal encephalomyelitis after infection with the trinidad donkey strain of venezuelan equine encephalitis (vee) virus. adult mice were inoculated intraperitoneally with vee virus and the brains were examined at different time points. morphological changes were assessed by histological staining. vee virus antigen was detected with immunoperoxidase staining, and dna fragmentation was evaluated in situ using the terminal deoxynucleotidyl transferase-mediated dutp-digoxigenin nick end ... | 1997 | 9113200 |
| clinical and molecular genetic characterisation of a family segregating autosomal dominant retinitis pigmentosa and sensorineural deafness. | to characterise clinically a large kindred segregating retinitis pigmentosa and sensorineural hearing impairment in an autosomal dominant pattern and perform genetic linkage studies in this family. extensive linkage analysis in this family had previously excluded the majority of loci shown to be involved in the aetiologies of rp, some other forms of inherited retinal degeneration, and inherited deafness. | 1997 | 9135384 |
| [solid-phase immunoenzyme ass for detecting giardia lamblia antigens]. | an elisa sandwich for the detection of giardia lamblia antigens in human faeces was standardized. 175 samples were studied: 77 positive, 61 negative to cysts and/or trophozoites by direct faeces test, and 19 positive to other parasite different from g. lamblia. the sensitivity of the technique was 94.8% and the specificity 98.3%. the method detects an antigen concentration of 31 ng. the procedure is simple, sensitive and specific so, it may be useful for diagnosis and in epidemiological studies. | 1997 | 9685962 |
| in vitro propagation of theileria annulata infected schizonts in different media supplemented with heterologous sera. | efficacy of medium rpmi-1640 (supplied by gibco usa, centron and hi-media) supplemented with horse, donkey, sheep and goat sera was evaluated for in vitro propagation of theileria annulata (hisar) infected bovine mononuclear cells. the results were compared with the growth rate in rpmi-1640 supplemented with foetal bovine serum (gibco). rpmi-1640 (gibco) proved to be the best medium for in vitro cultivation of the parasite infected cells. foetal bovine serum could be easily, safely and reliably ... | 1997 | 9567745 |
| [lead in the blood and protoporphyrin levels in erythrocytes in glass workers exposed to lead]. | this paper investigates how the indicators of lead exposure (blood lead-pbb, delta-aminolevulinic acid dehydratase-delta-alad, erythrocyte protoporphyrin-ep), relate to the exposure duration prior to the analyses, alcohol intake, gamma-glutamyltranspherase (ggt), and smoking habits of 25 glass workers exposed to lead oxide over a period between 1988 and 1994. the pbb was analyzed with the et ass method, (alad with standardized european method) while ep with the spectrofluorometric method. the al ... | 1997 | 9721457 |
| pancreatic secretory trypsin inhibitor as a diagnostic marker for adult-onset type ii citrullinemia. | adult-onset type ii citrullinemia is characterized by which deficiency of argininosuccinate synthetase (ass) protein is found specifically in the liver of patients. our recent study using differential messenger rna (mrna) display showed that the expression of human pancreatic secretory trypsin inhibitor (hpsti) mrna increases significantly in the liver of all type ii patients tested. in the present work, we found that the concentration of hpsti protein was higher in the liver of type ii patients ... | 1997 | 9141434 |
| fine localization of the torsion dystonia gene (dyt1) on human chromosome 9q34: yac map and linkage disequilibrium. | the dyt1 gene, which maps to chromosome 9q34, appears to be responsible for most cases of early-onset torsion dystonia in both ashkenazic jewish (aj) and non-jewish families. this disease is inherited in an autosomal dominant mode with reduced penetrance (30%-40%). the abnormal involuntary movements associated with this disease are believed to be caused by unbalanced neural transmission in the basal ganglia. previous linkage disequilibrium studies in the aj population placed the dyt1 gene in a 2 ... | 1997 | 9149944 |
| localization of regulatory peptides in the male urogenital apparatus of domestic equidae: a comparative immunohistochemical study in equus caballus and equus asinus. | an immunohistochemical study was carried out on specimens of testis, excurrent duct including the male accessory glands and urethra in its various tracts in the horse and the donkey, in order to localize nine regulatory peptides. immunoreactivities were tested by means of labelled strept avidin-biotin (lsab) method. the study has shown that equine male genitalia are supplied by many peptide immunoreactive nerves containing npy-, vip-, leu- and met-enkephalin-, substance p-, cgrp- and bombesin/gr ... | 1997 | 9151118 |
| differences in a ribosomal dna sequence of lungworm species (nematoda:dictyocaulidae) from fallow deer, cattle, sheep and donkeys. | the second internal transcribed spacer (its2)* of the ribosomal dna of dictyocaulus viviparus from cattle, d eckerti from fallow deer. d filaria from sheep and d arnfieldi from donkeys has been sequenced to investigate the genetic relationships among lungworm species, especially between d viviparus and d eckerti, because the latter is not generally accepted as a separate species. the length of the its2 varied between 403 and 481 bases, and its gc content ranged from 25 to 33 per cent. intraspeci ... | 1997 | 9160418 |
| simulation studies of vaccination strategies in african horse sickness. | a simulation model including two hosts (horses and donkeys) and one vector (culicoides imicola) for african horse sickness in spain is extended to consider vaccination strategies. if hosts were protected prior to virus introduction, elimination of simulated epidemics was related nonlinearly to the fraction protected. protecting donkeys as well as horses increased the effectiveness of vaccination. prevention of 50% of epidemics required 75% coverage of horses and donkeys or 90% coverage of horses ... | 1997 | 9160519 |
| glucocorticoids play an important role in mediating the enhanced metabolism of arginine and glutamine in enterocytes of postweaning pigs. | weaning is associated with increased intestinal metabolism of glutamine and arginine as well as elevated plasma concentrations of cortisol (the major circulating glucocorticoid) in pigs. the objective of this study was to determine if cortisol plays an important role in mediating the enhanced amino acid metabolism in enterocytes of weaned pigs by administering ru486 (a glucocorticoid receptor antagonist). eighteen 21-d-old pigs were randomly assigned to three groups of six. two of these groups r ... | 1997 | 9164994 |
| [secondary prevention of ischemic strokes: effect of dosage of aspirin]. | the value of acetylsalicylic acid (ass) in the secondary prevention of ischemic stroke is well established. however, the optimum dose of aas for stroke-threatened patients remains unsettled. this paper reviews the pattern of adverse reactions to aas and their relationship to the dosage of ass evaluated. | 1997 | 9172913 |
| the effect of ammonium chloride on hepatic and renal metabolism in the rat. | the metabolic effects of an ammonium salt on the liver and kidney were investigated. rats were allowed free access to a 0.28 m ammonium chloride (nh4cl) solution for 7- and 8-day periods. serum urea concentration was significantly increased after 8 days of nh4cl ingestion. however the following hepatic urea cycle enzymes remained unchanged: cps, otc, ass and asl. the pattern of urinary urea excretion was variable. when the data for the 7-day period were pooled, there was no significant differenc ... | 1997 | 9192912 |
| cloning, sequencing and in vitro functional expression of recombinant donkey follicle-stimulating hormone receptor: a new insight into the binding specificity of gonadotrophin receptors. | among all mammalian fsh receptors (fsh-r; including donkey (dk) fsh-r), only horse (hs) fsh-r does not bind hslh/chorionic gonadotrophin (cg). in order to delineate the structural origin of hsfsh-r specificity precisely, we have cloned dkfsh-r cdna from donkey testis mrna by rt-pcr. transiently expressed dkfsh-r endowed cos-7 cells with both hslh/cg- and fsh-binding activity, as well as fsh-induced camp production. the deduced dkfsh-r amino acid sequence shares 96% identity with the hsfsh-r: not ... | 1997 | 9195473 |
| serodiagnosis of babesia equi infection--a comparison of dot-elisa, complement fixation test and capillary tube agglutination test. | the present study aimed to develop dot-elisa, complement fixation test (cft) and capillary tube agglutination test (cat) for serodiagnosis of babesia equi infection and to compare their sensitivity with each other. for this study, sequential serum samples were collected from four donkeys experimentally infected with b. equi up to 90 days post infection (p.i.). b. equi antigen was prepared from the blood of a donkey showing more than 80% parasitaemia. dot-elisa, cf and ca tests were standardized ... | 1997 | 9195726 |
| a quantitative morphological study of age-related changes in the donkey testis in the period between puberty and senium. | the testis of the donkey was used as a model to study age-related changes in the period between puberty and senium. from the age of 1.5 years to the middle of sexual maturity (5 to 6 years) a number of histophysiological features, all indicative of the spermatogenetic efficiency, increase continuously. then without a longer-lasting plateau of maximal performance these features undergo continuous retrogression. thus, the adult testis is an organ in permanent change. during its progressive period ... | 1997 | 9197920 |
| failure to establish chronic infection of the reproductive tract of the male horse with a south african asinine strain of equine arteritis virus (eav). | eight sexually mature horse stallions were inoculated intranasally with a south african asinine strain of eav, a strain that was isolated from the semen of a donkey carrier. all horses developed fever, with maximum rectal temperatures of 38.9-39.9 degrees c recorded 3-6 d post challenge. six horses showed very mild clinical signs of equine viral arteritis and two were asymptomatic. the virus was recovered from the nasopharynxes of six horses 2-7 d after inoculation, and from buffy-coat samples o ... | 1997 | 9204499 |