Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| therapeutic effect of oxfendazole on the intestinal phase of trichinella spiralis larvae. | 1984 | 6736695 | |
| immunomodulation by propionibacterium granulosum kp 45 and experimental trichinellosis in mice. | swiss mice were infected orally with 200 trichinella spiralis larvae and injected intraperitoneally with a potent immunomodulator (isolated cell walls of propionibacterium granulosum strain kp 45). in control mice 22 871 +/- 9913 larvae were found in the whole body, whereas in mice treated with p. granulosum kp 45 on the day of inoculation 9450 larvae +/- 8244 larvae were counted (p less than 0.01). immunomodulation 7 days before or 7 days after t. spiralis inoculation gave less pronounced effec ... | 1984 | 6741346 |
| trypanosoma musculi and trichinella spiralis: concomitant infections and selection for resistance genotypes in mice. | trypanosoma musculi infections were given to mice of different strains before, at the same time, and after an infection with 400 trichinella spiralis. examined parameters of the host response to t. spiralis were worm rejection, antifecundity responses, development of immunological memory, and muscle larvae burden. after dual infection, each mouse strain showed characteristic effects on resistance to t. spiralis. this was due to a dynamic interaction between the genes controlling rejection of t. ... | 1984 | 6745385 |
| trypanosoma musculi with trichinella spiralis or heligmosomoides polygyrus: concomitant infections in the mouse. | inbred mice infected with trypanosoma musculi displayed wide variations in peak blood parasitemia. the most susceptible mice were c3h and a strain, while balb/c, c57b1/6, and the related congenic b10 strains were the most resistant. the effect of an intestinal infection with either trichinella spiralis or heligmosomoides polygyrus on proliferation of t. musculi was investigated. t. spiralis infections given at the same time or up to 45 days before a t. musculi infection always caused an increase ... | 1984 | 6745389 |
| genetic control of immunity to trichinella spiralis infections of mice. hypothesis to explain the role of h-2 genes in primary and challenge infections. | h-2 congenic strains of mice were compared for their ability to expel t. spiralis infections from the small intestine and for their ability to limit the reproduction of adult female worms. b10.m mice (h-2f) expelled both primary and challenge infections more quickly than did the strains b10.q(h-2q) and b10.br(h-2k). during a primary infection, expulsion of worms from b10.m mice began before day 9 post-infection and worm counts differed significantly (p less than .05 student's t-test) from counts ... | 1984 | 6423524 |
| [possibilities of survival of various parasites in meat and meat products]. | the consumption of meat and meat products may result in parasitic infection in man. trichinella spiralis infection can be very dangerous, but this parasite is now rarely encountered in the netherlands; the same applies to taenia solium. taenia saginata is regularly detected but is not particularly harmful to the consumer. the pathogenicity of sarcocystis bovihominis and sarcocystis suihominis for humans remains uncertain. toxoplasma gondii may give rise to severe problems, particularly congenita ... | 1984 | 6427970 |
| a single gene determines rapid expulsion of trichinella spiralis in mice. | in rats and some inbred mouse strains, one immune response, rapid expulsion, confers up to 95% protection against a challenge infection with trichinella spiralis. strain analysis in mice has shown that only three inbred strains, all originating from swiss-line mice at the national institutes of health, bethesda, md., express rapid expulsion. crosses between responder strain mice (nfr/n) and nonresponders (c3h/hej or b10 x br) have indicated that rapid expulsion is dominant and autosomal (bell et ... | 1984 | 6429049 |
| effect of size of trichinella spiralis (nematode) infections on glucose and ion transport in the rat intestine. | an in vivo perfusion technique, using 3 intestinal loops representing the anterior, mid and posterior regions of the rat small intestine, was used to determine intestinal glucose uptake 5 days after infection with trichinella spiralis. at high levels of infection (3,000 and 6,000 larvae/rat) net glucose absorption by the intestinal mucosa was significantly impaired in all regions of the small intestine when compared to uninfected controls. at low levels of infection (50 larvae/rat) glucose uptak ... | 1984 | 6438293 |
| preconditioning of trichinella spiralis nativa larvae in musculature to low temperatures. | preconditioning of a trichinella spiralis nativa isolate in ferret and fox musculature was carried out by freezing at -15 degrees c from 0 to 322 days prior to low temperature refrigeration at -32 degrees c. a limited number of preconditioned samples of infected fox musculature was also refrigerated at -45 degrees c. preconditioned larvae were appreciably more resistant than those subjected to the low temperatures directly. under the conditions of this investigation, the longer the period of pre ... | 1984 | 6441341 |
| protection against trichinella spiralis induced by a monoclonal antibody that promotes killing of newborn larvae by granulocytes. | mouse monoclonal antibodies directed against biochemically defined surface antigens of trichinella spiralis were selected and tested for their ability to destroy parasites in vivo and in vitro. one of these (nim-m5; igg1), which recognised a surface component of approximately 64 k molecular weight in newborn larvae (nbl), bound to a surface component of this stage (as shown by fluorescence), and mediated the adherence of rodent eosinophil leucocytes to the surface of living nbl. following cell a ... | 1984 | 6377201 |
| in vivo and in vitro responses to sheep erythrocytes by lymph node cells from mice with trichinellosis. | mice infected with trichinella spiralis for 20 days have decreased numbers of plaque forming cells (pfc) in the draining lymph nodes following subcutaneous immunization with sheep erythrocytes. however, when immunized in vitro, lymph node cells from infected mice generate more pfc than normal controls. splenectomy has no effect on suppression observed in vivo. there is a large increase in the proportion and numbers of b cells in the lymph nodes of infected mice, and these cells may account for t ... | 1984 | 6380831 |
| immunomodulation by nematodes: a review. | there is current evidence that infections with trichinella spiralis, ascaris suum, nippostrongylus brasiliensis, nematospiroides dubius (syn. heligmosomoides polygyrus) and diverse filariae affect the immune responsiveness of their hosts. t. spiralis, or its extracts, can depress or enhance the heterologous humoral or cell-mediated immunities, and affect macrophage activity or the response to other invaders. these effects are induced by products of the migratory and early muscle larvae and appea ... | 1984 | 6382784 |
| comparison of monoclonal antibody-based competitive and indirect enzyme-linked immunosorbent assays for the diagnosis of swine trichinosis. | a competitive enzyme-linked immunosorbent assay (elisa) for the detection of swine trichinosis has been developed using a biotinylated monoclonal antibody and an avidin-enzyme conjugate. the assay is based on competitive binding between swine serum antibodies and a monoclonal antibody specific for an antigenic determinant present on proteins from trichinella spiralis excretory-secretory products with molecular weights of 45,000, 49,000, and 53,000. the competitive elisa reliably detected pigs in ... | 1984 | 6385467 |
| expulsion of trichinella spiralis from the intestine of w/wv mice reconstituted with haematopoietic and lymphopoietic cells and origin of mucosal mast cells. | the involvement of haematopoietic and lymphopoietic cells in the expulsion of trichinella spiralis from the intestine of mice, and the origin of the subepithelial mast cells (smcs) and the globule leucocytes (gls) were determined using smc- and gl-deficient wbb6f1-w/wv and wcb6f1-s1/s1d mice. on day 12 after infection with t. spiralis, smcs and gls appeared in w/wv mice injected with spleen and bone marrow cells from normal +/+ mice, but not in w/wv mice injected with thymus, mesenteric lymph no ... | 1984 | 6386673 |
| distribution, prevalence and intensity of trichinella spiralis infection in furbearing mammals of pennsylvania. | tongues from 1,170 raccoons, 384 opossums, 201 muskrats, 168 foxes (red and grey), 51 skunks, and 17 mink from pennsylvania were examined for trichinella spiralis larvae by peptic digestion. the overall prevalence of t. spiralis infection was 3.2%, varying in the carnivores from 2.6% in raccoons to 15.1% in red foxes; no muskrats were infected. prevalence varied regionally, being higher west of the susquehanna river, where, in the ridge and valley province, it reached 9%. the larval densities va ... | 1984 | 6387078 |
| the occurrence of antibodies to hidden and exposed determinants of surface antigens of trichinella spiralis. | mice were infected per os with trichinella spiralis and their lymphocytes were removed and fused with mouse myeloma cell line p3 x 63ag8653p3 for the selection of monoclonal antibodies to biochemically defined, stage-specific surface antigens of 3 parasite developmental stages: muscle larvae, adults and newborn larvae. two separate antibodies against a defined single surface antigen of each stage were isolated. in each separate case the pair of monoclonal antibodies precipitated the same compone ... | 1984 | 6201803 |
| schistosoma mansoni, s. haematobium, and s. japonicum: identification of genus- and species-specific antigenic egg glycoproteins. | immunoreactive egg glycoproteins of schistosoma mansoni, s. haematobium, and s. japonicum which are genus- and species-specific, or react with sera of patients infected with other parasites, have been identified. egg proteins were labeled with iodine-125, and the concanavalin a-binding glycoproteins were immunoprecipitated with sera of patients infected with one of four species of schistosoma or trichinella spiralis, taenia solium, echinococcus granulosus, entamoeba histolytica, or wuchereria ba ... | 1984 | 6209162 |
| the influence of some anthelmintics on the bioenergetic metabolism of trichinella spiralis and trichinella pseudospiralis. | 1984 | 6466367 | |
| oxfendazole: regimen-dependent expression of drug efficacy against trichinella spiralis. | 1984 | 6469447 | |
| development of the male genitalia of trichinella spiralis during the enteral phase of infection in the mouse: an sem study. | 1984 | 6470896 | |
| rapid expulsion of trichinella spiralis in suckling rats. | orally administered trichinella spiralis muscle larvae were rapidly expelled by rat pups suckling an immune dam. the immunity was delivered in the milk; substantial resistance was conferred on normal rat pups suckled for only 24 hours by a trichinella-immune foster mother. the pups were protected by oral or systemic administration of specific serum antibodies. when infused into a normal lactating dam, these antibodies accumulated in the serum of her suckling pups. | 1984 | 6474191 |
| toxicity of bis(tri-n-butyltin)oxide in the rat. ii. suppression of thymus-dependent immune responses and of parameters of nonspecific resistance after short-term exposure. | to evaluate the functional significance of bis(tri-n-butyltin)oxide (tbto)-induced thymus atrophy, lymphocyte depletion in spleen and lymph nodes, lymphopenia, and increased serum igm and decreased igg concentrations, in vivo and in vitro function studies were performed for specific and nonspecific resistance. weaned male rats were fed diets containing 0, 20, or 80 mg tbto/kg for at least 6 weeks. regarding the thymus-dependent immunity, delayed-type hypersensitivity reactions to ovalbumin as we ... | 1984 | 6474470 |
| tissue and blood eosinophilia in mast cell-deficient sl/sld mice infected with trichinella spiralis. | 1984 | 6482120 | |
| immunodiagnosis of giardiasis by elisa and studies on cross-reactivity between the anti-giardia lamblia antibodies and some heterologous parasitic antigens and fractions. | specific antibodies against giardia lamblia in 92 sera from asymptomatic carriers were investigated by elisa. using cyst antigen, antibodies were detected in 91 of the sera, by means of alkaline phosphatase-labelled antispecies immunoglobulin. the e/405nm values of the positive sera ranged between 0.350 and 1.700, whereas in the 78 control sera they were 0.270 +/- 0.220. cross-reactivity between the anti-g. lamblia antibodies and 18 heterologous parasitic antigens and fractions was also studied. ... | 1984 | 6486935 |
| stage-specific antigens of trichinella spiralis. | infective larvae, adults and newborn larvae of trichinella spiralis were surface labelled with radioactive iodine, and the surface material was solubilized in the mild detergent sodium deoxycholate. the radio-isotope labelled products were stage-specific glycoproteins that were few in number (2-4 components) and antigenic in infected mice and rats. antibodies synthesized in infected animals against these biochemically defined surface antigens may or may not interact with the surface of the livin ... | 1984 | 6493807 |
| evasion of the immune response: survival within low responder individuals of the host population. | it is proposed that, for many species of parasites, evasion of the host immune response may be achieved passively through enhanced survival within host individuals that have a genetically determined low responsiveness to infection. evasion by this means may contribute significantly to continued transmission of infection in man and domestic animals and influence the severity of pathology. low responsiveness plays an important role in determining over-dispersion of parasites within host population ... | 1984 | 6493808 |
| h-2-controlled, dose-dependent suppression of the response that expels adult trichinella spiralis from the small intestine of mice. | h-2 congenic strains of mice expressing the h-2k, h-2q or h-2f haplotype were tested for their ability to expel trichinella spiralis from the gut following infection with either 100, 150, 200, 400, 500, or 600 l1 infective larvae. h-2q and h-2f mice expelled worms more quickly than h-2k mice when 100-200 l1 larvae were given, but this h-2-controlled effect was much reduced when mice received 400 l1 larvae, and completely eliminated when 500 or 600 l1 larvae were given. the observed dose-dependen ... | 1984 | 6500629 |
| immune rejection of trichinella spiralis infective larvae in the guinea pig. | 1984 | 6502363 | |
| mucosal mast cells are functionally active during spontaneous expulsion of intestinal nematode infections in rat. | infestation of the gastrointestinal tract by parasitic nematodes is invariably associated with mucosal mastocytosis, which is a thymus-dependent phenomenon in parasitized rats, and is adoptively transferable with a t cell-enriched population of thoracic duct lymphocytes. when derived by in vitro culture, mucosal mast cells (mmc) arise from a bone marrow precursor after stimulation by t cell-derived factors. in rats infected with the nematode trichinella spiralis, mucosal mastocytosis is temporal ... | 1984 | 6504156 |
| altered host resistance to trichinella spiralis infection following subchronic exposure to diethylstilbestrol. | the effects of subchronic exposure to diethylstilbestrol on the host response to infection with trichinella spiralis were examined in adult b6c3f1 mice. expulsion of adult trichinella from the small bowel, intestinal inflammation and delayed hypersensitivity responses to trichinella antigens in artificially sensitized mice were investigated. administration of 8 micrograms/g of diethylstilbestrol for five consecutive days beginning on days -5,0, +3 or +8 of infection inhibited adult worm expulsio ... | 1984 | 6511144 |
| tissue eosinophil numbers and phospholipase b activity in mice infected with trichinella spiralis. | 1984 | 6511177 | |
| the intestinal mast cell response to trichinella spiralis infection in mast cell-deficient w/wv mice. | the intestinal mast cell response and lymphoblast activity, as measured by the incorporation of 3h-thymidine into mesenteric lymph node cells (mln) of wbb6f1-w/wv(w/wv) mice, their normal congenic littermates (+/+) and c57bl/6j mice, were compared after infection with trichinella spiralis. marked and similar blast cell activity and an increase in number of cells were observed in the mln of infected w/wv and c57bl/6j mice 7 and 15 days p.i. in contrast to c57bl/6j mice, primary t. spiralis intest ... | 1984 | 6512640 |
| running responses of trichinella spiralis-infected cd-1 mice. | male cd-1 mice inoculated by gavage with 400 larvae of trichinella spiralis exhibited an increased latency of the wall-seeking response as well as a decline in running speed and the distance travelled to the first stop when compared with normal controls or animals inoculated with only 100 larvae. doses of 200 larvae of this parasite affected running speed and distance travelled but not the advent of the running response. such changes in behaviour may reduce the competitive fitness of the host an ... | 1984 | 6514382 |
| [first finding of trichinella spiralis in the diaphragm of a cadaver in zacatecas. preliminary note]. | 1984 | 6515468 | |
| a freeze-fracture study of the surface of the infective-stage larva of the nematode trichinella. | the surface layers of the cuticle of the infective, first-stage larva of the nematodes trichinella spiralis and t. spiralis var. pseudospiralis have been studied by means of the freeze-fracturing technique. no obvious differences between the two nematodes were found. a double-layered structure covers the cuticle. its outermost layer consists of particles embedded in an amorphous matrix; its inner layer is composed of a sheet of fine filaments which may be composed of globular subunits. this uniq ... | 1984 | 6515646 |
| the effect of the intestinal phase of trichinella spiralis on the open-field behavior of mice. | the study assessed the impact of the adult phase of trichinella spiralis on the open-field behavior of mice. thiabendazole treatment was used to chemosterilize adult worms in vivo. untreated mice exhibited a marked decline in their ambulatory and exploratory activities (from day 14 post-infection until the end of the experiment). in contrast, infected mice treated with thiabendazole did not exhibit any behavioral alterations. this suggested that the adult phase of t. spiralis did not contribute ... | 1984 | 6527189 |
| long term duration of the rapid rejection response in rats infected with trichinella spiralis. | 1984 | 6527193 | |
| the site of ecdysis of the l1 larva of trichinella spiralis. | 1984 | 6527198 | |
| interbreeding and fecundity of a single pair of two strains of trichinella spiralis in mice. | 1984 | 6527466 | |
| a comparison between the efficacy of thiabendazole and febantel on experimental trichinellosis in rats. | febantel at a dose level of 5 mg kg-1 had a lethal efficacy of 54.1% against the adult worm and 69.5% against the migrating larvae of trichinella spiralis in rats. when administered at 15 mg kg-1, it demonstrated lethal effects of 94.4, 92.5 and 74.7% against adult, young and encysted larvae, respectively. infected rats treated with 300 mg kg-1 died. thiabendazole had efficiencies of 96.4 and 83.3% against intestinal and migrating early muscle phases, respectively, and was 42% effective against ... | 1984 | 6538721 |
| the cellular ige response of rodents to infection with nippostrongylus brasiliensis, trichinella spiralis and schistosoma mansoni. | the ige response at the cellular level to helminthic infection was studied in balb/c mice inoculated with the infective larvae of the nematodes nippostrongylus brasiliensis (nb) or trichinella spiralis (ts) or with the cercariae of the trematode schistosoma mansoni (sm). changes in mesenteric lymph node (mln) cell number, cell surface(s) igd, igm, ige and thy-1.2 and intracytoplasmic (c) ige were recorded. in addition, a comparable study was conducted in rats infected with nb. at 11 days after i ... | 1984 | 6540929 |
| immunization of swine against trichinella spiralis. | swine were immunized with partially purified stichosome antigens derived from trichinella spiralis muscle larvae. in 3 trials, 500 to 600 micrograms of the solubilized particle component (s3) induced moderate levels of resistance to challenge inoculation; the percent reduction in larvae per gram of muscle ranged from 43 to 55. the effect of immunization with s3 on pig intestinal expulsion of adult worms was determined in one experiment and the results indicated that s3 immunization was only weak ... | 1984 | 6541840 |
| trichinella spiralis: genetics of worm expulsion in inbred and f1 mice infected with different worm doses. | the nematode trichinella spiralis is rejected from the intestine at a time that is characteristic for each inbred strain of mouse. previous work (r. g. bell et al. 1982a) had empirically identified strong, intermediate, and weak phenotypes (nfr, c3h/he, and c57b1/10 mice, respectively) in mice infected with 400 muscle larvae. it is shown that this classification applies to another eight inbred strains: swr, dba/2, dba/1, lp, bub/bn--all intermediate, and nzb/bin, c57l, a, and mus molossinus--all ... | 1984 | 6542023 |
| comparison of three methods for detection of prolonged experimental trichinellosis in pigs. | three methods were employed for the diagnosis of porcine trichinellosis. the pooled sample digestion method and trichinoscopy served as european community (ec) reference techniques, whereas the reliability of the enzyme linked immunosorbent assay (elisa) was tested by 11 laboratories of the european community and sweden. three groups of 6 piglets each were orally inoculated with 50, 150 and 1500 trichinella spiralis larvae into each animal. another group of 6 animals served as a non-infected con ... | 1984 | 6543048 |
| husbandry, parasitic and other diseases as factors in the reliability of the enzyme linked immunosorbent assay (elisa) for detection of trichinellosis in pigs. | serum samples were obtained from pigs originating from specified pathogen-free farms, large industrialised farms and small conventional farms. all animals proved to be free of trichinella spiralis by a pooled sample digestion method. careful meat inspection studies on parasitic infections other than trichinellosis, and other inflammatory reactions were recorded and used for subdivision of the animals in different groups. it was concluded that animal husbandry, parasitic infections, or inflammato ... | 1984 | 6543049 |
| myocarditis caused by trichinella spiralis. | 1984 | 6546336 | |
| myeloperoxidase is more efficient than eosinophil peroxidase in the in vitro killing of newborn larvae of trichinella spiralis. | myeloperoxidase (mpo) and eosinophil peroxidase (epo) catalyse the formation of hypochlorite (ocl-) from chloride ions (ocl-) and hydrogen peroxide (h2o2). ocl- proved to be highly toxic for trichinella spiralis newborn larvae (nbl) in in vitro assays. using purified human mpo and epo it was found that even at neutral ph both enzymes under appropriate conditions are able to kill nbl. the rate at which ocl- is produced is much lower in the epo- than in the mpo-mediated reaction. this difference i ... | 1984 | 6321330 |
| [inhibition of plasmodium berghei in rats infested with strongyloides ratti or trichinella spiralis; role of high blood corticosterone in reaction to the development of helminths]. | the plasma corticosterone induced in the rat by the development of strongyloides ratti or trichinella spiralis reaches a sufficient level of intensity to determine reticulocytopenia. the latter is linked chronologically to the inhibition of parasitemia in plasmodium berghei, which occurs when this protozoa develops at the same time as the nematodes, and seems to be the causal factor. this hypothesis may be verified by replacing the helminths with the corticotropic action of a.c.t.h. which causes ... | 1985 | 3002223 |
| [bioenergy metabolism in larvae of trichinella spiralis spiralis and trichinella spiralis pseudospiralis]. | 1985 | 2933879 | |
| stage-specific antigens of trichinella spiralis. | 1985 | 2862082 | |
| the effects of histamine and an antihistamine on trichinella spiralis and on trichinous enteritis in the host. | 1985 | 2863228 | |
| trichinella spiralis: immunization of mice using monoclonal antibody affinity-isolated antigens. | an antigen epitope was identified from the excretory-secretory products of trichinella spiralis first-stage larvae using monoclonal antibodies, and the glycoprotein antigens bearing this epitope (ts.49 and ts.53) were isolated from the crude excretory-secretory preparation by affinity chromatography. in immunization experiments carried out in mice, antigen priming with ts.49 and ts.53 resulted in a reduction of muscle larvae resulting from a challenge infection at a level comparable to priming w ... | 1985 | 2581801 |
| trichinosis in thailand during 1962-1983. | various studies on trichinosis in thailand since the first outbreak in 1962 up until 15 july 1983 are reviewed. there were 67 outbreaks, affecting 2,792 persons and resulting in 85 deaths. most of the outbreaks occurred in the north of the country which is mountainous. hilltribe pigs and wild boars were the common sources of human infection. incidence of trichinella spiralis larvae in dog meat in the area favoring of dog meat consumption is cautioned to create a public health problem in the futu ... | 1985 | 3836212 |
| efficacy of ivermectin against trichinella spiralis in mice. | 1985 | 3840934 | |
| l3t4-positive t lymphoblasts are responsible for transfer of immunity to trichinella spiralis in mice. | the characteristics of lymphocyte subpopulations involved in mediating immunity to the intestinal nematode trichinella spiralis in vivo have been examined using adoptive transfer in conjunction with accurate cell-sorting and cell-depletion techniques. positive selection of cell subsets, using facs sorting and velocity sedimentation at unit gravity, confirm that rapidly dividing t blasts are the major population that mediates expulsion of the worm from the gut. furthermore, cell-depletion studies ... | 1985 | 3876979 |
| the effect of dimethylnitrosamine on host resistance and immunity. | adult female b6c3f1 mice were injected ip with 0.2 ml phosphate-buffered saline (pbs) only or pbs containing 1.5, 3, or 5 mg dimethylnitrosamine (dmn)/kg body wt daily for 14 days. on day 16, mice were evaluated for changes in immune status as measured by the antibody response to sheep red blood cells (srbcs), blastogenesis to t- and b-cell mitogens, natural killer (nk) cell function, delayed hypersensitivity, and alveolar macrophage (am) bactericidal activity; and for changes in host resistance ... | 1985 | 3883572 |
| superiority of the elisa technique over parasitological methods for detection of trichinellosis in slaughtered pigs in canada. | trichinoscopic examination, pepsin-hydrogen chloride digestion and the elisa assay were used in an epidemiological survey for the presence of trichinella spiralis in slaughterhouse pigs over a period of 12 months. the first two methods failed to detect any cases of trichinellosis in sows aged between two to three years. the elisa assay, however, detected a 2.5% prevalence amongst the 2046 blood specimens tested. foci of t. spiralis are localized in certain regions only. the discrepancy between t ... | 1985 | 3886108 |
| antigen production by encysted muscle larvae of trichinella spiralis. | the production of excretory-secretory antigens by encysted muscle larvae of trichinella spiralis has been investigated immuno-histochemically using an antiserum raised by infection in rabbits and purified both before and after conjugation by ion-exchange chromatography. the specificity of the antibody for excretory-secretory products was demonstrated by the pattern of staining of live worms in vitro and the failure of the labelled antibody to stain dead, non-metabolizing worms. using this labell ... | 1985 | 3886777 |
| effects of macrophage activity on the antibody-dependent cytotoxicity against trichinella spiralis newborn larvae: an in vitro cytotoxicity and ultrastructural study. | the effect of the activity of macrophages on the antibody-dependent cytotoxicity against trichinella spiralis newborn larvae was studied in vitro. macrophages present in peritoneal exudates from mice genetically selected for high and low antibody production (hl and ll, respectively) showed an inverse cytotoxic effect. cells from hl mice were ineffective, whereas cells from ll mice had a very high killing capacity. ultrastructural studies of cells after incubations of up to 36 h supported these o ... | 1985 | 3887792 |
| preparation of antibodies to guinea pig ige and its use for enzyme-linked immunosorbent assay of ige antibodies. | guinea pigs were infected with trichinella spiralis. a pooled serum from the infected guinea pigs was fractionated by deae-cellulose column chromatography and sephadex g-200 gel filtration. an ige-rich fraction was injected into rabbits. the antiserum from the rabbits, after appropriate absorption with a normal guinea pig plasma, formed a precipitin line in immunoelectrophoresis and in immunodiffusion against a guinea pig serum containing ige antibodies to ovalbumin. uptake of 125i ovalbumin was ... | 1985 | 3894244 |
| glycosidases of trichinella spiralis. | the exoglycosidases beta-n-acetyl-d-glucosaminidase, beta-n-acetyl-d-galactosaminidase, alpha-1-fucosidase, alpha-d-glucosidase and alpha-d-mannosidase, and a non-specific acid phosphohydrolase are present at high levels in extracts of adult and muscle-stage (l1) trichinella spiralis and at lower (5-30-fold) levels in extracts of the newborn larvae. the enzyme activities from the l1 extract were characterized. all displayed maximum activity at acid ph. beta-n-acetyl-d-glucosaminidase and beta-n- ... | 1985 | 3897859 |
| [150th anniversary of the discovery of the parasite trichinella spiralis. aspects in the evolution of our knowledge of t. spiralis and trichinelliasis]. | 1985 | 3903953 | |
| eosinophil adherence to infective larvae of trichinella spiralis: quantification and modulation. | eosinophils play an important role in host defence against helminthic infection. a quantitative system for studying the interactions of eosinophils and a large non-ingestible target was developed using infective muscle-stage larvae of trichinella spiralis. at 37 degrees, this antibody-dependent system provided a reliable, reproducible index of eosinophil function. adherence was equivalent under aerobic and anaerobic conditions. no significant differences were noted between peritoneal exudate eos ... | 1985 | 3932192 |
| immunity to onchocerca lienalis microfilariae in mice. ii. effects of sensitization with a range of heterologous species. | the model of onchocerca lienalis microfilariae (mff) injected into cba and t.o. strains of mice has been used to examine immunity to skin-dwelling microfilariae following exposure to a range of species of helminths. mice which had received a primary infection with o. cervicalis mff were significantly resistant to challenge with o. lienalis mff (58% reduction relative to challenge controls). immunization with the uterine contents (eggs and mff) of o. lienalis, o. gutturosa or o. volvulus conferre ... | 1985 | 3937854 |
| effects on trichinella spiralis of host responses to purified antigens. | purification of two antigens (48-kilodalton polypeptide and a group with major subunits of 50 and 55 kilodaltons) from the infective larvae of the parasitic nematode trichinella spiralis was recently reported. immunization of mice with either of these antigens induces strong resistance to a subsequent challenge infection. in the study reported here the mechanism of this resistance was investigated by monitoring the parasite's life cycle in mice immunized with the antigens. immunized mice were ab ... | 1985 | 3969571 |
| anaphylactic-like reaction of small intestinal epithelium in parasitized guinea-pigs. | antigens derived from trichinella spiralis were used to challenge, in vitro, sensitized jejunum from infected guinea-pigs while monitoring ion transport properties of the tissue. antigen challenge resulted in dose-dependent increases in trans-epithelial electrical potential difference and short circuit current. both antigen-stimulated electrical alterations and schultz-dale contractions were demonstrated in small intestinal tissue after the passive transfer of immune serum containing anti-trichi ... | 1985 | 3972439 |
| infectivity of canadian isolates of trichinella spiralis nativa for swine, rats and carnivores. | the infectivity of trichinella spiralis nativa isolates from widely separated geographic areas of canada was determined by feeding infected musculature to swine, laboratory rats and carnivores (cats, foxes, ferrets). low infectivity for swine and rats and high infectivity for carnivores were observed. light infections were established in four of 16 swine examined between 25 and 53 days postinfection. feeding of infected porcine musculature to ferrets demonstrated that trichinella spiralis nativa ... | 1985 | 3986683 |
| prevalence of trichinosis in southern louisiana swine. | after an outbreak of human trichinosis in louisiana involving 45 cases and 1 death in 1979 and 1980, a survey of pigs killed in 21 selected small slaughterhouses in southwestern louisiana was conducted from november 1980 to september 1981. the sera from 1,225 pigs were examined for trichinella antibodies using an enzyme-linked immunosorbent assay (elisa); 1,223 diaphragms were subjected to peptic digestion and examined for the presence of trichinella spiralis larvae. one diaphragm (0.08%) was fo ... | 1985 | 3994113 |
| trichinella spiralis: acquired immunity in swine. | the ability of domestic pigs to develop protective immunity to trichinella spiralis in response to inoculation with different doses of muscle larvae was assessed. adult worms developing from the inoculations of 112, 500, and 10,000 larvae were expelled from the intestine about 6 weeks after inoculation. inoculation with 25,000 larvae, however, resulted in more rapid intestinal worms expulsion, indicating that gut expulsion is dose dependent. secondary expulsion also tended to be dependent upon p ... | 1985 | 3996525 |
| a freeze-fracture study of the digestive tract of the parasitic nematode trichinella. | freeze-fracture preparations of the esophagus and intestine of larvae and adults of the nematode trichinella spiralis illustrate the distribution of intramembranous particles in membranes of a number of cell types, and several specializations were found. esophageal glands are prominently linked by gap junctions, but gap junctions were not found between intestinal cells. muscle cells of the esophagus have rectilinear arrays of particles, thought to be points of adherence of the muscles to the eso ... | 1985 | 4012758 |
| trichinella spiralis: nonspecific resistance and immunity to newborn larvae in inbred mice. | the implantation and development of intravenously injected trichinella spiralis newborn larvae were examined in different strains of inbred mice by determining muscle larvae burden. this was compared to the numbers of muscle larvae that established after a natural infection during which a quantitative assessment of intestinal newborn larvae production was made. in most inbred strains of mice, newborn larvae do not all successfully implant in muscle. mice of the dba/1 strain are the most resistan ... | 1985 | 4018217 |
| the effect of dosage regimen on the efficacy of cambendazole against trichinella spiralis. | 1985 | 4030205 | |
| immune responses to trichinella pseudospiralis and trichinella spiralis in mice. | 1985 | 4030207 | |
| attempts to manipulate specific responses to induce resistance to schistosoma mansoni in kenyan baboons (papio anubis). | attempts were made to manipulate specific responses of baboons to protect them from infection with schistosoma mansoni. in experiment 1, eosinophilia was induced in naive baboons with trichinella spiralis larvae given intravenously before intraperitoneal injection of globulin fractions from s. mansoni-infected baboon sera and subsequent percutaneous exposure to s. mansoni cercariae. in experiment 2, baboons with 8- or 32-week-old primary s. mansoni infections received t. spiralis i.v. before an ... | 1985 | 4031458 |
| immunization with purified antigens protects mice from lethal infection with trichinella spiralis. | 1985 | 4032155 | |
| phosphatidylethanolamine methylation in intestinal brush border membranes from rats resistant to trichinella spiralis. | methylation of phospholipids is proposed as a mechanism to explain changes in properties of intestinal brush border membrane that coincide with development of immunity to the intraepithelial parasite, trichinella spiralis. methylation was measured by the incorporation of the [3h]methyl group from s-adenosyl-l-[3h]methyl methionine into phospholipids. at least two enzymatic components were detected that converted phosphatidylethanolamine to phosphatidylcholine. the first, designated methyltransfe ... | 1985 | 4033691 |
| evaluation of a purified schistosoma japonicum glycoprotein egg antigen for the immunodiagnosis of infection in man. | a purified glycoprotein, glycoprotein-2, which was isolated from schistosoma japonicum soluble egg antigen, was tested in an enzyme-linked immunoadsorbent assay for its potential as an immunodiagnostic reagent. using empirically-derived criteria of endpoint titer, 39/40 sera from s. japonicum-infected individuals were scored correctly as positive in a blind test. of these, 26/40 were from individuals who excreted fewer than 100 eggs per gram of stool. no cross reactions were observed with sera f ... | 1985 | 4038590 |
| adoptive transfer of immunity to trichinella spiralis in mice: generation of effective cells by different life cycle stages. | 1985 | 4039708 | |
| life-phase specific induction and expression of rapid expulsion in rats suckling trichinella spiralis-infected dams. | rat dams infected with 1000 trichinella spiralis muscle larvae, 4 weeks prior to breeding, provided their suckling offspring with immunity to challenge with 200 muscle larvae at 2 weeks of age. the immunity was expressed in the elimination of 75-99% of the challenge dose within 24 hr. the intestinal worm burden did not decline significantly after the initial expulsion. infected dams continued to protect their offspring during three breeding cycles, for as long as 26 weeks after infection. immuni ... | 1985 | 4040119 |
| in vitro cellular immune response in mice and rabbits immunized with trichinella spiralis antigen. | the possibility of using cell mediated immune response as a diagnostic tool has been investigated with t. spiralis laboratory/model. the feasibility of a in vitro microculture method to measure blast cell activity of lymphoblasts in mice experimentally infected with t. spiralis muscle larvae or immunized with soluble crude antigen and the lymphoproliferative response in immunized rabbits was demonstrated. the obtained results suggest that the cell mediated immune response from blood lymphocytes ... | 1985 | 4041244 |
| swine trichinosis in new england slaughterhouses. | diaphragms of 5,315 slaughter hogs from the new england states were examined for trichinella spiralis infection between june 22 and dec 22, 1983. thirty-nine hogs (0.73%) were infected. the infections were light, none exceeding 30 larvae/g of tissue, with an arithmetic mean of 4.7 larvae/g. infected hogs originated from 5 of 6 new england states. the widespread occurrence of porcine trichinosis in new england indicates that the small or part-time hog farming operations frequently found in this r ... | 1985 | 4051306 |
| [therapeutic efficacy of mebendazole and pyquiton against trichinella spiralis in mice]. | 1985 | 4053326 | |
| genetic control of immunity to trichinella spiralis in mice: capacity of cells from slow responder mice to transfer immunity in syngeneic and f1 hybrid recipients. | mice of the c57bl/10 (b10) strain are slow responders to infection with t. spiralis in terms of ability to expel worms from the intestine. compared with rapid-responder nih mice, infection stimulates a slower and reduced blast cell response in the draining mesenteric lymph node (mln). transfer of immune cells from the mln (mlnc) does not accelerate worm expulsion from naive b10 recipient mice, even though mlnc from this strain effectively transfer immunity to (b10 x nih) f1 recipients. in common ... | 1985 | 4054943 |
| the use of enzyme polymorphisms to identify genetic differences in the genus trichinella. | seven isolates of the genus trichinella were evaluated for their genetic identity and variability using starch gel electrophoresis. the various strains exhibited polymorphism in 8 of 11 enzyme systems tested and the strains could be segregated into at least 3 basic patterns for geographical isolates of trichinella: pigs, wild carnivores and trichinella spiralis var. pseudospiralis. there was also evidence for further differences between isolates from wild carnivores. the isozyme technique appear ... | 1985 | 4057010 |
| comparison of immune effects in mice immunized with trichinella spiralis adult and larval antigens. | 1985 | 4057011 | |
| trichinella spiralis in wild animals, cats, mice, rats and farmed fur animals in finland. | a total of 1 399 samples of wild animals, cats, mice, rats and fur animals were examined for trichinella larvae during the period 1.1.1982-30.6.1984. samples were obtained both from the problem area, were trichinella larvae had been found in pigs, and for comparison from the rest of finland. the frequency of the infection in wild carnivores, badgers, pine martens, raccoon dogs, foxes and wild mink was significantly higher in the problem area than in the rest of finland. | 1985 | 4059043 |
| prolonged expulsion of adult trichinella spiralis and eosinophil infiltration in mast cell-deficient w/wv mice. | eosinophil infiltrations were observed in the intestine and the muscle of both trichinella spiralis-infected (wbxc57bl/6)f1-w/wv mice and their littermates, wbb6f1-+/+, +/w, +/wv, almost to the same extent. w/wv mice did not show infiltration of subepithelial mast cells and globule leucocytes in response to t. spiralis infection. increased numbers of these cells were observed in their littermates. worms in w/wv mice were retained for longer periods than those in littermates. also, no difference ... | 1985 | 4067246 |
| compared genetic distance between trichinella spiralis and other trichinellae isolations by several immunization path-ways. | 1985 | 4072212 | |
| succinate dehydrogenase and fumarate reductase activity in trichinella spiralis and the mode of action of some anthelmintics. | 1985 | 4072213 | |
| comparative study of the fatty acids of trichinella spiralis, trichinella nativa and trichinella nelsoni. | 1985 | 4072214 | |
| [monoamine oxidase (mao) activity in the blood and homogenates of the spleen, mesenteric lymph node and small intestine of mice with experimental trichinella spiralis infection]. | 1985 | 4072215 | |
| [basophilic transformation of the muscle cells of mice during infection with trichinella spiralis larvae. i. intramuscular (synchronous) infection with larvae of various developmental stages]. | 1985 | 4072219 | |
| [value of various microelements in the course of trichinella spiralis infection in mice]. | 1985 | 4072221 | |
| effect of clofibrate on the endogenous cycle of trichinella spiralis and trichinella nelsoni. | 1985 | 4072224 | |
| vaccination against the gastrointestinal helminths trichinella spiralis and hymenolepis nana: relationship between routes of immunization and effective protection. | mice immunized with antigens derived from trichinella spiralis muscle larvae or hymenolepis nana embryos were found to be protected against the homologous challenge infection. intraperitoneal route was found the most effective in stimulating protection compared with subcutaneous or intravenous administration. even a single dose of antigen administered intraperitoneally stimulated a significant protection. the results of our experiments confirm that the route of administration has a noteworthy in ... | 1985 | 4091979 |
| cell injury caused by trichinella spiralis in the mucosal epithelium of b10a mice. | most of the mucosal epithelium in the anterior small intestine of b10a mice infected with trichinella spiralis showed no cytopathology. however, isolated foci of damaged cells or dense masses of multinucleate cytoplasm were seen in the crypt-villus junction, or the base of the villi. cells occupied by the nematode ranged from a nearly normal appearance, showing only compressed nuclei and organelles, to progressive inflation and vesiculation of endoplasmic reticulum, loss of terminal web and henc ... | 1985 | 4093809 |
| the effects of trichinella spiralis infection of pregnant mice on the future behavior of their offspring. | trichinella spiralis infections in pregnant mice had both pre- and post-natal effects on the future behavior of the offspring. young, born of infected and reared by uninfected mothers as well as young born of uninfected and reared by infected mothers, were behaviorally distinguishable from controls that were born and reared by uninfected mothers when assessed by their tendency to emerge from their home cages into an unfamiliar open-field arena. the pre- and post-natal effects appeared to be addi ... | 1985 | 4093811 |
| a survey of trichinella spiralis in wild carnivores in southwestern quebec. | in 1982-1983, 144 muscle samples from 11 different species of wild carnivores from southwestern quebec, canada, were examined for the presence of trichinella spiralis larvae, using direct microscopic examination and the peptic digestion method. two red foxes (vulpes vulpes) (out of 29) and one pine marten (martes americana) (out of 56) were found positive, giving a 2.1% prevalence for the whole sample. | 1985 | 17422546 |
| comparison of the microbial populations of retail beef and pork. | retail samples of beef mince, beef rump, pork mince and pork rump were enumerated for total plate count, coliforms, escherichia coli, staphylococcus aureus, salmonella and trichinella spiralis. total plate counts were similar for all samples; however, the populations of coliforms and staphylococcus aureus were higher in minced meats than in rumps (p < 0·05). minced pork was found to carry higher loads of staphylococcus aureus than any other meat (p < 0·05). salmonella and trichinella spiralis we ... | 1986 | 22055645 |
| trichinella spiralis: newborn larval migration route in rats reexamined. | the route by which trichinella spiralis newborn larvae migrate from the small intestine to striated muscle was studied in inbred ao and random-bred sprague-dawley rats. newborn larvae were quantitatively recovered from the thoracic duct lymph, peritoneal cavity, and hepatic portal vein blood during the course of a primary infection with 4000 muscle larvae. the total recovery of newborn larvae assessed in this manner was compared with the number of muscle larvae in control rats receiving the same ... | 1986 | 3943594 |