Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| solubilization of insoluble zinc compounds by gluconacetobacter diazotrophicus and the detrimental action of zinc ion (zn2+) and zinc chelates on root knot nematode meloidogyne incognita. | to examine the zinc (zn) solubilization potential and nematicidal properties of gluconacetobacter diazotrophicus. methods and results: atomic absorption spectrophotometer, differential pulse polarography and gas chromatography coupled mass spectrometry were used to estimate the total zn and zn(2+) ions and identify the organic acids present in the culture supernatants. the effect of culture filtrate of zn-amended g. diazotrophicus pal5 on meloidogyne incognita in tomato was examined under gnotob ... | 2007 | 17309498 |
| the mi-1-mediated pest resistance requires hsp90 and sgt1. | the tomato (solanum lycopersicum) mi-1 gene encodes a protein with putative coiled-coil nucleotide-binding site and leucine-rich repeat motifs. mi-1 confers resistance to root-knot nematodes (meloidogyne spp.), potato aphids (macrosiphum euphorbiae), and sweet potato whitefly (bemisia tabaci). to identify genes required in the mi-1-mediated resistance to nematodes and aphids, we used tobacco rattle virus (trv)-based virus-induced gene silencing (vigs) to repress candidate genes and assay for nem ... | 2007 | 17351050 |
| the root-knot nematode producing galls on spartina alterniflora belongs to the genus meloidogyne: rejection of hypsoperine and spartonema spp. | root-knot nematodes are a major group of plant-parasitic nematodes, but their sister group within the tylenchida remains to be identified. to find the sister group and for any investigation of the evolutionary biology of the genus meloidogyne, it would be useful to identify the most basal species within meloidogyninae. meloidogyne spartinae, a root-knot nematode parasitic on cordgrass (spartina spp.), constitutes a potentially interesting early diverging (or at least highly divergent) root-knot ... | 2007 | 19259481 |
| the effect of animal manures on susceptibility of cowpea var. moussa local to infection by root-knot nematode; meloidogyne javanica treub. | the effect of three different types of animal manures namely; poultry, goat and cow dung on the susceptibility of cowpea (vigna unguiculata (l.) walp) var moussa local to root-knot nematode infection caused by meloidogyne javanica was tested in a replicated pot experiment. manured plants were inoculated with 2000 eggs of m. javanica while uninoculated manured plants served as control for each manure type and unmanured but inoculated plants as general control. the result showed that unmanured and ... | 2007 | 19090213 |
| transfer and development of pasteuria penetrans. | pasteuria penetrans isolate p-20 has been attributed as the cause of soil suppressiveness to peanut root-knot nematode in florida. in this study, p. penetrans was transferred from a suppressive site to a new site and established by growing susceptible hosts to the peanut root-knot nematode during both summer and winter seasons. when two soil fumigants, 1,3-dichloropropene (1,3-d) and chloropicrin, were applied broadcast at the rate of 168 liters/ha and 263 kg/ha, respectively, the bacterium was ... | 2007 | 19259476 |
| control of meloidogyne chitwoodi in potato with shank-injected metam sodium and other nematicides. | metam sodium (ms) is often applied to potato fields via sprinkler irrigation systems (water-run, wr) to reduce propagules of soil-borne pathogenic fungi, particularly verticillium dahliae, to prevent yield loss from potato early dying disease. however, this procedure has not been effective for controlling quality defects in tubers caused by columbia root-knot nematode (meloidogyne chitwoodi). in five trials from 1996 to 2001, application of ms by soil shank injection (sh) provided better control ... | 2007 | 19259485 |
| a new root-knot nematode parasitizing sea rocket from spanish mediterranean coastal dunes: meloidogyne dunensis n. sp. (nematoda: meloidogynidae). | high infection rates of european sea rocket feeder roots by an unknown root-knot nematode were found in a coastal dune soil at cullera (valencia) in central eastern spain. morphometry, esterase and malate dehydrogenase electrophoretic phenotypes and phylogenetic trees demonstrated that this nematode species differs clearly from other previously described root-knot nematodes. studies of host-parasite relationships showed a typical susceptible reaction in naturally infected european sea rocket pla ... | 2007 | 19259488 |
| effects of nematicides and plant resistance on white clover performance and seasonal populations of nematodes parasitizing white clover in grazed pasture. | root-infecting nematodes are a major cause of white clover, trifolium repens, not reaching its potential in new zealand pastures. resistance and/or tolerance are the preferred control options. greenhouse-based, recurrent selection programs have developed resistance to meloidogyne trifoliophila and heterodera trifolii, and a field-based program has developed tolerance. lines from these programs were compared with commercial cultivars as controls in a series of field trials at four sites over 4 ye ... | 2007 | 19259502 |
| reniform in u.s. cotton: when, where, why, and some remedies. | the reniform nematode, rotylenchulus reniformis, is an emerging problem in u.s. cotton. the impact of this nematode and the extent to which it has and will continue to spread across the u.s. cotton belt are controversial. long-term changes in cotton production and unique biological attributes of r. reniformis are key factors. expert opinion surveys indicate that r. reniformis has replaced the root-knot nematode (meloidogyne incognita) as the major nematode of cotton in mississippi, louisiana, an ... | 2007 | 17428179 |
| proteases from bacillus: a new insight into the mechanism of action for rhizobacterial suppression of nematode populations. | the aim of this study was to investigate the role of proteases in bacillus spp. of rhizobacteria in suppressing nematode populations and to understand their mechanism of action. | 2007 | 17718837 |
| physiological and genetic mapping study of tolerance to root-knot nematode in rice. | the root-knot nematode meloidogyne graminicola is an obligate biotrophic parasite and a major pest of rice (oryza sativa) for which resistant varieties are not currently available. quantitative trait loci (qtls) for partial resistance to m. graminicola were identified using a mapping population based on two rice varieties, bala x azucena. experiments were carried out to investigate the interactions between m. graminicola and these two varieties in terms of nematode establishment, reproduction an ... | 2007 | 17822410 |
| characterization of isolates of meloidogyne from rice-wheat production fields in nepal. | thirty-three isolates of root-knot nematode were recovered from soil samples from rice-wheat fields in nepal and maintained on rice cv. br 11. the isolates were characterized using morphology, host range and dna sequence analyses in order to ascertain their identity. results indicated phenotypic similarity (juvenile measurements, perennial pattern, host range and gall shape) of the nepalese isolates with meloidogyne graminicola, with minor variations. the rice varieties la 110 and labelle were s ... | 2007 | 19259491 |
| responses of heterodera glycines and meloidogyne incognita to exogenously applied neuromodulators. | biogenic amines regulate important behaviours in nematodes and are associated with pharyngeal activity in plant-parasitic nematodes. a robust behavioural assay based upon nematode body movements was developed to expand the study of these and other neuroregulators in plant-parasitic nematodes. dopamine, octopamine and serotonin each had significant but differing effects on the behaviour of soybean cyst nematode heterodera glycines and root-knot nematode meloidogyne incognita juveniles. body movem ... | 2007 | 18005465 |
| consumption rate of phytonematodes by pergalumna sp. (acari: oribatida: galumnidae) under laboratory conditions determined by a new method. | although several generalist species of pergalumna are known to be nematode predators, the potential of oribatid mites as natural enemies of phytonematodes has been underestimated. the objective of this work was to estimate the consumption rate of a pergalumna sp. when feeding on two major pest nematodes, meloidogyne javanica and pratylenchus coffeae, under laboratory conditions. a new method was used, in which live nematodes are offered to mites and subsequently consumption is quantified based o ... | 2007 | 17357821 |
| linkage maps of grapevine displaying the chromosomal locations of 420 microsatellite markers and 82 markers for r-gene candidates. | genetic maps functionally oriented towards disease resistance have been constructed in grapevine by analysing with a simultaneous maximum-likelihood estimation of linkage 502 markers including microsatellites and resistance gene analogs (rgas). mapping material consisted of two pseudo-testcrosses, 'chardonnay' x 'bianca' and 'cabernet sauvignon' x '20/3' where the seed parents were vitis vinifera genotypes and the male parents were vitis hybrids carrying resistance to mildew diseases. individual ... | 2007 | 17380315 |
| morphological and molecular characteristics of a new species of pasteuria parasitic on meloidogyne ardenensis. | a species of the hyper-parasitic bacterium pasteuria was isolated from the root-knot nematode meloidogyne ardenensis infecting the roots of ash (fraxinus excelsior). it is morphologically different from some other pasteuria pathogens of nematodes in that the spores lack a basal ring on the ventral side of the spore and have a unique clumping nature. transmission electron microscopy (tem) showed that the clumps of spores are not random aggregates but result from the disintegration of the suicide ... | 2007 | 17399736 |
| resistance to root-knot nematode in tomato roots expressing a nematicidal bacillus thuringiensis crystal protein. | our laboratory has demonstrated previously that bacillus thuringiensis (bt) crystal (cry) proteins present in the cry5 and cry6 subclades intoxicate free-living nematodes. in this study, we tested whether the expression of nematicidal cry6a in transgenic plants provided protection against plant-parasitic nematodes. as bacterial codon usage is incompatible with expression in plants, two different codon-modified cry6a genes were synthesized for expression in plants. one was designed by maintaining ... | 2007 | 17451491 |
| enhanced nematicidal activity of organic and inorganic ammonia-releasing amendments by azadirachta indica extracts. | the nematicidal activities of ammonium sulfate, chicken litter and chitin, alone or in combination with neem (azadirachta indica) extracts were tested against meloidogyne javanica. soil application of these amendments or the neem extracts alone did not reduce the root galling index of tomato plants or did so only slightly, but application of the amendments in combination with the neem extracts reduced root galling significantly. soil analysis indicated that the neem extract inhibited the nitrifi ... | 2007 | 19259469 |
| molecular characterization and temporal expression analyses indicate that the mic (meloidogyne induced cotton) gene family represents a novel group of root-specific defense-related genes in upland cotton (gossypium hirsutum l.). | the molecular events underlying the resistance of upland cotton (gossypium hirsutum l.) to the root-knot nematode (rkn) are largely unknown. in this report, we further characterize the previously identified mic3 gene including the identification of 14 related mic cdnas in nematode-infected roots of allotetraploid cotton that show >85% identity with mic3. a time-course analysis of rkn infection in resistant and susceptible cotton lines showed that maximum mic transcript accumulation occurred imme ... | 2008 | 18357465 |
| management of root knot nematode on tomato through application of fungal antagonists, acremonium strictum and trichoderma harzianum. | management of root knot nematode disease infecting tomato, by the use of fungal bioagents acremonium strictum and trichoderma harzianum isolated from egg masses of m. incognita infecting tomato has been carried out. the rhizosphere and rhizoplane of root knot nematode infested tomato revealed consistent association of acremonium strictum. in the present study a. strictum and other fungal bioagents viz. aspergillus niger, paecilomyces lilacinus, rhizoctonia solani and trichoderma harzianum isolat ... | 2008 | 18368544 |
| potential of olive mill waste and compost as biobased pesticides against weeds, fungi, and nematodes. | the phytotoxic and antimicrobial properties of olive mill wastes have been widely investigated and demonstrated over the past decade. however, their potential utilization as biodegradable pesticides against plant pathogens is still poorly understood. in this study, a series of laboratory bioassays was designed to test the inhibitory effects of sterile water extracts of two-phase olive mill waste (tpomw) and tpomw composts with different degrees of stabilization on several different plant pathoge ... | 2008 | 18471866 |
| laboratory trials to infect insects and nematodes by some acaropathogenic hirsutella strains (mycota: clavicipitaceous anamorphs). | laboratory assays have been carried out to artificially infect insect larvae of the birch bark-beetle (scolytus ratzeburgi jans.-coleoptera, scolytidae) and codling moth cydia pomonella l. -lepidoptera, tortricidae) as well as the potato cyst nematode-globodera rostochiensis wollenweber, sugar beet nematode-heterodera schachtii schmidt and root-knot nematode-meloidogyne hapla chif (nematoda, heteroderidae), by the phialoconidia of some fungal species of the genus hirsutella. from among four spec ... | 2008 | 17920621 |
| a plant growth promoting rhizobacterium, paenibacillus polymyxa strain gbr-1, suppresses root-knot nematode. | exposure of root-knot nematode, meloidogyne incognita to various concentrations (5-100%) of culture filtrate of paenibacillus polymyxa gbr-1 under in vitro conditions significantly reduced egg hatch and caused substantial mortality of its juveniles. the increase in the exposure durations of juveniles to culture filtrate and its concentrations increased the mortality rate. similarly, higher concentrations increased its inhibitory effect on egg hatch. in higher concentrations (25-100%) egg hatch w ... | 2008 | 17706411 |
| nematicidal prenylated flavanones from phyllanthus niruri. | two prenylated flavanones have been isolated from the hexane extract of phyllanthus niruri plant. the structure of these flavanones were established as 8-(3-methyl-but-2-enyl)-2-phenyl chroman-4-one (1) and 2-(4-hydroxyphenyl)-8-(3-methyl-but-2-enyl)-chroman-4-one (2) on the basis of spectral analysis. these were evaluated for nematicidal activity against root-knot, meloidogyne incognita, and reniform, rotylenchulus reniformis, nematodes. compound 2 exhibited nematicidal activity at par with the ... | 2008 | 17904597 |
| molecular detection of nematicidal crystalliferous bacillus thuringiensis strains of iran and evaluation of their toxicity on free-living and plant-parasitic nematodes. | the characterization of nematode-effective strains and cry genes in the iranian bacillus thuringiensis (bt) collection (70 isolates) is presented. characterization was based on pcr analysis using 12 specific primers for cry5, cry6, cry12, cry13, cry14, and cry21 genes encoding proteins active against nematodes, crystal morphology, and protein band patterns as well as their nematicidal activity on root-knot nematode (meloidogyne incognita) and two free-living nematodes (chiloplacus tenuis and acr ... | 2008 | 18923549 |
| distinct heat-shock element arrangements that mediate the heat shock, but not the late-embryogenesis induction of small heat-shock proteins, correlate with promoter activation in root-knot nematode feeding cells. | genes coding small heat-shock proteins (shsps) show distinct behaviours with respect to environmental and developmental signals. their transcriptional regulation depends on particular combinations of heat stress cis-elements (heat-shock elements; hses) but many aspects regarding their regulation remain unclear. cyst and root-knot nematodes induce, in the roots of infected plants, the differentiation of special feeding cells with high metabolic activity (syncytia and giant cells, respectively), a ... | 2008 | 18046507 |
| an easy method using glutaraldehyde-introduced fluorescence for the microscopic analysis of plant biotrophic interactions. | the method introduced in this article makes use of the glutaraldehyde-induced auto-fluorescence of proteins after cross-linking with glutaraldehyde for the analysis of cellular and sub-cellular structures. because the interface of biotrophic interactions is rich in proteins, the method presented is particularly suitable for the analysis of such interactions; we have exemplified its usefulness by analyzing (1) the root feeding sites induced in roots from arabidopsis thaliana by the root-knot nema ... | 2008 | 18778431 |
| characterization of root-knot nematode resistance in medicago truncatula. | root knot (meloidogyne spp.) and cyst (heterodera and globodera spp.) nematodes infect all important crop species, and the annual economic loss due to these pathogens exceeds $90 billion. we screened the worldwide accession collection with the root-knot nematodes meloidogyne incognita, m. arenaria and m. hapla, soybean cyst nematode (scn-heterodera glycines), sugar beet cyst nematode (sbcn-heterodera schachtii) and clover cyst nematode (clcn-heterodera trifolii), revealing resistant and suscepti ... | 2008 | 19259519 |
| detection and investigation of soil biological activity against meloidogyne incognita. | greenhouse experiments with two susceptible hosts of meloidogyne incognita, a dwarf tomato and wheat, led to the identification of a soil in which the root-knot nematode population was reduced 5- to 16-fold compared to identical but pasteurized soil two months after infestation with 280 m. incognita j2/100 cm(3) soil. this suppressive soil was subjected to various temperature, fumigation and dilution treatments, planted with tomato, and infested with 1,000 eggs of m. incognita/100 cm(3) soil. ei ... | 2008 | 19259527 |
| [degradation of soil phenolic acids by phanerochaete chrysosporium under continuous cropping of cucumber]. | with inoculation of phanerochaete chrysosporium in shaking flask, this paper studied the degradation of soil p-hydroxybenzoic acid, vanillic acid and ferulic acid under continuous cropping of cucumber, and evaluated the effect of this inoculation in overcoming the continuous cropping obstacle of cucumber. the results showed that after 8 days of inoculation, more than 99% of soil phenolic acids were decomposed by p. chrysosporium. compared with the control, the contents of soil phenolic acids und ... | 2008 | 19238850 |
| inter- and intra-specific cuticle variation between amphimictic and parthenogenetic species of root-knot nematode (meloidogyne spp.) as revealed by a bacterial parasite (pasteuria penetrans). | specific host-parasite interactions exist between species and strains of plant parasitic root-knot nematodes and the gram-positive bacterial hyperparasite pasteuria penetrans. this bacterium produces endospores that adhere to the cuticle of migrating juveniles, germinate and colonise the developing female within roots. endospore attachment of p. penetrans populations to second-stage juveniles of the root-knot nematode species meloidogyne incognita and meloidogyne hapla showed there were interact ... | 2008 | 18171577 |
| effect of application timing and method on efficacy and phytotoxicity of 1,3-d, chloropicrin and metam-sodium combinations in squash plasticulture. | metam-sodium, 1,3-dichloropropene (1,3-d) and chloropicrin are widely used soil fumigants. combined application of metam-sodium and 1,3-d + chloropicrin is intended to improve efficacy and broaden spectrum of control, but little is known about the effect on crop safety. this study aimed to evaluate the effects of application timing of fumigant combinations on soilborne pest and disease control (nematodes, soil fungi and weeds) and growth of squash. two separate tests with chisel-injected and dri ... | 2008 | 18181144 |
| new strategy for isolating novel nematicidal crystal protein genes from bacillus thuringiensis strain ybt-1518. | we have developed a strategy for isolating cry genes from bacillus thuringiensis. the key steps are the construction of a dna library in an acrystalliferous b. thuringiensis host strain and screening for the formation of crystal through optical microscopy observation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) analyses. by this method, three cry genes--cry55aa1, cry6aa2, and cry5ba2--were cloned from rice-shaped crystals, producing b. thuringiensis ybt-1518, which co ... | 2008 | 18820056 |
| potentiality of different isolates of wilt fungus fusarium oxysporum collected from rhizosphere of tomato against root-knot nematode meloidogyne incognita. | this investigation was undertaken to determine the effect of culture filtrates of different isolates of fusarium oxysporum f. sp. lycopersici on mortality of meloidogyne incognita juveniles and egg hatching. it was observed that different concentrations including standard extract (s.e), 1:10 and 1:100 dilutions of all fungal filtrates inhibited egg hatch when compared with control. minimum mortality and maximum hatching was observed in brt (showing least mortality) isolate of f. oxysporum, while ... | 2008 | 18941992 |
| infection by meloidogyne artiellia does not break down resistance to races 0, 1a, and 2 of fusarium oxysporum f. sp. ciceris in chickpea genotypes. | fusarium oxysporum f. sp. ciceris, and the root-knot nematode meloidogyne artiellia, coinfect chickpea crops in several countries of the mediterranean basin. the influence of root infection by m. artiellia on the reactions of chickpea genotypes with different reaction to infection with f. oxysporum f. sp. ciceris races 0, 1a, and 2 was investigated under controlled environmental conditions. results demonstrated that co-infection of chickpea genotypes resistant to specific fungal races by m. arti ... | 2008 | 18944296 |
| direct identification of the meloidogyne incognita secretome reveals proteins with host cell reprogramming potential. | the root knot nematode, meloidogyne incognita, is an obligate parasite that causes significant damage to a broad range of host plants. infection is associated with secretion of proteins surrounded by proliferating cells. many parasites are known to secrete effectors that interfere with plant innate immunity, enabling infection to occur; they can also release pathogen-associated molecular patterns (pamps, e.g., flagellin) that trigger basal immunity through the nematode stylet into the plant cell ... | 2008 | 18974830 |
| interactions between escherichia coli and the plant-parasitic nematode meloidogyne javanica. | to determine the potential of the plant-parasitic nematode meloidogyne javanica to serve as a temporary reservoir for escherichia coli. | 2008 | 19016981 |
| interaction of concurrent populations of meloidogyne partityla and mesocriconema xenoplax on pecan. | the effect of the interaction between meloidogyne partityla and mesocriconema xenoplax on nematode reproduction and vegetative growth of carya illinoinensis 'desirable' pecan was studied in field microplots. meloidogyne partityla suppressed reproduction of m. xenoplax, whereas the presence of m. xenoplax did not affect the population density of m. partityla second-stage juveniles in soil. above-ground tree growth, as measured by trunk diameter 32 months following inoculation, was reduced in the ... | 2008 | 19440263 |
| map65-3 microtubule-associated protein is essential for nematode-induced giant cell ontogenesis in arabidopsis. | the infection of plants by obligate parasitic nematodes constitutes an interesting model for investigating plant cytoskeleton functions. root knot nematodes have evolved the ability to manipulate host functions to their own advantage by redifferentiating root cells into multinucleate and hypertrophied feeding cells. these giant cells result from repeated rounds of karyokinesis without cell division. detailed functional analyses demonstrated that arabidopsis thaliana microtubule-associated protei ... | 2008 | 18263774 |
| ecological relationships between meloidogyne spartinae and salt marsh grasses in connecticut. | healthy specimens of selected grasses were collected from salt marshes and grown in the greenhouse. plants were inoculated with meloidogyne spartinae to determine the host range of this nematode. after 12 weeks, spartina alterniflora plants formed root galls in response to infection and increased m. spartinae populations. spartina patens, spartina cynosuroides, juncus gerardii and distichlis spicata were non-hosts. in order to determine the natural distribution of m. spartinae in dieback areas, ... | 2008 | 19440262 |
| molecular and morphological characterization of an unusual meloidogyne arenaria population from traveler's tree, ravenala madagascariensis. | an unusual variant of meloidogyne arenaria was discovered on roots of a traveler's tree (ravenala madagascariensis) intended for display at a public arboretum in pennsylvania. the population aroused curiosity by the lack of visible galling on the roots of the infected plant, and the female vulval region was typically surrounded by egg sacs. most morphometrics of the population fit within the ranges reported for m. arenaria, with a mosaic of features in common with either m. platani or other trop ... | 2008 | 19440257 |
| atcdka;1 silencing in arabidopsis thaliana reduces reproduction of sedentary plant-parasitic nematodes. | the activity of the arabidopsis thaliana cyclin-dependent kinase atcdka;1 is important throughout g(1)/s and g(2)/m transitions and guarantees the progression of the cell cycle. inhibitor studies have shown that activation of the cell cycle is important for the development of nematode feeding sites. the aim of this study was to silence the expression of the atcdka;1 gene in nematode feeding sites to interfere with their development. therefore, sense and antisense constructs were made for the atc ... | 2008 | 18554267 |
| histological characterization of root-knot nematode resistance in cowpea and its relation to reactive oxygen species modulation. | root-knot nematodes (meloidogyne spp.) are sedentary endoparasites with a broad host range which includes economically important crop species. cowpea (vigna unguiculata l. walp) is an important food and fodder legume grown in many regions where root-knot nematodes are a major problem in production fields. several sources of resistance to root-knot nematode have been identified in cowpea, including the widely used rk gene. as part of a study to elucidate the mechanism of rk-mediated resistance, t ... | 2008 | 18375605 |
| silencing a candidate nematode effector gene corresponding to the tomato resistance gene mi-1 leads to acquisition of virulence. | the mi-1 gene in tomato confers effective resistance against several species of root-knot nematode, including meloidogyne javanica. a strain of m. javanica that can reproduce on tomato with mi-1 was obtained from a culture of an avirulent strain after greenhouse selection. dna blots and amplified fragment length polymorphism (aflp) analysis indicated that the two nematode strains are closely related. expression patterns visualized as cdna aflps were nearly identical except for a cdna fragment, c ... | 2008 | 18393617 |
| detection and quantification of root-knot nematode (meloidogyne javanica), lesion nematode (pratylenchus zeae) and dagger nematode (xiphinema elongatum) parasites of sugarcane using real-time pcr. | a number of different plant parasitic nematode species are found associated with sugarcane in south africa. of these, the root-knot nematode (meloidogyne javanica), the lesion nematode (pratylenchus zeae) and the dagger nematode (xiphinema elongatum) are potentially the most damaging pests. identification and enumeration of the number of these nematodes are necessary for providing advice to farmers as well as studying the effects of various treatments in field and glasshouse trials. we report on ... | 2008 | 18378423 |
| alternate row placement is ineffective for cultural control of meloidogyne incognita in cotton. | the objective of this study was to determine if planting cotton into the space between the previous year's rows reduces crop loss due to meloidogyne incognita compared to planting in the same row every year. row placement had a significant (p </= 0.05) effect on nematode population levels only on 8 july 2005. plots receiving 1,3-dichloropropene plus aldicarb had lower nematode population levels than non-fumigated plots on 24 may and 8 july in 2005, but not in 2004. the effect of nematicide treat ... | 2008 | 19440259 |
| a transgressive segregation factor (rkn2) in gossypium barbadense for nematode resistance clusters with gene rkn1 in g. hirsutum. | host plant resistance is an important strategy for managing root-knot nematode (meloidogyne incognita) in cotton (gossypium l.). here we report evidence for enhanced resistance in interspecific crosses resulting from transgressive segregation of clustered gene loci. recently, a major gene, rkn1, on chromosome 11 for resistance to m. incognita in cv. acala nemx was identified using an intraspecific g. hirsutum cross with susceptible cv. acala sj-2. using interspecific crosses of acala nemxxsuscep ... | 2008 | 17940800 |
| host plant response of burrowing nematode resistant musa genotypes to the root-knot nematode, meloidogyne incognita. | 2008 | 18831278 | |
| pentacyclic triterpenoids from the aerial parts of lantana camara and their nematicidal activity. | two new olean-12-ene triterpenoids, camarolic acid (1) and lantrigloylic acid (2), have been isolated from the aerial parts of lantana camara, along with ten known triterpenes, namely, camaric acid, lantanolic acid, lantanilic acid, pomolic acid, camarinic acid, lantoic acid, camarin, lantacin, camarinin, and ursolic acid. the new compounds have been characterized as 3,25-epoxy-3alpha-hydroxy-22beta-{[(s)-3-hydroxy-2-methylidenebutanoyl]oxy}olean-12-en-28-oic acid (1) and 3,25-epoxy-3alpha-hydro ... | 2008 | 18816515 |
| biocontrol of root-knot nematode, meloidogyne incognita damaging queen palm, livistona rotundifolia using trichoderma species. | livistona rotundifolia is a widely grown queen palm in all the net houses of floriculture industries in sri lanka. it is grown to an extent of 10,000 mz in green farms ltd, marawilla under shade net house. root knot nematode is one among the key pests of queen palms and causes heavy loss in the queen palm industry. queen palm is grown in a coir based compost media under sprinkler irrigation. management of nematodes using chemicals is impractical due the non availability and selectivity of the ne ... | 2008 | 19226812 |
| dose-response effects of clove oil from syzygium aromaticum on the root-knot nematode meloidogyne incognita. | clove oil, derived from the plant syzygium aromaticum (l.) merr. & perry, is active against various organisms, and was prepared in a soy lecithin/detergent formulation to determine concentrations active against the root-knot nematode meloidogyne incognita (kofoid and white) chitwood. | 2008 | 18080287 |
| evaluation of the effect of ecologic on root knot nematode, meloidogyne incognita, and tomato plant, lycopersicon esculenum. | nonchemical methods and strategies for nematode management including cultural methods and engineered measures have been recommended as an alternative to methyl bromide (a major soil fumigant), due to its role in the depletion of the ozone layer. hence, an international agreement has recently been reached calling for its reduced consumption and complete phasing out. this present research evaluates the potential of ecologic, a biological, marine shell meal chitin material, as a soil amendment mana ... | 2008 | 18678924 |
| genome sequence of the metazoan plant-parasitic nematode meloidogyne incognita. | plant-parasitic nematodes are major agricultural pests worldwide and novel approaches to control them are sorely needed. we report the draft genome sequence of the root-knot nematode meloidogyne incognita, a biotrophic parasite of many crops, including tomato, cotton and coffee. most of the assembled sequence of this asexually reproducing nematode, totaling 86 mb, exists in pairs of homologous but divergent segments. this suggests that ancient allelic regions in m. incognita are evolving toward ... | 2008 | 18660804 |
| larvicidal and nematicidal activities of the leaf essential oil of croton regelianus. | the chemical composition of the leaf essential oil of croton regelianus collected from wild plants growing in two different sites at ceará state (brazil) was analyzed by gc/ms and gc-fid. twenty monoterpenoids, representing more than 96% of the chemical composition of the oils, were identified and quantified. the oils showed similar chemical composition but considerable variation in the levels of each constituent. ascaridole (33.9-17.0%), p-cymene (22.3-21.6%), and camphor (13.0-3.1%) were the p ... | 2008 | 19089831 |
| mining the secretome of the root-knot nematode meloidogyne chitwoodi for candidate parasitism genes. | parasite proteins secreted at the interface of nematode and host are believed to play an essential role in parasitism. here, we present an efficient pipeline of bio-informatic algorithms and laboratory experiments to identify candidate parasitism genes within nematode secretomes, i.e. the repertoire of secreted proteins in an organism. we performed our approach on 12 218 expressed sequence tags (ests) originating from three life stages of the plant parasitic nematode meloidogyne chitwoodi--a mol ... | 2008 | 18705879 |
| silip: a novel stable isotope labeling method for in planta quantitative proteomic analysis. | due to ease of manipulation, metabolic isotope coding of samples for proteomic analysis is typically performed in cell culture, thus preventing an accurate in vivo quantitative analysis, which is only achievable in intact organisms. to address this issue in plant biology, we developed silip (stable isotope labeling in planta) using tomato plants (solanum lycopersicum cv. rutgers) as a method that allows soil-grown plants to be efficiently labeled using a 14n/15n isotope coding strategy. after 2 ... | 2008 | 18665915 |
| genetic tests of ancient asexuality in root knot nematodes reveal recent hybrid origins. | the existence of "ancient asexuals", taxa that have persisted for long periods of evolutionary history without sexual recombination, is both controversial and important for our understanding of the evolution and maintenance of sexual reproduction. a lack of sex has consequences not only for the ecology of the asexual organism but also for its genome. several genetic signatures are predicted from long-term asexual (apomictic) reproduction including (i) large "allelic" sequence divergence (ii) lac ... | 2008 | 18606000 |
| root-knot nematodes manipulate plant cell functions during a compatible interaction. | sedentary endoparasitic nematodes are root parasites that interact with their hosts in a remarkable way. these obligate biotrophic pathogens establish an intimate relationship with their host plants, inducing the redifferentiation of root cells into specialized feeding cells. the successful establishment of feeding cells is essential for nematode development. root-knot nematodes, of the genus meloidogyne, have evolved strategies enabling them to induce feeding cell formation in thousands of plan ... | 2008 | 17681399 |
| cytoskeleton reorganization, a key process in root-knot nematode-induced giant cell ontogenesis. | root-knot nematodes are plant parasitic worms that establish and maintain an intimate relationship with their host plants. rkn induce the redifferentiation of root cells into multinucleate and hypertrophied giant cells essential for nematode growth and reproduction. major rearrangements of the cytoskeleton occur during giant cell formation. we characterized the first plant candidate genes implicated in giant cell actin and microtubule cytoskeleton reorganization. we showed previously that formin ... | 2008 | 19704567 |
| the role of different inoculum levels of meloidogyne javanica juveniles on nematode reproduction and host response of peanut plant. | a pot experiment was conducted to determine the influence of three of inoculum levels (1000, 2000 and 3000 j2 pot(-1)) of meloidogyne javanica on nematode reproduction and host response of peanut plant cv. giza 4 under greenhouse conditions at 30 +/- 5 degrees c. in general, nematode reproduction and host damage were both affected by the initial inoculum levels. the greater reduction percentage of plant fresh (57.7%), shoot dry (38.82) and pods weights (52.59%) and nodules numbers (73.33%) were ... | 2009 | 19580011 |
| [changes of soil biological characters beneath greenhouse cucumber under different cultivation systems]. | a 3-year pot experiment with 8-year continuously cropped greenhouse cucumber soil was conducted to study the effects of different cultivation systems on the soil quality, soil nematode quantity, and crop yields. the third year' s data were taken into analysis. comparing with traditional cultivation system (two seasons planting cucumber with fallowing in summer) , planting leafy vegetable and garlic in summer increased the yields of the two crops and the soil microbial biomass carbon content, tot ... | 2009 | 19565763 |
| the genomes of root-knot nematodes. | plant-parasitic nematodes are the most destructive group of plant pathogens worldwide and are extremely challenging to control. the recent completion of two root-knot nematode genomes opens the way for a comparative genomics approach to elucidate the success of these parasites. sequencing revealed that meloidogyne hapla, a diploid that reproduces by facultative, meiotic parthenogenesis, encodes approximately 14,200 genes in a compact, 54 mpb genome. indeed, this is the smallest metazoan genome c ... | 2009 | 19400640 |
| determination of preferred ph for root-knot nematode aggregation using pluronic f-127 gel. | root-knot nematodes (meloidogyne spp.) are obligate endoparasites of a wide range of plant species. the infective stage is attracted strongly to and enters host roots at the zone of elongation, but the compounds responsible for this attraction have not been identified. we developed a simple assay to investigate nematode response to chemical gradients that uses pluronic f-127, a synthetic block copolymer that, as a 23% aqueous solution, forms a liquid at low temperature and a gel at room temperat ... | 2009 | 19838866 |
| effects of the mi-1 and the n root-knot nematode-resistance gene on infection and reproduction of meloidogyne enterolobii on tomato and pepper cultivars. | meloidogyne enterolobii is widely considered to be an aggressive root-knot nematode species that is able to reproduce on root-knot nematode-resistant tomato and pepper cultivars. in greenhouse experiments, m. enterolobii isolates 1 and 2 from switzerland were able to reproduce on tomato cultivars carrying the mi-1 resistance gene as well as an n-carrying pepper cultivar. reproduction factors (rf) ranged between 12 and 109 depending on the plant cultivar, with m. enterolobii isolate 2 being more ... | 2009 | 22661786 |
| meloidogyne incognita: molecular cloning and characterization of a cdna encoding a cathepsin d-like aspartic proteinase. | herein we describe the cloning and characterization of a cdna encoding an aspartic proteinase from the root-knot nematode meloidogyne incognita. using pcr techniques, a 1471-bp cdna fragment encoding a cathepsin d-like (mi-asp1) transcript was isolated from second-stage larvae mrna. its predicted amino acid sequence comprises a pro-region of 71 amino acid residues and a mature protease of 378 amino acid residues with a predicted molecular mass of 41.502kda. protein sequence comparisons of mi-asp ... | 2009 | 18952081 |
| modular architecture and evolution of the map-1 gene family in the root-knot nematode meloidogyne incognita. | in eukaryotes, repeat proteins (i.e. proteins that contain a tandem arrangement of repeated structural elements) are often considered as an extra source of variability, and gains and losses of repeats may be an important force driving the evolution and diversification of such proteins, that could allow fast adaptation to new environments. here, we report genomic sequences of the map-1 protein family from of the asexual, plant-parasitic nematode meloidogyne incognita. the encoded proteins exhibit ... | 2009 | 19787376 |
| virulence development and genetic polymorphism in meloidogyne incognita (kofoid & white) chitwood after prolonged exposure to sublethal concentrations of nematicides and continuous growing of resistant tomato cultivars. | the root-knot nematode, meloidogyne incognita (kofoid & white) chitwood, is an important plant pathogen damaging to tomato. continuous use of resistant tomato cultivars and nematicides for its effective management might lead to resistance break-up or nematicide failure. genetic variability and virulence in m. incognita on susceptible pusa ruby tomato were analysed by bioassay, esterase and dna polymorphism after a 5 year weekly exposure to carbofuran, carbosulfan, cadusafos and triazophos at 0.0 ... | 2009 | 19562824 |
| distribution and identification of root-knot nematodes from turkey. | root knot nematodes are causing serious losses in protected cultivation fields in the west mediterranean region of turkey. correct and confident identification of the plant parasitic nematodes is important for vegetable growing and breeding. therefore, ninety-five populations of plant parasitic nematodes were collected from regional greenhouses. previously described species-specific primers were used to identify meloidogyne populations. the present study indicated that sec-1f/sec-1r and inck14f- ... | 2009 | 22661785 |
| cloning, sequence, and expression analysis of a new mnsod-encoding gene from the root-knot nematode meloidogyne incognita. | a gene encoding a manganese superoxide dismutase (mnsod) enzyme (mi-mnsod) was identified and characterized in second-stage juveniles of the root-knot nematode meloidogyne incognita. the mi-mnsod gene was found to possess five exons and four introns with (gt/ag) consensus splice-site junctions. the deduced amino acid sequence of mi-mnsod encodes a putative 25 kda protein, with conserved amino acid residues of the mnsod family, including the parker-blake signature and four metal-binding sites. th ... | 2009 | 22661777 |
| isolation of rna from laser-capture-microdissected giant cells at early differentiation stages suitable for differential transcriptome analysis. | plant organ gene expression profile analyses are complicated by the various cell types, and therefore transcription patterns, present in each organ. for example, each gall formed in roots following root knot nematode infection contains between four and eight specialized feeding cells (giant cells, gcs) embedded within hypertrophied root tissues. a recent goal in plant science has been the isolation of nematode feeding cell mrnas for subsequent gene expression analysis. by adapting current protoc ... | 2009 | 19523105 |
| reproduction of meloidogyne marylandi and m. incognita on several poaceae. | the susceptibility of 22 plant species to meloidogyne marylandi and m. incognita was examined in three greenhouse experiments. inoculum of m. marylandi was eggs from cultures maintained on zoysia matrella "cavalier" or cynodon dactylon x c. trasvaalensis "tifdwarf". inoculum of m. incognita was eggs from cultures maintained on solanum lycopersicum 'rutgers'. in each host test the inoculum density was 2,000 nematode eggs/pot. none of the three dicot species tested (gossypium hirsutum, arachis hyp ... | 2009 | 22661770 |
| reduction of root-knot nematode, meloidogyne javanica, and ozone mass transfer in soil treated with ozone. | ozone gas (o₃) is a reactive oxidizing agent with biocidal properties. because of the current phasing out of methyl bromide, investigations on the use of ozone gas as a soil-fumigant were conducted. ozone gas was produced at a concentration of 1% in air by a conventional electrical discharge o₃ generator. two o₃ dosages and three gas flow rates were tested on a sandy loam soil collected from a tomato field that had a resident population of root knot nematodes, meloidogyne javanica. at dosages eq ... | 2009 | 22736821 |
| meloidogyne polycephannulata n. sp. (nematoda: meloidogynidae), a root-knot nematode parasitizing carrot in brazil. | meloidogyne polycephannulata n. sp. is described from specimens collected from an area cultivated with carrot cv. brasilia, in the city of rio paranaíba, in the region of alto paranaíba, minas gerais state, brazil. the perineal pattern of the female is circular to ovoid with a high dorsal arch that has widely spaced, coarse annulations. the lateral field may have a deep furrow separating the dorsal and ventral arches. the medial lips are short and wide, whereas the lateral lips are large and tri ... | 2009 | 22736812 |
| population dynamics of meloidogyne arenaria and pasteuria penetrans in a long-term crop rotation study. | the endospore-forming bacterium pasteuria penetrans is an obligate parasite of root-knot nematodes (meloidogyne spp.). the primary objective of this study was to determine the effect of crop sequence on abundance of p. penetrans. the experiment was conducted from 2000 to 2008 at a field site naturally infested with both the bacterium and its host meloidogyne arenaria and included the following crop sequences: continuous peanut (arachis hypogaea) (p-p-p) and peanut rotated with either 2 years of ... | 2009 | 22736828 |
| assignment of aegilops variabilis eig chromosomes and translocations carrying resistance to nematodes in wheat. | the allotetraploid species aegilops variabilis eig (2n = 28, uusvsv) belongs to the tribe triticeae and is closely related to wheat. one accession, ae. variabilis no. 1, was found to be resistant to the cereal cyst nematode (ccn) and the root-knot nematode (rkn). as the genetic variability for resistance to those two pests is limited within wheat, this accession was crossed to bread wheat. previous work enabled the development of two addition lines and two translocation lines carrying resistance ... | 2009 | 19370089 |
| non-nematode-derived double-stranded rnas induce profound phenotypic changes in meloidogyne incognita and globodera pallida infective juveniles. | nine non-nematode-derived double-stranded rnas (dsrnas), designed for use as controls in rna interference (rnai) screens of neuropeptide targets, were found to induce aberrant phenotypes and an unexpected inhibitory effect on motility of root knot nematode meloidogyne incognita j2s following 24h soaks in 0.1 mg/ml dsrna; a simple soaking procedure which we have found to elicit profound knockdown of neuronal targets in globodera pallida j2s. we have established that this inhibitory phenomenon is ... | 2009 | 19482028 |
| using fame analysis to compare, differentiate, and identify multiple nematode species. | we have adapted the sherlock(®) microbial identification system for identification of plant parasitic nematodes based on their fatty acid profiles. fatty acid profiles of 12 separate plant parasitic nematode species have been determined using this system. additionally, separate profiles have been developed for rotylenchulus reniformis and meloidogyne incognita based on their host plant, four species and three races within the meloidogyne genus, and three life stages of heterodera glycines. stati ... | 2009 | 22736811 |
| european corn borer (lepidoptera: crambidae) stalk tunneling on root-knot nematode (tylenchida: heteroderidae) fitness on corn. | greenhouse experiments were conducted in 2004-2006 to examine the reciprocal effects of aboveground herbivory by european corn borer, ostrinia nubilalis (hübner) (lepidoptera: crambidae), and belowground herbivory by root-knot nematode, meloidogyne incognita chitwood (tylenchida: heteroderidae), on one another at three corn, zea mays l., growth stages. two experiments were conducted to study the effect of aboveground herbivory by o. nubilalis on the number of m. incognita juvenile penetration/ro ... | 2009 | 19449640 |
| phenotypic and molecular evaluation of cotton hairy roots as a model system for studying nematode resistance. | agrobacterium rhizogenes-induced cotton (gossypium hirsutum l.) hairy roots were evaluated as a model system for studying molecular cotton-nematode interactions. hairy root cultures were developed from the root-knot nematode (rkn) (meloidogyne incognita [kofoid and white] chitwood, race 3)-resistant breeding line m315 and from the reniform nematode (rn) (rotylenchulus reniformis linford & oliveira)-resistant accession gb713 (g. barbadense l.) and compared to a nematode-susceptible culture derive ... | 2009 | 19578854 |
| nematicidal efficacy of mcw-2, a new nematicide of the fluoroalkenyl group, against the root-knot nematode meloidogyne javanica. | the small number of available nematicides and restrictions on the use of non-fumigant nematicides owing to high toxicity to human and non-target organisms hinder effective nematode control. the nematicidal efficacy of mcw-2, a new nematicide of the fluoroalkenyl group, was evaluated against the root-knot nematode meloidogyne javanica (treub.) chitwood. | 2009 | 19488996 |
| a study of the changes in quantitative activity of some enzymes in olive cultivars during the interaction between verticillium wilt and root-knot nematode. | second stage juvenile (j2) of root-knot nematode, meloidogyne javanica, and microsclerotia of verticilliosis, verticillium dahliae, were used as the source of inoculum for nematode and fungus respectively. one-year-old seedlings of olive cultivar, zard, roghani, koroneiki and manzanilla, were transplanted to pots containing 2000g of sterilized sandy loam soil. experiment was conducted in completely randomized design with 32 treatments and five replications. treatments were as follows: control, n ... | 2009 | 20222618 |
| a study of interaction between verticillium wilt verticillium dahliae and root-knot nematode meloidogyne javanica in olive cultivars. | second stage juvenile (j2) of root-knot nematode, meloidogyne javanica, and microsclerotia of verticillium wilt, verticillium dahliae, were used as the source of inoculum for nematode and fungus respectively. one-year-old seedlings of olive cultivars, zard, roghani, koroneiki and manzanilla, were transplanted to pots containing 2000g of sterilized sandy loam soil. experiment was conducted in completely randomized design with 32 treatments and five replications. treatments were as follows: contro ... | 2009 | 20222619 |
| management of root-knot nematode, meloidogyne incognita in carrot. | the root-knot nematode, meloidogyne incognito, remains to be one of the most important constraints in agricultural production worldwide. however, reports showed that root-knot nematode (rkn) population can be suppressed by addition of organic amendments. a greenhouse microplot experiment was conducted to determine if locally available organic amendments would reduce rkn population and improve the growth and yield of more susceptible and less susceptible carrot cultivars in comparison with the fa ... | 2009 | 20222624 |
| evaluation of an antibiotic-producing strain of pseudomonas fluorescens for suppression of plant-parasitic nematodes. | the antibiotic 2,4-diacetylphloroglucinol (dapg), produced by some strains of pseudomonas spp., is involved in suppression of several fungal root pathogens as well as plant-parasitic nematodes. the primary objective of this study was to determine whether wood1r, a d-genotype strain of dapg-producing p. fluorescens, suppresses numbers of both sedentary and migratory plant-parasitic nematodes. an experiment was conducted in steam-heated soil and included two seed treatments (with wood1r and a cont ... | 2009 | 22736820 |
| actin-depolymerizing factor2-mediated actin dynamics are essential for root-knot nematode infection of arabidopsis. | reorganization of the actin and microtubule networks is known to occur in targeted vascular parenchymal root cells upon infection with the nematode meloidogyne incognita. here, we show that actin-depolymerizing factor (adf) is upregulated in the giant feeding cells of arabidopsis thaliana that develop upon nematode infection and that knockdown of a specific adf isotype inhibits nematode proliferation. analysis of the levels of transcript and the localization of seven adf genes shows that five ar ... | 2009 | 19794115 |
| differing requirements for flavonoids during the formation of lateral roots, nodules and root knot nematode galls in medicago truncatula. | * in this study, we tested whether the organogenesis of symbiotic root nodules, lateral roots and root galls induced by parasitic root knot nematodes (meloidogyne javanica) was regulated by the presence of flavonoids in the roots of medicago truncatula. flavonoids accumulate in all three types of root organ, and have been hypothesized previously to be required for secondary root organogenesis because of their potential role as auxin transport regulators. * using rna interference to silence the f ... | 2009 | 19402878 |
| plant growth-promoting rhizobacteria, paenibacillus polymyxa and paenibacillus lentimorbus suppress disease complex caused by root-knot nematode and fusarium wilt fungus. | to screen and evaluate the biocontrol potential of paenibacillus strains against disease complex caused by meloidogyne incognita and fusarium oxysporum f. sp. lycopersici interactions. | 2009 | 19457027 |
| tobacco rattle virus mediates gene silencing in a plant parasitic root-knot nematode. | root-knot nematodes (rkns) are sedentary biotrophic parasites that induce the differentiation of root cells into feeding cells that provide the nematodes with the nutrients necessary for their development. the development of new control methods against rkns relies greatly on the functional analysis of genes that are crucial for the development of the pathogen or the success of parasitism. in the absence of genetic transformation, rna interference (rnai) allows for phenotype analysis of nematode ... | 2009 | 19625337 |
| effect of a formulation of bacillus firmus on root-knot nematode meloidogyne incognita infestation and the growth of tomato plants in the greenhouse and nursery. | bacillus firmus, commercial wp formulation (bionem) was evaluated against the root-knot nematode meloidogyne incognita in a laboratory, greenhouse and under field conditions on tomato plants. in the laboratory tests, an aqueous suspension of bionem at 0.5%, 1%, 1.5% and 2% concentration reduced egg hatching from 98% to 100%, 24-days after treatment. treatment of second-stage juveniles with 2.5% and 3% concentration of bionem, caused 100% inhibition of mobility, 24 h after treatment. in the green ... | 2009 | 19041655 |
| functional characterization of transcripts expressed in early-stage meloidogyne javanica-induced giant cells isolated by laser microdissection. | the root-knot nematode meloidogyne javanica induces giant cells and feeds from them during its development and reproduction. to study the cellular processes underlying the formation of giant cells, laser microdissection was used to isolate the contents of early-stage giant cells 4 and 7 days post-infection (dpi) from tomato, and cdna libraries from both stages were generated with 87 [250 expressed sequence tag (est) clones] and 54 (309 est clones) individual transcripts identified, respectively. ... | 2009 | 19236572 |
| nematodetoxic aurovertin-type metabolites from a root-knot nematode parasitic fungus pochonia chlamydosporia. | chemical investigation of one fungal strain p. chlamydosporia ymf 1.00613 isolated from root knots of tobacco infected by meloidogyne incognita led to the isolation and identification of four aurovertin-type metabolites, which include a new compound, aurovertin i (a1), and three known metabolites, aurovertins e, f and d (a2-a4). their structures were established by spectroscopic studies such as 1d- and 2d-nmr and ms analysis. aurovertin i (a1) is the first natural product with an aurovertin skel ... | 2010 | 20000774 |
| early transcriptomic events in microdissected arabidopsis nematode-induced giant cells. | root-knot nematodes differentiate highly specialized feeding cells in roots (giant cells, gcs), through poorly characterized mechanisms that include extensive transcriptional changes. while global transcriptome analyses have used galls, which are complex root structures that include gcs and surrounding tissues, no global gene expression changes specific to gcs have been described. we report on the differential transcriptome of gcs versus root vascular cells, induced in arabidopsis by meloidogyne ... | 2010 | 20003167 |
| the b-3 ethylene response factor mterf1-1 mediates resistance to a subset of root pathogens in medicago truncatula without adversely affecting symbiosis with rhizobia. | the fungal necrotrophic pathogen rhizoctonia solani is a significant constraint to a range of crops as diverse as cereals, canola, and legumes. despite wide-ranging germplasm screens in many of these crops, no strong genetic resistance has been identified, suggesting that alternative strategies to improve resistance are required. in this study, we characterize moderate resistance to r. solani anastomosis group 8 identified in medicago truncatula. the activity of the ethylene- and jasmonate-respo ... | 2010 | 20713618 |
| identification of qtl in soybean underlying resistance to herbivory by japanese beetles (popillia japonica, newman). | soybean [glycine max (l.) merr.] was one of the most important legume crops in the world in 2010. japanese beetles (jb; popillia japonica, newman) in the us were an introduced and potentially damaging insect pest for soybean. jbs are likely to spread across the us if global warming occurs. resistance to jb in soybean was previously reported only in plant introductions. the aims here were to identify loci underlying resistance to jb herbivory in recombinant inbred lines (rils) derived from the cr ... | 2010 | 20458460 |
| management of root knot nematode, meloidogyne incognita in cucumber (cucumis sativus) using silicon. | silicon (si) has been reported to effectively manage some pests and diseases of plants. this study was conducted to determine the effect of si concentration, mode, and frequency of application in managing meloidogyne incognita in cucumber. a susceptible cultivar of cucumber (cv. cyclone) was planted in pots containing heat-sterilized soil. three weeks after planting, the plants were inoculated with 1,000 juveniles/ pot. uninoculated plants were provided to serve as control. three concentrations ... | 2010 | 21539270 |
| purification, characterization and molecular cloning of a monocot mannose-binding lectin from remusatia vivipara with nematicidal activity. | a mannose-binding lectin (rvl) was purified from the tubers of remusatia vivipara, a monocot plant by single-step affinity chromatography on asialofetuin-sepharose 4b. rvl agglutinated only rabbit erythrocytes and was inhibited by mucin, asialomucin, asialofetuin and thyroglobulin. lectin activity was stable up to 80 degrees c and under wide range of ph (2.0-9.3). sds-page and gel filtration results showed the lectin is a homotetramer of mr 49.5 kda, but maldi analysis showed two distinct peaks ... | 2010 | 20213246 |
| nematicidal activity of a nonpathogenic biocontrol bacterium, pseudomonas chlororaphis o6. | bacterial culture filtrates of an aggressive rhizobacterium, pseudomonas chlororaphis o6, displayed strong nematicidal activity. the nematicidal activity of p. chlororaphis o6 was markedly reduced in the gacs mutant of p. chlororaphis o6 grown in the presence of glycine, but no reduction of nematicidal activity in the gacs mutant was noted in the absence of glycine. the results of bioassay with p. chlororaphis o6 mutants showed that phenazine and pyrrolnitrin production was not a major factor, b ... | 2010 | 20963417 |
| persistence and nematicidal efficacy of carbosulfan, cadusafos, phorate, and triazophos in soil and uptake by chickpea and tomato crops under tropical conditions. | the productivity of chickpea, cicer arietinum (l.), and tomato, solanum lycopersicum (l.), is adversely affected by root-knot nematode, meloidogyne species. nematode-resistant chickpea and tomato are lacking except for a few varieties and therefore grower demand is not met. the available nematicides, namely, carbosulfan, cadusafos, phorate, and triazophos, were, therefore evaluated for their efficacy and persistence in soil and crops to devise nematode management decisions. in alluvial soil, cad ... | 2010 | 20085277 |
| proteomic and bioinformatic analysis of the root-knot nematode meloidogyne hapla: the basis for plant parasitism. | on the basis of the complete genome sequence of the root-knot nematode melodogyne hapla, we have deduced and annotated the entire proteome of this plant-parasite to create a database of 14,420 proteins. we have made this database, termed happep3, available from the superfamily repository of model organism proteomes (http://supfam.mrc-lmb.cam.ac.uk/superfamily). to experimentally confirm the happep3 assignments using proteomics, we applied a data-independent lc/ms(e) analysis to m. hapla protein ... | 2010 | 20804128 |
| short interfering rna-mediated gene silencing in globodera pallida and meloidogyne incognita infective stage juveniles. | the analysis of gene function through rna interference (rnai)-based reverse genetics in plant parasitic nematodes (ppns) remains inexplicably reliant on the use of long double-stranded rna (dsrna) silencing triggers; a practice inherently disadvantageous due to the introduction of superfluous dsrna sequence, increasing chances of aberrant or off-target gene silencing through interactions between nascent short interfering rnas (sirnas) and non-cognate mrna targets. recently, we have shown that no ... | 2010 | 19651131 |