Publications

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labial and vaginal microbiology: effects of extended panty liner use.objective: the goals of this study were 1) to better define the labial microflora and 2) to evaluate whether extended non-menstrual use of panty liners would increase genital carriage of undesirable bacteria and predispose to infection.methods: healthy female volunteers (224) were prospectively randomized into panty liner wear groups a (always((r)) deodorant) and b (always((r)) non-deodorant) and into a control group c (no panty liner wear) with instructions for non-menstrual +/- menstrual use > ...199718476146
immunoglobulin a response against gardnerella vaginalis hemolysin and sialidase activity in bacterial vaginosis.the aim of this study was to investigate the correlation between the immunoglobulin a immune response to gardnerella vaginalis hemolysin and sialidase activity in vaginal fluids from patients with bacterial vaginosis.19989539518
the role of gardnerella vaginalis in nonspecific vaginitis in intra uterine contraceptive device users.two hundred forty patients who had intra uterine contraceptive device (iucd) and manifested of nonspecific vaginitis were investigated for the presence of g. vaginalis. pure growth of this organism was obtained in 14(5.8%) cases while 116(48.3%) cases showed this organism in association with other organisms e.g. esch. coli (11.7%), klebsiella (9.2%), candida (9.2%), strept. faecalis (7.3%), proteus species (5.8%) and staph. albus (5%).19989581079
reiter's syndrome induced by gardnerella vaginalis. 19989751477
symptomatic bacteriuria due to ureaplasma and mycoplasma in adults.314 women, and 52 men were screened during 18 months, for the presence of ureaplasma urealyticum and mycoplasma hominis in a midstream specimens of urine. all the patients were adults with symptoms of urinary tract infection who consulted a physician at a primary health center "josé castro villagrana" in tlalpan, d.f. méxico. ureaplasma urealyticum was isolated in 54 (17.1%) of the women and 8 (15.4%) of the men. only escherichia coli and gardnerella vaginalis were isolated more frequently in bo ...199810932729
prevalence of gardnerella vaginalis in prepubertal males.to determine the rate of occurrence of gardnerella vaginalis in the genital tract and rectum of the asymptomatic prepubertal boy and to examine the effect of circumcision on the rate of recovery.19989811287
bacterial vaginosis: a review.bacterial vaginosis (bv) is a clinically definable condition that is frequently diagnosed in the primary care setting. changes in the nomenclature, causative microorganisms, as well as treatment have caused confusion over the last century. bv is currently viewed as a shift in the normal vaginal ecosystem from a predominance of aerobic bacteria to anaerobic bacteria (soper, 1993), resulting in a malodorous discharge. however, more than one half of clinically diagnosed patients are asymptomatic. r ...199810455564
[value of a computer-assisted screening method (papnet) for the detection of infectious cervico-uterine smears].there have been several reports regarding the accuracy of the papnet system applied to the screening for cancerous and precancerous lesions. based on neuronal networks, this computerized tool was initially trained to select atypical cells. it has been approved in the usa for the re-screening of cervical smears for quality assurance. however, its particular behaviour has not been frequently studied when the system is designed to detect frequent infectious organisms. we report the results of re-sc ...19989754374
phylogenetic analysis of the genus bifidobacterium and related genera based on 16s rdna sequences.the 16s rrna gene sequences were determined for type strains of 21 bifidobacterium species. a phylogenetic tree was constructed using the determined sequences and sequences from dna databases, which contain the sequences of 11 type strains of bifidobacterium species and 11 strains of related genera. all species of the genus bifidobacterium and gardnerella vaginalis atcc 14018 belonged to a cluster phylogenetically distinct from the other genera. the cluster was divided into two subclusters: subc ...19989858460
cervical cytology in women with bacterial vaginosis.398 symptomatic cases of vaginitis and 43 asymptomatic healthy women were subjected to cervical cytology and microbiological investigations for detection of bacterial vaginosis, g. vaginalis, t. vaginalis, c. albicans and m. hominis. cervical inflammatory changes were reported in 81.4% of cases and 60.4% of controls (p < 0.05) and were significantly associated with bacterial vaginosis and g. vaginalis infection (p < 0.01). cervical inflammation was detected in all cases of trichomonasis.19989805847
[prevalence of bacterial vaginosis in an obstetric population of barcelona].to investigate the prevalence and risk factors of bacterial vaginosis (bv) in a pregnant population of barcelona (spain).19989547730
[bacterial vaginosis: where are we?]. 19989547733
clinical findings in female genital schistosomiasis in madagascar.to assess the morbidity of s. haematobium infection in women of reproductive age (15-49 years) in the western part of madagascar, the village of betalatala with a prevalence of urinary schistosomiasis in women of 75.6% (95% confidence limit 69.3 to 81.9%) was compared with a neighbouring village with similar socio-economic characteristics and a prevalence of 5.0% (95% confidence limit 0 to 11.75%). the women were questioned in malagasy about obstetrical history and urogynecological symptoms. the ...19989623935
use of a continuous-culture biofilm system to study the antimicrobial susceptibilities of gardnerella vaginalis and lactobacillus acidophilus.gardnerella vaginalis and lactobacillus acidophilus have been shown to grow to high titers in a simple biofilm system. this system was used in the present investigation to compare the biofilm-eradicating concentrations (becs) of amoxicillin, clindamycin, erythromycin, and metronidazole to standard tube mic and minimum bactericidal concentration (mbc) results. with the lactobacillus, the bec/tube mbc ratio was at least 16:1, while for g. vaginalis the ratio varied from 2:1 to 512:1. the simple co ...19989624489
[the treatment of vaginal infections with macmiror and macmiror complex].the aim of the present study was to examine the therapeutic possibilities of the wide-spectrum medicament macmiror & macmiror complex for the treatment of the vaginal infections.19989859542
bacterial pathogens in male patients with urethritis in istanbul. 19989773441
impairment of the mucosal immune system: iga and igm cleavage detected in vaginal washings of a subgroup of patients with bacterial vaginosis.the integrity of the immunoglobulins in vaginal washings of patients with bacterial vaginosis was examined to answer the question of the lack of immune response against gardnerella vaginalis cytolysin. clinically diagnosed patients (n=100) were recruited and their vaginal washings examined by western blotting. many showed iga and igm partially or extensively degraded. according to the degradation pattern, the patients were subdivided into 4 subsets, from intact (score 0) to completely degraded i ...19989815222
relationship between maternal group b streptococcal colonization and pregnancy outcome.to examine the association of maternal colonization with group b streptococcus (gbs) with preterm labor and premature rupture of the membranes (prom).19989840551
can a laboratory diagnosis of bacterial vaginosis be made from a transported high vaginal swab using anaerobic culture and microscopy of a wet preparation? 19989849564
therapy of bacterial vaginosis. 19989511031
few microorganisms associated with bacterial vaginosis may constitute the pathologic core: a population-based microbiologic study among 3596 pregnant women.to evaluate the association between various microorganisms isolated from the genital tract in pregnant women with bacterial vaginosis.19989539529
chlamydia trachomatis: probable cause of prostatitis.seventy-eight men with symptoms of chronic or subacute prostatitis were enrolled. investigations for the presence of chlamydia trachomatis in urethral swabs were carried out. the expressed prostatic secretions were additionally examined for mycoplasma hominis, ureaplasma urealyticum, gardnerella vaginalis, other gram-negative and gram-positive bacteria, trichomonas vaginalis, yeast-like fungi and leucocyte count. furthermore, all patients were evaluated for the presence of serum anti-chlamydial ...19989671250
cytologic findings in a neovagina created with vecchietti's technique for treating vaginal aplasia.to report long-term cytologic findings after treatment of congenital vaginal aplasia or mayer-rokitansky-küster-hauser syndrome (mrkh) using progressive traction of the vulvar tissues, or vecchietti's technique (vt).19989684582
female genital tract bacterial coisolates with candida albicans in patients without clinical vaginitis.in vitro, candida albicans has demonstrated the ability to inhibit replication of selected bacteria. little information exists on the impact of c. albicans on the vaginal bacterial flora in vivo. the purpose of this study is to identify the coexisting bacterial flora when c. albicans is isolated from vaginal cultures submitted to a hospital-based testing facility for reasons other than vulvovaginitis.19989702585
preterm birth: associations with genital and possibly oral microflora.opportunistic pathogenic microbes are indigenous to the female lower genital tract and etiologic in many types of pelvic infections and, apparently, a portion of preterm birth (ptb) cases. bacterial vaginosis (bv) is a clinical syndrome based on an altered genital microflora in which gardnerella vaginalis; anaerobic species primarily among prevotella, porphyromonas, bacteroides, peptostreptococcus, and mobiluncus; mycoplasma hominis; and ureaplasma urealyticum become predominant in vaginal secre ...19989722706
characterization of actinomyces turicensis and actinomyces radingae strains from human clinical samples.whole-organism protein electrophoresis was used to compare and group unidentified coryneform bacteria resembling gardnerella vaginalis and various actinomyces and arcanobacterium species. the obtained clusters of strains were further characterized by whole-cell fatty acid analysis and a variety of biochemical tests. species-specific oligonucleotide probes based on 16s rrna gene sequences were designed. the results demonstrate that the majority of the isolates belonged to actinomyces turicensis; ...19989731291
bacterial vaginosis: association with adverse pregnancy outcome.bacterial vaginosis is the most common lower genital tract infection encountered among women of reproductive age. this condition can best be considered as a vaginal syndrome associated with an alteration of the normal vaginal flora rather than an infection specific to any one microorganism. bacterial vaginosis is a clinical condition with a complex microbiology that is characterized by a reduced concentration of a normally abundant lactobacillus species along with high concentrations of gram-neg ...19989738988
acquisition of iron by gardnerella vaginalis.six gardnerella vaginalis strains were examined for the ability to utilize various iron-containing compounds as iron sources. in a plate bioassay, all six strains acquired iron from ferrous chloride, ferric chloride, ferrous sulfate, ferric ammonium citrate, ferrous ammonium sulfate, bovine and equine hemin, bovine catalase, and equine, bovine, rabbit, and human hemoglobin. all six strains also acquired iron from human lactoferrin, but not from human transferrin, as determined by a liquid broth ...19989746616
the growth of gardnerella vaginalis and lactobacillus acidophilus in sorbarod biofilms.sorbarod biofilms were investigated for their suitability in establishing continuous culture biofilms for the study of bacterial vaginosis. two important organisms in the condition, gardnerella vaginalis and lactobacillus acidophilus, were studied. in contrast to growth in broth culture, both organisms were maintained for at least 96 h in a steady state on the biofilms. with g. vaginalis, the haemolytic activity was consistently maintained in the biofilms in contrast to short-term activity in br ...19989879940
gram stain method shows better sensitivity than clinical criteria for detection of bacterial vaginosis in surveillance of pregnant, low-income women in a clinical setting.the purpose of the study is to determine whether the gram stain method is superior to the clinical criteria for the diagnosis of bacterial vaginosis in low-income pregnant women seen in a resident clinic setting. the clinical criteria is the current diagnostic method employed to diagnose bacterial vaginosis.19989894174
[survey of sexually transmitted diseases in the region of rio cuarto].sexually transmitted diseases (std) are acquired mainly through sexual intercourse, being one of the most frequent groups of infectious diseases worldwide and consequently an important public health problem. the aim of this paper was to determine the current state of std and to compare different diagnostic methods in the population studied. a total of 1060 samples from vaginal flows, endocervical material and urethral discharge were studied during 3 years. of the total samples, 583 were positive ...19989922478
assessment of office-based care of sexually transmitted diseases and vaginitis and antibiotic decision-making by obstetrician-gynecologists.survey office-based obstetric-gynecologic practitioners regarding their knowledge of infectious disease care and antibiotic use.19989972486
risk factors for laparoscopically confirmed pelvic inflammatory disease: findings from mumbai (bombay), india.sexually transmitted diseases (stds) are an important cause of pelvic inflammatory disease (pid) but have often not been detected in microbiological studies of indian women admitted to hospital gynaecology wards or private clinics. in this cross sectional study, women living in the inner city of mumbai (bombay) were investigated for socioeconomic, clinical, and microbiological risk factors for pid.199810195052
recurrent bacterial vaginosis and metronidazole resistance in gardnerella vaginalis. 199810195061
clinical and microbiological aspects of vaginitis. 199810218298
in vitro activities of pazufloxacin, a novel injectable quinolone, against bacteria causing infections in obstetric and gynecological patients.t-3762 is an injectable new quinolone with a broad spectrum of antibacterial activity. pazufloxacin (t-3761) is an active form of t-3762. the minimal inhibitory concentrations (mics) of pazufloxacin for 50% of the clinical isolates tested were 3.13 microg/ml for streptococcus agalactiae, 6.25 microg/ml for gardnerella vaginalis, 0.025 microg/ml for escherichia coli, 0.78 microg/ml for pseudomonas aeruginosa, 6.25 microg/ml for peptostreptococcus magnus, 6.25 microg/ml for bacteroides fragilis an ...199910224336
the effects of three nonoxynol-9 preparations on vaginal flora and epithelium.to evaluate the effects of nonoxynol-9 (n-9) on the vaginal flora and epithelium, 48 women (16 in each group) were evaluated by use of quantitative vaginal cultures and colposcopy. at baseline and at 0.5, 4, 24, 48, and 72 h after insertion of one of three n-9 preparations (4% gel [conceptrol], 3.5% gel [advantage-24], or a 28% vaginal contraceptive film). the proportion positive for h2o2+ or h2o2- lactobacilli did not change significantly with any of the preparations, but lactobacilli concentra ...199910395859
bacterial vaginosis-associated microflora isolated from the female genital tract activates hiv-1 expression.alteration of cervicovaginal microbial flora can lead to vaginosis, which is associated with an increased risk of hiv-1 transmission. we recently characterized a soluble hiv-inducing factor (hif) from the cervicovaginal lavage (cvl) samples of women. the goals of this study were to determine the effect of cervicovaginal microflora on hiv-1 expression and to elucidate the relationship between hif activity and microflora. physiologically relevant microorganisms, mycoplasma, diphtheroid-like bacter ...199910421242
[chronic bacterial prostatitis]. 199910067117
activation of human immunodeficiency virus type 1 expression by gardnerella vaginalis.bacterial vaginosis (bv) is associated with an increased rate of sexual transmission of human immunodeficiency virus (hiv) type 1, and gardnerella vaginalis is frequently isolated from the genital tracts of women with bv. g. vaginalis lysates were found to significantly stimulate hiv expression in monocytoid cells. stimulation was significantly higher when lysates were heated at 100 degrees c for 5 min but was reduced by treatment with lysozyme or protease. g. vaginalis lysates also activated hi ...199910068588
acid production by vaginal flora in vitro is consistent with the rate and extent of vaginal acidification.perinatally, and between menarche and menopause, increased levels of estrogen cause large amounts of glycogen to be deposited in the vaginal epithelium. during these times, the anaerobic metabolism of the glycogen, by the epithelial cells themselves and/or by vaginal flora, causes the vagina to become acidic (ph approximately 4). this study was designed to test whether the characteristics of acid production by vaginal flora in vitro can account for vaginal acidity. eight vaginal lactobacillus is ...199910496892
association of indicators of bacterial vaginosis with a female genital tract factor that induces expression of hiv-1.the aim of this study was to determine the relationship of bacterial vaginosis and bacterial vaginosis-associated microorganisms with an hiv-inducing factor (hif) found in cervicovaginal lavage.199910513649
gram stain method shows better sensitivity than clinical criteria for detection of bacterial vaginosis in surveillance of pregnant, low-income women in a clinical setting. 199910598915
role of bacterial typing in genitourinary medicine. 199910615302
[a biotype study of gardnerella vaginalis isolated from patients with and without symptoms of bacterial vaginosis].a simple and reproducible proposed for benito et al. scheme for identification biotypes of gardnerella vaginalis has been developed, based on reactions for lipase, hippurate, hydrolysis, and beta-galactosidase.199910650646
bacterial vaginosis a "broad overview".bacterial vaginosis (bv) was first reported in 1995 by gardner and dukes, who described the unique clinical signs and symptoms and the distinctive nature of the vaginal discharge associated with it. they also described a "new" causative organism, which they named "haemophilus vaginalis", subsequently renamed gardnerella vaginalis. bv is currently the most prevalent cause of infectious vaginitis among women attending for genitourinary diseases. bv has a complex microbiology. lactobacillus populat ...199910932748
[verification of identification of infectious agents by culture in routine gynecologic cytological screening].the original bethesda classification system for reporting cervical/vaginal cytologic diagnoses has claimed besides oncologic evaluation also a statement on the presence of infectious agents. their diagnosis should be followed by appropriate treatment. based upon the comparison of careful bacterioscopic study in a series of 175 routine cervical smears with the results of microbiological, virological and mycological examinations the following pathogens might be-according to the authors' opinion-di ...199911038666
[a multicenter study of the antimicrobial effect of macmiror and macmiror complex in the treatment of vaginal infections].the aim of the present multicentre study was to examine the therapeutic possibilities of the wide-spectrum medicament macmiror & macmiror complex for the treatment of the vaginal infections.199910734688
[the antagonistic activity of bifidobacteria in vitro and in vivo studied by using gnotobiological technology].the antagonistic activity of 4 strains of bifidobacteria (b. adolescentis 2 f1, b. longum z4, b. breve r2 and b. bifidum g1), isolated from the vagina of healthy females of the reproductive age, with respect to escherichia coli, klebsiella ozaenae, staphylococcus aureus, enterococcus faecalis, pseudomonas aeruginosa and gardnerella vaginalis were studied in vitro and in vivo. the in vitro experiments revealed that all above-mentioned bifidobacteria were capable of inhibiting the growth of all in ...199910852027
[the range of antagonistic effects of lactobacillus bacterial strains on etiologic agents of bacterial vaginosis].bacterial vaginosis is caused by uncontrolled sequential overgrowth of some anaerobic bacteria: gardnerella vaginalis, prevotella bivia, bacteroides spp., peptostreptococcus spp., mobiluncus sp. usually occurring in stable numbers in the bacterial flora of healthy women. on the other hand, different species of bacteria belonging to the genus lactobacillus, most frequently l. plantarum, l. rhamnosus and l. acidophilus, form a group of aerobic bacteria dominating in the same environment. the diver ...199910865430
diagnostic methods for bacterial vaginosis. 199910661732
electron microscopy studies on gardnerella vaginalis grown in conventional and biofilm systems.the cell-wall characteristics of gardnerella vaginalis grown in conventional and biofilm systems were studied by electron microscopy. the gram-positive nature of the cell wall was confirmed. novel cell-wall particles which appeared to be associated with cell division were also identified, particularly in organisms of biofilm origin.19999989650
intravaginal bacterial flora in patients with uterine cervical cancer. high incidence of detection of gardnerella vaginalis.gynecological diseases may affect the growth of vaginal bacterial flora. we investigated the relationship between uterine cervical cancer and the vaginal bacterial flora. in 20 patients with uterine cervical cancer, we investigated the intravaginal bacterial flora, including gardnerella vaginalis and mobiluncus spp. in the patients with uterine cervical cancer, there was a mixed flora of aerobes and anaerobes and, of special note, g. vaginalis was detected in 50% of the patients with uterine cer ...199911810495
detection of gardnerella vaginalis in the vagina and amniotic fluid using the polymerase chain reaction. 200011102607
modern methods for diagnosis of gardnerella infection.comparison of morphological, bacteriological, serological, and genetic methods for diagnosis of gardnerella infection in vaginosis showed that the first three methods are preferable, while the genetic method (polymerase chain reaction) is more efficient for identification of gardnerella vaginalis in culture.200011177243
association of mycoplasma hominis and ureaplasma urealyticum with some indicators of nonspecific vaginitis.the purpose of this study was to determine the isolation rates of mycoplasma hominis and ureaplasma urealyticum from three populations of women and also to relate the presence of these microorganisms with some indicators of nonspecific vaginitis. three hundred vaginal swabs were taken from delivery, pregnant and control (not pregnant) women. cultures were done in e broth supplemented with arginine or urea. m. hominis was isolated in 5% at delivery, 12% from pregnant and 5% from control women and ...200010948822
inhibition of chemotaxis by organic acids from anaerobes may prevent a purulent response in bacterial vaginosis.it has been postulated that certain organic acids produced by the anaerobes associated with bacterial vaginosis (bv) could prevent a purulent response in this infection. varying concentrations of pure succinic, acetic and lactic acids were incubated in vitro with a monocytic cell line (monomac 6). high inhibition of chemotaxis was produced by succinic acid; lower inhibition and no inhibition was shown by acetic acid and lactic acid respectively. succinic and acetic acids were detected in high co ...200011073156
diagnosis of bacterial vaginosis by wet mount identification of bacterial morphotypes in vaginal fluid.in order to develop a more practical way of diagnosing bacterial vaginosis (bv), we evaluated a scoring system, weighting small bacterial morphotypes versus lactobacillary morphotypes in wet mounts, assessed criteria for bv and normalcy from this scoring, and then evaluated their reproducibility and accuracy. we examined 754 women for ph, homogeneous vaginal discharge, amine odour, clue cells and the composite clinical diagnosis. we also examined wet mounts for small bacterial morphotypes and la ...200010726936
[complications of sexually transmitted diseases: clinical course and treatment].sexually transmitted infectious agents cause a wide array of complications in the short-, medium-, and long-term, especially among women. specifically, infection with these agents can result in sterility, infertility, genital neoplasia, pre-term delivery, and foetal/neonatal pathologies, and the agents most commonly associated with these complications are: neisseria gonorrhoeae, chlamydia trachomatis, anaerobic bacteria, ureaplasma urealyticum, gardnerella vaginalis and human papilloma virus (hp ...200011367920
[the production of a latex immunoglobulin conjugate for the diagnosis of gardnerella vaginalis].to obtain an anti-gadnerella vaginalis latex globulin, specific immunosera to reference strain (atcc 14018) and to a clinical isolation strain pool were produced. these immunosera were characterized using page-sds and immunoblotting where a close antigenic relation between the clinical isolation strains and the reference strain was observed. the latex globulin conjugates obtained from these 2 immunosera were evaluated in vitro with a resulting level of detection of 10 ufc/ml of gardnerella vagin ...200011107902
[maternal-fetal infection with gardnerella vaginalis]. 200011109955
adverse obstetric sequelae of bacterial vaginosis.bacterial vaginosis is increasingly associated with adverse sequelae in obstetrics such as preterm prelabour rupture of membranes, preterm labour and preterm birth. it is important to diagnose the condition early in pregnancy where appropriate treatment can be administered to women who are symptomatic or who are at high risk of adverse sequelae.200011091802
characterization of the 16s rrna gene v2 region and the rrn operons of gardnerella vaginalis.ribosomal rna (rrna) gene polymorphism was apparent when gardnerella vaginalis dna restriction profiles were hybridized with nonradioactively labeled total rrna isolated from this bacterium. in contrast, use of a polymerase chain reaction (pcr)-based 16s rrna gene v2 region resulted in a 118-bp v2-pcr amplicon that was specific and common in all 30 tested g. vaginalis isolates. in addition to providing a g. vaginalis-specific fingerprint, when the v2-pcr amplicon along with total rrna were utili ...200011130865
a study on the possible association of dysfunctional uterine bleeding with bacterial vaginosis, mycoplasma, ureaplasma, and gardnerella vaginalis. 200011141864
clarithromycin-induced alterations in vaginal flora.many antibiotics have been shown to alter both the bacterial and the fungal flora of the vagina, in some cases potentially increasing a woman's propensity toward urinary tract infections and vaginal candidiasis. the effects of some of the newer macrolide antibiotics on women's vaginal flora have not been previously studied, and almost none of the previous studies specifically cultured for effects on vaginal lactobacillus. young women (ages 18-45 years) who were about to go onto therapy with clar ...200011317173
vaginal microflora in healthy women with gardnerella vaginalis.in order to find the vaginal prevalence of gardnerella vaginalis in a normal female population, we determined the incidence of g. vaginalis in relation to that of other bacterial genera and species in the vagina. two-hundred and thirty-nine healthy women were the subjects of this study. vaginal discharge was collected and bacteriological studies were performed. the mean total aerobe count in the g. vaginalis-positive group was 9.02 log10 colony-forming units (cfu)/g, which was significantly high ...200011810560
incidence of gardnerella vaginalis, candida sp and human papilloma virus in cytological smears.in spite of the wide-ranging literature on the microbiology of normal and abnormal flora of the vagina, there are few studies on the relationship between human papilloma virus (hpv) and other vaginal microorganisms.200010887386
[isolation of gardnerella vaginalis in the diagnosis of sexual abuse in children].the study of sexual transmitted diseases is more and more frequent in patient with suspicion of sexual abuse, and this help to the final medical diagnosis. organisms like neisseria gonorrhoeae and treponema indicate a sure sexual contact. however the role of the rest of organisms in the sexual abuse is more difficult of specifying.200010888024
[bacterial vaginosis. epidemiology and risk factors].bacterial vaginosis (bv) is a change in vaginal ecosystem where lactobacilli dominate, flora is absent or greatly reduced, and replaced with a mixed, predominantly anaerobic flora, consisting of gardnerella vaginalis, mycoplasma hominis, mobiluncus spp, bacteroides spp, prevotela spp, peptostreptococcus spp, fusobacterium spp and porphyromonas spp. the concentration of bacteria increases from 100 to 1000 fold in women with bv compared to healthy women. bv has been formerly known as nonspecific v ...200010916461
pathogenesis of abnormal vaginal bacterial flora.this study was undertaken to determine the relationships between microscopy findings on wet mounts, such as lactobacillary grade or vaginal leukocytosis, and results of vaginal culture, lactate and succinate content of the vagina, and levels of selected cytokines.200010764465
bacterial vaginosis.bacterial vaginosis represents a unique upheaval of the complex vaginal bacterial flora with disappearance of lactobacilli and overgrowth of gardnerella vaginalis and resident anaerobic vaginal bacteria. little progress has occurred in identifying causal factors, although the pathophysiology of this syndrome is better understood. although symptoms are easily recognizable, obstetric and gynecologic complications continue to increase in number. bacterial vaginosis is far more than a nuisance infec ...200010774469
in vitro activity of a cellulose acetate phthalate topical cream against organisms associated with bacterial vaginosis. 200010797102
identification of a human lactoferrin-binding protein in gardnerella vaginalis.previous studies have shown that gardnerella vaginalis can utilize iron-loaded human lactoferrin as a sole source of iron. in this study, g. vaginalis cells were shown to bind digoxigenin (dig)-labeled human lactoferrin in a dot blot assay. using the dig-labeled human lactoferrin, a 120-kda human lactoferrin-binding protein was detected by western blot analysis of g. vaginalis proteins. the lactoferrin-binding activity of this protein was found to be heat stable. competition studies indicated th ...200010816496
characterisation and selection of a lactobacillus species to re-colonise the vagina of women with recurrent bacterial vaginosis.this paper reports the results of characterising and selecting a strain of lactobacillus for potential use as a probiotic in regenerating the vaginal flora of women with recurrent episodes of bacterial vaginosis (bv). bv is a condition characterised by a depletion of vaginal lactobacilli accompanied by an overgrowth of a mixed vaginal flora of aerobic, anaerobic and micro-aerophilic species in very large numbers. bv has been associated with various gynaecological and obstetric complications and ...200010847208
conservation in probiotic preparations of lactobacillus with inhibitory capacity on other species.strains of lactobacillus isolated from dairy products and genital tract competed with candida albicans through a membrane of 12000 dalton cut-off. this inhibition was due to hydrogen peroxide and was trypsin-stable, heat-sensitive and antagonized by catalase. lactobacillus coming from "starters" showed antimicrobial activity against fungus isolated in a yogurt factory. penicillium, alternaria, phialophora, microsporum and candida spp. were inhibited when 10(2) spores were inoculated in the assay ...200011061631
[the treatment of bacterial vaginosis with dalacin v cream].to asses the efficiency of the 2% clindamycin phosphate creme given in 3 and 7 daily courses officially designer for the treatment of bacterial vaginosis (bv).200010948619
role of the male partner in the lower genitourinary tract infection of female.we studied the relationships of selected microbes and the role of consorts in the causation of vaginal discharge which may be due to cervicitis or vaginitis. a total of 93 consecutive patients in the reproductive age group with symptoms of vaginal discharge along with their sexual partners were studied. samples were collected from the cervix and posterior fornix of the female patients and from the urethra and sub-prepucial area of the male partner for culture of neisseria gonorrhoeae, gardnerell ...200011006655
bacterial vaginosis.bacterial vaginosis is the most common cause of vaginitis, affecting over 3 million women in the united states annually. depopulation of lactobacilli from the normal vaginal flora and overgrowth of gardnerella vaginalis and other anaerobic species are the presumed etiology. to date, no scientific evidence shows that bacterial vaginosis is a sexually transmitted disease. malodorous vaginal discharge is the most common symptom. differential diagnoses include trichomoniasis, moniliasis, and allergi ...200011025268
binding of catalase by gardnerella vaginalis.previous work has demonstrated that gardnerella vaginalis can utilize catalase as a sole source of iron. in this study, the interaction between g. vaginalis cells and catalase was investigated. g. vaginalis cells were shown to bind digoxigenin (dig)-labeled catalase using a solid phase dot blot assay. an increase in catalase binding was observed from cells grown under iron-restrictive conditions. western blot analysis of g. vaginalis proteins resulted in the detection of a putative catalase-bind ...200011034278
suitability of a unique 16s rrna gene pcr product as an indicator of gardnerella vaginalis. 200010683728
the diagnosis of bacterial vaginosis and vaginal flora changes.in a population of 956 women, attending for contraceptive advice, 131 (13.7%) were found to have bv acording to amsel's criteria. clue cells were detected in 200 (20.9%) women, a positive amine ('sniff') test in 191 (20.0%), a vaginal ph > or = 4.7 in 243 (25.4%) and a "characteristic" vaginal discharge in 104 (10.9%) women. sensitivity, specificity, positive and negative predictive values in relation to bv were calculated for each of these four criteria. the detection of clue cells, an increase ...200111327086
understanding the bacterial flora of the female genital tract.the microbiological flora of the lower female genital tract provides a dynamic, complex example of microbial colonization, the regulation of which is not fully understood. when an exogenous bacterial species, with its array of virulence factors, is introduced into the host, disease does not always occur. conversely, under selected conditions, commensal endogenous bacteria-for example, gardnerella vaginalis and group b streptococci-can participate in disease processes. disease caused by both exog ...200111181139
detection of cervical immunoglobulin a in normal pregnancy.the aim of our study was to evaluate a feasible method to quantify the immunoglobulin a concentration in the cervical mucus of women with a normal singleton pregnancy. in 60 immunologic healthy pregnant women cervical mucus samples were taken at a random time in pregnancy using an absorbing cylindrical cotton-swab stick. in this cervical mucus immunoglobulin a concentration was measured by radial immunodiffusion. a vaginal bacterial swab was taken from each woman. concentration of immunoglobulin ...200111234615
aerobic microbiological study in term pregnant women with premature rupture of the membranes: a case-control study.to determine the aerobic microorganisms related to premature rupture of the membranes (prom) in term pregnant women, a case-controlled study was performed on pregnant women delivered at rajavithi hospital between november 1, 1996 and july 30, 1997. two hundred and twenty pregnant women with prom and 220 pregnant women without prom were recruited by simple random sampling. the diagnosis of rupture of the membrane was made by history and by positive microscopic ferning and ph testing performed dur ...200111281495
predictors of the vaginal microflora.our purpose was to define influences on the patterns of the vaginal microflora.200111303191
binding of heme by gardnerella vaginalis.it has been previously demonstrated that gardnerella vaginalis could acquire iron from a number of different iron-containing compounds, including heme. in this study, the direct binding of heme by g. vaginalis strains was demonstrated utilizing a liquid broth heme-binding assay. competition studies demonstrated that pretreatment of g. vaginalis cells with other iron sources such as hemoglobin, catalase, and lactoferrin did not affect heme binding. also, heme binding was not inhibited by preincub ...200111314245
[frequency of gardnerella vaginalis vaginosis and its association with other pathogens causing genital infections in the female].in order to determine the presence of unspecific vaginosis and their causes, 700 vaginal smears were obtained from patients assisting to the clinical laboratory of the familiar medicine unit no. 28 "gabriel mancera" of the instituto mexicano del seguro social, during 6 months. the patients age was from 18 to 55 years old. to establish the infectious etiology of these pathologies the vaginal smears were observed freshly and stained by gram's method. specific test for differentiate gardnerella vag ...200111588810
new approach to phylogenetic analysis of the genus bifidobacterium based on partial hsp60 gene sequences.the partial 60 kda heat-shock protein (hsp60) genes of 36 bifidobacterium strains representing 30 different bifidobacterium species and subspecies and of the type strain of gardnerella vaginalis were cloned and sequenced using a pair of universal degenerate hsp60 pcr primers. the hsp60 dna sequence similarities were determined for the taxa at various ranks as follows: 99.4-100% within the same species, 96% at the subspecies level, and 73-96% (mean 85%) at the interspecies level (and 98% in the c ...200111594590
bacteriocin susceptibility of gardnerella vaginalis and its relationship to biotype, genotype, and metronidazole susceptibility.our purpose was to investigate the bacteriocin susceptibility of gardnerella vaginalis and its relationship to biotype, genotype, and resistance to metronidazole.200111717655
identification of a gardnerella vaginalis hemoglobin-binding protein.previous studies have shown that gardnerella vaginalis can utilize human hemoglobin as a sole source of iron. in this study, the interaction between human hemoglobin and g. vaginalis cells was investigated. with a solid phase dot blot assay, g. vaginalis cells were shown to bind digoxigenin (dig)-labeled human hemoglobin. a human hemoglobin-binding protein with an estimated molecular weight of 124 kilodaltons (kda) was detected by western blot analysis of g. vaginalis proteins. the hemoglobin-bi ...200111116397
vaginal microflora associated with bacterial vaginosis in nonpregnant women: reliability of sialidase detection.to determine the prevalence of gardnerella vaginalis, anaerobic bacteria and mycoplasma hominis in vaginal specimens of women with and without bacterial vaginosis (bv) as well as to determine the sensitivity and specificity of the direct sialidase assay of vaginal fluid as a rapid test for diagnosing this syndrome.200111368254
antimicrobial protein produced by vaginal lactobacillus acidophilus that inhibits gardnerella vaginalis.to isolate bacteriocin from a vaginal strain of lactobacillus acidophilus.200111368257
bacterial vaginosis among a group of married jordanian women: occurrence and laboratory diagnosis.a total of 310 vaginal swabs collected from a group of married jordanian women complaining of vaginal discharge were examined for bacterial vaginosis. the scoring system of nugent for the interpretation of gram staining was employed. this system revealed the presence of the condition in 29.7% of patients. results obtained using the scoring system correlated significantly with the detection of clue cells and the scarcity of white blood cells in the vaginal discharge. an inverse relationship was f ...200111368266
human immunodeficiency virus type 1 stimulatory activity by gardnerella vaginalis: relationship to biotypes and other pathogenic characteristics.stimulation of human immunodeficiency virus (hiv) type 1 expression by gardnerella vaginalis is one possible cause for an increase in the amount of virus in the genital tract. the ability of g. vaginalis to induce hiv expression in chronically infected u1 cells was investigated, along with its possible relationship to biotype, genotype, and resistance to metronidazole and bacteriocin. significant hiv stimulatory activity was found in 5 (50%) lysates of g. vaginalis. the ability to induce hiv exp ...200111398105
nugent score related to vaginal culture in pregnant women.to relate gram-stained smears, using the nugent criteria, to quantitative and qualitative vaginal cultures in pregnant women.200111430961
defense factors of vaginal lactobacilli.to determine the antagonistic relationship between vaginal lactobacilli and endogenous vaginal microflora.200111518895
relationship of abnormal vaginal flora, proinflammatory cytokines and idiopathic infertility in women undergoing ivf.to investigate the prevalence of bacterial vaginosis (bv) and abnormal bacterial vaginal flora in an infertile population and correlate with cervical cytokine production and in vitro fertilization (ivf) outcome.200111584481
gluteal necrotizing myofascitis: an unusual delayed complication of abdominal sacrocolpopexy.persistent low back pain developed in a 51-year-old woman after she had undergone abdominal sacrocolpopexy. four months postoperatively, necrotizing myofascitis developed in her gluteal muscles. the infected mesh, which had eroded into the vagina, was removed. cultures of the infected mesh and abscesses grew common vaginal flora, including gardnerella vaginalis and actinomyces.200111717673
evaluation of the affirm ambient temperature transport system for the detection and identification of trichomonas vaginalis, gardnerella vaginalis, and candida species from vaginal fluid specimens.the objective of this study was to measure the performance of the affirm ambient temperature transport system (atts) over time and to estimate the length of time the system can preserve a vaginal specimen containing the three common organisms causing vaginitis: trichomonas vaginalis, candida species, and gardnerella vaginalis (one of the causative agents of bacterial vaginosis). women with symptoms of vaginitis presenting to one of three clinical centers were evaluated over a 4- to 8-week period ...200111526150
gardnerella vaginalis isolated from patients with bacterial vaginosis and from patients with healthy vaginal ecosystems.the differences in the phenotype and genotype of gardnerella vaginalis isolates from patients with bacterial vaginosis (bv) and from patients without bv are unknown. in our study, 43 isolates of g. vaginalis were examined for biotype (hippurate hydrolysis, lipase, and beta-galactosidase activity), sensitivity to metronidazole, and genotype. of the 117 women visiting the gynecology clinic at rush-presbyterian-st. luke's medical center who were included in the study, 27.4% were found to have bv. g ...200111528575
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