Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| semen culture, leukocytospermia, and the presence of sperm antibodies in seminal hyperviscosity. | seminal hyperviscosity is generally thought to reveal genitourinary infection. the aim of the present work was to study this hypothesis. a total of 65 semen samples were obtained from males presenting for infertility screening. the samples were evaluated according to who criteria and microbiologically investigated, including culturing for mycoplasma hominis and ureaplasma urealyticum, and microscopic observation of chlamydia trachomatis by a direct fluorescence assay. determination of local anti ... | 1999 | 9973141 |
| evaluation of the etest for susceptibility testing of mycoplasma hominis and ureaplasma urealyticum. | the etest was used for antibiotic susceptibility testing of mycoplasma hominis and ureaplasma urealyticum isolates and the results were compared with those obtained with the broth microdilution method. for 50 clinical isolates of m. hominis the mics of doxycycline, ofloxacin and ciprofloxacin agreed within +/- one dilution and +/- two dilutions in 82-98% and 98-100% of cases, respectively. the mics of erythromycin, azithromycin, doxycycline, ofloxacin and ciprofloxacin were evaluated for 50 clin ... | 1999 | 10350390 |
| isolation pattern and clinical outcome of genital mycoplasma in neonates from a tertiary care neonatal unit. | the role of genital mycoplasma in perinatal mortality and morbidity has been debated. this study was undertaken to determine the frequency of isolation of genital mycoplasma and evaluate its association with clinical outcome. sixty-six cerebrospinal fluid (csf) and 49 tracheal aspirates taken from 100 low birthweight infants who had suspected meningitis and/or respiratory distress respectively were cultured for genital mycoplasma. ureaplasma urealyticum was isolated from 9 per cent of csf and 14 ... | 1999 | 10401191 |
| comparative in-vitro activity of levofloxacin, other fluoroquinolones, doxycycline and erythromycin against ureaplasma urealyticum and mycoplasma hominis. | the susceptibility of 56 ureaplasma urealyticum and 57 mycoplasma hominis strains to levofloxacin, ofloxacin, ciprofloxacin, fleroxacin, doxycycline and erythromycin was determined by an agar dilution method. the reference strain used was m. hominis pg 21. agar plates containing serial dilutions of antibiotics (range 0.03-16 mg/l), and control plates (without antibiotics) were inoculated with bacteria suspended in modified shepard's broth using a multipoint inoculator. levofloxacin showed greate ... | 1999 | 10404335 |
| [genetic variability of mycoplasma during interaction with host: vertical transfer and rearrangement in genes of surface antigenic determinants]. | 1999 | 10420256 | |
| detection of viral and bacterial infections in women with normal and abnormal colposcopy. | signs and symptoms of sexually-transmitted diseases (std) do not allow any etiological diagnosis in women. colposcopic findings are seldom pathognomic. consequently, the microbiology laboratory with the recent availability of molecular diagnostic tools is required to detect the infectious bacterial and/or viral agents involved in std. in cervical samples of women submitted to gynaecological screening for past or present signs and symptoms of inflammation and with different colposcopic findings, ... | 1999 | 10422688 |
| relationship between ureaplasma urealyticum vaginal colonization and polymorphism in the interleukin-1 receptor antagonist gene. | the relationship between polymorphisms in the interleukin-1 receptor antagonist (il-1ra) gene and microbial vaginal colonization was examined in 88 asymptomatic women of reproductive age. alleles of the intron 2 region of the il-1ra gene were identified by polymerase chain reaction (pcr). pcr was also used to detect ureaplasma urealyticum, mycoplasma hominis, and candida albicans; bacterial vaginosis (bv) was identified by clinical criteria. among the 31 women with vaginal u. urealyticum, only 3 ... | 1999 | 10438393 |
| the adherence-associated lipoprotein p100, encoded by an opp operon structure, functions as the oligopeptide-binding domain oppa of a putative oligopeptide transport system in mycoplasma hominis. | mycoplasma hominis, a cell-wall-less prokaryote, was shown to be cytoadherent by the participation of a 100-kda membrane protein (p100). to identify the gene encoding p100, peptides of p100 were partially sequenced to enable the synthesis of p100-specific oligonucleotides suitable as probes for the detection of the p100 gene. with this strategy, we identified a genomic region of about 10. 4 kb in m. hominis fbg carrying the p100 gene. analysis of the complete deduced protein sequence suggests th ... | 1999 | 10438757 |
| scanning electron microscopy of endometrial biopsies of patients with bacterial vaginosis shows morphology resembling mycoplasma/ureaplasma organisms. | 1999 | 10448406 | |
| mycoplasma hominis bacteraemia not associated with genital infections. | we describe two cases of systemic infection with m. hominis without prior genital infection and review the previous literature on this condition. | 1999 | 10468136 |
| [clinicobacteriological studies on the nine cases with upper genital tract mycoplasma hominis infection]. | clinicobacteriological characteristics of nine cases isolated mycoplasma hominis from the genital tract were studied, and the following results were obtained: elevation of igg antibodies to m. hominis was measured by elisa in all cases, but in the mi method only one case showed an elevation of metabolic inhibitory antibody. convalescent sera from seven patients showed additional and high density bands which were not recognized by acute phase sera in immunoblotting. it was thought that in two pat ... | 1999 | 10481399 |
| amplified-fragment length polymorphism fingerprinting of mycoplasma species. | amplified-fragment length polymorphism (aflp) is a whole-genome fingerprinting method based on selective amplification of restriction fragments. the potential of the method for the characterization of mycoplasmas was investigated in a total of 50 strains of human and animal origin, including mycoplasma genitalium (n = 11), mycoplasma pneumoniae (n = 5), mycoplasma hominis (n = 5), mycoplasma hyopneumoniae (n = 9), myco plasma flocculare (n = 5), mycoplasma hyosynoviae (n = 10), and mycoplasma di ... | 1999 | 10488196 |
| sparfloxacin selects gyrase mutations in first-step mycoplasma hominis mutants, whereas ofloxacin selects topoisomerase iv mutations. | the role of mutations in the genes for gyra and parc in quinolone resistance in mycoplasma hominis was studied. selection with sparfloxacin gave mutations at gyra83 (ser-->leu; escherichia coli numbering) or gyra87 (glu-->lys), and mutants had increased levels of resistance to sparfloxacin (8- to 16-fold) but not to ofloxacin. selection with ofloxacin gave changes at parc80 (ser-->ile) or parc84 (glu-->lys), and mutants were four- to eightfold more resistant to ofloxacin but not to sparfloxacin. ... | 1999 | 10508030 |
| in vitro susceptibilities of mycoplasma hominis to six fluoroquinolones as determined by e test. | twenty isolates of mycoplasma hominis were tested for their susceptibility to six fluoroquinolones by the e test. the mics at which 90% of the isolates were inhibited (in micrograms per milliliter) were as follows: sparfloxacin, 0.031; clinafloxacin, moxifloxacin, and trovafloxacin, 0.063; levofloxacin, 0.25; and ciprofloxacin, 0.5. increasing the amount of inoculum or incubation in co(2) elevated mics by </=1 dilution. e tests produce fluoroquinolone mics comparable to those obtained by agar an ... | 1999 | 10508049 |
| association of indicators of bacterial vaginosis with a female genital tract factor that induces expression of hiv-1. | the aim of this study was to determine the relationship of bacterial vaginosis and bacterial vaginosis-associated microorganisms with an hiv-inducing factor (hif) found in cervicovaginal lavage. | 1999 | 10513649 |
| a double-blind, randomized, controlled multicentre study to compare the efficacy of ciprofloxacin with pivampicillin as oral therapy for epididymitis in men over 40 years of age. | to compare the efficacy and safety of ciprofloxacin 500 mg orally twice daily with pivampicillin 700 mg orally twice daily for 10 days in men with acute epididymitis and over 40 years of age. | 1999 | 10532980 |
| evaluation of igg, igm, and iga antibodies to mycoplasma penetrans detected by elisa and immunoblot in hiv-1-infected and std patients, in são paulo, prazil. | the aim of the present study was to determine the frequency of igg, iga, and igm antibodies to mycoplasma penetrans in hiv-1-infected patients and in patients with sexually transmitted diseases. we tested serum samples from 106 hiv-1-positive patients and 110 individuals with clinical symptoms of urethritis. elisa and the immunoblot test were performed using m. penetrans lipid associated membrane proteins as antigen. by elisa, we found a higher frequency (p < 0.05) of igg against m. penetrans in ... | 1999 | 10572313 |
| life-threatening mycoplasma hominis mediastinitis. | mycoplasma hominis infections are easily missed because conventional methods for bacterial detection may fail. here, 8 cases of septic mediastinitis due to m. hominis are reported and reviewed in the context of previously reported cases of mediastinitis, sternum wound infection, pleuritis, or pericarditis caused by m. hominis. all 8 patients had a predisposing initial condition related to poor cardiorespiratory function, aspiration, or complications related to coronary artery surgery or other th ... | 1999 | 10585808 |
| antimycoplasmal activities of (s)-(-)-9-fluoro-2,3-dihydro-3-methyl-10 -[4-(2-pyridyl)-1-piperazinyl]-7-oxo-7h-pyrido[1,2,3-de][1,4]benzoxazine -6-carboxylic acid (yh-6) in comparison with other antibiotics in vitro. | to determine the susceptibilities of mycoplasma and ureaplasma to (s)-(-)-9-fluoro-2,3-dihydro-3-methyl-10 -[4-(2-pyridyl)-1-piperazinyl]-7-oxo-7h-pyrido[1,2,3-de][1,4]benzoxazine -6-carboxylic acid (yh-6) and to compare it with those referential quinolones, macrolides, and tetracyclines. | 1999 | 11270970 |
| [study on etiology of nongonococcal urethritis]. | one thousand one hundred and fifty-seven samples from urogenital tract were detected by isolating culture and immunofluorescence. the positive rates of chlamydia trachomatis (ct), ureaplasma urealyticum (uu), mycoplasma hominis (mh), candida (cd), staphylococcus aureus were 27.8%, 33.5%, 25.6%, 11.5%, 23% respectively. the rate of mixed infection caused by at least two pathogens was 19.8%. about 90% of the infected patients were from 20 to 40 years old. the related factors of pathogen detection ... | 1999 | 12016794 |
| colonization and transmission of ureaplasma urealyticum and mycoplasma hominis from mothers to full and preterm babies by normal vaginal delivery. | a prospective study was carried out among pregnant women and their newborn babies in the university hospital, kuala lumpur from january 1996 to june 1997. the maternal cervical colonization rates of ureaplasma urealyticum (uu) and mycoplasma hominis (mh) were found to be 57.5% and 15.8% respectively while the isolation rates from nasopharyngeal secretions of the newborns were 50.8% for uu and 6.6% for mh. the overall transmission rates were 88.4% for uu and 42.1% for mh. there was no significant ... | 1999 | 10972036 |
| association of ureaplasma urealyticum colonization in male urethra and condyloma acuminatum. | the frequent coexistence of two or more sexually transmitted diseases in one patient has been reported in non-dermatological literature, mostly in languages other than english. identification of ureaplasma urealyticum, chlamydia trachomatis and mycoplasma hominis in men with other stds is important, since these bacteria have been implicated in a variety of diseases such as non-gonococcal urethritis, premature rupture of fetal membranes, and infertility in female sexual partners of these patients ... | 2000 | 10979348 |
| characterization of the variability of a 75-kda membrane protein in mycoplasma hominis. | the gene p75 encoding a 75-kda surface-exposed membrane protein p75 was cloned and sequenced from mycoplasma hominis type strain pg21t. to investigate the intraspecies variability, sequences were obtained from an additional two isolates 7488 and 183, and the three sequences were compared. the nucleotide and amino acid differences were not confined to specific regions of the gene/protein, but when comparing the three sequences, differences were present as single site substitutions or small insert ... | 2000 | 10981709 |
| cloning and nucleotide sequence of the dna gyrase (gyra) gene from mycoplasma hominis and characterization of quinolone-resistant mutants selected in vitro with trovafloxacin. | we report the cloning and characterization of the gyra gene of the mycoplasma hominis dna gyrase, which was previously shown to be associated with quinolone resistance in this organism. the 2,733-bp gyra gene encodes a protein of 911 amino acids with a calculated molecular mass of 102.5 kda. as expected, m. hominis gyra exhibits higher homology with the gyra subunits of the gram-positive bacteria clostridium acetobutylicum, bacillus subtilis, streptococcus pneumoniae, and staphylococcus aureus t ... | 2000 | 10991851 |
| genital mycoplasmas among pregnant women in cóte d'ivoire, west africa: prevalence and risk factors. | a cross-sectional study was conducted among pregnant women in côte d'ivoire to assess the prevalence and the factors associated with mycoplasma colonization. a positive culture was found in 400 of 551 women (73%); ureaplasma urealyticum was identified in 22%, mycoplasma hominis in 20% and both microorganisms in 31%. mycoplasma colonization was not associated with genital symptoms or signs. young age, low educational level, antimicrobial chemotherapy before consultation and presence of bacterial ... | 2000 | 10997504 |
| role of the male partner in the lower genitourinary tract infection of female. | we studied the relationships of selected microbes and the role of consorts in the causation of vaginal discharge which may be due to cervicitis or vaginitis. a total of 93 consecutive patients in the reproductive age group with symptoms of vaginal discharge along with their sexual partners were studied. samples were collected from the cervix and posterior fornix of the female patients and from the urethra and sub-prepucial area of the male partner for culture of neisseria gonorrhoeae, gardnerell ... | 2000 | 11006655 |
| prosthetic valve endocarditis caused by mycoplasma hominis. | mycoplasma hominis endocarditis is extremely uncommon and difficult to diagnose. atypical growth characteristics in routine bacterial culture and an inability to demonstrate the organism using gram staining can lead to a delayed diagnosis of mycoplasma hominis infections, and the organism is often missed. this report describes a patient with mycoplasma hominis prosthetic valve endocarditis. the microorganism was recovered from the mitral prosthesis but was missed in blood cultures. this finding ... | 2000 | 11014630 |
| [laboratory diagnosis of mycoplasma infections in man]. | 2000 | 11031436 | |
| [the effect of velikobagachanskaia mineral water on the course of chronic pyelonephritis]. | the employing of velikobagachanskaia mineral water internally by drinking and externally by taking a bath with it adds to the effectiveness of treatment of chronic pyelonephritis, modulates parameters associated with immune homeostasis, reduces the number of carriers of antibodies to m. hominis, facilitates prolongation of clinical and laboratory remission. | 2000 | 11031464 |
| distribution of ecto 5'-nucleotidase on mycoplasma species associated with arthritis. | the enzyme ecto 5'-nucleotidase (5'n) was found to be active on 8/14 strains of mycoplasma fermentans, k(m) (+/-s.d.) 3.8+/-2.8 microm 5'-amp, and on the type strain of mycoplasma pulmonis, k(m) 0.63 microm 5'-amp. the six m. fermentans strains lacking 5'n activity were related by restriction fragment length polymorphism typing. at ph 8.5, the type strains of mycoplasma arthritidis, mycoplasma buccale and ureaplasma urealyticum showed a relatively non-specific phosphatase activity against 5'-amp ... | 2000 | 11040429 |
| recurrent familial neonatal deaths: hereditary surfactant protein b deficiency. | hereditary surfactant protein b (sp-b) deficiency is an uncommon autosomal recessive lung disorder that causes hypoxemic respiratory failure in mature, morphologically normal infants. recognition and diagnosis of this condition is of paramount importance, as it has significant implications for future pregnancies with a recurrence risk of 25%. in a family with three neonatal deaths over 20 years, sp-b deficiency was diagnosed following the death of the fourth affected infant. previous deaths were ... | 2000 | 11041444 |
| induction of monocyte chemoattractant protein-1 by mycoplasma hominis in respiratory epithelial cells. | very small preterm infants who have genital mycoplasmas isolated from the trachea are at increased risk to develop bronchopulmonary dysplasia (bpd). the early stages of bpd are characterized by inflammation. recruitment and activation of mononuclear cells in response to mycoplasmas may be important in the early stage of the disease. lung epithelial cell production of monocyte chemoattractant protein-1 (mcp-1), a protein that attracts and activates mononuclear cells, could be critical in the regu ... | 2000 | 11094869 |
| mycoplasma hominis peritonitis after renal transplantation. | 2000 | 11124622 | |
| hematoma infection with mycoplasma hominis following transplant nephrectomy. | 2000 | 11168067 | |
| mycoplasma hominis catheter-related infection in a patient with multiple trauma. | 2000 | 11168084 | |
| mycoplasma fermentans, m. hominis, and m. hyorhinis inhibit infectivity and growth of chlamydia trachomatis and c. pneumoniae in hep-2 cells. | 2000 | 11184179 | |
| [analysis of regions determining resistence to fluoroquinolones in genes gyra and parc in clinical isolates of mycoplasma hominis]. | fifteen strains of m. hominis isolated from patients with urogenital inflammations were analyzed. variations in the quinolone resistance-determining regions (qrdr) have been found in fluoroquinolone-resistant m. hominis clinical isolates in comparison with the reference pg21 strain. in one isolate, parc had asn substitute at position 91. | 2000 | 11186458 |
| immunoelectrophoretic analysis of medium-derived antigens bound to the surface of mycoplasma hominis, mycoplasma fermentans and ureaplasma urealyticum. | we analyzed mycoplasma hominis, mycoplasma fermentans and ureaplasma urealyticum antigens by immunochemical methods. the immunochemical reaction showed that all three species bound to the surface of one medium component, which origin stemmed from the horse serum. this substance functioned as one of the most potent components of mycoplasma immunogens for all three species. | 2000 | 11200673 |
| [comparison of various pcr methods for the detection of chlamydia trachomatis, ureaplasma urealyticum, and mycoplasma hominis in women with impaired reproductive function]. | the comparative evaluation of the pcr test "polimik" (research and production firm "litekh", moscow) and the pcr test of the novosibirsk institute of bioorganic chemistry (nibc) was carried out. the results obtained with the use of the pcr test "polimik" and the pcr test of the nibc of the detection of c. trachomatis and m. hominis coincided in 97.8% and 97.4% of cases. for u. urealyticum, the coincidence of the results of both pcr tests was 81.2%. among women who visited gynecologists for repro ... | 2000 | 11210648 |
| effect of intravaginal clindamycin cream on pregnancy outcome and on abnormal vaginal microbial flora of pregnant women. | to determine whether intravaginal clindamycin cream reduces the incidence of abnormal pregnancy outcome in women with abnormal vaginal microbial flora graded as intermediate or bv and to investigate the effect of the antibiotic on vaginal microbial flora. | 2000 | 10968599 |
| relevant prevalence of mycoplasma hominis and ureaplasma urealyticum serogroups in hiv-1 infected men without urethritis symptoms. | m. hominis and u. urealyticum are the better-known mycoplasma species pathogenic to the human genitourinary tract, causing mainly urethritis, bacterial vaginosis and pregnancy complications. in hiv-infected patients, the prevalence and role of these species is still not well known. the aim of this work was to determinate the prevalence of these species in this group of male patients (hiv group), in comparison to a group of men with clinical symptoms of urethritis (std group). m. hominis was isol ... | 2000 | 10968880 |
| [the influence of anaerobic bacteria and mycoplasma isolated from the genital tract on mast cell activation]. | nowadays, it is known that mast cells, numerously appearing in all organs and being a source of a wide range of mediators and cytokines, are involved both in physiological and pathological processes. the aim of our study was to examine whether vaginal bacteria, especially those participating in bacterial vaginosis, are able to activate mast cells to mediators secretion. the study was done on rat peritoneal mast cells. the mast cells were incubated in vitro with suspensions of bacteroides capillo ... | 2000 | 11286181 |
| mycoplasmal infections alter gene expression in cultured human prostatic and cervical epithelial cells. | to better understand how infections by mycoplasmas affect gene expression in human cells, we quantitatively measured the transcripts of 38 cytokine genes in hpv e6- and e7-immortalized cervical and prostatic epithelial cells before and after infection by four human urogenital mycoplasmas, m. fermentans, m. genitalium, m. hominis and m. penetrans. using the multi-probe rnase protection assay (rpa), 22 and 23 cytokine gene transcripts were detected in the non-infected control prostatic and cervica ... | 2000 | 10617789 |
| association of chronic urinary symptoms in women and ureaplasma urealyticum. | objectives. to determine the incidence of ureaplasma urealyticum in women experiencing chronic urinary symptoms and to determine whether antibiotic therapy targeting these organisms is effective. | 2000 | 10736488 |
| genetic basis of natural resistance to erythromycin in mycoplasma hominis. | 2000 | 10747837 | |
| [the use of in situ polymerase chain reaction for detection the intracellular localization of mycoplasma hominis in cultured hela cells]. | mycoplasma hominis was incubated with hela cells up to 72 h. infection of hela cells was estimated by in situ polymerase chain reaction with a subsequent use of computer digital image analysis for result processing. the localization of mycoplasma in the cell nucleus was demonstrated after a 48 h incubation. the quantity of infected hela cells was increased with time to make 58 and 75% on days 2 and 3, respectively. no m. hominis was found in the cytoplasma of hela cells. | 2000 | 10752127 |
| effect of some psychotropic drugs and a barbiturate on mycoplasmas. | the inhibitory effect of selected membrane stabilisers on mycoplasma pneumoniae, m. hominis and ureaplasma urealyticum was investigated in vitro. the phenothiazine chlorpromazine (cpz) and the barbiturate thiopental (leopental(r)) as well as the stereo-isomeric thioxanthene derivatives; cis(z)-clopenthixol (zu-clopenthixol(r))/ trans (e)-clopenthixol and cis (z)-chlorprothixen (truxal(r))/trans(e)-chlorprothixen, all have antimycoplasmal effect in the range 3.9-312 mg/l, measured as growth inhib ... | 2000 | 10773494 |
| [in vitro sensitivity to antibiotics in 178 strains of genital mycoplasma isolated from gynecology consultants in dakar]. | the susceptibility to antibiotics of 144 strains of ureaplasma urealyticum and 34 strains of mycoplasma hominis isolated in dakar, senegal, was determinated by mic determination in a medium. doxycyclin and minocyclin are active on more than 90% of the strains of u. urealyticum, and more than 80% of m. hominis strains. over 93% of u. urealyticum strains are susceptible to all the macrolids and apparented tested (erythromycin, pristinamycin, josamycin), but the activity of lincomycin, pristinamyci ... | 2000 | 10774483 |
| [transformation of mycoplasma hominis by plasmid pam120 using electroporation]. | mycoplasma hominis was transformed by electroporation with plasmid pam120 containing the transposon tn916 that carried the tetm gene responsible for the resistance to tetracycline. the frequency of transformation was 10(-7)-10(-8) colony-forming units (cfu) per 10 micrograms of plasmid dna. the pcr analysis of transformed dna confirmed the transposon integration into the mycoplasma genome. | 2000 | 10779903 |
| [subdural empyema due to mycoplasma hominis following epidural anesthesia]. | in the literature there are sporadic reports of spinal epidural abscesses after epidural anaesthesia (staphylococcus aureus in 82%), whilst subdural empyemas are more often related to ear and sinus conditions. | 2000 | 10789141 |
| in vitro activity of a cellulose acetate phthalate topical cream against organisms associated with bacterial vaginosis. | 2000 | 10797102 | |
| association of ureaplasma urealyticum with abnormal reactive oxygen species levels and absence of leukocytospermia. | ureaplasma urealyticum is a commensal of the lower genitourinary tract of many sexually active adults. the organism is more common in partners of infertile than fertile marriages. we conducted a prospective study at our tertiary care center to confirm a possible association between u. urealyticum and abnormal sperm function parameters. | 2000 | 10799180 |
| [mixed urogenital infection in men]. | high occurrence of sexually transmitted diseases (std) caused by a combination of different infective agents was established. in 62.5% of cases such diseases took a course typical for mixed infections. combinations of infective agents of std were found to have several typical associations, e.g. ureaplasma urealyticum and mycoplasma hominis in combination with opportunistic microflora. the microbiocenosis of the reproductive tract was shown to form under the influence of a complex of endogenous ( ... | 2000 | 10808589 |
| comparative potency of gemifloxacin, new quinolones, macrolides, tetracycline and clindamycin against mycoplasma spp. | this study determined the comparative in vitro potency of the new investigative quinolone gemifloxacin (sb-265805) using low-passaged clinical isolates and type strains of mycoplasma commonly found in the human respiratory and urogenital tracts. organisms studied were mycoplasma pneumoniae, mycoplasma hominis, mycoplasma fermentans, mycoplasma genitalium, mycoplasma penetrans and ureaplasma urealyticum, obtained from different geographical regions. comparator drugs were levofloxacin, trovafloxac ... | 2000 | 10824029 |
| updated phylogenetic description of the mycoplasma hominis cluster (weisburg et al. 1989) based on 16s rdna sequences. | the fastidious nature of the mollicutes (mycoplasmas), their lack of a classic bacterial cell wall, and their very small genome, make phylogenetic placements of new species in this enlarging group of prokaryotes an important and valuable aid in their classification. in this report we have determined the phylogeny of the mycoplasma hominis cluster of the hominis group. the 16s rdna sequences from several previously described mycoplasma species were determined and ten species were found to belong ... | 2000 | 10826816 |
| observations on the antibiotic treatment of experimentally induced mycoplasmal infections in mice. | antibiotics were given subcutaneously to female mice, colonized by mycoplasmas in the vagina and by others in the oropharynx. mycoplasma pulmonis was eradicated from the vagina of 36 of 42 immunocompetent to or balb/c mice with oxytetracycline, and from 17 of 18 to mice with lymecycline. mycoplasma hominis was eradicated from the vagina of all of 18 immunocompetent balb/c mice with oxytetracycline or tetracycline. regarding oropharyngeal organisms, m. pulmonis was eradicated from only 20 of 42 t ... | 2000 | 10837449 |
| host and tissue specificity of trichomonas vaginalis is not mediated by its known adhesion proteins. | adhesion of trichomonas vaginalis is believed to be dependent on four adhesion proteins, which are thought to bind to vaginal epithelial cells in a specific manner with a ligand-receptor type of interaction. however, the specific receptors on the host cell have not yet been identified. in this work, the ability of the t. vaginalis adhesins to bind to cells of different histologic derivations and from different species has been studied. hela, cho, and vero cell lines; erythrocytes from different ... | 2000 | 10858260 |
| comparative activities of telithromycin (hmr 3647), levofloxacin, and other antimicrobial agents against human mycoplasmas. | the activities of telithromycin and levofloxacin against 99 mycoplasma strains were compared to those of several macrolides, ofloxacin, and doxycycline. telithromycin mics of </=0.25 microgram/ml were found for all isolates, except for mycoplasma hominis, while levofloxacin was active at concentrations of </=1 microgram/ml against all species studied. | 2000 | 10858366 |
| accurate detection of male subclinical genital tract infection via cervical culture and dna hybridization assay of the female partner. | the accuracy of the pace2 dna hybridization assay of the cervix and cervical culture in female partners for the diagnosis of male subclinical genital tract infection were assessed in a male infertility population. a total of 184 men were screened for the presence of chlamydia trachomatis, ureaplasma urealyticum and mycoplasma hominis. seventy-one men were identified with a positive test for one or more of the above mentioned micro-organisms. the overall prevalence of bacterial infection was 39%. ... | 2000 | 10632761 |
| cloning, sequencing and variability analysis of the gap gene from mycoplasma hominis. | the gap gene encodes the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (gapdh). the gene was cloned and sequenced from the mycoplasma hominis type strain pg21(t). the intraspecies variability was investigated by inspection of restriction fragment length polymorphism (rflp) patterns after polymerase chain reaction (pcr) amplification of the gap gene from 15 strains and furthermore by sequencing of part of the gene in eight strains. the m. hominis gap gene was found to vary more than ... | 2000 | 10650196 |
| effect of a mycoplasma hominis-like mycoplasma on the infection of hep-2 cells by the tw-183 strain of chlamydia pneumoniae. | we isolated a mycoplasma hominis-like mycoplasma from a stock culture of chlamydia pneumoniae tw-183 obtained from the american type culture collection and eradicated the contaminant by treating the stock suspension with a nonionic detergent, igepal ca-630. the m. hominis-like mycoplasma neither inhibits nor enhances the infectivity of c. pneumoniae for hep-2 cells. | 2000 | 10655399 |
| detection of microorganisms in the tracheal aspirates of preterm infants by polymerase chain reaction: association of adenovirus infection with bronchopulmonary dysplasia. | bronchopulmonary dysplasia (bpd) is recognized as an important cause of morbidity and mortality in preterm infants. because the role of congenital infections in bpd has been debated, the purpose of this study was to test the hypothesis that detection of infectious agents in tracheal aspirate samples was associated with the development of bpd. tracheal aspirate samples were obtained within the 1st week of life and screened by polymerase chain reaction for adenovirus, cytomegalovirus, parvovirus, ... | 2000 | 10674351 |
| retroperitoneal abscess and bacteremia due to mycoplasma hominis in a polytraumatized man. | we report a case of a retroperitoneal abscess due to mycoplasma hominis in a young polytraumatized man who developed septicemia under treatment with rifampin and flucloxacillin. m. hominis was recovered from blood cultures as well as from the abscess near the left iliac spine. after 10 days of therapy with clindamycin the patient improved, and intraoperatively taken swabs were culture negative but still positive by pcr. | 2000 | 10697792 |
| in vitro activity of gemifloxacin (sb 265805; lb20304a) against human mycoplasmas. | the in vitro activity of gemifloxacin, a new broad-spectrum fluoroquinolone, was compared with those of ciprofloxacin, erythromycin, azithromycin and doxycycline against 29 human respiratory or urogenital tract mycoplasmas. gemifloxacin was highly active against all of the mycoplasma and ureaplasma species tested (mic range 0.001-0.25 mg/l) and was 5- to 100-fold more active than ciprofloxacin. doxycycline was less active than gemifloxacin against the mycoplasmas (mic range 0.01-1 mg/l) but had ... | 2000 | 10702559 |
| molecular comparison of mycoplasma hominis strains isolated from colonized women and women with various urogenital infections. | twenty mycoplasma hominis strains isolated from colonized women and women with various urogenital infections were investigated for genetic and antigenic homogeneity by different methods. restriction fragment length polymorphism analysis demonstrated heterogeneity for all strains, with one exception. two strains sequentially isolated from one patient showed identical patterns. otherwise, no clonal clustering could be detected within the strains isolated from either of the diagnostic groups. in co ... | 2000 | 10705620 |
| molecular dissection of mycoplasma hominis. | m. hominis is commonly found as part of the normal flora in the female genital tract, but several studies have shown that it may be involved in a variety of urogenital infections. the basis for clinical manifestations in some patients has varyingly been attributed to host and m. hominis factors. the host factors involved in the infection process are largely unknown. m. hominis have no cell wall and outer membranes, and at present it seems plausible that m. hominis possesses genetic systems allow ... | 2000 | 10721331 |
| single dose oral azithromycin versus seven day doxycycline in the treatment of non-gonococcal mucopurulent endocervicitis. | the aim of this study was to compare single dose oral azithromycin versus seven-day doxycycline in the treatment of non-gonococcal mucopurulent cervicitis (mpc). one hundred and thirty-one women with non-gonococcal mpc were enrolled in a prospective-randomised study to compare the efficacy and safety of a single oral dose of 1 g azithromycin and a seven-day course of 100 mg doxycycline twice daily. clinical examination and culture samples for chlamydia trachomatis and other microorganisms were p ... | 2000 | 10870778 |
| the association of mycoplasma hominis, ureaplasma urealyticum and mycoplasma genitalium with bacterial vaginosis: observations on heterosexual women and their male partners. | the prevalence of 3 mycoplasmas (mycoplasma hominis, ureaplasma urealyticum and mycoplasma genitalium) was determined in a cohort of women with or without bacterial vaginosis (bv) and in their respective male partners. heterosexual women with or without bv and their male partners were recruited and genital sampling for these microorganisms was performed. seventeen women with bv and 21 women with normal flora, and their respective male partners, were recruited. m. hominis was present in 9 (53%) o ... | 2000 | 10872907 |
| the prevalence of urethral infections amongst asymptomatic young men in hat yai, southern thailand. | the aim of this study was to survey sexual behaviour and estimate the prevalence of urethral infections amongst male vocational college students. a cross-sectional survey was performed among 479 young men attending 2 vocational colleges in hat yai, southern thailand. polymerase chain reaction (pcr) tests of first-void urine (fvu) samples were used to detect infection with chlamydia trachomatis, neisseria gonorrhoeae, ureaplasma urealyticum, mycoplasma genitalium and mycoplasma hominis. girlfrien ... | 2000 | 10872914 |
| [neonatal infections with genital mycoplasmas]. | 2000 | 10904752 | |
| [biological diagnosis of infections caused by mycoplasmas]. | 2000 | 10904755 | |
| [bacterial vaginosis. epidemiology and risk factors]. | bacterial vaginosis (bv) is a change in vaginal ecosystem where lactobacilli dominate, flora is absent or greatly reduced, and replaced with a mixed, predominantly anaerobic flora, consisting of gardnerella vaginalis, mycoplasma hominis, mobiluncus spp, bacteroides spp, prevotela spp, peptostreptococcus spp, fusobacterium spp and porphyromonas spp. the concentration of bacteria increases from 100 to 1000 fold in women with bv compared to healthy women. bv has been formerly known as nonspecific v ... | 2000 | 10916461 |
| cytokine concentrations in seminal plasma from subfertile men are not indicative of the presence of ureaplasma urealyticum or mycoplasma hominis in the lower genital tract. | the inflammatory response to the presence of ureaplasma urealyticum or mycoplasma hominis in the lower genital tract of subfertile men without any signs or symptoms of infection was investigated by measuring the concentrations of interleukin (il)-6, il-8, tumour necrosis factor-alpha (tnf-alpha) and interferon-gamma (ifn-gamma) in seminal plasma. semen samples were collected from 30 culture-positive subfertile males and 23 culture-negative subfertile males. enzyme-linked immunosorbent assays sho ... | 2000 | 10933253 |
| relationship of bacterial vaginosis and mycoplasmas to the risk of spontaneous abortion. | this study was undertaken to investigate a possible link between first-trimester diagnosis of bacterial vaginosis and cessation of pregnancy at < or =20 weeks' gestation. | 2000 | 10942482 |
| association of mycoplasma hominis and ureaplasma urealyticum with some indicators of nonspecific vaginitis. | the purpose of this study was to determine the isolation rates of mycoplasma hominis and ureaplasma urealyticum from three populations of women and also to relate the presence of these microorganisms with some indicators of nonspecific vaginitis. three hundred vaginal swabs were taken from delivery, pregnant and control (not pregnant) women. cultures were done in e broth supplemented with arginine or urea. m. hominis was isolated in 5% at delivery, 12% from pregnant and 5% from control women and ... | 2000 | 10948822 |
| in vitro activity of trovafloxacin compared to those of five antimicrobials against mycoplasmas including mycoplasma hominis and ureaplasma urealyticum fluoroquinolone-resistant isolates that have been genetically characterized. | the in vitro activity of trovafloxacin against 125 strains of mycoplasma species and ureaplasma urealyticum, including fluoroquinolone-susceptible and fluoroquinolone-resistant species, was compared to those of other fluoroquinolones, doxycycline, and erythromycin. the mic at which 90% of isolates are inhibited for all fluoroquinolone-susceptible strains was 0.25 microg/ml. whatever the associated mutations, trovafloxacin exhibited greater activity than the other fluoroquinolones tested against ... | 2000 | 10952617 |
| molecular dissection of mycoplasma hominis. | 2000 | 28670768 | |
| host and pathogen interaction during vaginal infection by trichomonas vaginalis and mycoplasma hominis or ureaplasma urealyticum. | vaginal infections by trichomonas vaginalis and mycoplasma hominis have been shown to be associated. since m. hominis and ureaplasma urealyticum are similar pathogens, both belonging to the class of the mycoplasmata, we describe here a molecular study into the interdependence of u. urealyticum and t. vaginalis during infection. susceptibility towards infection by u. urealyticum depends on genetic polymorphism in the interleukin-1 receptor antagonist (il-1ra) gene. now, we defined the relation be ... | 2001 | 11295198 |
| predictors of the vaginal microflora. | our purpose was to define influences on the patterns of the vaginal microflora. | 2001 | 11303191 |
| the diagnosis of bacterial vaginosis and vaginal flora changes. | in a population of 956 women, attending for contraceptive advice, 131 (13.7%) were found to have bv acording to amsel's criteria. clue cells were detected in 200 (20.9%) women, a positive amine ('sniff') test in 191 (20.0%), a vaginal ph > or = 4.7 in 243 (25.4%) and a "characteristic" vaginal discharge in 104 (10.9%) women. sensitivity, specificity, positive and negative predictive values in relation to bv were calculated for each of these four criteria. the detection of clue cells, an increase ... | 2001 | 11327086 |
| [microbiologic profile in symptomatic pregnant women's genital secretions in gran buenos aires, argentina]. | establish the prevalence of microorganisms associated with genital colonization in symptomatic pregnant women. in order to review the evolution of frequent pathogens ecology and adjust the laboratory design, in a population attended at the public health hospital, in the great buenos aires. | 2001 | 11333586 |
| vaginal microflora associated with bacterial vaginosis in nonpregnant women: reliability of sialidase detection. | to determine the prevalence of gardnerella vaginalis, anaerobic bacteria and mycoplasma hominis in vaginal specimens of women with and without bacterial vaginosis (bv) as well as to determine the sensitivity and specificity of the direct sialidase assay of vaginal fluid as a rapid test for diagnosing this syndrome. | 2001 | 11368254 |
| the detergent octylglucoside neutralizes lipopolysaccharide in a specific manner. | the stimulatory effect of lipopolysaccharide (lps) on human macrophages was found to be neutralized by the detergent octylglucoside (og). both macrophage stimulation and reactivity in a limulus amebocyte lysate test were suppressed by suspension of lps in og at concentrations between 0.25 and 2.5 mm, whereas other stimulatory lipopeptides and lipid containing stimulants were unaffected by og. lps at concentrations causing maximal stimulation of macrophages could be completely neutralized by non- ... | 2001 | 11388610 |
| [genital infection and infertility]. | genital infection is the most important cause of infertility worldwide, affecting not only fallopian tubes but all anatomic urogenital sites, both male and female. | 2001 | 11440663 |
| coupled phase-variable expression and epitope masking of selective surface lipoproteins increase surface phenotypic diversity in mycoplasma hominis. | a new mechanism expanding mycoplasmal surface diversity is described. exposure of surface epitopes on a constitutively expressed membrane protein (p56) of mycoplasma hominis was subject to high-frequency phase variation due to phase-variable expression of the p120 antigen and its selective masking of p56 epitopes. phase-variable masking may confer previously unrealized adaptive capabilities on mycoplasmas. | 2001 | 11447202 |
| the cytosolic hint protein of mycoplasma hominis interacts with two membrane proteins. | histidine triad nucleotide-binding (hint) proteins are dimeric proteins that bind to purines and are found in all three kingdoms: the eukarya, bacteria and archaea. in eukaryotes, hint proteins have been detected intracellularly, but their function is unknown. until now, knowledge about hint proteins in prokaryotes was restricted to sequence similarities and nucleotide-binding studies. in this study, we provide evidence that, in the cell wall-less prokaryote, mycoplasma hominis, the gene encodin ... | 2001 | 11454219 |
| frequency of mycoplasma hominis and ureaplasma urealyticum infections in women with systemic lupus erythematosus. | ureaplasma urealyticum (uu) and mycoplasma hominis (mh) have been detected in the urine of women with systemic lupus erythematosus (sle). we evaluated the presence of these mycoplasma in the endocervix of women presenting sle. a total of 40 sle patients (mean age 40.2 years), and 51 healthy women (mean age 30.9 years), were studied. endocervical swabs were cultured in specific liquid media for mh or uu, detected by a quantitative color assay, and considered positive at >10(3) dilutions. statisti ... | 2001 | 11460209 |
| is mycoplasma hominis a vaginal pathogen? | 2001 | 11463941 | |
| association of mycoplasma genitalium persistence in the urethra with recurrence of nongonococcal urethritis. | most patients with recurrent symptomatic nongonococcal urethritis receive negative test results for chlamydia trachomatis and ureaplasma urealyticum, and the cause of such recurrence usually is unknown. | 2001 | 11473221 |
| phylogeny of the seal mycoplasmas mycoplasma phocae corrig., mycoplasma phocicerebrale corrig. and mycoplasma phocirhinis corrig. based on sequence analysis of 16s rdna. | the nucleotide sequences of the 16s rrna genes from the type strains of three seal mycoplasmas, mycoplasma phocicerebrale, mycoplasma phocae and mycoplasma phocirhinis (formerly mycoplasma phocacerebrale, mycoplasma phocidae and mycoplasma phocarhinis, respectively), were determined by direct dna cycle sequencing. polymorphisms were found in all three 16s rrna gene sequences, showing the existence of two different rrna operons. in m. phocae, a length difference was found between the operons, cau ... | 2001 | 11491337 |
| susceptibilities of mycoplasma hominis, m. pneumoniae, and ureaplasma urealyticum to gar-936, dalfopristin, dirithromycin, evernimicin, gatifloxacin, linezolid, moxifloxacin, quinupristin-dalfopristin, and telithromycin compared to their susceptibilities to reference macrolides, tetracyclines, and quinolones. | the susceptibilities of mycoplasma hominis, mycoplasma pneumoniae, and ureaplasma urealyticum to eight new antimicrobial agents were determined by agar dilution. m. pneumoniae was susceptible to the new glycylcycline gar-936 at 0.12 microg/ml and evernimicin at 4 microg/ml, but it was resistant to linezolid. it was most susceptible to dirithromycin, quinupristin-dalfopristin, telithromycin, reference macrolides, and josamycin. m. hominis was susceptible to linezolid, evernimicin, and gar-936. it ... | 2001 | 11502536 |
| serological investigation of mycoplasma genitalium in infertile women. | the role of mycoplasma genitalium in the pathogenesis of pelvic inflammatory disease has not been characterized. | 2001 | 11527890 |
| two new point mutations at a2062 associated with resistance to 16-membered macrolide antibiotics in mutant strains of mycoplasma hominis. | we describe two mutants of mycoplasma hominis pg-21 which show resistance to 16-membered macrolides but susceptibility to lincosamides, obtained by in vitro exposure to increasing doses of josamycin. the 23s rrna gene showed that each had a mutation (a2062g and a2062t) corresponding to nucleotide 2062 in escherichia coli, which was associated with the acquired phenotype. | 2001 | 11557502 |
| systemic mycoplasma hominis infection in a patient immunocompromised due to combined transplantation of kidney and pancreas. | 2001 | 11561812 | |
| relationship of abnormal vaginal flora, proinflammatory cytokines and idiopathic infertility in women undergoing ivf. | to investigate the prevalence of bacterial vaginosis (bv) and abnormal bacterial vaginal flora in an infertile population and correlate with cervical cytokine production and in vitro fertilization (ivf) outcome. | 2001 | 11584481 |
| genetic heterogeneity of mycoplasma hominis clinical isolates detected during observation of patients with recurrent urogenital inflammation. | a rapid reproducible effective method for molecular typing of mycoplasma hominis strains based on random amplified polymorphic dna (rapd) technique was developed. rapd detected genetic heterogeneity of genomes of mycoplasma hominis clinical isolates and showed changes in the genomes of mycoplasma hominis clinical isolates from patients with chronic infection. | 2001 | 11687848 |
| the p100 coactivator is present in the nuclei of mammary epithelial cells and its abundance is increased in response to prolactin in culture and in mammary tissue during lactation. | the p100 coactivator, first identified as a coactivator of the epstein-barr virus-encoded transcription factor, ebna-2, in cultured cells, interacts with a number of transcription factors. however, the role of p100 in animals is unclear. we found that the abundance of p100 is closely associated with the lactating state in mammary tissue of mice and cows. using two antibodies against independent parts of the protein, p100 immunoreactivity was localised to mammary epithelial cells, and was enriche ... | 2001 | 11691653 |
| effect of controlled expression of the melittin gene on infection caused by mycoplasma hominis in cell culture. | 2001 | 11712175 | |
| molecular design of mycoplasma hominis vaa adhesin. | the variable adherence-associated (vaa) adhesin of the opportunistic human pathogen mycoplasma hominis is a surface-exposed, membrane-associated protein involved in the attachment of the bacterium to host cells. the molecular masses of recombinant 1 and 2 cassette forms of the protein determined by a light-scattering (ls) method were 23.9 kd and 36.5 kd, respectively, and corresponded to their monomeric forms. circular dichroism (cd) spectroscopy of the full-length forms indicated that the vaa p ... | 2001 | 11714926 |
| evaluation of bact/alert system for detection of mycoplasma hominis in simulated blood cultures. | we used simulated blood cultures inoculated with clinical isolates of mycoplasma hominis to determine whether liquid media of the bact/alert (organon teknika, durham, n.c.) will support growth of this fastidious organism and whether its presence can generate a positive signal with the instrument. viability of clinical isolates of m. hominis was maintained for 7 days in bact/alert media, and organisms were able to multiply when 1% gelatin was added to neutralize the mycoplasmastatic effects of th ... | 2001 | 11724840 |