Publications

nutritional factors that affect the production of cholesterol oxidase by rhodococcus equi no. 23.	some nutritional factors affecting the production of cholesterol oxidase (cox) by rhodococcus equi no. 23 were investigated. cholesterol and yeast extract respectively were the best carbon source and nitrogen source for the cox production. the optimum concentration of cholesterol and yeast extract was found to be about 0.1% and 0.4-0.5% (w/v) respectively. in addition, nh4cl, nacl and tween 80 also exhibited enhancing effects on cox production, being maximal at 0.1% (w/v), 0.2% (w/v) and 1.0% (v ...	1997	9428154
detection of orbital implant infection with technetium 99m-labeled leukocytes.	orbital implant infection is a rare cause of anophthalmic socket pain. because of the potential danger of infection spreading to nearby structures, it is of paramount importance to diagnose the condition as soon as possible. scintigraphy is a method for diagnosis of graft infections by radioisotopic imaging of inflammatory sites. we report on a patient with socket pain 3 months after implantation of an acrylic implant. the socket appearance was normal and there were no signs of infection other t ...	1997	9430302
community-acquired lobar pneumonia in an hiv-infected patient.		1997	9433956
development of a rhodococcus equi-escherichia coli plasmid shuttle vector.	isolates of rhodococcus equi from pneumonic foals possess an 85- or 90-kb virulence-associated plasmid. a prominent, thermoregulated surface antigen, vapa, encoded by these plasmids is thought to be important in virulence. a 135-kb fragment containing the origin of replication of r. equi strain 103 virulence-associated plasmid (pots) was identified, sequenced, and its location identified. a simple r. equi-escherichia coli shuttle plasmid (pre-1) derived from the e. coli plasmid pacyc177 and the ...	1997	9435020
identification and epidemiological relationship of rhodococcus equi isolated from cases of lymphadenitis in cattle.	the present study was designed to comparatively investigate 10 rhodococcus equi isolates from cases of lymphadenitis in cattle. the isolates could be identified by cultural and biochemical properties. by serotyping the r. equi isolates 9 and 1, cultures could be classified as nakazawa's serotypes 15 and 8, respectively. the isolates did not agglutinate rabbit erythrocytes, were uniformly susceptible to most of the antibiotics tested, did not contain plasmids nor expressed virulence-associated pr ...	1997	9440194
[emergence of gram-positive organisms: causes, clinical significance and possibilities of control].		1997	9441320
t-cell response during rhodococcus equi infection in a murine experimental model.	rhodococcus equi is a facultative intracellular bacterium that can cause pneumonia in both young horses and immunocompromised humans. in this study, we have tried to determine the t-cell populations that recognize this pathogen during murine infection, as well as the bacterial antigens recognized by these cells. when balb/c mice were hyperimmunized with a virulent r. equi strain, we did not observe preferential expansion of a particular t-cell subset in their spleens. however, when the splenic t ...	1997	9443578
isolation of virulent rhodococcus equi from transtracheal aspirates of foals serodiagnosed by enzyme-linked immunosorbent assay.	although isolation of rhodococcus equi from tracheobronchial aspirates is thought to be a definitive diagnosis of r. equi pneumonia in foals, virulence of isolates from the aspirates of infected foals remains obscure. in the present study, transtracheal aspirates were collected from thirty-one 1- to 6-month-old foals, which showed clinical signs of respiratory tract infection, and r. equi isolates were analyzed for the presence of virulence plasmids and virulence-associated antigens. moreover, t ...	1997	9450238
microbial degradation and treatment of polycyclic aromatic hydrocarbons and plasticizers.	rhodococcus erytropolis and pseudomonas sp. rapidly degrade many kinds of polycyclic aromatic hydrocarbon (pah) compounds such as phenanthrene and phthalate esters such as di(2-ethylhexyl) phthalate, used as plasticizers. these compounds were efficiently removed from wastewater by inoculating viable cells of rhodococcus erythropolis and pseudomonas sp. into activated sludge as a biological treatment system. the rapid pcr method and fluorescent antibody techniques were successfully applied for tr ...	1997	9472317
routine use of pcr-restriction fragment length polymorphism analysis for identification of mycobacteria growing in liquid media.	a pcr-restriction fragment length polymorphism (pcr-rflp) procedure capable of rapidly identifying 28 species of clinically encountered mycobacteria was evaluated for use in the routine identification of acid-fast isolates growing in bactec 12b and 13a liquid media. pcr-rflp identified 100 of 103 acid-fast isolates recovered from 610 patient specimens submitted for culture during the study. the three isolates unidentifiable by pcr-rflp produced restriction patterns not included in the pcr-rflp a ...	1997	8968884
radiologic features of rhodococcus equi pneumonia in aids.	this report outlines the radiological features observed in three cases of rhodococcus equi (r. equi) pneumonia in aids (acquired immunodeficiency syndrome) and reviews another 45 radiological reports published of this emerging opportunistic pneumonia in human immunodeficiency virus (hiv) infected patients. the clinical signs in our three patients consisted in a subacute onset of respiratory symptoms and fever. a low lymphocyte count (< 200 cells/mm3), pulmonary infiltrates, and pleural effusion ...	1997	9056153
bacterial degradation of emulsified crude oil and the effect of various surfactants.	a rhodococcus sp. 094 bacterium was tested for its ability to oxidize alkanes in crude oil emulsified by nonionic chemical and biological surfactants. oxidation rates were measured in a 3-h period by warburg respirometry. 14co2 recovery was measured from the [1-14c]hexadecane spiked crude oil. response to emulsified oil depended on the physiological state of the bacteria (i.e., cells harvested in the exponential and stationary growth phases) were tested. oxidation rates by cells in the exponenti ...	1997	9057292
heterologous expression of biphenyl dioxygenase-encoding genes from a gram-positive broad-spectrum polychlorinated biphenyl degrader and characterization of chlorobiphenyl oxidation by the gene products.	the bpha1a2a3a4 gene cluster, encoding a biphenyl dioxygenase from rhodococcus globerulus p6, a gram-positive microorganism able to degrade a wide spectrum of polychlorobiphenyls (pcbs), was expressed in pseudomonas putida, thereby allowing characterization of chlorobiphenyl oxidation by this enzyme. while p6 biphenyl dioxygenase activity was observed in p. putida containing bpha1a2a3a4, no activity was detected in escherichia coli cells containing the same gene cluster. in e. coli, transcriptio ...	1997	9068637
identification of rhodococcus, gordona and dietzia species using carbon source utilization tests ("biotype-100" strips).	the "biotype-100" identification system (biomérieux, la balme-ies-grottes, france) based on carbon source utilization was evaluated for its ability to discriminate among 10 species of rhodococcus, 7 species of gordona and one species of dietzia. the type strains of three species of tsukamurella and 8 species of nocardia were also included in the study. results were compared with chemotaxonomic and conventional data. carbon source utilization was shown to be reliable, rapid and easy to use when c ...	1997	9765864
interpretation of chest radiographs in aids patients: usefulness of cd4 lymphocyte counts.	specific infections and neoplasms that are complications of acquired immunodeficiency syndrome (aids) occur within various cd4 lymphocyte count ranges. knowledge of how these counts correlate with radiographic appearances of these entities can limit the differential diagnosis because certain conditions are uncommon above a specific count. in patients with cd4 lymphocyte counts above 200 cells/mm3 and radiographic findings of cavitary and noncavitary consolidation, bacterial pneumonia and mycobac ...	1997	9017798
a flavin reductase stimulates dsza and dszc proteins of rhodococcus erythropolis igts8 in vitro.	rhodococcus erythropolis igts8 is a gram positive bacterium, which can catabolize dibenzothiophene to 2-hydroxybiphenyl and inorganic sulfur without the cleavage of carbon-carbon bonds. three structural genes, dsza, dszb, and dszc, have been cloned and shown to be necessary for this phenotype. here, we demonstrate that a fmn:nadph oxidoreductase from vibrio harveyi complements activities of purified dsza and dszc proteins. furthermore, we propose that dsza and dszc are oxygenase units that do no ...	1997	9020064
the plasmid-located haloalkane dehalogenase gene from rhodococcus rhodochrous ncimb 13064.	the haloalkane dehalogenase (dhaa) gene from rhodococcus rhodochrous ncimb 13064 was cloned and sequenced. its comparison with the previously studied dhla gene from xanthobacter autotrophicus gj10 did not show homology. however, the amino acid sequences of the products of these genes showed approximately 30% identity and several of the catalytic amino acid residues were conserved in the ncimb 13,064 dehalogenase. a high level of dhaa expression was demonstrated in escherichia coli cells and this ...	1997	9025284
[photoreactive nitrite hydratase].		1997	9028170
rhodococcus or mycobacterium? an example of misdiagnosis in hiv infection.		1997	9030376
chronic pneumonia caused by rhodococcus equi in a patient without impaired immunity.	a 48 year old, human immunodeficiency virus (hiv)-negative, immunocompetent male patient had a chronic progressive pulmonary infiltrate, without radiological cavitation, in the middle lobe of the right lung produced by rhodococcus equi. he reported direct contact with a diseased dog. the patient was diagnosed by thoracotomy and treated by lobectomy. after 16 months of follow-up, the patient was asymptomatic and had neither recurrence nor immunological disturbances.	1997	9032523
classification of catechol 1,2-dioxygenase family: sequence analysis of a gene for the catechol 1,2-dioxygenase showing high specificity for methylcatechols from gram+ aniline-assimilating rhodococcus erythropolis an-13.	gram+ aniline-assimilating rhodococcus erythropolis an-13 (an-13) produces catechol 1,2-dioxygenase (c12o) showing high enzymatic activities for 3- and 4-methylcatechols [aoki et al. (1984) agric. biol. chem. 48, 2087-2095]. a 3.0 kb sau3ai fragment carrying a gene encoding c12o(cata) was cloned by selection of transformants showing c12o activity from a gene library of an-13. furthermore, we specified a 1.6 kb sali fragment containing cata from the sau3ai fragment by subcloning. sequence analysi ...	1997	9034312
biodegradation of methyl t-butyl ether by pure bacterial cultures.	three pure bacterial cultures degrading methyl t-butyl ether (mtbe) were isolated from activated sludge and fruit of the gingko tree. they have been classified as belonging to the genuses methylobacterium, rhodococcus, and arthrobacter. these cultures degraded 60 ppm mtbe in 1-2 weeks of incubation at 23-25 degrees c. the growth of the isolates on mtbe as sole carbon source is very slow compared with growth on nutrient-rich medium. uniformly-labeled [14c]mtbe was used to determine 14co2 evolutio ...	1997	9035411
identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses.	coryneform bacteria and yeasts of 21 brick cheeses from six german dairies, produced by using undefined ripening cultures, were identified. arthrobacter nicotianae, brevibacterium linens, corynebacterium ammoniagenes, corynebacterium variabilis and rhodococcus fascians were found in significant numbers. out of 148 coryneform isolates 36 could not be identified at the species level. with the exception of a large rennet cheese, the coryneform microflora of rennet and acid cured cheeses were simila ...	1997	9039559
restriction enzyme analysis of the virulence plasmids of vapa-positive rhodococcus equi strains isolated from humans and horses.	restriction enzyme digestion patterns of the large virulence plasmids of 8 human and 37 foal isolates of virulence-associated protein (vapa)-positive rhodococcus equi strains from different sources were compared. foal isolates came from five continents. digestion with ecori divided these plasmids into three closely related types, and digestion with bamhi divided them into three major types which corresponded to the ecori types. the only ecori and bamhi type 3 plasmid was from a single foal isola ...	1997	9041424
location, catalytic activity, and subunit composition of nad-reducing hydrogenases of some alcaligenes strains and rhodococcus opacus mr22	six new strains of alcaligenes enriched for and isolated as nickel-resistant bacteria resemble alcaligenes eutrophus h16 and contain both an nad-reducing, tetrameric soluble hydrogenase and a membrane-bound hydrogenase. none of the soluble hydrogenases share with the rhodococcus opacus mr11 enzyme tetramer the property of being cleaved easily into two dimeric moieties [a hydrogenase (betadelta) and an nadh:acceptor oxidoreductase (alphagamma)], in the absence of nickel or at low ionic strength. ...	1997	9042758
rhodococcus equi pneumonia in patients infected with the human immunodeficiency virus. report of 2 cases and review of the literature.	rhodococcus equi is a cause of lung infection in immunosuppressed hosts. since the start of the hiv epidemic, 76 cases of r. equi lung infection (medline 1985-96) affecting this population have been described. we report 2 additional cases and review the clinical data, radiological findings, treatment and outcome of these 78 patients. the mean age of these patients was 33 y; 69 were male. 71 met the criteria for aids (cdc 1993). fever and cough were the presenting complaints in the majority of pa ...	1997	9571730
monooxygenase-like sequence of a rhodococcus equi gene conferring increased resistance to rifampin by inactivating this antibiotic.	a dna clone from rhodococcus equi conferring low-level rifampin resistance through the ability to inactivate this antibiotic via its decomposition was identified. the iri (inactivation of rifampin) gene consisted of an open reading frame of 1,437 bp encoding a 479-amino-acid sequence strongly resembling those of monooxygenases acting upon phenolic compounds or involved in polyketide antibiotic synthesis. when expressed in escherichia coli, the gene conferred resistance to a > 50-micrograms/ml co ...	1997	8980786
characterization of three distinct extradiol dioxygenases involved in mineralization of dibenzofuran by terrabacter sp. strain dpo360.	the dibenzofuran-degrading bacterial strain dpo360 represents a new species of the genus terrabacter together with the previously described dibenzofuran-mineralizing bacterial strain dpo1361 (k.-h. engesser, v. strubel, k. christoglou, p. fischer, and h. g. rast, fems microbiol. lett. 65:205-210, 1989; v. strubel, ph.d. thesis, university of stuttgart, stuttgart, germany, 1991; v. strubel, h. g. rast, w. fietz, h.-j. knackmuss, and k.-h. engesser, fems microbiol. lett. 58:233-238, 1989). two 2,3 ...	1997	8981980
a proposal to reclassify nocardia pinensis blackall et al. as skermania piniformis gen. nov., comb. nov.	the type strain of nocardia pinensis was the subject of chemotaxonomic and 16s ribosomal dna sequencing studies. the resultant nucleotide sequence was aligned with the sequences of representatives of the genera corynebacterium, dietzia, gordona, mycobacterium, nocardia, rhodococcus, and tsukamurella, and phylogenetic trees were generated by using the fitch-margoliash, maximum-parsimony, maximum-likelihood, and neighbor-joining methods. it was evident from the phylogenetic analyses that n. pinens ...	1997	8995814
a phylogenetic analysis of aerobic polychlorinated biphenyl-degrading bacteria.	several bacterial isolates were characterized based on their abilities to degrade specific polychlorinated biphenyls (pcbs) and their 16s rrna gene sequences. the members of one group of bacteria consisting of alcaligenes species, including the pcb-degrading bacterium alcaligenes eutrophus h850, had strong abilities to degrade a broad range of pcbs but not the di-para-chlorine-substituted congeners. the members of another group, which included the pcb-degrading bacterium originally classified as ...	1997	8995824
subunit topology of the rhodococcus proteasome.	the 20s proteasome, isolated from the nocardioform actinomycete rhodococcus erythropolis strain ni86/21, is built from two alpha-type and two beta-type subunits. in order to probe the subunit topology, we have set up an expression system which allows coexpression of the genes encoding the alpha- and beta-subunits in all possible combinations. the four respective constructs obtained yielded fully assembled and proteolytically active proteasomes. biochemical, kinetic and electron microscopy analys ...	1997	9000518
a novel transporter involved in cobalt uptake.	cobalt is an essential component of a low molecular-mass nitrile hydratase (l-nhase) from rhodococcus rhodochrous j1. we have found a new gene, nhlf, in the dna region sandwiched between nhlba encoding l-nhase and amda encoding amidase, which are involved in the degradation of nitriles. the product of nhlf, nhlf, shows a significant sequence similarity with those of hoxn from alcaligenes eutrophus, hupn from bradyrhizobium japonicum, nixa from helicobacter pylori, and ureh from bacillus sp., whi ...	1997	8990157
characterization of catechol catabolic genes from rhodococcus erythropolis 1cp.	the biochemical characterization of the muconate and the chloromuconate cycloisomerases of the chlorophenol-utilizing rhodococcus erythropolis strain 1cp previously indicated that efficient chloromuconate conversion among the gram-positive bacteria might have evolved independently of that among gram-negative bacteria. based on sequences of the n terminus and of tryptic peptides of the muconate cycloisomerase, a fragment of the corresponding gene has now been amplified and used as a probe for the ...	1997	8990288
cloning, analysis, and overexpression of the gene encoding isobutylamine n-hydroxylase from the valanimycin producer, streptomyces viridifaciens.	the flavoprotein isobutylamine n-hydroxylase (ibah) catalyzes the oxidation of isobutylamine to isobutylhydroxylamine, a key step in the biosynthesis of the azoxy antibiotic valanimycin. by using oligonucleotide primers designed from peptide sequence information derived from native ibah, a fragment of the gene (vlmh) encoding ibah was amplified by pcr from a genomic library of the valanimycin-producing organism, streptomyces viridifaciens mg456-hf10. the gene fragment was then employed as a prob ...	1997	8990292
metabolism of naphthalene, 1-naphthol, indene, and indole by rhodococcus sp. strain ncimb 12038.	the regulation of naphthalene and 1-naphthol metabolism in a rhodococcus sp. (ncimb 12038) has been investigated. the microorganism utilizes separate pathways for the degradation of these compounds, and they are regulated independently. naphthalene metabolism was inducible, but not by salicylate, and 1-naphthol metabolism, although constitutive, was also repressed during growth on salicylate. the biochemistry of naphthalene degradation in this strain was otherwise identical to that found in pseu ...	1997	16535479
formation of a chiral hydroxamic acid with an amidase from rhodococcus erythropolis mp50 and subsequent chemical lossen rearrangement to a chiral amine.	the amidase from rhodococcus erythropolis mp50 demonstrated, in the presence of hydroxylamine, acyltransferase activity and catalyzed the formation of hydroxamates from amides and hydroxylamine. the rates of acyltransferase activity of the purified amidase for the substrates acetamide, phenylacetamide, and 2-phenylpropionamide were higher than the corresponding rates for the hydrolysis reactions. with the substrate 2-phenylpropionamide the hydrolysis reaction and the acyltransferase activity wer ...	1997	16535682
biodegradation of paint stripper solvents in a modified gas lift loop bioreactor.	paint stripping wastes generated during the decontamination and decommissioning of former nuclear facilities contain paint stripping organics (dichloromethane, 2-propanol, and methanol) and bulk materials containing paint pigments. it is desirable to degrade the organic residues as part of an integrated chemical-biological treatment system. we have developed a modified gas lift loop bioreactor employing a defined consortium of rhodococcus rhodochrous strain ofs and hyphomicrobium sp. dm-2 that d ...	1997	18636454
specific detection of clavibacter michiganensis subsp. sepedonicus by amplification of three unique dna sequences isolated by subtraction hybridization.	abstract three single-copy, unique dna fragments, designated cms50, cms72, and cms85, were isolated from strain cs3 of clavibacter michiganensis subsp. sepedonicus by subtraction hybridization using driver dna from c. michiganensis subsp. insidiosus, c. michiganensis subsp. michiganensis, and rhodococcus facians. radio-labeled probes made of these fragments and used in southern blot analysis revealed each to be absolutely specific to all north american c. michiganensis subsp. sepedonicus strains ...	1997	18945054
patterns of phenolic compounds in leafy galls of tobacco.	the chemical composition of ethanolic and aqueous extracts from leafy galls produced after infection of nicotiana tabacum l. plants with rhodococcus fascians was drastically changed compared to uninfected controls. chlorogenic acid was abundant both in uninfected and infected plants, but caffeic acid and another cinnamoyl analogue were new in leafy galls. the most pronounced product induced in leafy galls was identified as 7-o-methyl-6-o-beta-d-glucopyranosyl coumarin (7-methyl esculin). this is ...	1997	19343412
rhodococcus equi infection in non-hiv-infected patients. two case reports and review.	objective: to review two recent cases in hiv-negative subjects in the light of literature reports (52 patients without hiv infection till 1994). methods: epidemiology (animal contacts, risk factors, year, country), clinical presentation, diagnostic methods (x-ray, tomography, microbiological techniques), therapeutic approach (antibiotics, surgery) and outcome were evaluated on the basis of clinical literature reports. results: tumors constituted an important predisposing factor and less frequent ...	1997	11864070
secondary prophylaxis of rhodococcus equi pneumonia in hiv infection: breakthrough despite rifampicin/erythromycin suppressive therapy.		1997	11864093
cell-surface hydrophobicity and scum formation of rhodococcus rhodochrous strains with different colonial morphologies.	rhodococcus rhodochrous has been reported to be one of the micro-organisms responsible for the formation of scum which is thick and viscous biological foam in activated sludge plants. the hydrophobicity of mycolic acids present on the cell surface and the long-branched shape of the hyphae have been thought to contribute to the scum formation. cell surface hydrophobicity and scum formation of four r. rhodochrous strains with different colony morphologies were determined, and the results showed th ...	1997	12452595
kinetic studies of biodegradation of insoluble compounds by continuous determination of oxygen consumption.	continuous determination of oxygen consumption by electrolytic respirometry has been experimented as a means to study the biodegradation kinetics of scarcely soluble environmental pollutants. the substrates used were the polycyclic aromatic hydrocarbons (pah), naphthalene, phenanthrene, anthracene, fluoranthene and pyrene. the definition of an appropriate mode of pah supply, either as crystals or more generally as a solution in a water non-miscible solvent, was found essential for yielding repro ...	1997	12455894
[bacteraemia in patients with hiv disease: aetiological spectrum and profile of antimicrobial susceptibility].	a retrospective survey of non-opportunistic bacterial pathogens isolated from blood cultures of patients with hiv disease has been carried out for a 6-year period, and the antibiotic susceptibility of the 748 microorganisms cultured from 682 consecutive patients, has been evaluated. gram-positive organisms significantly prevailed over gram-negative ones, with staphylococcus aureus, coagulase-negative staphylococci, enterococcus faecalis, xantomonas maltophilia, salmonella and pseudomonas sp. as ...	1997	12847308
clinical evaluation of the serodiagnostic value of enzyme-linked immunosorbent assay for rhodococcus equi infection in foals.	an enzyme-linked immunosorbent assay (elisa) for detection of serum igg antibodies against tween 20-extracted antigen of strain atcc 6939 was applied in hidaka, japan to a total of 752 sick foals showing a variety of signs of infectious disease. an optical density (od) value of more than 0.3 was tentatively fixed to be positive on the basis of readings made of healthy horse sera in previous studies. during a 2 year study, 138 of the 752 sick foals showed an od value of 0.3 or higher and were des ...	1997	15338907
the association of erythromycin ethylsuccinate with acute colitis in horses in sweden.	in sweden there are several reports of mares developing acute colitis while their foals were being treated orally for rhodococcus equi pneumonia with the combination of erythromycin and rifampicin. in this study 6 adult horses were given low oral dosages of these antibiotics, singly or in combination. within 3 days post administration of erythromycin, in one case in combination with rifampicin, 2 horses developed severe colitis (one fatal). clostridium difficile was isolated from one of the hors ...	1997	15338913
[bacterial infections in patients infected by hiv].	individuals infected by the human immuno deficiency are more prone to suffering certain bacterial infections in the course of their clinical evolution. the agents involved in these infections are: streptococcus pneumoniae, haemophilus influenzae, pseudomonas aeruginosa, salmonella spp and campylobacter spp, which occasion an increase in morbidity and mortality. with a lower frequency, but with an equal morbidity and mortality, infections have been found caused by rhodococcus equi, nocardia spp a ...	1998	12891392
bacterial composition of the biofilm on the surface of course sediment of the danube: with special reference to the clinically important bacteria.	on monthly intervals and over a period of 14 months (november, 1993-december, 1994) biofilm samples from sediments taken at the szentendre island on the danube were culturel and the isolated organisms were examined macromorphologically and micromorphogically and tested for oxidase and catalase production and their ability to oxidise and ferment glucose. the majority (85%) of the strains isolated were catalase positive. 43% were oxidase positive, 38% were glucose oxidisers and only 19% fermented ...	1998	14666747
[rhodococcus equi pneumonia in aids: a case report]	we describe the first case, in campania, of rhodococcus equi pneumonia in an hiv positive subject. the clinical symptoms resolved after antibiotic therapy. the pulmonary lesion at 10 months of radiological follow up results just partially reduced in its diameters.	1998	12732826
initial reductive reactions in aerobic microbial metabolism of 2,4,6-trinitrotoluene.	because of its high electron deficiency, initial microbial transformations of 2,4,6-trinitrotoluene (tnt) are characterized by reductive rather than oxidation reactions. the reduction of the nitro groups seems to be the dominating mechanism, whereas hydrogenation of the aromatic ring, as described for picric acid, appears to be of minor importance. thus, two bacterial strains enriched with tnt as a sole source of nitrogen under aerobic conditions, a gram-negative strain called tnt-8 and a gram-p ...	1998	16349484
cytokine induction in murine macrophages infected with virulent and avirulent rhodococcus equi.	to look for a possible correlation between the virulence of rhodococcus equi and its cytokine-inducing capacity, we evaluated intracellular survival and measured cytokine induction by mouse macrophages infected with a virulent strain containing an 85-kb plasmid and expressing vapa (103+), its avirulent plasmid-cured derivative (103-), and heat-killed 103+ (hk). after incubation with similar numbers of bacteria, macrophages infected with 103- contained significantly more organisms than those infe ...	1998	9573060
chemically emulsified crude oil as substrate for bacterial oxidation: differences in species response.	four bacterial species were tested for their abilities to oxidize alkanes in crude oil in water emulsions. the emulsions were prepared by nonionic sorbitan ester and polyoxyethylene ether surfactants. the oxidation rates were measured as initial attack on the emulsions by resting cells pregrown in crude oil media. the bacteria responded differently and both positive and negative effects of surfactant amendment were observed. the same surfactant affected various bacteria differently and the respo ...	1998	9575027
cloning of new rhodococcus extradiol dioxygenase genes and study of their distribution in different rhodococcus strains.	four extradiol dioxygenase genes which encode enzymes active against catechol and substituted catechols were cloned from two different rhodococcus strains, and their nucleotide sequences were determined. a catechol 2,3-dioxygenase gene (edoc) was shown to be identical to the previously described ipbc gene from the isopropylbenzene operon of rhodococcus erythropolis. amino acid sequences deduced from the three other genes (edoa, edob and edod) were shown to have various degrees of homology to dif ...	1998	9579069
recalcitrance of 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene to degradation by pure cultures of 1,1-diphenylethylene-degrading aerobic bacteria.	1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (dde) is the peri-chlorinated derivative of 1,1-diphenylethylene (dpe). biodegradation of dde and dpe by bacteria has so far not been shown. pure cultures of aerobic bacteria involved in biodegradation of styrene and polychlorinated biphenyls (pcb) were therefore screened for their ability to degrade or cometabolize dpe and dde. styrene-metabolizing bacteria (rho-dococcus strains s5 and vlb150) grew with dpe as their sole source of carbon and energy. ...	1998	9581297
in vitro susceptibilities of aerobic and facultative non-spore-forming gram-positive bacilli to hmr 3647 (ru 66647) and 14 other antimicrobials.	the comparative in vitro activity of the ketolide hmr 3647 (ru 66647) and those of structurally related macrolide-lincosamide-streptogramin compounds (erythromycin, roxithromycin, azithromycin, clarithromycin, josamycin, lincomycin, pristinamycin, and quinupristin-dalfopristin) as well as those of benzylpenicillin, doxycycline, vancomycin, teicoplanin, levofloxacin, and rifapentine against 247 aerobic and facultative non-spore-forming gram-positive bacilli were determined by an agar dilution met ...	1998	9593121
transfer of plasmid pto1 from escherichia coli to various representatives of the order actinomycetales by intergeneric conjugation.	plasmid pto1 containing the orit fragment from rk2, the escherichia coli replication function from pbr322, and a dna fragment of actinophage phi c31 with the attachment site was transferred from e. coli s17-1 to strains of the genera actinomadura, arthrobacter, micromonospora, nocardia, rhodococcus, and to 16 strains of the genus streptomyces. the frequency of conjugant formation was 1 x 10(-3)-1 x 10(-5) depending on the strain. hybridization experiments demonstrated that plasmid pto1 integrate ...	1998	9595662
colonic polyps and disseminated infection associated with rhodococcus equi in a patient with aids.		1998	9597270
two nearly identical aromatic compound hydrolase genes in a strong polychlorinated biphenyl degrader, rhodococcus sp. strain rha1.	the two 2-hydroxy-6-oxohepta-2,4-dienoate (hohd) hydrolase genes, etbd1 and etbd2, were cloned from a strong polychlorinated biphenyl (pcb) degrader, rhodococcus sp. strain rha1, and their nucleotide sequences were determined. the etbd2 gene was located in the vicinity of bpha gene homologs and encoded an enzyme whose amino-terminal sequence was very similar to the amino-terminal sequence of the hohd hydrolase which was purified from rha1. using the etbd2 gene fragment as a probe, we cloned the ...	1998	9603807
computation of the electrical double layer properties of semipermeable membranes in multicomponent electrolytes	a methodology is presented for calculating of the surface potential, donnan potential, and ion concentration profiles for semipermeable microbial membranes that is valid for an arbitrary electrolyte composition. this model for surface potential, donnan potential, and charge density was applied to recently reported experimental data for gram-positive bacteria, including bacillus brevis, rhodococcus opacus, rhodococcus erythropolis, and corynebacterium species. these calculations show that previou ...	1998	9603855
the terminal structures of linear plasmids from rhodococcus opacus.	the telomers of several linear plasmids of rhodococcus opacus (formerly nocardia opaca) were studied. the plasmids phg201, phg204 and phg205 carry proteins bound to their ends, as shown by gel retardation experiments. a sequence hybridizing with the terminal sequence of phg207, a recombinant linear plasmid consisting of the left part of phg204 and the right part of phg205, which was analysed in a previous study by the authors, could be detected in all linear plasmids of the wild-type r. opacus s ...	1998	9611802
rhodococcus keratitis.		1998	9613392
new shuttle vectors for rhodococcus sp. r312 (formerly brevibacterium sp. r312), a nitrile hydratase producing strain.	two shuttle vectors named prc52 (10.7 kb) and prk52 (12.2 kb) carrying chloramphenicol (cm) and chloramphenicol plus kanamycin (km) resistance genes, respectively, were constructed by fusion of a cryptic plasmid pbl13869 (replicon pbl1, 5.8 kb) from brevibacterium lactofermentum atcc13869 with pbr328 e. coli plasmid. transformation of rhodococcus sp. r312 (formerly brevibacterium sp. r312) protoplasts was realised with an efficiency of 28 transformants per micrograms of dna.	1998	9637010
fever of unknown origin and anemia with rhodococcus equi infection in an immunocompetent patient.		1998	9644030
pulmonary infection caused by rhodococcus equi in a kidney and pancreas transplant recipient: a case report.		1998	9645819
detection of virulent rhodococcus equi in tracheal aspirate samples by polymerase chain reaction for rapid diagnosis of r. equi pneumonia in foals.	polymerase chain reaction (pcr)-based assays were developed to detect virulent rhodococcus equi in transtracheal aspirate samples from sick foals showing respiratory signs. an oligonucleotide primer pair from the sequence of the virulence-associated 15- to 17-kda antigen gene of the virulence plasmid in virulent r. equi was used to amplify a 564 bp region by pcr, and the result was confirmed by southern blot hybridization. no positive reaction was seen in dna from 13 different microorganisms typ ...	1998	9646466
biodegradation of variable-chain-length alkanes at low temperatures by a psychrotrophic rhodococcus sp.	the psychorotrophic rhodococcus sp. strain q15 was examined for its ability to degrade individual n-alkanes and diesel fuel at low temperatures, and its alkane catabolic pathway was investigated by biochemical and genetic techniques. at 0 and 5 degrees c, q15 mineralized the short-chain alkanes dodecane and hexadecane to a greater extent than that observed for the long-chain alkanes octacosane and dotriacontane. q15 utilized a broad range of aliphatics (c10 to c21 alkanes, branched alkanes, and ...	1998	9647833
overexpression of high-molecular-mass nitrile hydratase from rhodococcus rhodochrous j1 in recombinant rhodococcus cells.	high-molecular-mass nitrile hydratase (h-nhase, 530 kda) is a cobalt-containing enzyme produced by rhodococcus rhodochrous j1. for efficient production of h-nhase in r. rhodochrous atcc12674, several plasmids were constructed. the enzyme was produced in the recombinant rhodococcus cells only in the presence of an upstream region (approximately 4 kb) of the h-nhase gene under the control of the promoter for the amidase-nhase gene cluster from rhodococcus sp. n-774. although h-nhase was produced a ...	1998	9650255
the efficiency of different phenol-degrading bacteria and activated sludges in detoxification of phenolic leachates.	phenolic composition, toxicity and biodegradability of three different phenolic leachates/samples was studied. samples a and c were the leachates from the oil-shale industry spent shale dumps at kohtla-järve, estonia. sample b was a laboratory-prepared synthetic mixture of 7 phenolic compounds mimmicking the phenolic composition of the leachate a. toxicity of these 3 samples was analyzed using two photobacterial test (biotox and microtox), daphnia test (daphtoxkit f pulex) and rotifiers' test (r ...	1998	9650267
rhodococcus equi infection of monocytes/macrophages from human immunodeficiency virus (hiv)-infected patients and healthy individuals: evaluation of intracellular killing and nitric oxide production.	monocytes/macrophages from human immunodeficiency virus (hiv)-infected patients had a defect in their ability to kill rhodococeus equi in vitro, as compared with healthy hiv-seronegative individuals. virulent and avirulent r. equi strains isolated from humans and horses showed no significant intracellular replicative differences within both hiv-positive and -negative monocytes/macrophages. infection with r. equi induced the production of nitric oxide (no) by monocytes/macrophages from healthy in ...	1998	9657316
characterization of proteins utilized in the desulfurization of petroleum products by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi/tof/ms) with delayed extraction is utilized in linear, reflected-ion and post-source decay (psd) modes to directly characterize enzymes being developed for use in a petroleum desulfurization process. the dna sequence for the genes isolated from rhodococcus sp. strain igts8 that produce three of the four enzymes under study had been previously reported with a discrepancy in residue assignments for one of the enzym ...	1998	9657867
characterization of the maleylacetate reductase maca of rhodococcus opacus 1cp and evidence for the presence of an isofunctional enzyme.	maleylacetate reductases (ec 1.3.1.32) have been shown to contribute not only to the bacterial catabolism of some usual aromatic compounds like quinol or resorcinol but also to the degradation of aromatic compounds carrying unusual substituents, such as halogen atoms or nitro groups. genes coding for maleylacetate reductases so far have been analyzed mainly in chloroaromatic compound-utilizing proteobacteria, in which they were found to belong to specialized gene clusters for the turnover of chl ...	1998	9657989
involvement of a rhamnolipid-producing strain of pseudomonas aeruginosa in the degradation of polycyclic aromatic hydrocarbons by a bacterial community.	a rhamnolipid-producing strain of pseudomonas aeruginosa gl1 was isolated from a bacterial community growing on a mixture of polycyclic aromatic hydrocarbons (pah) as sole carbon source. strain gl1 did not grow on pah but grew on known degradation metabolites of phenanthrene (o-phthalic acid) and of naphthalene (salicylic acid). in co-culture with a phenanthrene-degrading strain, ps. aeruginosa gl1 accelerated the degradation of phenanthrene. strain gl1 was resistant to toxic amphiphilic compoun ...	1998	9674130
biocatalytic sulfur removal from fuels: applicability for producing low sulfur gasoline.	environmental regulations are driving r&d efforts to produce low sulfur fuels, including diesel fuel and gasoline for motor vehicles. biocatalytic sulfur removal from fuels has potential applicability for producing low sulfur gasoline. microbial biocatalysts have been identified that can biotransform sulfur compounds found in fuels, including ones that selectively remove sulfur from dibenzothiophene heterocyclic compounds. most attention is give to the 4s pathway of rhodococcus, which can remove ...	1998	9675512
purification and characterization of catechol 1,2-dioxygenase from rhodococcus rhodochrous ncimb 13259 and cloning and sequencing of its cata gene.	a method was developed for the purification of catechol 1, 2-dioxygenase from rhodococcus rhodochrous ncimb 13259 that had been grown in the presence of benzyl alcohol. the enzyme has very similar apparent km (1-2 microm) and vmax (13-19 units/mg of protein) values for the intradiol cleavage of catechol, 3-methylcatechol and 4-methylcatechol and it is optimally active at ph9. cross-linking studies indicate that the enzyme is a homodimer. it contains 0.6 atoms of fe per subunit. the enzyme was cr ...	1998	9677336
vapa-negative rhodococcus equi in a dog with necrotizing pyogranulomatous hepatitis, osteomyelitis, and myositis.		1998	9683085
isolation and characterization of a bacterium possessing a novel aldoxime-dehydration activity and nitrile-degrading enzymes.	a bacterial strain capable of utilizing e-pyridine-3-aldoxime as a nitrogen source was isolated from soil after a 4-month acclimation period and was identified as rhodococcus sp. the strain contained a novel aldoxime dehydration activity that catalyzed a stoichiometric dehydration of e-pyridine-3-aldoxime to form 3-cyanopyridine. the enzyme activity was induced by various aldoximes and nitriles. the strain metabolized the aldoxime as follows: e-pyridine-3-aldoxime was dehydrated to form 3-cyanop ...	1998	9683644
a glutathione s-transferase with activity towards cis-1, 2-dichloroepoxyethane is involved in isoprene utilization by rhodococcus sp. strain ad45.	rhodococcus sp. strain ad45 was isolated from an enrichment culture on isoprene (2-methyl-1,3-butadiene). isoprene-grown cells of strain ad45 oxidized isoprene to 3,4-epoxy-3-methyl-1-butene, cis-1, 2-dichloroethene to cis-1,2-dichloroepoxyethane, and trans-1, 2-dichloroethene to trans-1,2-dichloroepoxyethane. isoprene-grown cells also degraded cis-1,2-dichloroepoxyethane and trans-1, 2-dichloroepoxyethane. all organic chlorine was liberated as chloride during degradation of cis-1,2-dichloroepox ...	1998	9687433
acyl transfer activity of an amidase from rhodococcus sp. strain r312: formation of a wide range of hydroxamic acids.	the enantioselective amidase from rhodococcus sp. strain r312 was produced in escherichia coli and was purified in one chromatographic step. this enzyme was shown to catalyze the acyl transfer reaction to hydroxylamine from a wide range of amides. the optimum working ph values were 7 with neutral amides and 8 with alpha-aminoamides. the reaction occurred according to a ping pong bi bi mechanism. the kinetic constants demonstrated that the presence of a hydrophobic moiety in the carbon side chain ...	1998	9687439
degradation of 1,3-dichloropropene by pseudomonas cichorii 170.	the gram-negative bacterium pseudomonas cichorii 170, isolated from soil that was repeatedly treated with the nematocide 1, 3-dichloropropene, could utilize low concentrations of 1, 3-dichloropropene as a sole carbon and energy source. strain 170 was also able to grow on 3-chloroallyl alcohol, 3-chloroacrylic acid, and several 1-halo-n-alkanes. this organism produced at least three different dehalogenases: a hydrolytic haloalkane dehalogenase specific for haloalkanes and two 3-chloroacrylic acid ...	1998	9687453
microcosm enrichment of biphenyl-degrading microbial communities from soils and sediments.	a microcosm enrichment approach was employed to isolate bacteria which are representative of long-term biphenyl-adapted microbial communities. growth of microorganisms was stimulated by incubating soil and sediment samples from polluted and nonpolluted sites with biphenyl crystals. after 6 months, stable population densities between 8 x 10(9) and 2 x 10(11) cfu/ml were established in the microcosms, and a large percentage of the organisms were able to grow on biphenyl-containing minimal medium p ...	1998	9687466
pheno- and genotyping of rhodococcus equi isolated from faeces of healthy horses and cattle.	the present study was designed to comparatively investigate 21 rhodococcus equi isolates from the faeces of clinically healthy horses and cattle. the isolates were identified by cultural and biochemical properties and by pcr analysis. the latter, targeted to the gene coding for the 16s ribosomal rna, revealed a species specific pcr product. the isolates were further characterised by serotyping with two typing systems, by haemagglutination tests and by plasmid and virulence protein profiling. amo ...	1998	9690599
mycobacterial linear plasmids have an invertron-like structure related to other linear replicons in actinomycetes.	the authors previously identified large plasmids in mycobacterium xenopi, m. branderi and m. celatum which appeared to have a linear topology. this study has confirmed the presence of such linear plasmids in mycobacteria, including m. avium, and demonstrated that the ends of these replicons are covalently bound with protein(s), suggesting an invertron-like structure. the termini of one 25 kb plasmid, designated pclp, from m. celatum were cloned and the first 500 bp of each terminus were sequence ...	1998	9695931
sequence analysis of the cupin gene family in synechocystis pcc6803.	the recently described cupin superfamily of proteins includes the germin and germinlike proteins, of which the cereal oxalate oxidase is the best characterized. this superfamily also includes seed storage proteins, in addition to several microbial enzymes and proteins with unknown function. all these proteins are characterized by the conservation of two central motifs, usually containing two or three histidine residues presumed to be involved with metal binding in the catalytic active site. the ...	1998	9697098
multivesiculated macrophages: their implication in fine-needle aspiration cytology of lung mass lesions.	alveolar macrophages are almost invariably present in percutaneous fine-needle aspiration cytology of the lung. they may predominate, appear foamy and finely vesiculated, or may reflect the cellular composition of the lung mass lesion. in a review of 172 cases of "negative for malignant cells" from the percutaneous lung fine-needle aspiration cytology file in an 8-year period at hines va hospital, the vacuolated macrophages were evaluated qualitatively and quantitatively. among the 53 cases (34% ...	1998	9702484
a novel pathway for the metabolism of caffeine by a mixed culture consortium.	a new oxidative pathway for the degradation of caffeine(1,3,7-trimethylxanthine, i) by a mixed culture consisting of strains belonging to the genera klebsiella and rhodococcus is presented. the mixed culture does not initiate degradation by n-demethylation either complete or partial, but instead carries out oxidation at the c-8 position resulting in the formation of 1,3,7-trimethyluric acid (tmu, ii) which further gets degraded to 3,6,8-trimethylallantoin (tma, iii). both tmu and tma are hithert ...	1998	9705852
report of invasive rhodococcus equi infections in taiwan, with an emphasis on the emergence of multidrug-resistant strains.	from november 1995 to october 1997, seven patients with invasive infections due to rhodococcus equi were treated in taiwan. four patients had pulmonary lesions, and one each of the remaining three patients had a recurrent port-a-cath (kabi-pharmacia, north ryde, new south wales, australia)-related bacteremia, a primary bacteremia, and a brain abscess. three patients had underlying hematologic malignancies, and one each of the remaining four patients had diabetes mellitus, waldenström's macroglob ...	1998	9709889
associations between physical examination, laboratory, and radiographic findings and outcome and subsequent racing performance of foals with rhodococcus equi infection: 115 cases (1984-1992).	to determine whether physical examination, laboratory, or radiographic abnormalities in foals with rhodococcus equi infection were associated with survival, ability to race at least once after recovery, or, for foals that survived and went on to race, subsequent racing performance.	1998	9713534
cloning and expression of a gene encoding cyanidase from pseudomonas stutzeri ak61.	the gene coding for cyanidase, which catalyzes the hydrolysis of cyanide to formate and ammonia, was cloned from chromosomal dna of pseudomonas stutzeri ak61 into escherichia coli. the cyanidase gene consisted of an open reading frame of 1004 bp, and it was predicted that cyanidase was composed of 334 amino acids with a calculated molecular mass of 37,518 da. the amino acid sequence of cyanidase showed a 35.1% and 26.4% homology to aliphatic nitrilase from rhodococcus rhodochrous k22 and cyanide ...	1998	9720205
key enzymes for the degradation of benzoate, m- and p-hydroxybenzoate by some members of the order actinomycetales.	a preliminary screening of numerous species of the order actinomycetales, especially of the genera mycobacterium, nocardia, rhodococcus, pseudonocardia, and streptomyces, showed that many of them are able to metabolize benzoate (b) and p-hydroxybenzoate (phb) as indicated by growth and change of color of the ph-indicator of an agar medium. subsequent experiments with liquid cultures which allowed the analysis of substrate utilization by thin layer chromatography confirmed these results. the stud ...	1998	9726125
initial transformations in the biodegradation of benzothiazoles by rhodococcus isolates.	benzothiazole-2-sulfonate (btso3) is one of the side products occurring in 2-mercaptobenzothiazole (mbt) production wastewater. we are the first to isolate an axenic culture capable of btso3 degradation. the isolate was identified as a rhodococcus erythropolis strain and also degraded 2-hydroxybenzothiazole (obt) and benzothiazole (bt), but not mbt, which was found to inhibit the biodegradation of obt, bt, and btso3. in anaerobic resting cell assays, btso3 was transformed into obt in stoichiomet ...	1998	9726870
rhodococcus equi lung abscess complicating evan's syndrome treated with corticosteroid.	we report a case of rhodococcus equi, an unusual pathogen, causing a right upper lobe lung abscess in a patient with evan's syndrome (auto-immune haemolytic anaemia and thrombocytopenia) who was treated with high-dose corticosteroid therapy. the patient was treated successfully with clarithromycin, vancomycin, ciprofloxacin and imipenen which appear to be effective in combination for this unusual condition in which the treatment regimen has been controversial.	1998	9730805
xas characterization of the active sites of novel intradiol ring-cleaving dioxygenases: hydroxyquinol and chlorocatechol dioxygenases.	the intradiol cleaving dioxygenases hydroxyquinol 1,2-dioxygenase (hqi,20) from nocardiodes simplex 3e, chlorocatechol 1,2-dioxygenase (cic1,20) from rhodococcus erythropolis icp, and their anaerobic substrate adducts (hydroxyquinol-hq1,20 and 4-chlorocatechol-cic1,20) have been characterized through x-ray absorption spectroscopy. in both enzymes the iron(iii) is pentacoordinated and the distance distribution inside the fe(iii) first coordination shell is close to that already found in the exten ...	1998	9738933
limonene-1,2-epoxide hydrolase from rhodococcus erythropolis dcl14 belongs to a novel class of epoxide hydrolases.	an epoxide hydrolase from rhodococcus erythropolis dcl14 catalyzes the hydrolysis of limonene-1,2-epoxide to limonene-1,2-diol. the enzyme is induced when r. erythropolis is grown on monoterpenes, reflecting its role in the limonene degradation pathway of this microorganism. limonene-1,2-epoxide hydrolase was purified to homogeneity. it is a monomeric cytoplasmic enzyme of 17 kda, and its n-terminal amino acid sequence was determined. no cofactor was required for activity of this colorless enzym ...	1998	9748436
[development of microbiological technology of air deodoration in laboratory-industrial conditions using a pilot plant].	laboratory tests were performed to select a complex of bacterial strains capable of effective deodoration of waste air produced by an animal formulated feed works under elevated temperature with the presence of numerous organic pollutants. the complex included species from the general nocardia, rhodococcus, and comamonas. the biocatalyst was tested in a real industrial process with the use of a pilot plant for microbiological deodoration of waste air. the test lasted for over six months and conf ...	1998	9749432
the genus rhodococcus.		1998	9750292
detection of a nitric oxide synthase possibly involved in the regulation of the rhodococcus sp r312 nitrile hydratase.	crude homogenates from rhodococcus sp 312 catalyze the conversion of l-arginine into l-citrulline and no2-, the usual oxidation product of no under aerobic conditions. they also catalyze the conversion of n omega-hydroxy-l-arginine (noha) into l-citrulline and no2- with similar rates (10-15 and 100-150 nmol of product.min-1.(mg of protein)-1 respectively for the crude homogenate and for a fraction obtained from ammonium sulfate precipitation). l-citrulline formation is strongly inhibited by clas ...	1998	9753635
[bacteriological and clinical aspects of corynebacterium].	the microbiologists use the term corynebacteria to describe aerobically growing, asporogenous, irregularly sharped gram-positive rods. they comprise strictly aerobic bacteria isolated from environment as well as preferentially anaerobic bacteria found in clinical specimens. a large part of these bacteria is considered as commensal of skin and mucous membranes. this group of organisms has recently been subjected to considerable taxonomic revisions, which have resulted in the proposal of several n ...	1998	9754261
[cerebral abscess caused by rhodococcus equi in an immunocompetent patient].		1998	9763754
the 20s proteasome of streptomyces coelicolor.	20s proteasomes were purified from streptomyces coelicolor a3(2) and shown to be built from one alpha-type subunit (prca) and one beta-type subunit (prcb). the enzyme displayed chymotrypsin-like activity on synthetic substrates and was sensitive to peptide aldehyde and peptide vinyl sulfone inhibitors and to the streptomyces metabolite lactacystin. characterization of the structural genes revealed an operon-like gene organization (prcba) similar to rhodococcus and mycobacterium spp. and showed t ...	1998	9765579
[characterization of aldehyde dehydrogenase gene fragment from mung bean vigna radiata using the polymerase chain reaction].	two degenerate oligonucleotide sequence primers and polymerase chain reactions on total dna have been utilized to clone on 651--bp gene fragment coding the central part of amino acid sequence of an earlier unknown aldehyde dehydrogenase (aldh) from mung bean. the deduced partial amino acid sequence for this aldehyde dehydrogenase shows about 65% sequence identity to aldhs of vibrio cholerae rhodococcus sp., alcaligenes eutrophus and about 45% sequence identity to mammalian aldhs 1 and 2, aldhs o ...	1998	9778740