Publications

atypical invasive external otitis from aspergillus.	we report a rare case of invasive external otitis caused by aspergillosis in an elderly nondiabetic patient. amphotericin b therapy was curative. atypical features of the presentation delayed diagnosis. early use of tissue biopsy and culture to guide prompt initiation of therapy is recommended. the clinical spectrum and microbiology of invasive aspergillosis are also reviewed.	1988	3408569
atypical invasive external otitis from aspergillus.	we report a rare case of invasive external otitis caused by aspergillosis in an elderly nondiabetic patient. amphotericin b therapy was curative. atypical features of the presentation delayed diagnosis. early use of tissue biopsy and culture to guide prompt initiation of therapy is recommended. the clinical spectrum and microbiology of invasive aspergillosis are also reviewed.	1988	3408569
[immunomodulating properties of khlotazol].	a nonsteroidal anti-inflammatory agent khlotazol administered to rats in doses of 40-80 mg/kg for 5 days stimulated synthesis of igm- and igg-antibodies to ram erythrocytes and increased the number of antibody-forming cells (afc) and rosette-forming cells (rfc) in the spleen. when the doses were increased to 160-320 mg/kg, the stimulating effect of the drug on the production of hemagglutinins retained but dose-dependent inhibition of the number of afc and rfc was found. when administered in a do ...	1988	3410037
influence of increasing concentrations of ethanol on food and water intake, body weight, and wheel-running of male sprague-dawley rats.	male sprague-dawley rats were used to study the influence of increasing concentrations of ethanol on wheel-running, food and water intake and body weight. animals were housed individually in screen-bottom cages (n = 12) or in activity wheel cages (n = 12). half the animals in each activity condition received an ethanol-free liquid diet, and the remainder received liquid diet in which the concentration of ethanol was increased by 5% of energy intake per week to a maximum of 35%, at the expense of ...	1988	3413194
a hyperthermic syndrome in two subjects with acute hydrocephalus.	although intracranial hypertension may cause autonomic disturbances, as well as alterations in the regulation of body temperature, an acute hyperthermic syndrome with autonomic disturbance as a consequence of hydrocephalus has not been described previously. two subjects presented with such a syndrome, with each of several episodes of acute shunt failure and hydrocephalus. with correction of the hydrocephalus, the autonomic disturbances and fever immediately cleared. observations from human and e ...	1988	3415522
[pharmacological correction of experimental alcoholic embryopathy].	in the experiments on the progeny of ethanol-exposed rats it was shown that consumption of 15% alcohol instead of water during pregnancy resulted in the worsening of shuttlebox avoidance learning and decrease in succinate dehydrogenase activity in the visual and sensorimotor cortex. these data are indicative of cerebral hypoxia during embryogenesis. the injection of synthetic opioid peptide dalargin during critical periods of development (at the end of embryogenesis and early ontogeny) prevented ...	1988	3416055
influence of coenzyme q10 on doxorubicin uptake and metabolism by mouse myocardial cells in culture.		1988	3416371
quantification and characterization of enkephalins in the upper part of the cat digestive tract and the coeliac ganglia.	the [met]enkephalin, [leu]enkephalin and [met]enkephalin-arg-gly-leu contents of the upper part of the digestive tract (lower oesophageal sphincter, fundus, antrum, pylorus, duodenum, ileum) and coeliac ganglia of the cat were determined and identified. the enkephalin content of all the structures studied, expressed in femtomole/mg of wet tissue, was found to range from 83 to 446 with [met]enkephalin; 19 to 63 with [leu]enkephalin; 2.5 to 13 with [met]enkephalin-arg-gly-leu. in the muscular and ...	1988	3419585
common genetic pools between human populations.	a further inquiry on modern human origins, based on common genetic pool surveys of rigorously selected population samples and highly informative immunological polymorphisms, provides new evidence of an occidental-oriental population split as the origin of human gene pool divergences. the most likely ancestral genetic profile is discussed in the context of the debate raised by preliminary dna restriction fragment length polymorphism studies, which contradict the conclusions drawn from classical b ...	1988	3422217
new attachment formation following controlled tissue regeneration using biodegradable membranes.	the present study evaluated the potential for reestablishment of connective tissue attachment after exclusion of the gingival connective tissue and epithelium during healing after periodontal surgery in a dog model. buccal mucoperiosteal flaps were elevated in all four premolar regions in two dogs. the buccal bone was reduced to approximately 25% of its original level on 24 roots. before the elevated flaps were replaced and sutured, membranes were adjusted to cover the exposed root surfaces. on ...	1988	3422287
an in vitro model to study bacterial invasion of periodontal tissues.	in periodontal disease, the abilities of bacteria to adhere to and degrade in vivo basement membranes should be considered as two of the rate-limiting steps for the potential active or passive invasion of gingival connective tissues. to study these mechanisms in greater detail, we used the pf hr-9 basement-membrane-like matrix to establish an in vitro model of bacterial invasion and degradation. three gram-negative anaerobic periodontopathic organisms, bacteroides gingivalis, fusobacterium nucle ...	1988	3422291
refractory periodontitis: mixed infection with bacteroides gingivalis and other unusual bacteroides species. a case report.	host immune response and the predominant subgingival microflora were evaluated in a 47-year-old male exhibiting severe, recurrent periodontitis. the patient's neutrophils were chemotactically elevated but other functions were within normal limits. significantly, bacteroides gingivalis and bacteroides zoogleoformans constituted 80% of the cultivable microflora and total cell count in subgingival plaque. the remainder of the cultivable microbiota was comprised of fusobacterium nucleatum and haemop ...	1988	3162983
[primary myelofibrosis showing megakaryoblastic crisis: a case report].		1988	3164424
[in vitro organ cultures of human embryonic and fetal thymus].		1988	3168902
characteristics of glutamate dehydrogenase, a new diagnostic marker for the genus fusobacterium.	enzymes representative of carbohydrate and nitrogen metabolism were screened for their presence and activity amongst species of the genus fusobacterium. glutamate dehydrogenase (gdh) was reliably detected in all 25 strains studied. the ph profile of this enzyme and the dna base composition of selected strains were also determined. dna base composition of selected strains ranged between 28-32.9 mol% g + c. gdh was active between ph 7.5-11.5 but two ph profiles of activity, with optima at 9.5 and ...	1988	3171543
effect of mycoplasmas on natural cytotoxic activity and release of tumor necrosis factor alpha by spleen cells.	it has been reported that mycoplasma-infected cells are more sensitive to lysis by natural cytotoxic (nc) effector cells and that splenic nc cells release tumor necrosis factor (tnf-alpha) when they lyse sensitive target cells. here we showed that spleen cells released tnf-alpha when they were incubated with nc-sensitive cells that were infected with mycoplasmas or when they were incubated with mycoplasmas alone, but did not release tnf-alpha when incubated with nc-sensitive cells that were not ...	1988	3182071
tetracycline resistance and tetm in oral anaerobic bacteria and neisseria perflava-n. sicca.	tetracycline-resistant organisms isolated from six patients with periodontal disease included bacteroides spp., eubacterium spp., fusobacterium nucleatum, neisseria perflava-n. sicca, peptostreptococcus anaerobius, veillonella parvula, and facultative streptococci. all but the bacteroides spp. and eubacterium spp. hybridized with the tetm determinant. an additional 417 bacterial strains were screened, and 4% of both the oral streptococci and the fusobacterium spp. hybridized with the tetm probe.	1988	3190205
[analysis of the hypothermic action of etimizol].	in chronic experiments on rabbits it was shown that the hypothermic effect appears in the series of antipheins after the addition of ethyl radical in the first position of imidazole ring. the hypothermic effect of etimizol is more pronounced in the brain than in the body, whereas caffeine equally increases the temperature of the brain and body.	1988	3191969
[comparative study of the progestagenic action of 16 alpha, 17 alpha-cyclohexanoprogesterones (pregna-d'6-pentarans) when administered by different routes].	a comparative study of the progestational activity of modified pentarans depending on the dose and the route of administration was carried out. it was shown that modification of the molecule of 16 alpha, 17 alpha-cyclohexanoprogesterones by introducing and additional double bond in positions 6, 7 and methyl group in position 6 provided a high gestational effect, being particularly pronounced at intramuscular administration.	1988	3191979
cauda equina syndrome secondary to disseminated zygomycosis.	we describe a case of cauda equina syndrome secondary to disseminated zygomycosis. a 52-year-old man had myelodysplastic syndrome, progressive weakness of the lower extremities, and incontinence. neuroradiological findings were consistent with lumbar stenosis and probable disk herniation. a lung lesion was also discovered on the chest roentgenogram. the patient's condition rapidly deteriorated after surgical decompression. attempts at determining other etiologies were unsuccessful. at autopsy, d ...	1988	3193592
functional and structural alterations of the nervous system induced by avian retrovirus rav-7.	chickens infected as embryos with rav-7 developed neurological signs including ataxia, lethargy, and imbalance. evoked spinal cord potentials for rav-7 infected sc chickens were considerably slower (64.8 m/s) than for uninfected sc (103.4 m/s), genetically hypothyroid (os) (93.9 m/s) or special c (95.1 m/s) chickens. conduction velocity measurements of sciatic nerves showed normal values for all the chickens examined in this study. histopathological studies revealed non-suppurative meningoenceph ...	1988	3193874
functional and structural alterations of the nervous system induced by avian retrovirus rav-7.	chickens infected as embryos with rav-7 developed neurological signs including ataxia, lethargy, and imbalance. evoked spinal cord potentials for rav-7 infected sc chickens were considerably slower (64.8 m/s) than for uninfected sc (103.4 m/s), genetically hypothyroid (os) (93.9 m/s) or special c (95.1 m/s) chickens. conduction velocity measurements of sciatic nerves showed normal values for all the chickens examined in this study. histopathological studies revealed non-suppurative meningoenceph ...	1988	3193874
the comparative cytotoxicity of periodontal bacteria.	the direct cytotoxicity of sonic extracts (se) from nine periodontal bacteria for human gingival fibroblasts (hgf) was compared. equivalent dosages (in terms of protein concentration) of se were used to challenge hgf cultures. the cytotoxic potential of each se was assessed by its ability to (1) inhibit hgf proliferation, as measured by direct cell counts; (2) inhibit 3h-thymidine incorporation in hgf cultures; or (3) cause morphological alterations of the cells in challenged cultures. the highe ...	1988	3204481
evolution of influenza polymerase: nucleotide sequence of the pb2 gene of a/chile/1/83 (h1 n1).	the complete nucleotide sequence of the pb2 gene of influenza virus a/chile/1/83 (h1 n1) is presented. sequence comparison between a/chile pb2 protein and the known pb2 sequences of the influenza strains a/wsn/33 (h1 n1), a/pr/8/34 (h1 n1), a/nt/60/68 (h3 n2), a/kiev/59/79 (h1 n1), a/fpv/rostock/34 (h7 n1), and b/ann arbor/1/66 indicates extensive amino acid homology for the influenza a virus pb2 proteins. small clusters of basic amino acids are conserved in all pb2 proteins including the influe ...	1988	3214273
outer membrane protein pattern of eubacterium plautii.	the outer membrane sds-page pattern of eubacterium plautii was characterized by a large number of surface exposed low- and high-molecular-mass proteins. silver stainable carbohydrate was not present. the pattern was clearly distinct from those of outer membrane preparations of eubacterium saburreum and fusobacterium nucleatum. the results are compatible with a gram-positive cell wall structure in e. plautii.	1988	3221197
[nursing research based on human engineering: the home environment].		1988	3221700
in vitro activity of pefloxacin compared with five other quinolones on nalidixic acid-resistant proteae species.	the in vitro activity of pefloxacin, a new fluoroquinolone, was compared with that of 5 other quinolone compounds (nalidixic and pipemidic acids, norfloxacin, ciprofloxacin, and ofloxacin) against 416 strains of proteae spp. isolated from urine specimens of hospitalized patients with acute urinary tract infections (uti). ciprofloxacin was the most active agent. norfloxacin, ofloxacin, and pefloxacin were similarly active against proteus strains (mic90 = 0.39 microgram/ml). against providencia sp ...	1988	3224396
a combined tension band and lag screw technique for fixation of olecranon osteotomies.	a combined tension band and lag screw technique for fixation of olecranon osteotomies was used in six canine clinical patients weighing 4.5 to 19 kg. after the proximal part of the ulnar shaft was exposed, a screw hole was drilled and tapped just cranial to the caudal cortex of the olecranon. an osteotomy was performed and the hole in the olecranon fragment was overdrilled to form a gliding hole. for reconstruction, the olecranon was reduced anatomically and compressed with a screw placed in lag ...	1988	3232327
a combined tension band and lag screw technique for fixation of olecranon osteotomies.	a combined tension band and lag screw technique for fixation of olecranon osteotomies was used in six canine clinical patients weighing 4.5 to 19 kg. after the proximal part of the ulnar shaft was exposed, a screw hole was drilled and tapped just cranial to the caudal cortex of the olecranon. an osteotomy was performed and the hole in the olecranon fragment was overdrilled to form a gliding hole. for reconstruction, the olecranon was reduced anatomically and compressed with a screw placed in lag ...	1988	3232327
effects of progressively increased doses of theophylline and of s 9795 on hemodynamics, blood gases and lung mechanics in dogs.	we investigated the effects of progressively increased i.v. doses of theophylline and of the newly introduced methylxanthine derivative s 9795 on hemodynamics, blood gases and lung mechanics in 2 groups each of 8 pentobarbital sodium-anesthetized dogs. the maximum plasma levels that were attained were 56.6 +/- 3.4 micrograms/ml (mean +/- s.e.) for theophylline and 12 +/- 1.2 micrograms/ml for s 9795. theophylline increased heart rate and cardiac output, decreased arterial po2, increased o2 consu ...	1988	3233049
lack of correlation between anatomic infarct size and ventriculographic variables following coronary occlusion in closed-chest dogs.	1. although the effects of therapeutic interventions upon infarct size are frequently assessed on the basis of left ventricular ejection fraction and segmental contraction, the correlation of these variables with infarct size has not been thoroughly evaluated. to explore such relationships, we occluded the left anterior descending coronary artery of 22 closed-chest dogs and performed contrast ventriculography one week later. regional myocardial function was evaluated by a computer system in 60 r ...	1988	3248241
[acid soluble glycoprotein in human saliva with monoclonal antibody].		1988	3253589
ph changes during simultaneous metabolism of urea and carbohydrate by human salivary bacteria in vitro.	the effect of the wide natural variation in oral ureolysis rates on the ph changes resulting from simultaneous metabolism of 25 mm urea and 2.8 mm glucose in salivary-sediment bacteria were investigated. the ph curves were complex, and included distinctive plateaux indicative of balanced acid and base production. these neutralization plateaux occurred at different phs, which were a function (r2 = 0.98) of the ureolytic rate as measured by the log of the initial ph-change rate in the urea-only re ...	1988	3254127
evidence for the existence of two classes of corncob (coaggregation) receptor in fusobacterium nucleatum.		1988	3254470
production of mouse monoclonal antibodies for the analysis of idiotypes in serum of patients with chronic lymphatic leukaemia.	in this report a simple procedure for the production of murine monoclonal antibodies (moab) against the idiotype of malignant b cells is described. mice were immunized with lymphoid cells from patients with b-cell chronic lymphocytic leukaemia (b-cll). after fusion of the spleen cells, hybridoma supernatants were screened for anti-idiotypic moab in elisa with immunoglobulins obtained from tumour-cell lysates, xenohybridomas and patients' sera. the anti-idiotypic moab were used to study tumour ce ...	1988	3257881
the function of human intercellular adhesion molecule-1 (icam-1) in the generation of an immune response.	monoclonal antibody rr 1/1 directed against the putative lfa-1 ligand molecule intracellular adhesion molecule-1 (icam-1) was found to inhibit the t cell proliferative response to the antigen ppd. interestingly, the percentage of unstimulated monocytes which expressed icam-1 on their surface appeared to vary greatly from person to person although the majority of monocytes did express high levels of icam-1 within their cytoplasm and surface expression could be rapidly induced on most cells by adh ...	1988	3257922
antibody synthesis specific for nonoral antigens in inflamed gingiva.	in vitro experimentation indicates that periodontitis-associated bacteria contain potent polyclonal b-cell activators (pba). we reasoned that if pba were operative in vivo, plasma cells specific for nonoral antigens should be present in the inflamed gingival tissues, which are characterized by a plasma cell infiltrate. to test this, rabbits with experimental periodontitis were immunized in the hind legs with the histochemically detectable antigen horseradish peroxidase (hrp) or glucose oxidase ( ...	1988	3257940
alloimmune b-lymphocytes modify the myocardium contractility-inducing release of srs-a.	it has been previously demonstrated that murine alloimmune lymphoid cells were able to exert positive or negative inotropic effects on isolated mouse atria depending on the cellular type used. here we show that balb/c anti-c3h b-cells induced positive inotropic action on c3h mouse atria. supernatants of alloimmunized b-cells co-cultivated with c3h myocardium exerted the same biological effect as alloimmune b-cells, indicating that a soluble factor is involved. inhibitors of lipoxygenase(s) of ar ...	1988	3263338
a second chain of human cd8 is expressed on peripheral blood lymphocytes.	human cd8 has been thought to consist of disulfide-linked homodimers and homomultimers of a single polypeptide chain homologous to mouse and rat cd8 alpha. in contrast, mouse and rat cd8 are composed of disulfide-linked heterodimers of alpha and beta chains. we have now isolated and sequenced cdna clones encoding a human homologue of mouse and rat cd8 beta. one such clone was inserted into an expression vector and its encoded product was shown to be expressed on the cell surface after cotransfec ...	1988	3264320
expression of a functional cd3-ti antigen/mhc receptor in the absence of surface cd2. analysis with clonal jurkat cell mutants.	to investigate the requirement for cd2 expression in activation of t lymphocytes via the cd3-ti antigen/mhc receptor complex, we produced and characterized a series of cd2- jurkat variants. these mutants lack detectable surface cd2 as determined by indirect immunofluorescence, immunoprecipitation analysis, and specific radiolabeled antibody binding assay, but nevertheless, expressed normal numbers of cd3-ti receptors. as expected, the combination of anti-cd2 antibodies, termed anti-t112 and anti ...	1988	3264323
immunobiological properties of lipopolysaccharides isolated from fusobacterium nucleatum and f. necrophorum.	lipopolysaccharides (lpss) were isolated from fusobacterium nucleatum atcc 10953 and f. necrophorum atcc 25286 by the hot phenol/water procedure. f. nucleatum lps was composed of 16% (w/w) carbohydrate, 10% (w/w) hexosamine and 40% (w/w) fatty acid, while f. necrophorum lps was composed of 26% (w/w) carbohydrate, 12% (w/w) hexosamine and 28% (w/w) fatty acid. these lps preparations induced mitogenic responses in spleen cells of balb/c, balb/c (nu/nu) and c3h/hen mice, and these responses were su ...	1988	3265430
oscillations and chaos in epidemics: a nonlinear dynamic study of six childhood diseases in copenhagen, denmark.	using traditional spectral analysis and recently developed non-linear methods, we analyze the incidence of six childhood diseases in copenhagen, denmark. in three cases, measles, mumps, rubella, the dynamics suggest low dimensional chaos. outbreaks of chicken pox, on the other hand, conform to an annual cycle with noise superimposed. the remaining diseases, pertussis and scarlet fever, remain problematic. the real epidemics are compared with the output of a monte carlo analog of the seir model f ...	1988	3266037
virus-specific memory t cells are pgp-1+ and can be selectively activated with phorbol ester and calcium ionophore.	memory lymphocytic choriomeningitis virus (lcmv)-immune cytotoxic t-lymphocyte precursors (ctlp) can be stimulated to proliferate and to mediate specific cytotoxic activity following incubation with phorbol myristate acetate (pma), calcium ionophore (cai), and interleukin 2 (il-2). this protocol can be used to selectively induced virus-specific ctl activity under both bulk culture and limiting dilution conditions, in the absence of added antigen. there is no concurrent stimulation of alloreactiv ...	1988	3282677
on the prevention and treatment of calcification disorders of old age.	from several investigations it is known that magnesium oxide or hydroxide therapy causes a considerable delay of kidney stone recurrence in man. further, it is known that the mortality of populations due to cardiovascular disease in areas with hard drinking water containing more magnesium and having a higher mg/ca ratio than soft water, is considerably lower than in soft water areas. in the present study a physiological model for the homeostasis of calcium and phosphate is given. it is shown tha ...	1988	3285142
inhibitory effect of gnrh on isolated rat uterine muscle contractility.	the effect of gnrh upon uterine contractions of both non-pregnant and pregnant rats was examined in vitro. in the non-pregnant rat uterus, gnrh inhibited in a concentration-and-time dependent manner the contractions induced by acetylcholine and oxytocin, but not those caused by bradykinin and angiotensin ii. gnrh also inhibited the rhythmic contractions induced by oxytocin in uterine strips from late pregnant rats. these findings show that gnrh has a direct inhibitory effect on the rat uterine c ...	1988	3290605
effect of aldose reductase inhibition on nerve conduction velocity and resistance to ischemic conduction block in experimental diabetes.	the relationships between nerve polyol levels and both nerve conduction velocity (ncv) and resistance to ischemic conduction block (ricb) in streptozocin-induced diabetic rats were examined in two studies. in the first study, sciatic ncv and ricb of the tail nerve, assessed by measuring the time to disappearance of the nerve action potential after the tail was rendered ischemic, were measured in nondiabetic rats, untreated diabetic rats, and diabetic rats given statil, an aldose reductase inhibi ...	1988	3133261
synthetic aci-reductones: 3,4-dihydroxy-2h-1-benzopyran-2-ones and their cis- and trans-4a,5,6,7,8,8a-hexahydro diastereomers. antiaggregatory, antilipidemic, and redox properties compared to those of the 4-substituted 2-hydroxytetronic acids.	synthetic procedures for the elaboration of aci-reductones belonging to the 6- or 7-mono- or bis-substituted-3,4-dihydroxy-2h-1-benzopyran-2-ones (6-10) and their cis- and trans-4a,5,6,7,8,8a-hexahydro diastereomers (11, 12) are described. hexahydrobenzopyranone aci-reductones were conveniently prepared by using meldrum's synthon (2,2-dimethyl-1,3-dioxane-4,6-dione, 49). certain of these substances were evaluated for antilipidemic activity in the cholesterol-fed rat model, and all analogues were ...	1988	3133477
the effect of nicotine on human endothelial cell release of prostaglandins and ultrastructure.	the acute and sub-acute effects of nicotine at concentrations between 10(-9)m and 10(-2)m on human umbilical vein endothelial cell release of prostacyclin and prostaglandin e2 and the sub-acute effect on the endothelial cell ultrastructure have been examined. the acute effect of nicotine on prostaglandin release has been assessed by measuring release of prostacyclin and prostaglandin e2 following stimulation of confluent monolayers of endothelial cells with a23187 in the presence of nicotine. th ...	1988	3134041
influence of radiant heat stress on respiratory water loss in new-born lambs.	respiratory water loss, oxygen consumption and carbon dioxide production were measured during rest and during moderate radiant heat stress in 9 healthy unanaesthetized lambs, age 4-15 days. the method used is based on an open flow-through system with a mass spectrometer for gas analyses. respiratory water loss was 12.7 mg/kg min when the lamb was at rest and increased to 24.7 mg/kg min during heat stress. oxygen consumption was 13.9 ml/kg min at rest and remained unchanged during heat stress. th ...	1988	3134055
analysis of phorbol ester stimulated human megakaryocyte development.	megakaryocytes are relatively rare components of human bone marrow, making the study of their maturation difficult. phorbol esters can act as differentiating agents in a number of cell systems including murine megakaryocytes. we report the effects of phorbol esters on the previously described long-term human megakaryocytic leukemia cell culture, est-iu. while two nontransforming phorbols fail to affect these cells, the transforming phorbol 12-o-tetradecanoylphorbol-13-acetate (tpa) induces a phe ...	1988	3134065
analysis of phorbol ester stimulated human megakaryocyte development.	megakaryocytes are relatively rare components of human bone marrow, making the study of their maturation difficult. phorbol esters can act as differentiating agents in a number of cell systems including murine megakaryocytes. we report the effects of phorbol esters on the previously described long-term human megakaryocytic leukemia cell culture, est-iu. while two nontransforming phorbols fail to affect these cells, the transforming phorbol 12-o-tetradecanoylphorbol-13-acetate (tpa) induces a phe ...	1988	3134065
comparative studies on the low density lipoprotein subfractions from pig and man.	(1) by means of a single spin density gradient ultracentrifugation method, sera from six human volunteers and six pigs were studied; three or four distinct low density lipoprotein (ldl) subfractions could be seen in the pig serum (ldl-1a, ldl-1, ldl-2 and ldl-3) and three in the human serum (ldl-1a, ldl-1b and ldl-2). the subdivision of ldl into subfractions was less clear for the human subjects than for the pigs. (2) physicochemical characteristics (chemical composition, apoprotein content, siz ...	1988	3136968
[results of growth hormone treatment in slow-growing children with normal plasma growth hormone levels in provocation tests].		1988	3137696
enzymatic capacity and protein synthesis in human muscle postoperatively.	the glycolytic and aerobic oxidative capacity in skeletal muscle was investigated to reveal if the decrease seen in muscle protein synthesis is accompanied by a fall in the enzymatic capacity to oxidize substrates. six patients undergoing elective abdominal surgery were investigated by percutaneous muscle biopsies taken before surgery and on the first and third postoperative days. protein synthesis as assessed by the polyribosome concentration was 40% lower on the third day postoperatively than ...	1988	3144105
[effect of eicosanoids on the accumulation of cholesterol and the proliferation of subendothelial cells in the human aorta].	the effect of prostacyclin and stable thromboxane analog a2 on endothelial culture of human aorta was studied. it was shown that prostacyclin inhibited accumulation of cholesterol in the cells and their proliferation, while thromboxane exhibited an opposite effect. calcium antagonists potentiated effects of prostacyclin and inhibited them in respect to thromboxane. screening of a number of synthetic agents affecting arachidonic acid metabolism was carried out. it was found that lipoxygenase inhi ...	1988	3122858
production of immunoreactive growth hormone by mononuclear leukocytes.	in the present study, we evaluated whether mononuclear leukocytes could synthesize and secrete growth hormone (gh) in vitro. by using rna slot blot analysis, we detected maximum spontaneous levels of specific gh mrna in the cytoplasm of rat leukocytes after a 4-h incubation. northern gel analysis demonstrated that the specific leukocyte gh rna was polyadenylated and had a molecular mass of 1.0 kb. further studies using immunofluorescence, antibody affinity chromatography, and sephacryl gel filtr ...	1988	3044906
[mass-spectrometric determination of specificity of proteinases].	analysis of methods of the proteinase substrate specificity determination revealed that michaelis constants found from the rates of hydrolysis of small synthetic substrates cannot be a good measure of selectivity, especially for proteinases of broad specificity used for hydrolysis of substrates of high molecular mass with multiple cleavage sites. mass-spectrometric studies (eriad) of the hydrolysis of the insulin b chain with trypsin, chymotrypsin, and proteinase i showed that monitoring of the ...	1988	3056418
[cryopreservation of early human embryo stages].	a short review of freezing procedures applied to early human embryos is given. it is noted that human embryos survived freezing and thawing at a developmental stage of 1. cell to blastocyst. but it seems to be necessary to use for any developmental stage of early embryo a special freezing and thawing method. embryo survival is correlated with their morphologic features where as neither age of embryos nor developmental stage were involved in freezing and thawing ability.	1988	3071049
regional localization of cd18, the beta-subunit of the cell surface adhesion molecule lfa-1, on human chromosome 21 by in situ hybridization.	the gene coding for the beta-subunit of the cell surface adhesion glycoprotein lfa-1 has been localized to the tip of the long arm of chromosome 21 at 21q22.1-qter.	1988	3073708
localization of the kras2 oncogene in the domestic rabbit (oryctolagus cuniculus).	the kras2 gene was localized in the rabbit by chromosomal in situ hybridization, using a 3h-labeled human cdna probe. there were 201 silver grains on 144 metaphase spreads; 12.9% of the grains resided on chromosome 16, and 54% of these grains were located close to the centromere at 16p11----q11. statistical analysis indicated that labeling at this region represents a significant deviation from a random distribution and thus provided evidence for the assignment of kras2 to 16p11----q11. in additi ...	1988	3073912
anaerobic infections in the head and neck region.	anaerobic bacteria form the predominant flora of the oral cavity, outnumbering facultative organisms by 10-1,000: 1. the type of anaerobic bacteria and their concentration depend on the anatomical site and the degree of anaerobiosis in the different sites in the mouth. three groups of anaerobic bacteria inhabit the oral cavity; the strict anaerobes, the moderate anaerobes, and the microaerophilic group of organisms. the majority of anaerobic bacterial infections occurring in the region of the mo ...	1988	3074469
specificity and retinotectal projections of quarter-eye fragments in xenopus laevis.	three quarters of the eye anlage in xenopus embryos of stage 33/34 were eliminated in three different sets of experiments. the remaining quadrant originated from the nasoventral part of the retina, from its ventral portion, or from the temporo-ventral area of the retina. all the fragments developed into small eyes of normal shape. the retinotectal connections did not deviate from those found in the control groups, even though mirror-image duplication was fairly frequent. for all fragments the te ...	1988	3077005
oestrus phases of the syrian hamster.		1988	2976222
immunobiological activities of a porin fraction isolated from fusobacterium nucleatum atcc 10953.	from fusobacterium nucleatum atcc 10953 cell envelope fraction whose inner membranes had been removed by treatment with sodium n-lauroyl sarcosinate, an outer membrane protein (37,000 mr in a native state) was prepared by extraction with lithium dodecyl sulfate. the protein thus obtained showed distinct porin activity, namely, the ability to form hydrophilic diffusion pores by incorporation into the artificial liposome membrane. the porin fraction exhibited strong immunobiological activities in ...	1988	2831155
evidence for a na+/h+ antiport in stomach smooth muscle cells.	single smooth muscle cells were isolated from circular muscle of the canine gastric corpus by collagenase incubation. cytoplasmic ph (phi) of these cells was measured fluorometrically using the trapped dye 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein. cells were examined for their na+/h+ exchange activity after intracellular acidification. cells acid-loaded by propionate exposure, the nh4+ prepulse technique or suspension in a na+-depleted medium regained almost normal phi upon exposure to a n ...	1988	2831889
high-performance liquid chromatographic separation and immunological characterization of soluble bovine viral diarrhea virus antigen.	sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blot analysis of the protein extracts from bovine viral diarrhea virus (bvdv, singer strain) infected primary calf testicle cells (soluble antigen) showed the presence of four virus specific polypeptides of 105, 90, 84 and 67 kilodaltons (kd) the 84-kd being the most abundant. anion-exchange high-performance liquid chromatography (hplc) of soluble antigen separated the virus specific polypeptides in individual peaks while the ...	1988	2832429
insulin-like growth factor i binding in hepatocytes from human liver, human hepatoma, and normal, regenerating, and fetal rat liver.	insulin-like growth factor-i (igf-i) in human hepatoma cells (hep-g2) has, in addition to its effect on cell growth, short-term metabolic effects acting through its own receptor. we have demonstrated that normal human hepatocytes, compared with hep-g2 cells, have virtually no igf-i binding sites. because the rate of growth is the major difference between the hepatoma and the normal liver, we asked if normal liver might express igf-i binding sites under physiologic growth conditions. indeed, wher ...	1988	2832449
a substituted thieno[3.4-d]imidazole versus substituted benzimidazoles as h+, k+-atpase inhibitors.	s 3337, 2-(2-ethylaminobenzylsulfinyl)-5,6-dimethoxybenzimidazole, and s 1924, 2-(5-methyl-2-picolylsulfinyl)-1h-thieno[3.4-d]imidazole, are members of new classes of h+, k+-atpase inhibitors. their effects on h+, k+-atpase and 14c-aminopyrine uptake in gastric glands were studied as well as in vivo in pylorus-ligated rats, stomach-lumen-perfused rats and heidenhain pouch dogs. their inhibitory effects were compared with the effect of omeprazole. in pylorus-ligated rats the two compounds showed ...	1988	2841691
ecology of colorado tick fever.		1988	2849373
[role of alpha-1 and alpha-2-adrenoreceptors in achieving the compensatory response of the hemostasis system to plasmin].		1988	2849566
antiviral chemoprophylaxis for cytomegalovirus infection.		1988	2849602
preparation by direct metal exchange and kinetic study of active site metal substituted class i and class ii clostridium histolyticum collagenases.	active site metal substitutions for both gamma- and zeta-collagenases from clostridium histolyticum have been made by direct metal exchange. the incubation of co(ii), cu(ii), ni(ii), cd(ii), and hg(ii) with these native collagenases results in changes in activity that parallel those observed for the reconstitution of the respective apoenzymes with these metal ions. for both collagenases, the exchange reactions with co(ii) and cu(ii) are complete within 1 min. however, the changes in activity obs ...	1988	2849992
mechanism of proline-specific proteinases: (i) substrate specificity of dipeptidyl peptidase iv from pig kidney and proline-specific endopeptidase from flavobacterium meningosepticum.	the substrate specificity of dipeptidyl peptidase iv (dipeptidyl peptide hydrolase, ec 3.4.14.5) from pig kidney and proline-specific endopeptidase from flavobacterium meningosepticum, was investigated with a series of n-terminal unprotected (dipeptidyl peptidases iv) and succinylated dipeptidyl-p-nitroanilides (proline-specific endopeptidase). both enzymes are specific for the s configuration of the amino-acid residue in p1 and p2 position if the penultimate residue is proline. in the case of a ...	1988	2896517
involvement of prostaglandins in histamine-induced fluid and electrolyte secretion by rat colon.	histamine increased the transmural potential difference across rat colon in-vivo and induced a net secretion of fluid. both effects were inhibited by indomethacin. histamine increased the potential difference and short-circuit current, and reduced tissue resistance in colonic sheets in-vitro. this response was reduced in the absence of chloride in the bathing medium or in the presence of serosal frusemide, suggesting that histamine stimulated electrogenic chloride secretion by the colon. the ris ...	1988	2897440
the effect of metoclopramide treatment on diabetic cystoparesis.		1988	2897479
comparative efficacy of ceftriaxone and rifampicin in eradicating pharyngeal carriage of group a neisseria meningitidis.	during an outbreak of meningococcal meningitis in saudi arabia, oral rifampicin (four doses in two days) was compared with a single intramuscular dose of ceftriaxone for prophylaxis in family contacts of patients with meningococcal disease. pharyngeal samples were taken for culture before and 1 and 2 weeks after administration. both follow-up cultures indicated that ceftriaxone was significantly more effective. at 1 week the eradication rates for ceftriaxone and rifampicin were 97% and 75%; at 2 ...	1988	2897515
neurotransmitters, cytokines, and the control of alveolar bone remodeling in orthodontics.	this article described research aimed at testing the hypothesis that tissue remodeling during orthodontic tooth movement is modulated, at least in part, by factors derived from the nervous and vascular (immune) systems. specifically, the neurotransmitters sp and vip and the cytokines il-1 alpha and il-1 beta were localized immunohistochemically in paradental tissues of cat canines that had been treated by the application of an 80 g tipping force for 1 hour to 14 days. increased staining (concent ...	1988	2900159
importance of pulmonary blood volume and aortic blood pressure in regulation of left ventricular function.		1988	2900297
localization of the mdx mutation within the mouse dystrophin gene.	we have mapped human and mouse x chromosome-specific genomic and cdna probes through an interspecies mus musculus/spretus pedigree which contains the mdx mutation. the positions of these markers relative to one another and to the mdx mutation were delineated. using probes corresponding to segments of the human duchenne muscular dystrophy (dmd) gene transcript, the position of a cross-hybridizing mouse equivalent gene (mdmd) was located. in more than 200 animals mapped, three were identified whic ...	1988	2903046
identification of a galactose-binding lectin on fusobacterium nucleatum fn-2.	a previous study has suggested that fusobacterium nucleatum fn-2 contains a galactose-binding protein (lectin) on the cell surface (p. a. murray, v. matarese, c. i. hoover, and j. r. winkler, fems microbiol. lett. 40:123-127, 1987). in the present study, the molecular specificity and size of this lectin were investigated by several techniques. whole-cell affinity chromatography with asialofetuin covalently coupled to sepharose 6mb demonstrated that 81% of 3h-labeled f. nucleatum were specificall ...	1988	2451642
[immunochemical study of the lipopolysaccharides of yersinia enterocolitica serovars o5 and o5.27].	y. enterocolitica lipopolysaccharides (lps), serovars o5 and o5.27, have similar antigenic specificity. the lps of serovar o5.27 has been shown to contain no factor o27. residual alpha-l-rhamnose, glycosylated by d-xylulose, has been found to play an important role in the formation of factor o5, common for both serovars.	1988	2469272
a new expression system for mammalian cells based on putative replicator sequences of the mouse and a truncated thymidine kinase gene.	we have constructed a new expression vector for mammalian cells. the vector contains a truncated tk gene for amplification under selective conditions, a sequence putatively supporting the replication of plasmid dna in eukaryotic cells (murine autonomously replicating sequence) and an expression cassette for the cdna to be studied. as a model cdna we have used that of human tissue-type plasminogen activator (t-pa). analysis of hirt supernatants and chromosomal dna from l cells, prepared six weeks ...	1988	3149608
[information processing for nursing. 4. nursing information and nursing records].		1988	259818
[operating assistant or operating room assistant (tvz, 17 july 1979)].		1988	259381
[the district nurse in the diabetes home care service in the province of limburg].		1988	259376
australian nursing.		1989	259844
divorce and its effects on children.	in any given case, the developmental stage of the child, the parental attitude, the financial situation, the custody battle, the visitation policies, the interactions between parents prior to and after divorce and the openness with which the subject is approached will interact to produce some effect, positive or negative, on the child. the question is, as professionals, what can we do to reduce the negative effects for this population at risk? it is comforting to note that the suffering caused b ...	1989	260790
breastfeeding protocol.		1989	260789
acute guillain-barré syndrome.		1989	2249465
hemorrhage due to retrograde prolapse of stomach. an endoscopic diagnosis.	a case of recurrent hemorrhage due to localized gastric mucosal congestion from retrograde prolapse during severe retching is described. historically, this condition may mimic the "mallory-weiss syndrome" and endoscopically, this lesion may be mistaken for "gastritis". this rarely recognized condition may be an explanation for some cases of cryptic hematemesis.	1989	313151
characterization of coaggregation between bacteroides gingivalis t22 and fusobacterium nucleatum t18.	bacterial adherence is a key factor in the colonization of the oral ecosystem, yet little is known about the mechanisms by which the pathogen bacteroides gingivalis adheres in the periodontal environment. we examined the ability of strains of b. gingivalis to coaggregate with selected microorganisms isolated from the subgingival microbiota of the cynomolgus monkey. a strong interaction was demonstrated between strains of b. gingivalis and fusobacterium nucleatum, whereas less pronounced or no in ...	1989	2478473
ovine and bovine abortion associated with fusobacterium nucleatum.		1989	2488355
[chemiluminescence response and phagocytic activity of murine polymorphonuclear leukocytes to various species of oral bacteria].	polymorphonuclear leukocytes (pmn) are predominant cells in the gingival crevice and saliva, and may play an important role in oral bacteria. murine peritoneal pmn was used and stimulated with 9 genera, 17 species of oral bacteria, including cariogenic and periodontal pathogens. the pmn response to the bacteria was measured by the luminol mediated chemiluminescence (cl) response and phagocytic activities, and the activities of lysozyme in the reaction medium after the cl response were also measu ...	1989	2489336
hemocyte monophenol oxidase activity in mosquitoes exposed to microfilariae of dirofilaria immitis.	monophenol oxidase (mpo) activity in hemocytes collected from aedes aegypti liverpool strain and aedes trivittatus intrathoracically inoculated with saline alone, inoculated with dirofilaria immitis microfilariae (mff), or from uninoculated mosquitoes was compared using a radiometric tyrosine hydroxylation assay. hemocyte mpo activity in mff-inoculated (= immune-activated) mosquitoes was significantly increased at 24 hr postinoculation (pi) in a. aegypti and at 6, 12, and 24 hr pi in a. trivitta ...	1989	2493084
[central pontine myelinolysis, associated with hyperglycemia and hyponatremia: a clinical case].	a 68 year old diabetic male was admitted with hyponatremia and developed a "deafferentiation" syndrome. central pontine myelinolysis was suspected after clinical, eeg and brain cat scan. the diagnosis was confirmed at necropsy. the pathogenesis, clinical manifestations and treatment of this disorder are discussed.	1989	2519379
bactericidal concentrations of chlorhexidine-digluconate, amine fluoride gel and stannous fluoride gel for subgingival bacteria tested in serum at short contact times.	in vitro inhibitory and bactericidal concentrations in serum of chlorhexidine-digluconate, amine fluoride gel, stannous fluoride gel, stannous fluoride, metronidazole and amoxicillin were determined against bacteroides gingivalis, bacteroides intermedius, fusobacterium nucleatum, actinobacillus actinomycetemcomitans and capnocytophaga sputigena. the minimal inhibitory concentration was assessed by the agar dilution technique. the killing curves and minimal bactericidal concentration of the antim ...	1989	2524581
[gene cloning of an actinobacillus actinomycetemcomitans y4 antigen that reacts to peripheral blood sera in advanced destructive periodontitis patients].	actinobacillus actinomycetemcomitans (a.a.) has been implicated in the etiology of juvenile periodontitis and also of advanced destructive periodontitis (adp). it has been reported that the levels of the igg antibody against the a.a. in the peripheral blood sera of the adp patients were often high as well as those against the bacteroides gingivalis. to clone the genes of antigen reactive to the sera of the adp patients, we constructed a phage library of the a.a. strain y4 dna in lambda l47, whic ...	1989	2533606
lectinlike interactions of fusobacterium nucleatum with human neutrophils.	fusobacterium nucleatum expresses lectinlike adherence factors which mediate binding to a variety of human tissue cells. adherence is selectively inhibited by galactose, lactose, and n-acetyl-d-galactosamine. in this study, adherence of f. nucleatum to human peripheral blood polymorphonuclear neutrophils (pmns) was investigated. the results indicated that the fusobacteria adhered to live and metabolically inactivated or fixed pmns. adherence of f. nucleatum resulted in activation of pmns as dete ...	1989	2553609
unlikely association between clinically apparent herpesvirus infection and coronary incidence at older ages. the framingham heart study.	experimental studies in chickens have shown a relationship of a herpesvirus to atherosclerosis. the hypothesis of an association in humans was tested by using data on the history of cold sores and other manifestations of herpes infections reported by 658 male and 919 female participants (ages 58 to 89) in the framingham heart study from 1977 to 1979 and on the prevalence and subsequent 6-year incidence of coronary heart disease (chd). approximately 40% of the men and 52% of the women reported a ...	1989	2556099
absorption of human growth hormone from the rat lung.	recombinant methionyl human growth hormone (hgh) was administered intratracheally to adult rats, and serum concentrations of immunoreactive hgh were measured for up to 24 h. the mean absolute bioavailability was approximately 36% after 18h and was similar for doses of 0.75, 1.5, and 3 mg/kg. peak serum hgh concentrations occurred at approximately 6 h after dosing. tritiated hgh (3h-hgh) was used to follow the clearance of hormone from the lungs. disappearance was linear with time and by 24 h app ...	1989	2562650