Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| ecology and epidemiology of spotted fever group rickettsiae and new data from their study in russia and kazakhstan. | rickettsiae represent a wide range of pathogenicity from classic and new pathogens to endosymbionts of eukaryotic cells. recent studies of rickettsiae have widened the number of representatives of genus rickettsia, especially in the spotted fever group (sfg). rickettsiae of sfg are tick-borne microorganisms with effective transovarial and transstadial transmission. the main hosts are ticks (dermacentor, rhipicephalus, haemophysalis, ixodes, and amlyomma). strategy of maintenance of tick microorg ... | 2003 | 12860595 |
| rickettsialpox in new york city: a persistent urban zoonosis. | rickettsialpox, a spotted fever rickettsiosis, was first identified in new york city (nyc) in 1946. during the next five years, approximately 540 additional cases were identified in nyc. however, during the subsequent five decades, rickettsialpox received relatively little attention from clinicians and public health professionals, and reporting of the disease diminished markedly. during february 2001 through august 2002, 34 cases of rickettsialpox in nyc were confirmed at cdc from cutaneous biop ... | 2003 | 12860597 |
| physician knowledge of the diagnosis and management of rocky mountain spotted fever: mississippi, 2002. | rocky mountain spotted fever (rmsf), a tick-borne illness that has its highest incidence in the south central and southeastern united states, is often a diagnostic challenge, as patients frequently present with nonspecific symptoms during the early stages of illness. rmsf has a high case fatality rate among untreated individuals, and the median time from onset of symptoms to death is only eight days, making early recognition and treatment of rmsf crucial. in two mississippi public health distric ... | 2003 | 12860642 |
| rickettsia rickettsii infection in the pine vole, microtus pinetorum: kinetics of infection and quantitation of antioxidant enzyme gene expression by rt-pcr. | the pine vole, microtus pinetorum, was evaluated as a laboratory animal model for infection with rickettsia rickettsii. voles demonstrated signs of acute disease, and 45% of infected animals died following intraperitoneal infection with 3 x 10(6) plaque forming units of r. rickettsii. spleen, liver, kidney, lung, brain, testes and blood were analyzed for rickettsial burden by a quantitative pcr assay. the distribution of rickettsiae in tissues during the course of infection was determined by imm ... | 2003 | 12860675 |
| rickettsial actin-based motility: behavior and involvement of cytoskeletal regulators. | actin-based motility (abm) is employed by spotted fever group (sfg) rickettsiae, such as rickettsia rickettsii, to promote cell-to-cell spread. time-lapse video microscopy revealed that abm is not strictly confined to sfg rickettsiae as typhus group r. typhi moved at approximately the same rate as r. rickettsii (approximately 4 micro m/min), but in a highly erratic fashion. a number of common behaviors were observed between abm of r. typhi and r. rickettsii, such as entrance into plasma membrane ... | 2003 | 12860687 |
| interactions of rickettsia rickettsii with endothelial nuclear factor-kappab in a "cell-free" system. | 2003 | 12860701 | |
| genetic analysis of isolates of rickettsia rickettsii that differ in virulence. | we examined the degree of intraspecies genetic polymorphisms present among 13 r. rickettsii strains isolated from the blood of patients and ixodid ticks from north and south america. preliminary confirmation of these isolates as r. rickettsii was done by evaluating rsai and psti restriction fragment length polymorphic (rflp) sites in a 631 bp fragment of the rompa gene amplified with primers encompassing the rr190.70-701 nt region. all isolates had the same profile, which was identical to that p ... | 2003 | 12860712 |
| molecular and functional analysis of the lepb gene, encoding a type i signal peptidase from rickettsia rickettsii and rickettsia typhi. | the type i signal peptidase lepb genes from rickettsia rickettsii and rickettsia typhi, the etiologic agents of rocky mountain spotted fever and murine typhus, respectively, were cloned and characterized. sequence analysis of the cloned lepb genes from r. rickettsii and r. typhi shows open reading frames of 801 and 795 nucleotides, respectively. alignment analysis of the deduced amino acid sequences reveals the presence of highly conserved motifs that are important for the catalytic activity of ... | 2003 | 12867468 |
| serologic evidence of infection with ehrlichiae and spotted fever group rickettsiae among residents of gag island, indonesia. | the causative agents of scrub and murine typhus are considered endemic to indonesia. however, the presence of spotted fever group rickettsiae and ehrlichiae have not been previously described in this country. during an investigation of arthropod-borne diseases on gag island, located northwest of the island of new guinea in eastern indonesia, the prevalence of antibody to the etiologic agents of monocytic ehrlichiosis, spotted fever rickettsiosis, and scrub and murine typhus were determined. anal ... | 2003 | 12875301 |
| the immunologic response of dogs to bartonella vinsonii subspecies berkhoffii antigens: as assessed by western immunoblot analysis. | bartonella vinsonii subspecies berkhoffii is a recently recognized zoonotic pathogen that causes endocarditis, granulomatous rhinitis, and granulomatous lymphadenitis in dogs. isolation of b. vinsonii (berkhoffii) from blood or tissue samples is frequently unsuccessful; therefore, diagnosis is primarily dependent on serologic or molecular testing modalities. because previous canine serologic studies have used an indirect immunofluorescence assay (ifa), without western immunoblot (wi) confirmatio ... | 2003 | 12918816 |
| cross-protection between distantly related spotted fever group rickettsiae. | two excellent c3h/hen mouse models of spotted fever rickettsioses caused by the distantly related organisms, rickettsia conorii and rickettsia australis, were utilized to evaluate the possibility of the stimulation of broad cross-protective immunity. sublethal infection stimulated complete immunity, that is absence of disease, after challenge with a dose of the heterologous rickettsia that uniformly killed naïve mice. in contrast, heterologous immune sera did not protect mice against a lethal do ... | 2003 | 12922124 |
| spotted fever rickettsiosis in coronel fabriciano, minas gerais state. | we report cases of spotted fever rickettsiosis in coronel fabriciano municipality of minas gerais state, brazil. the cases occurred in may and june of 2000. during this period there were two deaths among children from an area named pedreira in a periurban area of this municipality. in a boy who died with clinical manifestations of brazilian spotted fever, a necropsy revealed the presence of a spotted fever group rickettsia. the serological results confirm the difficulty in the differential diagn ... | 2003 | 12937725 |
| serologically detected "new" tick-borne zoonoses in eastern croatia. | to establish serologically a contact with causative agents of human monocytic and granulocytic ehrlichiosis, human babesiosis, recently detected rickettsioses, and lyme disease in individuals with a history of tick bite from three counties in eastern croatia. | 2003 | 14515426 |
| [study on spotted fever group rickettsiae in guangdong province]. | to explore the existence of spotted fever group rickettsiae (sfgr) in guangdong province. | 2003 | 14521792 |
| mycobacterium marinum escapes from phagosomes and is propelled by actin-based motility. | mycobacteria are responsible for a number of human and animal diseases and are classical intracellular pathogens, living inside macrophages rather than as free-living organisms during infection. numerous intracellular pathogens, including listeria monocytogenes, shigella flexneri, and rickettsia rickettsii, exploit the host cytoskeleton by using actin-based motility for cell to cell spread during infection. here we show that mycobacterium marinum, a natural pathogen of fish and frogs and an occa ... | 2003 | 14597736 |
| isolation of bartonella washoensis from a dog with mitral valve endocarditis. | we report the first documented case of bartonella washoensis bacteremia in a dog with mitral valve endocarditis. b. washoensis was isolated in 1995 from a human patient with cardiac disease. the main reservoir species appears to be ground squirrels (spermophilus beecheyi) in the western united states. based on echocardiographic findings, a diagnosis of infective vegetative valvular mitral endocarditis was made in a spayed 12-year-old female doberman pinscher. a year prior to presentation, the re ... | 2003 | 14605197 |
| implications of presumptive fatal rocky mountain spotted fever in two dogs and their owner. | a dog was examined because of petechiation, an inability to stand, pale mucous membranes, a possible seizure, and thrombocytopenia. tick-borne illness was suspected, but despite treatment, the dog died. eight days later, a second dog owned by the same individual also died. the dog was not examined by a veterinarian, but rocky mountain spotted fever (rmsf) was suspected on the basis of clinical signs. two weeks after the second dog died, the owner was examined because of severe headache, fever, n ... | 2003 | 14627095 |
| evaluation of a pcr assay for quantitation of rickettsia rickettsii and closely related spotted fever group rickettsiae. | a spotted fever rickettsia quantitative pcr assay (sq-pcr) was developed for the detection and enumeration of rickettsia rickettsii and other closely related spotted fever group rickettsiae. the assay is based on fluorescence detection of sybr green dye intercalation in a 154-bp fragment of the rompa gene during amplification by pcr. as few as 5 copies of the rompa gene of r. rickettsii can be detected. sq-pcr is suitable for quantitation of r. rickettsii and 10 other genotypes of spotted fever ... | 2003 | 14662926 |
| rocky mountain spotted fever as a cause of macular star figure. | an 86-year-old woman with a history of tick bites in the previous months developed subnormal visual acuity in both eyes, keratic precipitates, anterior chamber and vitreous cells, optic disc edema, retinal hemorrhages, and retinal arteriolar sheathing. she had no fever or skin rash. three weeks later, binocular macular star figures appeared. brain imaging was negative; cerebrospinal fluid disclosed a lymphocytic pleocytosis and elevated protein. the serum rickettsia rickettsii antibody test was ... | 2003 | 14663310 |
| spotted-fever-group rickettsioses in north asia. | 2003 | 14667766 | |
| fatal spotted fever rickettsiosis, minas gerais, brazil. | the emergence and reemergence of a serious infectious disease are often associated with a high case-fatality rate because of misdiagnosis and inappropriate or delayed treatment. the current reemergence of spotted fever rickettsiosis caused by rickettsia rickettsii in brazil has resulted in a high proportion of fatal cases. we describe two familial clusters of brazilian spotted fever in the state of minas gerais, involving six children 9 months to 15 years of age; five died. immunohistochemical i ... | 2003 | 14718082 |
| antinuclear antibodies can be detected in dog sera reactive to bartonella vinsonii subsp. berkhoffii, ehrlichia canis, or leishmania infantum antigens. | the presence of antinuclear antibodies (anas) is used to support a clinical diagnosis of systemic lupus erythematosus (sle) in dogs. however, clinicians must interpret the detection of anas with caution, particularly in light of increasing evidence that dogs with known bacterial and protozoal infections can have high ana titers. retrospectively, medical records were reviewed for all dogs that were concurrently tested for antinuclear antigens and bartonella vinsonii (berkhoffii), ehrlichia canis, ... | 2004 | 14765731 |
| rickettsia parkeri: a newly recognized cause of spotted fever rickettsiosis in the united states. | ticks, including many that bite humans, are hosts to several obligate intracellular bacteria in the spotted fever group (sfg) of the genus rickettsia. only rickettsia rickettsii, the agent of rocky mountain spotted fever, has been definitively associated with disease in humans in the united states. herein we describe disease in a human caused by rickettsia parkeri, an sfg rickettsia first identified >60 years ago in gulf coast ticks (amblyomma maculatum) collected from the southern united states ... | 2004 | 14999622 |
| nf-kappab activation suppresses host cell apoptosis during rickettsia rickettsii infection via regulatory effects on intracellular localization or levels of apoptogenic and anti-apoptotic proteins. | rickettsia rickettsii, a gram-negative and obligate intracellular bacterium, is the causative agent of rocky mountain spotted fever. in human infections, the primary target of r. rickettsii infection is vascular endothelium. our laboratory has shown that activation of nuclear transcription factor-kappa b (nf-kappab) during r. rickettsii infection of cultured human endothelial cells protects against apoptosis by preventing the activation of apical caspases-8 and -9, and the effector caspase-3. to ... | 2004 | 15135541 |
| fatal cases of rocky mountain spotted fever in family clusters--three states, 2003. | rocky mountain spotted fever (rmsf), a tickborne infection caused by rickettsia rickettsii and characterized by a rash, has a case-fatality rate as high as 30% in certain untreated patients. even with treatment, hospitalization rates of 72% and case-fatality rates of 4% have been reported. this report summarizes the clinical course of three fatal cases of rmsf in children and related illness in family members during the summer of 2003. these cases underscore the importance of 1) prompt diagnosis ... | 2004 | 15152183 |
| rocky mountain spotted fever at koair children's hospital, 1990-2002. | the reported average annual incidence of rocky mountain spotted fever (rmsf) in kentucky is less than 5 per million population, although seroprevalence studies suggest that exposure to rickettsia riskettsii, the causative agent, is relatively common among children. the experience with rmsf at kosair children's hospital over a 12-year period was reviewed. fifteen cases were identified (5 boys and 10 girls). illness onset ranged from april to october, and 4 patients resided in jefferson county. th ... | 2004 | 15152446 |
| a rickettsia wasp-like protein activates the arp2/3 complex and mediates actin-based motility. | spotted fever group rickettsia are obligate intracellular pathogens that exploit the host cell actin cytoskeleton to promote motility and cell-to-cell spread. although other pathogens such as listeria monocytogenes use an arp2/3 complex-dependent nucleation mechanism to generate comet tails consisting of y-branched filament arrays, rickettsia polymerize tails consisting of unbranched filaments by a previously unknown mechanism. we identified genes in several rickettsia species encoding proteins ... | 2004 | 15236643 |
| prevalence of antibodies to spotted fever group rickettsiae in humans and domestic animals in a brazilian spotted fever-endemic area in the state of são paulo, brazil: serologic evidence for infection by rickettsia rickettsii and another spotted fever group rickettsia. | in serum samples obtained from all the healthy humans, horses, dogs, and donkeys present on three farms in the pedreira municipality, an endemic area for brazilian spotted fever, an indirect immunofluorescence assay (ifa) detected antibodies against rickettsia rickettsii in 17 (77.3%) horses, 5 (31.3%) dogs (titers ranging from 64 to 4,048), and none of 4 donkeys or 50 humans. five canine and eight equine sera with high antibody titers to r. rickettsii were also tested by ifa against r. bellii, ... | 2004 | 15238696 |
| bartonella henselae igg antibodies are prevalent in dogs from southeastern usa. | in contrast to the large body of literature regarding bartonella henselae in humans and cats, there is little information about b. henselae as an infectious agent in dogs. due to the paucity of information regarding the b. henselae serology in dogs, we performed a cross-sectional serosurvey using b. henselae antigen in order to compare the seroprevalence between sick and healthy dogs from the south-eastern usa. ninety-nine sera were collected from clinically healthy dogs. three hundred and one s ... | 2004 | 15369661 |
| rickettsial vaccines: the old and the new. | in the past century vaccine development for prevention of rickettsial diseases has been prolific. however, in the past 20 years no new rickettsial vaccine has been manufactured and there are currently no new or old rickettsial vaccines licensed. early rickettsial vaccines were difficult, expensive and very hazardous to produce. molecular biology techniques of today are currently being used to develop new rickettsial vaccines that are standardized, inexpensive, nonhazardous and efficacious. | 2004 | 15485334 |
| transfusion-transmitted tick-borne infections: a cornucopia of threats. | over the past several decades, the frequency of contact between humans and ticks has increased dramatically. concomitantly, several newly recognized tick-borne pathogens have emerged joining those already known to be transmitted by ticks. together these factors have led to an enhanced public health awareness of ticks, tick-borne agents, and their associated diseases. reports that several of these agents are transmitted by blood transfusion have raised concerns about blood safety. the primary age ... | 2004 | 15497129 |
| sequence and expression analysis of the ompa gene of rickettsia peacockii, an endosymbiont of the rocky mountain wood tick, dermacentor andersoni. | the transmission dynamics of rocky mountain spotted fever in montana appears to be regulated by rickettsia peacockii, a tick symbiotic rickettsia that interferes with transmission of virulent rickettsia rickettsii. to elucidate the molecular relationships between the two rickettsiae and glean information on how to possibly exploit this interference phenomenon, we studied a major rickettsial outer membrane protein gene, ompa, presumed to be involved in infection and pathogenesis of spotted fever ... | 2004 | 15528527 |
| rickettsia species infecting amblyomma cooperi ticks from an area in the state of são paulo, brazil, where brazilian spotted fever is endemic. | owing to the potential role of the tick amblyomma cooperi in the enzootic cycle of rickettsia rickettsii, the etiologic agent of brazilian spotted fever (bsf), this study evaluated infection by rickettsia species in a. cooperi ticks collected from an area in brazil where bsf is endemic. among a total of 40 a. cooperi adult ticks collected in an area of bsf endemicity in the state of são paulo, pcr analysis detected dna of rickettsia bellii in 16 ticks (40%), and 3 other ticks (7.5%) were positiv ... | 2004 | 14715737 |
| colonic adrenocarcinomas in patients with ureterosigmoidostomies. | two additional cases of adenocarcinoma of the sigmoid colon occurring at the site of ureterosigmoidostomy are reported. the high risk of this complication occurring in patients who have undergone ureterosigmoidostomy is described. the possible shortness of the interval from diversion to development of an adenocarcinoma, especially in those cases involving carcinoma of the bladder, is emphasized. it is strongly recommended that careful follow up of such patients should begin within several years ... | 2004 | 446246 |
| [the late david kohn, m.d]. | 2004 | 321306 | |
| theory and development of transcutaneous oxygen pressure measurement. | 2004 | 3323065 | |
| [our experience in using artificial bands for ligamentoplasty in the knee region (author's transl)]. | 2004 | 509254 | |
| five-year findings of the hypertension detection and follow-up program. ii. mortality by race-sex and age. hypertension detection and follow-up program cooperative group. | data are reported for four race-sex and three age subgroups of the hypertension detection and follow-up program (hdfp). throughout the hdfp trial, for black men, black women, white men, and white women and for persons aged 30 to 49, 50 to 59, and 60 to 69 years at entry, control of blood pressure was consistently better for stepped care (sc) than referred care (rc) participants. this difference in degree of control was least for white women; it was less for whites than for blacks of the same sex ... | 2004 | 490883 |
| coronary artery aneurysm. | the case is presented of a 28-year-old woman with systemic vasculitis, aortic insufficiency, and an aneurysm of the proximal right coronary artery. more than 100 patients with coronary artery aneurysm have been reported, and this represents the twenty-first case treated surgically. coronary artery aneurysms occur more frequently than previously realized and are seen in approximately 1.5% of patients with coronary artery disease. in addition to arteriosclerotic aneurysm, other common types of cor ... | 2004 | 305770 |
| [theodor billroth (1829-1894), "pope of surgery", on the 150th anniversary of his birth]. | 2004 | 375156 | |
| [contribution of the section medical pedagogy to the improvement of quality and effectiveness of health care]. | 2004 | 425577 | |
| provision of a lingual biting platform in a severe class iii malocclusion: a case report. | 2004 | 321730 | |
| the treatment of acute cholecystitis. a series of 497 consecutive patients. | in a five-year series of 497 consecutive patients with acute cholecystitis an overall mortality of 3.2% was observed. there was no significant difference in mortality between patients undergoing early surgery and those managed initially conservatively. the total incidence of postoperative complications was also similar in the early and delayed surgery groups, although wound complications were slightly more frequent after early surgery. a special risk group was formed by patients with unsuccessfu ... | 2004 | 525185 |
| a sporadic case of legionnaires' disease in belgium. | 2004 | 525201 | |
| [a case report of portal-systemic encephalopathy with normal portal vein pressure and non-cirrhosis of the liver]. | 2004 | 3320417 | |
| [the control of renin secretion: experimental data and clinical implications]. | 2004 | 356832 | |
| [clinico-immunologic shifts in myocardial infarct]. | 2005 | 1274315 | |
| [the problems of cardiovascular pathology in adolescents]. | 2005 | 3320454 | |
| [treatment of parkinsonism]. | 2005 | 1265907 | |
| clinical evaluation of tumor scanning with 169-yb-citrate. | 169-yb-citrate was used as the tumor-scanning agent in 360 patients with malignant tumors and in 55 others with benign lesions and the results compared with 67-ga-citrate studies in other series. in malignant tumors, the overall positive detection rate for ytterbium was 65.3%; in benign lesions, the false-positive rate was 29.1%. good results were obtained in the extremities, head and neck, and pelvic area. of all tumors visualized, squamous cell carcinoma was detected most effectively (77.5%). ... | 2005 | 1153741 |
| [laryngeal suspension microsurgery]. | 2005 | 1230948 | |
| glutaric aciduria type i: unusual biochemical presentation. | we describe a patient with glutaryl-coenzyme a dehydrogenase deficiency, demonstrated by a residual enzyme activity of only 1% in cultured fibroblasts. although the clinical presentation was typical of glutaric aciduria type i, the urine concentrations of glutaric, glutaconic, and 3-hydroxyglutaric acids remained normal, even during episodes of clinical decompensation. an increased free glutarate level was demonstrated only in cerebrospinal fluid. | 2005 | 1625098 |
| experimental evidence of ischemic thresholds and functional recovery. | impaired blood flow below certain critical levels and an insufficient supply of energy-rich substrates cause failure of neuronal function, triggering biochemical disturbances that eventually lead to ischemic cell damage. | 2005 | 1440719 |
| the interaction between legalization of abortion and contraception in denmark. | trends in fertility, abortion, and contraceptive practice in denmark were analyzed, using previously compiled official statistics; the conclusion was drawn that easy access to abortion may contribute toward a decline in contraceptive practice depending on the level of contraceptive practice in the population and on the degree of confidence the population has in available contraceptive methods. in october 1973 denmark passed a law permitting women to obtain free abortion on demand. the number of ... | 2005 | 538953 |
| [morbidity with temporary loss of work capacity in the workers of the belmeken-sestrimo hydroelectric cascade]. | the present work presents the results from the analysis on the morbidity with temporary disability from cascade "belmeken-sestrimo" (n = 94, men 73, women 21). by means of personnel method are studied the cases of morbidity with temporary disability and defined the basic and enlarged number of indices, analysed in sex, age, length of service, nosological structure and dynamics for a period of 3 years (1987-1989) for the 3 sites of hydroelectric cascade "belmeken-sestrimo", water-power station "m ... | 2005 | 1364540 |
| a volumetric assessment of pollen levels in the air of ann arbor, michigan. | 2005 | 1196148 | |
| serologic survey of select infectious diseases in coyotes and raccoons in nebraska. | to obtain data about select zoonotic and other infectious diseases in free-ranging predators in five ecoregions in nebraska, sera were collected from 67 coyotes (canis latrans) and 63 raccoons (procyon lotor) from november 2002 through january 2003. for coyotes, antibodies were detected against canine distemper virus (cdv, 61%), francisella tularensis (32%), rickettsia rickettsi (13%), and flaviviruses (48%). none of the coyote sera had antibodies to borrelia burgdorferi, brucella canis, or six ... | 2005 | 16456169 |
| high-throughput molecular testing of ticks using a liquid-handling robot. | to meet the need for high-throughput sample testing, dna extraction kits based on the 96-well plate format have been developed for use with blood and tissue samples. these methods have not been applied to dna extractions from ticks. to meet this need, we developed a high-throughput method for dna extraction and polymerase chain reaction (pcr) testing of tick samples. a liquid-handling robot was used to extract dna in a 96-well binding column plate with vacuum manifold. the quantity, purity, and ... | 2005 | 16465749 |
| autosomal recessive inheritance of nager acrofacial dysostosis. | nager acrofacial dysostosis is a variant of mandibulofacial dysostosis with severe micrognathia, malar hypoplasia, and radial limb defects. most cases are sporadic, but autosomal recessive inheritance has been suggested. a family is reported in which two sibs are affected by this syndrome, presenting further evidence for autosomal recessive inheritance. the recognition of this syndrome as a distinct entity has important implications. after the birth of a child with orofacial malformations sugges ... | 2005 | 3367347 |
| [immunotherapeutic approach in tuberculosis: thymus hormones]. | 2005 | 1287758 | |
| toxoplasmosis after splenectomy in sickle cell disease. report of two cases with recurrence in one child two years following antitoxoplasmic therapy. | 2005 | 1253508 | |
| [the metastic development of breast carcinoma after surgical treatment of primary tumor. iv]. | 2005 | 1143694 | |
| [lymphocyte populations in hbag-positive acute hepatitis]. | 2005 | 1087387 | |
| milk carbohydrates of marsupials. ii. quantitative and qualitative changes in milk carbohydrates during lactation in the tammar wallaby (macropus eugenii). | milk was collected at various stages of lactation from a group of tammar wallabies, m. eugenii, in which parturition had been synchronized. the milk carbohydrate was determined by a phenol-sulfuric acid method which had been modified to give equal colour yields for galactose and glucose. the mean carbohydrate content increased gradually during the first 6 months of lactation to a peak of 13 g hexose/100 ml of milk, but then fell rapidly to much lower values, over the following 2 months. througho ... | 2005 | 549552 |
| nf-kappab activation during rickettsia rickettsii infection of endothelial cells involves the activation of catalytic ikappab kinases ikkalpha and ikkbeta and phosphorylation-proteolysis of the inhibitor protein ikappabalpha. | rocky mountain spotted fever, a systemic tick-borne illness caused by the obligate intracellular bacterium rickettsia rickettsii, is associated with widespread infection of the vascular endothelium. r. rickettsii infection induces a biphasic pattern of the nuclear factor-kappab (nf-kappab) activation in cultured human endothelial cells (ecs), characterized by an early transient phase at 3 h and a late sustained phase evident at 18 to 24 h. to elucidate the underlying mechanisms, we investigated ... | 2005 | 15618150 |
| functional analysis of seca homologues from rickettsiae. | the molecular basis of protein secretion that underlines rickettsial pathogenesis remains unknown. this paper reports the molecular and functional analysis of the putative seca gene, an essential component of the sec-dependent protein secretion pathway, from rickettsia rickettsii and rickettsia typhi, the aetiological agents of rocky mountain spotted fever and murine typhus, respectively. the sequence analysis of the cloned seca genes from r. rickettsii and r. typhi show orfs of 2721 and 2718 nt ... | 2005 | 15699207 |
| rickettsial infection in animals and brazilian spotted fever endemicity. | we compared the rickettsial infection status of amblyomma cajennense ticks, humans, dogs, and horses in both brazilian spotted fever (bsf)-endemic and -nonendemic areas in the state of sao paulo, brazil. most of the horses and few dogs from bsf-endemic areas had serologic titers against rickettsia rickettsii antigens. in contrast, no dogs or horses from bsf-nonendemic areas had serologic titers against r. rickettsii antigens, although they were continually exposed to a. cajennense ticks. all hum ... | 2005 | 15752445 |
| changes in the adherens junctions of human endothelial cells infected with spotted fever group rickettsiae. | rickettsiae of the spotted fever group are obligately intracellular bacteria that primarily infect the vascular endothelium, invade adjacent cells propelled by actin polymerization, and cause severe systemic diseases. endothelial dysfunction and vascular leakage develop as a consequence; this effect is the pathophysiological mechanism that explains most clinical manifestations. here we report that rickettsial infection of cultured primary human endothelial cells is associated with the formation ... | 2005 | 15778845 |
| [rocky mountain spotted fever in an american tourist]. | in a 28-year-old male american tourist who presented in the hospital with fever, cold shivers, headache, nausea, myalgia and arthralgia, rocky mountain spotted fever was suspected, partly because he came from an endemic region (the state of georgia). the patient was treated with doxycycline, 100 mg b.i.d.; 9 days after the first appearance of the symptoms, the diagnosis was confirmed by the report of a positive antibody titre against rickettsia rickettsii. the patient did not have exanthema. he ... | 2005 | 15835630 |
| central nervous system dysfunction associated with rocky mountain spotted fever infection in five dogs. | five dogs from the northeastern united states were presented with clinical signs of neurological disease associated with rocky mountain spotted fever (rmsf) infection. four of the five dogs had vestibular system dysfunction. other neurological signs included paresis, tremors, and changes in mentation. all of the dogs had an elevated indirect fluorescent antibody titer or a positive semiquantitative enzyme screening immunoassay titer for rickettsia rickettsii at the time of presentation. although ... | 2005 | 15995164 |
| rocky mountain spotted fever--changing ecology and persisting virulence. | 2005 | 16093463 | |
| rocky mountain spotted fever from an unexpected tick vector in arizona. | rocky mountain spotted fever is a life-threatening, tick-borne disease caused by rickettsia rickettsii. this disease is rarely reported in arizona, and the principal vectors, dermacentor species ticks, are uncommon in the state. from 2002 through 2004, a focus of rocky mountain spotted fever was investigated in rural eastern arizona. | 2005 | 16093467 |
| detection of brazilian spotted fever infection by polymerase chain reaction in a patient from the state of são paulo. | brazilian spotted fever (bsf) cases have been increasing in the state of são paulo but no genomic information about local rickettsia isolated from humans has been well documented. we recovered spotted-fever group rickettsiae from a sample of patient blood cultured in vero cells using the shell vial technique. rickettsial dna fragments (glta, ompa, and, ompb genes) were detected, and analysis of the ompb gene base sequences showed identity with the rickettsia rickettsii ompb sequence available in ... | 2005 | 16113868 |
| expression and secretion of chemotactic cytokines il-8 and mcp-1 by human endothelial cells after rickettsia rickettsii infection: regulation by nuclear transcription factor nf-kappab. | infection of endothelial cells (ec) with rickettsia rickettsii results in rocky mountain spotted fever, an acute illness characterized by systemic inflammation. interleukin-8 (il-8) and monocyte chemoattractant protein-1 (mcp-1) are important chemokines for activating neutrophils and monocytes, respectively, and recruiting these circulating immune cells to the sites of inflammation. in this study, we have measured the expression and secretion of these chemokines during r. rickettsii infection of ... | 2005 | 16128401 |
| activation of p38 stress-activated protein kinase during rickettsia rickettsii infection of human endothelial cells: role in the induction of chemokine response. | rickettsia rickettsii, a gram-negative and obligate intracellular bacterium, preferentially infects the vascular endothelium during human infections leading to inflammation and dysfunction. the aim of this study was to determine whether r. rickettsii infection of endothelial cells (ec) activates p38 and/or c-jun n-terminal kinases (jnk) mitogen-activated protein (map) kinase, key regulatory proteins that control the response to inflammatory stimuli. we show that infection of cultured human ec re ... | 2005 | 16153249 |
| cardiac involvement in a patient with clinical and serological evidence of african tick-bite fever. | myocarditis and pericarditis are rare complications of rickettsiosis, usually associated with rickettsia rickettsii and r. conorii. african tick-bite fever (atbf) is generally considered as a benign disease and no cases of myocardial involvement due to rickettsia africae, the agent of atbf, have yet been described. | 2005 | 16242016 |
| evaluation of a western blot method for the detection of yersinia antibodies: evidence of serological cross-reactivity between yersinia outer membrane proteins and borrelia burgdorferi. | yersinia enterocolitica and yersinia pseudotuberculosis have been identified as causative organisms of reactive arthritis in humans. we evaluated a western blot assay which uses yersinia outer membrane proteins as antigens for the detection of yersinia antibodies as a replacement for the complement fixation (cf) assay. clinical agreement, sensitivity, and specificity were determined by testing 19 positive and 21 negative serum samples by the cf assay, western blot assay, and enzyme-linked immuno ... | 2005 | 16275939 |
| serological survey of vector-borne zoonotic pathogens in pet cats and cats from animal shelters and feral colonies. | although cats and their arthropod parasites can sometimes be important sources of zoonotic diseases in humans, the extent of exposure among various cat populations to many potential zoonotic agents remains incompletely described. in this study, 170 domestic cats living in private homes, feral cat colonies, and animal shelters from california and wisconsin were evaluated by serology to determine the levels of exposure to a group of zoonotic vector-borne pathogens. serological positive test result ... | 2006 | 16434226 |
| detection of rickettsia rickettsii in the tick amblyomma cajennense in a new brazilian spotted fever-endemic area in the state of minas gerais. | the present study evaluated rickettsial infection in amblyomma spp. ticks collected in a farm in coronel pacheco, a brazilian spotted fever (bsf) endemic area. a total of 78 a. cajennense and 78 a. dubitatum free-living adult ticks were collected and tested by polymerase chain reaction (pcr) targeting a fragment of the rickettsial gene glta. only one pool of three a. cajennense ticks showed the expected product by pcr. this pool was further tested by pcr using sets of primers targeting the ricke ... | 2006 | 16444414 |
| antibodies to rickettsia rickettsii, rickettsia typhi, coxiella burnetii, bartonella henselae, bartonella quintana, and ehrlichia chaffeensis among healthy population in minas gerais, brazil. | rickettsial diseases except those belonging to spotted fever group rickettsioses are poorly studied in south america particularly in brazil where few epidemiological reports have been published. we describe a serosurvey for rickettsia rickettsii, r. typhi, coxiella burnetii, bartonella henselae, b. quintana, and ehrlichia chaffeensis in 437 healthy people from a brazilian rural community. the serum samples were tested by indirected micro-immunoflourescence technique and a cutoff titer of 1:64 wa ... | 2006 | 16444416 |
| experimentally infected human body lice (pediculus humanus humanus) as vectors of rickettsia rickettsii and rickettsia conorii in a rabbit model. | the human body louse, the natural vector of rickettsia prowazekii, is able to experimentally transmit the normally flea-borne rickettsia r. typhi, suggesting that the relationships between the body louse and rickettsiae are not specific. we used our experimental infection model to test the ability of body lice to transmit two prevalent tick-borne rickettsiae. each of two rabbits was made bacteremic by injecting intravenously 2 x 10(6) plaque-forming units of either r. rickettsii or r. conorii. f ... | 2006 | 16606977 |
| rocky mountain spotted fever. | rocky mountain spotted fever (rmsf) is an unusual but important dermatological condition to identify without hesitation. the classic triad of headache, fever, and a rash that begins on the extremities and travels proximally to involve the trunk is found in a majority of patients. the cutaneous centripetal pattern is a result of cell to cell migration by the causative organism rickettsia rickettsii. such individuals should receive prompt antimicrobial therapy and supportive care to avoid serious ... | 2006 | 16643138 |
| fatal human infection with rickettsia rickettsii, yucatán, mexico. | the first fatal rickettsia rickettsii infection was diagnosed in the southwest of mexico. the patient had fever, erythematous rash, abdominal pain, and severe central nervous system involvement with convulsive crisis. the diagnosis of r. rickettsii infection was established by immunohistochemistry and specific polymerase chain reaction. | 2006 | 16704818 |
| lymph node hemophagocytosis in rickettsial diseases: a pathogenetic role for cd8 t lymphocytes in human monocytic ehrlichiosis (hme)? | human monocytic ehrlichiosis (hme) and rocky mountain spotted fever (rmsf) are caused by ehrlichia chaffeensis and rickettsia rickettsii, respectively. the pathogenesis of rmsf relates to rickettsia-mediated vascular injury, but it is unclear in hme. | 2006 | 16859547 |
| infection of human endothelial cells with spotted fever group rickettsiae stimulates cyclooxygenase 2 expression and release of vasoactive prostaglandins. | rickettsiae, a diverse group of obligately intracellular gram-negative bacteria, include etiologic agents of the spotted fever and typhus groups of diseases. rocky mountain spotted fever and boutonneuse fever, due to rickettsia rickettsii and r. conorii, respectively, are characterized by widespread infection of the vascular endothelium, microvascular injury, and vasculitis. cultured human endothelial cells (ec) are highly susceptible to infection and respond by altering the expression of adhesi ... | 2006 | 16926398 |
| tick-borne rickettsial pathogens in ticks and small mammals in korea. | in order to investigate the prevalence of tick-borne infectious agents among ticks, ticks comprising five species from two genera (hemaphysalis spp. and ixodes spp.) were screened using molecular techniques. ticks (3,135) were collected from small wild-caught mammals or by dragging/flagging in the republic of korea (rok) and were pooled into a total of 1,638 samples (1 to 27 ticks per pool). from the 1,638 tick samples, species-specific fragments of anaplasma phagocytophilum (1 sample), anaplasm ... | 2006 | 16957192 |
| myocardial involvement in rocky mountain spotted fever: a case report and review. | rocky mountain spotted fever (rmsf), caused by rickettia rickettsii, is a serious tickborne illness that is endemic in the southeastern united states. although it is most commonly known as a cause of fever and rash, it can have systemic manifestations. the myocardium may rarely be involved, with symptoms that can mimic those of acute coronary syndromes. this report describes a case of serologically proven rmsf causing symptomatic myocarditis, manifested by chest pain, elevated cardiac enzyme lev ... | 2006 | 17031247 |
| brazilian spotted fever: a case series from an endemic area in southeastern brazil: epidemiological aspects. | brazilian spotted fever (bsf) is the most important tick-borne disease in brazil and is caused by rickettsia rickettsii and transmitted by the ixodid tick amblyomma cajennense, its main vector. we present epidemiologic aspects of a case series of patients admitted to the hospital das clínicas da unicamp from 1985 to 2003 with a confirmed diagnosis of bsf either by a fourfold rise in indirect immunofluorescence (ifa) titers of igg antibodies reactive with r. rickettsii or isolation of r. ricketts ... | 2006 | 17114702 |
| fatal case of brazilian spotted fever confirmed by immunohistochemical staining and sequencing methods on fixed tissues. | the authors describe the first characterization of rickettsia rickettsii in a fatal case occurring in rio de janeiro state, brazil. | 2006 | 17114718 |
| detection of rickettsia rickettsii and rickettsia sp. in blood clots in 24 patients from different municipalities of the state of sao paulo, brazil. | the authors detected rickettsia genus organisms using shell vial and polymerase chain reaction (pcr)/sequencing analysis in blood clots in patients suspected of having brazilian spotted fever (bsf). dna was detected using pcr with three sets of primers to access the glta, ompa, and ompb genes. sequence analysis was carried out using an automatic sequencer with bioedit software. seventy-five percent of the culture samples were positive and all samples amplified rickettsial gene fragments. to date ... | 2006 | 17114719 |
| a rickettsial mixed infection in a dermacentor variabilis tick from ohio. | we present the first report of superinfection in a dermacentor variabilis tick from nature. the single tick, collected in ohio, was found infected with rickettsia belli, r. nontanensis, and r. rickettsii. | 2006 | 17114734 |
| rocky mountain spotted fever in arizona: documentation of heavy environmental infestations of rhipicephalus sanguineus at an endemic site. | a recent epidemiologic investigation identified 16 cases and 2 deaths from rocky mountain spotted fever (rmsf) in two eastern arizona communities. prevalence studies were conducted by collecting free-living ticks (acari: ixodidae) from the home sites of rmsf patients and from other home sites within the community. dry ice traps and flagging confirmed heavy infestations at many of the home sites. only rhipicephalus sanguineus ticks were identified and all developmental stages were detected. it is ... | 2006 | 17114735 |
| molecular characterization of rickettsia rickettsii infecting dogs and people in north carolina. | rocky mountain spotted fever (rmst) is an important cause of morbidity and mortality in people and dogs in the united states. disease manifestations are strikingly similar in both species, and illness in dogs can precede illness in people. r. rickettsii has been identified as a select agent by the cdc as a category c priority pathogen by the national institute of allergic and infectious diseases because it is amenable to use as a bioterror agent. the clinical and temporal relationship of natural ... | 2006 | 17114748 |
| spotted fever group rickettsial infection in dogs from eastern arizona: how long has it been there? | a serosurvey of free-roaming dogs for antibodies to spotted fever group rickettsiae was conducted using archival samples that had been collected in the white mountain region of eastern arizona during a plague study in 1996. immunoglobulin g antibodies to rickettsia rickettsii (5.1%) and to r. rhipicephali (3.6%) were demonstrated, and no cross-reactive samples were identified. this study indicates that r. rickettsii was likely present in the dog populations in this area prior to the recognition ... | 2006 | 17114769 |
| isolation of rickettsia rickettsii and rickettsia bellii in cell culture from the tick amblyomma aureolatum in brazil. | brazilian spotted fever (bsf) is a highly lethal disease caused by rickettsia rickettsii. in the present study, rickettsial infection was evaluated in 669 amblyomma aureolatum adult ticks collected from naturally infested dogs in taiaçupeba, a bsf-endemic area in the state of são paulo. ten (1.49%) ticks were infected with rickettsia bellii, and 6 (0.89%) ticks were infected with r. rickettsii. both rickettsia species were isolated and established in vero cell cultures. the rickettsia isolates w ... | 2006 | 17114770 |
| molecular typing of novel rickettsia rickettsii isolates from arizona. | seven isolates of rickettsia rickettsii were obtained from a skin biopsy, two whole-blood specimens, and from rhipicephalus sanguineus ticks from eastern arizona. molecular typing of seven isolates of r. rickettsii and dna samples from two other rh. sanguineus ticks infected with r. rickettsii was conducted by pcr and dna sequencing of rompa and 12 variable-number tandem repeat regions (vntrs). all dna specimens from arizona were identical to each other and to reference human and dermacentor and ... | 2006 | 17114781 |
| [study on the development of a real-time quantitative polymerase chain reaction assay to detect rickettsia]. | to develop a real-time quantitative polymerase chain reaction(pcr) assay for detecting rickettsia rickettsii. | 2006 | 17152516 |
| [new trends in clinical neurophysiology of the spinal cord (review of the literature)]. | 2006 | 1665637 | |
| myocardial entrapment by lymphoma: a cause of reversible segmental left ventricular dysfunction. | 2006 | 1636600 | |
| in situ activation of syngeneic tumour-specific cytotoxic t lymphocytes: intra-pinna immunization followed by restimulation in the peritoneal cavity. | tumour-specific cytotoxic t lymphocytes (ctl) are usually obtained after immunization in vivo and restimulation of immune cells in vitro. we here describe the generation of syngeneic tumour-specific ctl within no more than 9 days by priming and restimulation in vivo. this is achieved only if the correct sites are used both for primary immunization (ear pinna) and for restimulation (peritoneal cavity). the kinetics of immune t cell induction and of the secondary response in vivo will be reported. ... | 2006 | 1868488 |