Publications

homologous induction of growth hormone receptors in cultured rat hepatocytes.	the administration of growth hormone (gh) to animals has been reported to induce gh receptors in liver and adipose tissue. however, gh addition to cultured fibroblasts and lymphoblasts downregulated gh receptors, suggesting an indirect mechanism for gh upregulation of its receptors in vivo. we evaluated the direct role of gh by adding it to rat hepatocytes cultured in serum-free medium supplemented as previously described (barash et al. (1988) endocrinology 122, 1151-1158). after 3 days in cultu ...	1989	2744229
phylogenetic diversity of the rickettsiae.	small subunit rrna sequences have been determined for representative strains of six species of the family rickettsiaceae: rickettsia rickettsii, rickettsia prowazekii, rickettsia typhi, coxiella burnetii, ehrlichia risticii, and wolbachia persica. the relationships among these sequences and those of other eubacteria show that all members of the family rickettsiaceae belong to the so-called purple bacterial phylum. the three representatives of the genus rickettsia form a tight monophyletic cluste ...	1989	2753854
[herpesvirus and acute peripheral facial paresis].		1989	2756590
protein synthesis and degradation in non-cultured and in-vitro cultured rabbit blastocysts.	in 'pulse-chase' experiments synthesis and half-lives of leucine-labelled proteins were determined in rabbit blastocysts. embryos were either non-cultured controls or were cultured for 24 h or 48 h in ham's f-10 medium supplemented with homologous serum or uterine flushings. in control blastocysts protein synthesis increased by a factor of 10 between day 4 and day 5. half-lives of newly synthesized proteins were 32 h in day-4 and 99 h in day-5 control blastocysts. in-vitro culture of day-4 blast ...	1989	2760880
extracellular space in the developing retina assessed by electron microscopy: laminar and topographic distribution.	the extent of extracellular space (ecs) in the developing retina of the cat has been measured by electron microscopy in material fixed using techniques developed by others to preserve ecs. ecs is generally greater in foetal than in adult material. it is particularly marked in the plexiform layers of retina at the time of synaptogenesis and in the axon layer at the time of axon growth. the changes in ecs occur first in the central retina, and spread to the periphery. these observations suggest th ...	1989	2761786
primidone/phenobarbital-induced periodic alternating nystagmus.	a 37-year-old man with a history of seizures developed periodic alternating nystagmus (pan) along with other signs of primidone/phenobarbital toxicity. the pan gradually diminished in cycle length and intensity, finally resolving with gradual discontinuation of the drugs.	1989	2764437
[prospects for measuring the rheologic characteristics of human soft tissues based on the recording of their transverse rigidity].	it is shown by an example of a forearm that to determine objective characteristics of rheological properties of soft human tissues by measuring their transverse stiffness (k) one should first investigate the relation between k and the diameter of the used indentor (d). the nonlinearity of the dependence k(d) for indentor diameters from 2 to 8 mm is demonstrated and it is assumed that this nonlinearity is due to heterogeneity of tissue properties in the directions normal to the axis of force acti ...	1989	2765581
comparative sequence analysis of a genus-common rickettsial antigen gene.	the genes encoding the 17-kilodalton genus-common antigen have been cloned and sequenced from rickettsia conorii, rickettsia prowazekii, and rickettsia typhi. compared with the r. rickettsii sequence, this sequence had a high degree of homology within the coding and control regions (r. conorii, 99.8%; r. prowazekii, 88.1%; r. typhi, 88.7%). the 5' flanking regions, including the promoter and the transcription initiation sites, were extremely well conserved for all four species, suggesting that c ...	1989	2768201
human aldehyde dehydrogenase: coenzyme binding studies.	the binding of nadh and nad+ to the human liver cytoplasmic, e1, and mitochondrial, e2, isozymes at ph 7.0 and 25 degrees c was studied by the nadh fluorescence enhancement technique, the sedimentation technique, and steady-state kinetics. the binding of radiolabeled [14c]nadh and [14c]nad+ to the e1 isozyme when measured by the sedimentation technique yielded linear scatchard plots with a dissociation constant of 17.6 microm for nadh and 21.4 microm for nad+ and a stoichiometry of ca. two coenz ...	1989	2775711
adrenal vein catecholamines and neuropeptides during splanchnic nerve stimulation in cats.	splanchnic nerve stimulation in bursts at low (5 hz) and high (50 hz) frequency (30 v, 1 msec; train duration 1 sec; train rate 0.5/second) was employed in 10 cats under halothane anesthesia, during 10-minute periods, while blood samples were concurrently collected from the adrenal vein and femoral artery for the measurement of norepinephrine (ne), epinephrine (epi), dopamine (da), met-enkephalin (me), neuropeptide y (npy), peptide yy (pyy) and neurotensin (nt). in group i (n = 5), splanchnic ne ...	1989	2780418
the mrna for alpha 1-acid glycoprotein is deadenylated during stimulation by dexamethasone.		1989	2780624
the induction of protective immunity to leishmania major in the balb/c mouse by interleukin 4 treatment.	the ability of interleukin 4 (il4), administered subcutaneously around the cutaneous lesion in the form of hydrophilic gels, to affect the development of established leishmania major infections in the balb/c mouse was studied. il4 had a therapeutic effect on l. major lesion growth compared with control mice, causing not only resolution of the parasite lesions over a period of 10 weeks but also rendering animals resistant to reinfection. adoptive transfer of splenic t cells, obtained from il4-tre ...	1989	2786473
morphological studies of rat lingual filiform papillae.	in an attempt to elucidate the functions of three types of filiform papillae in the rat's tongue, corrosive resin casts of the microvessels in the filiform papillae were observed under the scanning electron microscope. the filiform papillae were also examined by light and transmission electron microscopy. the three dimensional architecture of the microvessel loops in the small conical filiform papillae was relatively simple with limited divergence and anastomosis in the papillae, whereas that in ...	1989	2812683
morphological studies of rat lingual filiform papillae.	in an attempt to elucidate the functions of three types of filiform papillae in the rat's tongue, corrosive resin casts of the microvessels in the filiform papillae were observed under the scanning electron microscope. the filiform papillae were also examined by light and transmission electron microscopy. the three dimensional architecture of the microvessel loops in the small conical filiform papillae was relatively simple with limited divergence and anastomosis in the papillae, whereas that in ...	1989	2812683
heterogeneity of cd4-positive human t-cell clones which recognize the surface protein antigen of rickettsia typhi.	immunity to the typhus group of rickettsiae is largely dependent on the effector function of several classes of t lymphocytes, including those which produce gamma interferon. since the surface protein antigen (spa) derived from typhus group rickettsiae has been shown to be an effective immunogen in animal models, human t-cell clones specific for the spa of rickettsia typhi were isolated and tested for their antigenic specificity, as well as for their ability to produce gamma interferon. eighteen ...	1989	2466795
[detection of antibodies to rickettsia prowazekii using 3 serologic tests].	commercial antigens of r. prowazekii may be used for the diagnosis of the typhus group rickettsiosis by the new solid-phase indirect enzyme immunoassay (spi eia). high sensitivity of the test makes the diagnosis of rickettsioses more effective and ensures an earlier detection of typhus patients. the authors have employed spi eia modification with polyethylene glycol, permitting a change in the incubation regimen (20 degrees c instead of 37 degrees c) and more rapid reaction; this recommends the ...	1989	2467065
differential immunohistochemical localization of cytokeratins and collagen types i and iii in experimentally-induced cirrhosis.	liver cirrhosis was induced in male wistar rats by subcutaneous injection (1 ml of 30 g/l) of an aqueous solution of thioacetamide. using the indirect immunoperoxidase technique, high molecular weight keratins were localized in bile ducts and ductules. low molecular weight cytokeratins were present in regenerating hepatocytes in active cirrhosis; bile ducts were unstained. these results suggest that cytokeratin staining may be useful in distinguishing bile duct epithelium and hepatocytes in hepa ...	1989	2478687
[an indirect solid-phase immunoenzyme method in diagnosing rickettsial infections].	sera of brill-zinsser disease patients (70 samples) q fever patients (12 samples) and healthy persons from q fever enzootic areas (99 samples) were studied in indirect solid phase enzyme immunoassay (elisa), indirect immunofluorescent antibody test (ifa) and indirect passive hemahglutination test. elisa and ifa have been proved as most sensitive tests for detection of rickettsial antibodies in both clinical and retrospective diagnosis of rickettsial diseases.	1989	2485298
[characteristics of monoclonal antibodies directed to species specific and group specific antigens of rickettsia prowazekii].	a number of hybridomas against different determinants of r. prowazekii were obtained by hybridization of splenocytes from balb/c mice immunized with whole cells or soluble antigens of the organism. some of the monoclonal antibodies bound to thermolabile species specific protein of r. prowazekii and did not react with r. mooseri antigens (monoclonal antibodies series b4/4 and a3). monoclonal antibodies c5/2 and a3/2 reacted with group specific thermostabile antigen which is common for both r. pro ...	1989	2485302
liver necrosis caused by mushroom poisoning in dogs.		1989	2488352
phospholipase a activity associated with the growth of rickettsia prowazekii in l929 cells.	cultured l929 cells infected with rickettsia prowazekii had a greatly increased rate of hydrolysis of fatty acid from the oleic acid-radiolabeled phospholipids of the host cell membranes. the incorporation of fatty acid into phospholipid in an infected cell was only moderately inhibited relative to a mock-infected cell. thus, even if the release of fatty acid from phospholipid represented a steady state between hydrolysis and resynthesis of phospholipids, the increase in release of fatty acid wa ...	1989	2491840
permeability of rickettsia prowazekii to nad.	rickettsia prowazekii accumulated radioactivity from [adenine-2,8-3h]nad but not from [nicotinamide-4-3h]nad, which demonstrated that nad was not taken up intact. extracellular nad was hydrolyzed by rickettsiae with the products of hydrolysis, nicotinamide mononucleotide and amp, appearing in the incubation medium in a time- and temperature-dependent manner. the particulate (membrane) fraction contained 90% of this nad pyrophosphatase activity. rickettsiae which had accumulated radiolabel after ...	1989	2492515
comparison of antibody response with delayed hypersensitivity in severely injured patients.	twenty-seven severely injured patients had antibody response to gram-negative organisms measured of whom 25 also had skin testing. twenty-three patients (92%) were anergic at admission. injury severity scores were greater in patients who remained anergic for three weeks compared with patients whose skin tests became positive. patients with major infection had longer anergy duration than uninfected patients. twenty-two (81%) of 27 anergic patients mounted antibody responses. initial bacterial con ...	1989	2493240
isolation of rickettsia prowazekii with reduced sensitivity to gamma interferon.	the growth of rickettsia prowazekii madrid e was monitored in mouse l929 cells subcultured for several weeks in the presence of gamma interferon (ifn-gamma) to determine whether the rickettsiae would be eliminated from or would persist in these cultures. r. prowazekii exhibited two distinct patterns in these ifn-gamma-treated cultures. in some cases, ifn-gamma-induced inhibition of rickettsial growth led to elimination of the rickettsiae from the l929 cell cultures; in other cases, the initial i ...	1989	2498207
[detection of typhi antibodies by latex agglutination].	a latex test for immunodiagnosis of murine typhus, using erythrocyte-sensitizing substance from rickettsia prowazekii absorbed to latex particles, has been developed. the test was evaluated with a total of 234 healthy person and non-typhus patient sera, 89 single and 63 paired typhus patient sera. the positive rate was 92.1% for paired and 80.9% for single sera from patients with typhus. the test gave results consistent with those given by the microimmunofluorescence (micro-if) and microagglutin ...	1989	2500317
roles of the fc receptor and respiratory burst in killing of rickettsia prowazekii by macrophagelike cell lines.	it is known that the virulent strain of rickettsia prowazekii grows in macrophagelike cell lines, but if the rickettsiae are treated with antirickettsial serum before infection, the intracellular rickettsiae fail to grow and are destroyed. the uptake of rickettsiae by macrophagelike cell lines was increased by treatment of the rickettsiae with immune serum and with purified immunoglobulin g (igg) from this serum but not by treatment with the f(ab')2 fragment derived from this igg. this suggested ...	1989	2501218
[electrophoretic and immunochemical characteristics of proteins of rickettsia prowazekii strains of various virulence].	paag-electrophoresis of the isogenic pair of rickettsia prowazekii strains e and evir lysates demonstrate the similarity in polypeptide tracks. the different electrophoretic mobility of the mr 30 kd protein from these strains as compared with the mobility of analogous protein from the standard virulent breinl strain is registered. in immunoblot experiments the specific rabbit antiserums obtained on the 30th day of infection with the breinl, e or evir strains demonstrate the presence of the diffe ...	1989	2501676
[monoclonal autoantibodies to the epithelial basement membrane cells of human skin and thymus obtained through immunization with rickettsia prowazekii antigens].	as the result of immunization of balb/c mice with the commercial preparation of typhus vaccine and r. prowazekii corpuscular antigen, in 29.2% and 40.3% of cases (respectively) the appearance of hybridomas synthesizing monoclonal antibodies (mcab) to different autologous structures (skin and thymic epithelium, cell nuclei, conjunctive tissue structures and vascular endothelium) has been revealed. the mcab under test have proved to be igm-autoantibodies. mcab m-6, active against the basal membran ...	1989	2506721
interferon-gamma- and rickettsia-induced killing of macrophage-like cells is inhibited by anti-rickettsial antibodies and does not require the respiratory burst.	the effects of antibiotics and anti-rickettsial antibodies on the ability of rickettsia prowazekii to kill interferon-gamma (ifn-gamma)-treated macrophage-like raw264.7 cells and the possible involvement of the macrophage respiratory burst in this killing were investigated. rifampicin and chloramphenicol, antibiotics that inhibited rickettsial growth, did not inhibit the ability of the rickettsiae to kill ifn-gamma-treated raw264.7 cells. however, pretreatment of r. prowazekii with anti-ricketts ...	1989	2507662
[activation of rickettsiae prowazekii in the composition of immune complexes by antiglobulin serum].	the antiglobulin serum (ags) against spotted fever immunoglobulin of guinea pigs was studied for its effect on the in vitro created immune complex rickettsiae + antibody in experiments using the procedure of laboratory cultivation of rickettsiae prowazekii according to weigl. the biological activity of the pathogen is shown to be restored under the action of ags. this fact may be used for detecting the antigen + antibody complexes in case of the spotted fever and for studying their role in the r ...	1989	2507882
[inactivation of concentrated biomasses of rickettsia prowazekii].	the methods used for the sparing inactivation of highly concentrated r. prowazekii biomass and for the decrease of its infectious activity are described. these methods are recommended for use in experiments in the field of molecular biology, as well as for disinfection of different materials contaminated with rickettsiae. as conditions for complete inactivation, incubation at 50 degrees c for 1 hour without chemical disinfectants, treatment with 0.5% phenol solution at 30 degrees c for 12 hours ...	1989	2510429
[effect of interferon and its inducers on the immunogenicity of typhus vaccines].	the acceleration of antibody formation in animals immunized with chemical typhus vaccine has been shown to occur under the influence of interferonogens (poly i--poly c, tyloron) and leukocytic interferon in experiments on guinea pigs and monkeys.	1989	2511707
probe directed at a segment of rickettsia rickettsii rrna amplified with polymerase chain reaction.	in an effort to explore a sensitive taxon-specific detection system for bacteria, we sequenced the 16s rrna from two strains of rickettsia rickettsii, five other rickettsiae, and coxiella burnetti to find a probe site unique to r. rickettsii. we then synthesized a 16-mer that hybridized only to the rrna of r. rickettsii. using a primer complementary to a sequence found only in rickettsial rrna, we then generated a cdna. we amplified the probe site in a 180-base segment of the cdna by using the c ...	1989	2512323
[comparison of in vitro susceptibilities of rickettsia prowazekii, r. rickettsii, r. sibirica and r. tsutsugamushi to antimicrobial agents].	the in vitro activities of 16 antimicrobial agents against rickettsia prowazekii (breinl strain), r. rickettsii (bitterroot strain), r. sibirica (atcc no. vr151) and r. tsutsugamushi (gilliam, karp, kato, shimokoshi, kawasaki and kuroki strains) were determined by the cell culture method. tetracycline, demethylchlortetracycline, doxycycline, minocycline, chloramphenicol, kitasamycin and rifampicin were generally effective (mic, 0.005-0.78 micrograms/ml) to all strains tested. quinolones such as ...	1989	2512394
immunization of rats with cholinergic neurons induces behavioral deficits.	we have previously shown that sera from patients with alzheimer's disease (ad) contain a significantly high level of antibodies to the cell bodies (perikarya; pk) but not to the nerve terminals (synaptosomes) of purely cholinergic neurons from the electric fish torpedo. in the present study we examined the effect of repeated immunization of rats with either of these antigens for one year. immunoblot studies revealed that sera of cholinergic pk immunized rats contained a high level of antibodies ...	1989	2519521
seroepidemiological study of infectious mononucleosis in older patients.	in southern tasmania, australia, primary epstein-barr virus infection occurs in adults greater than 30 years of age at a higher frequency (approximately 13% of all cases) than is generally reported for other parts of the world, and approximately 7% of the general population of the region have no antibodies to the virus. epstein-barr virus should not be overlooked as a possible cause of disease in older patients in similar populations elsewhere.	1989	2536757
effects of human pth-related peptide and human pth on cyclic amp production and cytosolic free calcium in an osteoblastic cell clone.	recently, human parathyroid hormone-related peptide (hpthrp) has been purified and its amino acid sequence determined. within the amino-terminal 13 residues of hpthrp, 8 amino acids were found homologous between hpthrp and human pth (hpth). this peptide was reported to stimulate cyclic amp (camp) production in osteoblastic cell lines (umr106 and ros17/2.8). however, whether or not this peptide affects another second messenger, i.e., cytosolic free calcium ([ca2+]i), in osteoblasts has not yet be ...	1989	2546638
nucleotide sequence of the rickettsia prowazekii atp/adp translocase-encoding gene.	the rickettsia prowazekii atp/adp translocase (tlc) gene (tlc), previously cloned in escherichia coli was localized to a 1.6-kb chromosomal fragment. nucleotide sequence analysis of this fragment revealed an open reading frame of 1494 bp that could encode a hydrophobic protein of 497 amino acids (aa) with an mr of 56,668. analysis of the deduced aa sequence revealed that it contained twelve potential membrane-spanning regions. comparisons between the deduced aa sequence of the r. prowazekii atp/ ...	1989	2555259
assessment of pancreatic islet-cell population in the hyperglycemic athymic nude mouse: immunohistochemical, ultrastructural, and hormonal studies.	previous studies in diabetic animal models have demonstrated altered pancreatic islet-cell populations. to further characterize the diabetic syndrome in our athymic nude mouse colony, we studied the population of endocrine cells in pancreatic islets of 4-week-old normoglycemic and 8-week-old hyperglycemic athymic nude (nu/nu) mice using immunohistochemistry, morphometry, and electron microscopy. in normoglycemic 4-week athymic nu/nu mice, the proportions of b (insulin-secreting) cells and a (glu ...	1989	2569196
methods for purification of rickettsia prowazekii separated from the host tissue: a step-by-step comparison.	two methods for purification of rickettsia prowazekii strains e, e vir, and breinl grown in chick embryo yolk sacs are described. these methods combine either differential centrifugation or sucrose mix, centrifugation through sucrose cushion, 10 mmol/l mgcl2 treatment, filtration through a glass filter ap-20 and 2 cycles of verografin discontinuous density gradient centrifugation. the purification procedure including sucrose mix allowed to recover about 38-42% biologically active rickettsiae, a ...	1989	2574944
restriction endonuclease analysis of the dna of rickettsia prowazekii vaccine strain e and its revertant.	the dna of rickettsia prowazekii vaccine strain e was analysed by restriction analysis with 17 endonucleases in comparison with its virulent revertant - evir and the virulent reference strain breinl. the dna of cloned and uncloned strains showed identical restriction endonuclease patterns. in spite of stable differences in virulence, strains e and evir displayed a totally identical dna cleavage pattern indicating the absence of marked structural differences between their genomes. on the other ha ...	1989	2576585
[entomologic situation regarding arbovirus circulation in the guajaibon area].	the collections of culicidae made in the guajaibón area, north coast of havana province, are analyzed with arbovirologic purposes. the predominance of aedes scapularis in the human bait collections is confirmed. the possibility of arbovirus circulation in the area is suggested.	1989	2577010
mesenteric vascular occlusion and varices complicating midgut malrotation.	long-standing complete obstruction of the superior mesenteric artery and vein and recurrent gastrointestinal bleeding from varices within the bowel were encountered in a child as a complication of midgut malrotation and volvulus. this condition is unusual, but should be considered in cases of gastrointestinal bleeding, or when small bowel biopsy is contemplated for the evaluation of diarrhea or malabsorption in cases of malrotation.	1989	313354
[facial sinuses. radioclinical problems].		1989	209662
focal nodular hyperplasia of the liver--nucleographic and ultrasonic findings.		1989	210191
stage-related, cell-mediated cytotoxicity with effector cell analysis and computation of cytotoxic activity of t cells and non-t cells.		1989	313261
serum cholinesterase activity following anaesthesia.		1990	526884
q-t prolongation in acute pneumothorax.		1990	313650
[prospects of using controlled-action lactoglobulins in the treatment of acute intestinal infections and dysbacteriosis in infants].	the author summarizes the results of the preclinical study and clinical trial of the new biological drug lactoglobulin having an +anti-coli-proteus action. shows that the drug holds promise in the treatment of infants with acute intestinal infections and dysbacteriosis as well as in the enrichment of lactic mixtures used for artificial feeding of premature children and babies referred to the risk group.	1990	2075046
pharmacokinetics and tissue concentrations of cefuroxime.	cefuroxime is well-absorbed and distributed over the human body. this review gives a general summary of published information on tissue concentrations of cefuroxime after an intravenous or intramuscular dose of 750 or 1,500 mg of cefuroxime. tissue concentrations are given for blister fluid, meninges, eyes, respiratory tract, sputum and bronchi, abdominal and urogenital tract, uterine, ovarian and fallopian tube tissue, myometrium, membranes, placenta, puruloid and healthy human milk, bile, pros ...	1990	2075076
homocysteic acid as an endogenous excitatory amino acid.		1990	2080552
[experimental infection index of anopheles sinensis and melanization of periodic brugia malayi larvae].	this paper deals with the infection index of anopheles sinensis infected with the blood meals of 9 different microfilarial densities (5-300 mf/10 microliters) of periodic brugia malayi. the results indicated that the mean number of microfilariae (mf) ingested by mosquitoes increased with the mf-density of the blood meal. the infection rates of vectors were 30, 65, 93 and 100%, when mf-densities were 5, 10, 20 and 50 mf/10 microliters respectively. although the infection rate was 100% when mf-den ...	1990	2099254
diagnosis of acute typhus infection using the polymerase chain reaction.	the first use of the polymerase chain reaction (pcr) for the diagnosis of an acute rickettsial infection is described. a primer pair derived from the 17-kda antigen sequence of rickettsia rickettsii gave specific amplification of a 434-base pair dna fragment from the genome of rocky mountain spotted fever and endemic and epidemic typhus. the assay could detect as few as 30 rickettsiae. detection of pcr-amplified dna with a nonradioactive dna probe confirmed an acute infection with rickettsia pro ...	1990	2108225
[methods of isolation and polypeptide composition of membrane fractions of rickettsia prowazekii].	the methods of cell lysis by lysozyme in tris-edta-sucrose with the consequent disruption of spheroplasts by the osmotic shock were used to obtain the total membranes from the intact or temperature-inactivated rickettsia prowazekii. detergents solubilization methods were used for analysis of outer membrane proteins. sarcosyl insoluble material is shown to contain the main 134, 31, 29.5 and 25 kd proteins, the minor 78, 60, 42, 17 kd proteins, while the mixture of both membranes possess a more co ...	1990	2114532
[isolation and primary characteristics of the common species-specific outer membrane protein of rickettsia prowazekii].	common species-specific protein is isolated for the first time in the chromatographically pure state from the outer membrane of rickettsia prowazekii, and its amino acid composition is determined. as revealed by chromatofocusing technique, the protein possesses pi 4.18 +/- 0.03. three basic ninhydrin-positive compounds, differing from usual amino acids, were discovered in the protein hydrolyzate. data suggesting the subunit structure of the isolated protein are presented.	1990	2115783
interferon-alpha/beta and rickettsia prowazekii: induction and sensitivity.	rickettsia prowazekii madrid e established persistent infections in cultures of growing l-929 cells. although some l-929 cells died, the cultures survived, remained infected with rickettsiae, and continued to grow. r. prowazekii madrid e also induced interferon in l-929 cell cultures, and this interferon modulated rickettsial growth. production of interferon (anti-viral activity) by cultures of r. prowazekii-infected l-929 cells was directly related to the initial rickettsial infection and was b ...	1990	2116101
evidence supporting the hypothesis that rickettsial virulence factors determine the severity of spotted fever and typhus group infections.		1990	2116102
possible typhus-group infection in new york state: presentation of four suspect cases.	epidemiologic investigations were recently conducted on four cases which were reported in new york state in 1986 and 1987, three of which were within one family. these included hospital chart reviews, case or family interviews, animal trappings, and ectoparasite surveys. serologic tests and immunoblots were performed on blood samples obtained from these patients. all four patients had acute febrile illnesses; two required hospitalization and one died. microimmunofluorescence test results using r ...	1990	2116104
the 17-kilodalton protein antigens of spotted fever and typhus group rickettsiae.		1990	2116105
structural analyses of the 120-kda serotype protein antigens of typhus group rickettsiae. comparison with other s-layer proteins.		1990	2116106
rickettsia prowazekii and thymidylate metabolism. growth in thymidylate synthase-deficient eukaryotic host cells.		1990	2116107
screening the rickettsia prowazekii genome for rpod homologs.		1990	2116109
the influence of halothane, isoflurane, and pentobarbital on cerebral plasma volume in hypocapnic and normocapnic rats.	cerebral blood volume (cbv = cerebral plasma volume [cpv] + cerebral red cell volume [crbcv]) is one determinant of intracranial pressure. in an effort to quantitate the effects of anesthetics and paco2 on cbv, the authors measured cerebral plasma volume (cpv) in normocapnic (paco2 approximately 42 mmhg) and hypocapnic (paco2 approximately 22 mmhg) rats receiving 1 mac doses of isoflurane or halothane, or given an approximately equivalent dose of pentobarbital. all animals were paralyzed, their ...	1990	2118316
selection of alpha/beta interferon- and gamma interferon-resistant rickettsiae by passage of rickettsia prowazekii in l929 cells.	the ability of endogenously produced alpha/beta interferon (ifn-alpha/beta) to inhibit rickettsial growth in infected l929 cell cultures was evaluated by comparing the growth of rickettsia prowazekii madrid e in untreated cultures and cultures treated with anti-mouse ifn (alpha + beta) serum. the endogenously produced ifn was neutralized, and rickettsial growth was enhanced in the antiserum-treated cultures. this inhibitory effect of endogenously produced ifn-alpha/beta was used to select ricket ...	1990	2119343
brill-zinsser: still with us.		1990	2119438
purification and partial characterization of the dna-dependent rna polymerase from rickettsia prowazekii.	the dna-dependent rna polymerase was purified from rickettsia prowazekii, an obligate intracellular bacterial parasite. because of limitation of available rickettsiae, the classical methods for isolation of the enzyme from other procaryotes were modified to purify rna polymerase from small quantities of cells (25 mg of protein). the subunit composition of the rickettsial rna polymerase was typical of a eubacterial rna polymerase. r. prowazekii had beta' (148,000 daltons), beta (142,000 daltons), ...	1990	2120186
acquisition of polyamines by the obligate intracytoplasmic bacterium rickettsia prowazekii.	both the polyamine content and the route of acquisition of polyamines by rickettsia prowazekii, an obligate intracellular parasitic bacterium, were determined. the rickettsiae grew normally in an ornithine decarboxylase mutant of the chinese hamster ovary (c55.7) cell line whether or not putrescine, which this host cell required in order to grow, was present. the rickettsiae contained approximately 6 mm putrescine, 5 mm spermidine, and 3 mm spermine when cultured in the presence or absence of pu ...	1990	2120188
[the effect of antibiotics and immunostimulants on rickettsial persistence in experimental typhus infection].	a model of experimental typhus infection has shown that not only specific protection factors but also nonspecific ones are very important to remove rickettsia prowazekii from macroorganisms. a complex application of antibiotics and drugs increasing immunobiological reactivity of the organism (prodigiosan, methyl uracil) noticeably decreases the rickettsia persistence level in the organism of experimental animals, the indices of specific and nonspecific protection being increased.	1990	2120554
endothelial prostaglandin secretion: effects of typhus rickettsiae.	cultured human umbilical vein-derived endothelial cells were incubated with typhus rickettsiae, and supernatants were examined for the presence of prostaglandins i2 (pgi2) and e2 (pge2). cells incubated with metabolically active rickettsiae secreted significantly more pgi2 and pge2 than did those incubated with buffer alone or with killed rickettsiae. the amount of pgi2 secreted was directly related to the number of hemolytically active rickettsiae present; abolishing rickettsial hemolytic activ ...	1990	2121837
characterization of the gene encoding the protective paracrystalline-surface-layer protein of rickettsia prowazekii: presence of a truncated identical homolog in rickettsia typhi.	the dna sequence of the gene encoding the protective surface protein antigen (spa) of rickettsia prowazekii has been determined. the open reading frame of 4836 nucleotides with promoter and ribosome-binding site is present on a 10.1-kilobase ecori fragment. the encoded carboxyl terminus of the 169-kda protein contains a potential transmembrane region and hydrophilic regions with many lysine and arginine residues potentially accessible to proteolytic cleavage. because the rickettsia-derived spa h ...	1990	2122457
[character of post-translation modification of a common type of outer membrane protein from rickettsia prowazekii].		1990	2128601
proposed guidelines for the clinical evaluation of interleukin-1 inhibitors in the treatment of rheumatoid arthritis.	since the effects of interleukin-1 might contribute to the lesions observed in rheumatoid arthritis. a compound which inhibits this cytokine should be an important addition to the therapeutic agents available for use in this disease. clinical evaluation of such a compound must answer the following questions: is the proposed drug "quick" or "slow" acting on the clinical and/or biological symptoms of inflammation? is the proposed drug able to prevent and/or to stop the cartilage damage in rheumato ...	1990	2151768
[the rickettsial genome studied by dna restriction analysis].	the restriction analysis of 6 rickettsia prowazekii strains with the use of 8 restrictases (cfr13i, ecori, hindiii, mspi, mvai, psti, xhoi, bamhi) has been carried out. in the presence of considerable homology in the restriction pictures of dna in these strains some differences in 1-2 fragments within the range of 8,000-20,000 nucleotide pairs have been established. the strains under study have been divided into two groups according to the character of differences in their restrictograms: the gr ...	1990	2164291
rickettsia prowazekii and atp/adp translocase. analysis of gene fusions encoding beta-galactosidase-atp/adp translocase fusion proteins.		1990	2165754
protective effect of inactivated pasteurella haemolytica bacterin challenged in bovine herpesvirus-1 experimentally infected calves.	formalin-inactivated and sonicated pasteurella haemolytica bacterins were prepared from 1 h cultures of bacterial pellets in rpmi-1640 medium containing 7% fetal calf serum. the bacterial pellets were obtained from logarithmic phase growth of the organism by centrifugation. the protective effect of the vaccine was evaluated in 43 specific-pathogen-free hereford crossbred calves and yearlings in three experiments. cattle were either single vaccinated or boosted via three routes; intratracheally ( ...	1990	2168606
[the dynamic changes in the regional volumetric cerebral blood flow and local vascular reactivity in acute craniocerebral trauma in animals with alloxan diabetes].	the authors studied the condition of regional volumetric cerebral blood flow and local vascular reactivity in experimental mild and moderate craniocerebral trauma in 31 animals with alloxan diabetes and in 29 animals with isolated brain injury. it is shown that alloxan diabetes aggravates cerebral vascular disorders occurring in injury to the skull and brain. vasoactive agents cavinton and xantinol nicotinate promote regression of the changed cerebral blood flow.	1990	2168647
reconstitution of nucleotide excision nuclease with uvra and uvrb proteins from escherichia coli and uvrc protein from bacillus subtilis.	recently, an open reading frame which has a deduced amino acid sequence that shows 38% homology to escherichia coli uvrc protein was found upstream of the aspartokinase ii gene (ask) in bacillus subtilis (chen, n.-y., zhang, j.-j., and paulus, h. (1989) j. gen. microbiol. 135, 2931-2940). we found that plasmids containing this open reading frame complement the uvrc mutations in e. coli. we joined the open reading frame to a tac promoter to amplify the gene product in e. coli and purified the pro ...	1990	2174442
effect of pancreatic proteases on plasma cholecystokinin, secretin, and pancreatic exocrine secretion in response to sodium oleate.	the effect of pancreatic proteases or juice on the sodium oleate-stimulated pancreatic secretion and plasma concentrations of secretin and cholecystokinin in anesthetized rats was investigated. each rat received sodium oleate in a dose of 0.12 mmol.h-1 via a duodenal tube. sodium oleate infusion significantly increased pancreatic secretion (volume and protein output) compared with the saline given the control group. the increase in pancreatic secretion paralleled significant elevations of plasma ...	1990	2186956
mutations in the trfa replication gene of the broad-host-range plasmid rk2 result in elevated plasmid copy numbers.	mutated forms of trfa, the replication protein gene of plasmid rk2, that support a minimal rk2 origin plasmid in escherichia coli at copy numbers up to 23-fold higher than normal have been isolated. six such high-copy-number (copy-up) mutations were mapped and sequenced. in each case, a single base transition led to an amino acid substitution in the trfa protein primary sequence. the six mutations affected different residues of the protein and were located within a 69-base-pair region encoding 2 ...	1990	2193921
[nmr-microscopic studies of the condylar cartilage of the temporomandibular joint].	the objectifying of growth mechanisms of the tmj is very important for orthodontic diagnosis, treatment as well as clinical research. condylar cartilage of the tmj from neonatal pigs was investigated on in vivo preparations by nmr-microscopy. nmr-micro-pictures and histologic slide preparations of condylar cartilage were compared and discussed in connection with its implications for treatment with functional appliances.	1990	2227743
[effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog].	a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ...	1990	2238764
human gastric mucosa expresses glandular m3 subtype of muscarinic receptors.	five subtypes of muscarinic receptors have been distinguished by pharmacological and molecular biological methods. this report characterizes the muscarinic subtype present in human gastric mucosa by radioligand binding studies. the receptor density was 27 +/- 6 fmol/mg protein and the tritiated ligand n-methylscopolamine had an affinity of (kd) 0.39 +/- 0.08 nm (n = 11). the m1 receptor selective antagonist pirenzepine and the m2 receptor selective ligand af-dx 116 had low affinities of 148 +/- ...	1990	2253531
in vitro and in vivo anticancer activity of mitozolomide and sparsomycin in human tumor xenografts, murine tumors and human bone marrow.	the colony formation in agar of human tumor xenografts, of murine tumors and of human bone marrow was used as a test system to determine the in vitro activity of the two novel cytostatic agents, mitozolamide and sparsomycin. mitozolomide was additionally studied in vivo in nine human tumor xenografts. the comparison of in vitro/in vivo activity allows an assessment of the relevant in vitro dose based on in vivo pharmacological behavior of a compound. both compounds showed clear dose/response eff ...	1990	2254373
photoperiodic responsiveness in house mice.	house mice (mus musculus) and laboratory strains of rats (rattus norvegicus) have been traditionally considered nonphotoperiodic because their reproductive systems are unaffected by day length (photoperiod). in rats, however, at least three experimental manipulations, perinatal testosterone injection, chronic peripubertal testosterone exposure, or peripubertal olfactory bulbectomy, have revealed latent reproductive photoperiodism. the effectiveness of these experimental treatments may be unique ...	1990	2267249
photoperiodic responsiveness in house mice.	house mice (mus musculus) and laboratory strains of rats (rattus norvegicus) have been traditionally considered nonphotoperiodic because their reproductive systems are unaffected by day length (photoperiod). in rats, however, at least three experimental manipulations, perinatal testosterone injection, chronic peripubertal testosterone exposure, or peripubertal olfactory bulbectomy, have revealed latent reproductive photoperiodism. the effectiveness of these experimental treatments may be unique ...	1990	2267249
lipid soluble antioxidants preserve rabbit corneal cell function.	the survival time of rabbit corneal endothelial cells in isolated perfused cornea is extended dramatically by the addition of oxygen free radical scavengers. lipid soluble antioxidants were found to be more effective than water soluble non-enzymatic antioxidants in extending the survival time. the ultrastructural appearance of endothelial cells treated with vitamin e was compared with controls. vitamin e treated endothelial cells retained normal appearance up to 12h, but without vitamin e, break ...	1990	2335108
rapid analysis of mouse-hamster hybrid cell lines by in situ hybridization.	in situ hybridization techniques for analyzing the murine dna complement of mouse-hamster hybrid cells are described. total genomic mouse dna is labeled with biotin and hybridized without suppression to metaphase spreads from a mouse-hamster hybrid line containing the mouse fusion chromosome x12. detection via fluorochrome-conjugated avidin reveals mouse chromosomal dna with high sensitivity and permits the identification of both normal and aberrant murine chromosomes. conversely, biotinylated t ...	1990	2335353
[monoclonal antibodies in the diagnosis of paratrachoma].	the efficacy of chlamyset antigen (orion, finland) in the diagnosis of paratrachoma was studied; this test system is based on immunofluorscent method with anti-chlamydial monoclonal antibodies. examinations of scrapings off the conjunctiva, obtained from 120 patients with suspected paratrachoma, have demonstrated that in contrast to the routine cytoscopic method for detection of rickettsia prowazekii, this test system improves the efficacy of etiologic diagnosis of paratrachoma by 22.1 percent.	1990	2368252
cloning and nucleotide sequence of a cdna encoding the antifungal-protein of aspergillus giganteus and preliminary characterization of the native gene.		1990	2374718
diagnosis of acute typhus infection using the polymerase chain reaction.	we used the polymerase chain reaction (pcr) for the diagnosis of an acute rickettsial infection. a primer pair derived from the 17-kda antigen sequence of rickettsia rickettsii amplified specifically a 434-bp dna fragment from the genome of rocky mountain spotted fever and endemic and epidemic typhus. the assay was able to detect as few as 30 rickettsiae. detection of pcr-amplified dna with a digoxigenin-labeled dna probe confirmed an acute human infection with rickettsia prowazekii.	1990	2378469
infection with human t cell leukaemia/lymphoma virus type i in patients attending an antenatal clinic in london.		1990	2390591
poor blood glucose regulation predicts sleep and memory deficits in normal aged rats.	poor glucose regulation predicts memory deficits in individual elderly humans. the present experiment determined whether glucose regulation was also related to memory and to sleep in aged rodents. glucose regulation, inhibitory avoidance, and daytime sleep were assessed in young (3-month-old) and old (24-month-old) rats. correlations were obtained between glucose regulation and the other variables in individual rats. in old rats, the magnitude of increases in blood glucose levels after glucose i ...	1990	2394910
properties in culture and persistence in cotton rats of the rickettsia prowazekii vaccine strain e and its mutants.	cultural properties and the capacity for persistence were studied in spontaneous erythromycin-resistant (e errsm), in induced erythromycin-resistant (e erri) mutants and in a virulent revertant (e vir) of the vaccine strain e, as compared with parent vaccine strain e and standard virulent strain breinl of rickettsia prowazekii. cultural properties of the strains were found to differ in passages in chick embryos (ce) and cultures of fl cells. multiplication indices in ce of mutant e erri were sig ...	1990	1975978
immunological characterization of lipopolysaccharides from proteus strains used in weil-felix test and reactivity with patient sera of tsutsugamushi diseases.	immunological analyses of lipopolysaccharides (lps) isolated from proteus strains ox2, ox19, and oxk used as antigens of weil-felix (wf) test, were performed by quantitative agglutination, enzyme-linked immunosorbent assay (elisa), and immunoblotting. antisera against lps and whole cells (wc) of the three proteus strains reacted with homologous lps but not with heterologous lps, and the reaction was inhibited by the o-polysaccharide fraction isolated from the homologous lps except ox19-lps, whic ...	1990	1693170
[a simple method for counting rickettsia cells].	a simple modification of the method for counting rickettsiae is described. the escherichia coli cells (ecc) which served as reference particles were stained in suspension with methylene blue mixed with rickettsia prowazekii (rp) and quickly sprayed over the glass slide. after fixation the samples were stained according to the technique of gimenez and examined in the light microscope under oil immersion. through a grid in the eye-piece it was not so difficult to count red-coloured rp and dark-blu ...	1990	1693751
effect of ammonia on brain serotonin metabolism in relation to function in the portacaval shunted rat.	four weeks following portacaval anastomosis (pca) in the rat, severe liver atrophy, sustained hyperammonemia, and increased plasma and brain tryptophan are observed. administration of ammonium acetate (nh4ac) to rats with pca precipitates severe signs of hepatic encephalopathy (he) (loss of righting reflex progressing to loss of consciousness and ultimately deep coma). to evaluate the relationship between the deterioration of neurological status in he and serotonin (5-ht) metabolism, the levels ...	1990	1693945
measurement of the human immune response to meningococcal lipooligosaccharide antigens by using serum to inhibit monoclonal antibody binding to purified lipooligosaccharide.	we developed a human inhibition monoclonal enzyme-linked immunosorbent assay (himelisa) to investigate the human immune response to the lipooligosaccharides (los) of neisseria meningitidis. monoclonal antibodies (mab) were used to define seven epitopes on four los molecules of a meningococcal strain (126e) previously shown to express immunogenic los epitopes. the assay could distinguish epitope-specific antibody within whole sera. neither the specificity nor the amount of the antibody measured b ...	1990	1694820
[monoclonal antibodies to the species-specific and group antigens of rickettsia prowazekii].	a number of hybridomas to different r. prowazekii determinants were obtained by the hybridization of spleen cells of balb/c mice immunized with r. prowazekii corpuscular and soluble antigens. some of the monoclonal antibodies (mcab) reacted with r. prowazekii thermolabile species-specific protein and did not react with r. typhi antigens (mcab of batches b4/4 and a-d3). mcab c5/2 and a3/2 reacted with the group thermostable antigen, common for r. prowazekii and r. typhi. mcab to the species-speci ...	1990	1695051
[the surface antigens of rickettsia prowazekii studied with monoclonal antibodies].	r. prowazekii antigens have been tested with the use of monoclonal antibodies (mcab) to different epitopes of the microorganism. as revealed in these tests, mcab b4/4 and a-3/d, active against species-specific thermolabile antigen, interact with protein having a molecular weight of 90-120 kd. mcab c5/2, active against thermostable group antigen common with that of rickettsia typhi, interact with lps-like antigen having a molecular weight of 30 kd. ultrastructural immunochemical studies have reve ...	1990	1696768
[studies on defense effects of recombinant human granulocyte colony-stimulating factor (g-csf) to infections. ii. priming effect for superoxide production by human neutrophil].	in present study, we have investigated superoxide (o2-) production from human neutrophils by recombinant human granulocyte colony-stimulating factor (g-csf) using the microtiter plate for the purpose of being close to the inflammatory site. g-csf by itself did not induce the release of o2- in human neutrophil on either fetal bovine serum (fbs)-coated plate or plate uncoated with fbs, even if neutrophils were exposed for maximum 3 hr. however, the optimal concentration of g-csf (50 ng/ml) was abl ...	1990	1698209