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a case of cat scratch disease masquerading as lymphogranuloma venereum. 199910361925
[seroepidemiology of bartonella henselae infection in hiv-infected patients].bartonella henselae infections are closely related to numerous clinical infections of growing interest in spain. since immunosuppressed patients are a potential risk group for infection by this bacteria, the aim of the present was to study the seroepidemiology of b. henselae infection in a risk group (patients with hiv infection) and in a control group (donors).199910614075
presumed ocular bartonellosis.the spectrum of diseases caused by bartonella henselae continues to expand and ocular involvement during this infection is being diagnosed with increasing frequency.199910365031
infected dog and cat bites. 199910366326
recurrent encephalopathy in cat-scratch disease. 199910391197
[cat scratch disease]. 199910635816
chronic bartonella quintana bacteremia in homeless patients.infection with bartonella quintana can cause trench fever, endocarditis, bacillary angiomatosis, and peliosis. an outbreak of bacteremia due to b. quintana has been reported among homeless people in seattle, and the seroprevalence is high among homeless people in both the united states and europe. body lice are known to be the vectors of b. quintana.19999895398
expression of inducible nitric oxide synthase in human granulomas and histiocytic reactions.inducible nitric oxide synthase (inos) is required in immune response against infections and is involved in granuloma formation in animals; in murine macrophages, inos is induced by lipopolysaccharide and interferon-gamma. in contrast, the role of inos in human immune response against infections is still questioned, and its expression in granulomas is poorly investigated. using western blotting and immunohistochemistry, we investigated inos expression in human lymph nodes with nonspecific reacti ...19999916929
the mutt motif family of nucleotide phosphohydrolases in man and human pathogens (review).human cells express at least eight members of the mutt motif protein (or nudix hydrolase) family. these enzymes are believed to eliminate toxic nucleotide derivatives from the cell and regulate the levels of important signalling nucleotides and their metabolites. six have been fully or partially characterized: i) hmth1 is a nucleoside triphosphatase which restricts at-->cg transversions by specifically degrading the oxidized nucleotide 8-oxo-dgtp; ii) hapah1 preferentially degrades the signallin ...199910373642
conservative management of documented neuroretinitis in cat scratch disease associated with bartonella henselae infection.bartonella henselae has been identified as the causative agent of the neuroretinitis associated with cat scratch disease (csd). immunofluorescent antibody tests with good sensitivity and specificity are available to aid in diagnosis. despite diagnostic advances, optimal management remains controversial. we present a case of documented b. henselae macular neuroretinitis managed without antibiotics and discuss antibiotic use in this condition.199910379716
diagnosis of rickettsial diseases using samples dried on blotting paper.the use of filter paper is an inexpensive and convenient method for collecting, storing, and transporting blood samples for serological studies. in addition, samples occupy little space and can be readily transported without refrigeration. rickettsial diseases often evolve according to an epidemic mode and are now considered reemerging diseases, especially in developing countries, under conditions where fieldwork could be difficult. the suitability of collecting whole-blood specimens on filter p ...199910391847
[bartonellosis. ii. other bartonella responsible for human diseases].in addition to bartonella henselae, five other bartonella species were involved in human pathology. as for b. henselae, ectoparasites seem to be responsible for the transmission of most or all these bacterial species. b. bacilliformis is responsible for carrion's disease that occurs in some valleys of colombia, ecuador and peru. this disease is transmitted by biting of infected sandflies. the bacterial reservoir is constituted by humans only. that disease occurs either as an acute form with seve ...19999920964
seroprevalence of bartonella henselae among hiv-1 infected patients in japan. 199910655688
identification of bartonella-specific immunodominant antigens recognized by the feline humoral immune system.the seroreactivities of both naturally and experimentally infected cats to bartonella henselae was examined. serum samples collected weekly from nine cats experimentally infected with b. henselae lsu16 were tested by enzyme-linked immunosorbent assay (elisa) and western blot analysis. the magnitude and isotype of the antibody response were investigated by elisa. western blot analysis allowed the identification of at least 24 bartonella-specific antigens recognized by the cats during infection. a ...199910391863
prevalence of bartonella henselae and bartonella clarridgeiae in an urban indonesian cat population.we studied evidence of bartonella henselae and bartonella clarridgeiae infection in 54 cats living in jakarta, indonesia. by using an indirect immunofluorescence assay, we found immunoglobulin g antibody to b. henselae in 40 of 74 cats (54%). the blood of 14 feral cats was cultured on rabbit blood agar plates for 28 days. bartonella-like colonies were identified as b. henselae or b. clarridgeiae by using restriction fragment length polymorphism analysis and direct sequencing of the pcr amplicons ...19999874661
development and evaluation of a polymerase chain reaction assay using the 16s rrna gene for detection of eperythrozoon suis infection.the 16s ribosomal rna (rrna) gene of eperythrozoon suis was amplified using gene-specific primers developed from genbank sequence accession u88565. the gene was subsequently cloned and sequenced. based on these sequence data, 3 sets of e. suis-specific primers were designed. these primers selectively amplified 1394, 690, and 839 base-pair (bp) fragments of the 16s rrna gene from dna of e. suis extracted from the blood of an experimentally infected pig during a parasitemic episode. no polymerase ...199910353353
rats of the genus rattus are reservoir hosts for pathogenic bartonella species: an old world origin for a new world disease?bartonella species were isolated from the blood of 63 of 325 rattus norvegicus and 11 of 92 rattus rattus from 13 sites in the united states and portugal. infection in both rattus species ranged from 0% (e.g., 0/87) to approximately 60% (e.g., 35/62). a 337-bp fragment of the citrate synthase (glta) gene amplified by polymerase chain reaction was sequenced from all 74 isolates. isolates from r. norvegicus were most similar to bartonella elizabethae, isolated previously from a patient with endoca ...199910353885
the gene, iala, associated with the invasion of human erythrocytes by bartonella bacilliformis, designates a nudix hydrolase active on dinucleoside 5'-polyphosphates.iala, one of two genes associated with the invasion of human red blood cells by bartonella bacilliformis, the causative agent of several diseases, has been cloned and expressed in escherichia coli. the protein, iala, contains an amino acid array characteristic of a family of enzymes, the nudix hydrolases, active on a variety of nucleoside diphosphate derivatives. iala has been purified, identified, and characterized as an enzyme catalyzing the hydrolysis of members of a class of signaling nucleo ...19999880487
detection and identification of ehrlichia, borrelia burgdorferi sensu lato, and bartonella species in dutch ixodes ricinus ticks.a sensitive and specific pcr hybridization assay was developed for the simultaneous detection and identification of ehrlichia and borrelia burgdorferi sensu lato. in separate assays the 16s rrna gene of ehrlichia species and the 23s-5s rrna spacer region of b. burgdorferi sensu lato were amplified and labeled by pcr. these pcr products were used in a reverse line blot hybridization assay in which oligonucleotide probes are covalently linked to a membrane in parallel lines. hybridization of the s ...199910364588
acute clinical disease in cats following infection with a pathogenic strain of bartonella henselae (lsu16).bartonella henselae is the causative agent of human cat scratch disease as well as several serious sequelae of infections, including bacillary angiomatosis and bacillary peliosis. conflicting reports describe the pathogenesis of b. henselae in the cat. in this study, we characterized a strain of b. henselae termed lsu16. this strain was isolated on rabbit blood agar from a naturally infected 10-month-old female cat during a recurrent episode of bacteremia. the bacterial species was confirmed by ...199910338522
culture-positive bartonella quintana endocarditis.a 50-year-old alcoholic man with a 1-year history of pyrexia of unknown origin was admitted with symptoms of endocarditis. large vegetations on his mitral and aortic valve were found on echocardiography. using the bactec 9240 system. bartonella quintana could be grown from two culture sets of blood collected before the start of antimicrobial therapy. this is the first case report of culture-proven bartonella infection in the uk. and is noteworthy because bartonella quintana was detected in conve ...199910219581
[three children with systemic cat scratch disease].three girls with systemic cat scratch disease, aged 10, 13 and 9 years, were reported. they presented a prolonged fever and back pain in the early stage of the disease, and had no regional lymphadenopathy. two of them had hepatosplenic granulomas, one with multiple 5 mm hypoechoic lesions in the liver and spleen, and the other with a single 2.5 cm hypodense lesion in the left hepatic lobe. the latter patient underwent a partial left hepatic lobectomy. all patients had elevated titers of antibodi ...199910222672
use of polymerase chain reaction for citrate synthase gene to diagnose bartonella quintana endocarditis.we describe aortic valve endocarditis caused by bartonella quintana in a 31-year-old man. the diagnosis was made on the basis of polymerase chain reaction amplification of the b quintana citrate synthase gene from cardiac valve tissue, the compatibility of histochemical stains of cardiac valve tissue, and serologic studies.199910396283
[infection and angiomatous cutaneous lesions].the occurrence of angiomatous cutaneous lesions in the presence of an infective process is not a frequent phenomenon. most infectious diseases are associated with an exanthematous reaction. the combination of an infective illness and angiomatous lesions is seen essentially in the bartonelloses and in kaposi's disease. bartonelloses: group of infections due to alpha-proteobacteria such as bartonella. bartonella bacilliformis (bb), is the causal agent of carrion's disease, the chronic cutaneous fo ...199910399647
systemic bartonella henselae infection with hepatosplenic involvement.systemic manifestations of bartonella henselae infection are rare in the immunocompetent host. the infection generally has initial symptoms of prolonged fever and multiple granulomatous lesions in liver and spleen.199910400104
radiological case of the month. atypical cat-scratch disease. 199910401812
evaluation of intraspecies genetic variation within the 60 kda heat-shock protein gene (groel) of bartonella species.a phylogenetic investigation was done on the members of the genus bartonella, based on the dna sequence analysis of the groel gene, which encodes the 60 kda heat-shock protein groel. nucleotide sequence data were determined for a near full-length fragment (1368 bp) of the groel gene of the established bartonella species and used to infer intraspecies phylogenetic relationships. phylogenetic trees were inferred from multiple sequence alignments by using both distance and parsimony methods, which ...199910425758
development of a system for genetic manipulation of bartonella bacilliformis.lack of a system for site-specific genetic manipulation has severely hindered studies on the molecular biology of all bartonella species. we report the first site-specific mutagenesis and complementation for a bartonella species. a highly transformable strain of b. bacilliformis, termed jb584, was isolated and found to exhibit a significant increase in transformation efficiency with the broad-host-range plasmid pbbr1mcs-2, relative to wild-type strains. restriction analyses of genomic preparatio ...199910427032
an ulcerated nodule associated with lymphadenopathy. 199910456353
cat scratch encephalopathy.cat scratch disease is usually a self-limited illness associated with tender lymph nodes, fever, malaise, and fatigue. lymphadenopathy usually resolves spontaneously within three to four months. cat scratch disease can be atypical as indicated by the presentation of our patient.199910461442
absence of kaposi's sarcoma-associated herpesvirus dna in bacillary angiomatosis-peliosis lesions.bartonella henselae and b. quintana induce an unusual vascular proliferative tissue response known as bacillary angiomatosis (ba) and bacillary peliosis (bp) in some human hosts. the mechanisms of bartonella-associated vascular proliferation remain unclear. although host factors probably play a role, microbial coinfection has not been ruled out. because of the vascular proliferative characteristics noted in both kaposi's sarcoma (ks) and ba and occasional colocalization of ks and ba, the possibi ...199910479179
images in clinical medicine. cat scratch disease. 19999887162
clinical and pathologic evaluation of chronic bartonella henselae or bartonella clarridgeiae infection in cats.human bartonella infections result in diverse medical presentations, whereas many cats appear to tolerate chronic bacteremia without obvious clinical abnormalities. eighteen specific-pathogen-free cats were inoculated with bartonella henselae- and/or bartonella clarridgeiae-infected cat blood and monitored for 454 days. relapsing bacteremia did not correlate with changes in protein profiles or differences in antigenic protein recognition. intradermal skin testing did not induce a delayed type hy ...199910203518
bartonella spp infection as a possible cause of uveitis in a cat.a 6-year-old castrated mixed-breed cat was evaluated because of unilateral anterior uveitis. the cat was seronegative for antibodies to toxoplasma gondii, coronaviruses, and feline immunodeficiency virus, and antigens for felv p27 and cryptococcus neoformans. antibodies to bartonella spp were detected in serum and aqueous humor. the antibody coefficient (c value) for igg antibodies to bartonella spp in the aqueous humor was 4.42; values > 1 suggest ocular production of antibodies and supports a ...199910212684
[trench fever]. 199910201203
[prevalence of serum igg antibody against bartonella henselae in an asymptomatic japanese population]. 199910077908
bartonella henselae infection associated with peripapillary angioma, branch retinal artery occlusion, and severe vision loss.to report atypical clinical features of bartonella henselae neuroretinitis treated with combination antibiotics.199910030575
interactions of bartonella henselae with vascular endothelial cells.the emerging human pathogen bartonella henselae has the remarkable capacity to colonise vascular tissues and to stimulate vasoproliferative tumour growth. although the molecular principle of bacterium-induced neovascularisation (angiogenesis) is still unclear, recent studies have indicated a novel mechanism of endothelial colonisation that involves the formation, engulfment and uptake of a large bacterial aggregate.199910047560
the iala invasion gene of bartonella bacilliformis encodes a (de)nucleoside polyphosphate hydrolase of the mutt motif family and has homologs in other invasive bacteria.the product of the iala invasion gene of bartonella bacilliformis has been expressed as a thioredoxin fusion protein. it is a (di)nucleoside polyphosphate hydrolase of the mutt motif protein family with strong sequence similarity to plant diadenosine tetraphosphate hydrolases. it hydrolyses nucleoside and dinucleoside polyphosphates with four or more phosphate groups, always producing an ntp as one product. diadenosine tetraphosphate (ap4a) is the preferred substrate with a km of 10 microm and a ...199910080922
bartonella quintana in body lice collected from homeless persons in russia. 199910081691
[cat scratch disease]. 199910088350
[bartonellosis]. 199910088493
haemobartonellosis in squirrel monkeys (saimiri sciureus): antagonism between haemobartonella sp. and experimental plasmodium falciparum malaria.a hemotropic parasite of the genus haemo bartonella (rickettsial parasite of the family anaplasmataceae) is responsible for latent asymptomatic infection in colony-born saimiri monkeys. indeed, many of these animals develop a patent haemobartonella infection following splenectomy. such patent parasitism is characterized by an intense haemobartonella parasitemia which peaks between days 12 and 14 after removal of the spleen and then decreases to become undetectable between days 25 and 30. during ...199910092473
[bartonella infection in humans].bartonella bacilliformis: among the 3 species of bartonella known to be human pathogens, b. bacilliformis causes carriun's disease, which manifests an acute phase (oroya fever) and a chronic phase marked by benign skin eruption with wart like macules of vascular origin. until 1993, b. bacilliformis was considered to be the only species in bartonella genus. in 1993, species formally in the rochalimaea genus were designated as bartonella species. bartonella quintana: this species causes trench fev ...199910093604
prevalence of bartonella henselae immunoglobulin g antibodies in singaporean cats.bartonella henselae causes several clinical diseases in humans. the most common infection is the classical cat-scratch disease (csd) occurring in immunocompetent and immunocompromised children exposed to newly acquired kittens. this is the first study of b. henselae infection in singaporean cat population.199910093952
[cat-scratch disease: series of 14 cases. the diagnostic usefulness of serology].disease by cat scratch (csd) is a syndrome characterized by regional adenopathies following cat scratch or bite. despite knowing the etiologic agent of the same, there is still controversy in the diagnosis of the syndrome in daily clinical practice.199910069107
detection of bartonella henselae dna by two different pcr assays and determination of the genotypes of strains involved in histologically defined cat scratch disease.cat scratch disease (csd) is a common cause of subacute regional lymphadenopathy, not only in children but also in adults. serological and molecular studies demonstrated that bartonella henselae is the etiologic agent in most cases of csd. amplification of b. henselae dna in affected tissue and detection of antibodies to b. henselae are the two mainstays in the laboratory diagnosis of csd. we designed a retrospective study and investigated formalin-fixed, paraffin-embedded lymph nodes from 60 pa ...199910074515
body lice as tools for diagnosis and surveillance of reemerging diseases.body lice are vectors of three bacteria which cause human disease: rickettsia prowazekii, the agent of epidemic typhus; bartonella quintana, the agent of trench fever; and borrelia recurrentis, the agent of relapsing fever. a recrudescence of body lice is being observed as the numbers of individuals living under social conditions which predispose individuals to infestation have increased. because this phenomenon may lead to the reemergence of infections transmitted by body lice, we aimed to asse ...19999986818
cat-scratch disease. 19999917765
management of b. henselae neuroretinitis in cat-scratch disease. 19999917766
vaccination of cats against emerging and reemerging zoonotic pathogens.many of the emerging infectious agents that threaten the human population are either directly zoonotic or involve animals, rather than humans, as their primary reservoir in nature. vaccination of animals may be an important consideration for control of some of these diseases, and this review has specifically focused on the concept of vaccinating cats in the prevention of infection with t. gondii, b. henselae, and h. pylori. if we return to the considerations that were presented in table iii, t. ...19999890026
bartonella henselae infection as a cause of fever of unknown origin.fourteen of 41 patients (34%) with a serological diagnosis of bartonella henselae infection were found to have prolonged fever or fever of unknown origin, suggesting that generalized systemic b. henselae infection is not rare in immunocompetent healthy individuals.200010790137
subtyping of uncultured bartonellae using sequence comparison of 16 s/23 s rrna intergenic spacer regions amplified directly from infected blood.this study aimed to assess the usefulness of a pcr-based approach to the detection and differentiation of bartonella strains in infected blood. the conservation of potential genus-specific pcr primer hybridisation sites within the 16 s/23 s rrna gene intragenic spacer regions of bartonella species was confirmed following sequence analysis of the intragenic spacer regions of four previously untested species. the extent of intra-species variation within the specific amplicons was assessed by compa ...200010799268
bartonella interactions with host cells. 200010804853
prevalence of bartonella henselae, bartonella clarridgeiae and the 16s rrna gene types of bartonella henselae among pet cats in japan.the authors investigated bacteriologically the prevalence of bartonella infection among 690 pet cats derived from 10 private animal hospitals in six cities (sapporo, hokkaido prefecture; sendai, miyagi prefecture; joetsu, niigata prefecture; fujisawa, kanagawa prefecutre; kyoto, kyoto prefecture; sanda, hyogo prefecutre) and 4 counties (mishima, osaka prefecture; hikawa, shimane prefecture; aira, kagoshima prefecture; shimajiri, okinawa prefecture) located from the north to the south of japan. b ...200010770599
in vitro activities of telithromycin (hmr 3647) against rickettsia rickettsii, rickettsia conorii, rickettsia africae, rickettsia typhi, rickettsia prowazekii, coxiella burnetii, bartonella henselae, bartonella quintana, bartonella bacilliformis, and ehrlichia chaffeensis.in vitro activities of telithromycin compared to those of erythromycin against rickettsia spp., bartonella spp., coxiella burnetii, and ehrlichia chaffeensis were determined. telithromycin was more active than erythromycin against rickettsia, bartonella, and coxiella burnetii, with mics of 0.5 microg/ml, 0.003 to 0.015 microg/ml, and 1 microg/ml, respectively, but was inactive against ehrlichia chaffeensis.200010770788
clinical disease in kittens inoculated with a pathogenic strain of bartonella henselae.to evaluate disease in kittens inoculated with bartonella henselae strain lsu16.200010772100
visceral manifestation of cat scratch disease in children. a consequence of altered immunological state?a 12-year-old girl with a 2-month history of fever and abdominal pain was admitted to our hospital. ultrasound and ct scans of the abdomen showed multiple hypoechoic lesions of liver and spleen. screening for zoonosis revealed high positive titers to bartonella henselae. t-cell deficiency was demonstrated and remained almost unchanged during a follow-up of 11 months. a review of the literature shows that disseminated visceral affection is a rare presentation of cat scratch disease (csd) in child ...200010782400
bartonella vinsonii subsp. berkhoffii as an agent of afebrile blood culture-negative endocarditis in a human.we report a case of endocarditis in a human infected with bartonella vinsonii subsp. berkhoffii, which causes bacteremia and endocarditis in dogs. bacterial identification was established by pcr amplification and sequencing of an intergenic spacer region (its1), 16s ribosomal dna, and a gene encoding citrate synthase (glta). bartonella antibodies were detected by immunofluorescence.200010747175
parapharyngeal abscess due to cat-scratch disease.the spectrum of illness attributed to cat-scratch disease (csd) continues to expand. although a common cause of cervical adenitis in children, csd has not been associated as a cause of deep fascial space infections of the head and neck. we describe a child with extensive parapharyngeal adenitis and abscesses due to csd confirmed by histological and serological evaluations.200010722453
patterns of infection of haemoparasites in the fat sand rat, psammomys obesus, in tunisia, and effect on the host.two bacterial and one protozoan blood parasite, belonging to the genera bartonella, borrelia and babesia, were studied in a tunisian population of psammomys obesus. seasonal changes in the abundance of the parasites and host were monitored in a longitudinal field survey lasting 17 months. blood samples collected during eight rodent-trapping sessions, between september 1995 and january 1997, were examined microscopically. bartonella sp. showed a seasonal pattern, with most transmission occurring ...200010723524
in vitro susceptibilities of rickettsia and bartonella spp. to 14-hydroxy-clarithromycin as determined by immunofluorescent antibody analysis of infected vero cell monolayers.the in vitro susceptibilities of rickettsia akari, rickettsia conorii, rickettsia prowazekii, rickettsia rickettsii, bartonella elizabethae, bartonella henselae and bartonella quintana to different concentrations of clarithromycin, 14-hydroxy-clarithromycin (the primary metabolite of clarithromycin) and tetracycline in vero cell cultures, were determined by enumeration of immunofluorescently-stained bacilli. the extent of antibiotic-induced inhibition of foci was recorded for each dilution of an ...200010702548
prevalence of bartonella henselae antibody in florida panthers.serum samples from 28 free-ranging florida panthers (puma concolor coryi) and seven mountain lions from texas (p. concolor stanleyana) living in south florida (usa) between 1997 to 1998 were tested for antibodies to bartonella henselae. twenty percent (7/35) of the samples were reactive to b. henselae antisera with a subspecies prevalence of 18% (5/ 28) for florida panthers and 28% (2/7) for cougars from texas (usa). there was not a significant sex related difference in infection rates among the ...200010682759
peliosis hepatis in a dog infected with bartonella henselae.a 6-year-old spayed female golden retriever was examined because of generalized weakness and abdominal distention. abdominal ultrasonography revealed a large quantity of peritoneal fluid. in addition, the liver appeared larger than normal and contained multiple, small, nodular masses and cyst-like structures. abdominal exploratory surgery was performed, and 5 l of serosanguineous peritoneal fluid was removed. gross lesions were not found in the stomach, kidneys, intestines, adrenal glands, or ur ...200010687006
bacillary angiomatosis by bartonella quintana in an hiv-infected patient.bacillary angiomatosis and bacillary peliosis are opportunistic infections caused by bartonella henselae and bartonella quintana, which occur in patients with late-stage infection. we report a case of bacillary angiomatosis in an hiv-infected patient with skin, bone, and probably liver involvement, the identification of the agent (b quintana ) was done by polymerase chain reaction in the skin specimen. the patient had complete regression of all lesions after a 6-month regimen of oral erythromyci ...200010642694
sequence variation in the ftsz gene of bartonella henselae isolates and clinical samples.in a search for methods for subtyping of bartonella henselae in clinical samples, we amplified and sequenced a 701-bp region in the 3' end of the ftsz gene in 15 b. henselae isolates derived from cats and humans in the united states and europe. the ftsz sequence variants that were discovered were designated variants bh ftsz 1, 2, and 3 and were compared with 16s rrna genotypes i and ii of the same isolates. there was no ftsz gene variation in the strains of 16s rrna type i, all of which were bh ...200010655367
an update on human bartonelloses.the data available on bartonella species has increased rapidly in the last decade with 12 new species and subspecies being described and bartonellas being found to be associated with an increasing spectrum of clinical syndromes in immunocompetent and immunocompromised patients. in addition b. weissii is found in cats. this rapid expansion of knowledge has come about with the development of new culture and molecular biology techniques to isolate and identify the organisms. much of the information ...200011317589
quantitative analysis of valvular lesions during bartonella endocarditis.cardiac valve pathology was evaluated in 15 patients with confirmed diagnosis of bartonella endocarditis. ten were infected by bartonella quintana and 5 by bartonella henselae. histologic features of these cases, including fibrosis, calcification, vegetation, pattern of inflammation, and vascularization, were compared with those of valves from 25 cases of non-bartonella endocarditis as controls using a computerized quantitative image analysis. pathologic and immunohistologic testing for localiza ...200011338477
osteomyelitis of the hip joint associated with systemic cat-scratch disease in an adult.reported here is the case of a 29-year-old male with cervical lymphadenopathy, fever and weight loss, followed by acute painful osteomyelitis of the left hip joint due to cat-scratch disease. the diagnosis was established by detection of igg antibodies to bartonella henselae in serum and histologic examination of a lymph node including a positive polymerase chain reaction test. treatment consisted of clarithromycin and cefotiam for 2 weeks. four weeks after discharge, all of the patient's sympto ...200011117643
evidence of bartonella henselae infection in cats and dogs in the united kingdom.sera from cats and dogs in the uk were tested by elisa for antibodies to bartonella henselae. seropositivity was confirmed in 28 of 69 pet cats (40.6 per cent), 33 of 79 feral cats (41.8 per cent) and three of 100 pet dogs. reactivity to specific b. henselae antigens was confirmed by western blotting and demonstrated that consistent antigenic bands were bound by sera from the cats and dogs.200011132671
neuroretinitis: a clinical syndrome of cat-scratch disease.cat-scratch disease is usually a benign self-limited illness, characterized by regional lymphadenopathy lasting between 3 and 6 weeks. the causative organism is bartonella henselae, a small gram-negative rod. between 1 and 2% of patients who contract the illness experience blurred vision, metamorphopsia and scotomas as a result of neuroretinitis, an associated clinical syndrome. the classical clinical findings in cat-scratch neuroretinitis include disc edema and a stellate pattern of exudates in ...200011137430
bacillary angiomatosis in hiv-infected patients--an epidemiological and clinical study.no data were available on the epidemiological and clinical characteristics of bacillary angiomatosis (ba) in germany.200011146343
[disciform keratatis caused by bartonella henselae infection: detection of a rare ocular complication of cat-scratch disease with pcr].cat scratch disease (csd) is a common infectious disease, however its association with a disciforme keratitis is to our knowledge a previously unreported ocular complication.200011146830
bartonella birtlesii sp. nov., isolated from small mammals (apodemus spp.).three strains isolated from apodemus spp. were similar to bartonella species on the basis of phenotypic characteristics. futhermore, genotypic analysis based on sequence analysis of the 16s rrna and glta genes and on dna-dna hybridization showed that the three isolates represented a distinct and new species of bartonella. the name bartonella birtlesii is proposed for the new species. the type strain of b. birtlesii sp. nov. is ibs 325t (= cip 106294t = ccug 44360t).200011155970
determination of the rpob gene sequences of bartonella henselae and bartonella quintana for phylogenic analysis.using the genome walker procedure, which allows pcr amplification of genomic dna using a single gene-specific primer and direct automated sequencing methodology, we obtained the nucleotide sequence of the rna polymerase beta subunit (rpob) from bartonella henselae and bartonella quintana. a phylogenetic tree constructed from these data and other rpob sequences available in genbank is, in part, consistent with those previously derived from 16s rrna gene sequences and confirms the position of bart ...200011191808
cervical lymphadenitis and cat scratch disease (csd): an overlooked disease?cat scratch disease (csd) is a benign disease characterized by regional lymphadenopathy affecting most frequently the head and neck region in children and young adults. in the present paper, the authors describe four cases of csd focusing on clinical history, diagnostic management and therapy. the main germ responsible for this lymph node disease is bartonella henselae. diagnosis is based on history, serology and histological findings. clinical evolution is generally favorable despite the fact t ...200011205454
detection of bartonella henselae and afipia felis dna by polymerase chain reaction in specimens from patients with cat scratch disease.polymerase chain reaction (pcr) amplification and colorimetric identification of amplicons were performed to detect bartonella henselae and afipia felis dna in specimens from patients who were clinically and histologically suspected of having cat scratch disease. pcr products were revealed using 2% ethidium bromide agarose-gel electrophoresis and identified with specific probes in a commercial colorimetric hybridization assay (deia) (gen-eti-k; diasorin, italy). six paraffin-embedded lymph node ...200011205638
[cat-scratch disease].we report a case of fourteen years old boy with cat scratch disease. he was treated at pediatric clinic in january and february 2000. cat scratch disease is benign infectious chronic disease which appears in all ages. it begins after contact with cat and its scratch. yearly incidence of this disease for whole population is 0.77-0.86/100.000. the cause is bacillus bartonella which is also called genus rochalimaea. it is gram negative, mobile, aerobe bacillus with ten flagellas on one pole. our pa ...200011219917
transcriptional activation of the htra (high-temperature requirement a) gene from bartonella henselae.bacterial htra genes are typically activated as part of the periplasmic stress response and are dependent on the extracytoplasmic sigma factor rpoe. a putative promoter region, p1, of the sigma(e)-type heat-inducible promoters has previously been identified upstream of the htra gene of bartonella henselae. further analysis of the htra mrna by primer extension demonstrated that transcription initiates from p1 and a second region downstream of p1. this second promoter region, termed p2, had no seq ...200010992509
failure to detect bartonella henselae infection in synovial fluid from sufferers of chronic arthritis.bartonella henselae causes granulomatous and indolent infection in the immune competent human, and angioproliferation in the context of persistent infection and impaired immunity. this bacterium is found in up to 40% of household cats, from which humans acquire it by either a cat scratch or a bite (hence the name, cat-scratch disease). approximately 5% of australian and us blood donors have serological evidence of past infection, but most associated illnesses are mild or subclinical. a number of ...200011063291
[bacillary angiomatosis of the liver, a suspected ultrasound diagnosis?].infections with bartonella henselae or bartonella quintana present with vasoproliferative lesions in skin and parenchymatous organs in immunocompromised patients. a case report of a 38-year-old patient with hiv infection and hepatitis b surface antigen status is described. the dominant clinical symptoms in our patient were fever and icterus. ultrasonography of the abdomen showed a picture similar to that of liver cirrhosis. irregular (echorich) nodes with hyper-vascularization were suspected to ...200011072674
cat-scratch disease.cat-scratch disease (csd) was first described by debré in 1950, yet the causative bacterial agent of csd remained obscure until 1992, when bartonella (formerly rochalimaea) henselae was implicated in csd by serological and microbiologic studies. bartonella henselae had initially been linked to bacillary angiomatosis (ba), a vascular proliferative disease most commonly associated with long-standing human immunodeficiency virus infection or other significant immunosuppression. bartonella henselae ...200011189710
specific and nonspecific enzymes involved in the catabolism of mononucleoside and dinucleoside polyphosphates.this review concerns enzymes that can degrade nucleoside 5'-tetra- and pentaphosphates (p(4)n and p(5)n) and those that can degrade various dinucleoside polyphosphates (np(3-6)n'). most of these enzymes are hydrolases, and they occur in all types of organisms. certain fungi and protozoa also possess specific np(n)n' phosphorylases. specific p(4)n hydrolases have been demonstrated in mammals and in plants. in yeast, p(4)n and p(5)n are hydrolyzed by exopolyphosphatases. among other hydrolases tha ...200011007995
three cases of cat scratch disease diagnosed by indirect immunofluorescence antibody assay and/or polymerase chain reaction of 16s rrna gene of bartonella henselae.three suspected cases of cat scratch disease were diagnosed by indirect immunofluorescence antibody assay and/or polymerase chain reaction. patient 1 was a 10-year-old female who presented swelling of the right axillary [corrected] lymph nodes with pain and fever. she kept a kitten, and many scratches were observed on her both legs and dorsum manus. antibody titers against bartonella (b.) henselae were 1:32 for igm 3 weeks after the onset of the symptoms and 1:64 for igg 8 weeks after the onset. ...200011193351
optic disk edema associated with peripapillary serous retinal detachment: an early sign of systemic bartonella henselae infection.to describe optic disk edema associated with peripapillary serous retinal detachment as an early sign of systemic bartonella henselae infection.200011020412
ocular bartonellosis.to review recent advances in the basic and clinical biology of bartonella-related eye disease.200011020414
interaction of bartonella henselae with endothelial cells results in rapid bacterial rrna synthesis and replication.bartonella henselae is a slow-growing microorganism and the causative pathogen of bacillary angiomatosis in man. here, we analysed how interaction of b. henselae with endothelial cells might affect bacterial growth. for this purpose, bacterial rrna production and ribosome content was determined by fluorescence in situ hybridization (fish) using rrna-targeted fluorescence-labelled oligonucleotide probes. b. henselae grown on agar plates showed no detectable rrna content by means of fish, whereas ...200011207598
[the niches and pathways of animal pathogens].infectious diseases are not a relict of the past but a topical phenomenon determined by complex evolution of the currently existing and constantly changing microbial agents and their hosts. with regard to abundance of species within the microbial kingdom and rate of its changes and development, it is difficult to predict the role of the microbial factor in mortality of humans and animals. the study and generalization of sequential similarities of microbial virulence factors after the completion ...200011039207
experimental evidence of host specificity of bartonella infection in rodents.a large number of bartonella species and genetic variants were compared for their ability to cause bacteremia in different rodent species: the cotton rat (sigmodon hispidus), white-footed mouse (peromyscus leucopus), balb/c mouse and wistar rat. experimental data supported field observations that host specificity can occur among certain bartonella species and rodent species. bacteremia could only be readily produced in cotton rats or white-footed mice if the strains used for inoculation were ori ...200011038125
bartonella henselae associated uveitis and hla-b27.to investigate the frequency of hla-b27 in patients with presumed bartonella henselae associated uveitis and to describe the clinical characteristics of hla-b27 positive patients with uveitis and presumed ocular bartonellosis (pob).200011004097
cyclic cd8+ lymphopenia in dogs experimentally infected with bartonella vinsonii subsp. berkhoffii.until recently, it was presumed that bartonella vinsonii only infected voles, a species of north american rodents. in april of 1993, however, our laboratory isolated a novel subspecies of b. vinsonii (b. vinsonii subsp. berkhoffii) from the blood of a dog diagnosed with vegetative valvular endocarditis. subsequently, based on a seroepidemiologic survey of dogs from north carolina and virginia presenting for a variety of medical problems, we found evidence supporting a potentially important assoc ...200010889298
bartonella infection in animals: carriership, reservoir potential, pathogenicity, and zoonotic potential for human infection.recent observations have begun to support a role for bartonella spp. as animal as well as human pathogens. bartonella spp. are vector-transmitted, blood-borne, intracellular, gram-negative bacteria that can induce prolonged infection in the host. persistent infections in domestic and wild animals result in a substantial reservoir of bartonella organisms in nature that can serve as a source for inadvertent human infection. the prevalence of bacteremia can range from 50 to 95% in selected rodent, ...200010885985
immune aspects of bartonella.bartonella species have been recognized as important human pathogens only recently. until the early 1990s, this genus was represented by one species, bartonella bacilliformis. the recent identification of other bartonella species as the agents of cat-scratch disease and bacillary angiomatosis has left little doubt of their emerging importance as opportunistic human pathogens. over the last decade, extensive research has been performed on bartonella species, resulting in an explosion in our knowl ...200011052646
[when cats scratch]. 200011059029
recent advances in diagnosis and treatment of cat scratch disease.the cause of cat scratch disease (csd), first described in france in 1950 and in the united states in 1951, was unknown until 1983 when the bacterium in lymph nodes was detected using a warthin-starry silver stain. afipia felis has been an infrequent cause of csd since1988, when this gram-negative bacterium was first isolated from 10 patients with csd. in 1992 bartonella organisms were isolated from immunocompetent and immunocompromised patients. an indirect fluorescent antibody test to detect b ...200011095849
bartonella henselae and inflammatory bowel disease.sustained fever and increased thickness of the distal ileum on ultrasound suggested crohn's disease in an adolescent boy. bartonella henselae infection was diagnosed by specific serology and the patient recovered. ileitis could be related to b. henselae infection.200011072951
coyotes (canis latrans) as the reservoir for a human pathogenic bartonella sp.: molecular epidemiology of bartonella vinsonii subsp. berkhoffii infection in coyotes from central coastal california.bartonella vinsonii subsp. berkhoffii was originally isolated from a dog suffering infectious endocarditis and was recently identified as a zoonotic agent causing human endocarditis. following the coyote bite of a child who developed clinical signs compatible with bartonella infection in santa clara county, calif., this epidemiological study was conducted. among 109 coyotes (canis latrans) from central coastal california, 31 animals (28%) were found to be bacteremic with b. vinsonii subsp. berkh ...200011060089
detection of serum antibodies to bartonella henselae and coxiella burnetii from japanese children and pregnant women.the participation of bartonella henselae and coxiella burnetii in the pathogenesis of fever of unknown origin (fuo) and lymphadenopathy has not been completely clarified. prevalence of these two agents in japanese children is also unknown. serum igg and igm antibodies to b. henselae and to c. burnetii were examined by the indirect fluorescence antibody assay. enzyme immunoassay kits were used to detect serum igg and iga antibodies against chlamydia trachomatis. out of 200 healthy normal pregnant ...200011099929
[neonatal papular acrodermatitis (gianotti-crosti) and bartonella henselae infection]. 200011003916
cloning, sequencing, and expression of three bartonella henselae genes homologous to the agrobacterium tumefaciens virb region.a 17-kda, immunodominant antigen of bartonella henselae houston-1 has previously been cloned, sequenced, and characterized. this clone (h13) contains the 17-kda antigen gene plus a partial open reading frame, designated orf1, which is 459 nucleotides long and is directly upstream of the 17-kda gene. comparison of the deduced partial amino acid sequence of orf1 with that of other known genes in genbank revealed significant identity with several other bacterial virulence genes, including virb4 of ...200010749166
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