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comparative study of the nature and biological activities of bacterial enterotoxins.it is apparent that there are considerable similarities between many of the enterotoxins produced by enteric pathogens. although the effect of most of these toxins is restricted to the intestine in vivo, many cells are also sensitive to intoxication in vitro. the resultant in-vitro biochemical changes may have no pathological significance but serve to underline the central role of cyclic nucleotides in cellular fluid regulation. the biological activity of these enterotoxins is the result of inte ...19846327986
aeromonas hydrophila and plesiomonas shigelloides as causes of intestinal infections.infections due to aeromonas hydrophila and plesiomonas shigelloides in immuno-compromised hosts have been well documented, but the role of these organisms in causing gastrointestinal disease in healthy persons is controversial. despite difficulties in characterizing the exotoxins produced by aeromonas species, there is accumulating evidence that these bacteria are capable of causing usually mild, self-limited diarrheal disease in previously healthy adults. some pediatric patients may have protra ...19846390638
aeromonas: biology of the organism and diseases in children. 19846374630
[pathogen distribution in waste water sprinkler irrigation].the spray irrigation with pretreated wastewater was investigated on the sewage farm of the braunschweig sewage utilization association. the emission of airborne bacteria was measured by means of andersen sampler (as), reuter centrifugal sampler (rcs), and sedimentation plates (sp). there was a good correlation of results obtained by parallel measurings of as and rcs. the rcs was more effective by the factor 11.5 than the as sampling airborne microorganisms. however, the as gaves the distribution ...19846377753
isolation of aeromonas hydrophila from a metropolitan water supply: seasonal correlation with clinical isolates.the occurrence of aeromonas spp. in the metropolitan water supply of perth, western australia, australia, was monitored at several sampling points during a period of 1 year. water within the distribution system conformed to international standards for drinking water but contained aeromonas spp. in numbers comparable to those in raw surface water, although this water was free of escherichia coli. coliforms and e. coli were found in raw surface waters, and aeromonas spp. were found in raw water fr ...19846385848
[spoilage microorganisms encountered in ultra-high temperature processed milk].40 strains of aerobic or facultative anaerobic microorganisms were isolated from a total of 37 spoiled ultra high temperature processed milk. 13 of them were identified as the genus bacillus. they were 6 b. cereus, 5 b. licheniformis, 1 b. brevis and 1 b. pumilus. the other 27 strains were nonsporeforming microorganisms, which included 5 yeasts, 2 pseudomonas sp., 3 streptococcus sp., 12 lactobacillus sp., 1 shigella sp., 1 aeromonas sp. and 3 micrococcus sp. results indicate that the spoilage o ...19846468029
[distribution of enterobacteria in soil following irrigation with waste water].the filtration of wastewater after irrigation by sandy soil in the area of the braunschweig sewage utilization association was investigated. for that purpose, the concentrations of gram-negative aerobe rods were determined in soil samples of 15 cm, 30 cm, 45 cm, 60 cm, 75 cm, 90 cm, 105 cm, 120 cm, 135 cm, and 150 cm depth immediately after an irrigation of about 50 mm wastewater during the vegetation (3 series), the vegetationless period (6 series), after one week (3 series) and after two weeks ...19846475379
[sensitivity of diarrhea organisms to nifuroxazide]. 19846386203
aeromonas. 19846389407
structural and immunochemical homogeneity of aeromonas salmonicida lipopolysaccharide.sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the lipopolysaccharides of typical and atypical strains of the fish pathogen aeromonas salmonicida. 32p intrinsically radiolabeled lipopolysaccharide in sarcosinate-extracted outer membrane preparations, lipopolysaccharide stained by silver in proteinase k-digested outer membrane preparations and whole cell lysates, as well as purified lipopolysaccharide, displayed o-polysaccharide chains which were unusually homogeneo ...19846370955
purification and some properties of aeromonas hydrophila hemolysin.a hemolysin produced by a strain of aeromonas hydrophila isolated from a patient with diarrhea was purified by acid precipitation and quarternary aminoethyl-sephadex chromatography. the molecular weight of the hemolysin was estimated at 50,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and at 48,000 by sephadex g-100 gel filtration. in polyacrylamide gel electrophoresis at ph 4.0 and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the hemolysin migrated as a single b ...19846480102
[partial purification of the extracellular proteolytic system of aeromonas hydrophila lp50: comparative chromatographic and electrophoretic study].a complex extracellular proteolytic system was produced by aeromonas hydrophila lp50 on glucose- polypeptone medium. partial purification of this system was accomplished by ammonium sulphate precipitation, acetone precipitation, gel filtration on sephacryl- s200 and chromatography on deae-sephacel. every stage was controlled by electrophoresis. this proteolytic system was constituted of three aminopeptidase and two endopeptidase components.19846372579
aspirin in acute gastroenteritis: a clinical and microbiological study.soluble aspirin given by mouth in divided dosage decreased intestinal fluid loss in infants and young children with acute gastroenteritis. the treated group had significantly less diarrhea, which ceased earlier and needed less intravenous therapy, than a randomly selected control group given an indistinguishable placebo. this effect of aspirin occurred with diarrhea caused by salmonella, aeromonas, escherichia coli producing heat-labile toxin, and rotavirus, but not with diarrhea associated with ...19846389815
[aeromonas hydrophila in ivory coast epidemiologic and ecologic aspects and its enteropathogenic role].a research based on the analysis of 479 coprocultures , allow the isolation of 22 strains of aeromonas hydrophila. the epidemiological aspects, the contamination processes through water, food... and the enteropathogenic role of these aeromonas are discussed. these bacteria seen responsible of digestive disturbances from simple gastric discomfort (nausea, light abdominal pain...) to the actual choleriform syndrome. the isolation of 7 strains from the environment (water from lagune , wells; lagune ...19846327100
[an aeromonas hydrophila choleriform syndrome. apropos of a case from the ivory coast].an observation of a case of choleriform syndrome of the aeromonas hydrophila subspecies anaerogenes biotype ii is reported. in a country dealing with a severe comma bacillus under endemo -sporadic from, the case report here could be wrongly diagnosed as cholera.19846327101
biosynthesis of bacterial glycogen: characterization of adenosine diphosphate glucose synthetases from enterobacter hafniae and aeromonas hydrophila.enterobacter hafniae and aeromonas hydrophila adpglucose synthetases were purified approximately 39- and 61-fold, respectively, over the crude extract. both enzymes were heat stable at 60 degrees c in the presence of inorganic phosphate. the molecular weights of both enzymes were approximately 200,000 which are similar to other enteric adpglucose synthetases studied. based on kinetic results obtained from the partially purified enzymes, the e. hafniae enzyme is activated twofold by phospho-enolp ...19846331331
prophylactic doxycycline for travelers' diarrhea in thailand. further supportive evidence of aeromonas hydrophila as an enteric pathogen.a randomized double-blind study to determine the efficacy of a three-week course of doxycycline (100 mg daily) in preventing travelers' diarrhea was performed in 1980 among 63 united states peace corps volunteers during their first five weeks in thailand, an area where doxycycline-resistant enterotoxigenic escherichia coli are known to be common. eight (24%) of 33 volunteers taking placebo and 3 (10%) of 30 taking doxycycline developed travelers' diarrhea for a calculated protection of 59%, but ...19846391157
[demonstration of a proteolytic activity on intracellular origin in aeromonas hydrophila lp 50].aeromonas hydrophila lp 50, isolated from packaged pasteurized milk, was grown in glucose-polypeptone medium at 30 degrees c. the proteolytic activity of a. hydrophila lp 50, optimum at the stationary phase of growth, is attributed to extracellular or membrane protease; no intracellular proteolytic activity was shown.19846391644
[evaluation of results in the identification of various aerobic gram-negative bacteria in selected bacteriological laboratories in poland]. 19846392790
the impact of terrestrial and estuarial factors on the density of environmental bacterial (vibrionaceae) and faecal coliforms in coastal water.a study of the densities of faecal coliforms, vibrio anguillarum, vibrio alginolyticus and aeromonas hydrophila in danish bathing areas was carried out over the period may-october, 1981 with special reference to specific terrestrial and estuarial determinants. significant correlations were found between v. anguillarum and v. alginolyticus (r = 0.57) and a. hydrophila and faecal coliforms (r = 0.43). the densities of the vibrios were highly influenced by temperature and distance from pollution so ...19846395571
evaluation of a two-hour method for screening pathogens from stool specimens.the 2-h stool-screening test (sst) (api system s.a., montalieu-vercieu, france) was used to screen 231 organisms yielding suspicious colonies on stool differential agars for potential pathogens. all 54 salmonellae yielded correct screens. of 14 shigellas, 9 keyed as possible shigella spp.-yersinia enterocolitica-rare salmonella spp. (sys), and 5 (all shigella sonnei) keyed as possible s. sonnei-y. enterocolitica-arizona spp. (sya). three arizona strains were identified as probable salmonella spp ...19846386849
[severe aeromonas hydrophila infections. apropos of 2 cases].the authors describe two personal cases of severe aeromonas hydrophila infection. they review the symptomatology and treatment of such cases and insist on the unusual features in their patients.19846322344
collaborative evaluation of the abbott avantage system for identification of frequently isolated nonfermentative or oxidase-positive gram-negative bacilli.the capability of the abbott avantage system to identify 10 species of commonly isolated glucose nonfermentative or oxidase-positive gram-negative bacilli in a 5-h test period was evaluated in a collaborative study. the avantage nonenteric data base uses 20 biochemical test reactions performed in an expanded abbott bacterial identification cartridge plus the results of a manual oxidase test. the species included in the avantage data base are acinetobacter anitratus, acinetobacter iwoffi, aeromon ...19846392323
isolation of enterotoxigenic, hemolytic, and antibiotic-resistant aeromonas hydrophila strains from infected fish in bangladesh.strains of aeromonas hydrophila isolated from skin infections of common freshwater fish in bangladesh were tested for enterotoxin production, hemolysin production, and any correlation between these two activities. we also tested the resistance patterns of a. hydrophila to different drugs, especially in relation to ampicillin. the a. hydrophila strains produced an enterotoxin that was related to their beta-hemolytic activities. production of beta-hemolysin may thus be an indicator of enterotoxici ...19846508295
plasmid coding for transferable drug resistance in bacteria isolated from cultured rainbow trout.the occurrence of drug resistance and plasmid-mediated transferability was investigated in 170 strains belonging to eight bacterial groups isolated from cultured rainbow trout. it was found that 87.6% of the strains were resistant to at least one drug, with the highest percentages of resistance being detected for ampicillin (54.7%), sulfadiazine (46.5%), nitrofurantoin (38.2%), and chloramphenicol (37.0%). six enterobacteria, two vibrio, and one aeromonas isolate transferred resistance factors t ...19846508296
aeromonas septicemia with shock in children with thalassemias. 19846512449
value of blood agar for primary plating and clinical implication of simultaneous isolation of aeromonas hydrophila and aeromonas caviae from a patient with gastroenteritis.the simultaneous recovery of aeromonas hydrophila and aeromonas caviae from the stool of a 49-year-old woman with watery diarrhea was facilitated through the use of a blood agar medium which detected the hemolytic capability of a. hydrophila. in vitro phenotypic tests support the conclusion that only the a. hydrophila isolate was clinically significant.19846520231
surface layer virulence a-proteins from aeromonas salmonicida strains.superficial surface layer proteins (a-proteins) were present on diverse isolates of aeromonas salmonicida which differed both physiologically and in pathogenesis. three of these proteins were purified directly from the surface of whole cells or from outer membrane preparations. these a-proteins were unusually hydrophobic (45-47%) and of similar but not identical molecular mass (49, 50, and 51 kdaltons). they were nearly identical in amino acid composition and were highly conserved, but not ident ...19846525563
aeromonas hydrophilia tenosynovitis in an immunocompromised host. 19846536592
o-serogrouping scheme for mesophilic aeromonas strains.the o-antigens of 307 strains of mesophilic aeromonas including 227 a. hydrophila and 80 a. caviae were studied and 44 o-serogroups defined among them. the presence of heat-labile masked antigen, which inhibits o-agglutination, was observed in some strains. as all the o-antisera prepared with these mesophilic aeromonas strains contained some r-antibody, all diagnostic o-antisera must be absorbed with r-organisms before use. some of the o-antigens were found to be identical or closely related to ...19846536785
[aeromonas hydrophila: epidemiological study in the area around rome]. 19846536924
furunculosis in rainbow trout (salmo gairdneri) raised in sea water.ulcerative skin lesions were encountered in rainbow trout raised in sea water by a commercial concern in the western cape, south africa. grossly, the lesions resembled furunculosis but, histopathologically, they differed from typical furunculosis in that bacterial colonies were rarely found in the organs, and also the kidneys and spleens were minimally involved. the causative organism was identified as an achromogenic aeromonas salmonicida that shared characteristics with all 3 subspecies, salmo ...19846493730
aeromonas toxins. 19846494019
aeromonas infection in an infant with aplastic anemia. 19846494020
cloning of enterotoxin gene from aeromonas hydrophila provides conclusive evidence of production of a cytotonic enterotoxin.culture filtrates of two aeromonas hydrophila strains which were isolated from patients with diarrhea and assumed to be causative agents of the infections were shown to contain enterotoxic, cytotoxic, and hemolytic activities. modest heat treatment of the filtrates inactivated the cytotoxic and cytolytic activities, but not the enterotoxic activity. the construction of cosmid gene banks in escherichia coli of dna from both a. hydrophila strains demonstrated that the determinants of the three act ...19846500697
nucleotide base sequence of vibrionaceae 5 s rrna.nucleotide base sequences of 5 s rrnas isolated from vibrio vulnificus, vibrio anguillarum, and aeromonas hydrophila were determined. comparisons among these and sequences of 5 s rrnas from other species of vibrionaceae provide information useful in the evaluation of the evolution of bacterial species.19846479333
resistance of aeromonas hydrophila to beta-lactam antibiotics.the activity of some beta-lactam antibiotics upon 20 strains of aeromonas hydrophila and some properties of their beta-lactamases have been studied. high degree of resistance to benzylpenicillin and lower resistance to ampicillin and cephaloridine was observed. clavulanic acid showed the highest activity. when clavulanic acid was assayed at subinhibitory concentrations in association with ampicillin. mics of the latter decreased to two- to eight-fold. all strains produced inducible beta-lactamas ...19846335147
isolation of aeromonas spp. from an unchlorinated domestic water supply.the recovery of aeromonas spp. from the unchlorinated water supply for a western australian city of 21,000 people was monitored at several sampling points during a period of 1 year. membrane filtration techniques were used to count colonies of aeromonas spp., coliforms, and escherichia coli in water sampled before entry to service reservoirs, during storage in service reservoirs, and in distribution systems. aeromonas spp. were identified by subculture on blood agar with ampicillin, oxidase test ...19846486783
the occurrence of aeromonas hydrophila in avian diagnostic submissions.twenty isolations of aeromonas hydrophila were made from 15 species of free-living, commercial, and companion birds submitted for routine diagnostic postmortem examination. seventy percent of the isolations were made from november through march during the 25-month survey period.19846487200
structure of the lipopolysaccharide core isolated from a human strain of aeromonas hydrophila.lipopolysaccharide was isolated from the cell-walls of a human strain of aeromonas hydrophila by the aqueous phenol method in 0.58% yield (based on dry weight of bacteria). the lipopolysaccharide consisted of sr-polysaccharide, core-oligosaccharide and lipid a; there was no o-specific polysaccharide. the core had the composition d-galactose, d-glucose, d-glycero-d-manno-heptose, l-glycero-d-manno-heptose and d-glucosamine in a molar ratio of 1:1:2:4:1. glucosamine was linked to an l-glycero-d-ma ...19846489347
action of a microbial glycerophospholipid:cholesterol acyltransferase on plasma from normal and lcat-deficient subjects.the action of a bacterial acyltransferase similar in overall reaction mechanism to the plasma enzyme lecithin:cholesterol acyltransferase (lcat) has been studied using normal plasma and lipoproteins and plasma from lcat-deficient patients. the microbial enzyme (gcat) catalyzed acyl transfer using phosphatidylcholine and cholesterol in all of the lipoprotein fractions, presumably because it has no apolipoprotein cofactor. in addition, the enzyme was capable of hydrolyzing cholesteryl ester in lip ...19846491538
aeromonas hydrophila: a treatable cause of diarrhea. 19846607454
purification and properties of amino acid racemase from aeromonas punctata subsp. caviae.an amino acid racemase, which occurs in the cytoplasmic fraction of aeromonas punctata subsp. caviae, has been purified to homogeneity by the criteria of electrophoresis and ultracentrifugation. the enzyme has a molecular weight of about 80,000 and consists of two subunits identical in molecular weight (about 40,000). the enzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme, and exhibits absorption maxima at 280 nm and 420 nm. the holoenzyme is resolved by dialysis against hydroxyla ...19846427786
[aeromonas hydrophila septicemia: 3 cases, 1 with mediastinitis].three cases of aeromonas hydrophila septicaemia are reported. the first case concerned a 51 years old patient suffering from drowning who had previously been in good health and who died after a few days of refractory hypoxaemia. the second case was an 8 years old child with trisomy 21 operated for a congenital cardiac defect. the portal of entry was a catheter and the septicaemia was complicated by a. hydrophila mediastinitis, a complication which has not been described previously. the third cas ...19846476665
vaccination of fish: a practical view.commercial vaccines giving protection against three important bacterial fish diseases (enteric redmouth, furunculosis and vibriosis) became available in the united kingdom in 1983. the degree of protection obtained and the cost effectiveness vary according to the method of presentation (injection, dipping or spraying and oral dosing). these methods and the optimum time for vaccination are described.19846431685
aeromonas spp in travellers' diarrhoea. 19846434027
aeromonas spp in diarrhoea. 19846437556
difference in the extracellular products of two strains of aeromonas hydrophila virulent and weakly virulent for fish.in this study we first compared the toxigenic profile of aeromonas hydrophila strains virulent and weakly virulent for rainbow trout. the separation of the toxic factor for fish is also described. both strains produced hemolytic, enterotoxic, and dermonecrotic activities; the weakly virulent strain produced 20 times more hemolysin. only the cell-free supernatant of the virulent strain produced a toxic factor for fish. after passage on sephacryl s-200, the toxic factor for fish was separated from ...19846478313
comparative in vitro activities of apalcillin and piperacillin against gram-negative bacilli.the susceptibilities of 317 gram-negative bacilli to apalcillin and piperacillin were determined by standardized microdilution and disk diffusion tests. the respective percentages of strains susceptible to less than or equal to 64 micrograms of apalcillin and piperacillin per ml were as follows: members of the family enterobacteriaceae, 90 and 88%; randomly selected pseudomonas aeruginosa, 100 and 100%; multi-drug-resistant p. aeruginosa, 100 and 75%; and other nonfermenters and aeromonas hydrop ...19846440476
an unusual source of hospital wound infection. 19846440623
[infections caused by aeromonas hydrophila]. 19846700763
observations of bacterial diseases of captive snakes in finland.eighteen captive, nonpoisonous snakes which suffered from bacterial infections were studied. the most usual bacteria found were gram-negative rods; especially pseudomonas aeruginosa, aeromonas hydrophila and proteus spp. the most common disorders caused by them were stomatitis, septicaemia, bronchopneumonia and abscesses. the sensitivities of ten isolated bacteria to antimicrobial drugs were tested. neomycin and gentamicin seemed to be effective, especially against p. aeruginosa.19846728671
aeromonas hydrophila isolated from polyarthritis in a calf. 19846732673
thromboxane interaction with cardiopulmonary dysfunction in graded bacterial sepsis.the relationship between plasma levels of thromboxane a2, radioimmunoassayed as thromboxane b2 (txb), and cardiopulmonary dysfunction in graded bacterial sepsis was investigated. five adult female pigs under anesthesia were intubated and allowed to breathe room air spontaneously. femoral arterial, venous, and pulmonary artery catheters were inserted. after a 60-minute control period aeromonas hydrophila (1.0 x 10(9)/ml) was infused intravenously at 0.2 ml/kg/hr, gradually increasing to 4.0 ml/kg ...19846481830
brief report: bacteriological sampling of telephones and other hospital staff hand-contact objects.in an acute-care general hospital, 114 telephones, intercoms, dictaphones, and bedpan flusher handles were sampled in patient-care areas for type of bacterial contamination. nine of these (7%) demonstrated potentially pathogenic bacteria including klebsiella, enterobacter, pseudomonas and aeromonas. inanimate, environmental, staff hand-contact objects were only lightly contaminated, did not represent a significant reservoir of gram-negative organisms, and therefore, would be unlikely to be a veh ...19846568216
phylogenetic relationships among vibrio anguillarum plasmids.results of restriction endonuclease analysis and southern blot hybridization suggest that the r-plasmids from vibrio anguillarum strains isolated in japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. molecular cloning experiments allowed identification of specific restriction endonuclease fragments carrying the genes for either cmr or tcr as the common sequences between some of these groups of r-plasmids. the cmr region fr ...19846528000
aeromonas hydrophila colitis in a child.a 5-year-old girl presented with chronic bloody diarrhea. evaluation including sigmoidoscopy, rectal biopsy, and barium enema was consistent with the diagnosis of ulcerative colitis. culture of the stool grew aeromonas hydrophila. a. hydrophila colitis may be more common than presently realized.19846502383
media for isolation of aeromonas spp. from faeces.five solid media were evaluated for isolation of aeromonas spp. from faeces: desoxycholate citrate agar (dca), macconkey's agar (mac), xylose-desoxycholate-citrate agar (xdca), rogol's medium (rog), which contained ampicillin 20 mg/l and p-nitrophenyl-glycerine 25 mg/l as inhibitors, and blood agar (ba) with ampicillin 10 mg/l. false negative oxidase tests limited the usefulness of dca and mac and, although the use of xdca avoided the problem of fermentation of lactose, some aeromonas spp. faile ...19846502678
production of cholera-like enterotoxin by aeromonas hydrophila.a total of 249 strains of mesophilic aeromonas including 179 a. hydrophila and 70 a. caviae were tested for production of cholera-like enterotoxin by reversed passive latex agglutination (rpla) assay. a cholera-like enterotoxin neutralized with cholera antitoxin was demonstrated in the culture filtrates from eight (4.5%) of the 179 a. hydrophila strains, while none of a. caviae strains revealed the enterotoxin production in the test. production of the cholera-like enterotoxin in the eight strain ...19846503026
enterotoxin production in relation to taxonomic grouping and source of isolation of aeromonas species.a total of 19 of 20 (95%) strains of aeromonas hydrophila biovar hydrophila and 16 of 17 (94%) strains of aeromonas sobria isolated from a variety of clinical and environmental sources were found to be enterotoxin positive. only 2 of 18 (11%) a. hydrophila biovar anaerogenes and 2 of 13 (15%) unidentified aeromonas strains from a similar variety of sources produced enterotoxin. no association was apparent between the source of isolation, in particular diarrheal stools, and enterotoxigenicity; 41 ...19846699147
hemagglutination patterns of aeromonas spp. in relation to biotype and source.aeromonas spp. show patterns of hemagglutination with human group o cells in the presence of fucose, galactose, and mannose. these patterns are related to biotype as well as to the source of isolates. there was good correlation between hemagglutination pattern and the presence of diarrhea among strains isolated in western australia, which was the only source with adequate data for classification of children with an without diarrhea. most of the environmental and other nonfecal isolates produced ...19846690466
biotyping of aeromonas isolates as a correlate to delineating a species-associated disease spectrum.a group of 147 aeromonas isolates from diverse clinical and environmental sources was subjected to the biotyping scheme of popoff and veron. of the 147 isolates biotyped, 137 (93%) could be identified, with aeromonas hydrophila predominating (48%) and equal percentages (25 to 27%) of the other two species (aeromonas sobria and aeromonas caviae). a number of additional biochemical properties were found to be significantly associated with one or more of these three species. these included lysine d ...19846690467
bacterial interference with coliform colony sheen production on membrane filters.the membrane filter (mf) method for detection and enumeration of coliform bacteria in drinking water requires that the coliforms both grow and produce a green metallic sheen when the filter is incubated on modified endo medium at 35 degrees c for 22 h. large numbers of noncoliform bacteria, which are enumerated by the standard plate count (spc) technique, can interfere with the detection of coliforms on mf. this paper presents quantitative evidence from laboratory experiments on the interference ...19846696423
aeromonas hydrophila gastroenteritis in tuscany children. 19846739332
biotyping and virulence factors in clinical and environmental isolates of aeromonas species.biochemical characteristics and virulence factors were compared in 147 aeromonas spp. isolated from patients with diarrhea and in 94 strains isolated from metropolitan water supplies in the same area during the same period. fermentation of arabinose occurred with 58.5% of the environmental strains and 15% of the clinical isolates; 39.4% of the strains from water and 6.8% of the fecal isolates fermented salicin. the frequency of esculin hydrolysis was the same in both groups. ninety-one percent o ...19846742829
severe cellulitis due to aeromonas hydrophilia following immersion injury. 19846745473
aeromonas septicemia in infants and children.our experience at the ramathibodi hospital with 20 infants and children who had aeromonas septicemia is reviewed. their ages were from 1 day to 14 years. eighteen patients had underlying diseases: leukemia, 5; aplastic anemia, 4; cirrhosis, 2; thalassemia/hemoglobinopathy, 3; renal failure, 1; ileal perforation, 1; marasmus, 1; and cavernous hemangioma with thrombocytopenia, 1. blood cultures yielded aeromonas hydrophila in all patients, and four patients had polymicrobial bacteremia. fifteen ep ...19846728702
aeromonas hydrophila in livestock: incidence, biochemical characteristics and antibiotic susceptibility.faecal samples from 110 horses, 115 pigs, 111 sheep and 123 cows were examined for the presence of aeromonas hydrophila, which was also sought in the available drinking water. the overall faecal rate was 11.8%, but significantly more bovine than other samples were found to be positive. there was significant association between the isolation of a. hydrophila from all animal faeces and its presence in drinking water, but this was not found when individual animal groups were analysed separately. an ...19846736644
septicemia due to aeromonas hydrophila. 19846736824
phospholipids and lipopolysaccharide of aeromonas hydrophila.the phospholipids and lipopolysaccharide of aeromonas hydrophila were characterized. phosphatidylethanolamine and phosphatidylglycerol were the major phospholipid components. the outer membrane contained more phosphatidylethanolamine and less phosphatidylglycerol than the inner membrane, and the phospholipids of the outer membrane contained a higher proportion of saturated fatty acids. only four fatty acids (c14:0, c16:0, c16:1, and c18:1) were found in the phospholipids. the lipopolysaccharide ...19853968030
comparative in vitro activities of selected antimicrobial agents against aeromonas species and plesiomonas shigelloides.the in vitro antimicrobial susceptibilities of fecal isolates of aeromonas caviae, aeromonas hydrophila, aeromonas sobria, and plesiomonas shigelloides were tested by agar dilution. this in vitro study suggested that ciprofloxacin, enoxacin, norfloxacin, tetracycline, and trimethoprim-sulfamethoxazole might be effective oral agents for treatment of diarrhea involving aeromonas spp. or p. shigelloides.19854004196
immunity to aeromonas salmonicida in coho salmon (oncorhynchus kisutch) induced by modified freund's complete adjuvant: its non-specific nature and the probable role of macrophages in the phenomenon.juvenile coho salmon (oncorhynchus kisutch), vaccinated with one intraperitoneal injection of formalin-killed virulent aeromonas salmonicida cells suspended in saline, showed increased protection against approximately one ld60 of homologous challenge administered at 30 days post-vaccination. under similar conditions, coho vaccinated with a modified complete freund's adjuvant (mfca) alone were also equally protected. when measured against a more severe a. salmonicida challenge of approximately on ...19854043481
the purification and some properties of h-lysin from aeromonas salmonicida.h-lysin from aeromonas salmonicida has been purified 1770-fold by freeze fractionation, ammonium sulphate precipitation, ion exchange chromatography and gel filtration chromatography. the purified material was predominantly h-lysin, devoid of detectable t-lysin, caseinase or gelatinase activity, although glycerophospholipid: cholesterol acyltransferase (gcat) activity was present. the results suggested that h-lysin and gcat activities were due to different extracellular products. studies of the ...19854045422
the antagonism of tetracycline and ferric iron in vivo.to test the hypothesis that the in-vivo antibiotic action of tetracycline might be affected by ferric iron and the enhancement of infection by ferric iron by tetracycline, the actions of intraperitoneal antibiotic and local ferric ammonium citrate, given separately and together, were measured in the dorsal skin of guinea-pigs bearing lesions due to staphylococci, streptococci, a proteus sp., an erysipelothrix sp., clostridium perfringens, pseudomonas aeruginosa, aeromonas hydrophila and klebsiel ...19854020849
[metabolic traits of plesiomonas shigelloides strains isolated from children with acute enteritis]. 19854048753
unfamiliar bacteria isolated from cats. 19854049692
demonstration of cholera toxin-related factor in cultures of aeromonas species by enzyme-linked immunosorbent assay.the production of toxins by aeromonas species was examined by the suckling mouse test, the hemolysin test, and the enzyme-linked immunosorbent assay with anticholera enterotoxin. a factor that was immunologically related to cholera enterotoxin was produced by 5 of 14 strains of aeromonas hydrophila and 4 of 15 strains of aeromonas sobria. analysis by these assays and by a test for heat stability suggested that the factor differed from hemolysin and from toxin that was active in the suckling mous ...19854044041
lack of correlation between known virulence properties of aeromonas hydrophila and enteropathogenicity for humans.five strains of aeromonas hydrophila were selected for use in volunteer challenge trials. all five strains produced cytotoxin, hemolysin enterotoxin, lysine decarboxylase, acetylmethylcarbinol, and dnase. two strains hydrolyzed esculin. all strains produced purulent hemorrhagic fluid accumulation in rabbit ileal loops, but failed to induce keratoconjunctivitis in guinea pigs. none of the strains produced mannose-resistant hemagglutinins. in challenge studies, diarrhea was demonstrated in only 2 ...19854044042
invasiveness of aeromonas spp. in relation to biotype, virulence factors, and clinical features.of 69 fecal isolates of aeromonas spp., 18 had the ability to invade hep-2 cells. invasiveness correlated with biotype; of the 18 invasive strains, 16 were a. sobria and 2 were a. hydrophila. no invasive strains were found among the a. caviae. of the 18 invasive strains, 13 were enterotoxigenic. of the enterotoxigenic and invasive strains, 12 were a. sobria, but enterotoxicity was also more common among noninvasive strains of a. sobria. fucose-resistant hemagglutination was also more common in a ...19854019741
invasion of hep-2 cells by fecal isolates of aeromonas hydrophila.human diarrheal isolates of aeromonas hydrophila were found to be invasive in hep-2 cell monolayers. microscopic examination of two isolates from patients with symptoms of dysentery revealed that infected hep-2 cells contained up to 50 bacteria localized within areas of the cytoplasm. a quantitative invasion assay was developed, using viable counts of total intracellular bacteria. five fecal isolates of a. hydrophila were classified as invasive, with an average of 0.1 up to 2 bacteria per hep-2 ...19853972447
protein export by a gram-negative bacterium: production of aerolysin by aeromonas hydrophila.the synthesis and export of aerolysin, an extracellular protein toxin released by the gram-negative bacterium aeromonas hydrophila, was studied by pulse-labeling with [35s]methionine. the toxin was synthesized as a higher-molecular-weight precursor. this was processed cotranslationally, resulting in the appearance within the cell of the mature protein, which was then exported to the supernatant. precursor aerolysin accumulated in cells incubated in the presence of carbonyl cyanide m-chlorophenyl ...19853972770
identification of hydroxy fatty acids in aeromonas hydrophila, aeromonas sobria, and aeromonas caviae.gas-liquid chromatographic analysis of fatty acid methyl esters obtained from clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae revealed the presence of two hydroxy fatty acid species, 3-oh 12:0 and 3-oh 14:0.19853934208
clinical predictors of acute bacterial diarrhea in young children.this prospective study assessed the value of presenting history, physical examination, and screening laboratory tests in predicting whether diarrhea in a young child is associated with a stool culture positive for a bacterial pathogen. acutely ill children less than 4 years old were studied in a hospital outpatient setting. two hundred patients were seen in a 9 1/2-month period, which encompassed the seasons of summer, fall, and winter. one hundred ninety-five patients had cultures completed and ...19854047797
in vitro activities of the quinolone antimicrobial agents a-56619 and a-56620.the in vitro activities of two new quinolone antimicrobial agents, a-56619 and a-56620, were compared with those of norfloxacin, ciprofloxacin, and other antimicrobial agents. the activity of a-56620 was comparable to that of ciprofloxacin against escherichia coli, enterobacter cloacae, and aeromonas hydrophila (mics for 90% of the strains were less than or equal to 0.06 micrograms/ml); acinetobacter anitratus and staphylococcus aureus (mic for 90% of the strains was 0.5 micrograms/ml); and most ...19853935046
pathogenicity of aeromonas.the ability of 15 aeromonas sobria and 9 aeromonas hydrophila isolates to cause subcutaneous lesions was tested. an inoculum of 10(11) colony forming units/l was injected subcutaneously into mice. surviving animals developed a subcutaneous abscess and/or localised skin sloughing and loss of hair (alopecia). an abscess was induced by all nine a. hydrophila isolates and by three of the 15 a. sobria isolates. the induction of local epidermal sloughing and loss of hair followed challenge with either ...19853981022
[spontaneous aeromonas hydrophila peritonitis in the cirrhotic. apropos of 2 cases]. 19854001643
aeromonas hydrophila--an accepted enterotoxigenic "neoenteropathogen"? 19853989329
comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae.sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ...19854056003
cephalothin susceptibility as a potential marker for the aeromonas sobria group.fifty-four motile aeromonas strains, composing the three currently recognizable species, were tested for susceptibility to cephalothin by broth dilution and disk agar diffusion assays. cephalothin susceptibility was significantly associated with aeromonas sobria (p less than 0.001) and may be an additional phenotypic marker useful in the identification of this species.19854056009
bacteremia caused by aeromonas species in hospitalized cancer patients.bacteremia caused by aeromonas species occurred in 24 hospitalized patients in a cancer institute during a 13-year period. all but one of these patients had a malignancy (88% had leukemia), and most were receiving chemotherapy for cancer. there was a striking numerical predominance of male patients (82%). unlike some previously described patients with infections due to this organism, none of these 24 patients had recently been exposed to fresh or salt water or to fish. the source of the infectin ...19854023526
antibacterial activity of amifloxacin (win 49, 375), a new quinolone agent.the in vitro activity of amifloxacin, a quinolone antimicrobial agent was compared with those of ciprofloxacin, enoxacin, ofloxacin and norfloxacin against gram-positive and gram-negative bacteria. ninety percent of escherichia coli, klebsiella species, aeromonas, salmonella, shigella, citrobacter, enterobacter species, proteus mirabilis, serratia marcescens, and morganella morganii were inhibited by less than or equal to 0.5 microgram/ml. amifloxacin inhibited branhamella, haemophilus, and neis ...19854064609
aeromonas caviae: an enteric pathogen?of the aeromonas spp. isolated routinely in our laboratory from human feces, about two thirds represent aeromonas caviae. in contrast to aeromonas hydrophila and aeromonas sobria, this species has been considered to be of little enteropathogenic significance due to the absence of known virulence factors. the clinical data presented here suggest a clinical significance of a. caviae, at least in some cases. as yet nothing is known about the pathogenic mechanisms involved.19854066046
a transition-state-analog inhibitor influences zinc-binding by aeromonas aminopeptidase.the transition-state-analog inhibitor, 1-butaneboronic acid, markedly enhances the uptake of one g-atom of zn2+ ions from a metal ion buffer system by zn-depleted aeromonas aminopeptidase. in contrast, a substrate-analog inhibitor, n-valeramide, does not perturb the equilibrium between zn2+ ions and the enzyme in a metal ion buffer system. these results establish a role for metal ions in the binding of 1-butaneboronic acid to aeromonas amino-peptidase and strongly imply that a bound zn2+ ion int ...19854026862
salmonellae and pollution indicator bacteria in municipal and food processing effluents and the cornwallis river.a study was conducted to determine the incidence of fecal coliforms, fecal streptococci, aeromonas hydrophilia, and salmonella spp. in the waste discharges of seven sewage treatment plants, four fruit and vegetable canneries, a meat packing plant, a poultry processing plant, and a potato processing plant located along the cornwallis river in nova scotia, canada. surface water samples were also collected from 13 locations in the river to assess the impact of these waste discharges on the receivin ...19854027779
aeromonas-related diarrhea in adults.we have reviewed the incidence of aeromonas in patients with enteric disease at our hospital and found it to be the highest of any potential enteric pathogen. eighty adult patients with diarrhea had aeromonas isolated from feces, and in 73 aeromonas was the only potential bacterial or parasitic pathogen detected. the spectrum of illness in patients with aeromonas-related diarrhea ranged from acute, self-limited diarrhea to a chronic, indolent diarrheal illness. sixteen percent (13/80) of the pat ...19854074035
methods for the detection of haemagglutinins in aeromonas.when grown in specified conditions and tested by a rocked-tile method, 40 of 41 isolates of two species of aeromonas formed simultaneously at least two haemagglutinins among which were: (i) a mannose-sensitive haemagglutinins with strongest activity for guinea-pig or fowl red cells, formed by all of 31 isolates of a. hydrophila and 9 of 10 isolates of a. punctata ss. caviae; (ii) a haemagglutinin, sensitive to l-fucose or d-mannose, that reacted with human red cells and which was formed by all 4 ...19853981614
media for the isolation of aeromonas hydrophila.isolation rates of aeromonas hydrophila from stool samples of symptomatic and asymptomatic individuals were examined for several common enteric media. sheep blood agar with 10 micrograms of ampicillin per ml, preceded by overnight enrichment in alkaline peptone water, yielded 2.6 times the number of isolates as the other media examined and is recommended for the isolation of a. hydrophila from humans.19854056015
biochemical and immunological characterization of the cell surface of the fish pathogenic bacterium aeromonas salmonicida.the identification of lipopolysaccharide as periodic acid-schiff positive material, present in the membrane fraction of the fish pathogenic gram-negative bacterium aeromonas salmonicida, analyzed by sds-polyacrylamide gel electrophoresis, is shown. such analysis has revealed several periodic acid-schiff positive bands and many membrane proteins among which a pathogenicity-related mr 54000 protein as a constituent of an additional surface layer outside the outer membrane (evenberg et al., (1982) ...19853995026
comparison of the cathra repliscan ii, the automicrobic system gram-negative general susceptibility-plus card, and the micro-media system fox panel for dilution susceptibility testing of gram-negative bacilli.a comparative evaluation was done to test the accuracy of the cathra repliscan ii agar dilution system (diagnostic equipment, inc., st. paul, minn.), the automicrobic system with gram-negative general susceptibility-plus card (vitek systems, inc., hazelwood, mo.), and the micro-media fox panel micro broth dilution system (micro-media systems, inc., san jose, calif.) in determining mics of 12 antibiotics for 200 gram-negative bacilli. of the 200 strains tested, 12 isolates did not grow in one of ...19854008625
[amino acid composition of white poultry meat contaminated with psychrophilic microorganisms].studied was the effect of psychrophilic organisms of the acinetobacter, aeromonas, alcaligenes, and flavobacter genera on the amino acid composition of white poultry meat stored at 0 degrees to 4 degrees c in the course of 48 hours, and at 18 degrees c in the course of 6 to 12 months. the quantitative determination of individual amino acids was carried out by means of an automatic amino acid analyzer, hitachi type, after the method of speckman, stein, and moore. no changes were established in th ...19854035999
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