Publications

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evaluating the occurrence of escherichia albertii in chicken carcass rinses by pcr, vitek analysis, and sequencing of the rpob gene.escherichia albertii is a recently described species that has been associated with gastroenteritis in humans and with healthy and ill birds. most recently, it has been identified as the causative agent in a food-borne outbreak in japan. the distribution and clinical importance of e. albertii are not well studied because its importance is unclear. culture methods for clinical isolation frequently miss e. albertii or incorrectly identify it as shigella spp., escherichia coli, or hafnia alvei. this ...201525548040
the plasminogen activator content of the arterial wall in occlusive arterial diseases. 20154662619
pyrosequencing analysis of microbial community and food-borne bacteria on restaurant cutting boards collected in seri kembangan, malaysia, and their correlation with grades of food premises.this study adopts the pyrosequencing technique to identify bacteria present on 26 kitchen cutting boards collected from different grades of food premises around seri kembangan, a city in malaysia. pyrosequencing generated 452,401 of total reads of otus with an average of 1.4×10(7) bacterial cells/cm(2). proteobacteria, firmicutes and bacteroides were identified as the most abundant phyla in the samples. taxonomic richness was generally high with >1000 operational taxonomic units (otus) observed ...201525679309
chemical composition and antibacterial activity of lavandula coronopifolia essential oil against antibiotic-resistant bacteria.the aim of this study was to analyse the composition of the essential oil (eo) of lavandula coronopifolia from morocco and to evaluate its in vitro antibacterial activity against antibiotic-resistant bacteria isolated from clinical infections. the antimicrobial activity was assessed by a broth micro-well dilution method using multiresistant clinical isolates of 11 pathogenic bacteria: klebsiella pneumoniae subsp. pneumoniae, klebsiella ornithinolytica, escherichia coli, enterobacter cloacae, ent ...201525174508
application of bacteriophages to reduce biofilms formed by hydrogen sulfide producing bacteria on surfaces in a rendering plant.hydrogen sulfide producing bacteria (spb) in raw animal by-products are likely to grow and form biofilms in the rendering processing environments, resulting in the release of harmful hydrogen sulfide (h2s) gas. the objective of this study was to reduce spb biofilms formed on different surfaces typically found in rendering plants by applying a bacteriophage cocktail. using a 96-well microplate method, we determined that 3 spb strains of citrobacter freundii and hafnia alvei are strong biofilm for ...201526102989
prevalence and characterization of esbl- and ampc-producing enterobacteriaceae on retail vegetables.in total 1216 vegetables obtained from dutch stores during 2012 and 2013 were analysed to determine the prevalence of 3rd-generation cephalosporin (3gc) resistant bacteria on soil-grown fresh produce possibly consumed raw. vegetables grown conventionally and organically, from dutch as well as foreign origin were compared. included were the following vegetable types; blanched celery (n=192), bunched carrots (n=190), butterhead lettuce (n=137), chicory (n=96), endive (n=188), iceberg lettuce (n=19 ...201525828704
antibacterial susceptibility of enterobacteriaceae isolated from raw horsemeat isolated for human consumption (basashi).drug susceptibility testing was carried out using 14 antibiotics in order to identify trends in the antibiotic tolerance of 142 strains of enterobacteriaceae isolated from horsemeat commercially available for raw consumption (basashi). a comparison of the sensitivity to the 14 antibiotics using the 90% mic (minimum inhibitory concentration) values (mic90) showed the strongest tolerance to ampicillin (abpc) at a concentration of > 128 μg/ml, followed by that to fosfomycin (fom) at a concentration ...201525817809
purification, biochemical characterization, and genetic cloning of the phytase produced by burkholderia sp. strain a13.a phytase-producing bacterium, burkholderia sp. a13 (jcm 30421), was isolated from lake kasumigaura by enrichment cultivation using minimum medium containing phytic acid as the sole phosphorus source. the phytase production by strain a13 was induced by the presence of phytic acid and repressed by the addition of glucose. the purified enzyme had a molecular weight of 44 kda and a phytase activity of 174 μmol min(-1) mg(-1). the enzyme showed broad substrate specificity, but the highest activity w ...201525833676
microbiota and metabolome of un-started and started greek-type fermentation of bella di cerignola table olives.this study aimed to utilize an "omics" approach to evaluate the ability of selected lactobacilli and yeasts to improve the fermentation process of bella di cerignola table olives. four types of fermentations were performed at the pilot-plant scale: un-started fermented olives used as a control (ctrl); olives started with a commercial lactobacillus plantarum strain (s); commercial l. plantarum strain and autochthonous yeast wickeramomyces anomalus disspa73 (sy); and l. plantarum, w. anomalus diss ...201526338113
hafnia psychrotolerans sp. nov., isolated from lake water.a psychrotolerant, gram-stain-negative, motile, aerobic, peritrichous bacterium, strain djc1-1(t), was isolated from lake dajiaco, tibetan plateau, china. the strain was negative for citrate utilization, lipase activity and α-glucosidase, but positive for the voges-proskauer reaction and n-acetyl-β-glucosaminidase. 16s rrna gene sequence analysis indicated that hafnia paralvei atcc 29927(t), hafnia alvei atcc 13337(t), serratia grimesii dsm 30063(t) and serratia plymuthica dsm 4540(t) were the c ...201525563917
occurrence of glycine in the core oligosaccharides of hafnia alvei lipopolysaccharides--identification of disubstituted glycoform.endotoxins (lipopolysaccharides, lps) are the main surface antigens and virulence factors of gram-negative bacteria involved for example in the development of nosocomial infections and sepsis. they consist of three main regions: o-specific polysaccharide, core oligosaccharide, and lipid a. bacteria modify lps structure to escape the immune defence, but also to adapt to environmental conditions. lps's structures are highly diversified in the o-specific polysaccharide region to evade bactericidal ...201525541016
a methodological approach to screen diverse cheese-related bacteria for their ability to produce aroma compounds.microorganisms play an important role in the development of cheese flavor. the aim of this study was to develop an approach to facilitate screening of various cheese-related bacteria for their ability to produce aroma compounds. we combined i) curd-based slurry medium incubated under conditions mimicking cheese manufacturing and ripening, ii) powerful method of extraction of volatiles, headspace trap, coupled to gas chromatography-mass spectrometry (hs-trap-gc-ms), and iii) metabolomics-based me ...201525475278
ficolin-3 activity towards the opportunistic pathogen, hafnia alvei.ficolin-3 (also called h-ficolin or hakata antigen) is a complement-activating pattern recognition molecule, possessing a fibrinogen-like domain involved in carbohydrate binding. amongst human ficolins, ficolin-3 has the highest concentration in serum and is the most potent lectin pathway activator in vitro. evidence for its physiological function is sparse, although its deficiency has been suggested to increase susceptibility to infections. the specificity of ficolin-3 is poorly characterized a ...201525178935
[results of the surgical treatment of cancers of the transverse colon. apropos of 43 cases]. 20154652093
acedapsone in leprosy treatment: trial in 68 active cases in micronesia. 20154672994
[effect of broncasma berna on bronchial asthma. a double blind study]. 20154572552
cell response to surface stimulation. 20154508399
hafnia alvei urosepsis in a kidney transplant patient.hafnia alvei, a gram-negative facultative anaerobic, rod-shaped bacterium, is a rare cause of infection in humans. we report on a renal transplant patient who developed h. alvei pyelonephritis and urosepsis. the source of infection remains enigmatic but is most likely the intestinal tract. appropriate antibiotic therapy with cefepime followed by oral ciprofloxacin brought about rapid resolution of symptoms and complete recovery. h. alvei may cause severe infection in transplant patients without ...201525960912
draft genome sequence of hafnia paralvei strain gta-haf03.hafnia paralvei is a gram-negative member of the enterobacteriaceae family, closely related to the opportunistic pathogen hafnia alvei. we report here the first draft genome sequence of h. paralvei, from the beef trim isolate gta-haf03, consisting of a 5.0-mbp assembly encoding 4,382 proteins and 90 predicted rnas.201525700416
antibiotic resistance of gram-negative benthic bacteria isolated from the sediments of kardzhali dam (bulgaria).the aim of the present study was to carry out a preliminary assessment for the occurrence of bacterial strains resistant to frequently used antibiotics in the sediments beneath the sturgeon cage farm in kardzhali dam (bulgaria). samples were taken from the top 2 cm of sediments under a fish farm and from a control station in the aquatory of the reservoir in the period july-october 2011. surveillance of bacterial susceptibility to 16 antimicrobial agents was performed for 160 gram-negative strain ...201526019641
[role of change agent in planned change]. 20154531520
[individuation and communication in various neurosis structures]. 20154692550
[primary surgical treatment of punctate wounds in open fractures of the crural bones]. 20154610479
[2 cases of upper caval vein anomaly (diagnostic difficulties in mediastinal tumors)]. 20154774754
mental health: systems and non-systems. 20154357958
[biological activity of serotonin in the blood of patients with myocardial infarct]. 20154635547
[surgical treatment of complications associated with myocardial infarct: aneurysm of the left ventricle with interventricular communication and mitral insufficiency]. 20154681372
[surgical treatment of complications associated with myocardial infarct: aneurysm of the left ventricle with interventricular communication and mitral insufficiency]. 20154681372
some ethnographic insights into the history of dentistry: contemporary barber-toothdrawers. 20154505225
the adsorption of chloropicrin by carbon filters: characterisation of the breakthrough curves. 20154472344
[bone changes in diabetics]. 20154453288
[musculus rectus labii and its significance for the lip mobility of chimpanzees (pan troglodytes)]. 20154451282
[2 cases of granular cell myoblastoma of the breast]. 20154294687
the chain stitch knot. 20154607706
interactions between nutrition and heredity in coronary heart disease. 20154588183
normal values for blood constituents of the baboon (papio species). 20154315779
the histaminase activity of ceruloplasmin. 20154630290
[selectivity of action of propericiazine in behavior disorders]. 20154384082
mobile dental unit: construction and utilization. 20154534283
regeneration of hydra from reaggregated cells. 20154507522
criteria for a recommended standard occupational exposure to hot environments. i. recommendations for a standard for work in hot environments. 20154704229
criteria for a recommended standard occupational exposure to hot environments. i. recommendations for a standard for work in hot environments. 20154704229
further thoughts on thinking and language. 20154690362
agonal physiology and blood gas tensions. 20154644294
[acute leukemias: age, sex, occurence]. 20154531117
lichen planus. 20154524104
[photobadismographic demonstration of azapropazone]. 20154540946
modification of a standard bicycle ergometer for underwater use. 20154469099
[presence of insecticides in surface waters treated for drinking purposes]. 20154458695
clinical experience with bone marrow transplantation: failure and success. 20154398497
[progress in dental investment materials (author's transl)]. 20154527505
[history of curare research]. 20154877402
[papillary dysversions]. 20154433961
[generalized follicular and ungual mucinosis]. 20154642849
[tasks of the sport medicine physician]. 20154561355
[literature documentation--information system]. 20164805592
evaluation of the management of vehicular fatalities secondary to abdominal injury. 20165033485
[some controversial questions in urological terminology]. 20165052377
[experimental and clinical studies on the effect of trimanyl on lipid metabolism. i. effect of single dose administration on the general level of fats in blood]. 20164880072
[autoimmune hepatitis]. 20164865241
antenatal diagnosis of genetic disorders. 20164844728
spinal fluid cytomorphology. description of apparatus, technique, and findings. 20164862475
prolactin release during nursing and breast stimulation in postpartum and nonpostpartum subjects. 20164815172
[bile peritonitis after perforation of the gallbladder (author's transl)]. 20164809959
[the brow of the cotyle: reflection of the stresses in the hip joint]. 20164842218
[respiratory system--disease model by medical engineering approach]. 20165064636
[respiratory system--disease model by medical engineering approach]. 20165064636
vd--report it. 20165067414
alternative methods for the analysis of complex epidemiological data. 20165016159
intracarotid air embolism in the baboon: effects on cerebral blood flow and the electroencephalogram. 20164993586
twenty years ago. 20164869667
metabolism of the heart in health and disease. ii. 20164882561
nutritional and epidemiologic factors related to heart disease. 20164924054
[medical aspect of the treatment of extensive burns in children. (1st findings apropos of 8 cases)]. 20164775921
[systole phase and cardiac volume dynamics in dosed veloergometric loading in patients with ischemic heeart disease and its significance in detecting pre-clinical stage of cardiac insufficiency]. 20165043024
[300th anniversary of lucka pharmacy]. 20164931013
genetics and schizophrenia. 20164739404
nutrients in university food service meals. i. data determined by food inventory. 20165033381
[prospects for economical resection of the stomach with a gastroduodenal anastomosis in peptic ulcer]. 20164832331
whole-genome sequence of hafnia alvei humv-5920, a human isolate.a clinical isolate of hafnia alvei (strain humv-5920) was obtained from a urine sample from an adult patient. we report here its complete genome assembly using pacbio single-molecule real-time (smrt) sequencing, which resulted in a chromosome with 4.5 mb and a circular contig of 87 kb. about 4,146 protein-coding genes are predicted from this assembly.201627313299
genome sequence of hafnia alvei bta3_1, a bacterium with antimicrobial properties isolated from honey bee gut.hafnia alvei bta3_1, a strain with antibacterial properties, was isolated from honey bee gut and cultured under aerobic and anaerobic conditions. to explore the potential genetic bases of its antibacterial and possible pathogenic properties, the complete genome of this organism was sequenced and analyzed.201627284146
a new ligand-based method for purifying active human plasma-derived ficolin-3 complexes supports the phenomenon of crosstalk between pattern-recognition molecules and immunoglobulins.despite recombinant protein technology development, proteins isolated from natural sources remain important for structure and activity determination. ficolins represent a class of proteins that are difficult to isolate. to date, three methods for purifying ficolin-3 from plasma/serum have been proposed, defined by most critical step: (i) hydroxyapatite absorption chromatography (ii) n-acetylated human serum albumin affinity chromatography and (iii) anti-ficolin-3 monoclonal antibody-based affini ...201627232184
validity of an empirically derived phenomenological typology. 20165071939
clinical problems encountered in renal homotransplantation in children. 20164911478
[centenary of the birth of mining engineer i. m. puginov]. 20164901215
chemotherapy with hydroxyurea (nsc-32065) in renal cell carcinoma. 20164883783
control rate in diphenylhydantoin therapy. 20164717597
conjoint scaling of subjective number and weight. 20164705240
dectin-2 recognizes mannosylated o-antigens of human opportunistic pathogens and augments lipopolysaccharide activation of myeloid cells.lps consists of a relatively conserved region of lipid a and core oligosaccharide and a highly variable region of o-antigen polysaccharide. whereas lipid a is known to bind to the toll-like receptor 4 (tlr4)-myeloid differentiation factor 2 (md2) complex, the role of the o-antigen remains unclear. here we report a novel molecular interaction between dendritic cell-associated c-type lectin-2 (dectin-2) and mannosylated o-antigen found in a human opportunistic pathogen, hafnia alvei pcm 1223, whic ...201627358401
genetic diversity of o-antigens in hafnia alvei and the development of a suspension array for serotype detection.hafnia alvei is a facultative and rod-shaped gram-negative bacterium that belongs to the enterobacteriaceae family. although it has been more than 50 years since the genus was identified, very little is known about variations among hafnia species. diversity in o-antigens (o-polysaccharide, ops) is thought to be a major factor in bacterial adaptation to different hosts and situations and variability in the environment. antigenic variation is also an important factor in pathogenicity that has been ...201627171009
identification and growth dynamics of meat spoilage microorganisms in modified atmosphere packaged poultry meat by maldi-tof ms.modified atmosphere packaging (map) is widely used in food industry to extend the microbiological shelf-life of meat. typically, poultry meat has been packaged in a co2/n2 atmosphere (with residual low o2). recently, some producers use high o2 map for poultry meat to empirically reach comparable shelf lifes. in this work, we compared spoilage microbiota of skinless chicken breast in high (80% o2, 20% co2) and low o2 map (65% n2 and 35% co2). two batches of meat were incubated in each atmosphere ...201627554149
mycobacterial antigen 85 complex (ag85) as a target for ficolins and mannose-binding lectin.the pattern recognition molecules (prms) able to activate complement via the lectin pathway are suspected to be involved in the interaction between pathogenic mycobacteria and the host immune response. recently, we have found strong interactions between 25 and 35kda mycobacterial cell fractions and mannose-binding lectin (mbl) and ficolins. here we demonstrate that two biologically important mycobacterial structures, mannosylated lipoarabinomannan (manlam) and the antigen 85 (ag85) complex, indu ...201627141819
antimicrobial resistance patterns of enterobacteriaceae in european wild bird species admitted in a wildlife rescue centre.wild birds have been considered to be reservoirs of enteric human pathogens and vectors of resistance dissemination to the environment. during annual migration, they potentially play a role in the epidemiology of human associated zoonoses. the aim of this study was to investigate the frequency of isolation and antimicrobial susceptibility profiles of enterobacteriaceae members isolated from cloacal swabs of common european wild birds. fifty-five cloacal swabs were taken during birds' entrance ev ...201627393875
periodontal-disease-associated biofilm: a reservoir for pathogens of medical importance.the ecological diversity of the periodontal microenvironment may provide suitable conditions for the colonization of species not usually considered members of the oral microbiota. in this investigation, we aimed to determine the prevalence and levels of pathogenic species of medical relevance in the microbiota of individuals with distinct periodontal clinical status. subgingival biofilm was obtained from patients with periodontal health (h, n = 81), gingivitis (g, n = 55), generalized aggressive ...201626416306
survival of cheese-ripening microorganisms in a dynamic simulator of the gastrointestinal tract.a mixture of nine microorganisms (six bacteria and three yeasts) from the microflora of surface-ripened cheeses were subjected to in vitro digestive stress in a three-compartment "dynamic gastrointestinal digester" (didgi). we studied the microorganisms (i) grown separately in culture medium only (ii) grown separately in culture medium and then mixed, (iii) grown separately in culture medium and then included in a rennet gel and (iv) grown together in smear-ripened cheese. the yeasts geotrichum ...201626611167
the effect of reduced sodium chloride content on the microbiological and biochemical properties of a soft surface-ripened cheese.many health authorities have targeted salt reduction in food products as a means to reduce dietary sodium intake due to the harmful effects associated with its excessive consumption. in the present work, we evaluated the effect of reducing sodium chloride (nacl) content on the microbiological and biochemical characteristics of an experimental surface-ripened cheese. a control cheese (1.8% nacl) and a cheese with a reduced nacl content (1.3% nacl) were sampled weekly over a period of 27d. reducin ...201626852809
control of shigatoxin-producing escherichia coli in cheese by dairy bacterial strains.bio-preservation could be a valuable way to control shigatoxin-producing escherichia coli (stec) in cheese. to this end, 41 strains were screened for their inhibitory potential on model cheese curd and on pasteurized and raw milk uncooked pressed cheeses. strains of lactococcus lactis, lactococcus garvieae, leuconostoc pseudomesenteroides, leuconostoc citreum, lactobacillus sp, carnobacterium mobile, enterococcus faecalis, enterococcus faecium, macrococcus caseolyticus and hafnia alvei reduced s ...201626678131
fractionation and analysis of lipopolysaccharide-derived oligosaccharides by zwitterionic-type hydrophilic interaction liquid chromatography coupled with electrospray ionisation mass spectrometry.lipopolysaccharide (lps, endotoxin) is a main surface antigen and virulence factor of gram-negative bacteria. regardless of the source of lps, this molecule, isolated from the smooth forms of bacteria, is characterised by a general structural layout encompassing three regions: (i) an o-specific polysaccharide (o-ps) - a polymer of repeating oligosaccharide units, (ii) core oligosaccharide (os), and (iii) the lipid a anchoring lps in the outer membrane of the cell envelope of gram-negative bacter ...201627085741
[morpho-functional characteristic of enterobacteria adhesive activity].study adhesive activity of enterobacteria in the model of hemagglutination reaction with animal and avian erythrocytes and clarify structures responsible for adhesion in enterobacteria.201626829846
a broadband capacitive sensing method for label-free bacterial lps detection.in this paper, the authors present a new type of highly sensitive label-free microwave sensor in a form of interdigital capacitor coated with t4 bacteriophage gp37 adhesin. the adhesin binds escherichia coli b (e. coli b) by precise recognizing its bacterial host lipopolysaccharide (lps). the c-terminal part of the adhesin consists of the receptor-binding amino acid residues which are involved in a specific interaction with two terminal glucose residues of the bacterial lps. the change of the se ...201626339930
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