Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| ultrastructure of the capsule of klebsiella pneumoniae and slime of enterobacter aerogenes revealed by freeze etching. | 1973 | 4591533 | |
| glutamine synthetase and the regulation of histidase formation in klebsiella aerogenes. | 1973 | 4145798 | |
| regulation of the synthesis of enzymes responsible for glutamate formation in klebsiella aerogenes. | 1973 | 4146916 | |
| [biosynthesis of indole in not aerated cultures of klebsiella aerogenes (author's transl)]. | 1973 | 4147736 | |
| the function of ubiquinone in klebsiella aerogenes. | 1973 | 4148761 | |
| diacetyl (acetoin) reductase from aerobacter aerogenes. kinetic mechanism and regulation by acetate of the reversible reduction of acetoin to 2,3-butanediol. | 1973 | 4144829 | |
| the participation of cytochromes in the process of nitrate respiration in klesbsiella (aerobacter) aerogenes. | 1973 | 4145134 | |
| effect of methionine sulfoximine and methionine sulfone on glutamate synthesis in klebsiella aerogenes. | at least two pathways exist in klebsiella aerogenes for glutamate synthesis. a mutant blocked in one pathway due to the loss of glutamate dehydrogenase (gltd) does not require glutamate and has the same growth characteristics as the parent strain in most media; however, its growth is inhibited by the analogues methionine sulfoximine and methionine sulfone. wild-type klebsiella is resistant to 0.1 m methionine sulfoximine or methionine sulfone, whereas the gltd mutant is sensitive to 1 mm concent ... | 1973 | 4145197 |
| biomagnification of p, p'-ddt and methoxychlor by bacteria. | aerobacter aerogenes and bacillus subtilis accumulated p, p'-ddt and methoxychlor directly from water. uptake of both (14)c-labeled organochlorine insecticides was rapid; 80 to 90% of the 24-h residues were reached within 30 min. total cellular residues varied linearly with concentrations of ddt and methoxychlor in water ranging from 0.5 to 5.0 mug/liter. the residue magnification factors from water were between 1,400- to 4,300-fold, but were independent of insecticide concentrations in water. w ... | 1973 | 4199339 |
| hydroxamate recognition during iron transport from hydroxamate-ion chelates. | kinetics of radioactive iron transport from three structurally different secondary hydroxamate-iron chelates (schizokinen-iron, produced by bacillus megaterium atcc 19213; desferal-iron, produced by an actinomycete; and aerobactin-iron, produced by aerobacter aerogenes 62-1) revealed that b. megaterium sk11 (a mutant which cannot synthesize schizokinen) has a specific transport system for utilization of ferric hydroxamates with a recognition capacity based on the chemical structure of the hydrox ... | 1973 | 4199516 |
| fate of labeled hydroxamates during iron transport from hydroxamate-ion chelates. | the fate of the hydroxamic acid-iron transport cofactors during iron uptake from the (59)fe(3+) chelates of the (3)h-labeled hydroxamates schizokinen and aerobactin was studied by assay of simultaneous incorporation of both (59)fe(3+) and (3)h. in the schizokinen-producing organism bacillus megaterium atcc 19213 transport of (59)fe(3+) from the (3)h-schizokinen-(59)fe(3+) chelate at 37 c was accompanied by rapid uptake and release (within 2 min) of (3)h-schizokinen, although (3)h-schizokinen dis ... | 1973 | 4199517 |
| the lactose system in klebsiella aerogenes v9a. 3. specific repression of the lac operon by melibiose and raffinose. | 1973 | 4360018 | |
| butanol-soluble glycosyl transferases in klebsiella aerogenes. | 1973 | 4355907 | |
| letter: a phosphodiesterase from enterobacter aerogenes. | 1973 | 4357681 | |
| amino acid sequence studies with the tryptophan synthetase chain of aerobacter aerogenes. | 1973 | 4571778 | |
| comparative resistance of nonsporogenic bacteria to low-temperature gamma irradiation. | a total of 36 microorganisms, comprising 19 species of 11 genera, were screened for radiation resistance with (60)co gamma rays at a radiation temperatore of -80 +/- 2 c in phosphate buffer (ph 7.0) under vacuum. micrococcus radiodurans was the most resistant organism. an initial population of 2.8 x 10(5) cells per dose of this species survived 2.4 but not 2.7 mrad. of the remaining 18 species with initial populations of about 10(6) cells per dose, streptococcus faecium survived 0.9 to 1.5 mrad, ... | 1973 | 4572982 |
| nucleotide sequence divergence in the -chain-structural genes of tryptophan synthetase from escherichia coli, salmonella typhimurium, and aerobacter aerogenes. | two different estimates were obtained for the extent of nucleotide sequence divergence in the structural genes of the tryptophan synthetase alpha-chains of escherichia coli, salmonella typhimurium, and aerobacter aerogenes. one estimate was based on comparisons of the amino acid sequences of the respective alpha chains. the other was derived from measurements of the thermal stability of rna-dna hybrids formed with phage dna carrying the alpha-chain structural gene of e. coli and labeled messenge ... | 1973 | 4577791 |
| evaluation of radiometric system for detecting bacteremia. | an automated radiometric system (bactec, johnston laboratories) for detection of bacteremia was evaluated in parallel with a standard blood culture system in use in our laboratory. of 1,445 blood cultures from 484 patients with possible bacteremia, 106 sets of cultures (excluding 39 presumed contaminated), representing 56 patients, were positive by both methods. the conventional system yielded 85 positive cultures from 48 patients, whereas the bactec system yielded 84 positive cultures from 43 p ... | 1973 | 4584580 |
| diacetyl (acetoin) reductase from aerobacter aerogenes. kinetic studies of the reduction of diacetyl to acetoin. | 1973 | 4349658 | |
| formation of 2,3-pentanediol from 2,3-pentanedione and acetylethylcarbinol by diacetyl(acetoin)reductase from aerobacter aerogenes. a possible new pathway. | 1973 | 4350968 | |
| unimpaired de novo synthesis of 4-aminobenzoate in a mutant of aerobacter aerogenes requiring 4-aminobenzoate for growth; structure of compond a. | 1973 | 4272305 | |
| molecular characterization of the r factors implicated in the carbenicillin resistance of a sequence of pseudomonas aeruginosa strains isolated from burns. | an outbreak of r-factor-mediated carbenicillin resistance in pseudomonas aeruginosa in burned patients in march 1969 was followed by a second outbreak 6 months later. no r-factor-carrying p. aeruginosa strains were detected in the intervening period but r-factor-determined lactamase was commonly encountered, particularly in klebsiella aerogenes strains. a comparison of the molecular properties of the r factors in pseudomonads from the first and second phases with those in the klebsiella strains ... | 1973 | 4208284 |
| citrate metabolism in aerobacter cloacae. | growth of aerobacter cloacae on citrate either anaerobically or aerobically did not require and was not stimulated by the presence of na(+) in the medium. citrate was metabolized anaerobically via the fermentation pathway as evidenced by the (i) presence of oxalacetate decarboxylase, (ii) induction of citrate lyase, and (iii) repression of alpha-ketoglutarate dehydrogenase under anaerobic conditions. thus, although all the other enzymes of the citric acid cycle were present in anaerobic cells, t ... | 1974 | 4211873 |
| newer aminoglycosides--amikacin and tobramycin: an in-vitro comparison with kanamycin and gentamicin. | tobramycin and amikacin are new aminoglycoside antibiotics which resemble gentamicin and kanamycin respectively in pharmacokinetic, toxicological, and some microbiological respects. minimum inhibitory concentrations for these four antibiotics have been determined against 393 bacterial strains (13 species from eight genera) isolated from clinical materialkanamycin was the least active, 89% of the pseudomonas aeruginosa strains being resistant. resistance was also found in 21% of the klebsiella ae ... | 1974 | 4213265 |
| effect of ethylenediaminetetraacetic acid and related chelating agents on whole cells of gram-negative bacteria. | the effects of ethylenediaminetetraacetic acid and of four other chelating agents on seven strains of gram-negative bacteria are described. changes in viability, cell lysis, and release of intracellular materials from suspensions in various buffers, ph 7.8 or 9.2, were observed. cyclohexane-1,2-diaminotetraacetic acid was the most toxic compound, whereas iminodiacetic acid and nitrilotriacetic acid had little bactericidal activity. the activity of the five drugs appeared to be related to their c ... | 1974 | 4218945 |
| pressure transducer method for measuring gas production by microorganisms. | a simple method for measuring gas production by microorganisms by using a pressure transducer to sense pressure buildup was developed and tested with members of the coliform group. the test system consisted of a 5.0 lb/in(2) pressure transducer and a pressure equalizer valve attached to the metal cap of a test tube (20 by 150 mm); gas pressure was recorded on a strip-chart recorder. gas pressure response curves consisted of (i) a lag period with no marked increase in pressure, (ii) a rapid press ... | 1974 | 4589123 |
| [klebsiella aerogenes as the causative agent of postabortion sepsis complicated by acute renal insufficiency]. | 1974 | 4409939 | |
| nonidentical subunits of citrate lyase from klebsiella aerogenes. | 1974 | 4414837 | |
| proceedings: klebsiella aerogenes. infections in the tropics. | 1974 | 4415279 | |
| stereospecificity of the myo-inositol transport system of aerobacter aerogenes and sensitivity towards sulfhydryl reagents. | 1974 | 4415893 | |
| effect of sodium on the transport and utilization of citric acid by aerobacter (enterobacter) aerogenes. | sodium inhibited citrate uptake by two of the four strains of aerobacter (enterobacter) aerogenes used in these studies, had no effect on one strain, and stimulated citrate uptake by one strain. two of the four strains grew well anaerobically on citrate in the presence of na(+), one grew poorly, and one grew not at all either in the presence or absence of na(+). na(+) stimulated the aerobic growth of one strain on citrate, increased the total growth but not the rate of growth of one strain, and ... | 1974 | 4418533 |
| [study of purine analogs of cobamide coezyme in a glycerol dehydratase system from aerobacter aerogenes]. | 1974 | 4455705 | |
| proceedings: the respiratory nitrate reductase of klebsiella aerogenes: composition and the role of metal groups. | 1974 | 4461583 | |
| methods for visualization of enzymes in polyacrylamide gels. | white bands resulting from precipitation of dodecan-1-ol liberated by hydrolysis of sodium dodecyl sulfate and decan-5-ol released by hydrolysis of decan-5-yl sulfate produced zymograms of the primary and secondary alkylsulfatases from pseudomonas c(12)b. gas-liquid chromatographic analyses of ether extracts of the precipitate-containing segments of the zymograms confirmed the identity of the alcohols which were not discerned in extracts of segments of the gels other than those containing precip ... | 1974 | 4358859 |
| amino-terminal sequence of the tryptophan synthetase alpha chain of bacillus subtilis. | the sequence of the 46 nh(2)-terminal residues of the tryptophan synthetase alpha chain of bacillus subtilis was determined and compared with the corresponding sequences of escherichia coli, shigella dysenteriae, salmonella typhimurium, aerobacter aerogenes, serratia marcescens, and pseudomonas putida. a deletion of six residues was found at the nh(2)-terminal end of the alpha chain of b. subtilis. | 1974 | 4206869 |
| purification and kinetic characterization of a monovalent cation-activated glycerol dehydrogenase from aerobacter aerogenes. | 1974 | 4364415 | |
| mechanism of enzymic acetylation of des-acetyl citrate lyase. | a new enzyme, acetate:sh-[acyl-carrier-protein] enzyme ligase (amp), has been purified about 30-fold from cell-free extracts of klebsiella aerogenes. the enzyme, in the presence of acetate and atp, catalyzes the acetylation of enzymically inactive des-acetyl citrate lyase to enzymically active citrate lyase (ec 4.1.3.6). acetate and atp could be replaced by acetyl adenylate. acetyl-coa can act as acetyl donor in this activation only if trace amounts of adenine nucleotides and auxiliary enzymes a ... | 1974 | 4365579 |
| ribitol catabolic pathway in klebsiella aerogenes. | in klebsiella aerogenes w70, there is an inducible pathway for the catabolism of ribitol consisting of at least two enzymes, ribitol dehydrogenase (rdh) and d-ribulokinase (drk). these two enzymes are coordinately controlled and induced in response to d-ribulose, an intermediate of the pathway. whereas wild-type k. aerogenes w70 are unable to utilize xylitol as a carbon and energy source, mutants constitutive for the ribitol pathway are able to utilize rdh to oxidize the unusual pentitol, xylito ... | 1974 | 4366025 |
| d-arabitol catabolic pathway in klebsiella aerogenes. | klebsiella aerogenes strain w70 has an inducible pathway for the degradation of d-arabitol which is comparable to the one found in aerobacter aerogenes strain prl-r3. the pathway is also similar to the pathway of ribitol catabolism in that it is composed of a pentitol dehydrogenase, d-arabitol dehydrogenase (adh), and a pentulokinase, d-xylulokinase (dxk). these two enzymes are coordinately controlled and induced in response to d-arabitol, the apparent inducer of synthesis of these enzymes. we o ... | 1974 | 4366026 |
| regulation of glycerol catabolism in klebsiella aerogenes. | the utilization of glycerol as a carbon source for growth by klebsiella aerogenes, strain 2103, involves separate aerobic (sn-glycerol-3-phosphate or g3p) and anaerobic (dihydroxyacetone or dha) pathways of catabolism. enzyme and transport activities of the aerobic pathway are elevated in cells grown under oxygenated conditions on glycerol or g3p. anaerobic growth on g3p as carbon source requires the presence of an exogenous hydrogen acceptor such as fumarate; cells thus grown also are highly in ... | 1974 | 4366250 |
| close genetic linkage of the determinants of the ribitol and d-arabitol catabolic pathways in klebsiella aerogenes. | klebsiella aerogenes strain w70 has separate inducible pathways for the degradation of the pentitols ribitol and d-arabitol. these pathways are closely linked genetically as determined by transduction with phage pw52. there are two regulatory sites for the ribitol catabolic pathway as defined by loci for mutations to constitutive synthesis of ribitol dehydrogenase and d-ribulokinase, rbtb and rbtc. the two control sites are separated by a site represented by the dalb22 mutation. this mutation de ... | 1974 | 4366363 |
| [klebsiella aerogenes infection in turkey chicks and therapeutic attempts using polymyxin b]. | 1974 | 4368172 | |
| isoprenoid alcohol kinase--a third butanol-soluble enzyme in klebsiella aerogenes membranes. | 1974 | 4373523 | |
| catabolite repression and derepression of arylsulfatase synthesis in klebsiella aerogenes. | when a mutant (mao(-)) of klebsiella aerogenes lacking an enzyme for tyramine degradation (monoamine oxidase) was grown with d-xylose as a carbon source, arylsulfatase was repressed by inorganic sulfate and repression was relieved by tyramine. when the cells were grown on glucose, tyramine failed to derepress the arylsulfatase synthesis. when grown with methionine as the sole sulfur source, the enzyme was synthesized irrespective of the carbon source used. addition of cyclic adenosine monophosph ... | 1974 | 4376143 |
| a comparison of wild-type and mutant ribitol dehydrogenases from klebsiella aerogenes. | a ribitol dehydrogenase (ribitol-nad(+) oxidoreductase, ec. 1.1.1.56) having increased specificity and catalytic efficiency toward xylitol was isolated from mutant strains of klebsiella aerogenes, which were selected for increased growth rate on xylitol over the ribitol dehydrogenase constitutive wild-type organism. 2. the mutant enzyme was purified to homogeneity and its general characteristics were compared with those of the previously purified wild-type enzyme. 3. initial-velocity steady-stat ... | 1974 | 4376942 |
| pullulanase synthesis in klebsiella (aerobacter) aerogenes strains growing in continuous culture. | 1. pullulanase synthesis was studied in 16 classified (n.c.i.b.) strains and in an industrial strain (r) of klebsiella aerogenes grown in chemostats containing maltose as inducer and sole carbon source. 2. maximum synthesis was associated with carbon-limited growth at a low dilution rate (about 0.2h(-1)). the enzyme remained firmly cell-bound and seemed to be located on the cell surface. 3. three strains had high activity (r, n.c.i.b. 5938, 8017), twelve were intermediate, and two (n.c.i.b. 8153 ... | 1974 | 4376962 |
| mutants of klebsiella aerogenes lacking glutamate dehydrogenase. | a mutant of klebsiella aerogenes lacking glutamate synthase activity (asm-200) is blocked in only one pathway of glutamate synthesis and can still use glutamate dehydrogenase to produce glutamate when ammonia in sufficient concentration, i.e., higher than 1 mm, is provided in the medium. however, a mutant that has neither glutamate synthase nor glutamate dehydrogenase activities (asm-200, gdhd1) requires glutamate. transductants obtained by phage grown on wild-type cells of this double mutant, s ... | 1974 | 4149514 |
| [lack of reproduction in certain enterobacter aerogenes strains producing fraction 2 aerocines]. | 1974 | 4151210 | |
| different effects of 2-n-heptyl-4-hydroxyquinoline-n-oxide on oxygen and nitrate respiration in klebsiella aerogenes. | 1974 | 4155268 | |
| regulation of nitrogen fixation in klebsiella pneumoniae: evidence for a role of glutamine synthetase as a regulator of nitrogenase synthesis. | mutations causing constitutive synthesis of glutamine synthetase (glnc(-) phenotype) were transferred from klebsiella aerogenes into klebsiella pneumoniae by p1-mediated transduction. such glnc(-) strains of k. pneumoniae have constitutive levels of glutamine synthetase. two of three glnc(-) strains of k. pneumoniae studied, each containing independently isolated mutations that confer the glnc(-) phenotype, continue to synthesize nitrogenase in the presence of nh(4) (+). one strain, kp5069, prod ... | 1974 | 4156359 |
| evaluating the membrane fecal coliform test by using escherichia coli as the indicator organism. | the fecal coliform membrane filter method (mfc) currently used in water pollution analysis was evaluated by using two strains of escherichia coli, a known fecal coliform, as the indicator organism. a large relative error in the results obtained with this method was found to be dependent upon the brand of membrane filter employed, the medium, and the temperature of incubation. mfc densities varied between 10 and 60% of the densities determined by means of total bacteria counts and total coliform ... | 1974 | 4596755 |
| subphrenic abscess: comparison between operative and antibiotic management. | subphrenic abscess is still a significant hazard which complicates surgical procedures as well as certain abdominal catastrophes. this is a report of 88 patients with subphrenic abscess at st. vincent's hospital and medical center of new york from 1954 through 1971. there were 46 males and 42 females, ranging from 2 to 88 years. operations on the stomach, duodenum and biliary tract were the major causes. the causative organisms in order of frequency were: e coli (41.6%), staphylococcus (41.6%), ... | 1974 | 4601985 |
| procaryote phylogeny. i. concerning the relatedness of aerobacter aerogenes to escherichia coli. | 1974 | 4606938 | |
| the lactose system in klebsiella aerogenes v9a. 4. a comparison of the lac operons of klebsiella and escherichia coli. | 1974 | 4618214 | |
| ribitol dehydrogenase from klebsiella aerogenes. purification and subunit structure. | ribitol dehydrogenase has been purified to homogeneity from several strains of klebsiella aerogenes. one strain yields 3-6g of pure enzyme from 1kg of cells. the enzyme is a tetramer of four subunits, mol.wt. 27000. preliminary studies of the activity of the enzyme are reported. peptide ;maps' together with the amino acid composition indicate that the subunits are identical. | 1974 | 4618776 |
| a mass-spectrometric sequence study of the enzyme ribitol dehydrogenase from klebsiella aerogenes. | the first detailed results of the application of a low-resolution mixture analysis approach to the sequence analysis of an enzyme, ribitol dehydrogenase, are given. examples of the interpretation of the spectra of peptide mixtures derived from this protein are described. evidence for new fragmentation patterns observed is reported, together with an explanation of the generation of ambiguous sequences by use of a low-specificity enzyme, thermolysin. the overall sequencing strategy evolved is asse ... | 1974 | 4618777 |
| [isolation of aerobacter aerogenes from pozol of the state of campeche]. | 1974 | 4526609 | |
| glutamine-binding subunit of glutamate synthase and partial reactions catalyzed by this glutamine amidotransferase. | in the course of studies on glutamine-dependent carbamyl phosphate synthetase from aerobacter aerogenes, we purified another protein which was found to be glutamate synthase (ec 2.6.1.53). the enzyme, obtained in apparently homogeneous form (monomer molecular weight about 227,000; s(20,omega) = 17.6 s), was found to be a typical glutamine amidotransferase in that it exhibits glutaminase activity and can utilize ammonia in place of glutamine as a nitrogen donor. the enzyme also catalyzes at low r ... | 1974 | 4531004 |
| genetic transfer of nitrogen fixation from rhizobium trifolii to klebsiella aerogenes. | 1974 | 4828191 | |
| distribution of the isopropylmalate pathway to leucine among diverse bacteria. | alpha-isopropylmalate synthase and beta-isopropylmalate dehydrogenase activities were detected in extracts of the following organisms: chromatium d, rhodopseudomonas spheroides, hydrogenomonas h16, pseudomonas aeruginosa, pseudomonas fluorescens, vibrio extorquens, rhizobium japonicum, alcaligenes viscolactis, escherichia coli b, proteus vulgaris, aerobacter aerogenes, salmonella typhimurium, micrococcus sp., micrococcus lysodeikticus, bacillus polymyxa, bacillus subtilis, and nocardia opaca. th ... | 1974 | 4829932 |
| effect of complex nutrients, vitamins and amino acids on valine production by aerobacter aerogenes. | 1974 | 4836928 | |
| synthesis of s-alkyl-l-cysteine from pyruvate, ammonia and alkyl-mercaptan by cysteine desulfhydrase of aerobacter aerogenes. | 1974 | 4854588 | |
| cefamandole, a cephalosporin antibiotic with an unusually wide spectrum of activity. | the in vitro activity of cefamandole, 7-d-mandelamido-3-(1 methyl-1h-tetrazol-5 yethiomethyl)-3-cephem-4 carboxylic acid, was investigated. the majority of streptococci and pneumococci were inhibited by 0.1 mug/ml. activity against staphylococcus aureus was below that of cephalothin, but most strains were inhibited by 0.4 mug/ml. enterococci were not inhibited by less than 25 mug/ml. cefamandole was very active against most members of the enterobacteriaceae, with 70% of escherichia coli, 86% of ... | 1974 | 15828189 |
| the regulation of carbohydrate metabolism in klebsiella aerogenes nctc 418 organisms, growing in chemostat culture. | klebsiella aerogenes nctc 418 was grown in chemostat cultures (d = 0.17 hr-1; ph 6.8;35 degrees c) that were, successively, carbon-, sulphate-, ammonia-, and phosphate-limited, and which contained as the sole carbon-substrate first glucose, then glycerol, mannitol and lactate. quantitative analyses of carbon-substrate used and products formed allowed carbon balances to be constructed and direct comparisons to be made of the efficiency of substrate utilzation. with all sixteen cultures, carbon re ... | 1975 | 766718 |
| inactivation of citrate lyase from rhodopseudomonas gelatinosa by a specific deacetylase and inhibition of this inactivation by l-(+1-glutamate. | a previously unrecognized enzyme, citrate lyase deacetylase, has been purified about 140-fold from cell extracts of rhodopseudomonas gelatinosa. it catalyzed the conversion of enzymatically active acetyl-s-citrate lyase into the inactive hs-form and acetate. the enzyme exhibited an optimal rate of inactivation at ph 8.1. because of the instability of acetyl-s-citrate lyase at acidic and alkaline ph values, all assays were carried out at ph 7.2, where the spontaneous hydrolysis of the acetyl-s-ci ... | 1975 | 356 |
| expression of the hut operons of salmonella typhimurium in klebsiella aerogenes and in escherichia coli. | the normal hut (histidine utilization) operons, as well as those with mutations affecting the regulation of their expression, of salmonella typhimurium were introduced on an f' episome into cells of s. typhimurium and klebsiella aerogenes whose chromosomal hut genes had been deleted and into cells of escherichia coli, whose chromosome does not carry hut genes. the episomal hut operons respond in a manner very similar to induction and catabolite repression in all three organisms. the small differ ... | 1975 | 362 |
| regulation of the hut operons of salmonella typhimurium and klebsiella aerogenes by the heterologous hut repressors. | in merodiploid strains of klebsiella aerogenes with chromosomal hut genes of k. aerogenes and episomal hut genes of salmonella typhimurium, the repressor of either species can regulate the hut operons of the other species. the repression exerted by the homologous repressor on the left-hand hut operon is, in both organisms, stronger than that exerted by the heterologous repressor. | 1975 | 363 |
| aerobacter aerogenes prl-r3 urease. purification and properties. | a method for the preparation of a 150-fold purified and homogenous a. aerogenes urease is reported. the enzyme exhibited two ph optima at ph 7.0 and 7.5 in triethanolamine and phosphate buffer, respectively. the affinity of the enzyme toward its substrate increased with the increase of ph. no effect of the ph was observed on the measured temperature coefficient (q10). there was no discontinuity in the arrhenius plots at ph 5.4 and 7.5 but an upward discontinuity at ph 6.15 and 8.7 with transitio ... | 1975 | 879 |
| purification and some properties of a novel maltohexaose-producing exo-amylase from aerobacter aerogenes. | maltohexaose producing amylase (ec 3.2.1.-) is the fourth known exo-amylase, the three previously known being glucoamylase, beta-amylase and pseudomonas stutzeri maltotetraose producing amylase. the enzyme after release from aerobacter aerogenes cells by 0.1% sodium lauryl sulfate extraction was purified by ammonium sulfate precipitation, deae-sephadex column chromatography and sephadex g-100 gel filtration to 80-fold of the original sodium lauryl sulfate extract activity, it gave a single band ... | 1975 | 1094 |
| regulation of synthesis of glutamine synthetase by adenylylated glutamine synthetase. | we have examined three mutants of klebsiella aerogenes whose genetic lesions (glnb, glnd, and glne) are in loci unlinked to the structural gene for glutamine sythetase (glna) and in which the control of both the level and state of adenylylation of glutamine synthetase is altered. each mutation alters a different component of the adenylylation system of glutamine synthetase l-glutamate:ammonia ligase (adp-forming), ec 6.3.1.2. inability of the cell to deadenylylate glutamine synthetase (glnb and ... | 1975 | 1744 |
| immunological study of anthranilate synthetase. | an immunological study of anthranilate synthetase (asase) has been initiated using quantitative precipitation, enzyme neutralization, and immunodiffusion methods. cross-reactivity of anthranilate synthetase-anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase (asase-prtase) from escherichia coli, klebsiella aerogenes, and salmonella typhimurium and asase from serratia marcescens and pseudomonas putida was detected with antibodies to ?e. coli trypsin-treated asase. cross-reactivit ... | 1975 | 50316 |
| purification and properties of a phosphohydrolase from enterobacter aerogenes. | a phosphohydrolase from enterobacter aerogenes which hydrolyzes phosphate mono- and diesters has been purified approximately 50-fold to apparent homoeneity and crystallized. the enzyme is produced when the bacteria utilize phosphate diesters as sole phosphorus source. from sedimentation equilibrium experiments the molecular weight of the native enzyme is 173,000; from sodium dodecyl sulfate polyacrylamide gel electrophoresis the subunit molecular weight is 29,000, indicating that the enzyme is h ... | 1975 | 168197 |
| the enthalpies of hydrolysis of acyclic, monocyclic, and glycoside cyclic phosphate diesters. | the enthalpies of hydrolysis of acyclic, monocyclic, and glycoside cyclic phosphate diesters have been measured by flow microcalorimetry using a phosphohydrolase isolated from enterobacter aerogenes as catalyst. the values so obtained (kilocalories per mol) (at 25 degrees) for sodium salts are: diethyl phosphate, minus 1.8 plus or minus 0.5; ethylene phosphate, minus 6.4 plus or minus 0.2; trimethylene phosphate, minus 3.0 plus or minus 0.2; tetramethylene phosphate, minus 2.2 plus or minus 0.1; ... | 1975 | 168198 |
| independent constitutive expression of the aerobic and anaerobic pathways of glycerol catabolism in klebsiella aerogenes. | klebsiella aerogenes dissimilates glycerol aerobically via an inducible pathway initiated by an adenosine triphosphate-linked kinase that converts the substrate to sn-glycerol 3-phosphate. phosphorylated glycerol is then dehydrogenated to dihydroxyacetone phosphate by an enzyme characteristic of a flavoprotein. anaerobically, the organism dissimilates glycerol via an inducible pathway initiated by a nicotinamide adenine dinucleotide-linked dehydrogenase that converts the substrate to dihydroxyac ... | 1975 | 170247 |
| characterization of the respiratory nitrate reductase of klebsiella aerogenes as a molybdenum-containing iron-sulfur enzyme. | 1. in respiratory nitrate reductase i of klebsiella aerogenes, 0.24 atom of molybdenum, eight iron-sulfur groups and four tightly bound, non-heme iron atoms per molecule of enzyme (mr 260 000) are found. 2. epr spectra at 83 degrees k of oxidized and reduced nitrate reductase i show complex lines at g = 2.02 and g = 1.98, which are more intense in the reduced than in the oxidized enzyme. the resonances, the shape and intensity of which are rather temperature insensitive, are attributed to two sp ... | 1975 | 170983 |
| purification, structure and properties of the respiratory nitrate reductase of klebsiella aerogenes. | 1. the respiratory nitrate reductase of klebsiella aerogenes was solubilized from the bacterial membranes by deoxycholate and purified further by means of gel chromatography in the presence of deoxycholate, and anion-exchange chromatography. 2. dependent on the isolation procedure two different homogeneous forms of the enzyme, having different subunit compositions, can be obtained. these forms are designated nitrate reductase i and nitrate reductase ii. both enzyme preparations are isolated as t ... | 1975 | 234757 |
| identification of the structural gene for glutamine synthetase in klebsiella aerogenes. | mutations at two sites of the klebsiella aerogenes chromosome, unlinked by transduction with phages pw52 and p1, result in the lack of enzymatically active glutamine synthetase. a mutation in the glnb site leads to a marked decrease in the formation of an apparently normal enzyme. some of the mutations in the glna site lead to the production of enzymatically inactive material capable of reacting with anti-glutamine synthetase serum. the revertant of a glna mutant was found to produce a glutamine ... | 1975 | 234938 |
| genetic control of glutamine synthetase in klebiella aerogenes. | mutations at two sites, glna and glnb, of the klebsiella aerogenes chromosome result in the loss of glutamine synthetase. the locations of these sites on the chromosome were established by complementation by episomes of escherichia coli and by determination of their linkage to other genetic sites by transduction with phage p1. the glnb gene is located at a position corresponding to 48 min on the taylor map of the e. coli chromosome; it is linked to trya, nadb, and gua. the glna gene is at a posi ... | 1975 | 234939 |
| analysis of bacterial biotin-proteins. | the biotin-protein populations in several bacterial strains were analyzed by solubilization of [3h]biotin-labeled cells with sodium dodecylsulfate followed by electrophoresis on polyacrylamide gels containing the detergent. a variety of patterns of biotin-labeled polypeptide chains was seen, ranging from a single biotin-protein in escherichia coli, corresponding to the biotin carboxyl carrier protein component of acetyl-coa carboxylase, to multiple species in enterobacter aerogenes, pseudomonas ... | 1975 | 235315 |
| l-ascorbic acid 2-sulphate. a substrate for mammalian arylsulphatases. | ascorbic acid 2-sulphate has a stability in acid comparable to that of phenyl sulphate and is rather more acid-labile than simple carbohydrate sulphates. at its optimum ph of 4.8 sulphatase a(aryl-sulphate sulphohydrolase ec 3.1.6.1.) hydrolyses ascorbic acid sulphate with a specific activity of 90 mumol/mg per min (150 mumol/mg per min with nitrocatechol sulphate at ph 5.6). at ph 4.8 the kinetics are non-michaelis. at ph 5.6 michaelis kinetics are obeyed and km 12 21 mm ascorbic acid 2-sulphat ... | 1975 | 235988 |
| effect of aeration and sodium on the metabolism of citrate by klebsiella aerogenes. | anaerobic growth of klebsiella aerogenes ncdo 711 (nctc 418) on citrate was dependent on the presence of na+ in the medium, and fermentation of citrate was mediated via the fermentation pathway enzymes, citrate lyase and a na+-dependent oxalacetate decarboxylase. this confirms the previous findings on strain nctc 418. growth under aerobic conditions was independent of na+. the mean generation time for cells grown aerobically on either na+ or k+ citrate medium was about 60 min, with a molar growt ... | 1975 | 236280 |
| impedance monitoring of bacterial activity. | bacterial activity and growth were monitored by following the changes of electrical impedance of cultures in liquid media. the signal is expressed automatically as a curve similar to grwoth curves produced by other methods. the technique offers a new, rapid and sensitive means of detecting active micro-organisms and is potentially the basis of rapid automated systems in this field. the impedance changes indicate that the micro-organisms metabolise substrates of low conductivity into products of ... | 1975 | 236390 |
| l-ribulose-5-phosphate 4-epimerase from aerobacter aerogenes. | 1975 | 236467 | |
| d-arabinose (l-fucose) isomerase from aerobacter aerogenes. | 1975 | 236476 | |
| 2,3-butanediol biosynthetic system in aerobacter aerogenes. | 1975 | 236481 | |
| glycerol dehydratase from aerobacter aerogenes. | 1975 | 237191 | |
| gene order of the histidine utilization (hut) operons in klebsiella aerogenes. | p1-sensitive mutants of klebsiella aerogenes were isolated and the gene order of the hut region was then determined using p1-mediated transduction. the genes are located in the klebsiella chromosome between gal and bio as in salmonella typhimurium. the gene order, gal, huti, hutg, hutc, huu, huth, bio is also the same as that observed in s. typhimurium. | 1975 | 238937 |
| regulation of nitrogen metabolism in escherichia coli and klebsiella aerogenes: studies with the continuous-culture technique. | ammonia-nitrogen-limited continuous cultures of escherichia coli and klebsiella aerogenes contain induced levels of glutamine synthetase that is deadenylyated (i.e., fully active). in the presence of excess ammonia or glutamate in glucose-limited cultures of e. coli, glutamine synthetase is repressed and adenylylated (inactive). the average state of adenylylation (n) is a linear function of the specific growth rate. at low specific growth rates, glutamine synthetase is adenylylated; as the speci ... | 1975 | 238954 |
| purification and properties of citrate lyase from streptococcus diacetilactis. | citrate lyase from streptococcus diacetilactis has been purified to yield a protein that was homogeneous as judged by sedimentation velocity and sedimentation equilibrium experiments. the enzyme's sedimentation coefficient is 16.8 s and its molecular weight is around 585,000. it contains three nonidentical subunits of about 53,000, 34,000, and 10,000 daltons. the enzyme in its active form contains an acetyl group which turns over during the citrate cleavage reaction. removal of the acetyl group ... | 1975 | 238987 |
| physiological and biochemical role of the butanediol pathway in aerobacter (enterobacter) aerogenes. | aerobacter (enterobacter) aerogenes wild type and three mutants deficient in the formation of acetoin and 2,3-butanediol were grown in a glucose minimal medium. culture densities, ph, and diacetyl, acetoin, and 2,3-butanediol levels were recorded. the ph in wild-type cultures dropped from 7.0 to 5.8, remained constant while acetoin and 2,3-butanediol were formed, and increased to ph 6.5 after exhaustion of the carbon source. more 2,3-butanediol than acetoin was formed initially, but after glucos ... | 1975 | 239921 |
| regulation of the ammonia assimilatory enzymes in salmonella typhimurium. | the regulation of glutamate dehydrogenase (ec 1.4.1.4), glutamine synthetase (ec 6.3.1.2), and glutamate synthase (ec 2.6.1.53) was examined for cultures of salmonella typhimurium grown with various nitrogen and amino acid sources. in contrast to the regulatory pattern observed in klebsiella aerogenes, the glutamate dehydrogenase levels of s. typhimurium do not decrease when glutamine synthetase is derepressed during growth with limiting ammonia. thus, it appears that the s. typhimurium glutamin ... | 1975 | 240804 |
| effect of different environmental factors on valine production by aerobacter aerogenes. | 1975 | 241175 | |
| [increased incidence of some strains of klebsiella aerogenes]. | study of the incidence of klebsiella aerogenes strains in neonates and infants with acute enterocolitis in the maternity and pediatric departments gave the following results: - bacteriologic investigations were carried out in 1831 subjects, isolating 138 klebsiella aerogenes strains; - the proportion of klebsiella isolations increased from 4.6% in 1973 to 11.1% in 1974; - the maximum receptivity to klebsiella aerogenes was noted in the 0-2 years age group, in a proportion of 23%; - in the 0-6 mo ... | 1975 | 128803 |
| improved large-volume sampler for the collection of bacterial cells from aerosol. | a modified large-volume sampler was demonstrated to be an efficient device for the collection of mono-disperse aerosols of rhodamine b and poly-disperse aerosols of bacterial cells. absolute efficiency for collection of rhodamine b varied from 100% with 5-mum particles to about 70% with 0.5-mum particles. the sampler concentrated the particles from 950 liters of air into a flow of between 1 and 2 ml of collecting fluid per min. spores of bacillus subtilis var. niger were collected at an efficien ... | 1975 | 803820 |
| herd studies on coliform mastitis. | escherichia coli, enterobacter aerogenes, and klebsiella pneumoniae were responsible for 63, 10, and 11 percent, respectively, of 158 coliform organisms recovered from mastitis cases in 8 california herds. the severity and nature of the coliform mastitis problem were found to vary greatly among herds but were characteristic for each of 4 herds studied in detail. | 1975 | 804464 |
| role of the tryptophan group in the action of pullulanase of aerobacter aerogenes. | 1. pullulanase [ec 3.2.1.4.] was inhibited by hg2+, n-bromosuccinimide, 2-hydroxy-5-nitrobenzyl bromide, riboflavine 5'-phosphate, histamine, imidazole, and carbodiimide. 2. the tryptophan groups of the pullulanase were modified by n-bromosuccinimede. it was found that one or two of the 25 tryptophan groups seemed to be important for the activity. 3. studies on difference spectra using various substrates suggested that the tryptophan groups were important for the formation of an enzyme-substrat ... | 1975 | 807566 |
| methyl violet: a selective agent for differentiation of klebsiella pneumoniae from enterobacter aerogenes and other gram-negative organisms. | three selective media for differentiation of klebsiella pneumoniae from enterobacter aerogenes on the basis of colonial morphology were evaluated. using methyl violet 2b as a selective agent, strains of k. pneumoniae isolated from urine, fresh water, and fresh produce were tested against other members of enterobacteriaceae in addition to strains of aeromonas hydrophila and pseudomonas aeruginosa. comparison of colonial morphology showed k. pneumoniae produced larger, smoother colonies than other ... | 1975 | 809008 |
| [vitamin b 12 dependent methionine biosynthesis in pseudomonas aeruginosa]. | among methionineless mutants of pseudomonas aeruginosa strain pao lacking the ability of methylating homocysteine we found two different types: one of them responding to methionine only, the other one to methionine or vitamin b12 alternatively. that means that p. aeruginosa pao as well as other b12-producing bacteria (with one exception: aerobacter aerogenes) use only the b12 pathway (meth) for methionine synthesis. the effect of some cys-auxotrophs equally growing on vitamin b12 as described by ... | 1975 | 809933 |
| susceptibilities of gram-negative bacteria to combinations of antimicrobial agents in vitro. | combinations of either clindamycin or erythromycin with gentamicin, colimycin, or carbenicillin were studied for their antimicrobial effects on clinical isolates of escherichia coli, klebsiella sp., proteus mirabilis, serratia marcescens, enterobacter aerogenes, and pseudomonas aeruginosa. no antagonistic effects of the antibiotic combinations were observed. synergistic effects of the antibiotic combinations were noted against most strains of e. coli tested, especially when either clindamycin or ... | 1975 | 810077 |
| a multigroup model for predator-prey interactions. | the predator-prey interactions between the protozoan tetrahymena pyriformis and the bacterium aerobacter aerogenes have been studied experimentally and mathematically. a mathematical model for the ciliates defines the mass distribution of cells within the population. the resulting model equations are solved by the use of multigroup theory. experimental data from batch and continuous flow rectors are compared with the results of the numerical integration. | 1975 | 810191 |