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purification, characterization and biological activity of islets activating protein (iap) from the supernatant fluids of bordetella pertussis cultures [proceedings]. 1978210305
[induction of antibodies to native dna by components of bordetella pertussis].administration of bordetella pertussis and some of their components to mice induced an increase of dna-binding activity of the sera revealed under ionic strength conditions of physiological saline, mostly on the 14th day. it was shown by the inhibition method that interaction between mouse sera and native dna was specific. maximum increase in the quantity of mouse sera proteins reacting with dna under low ionic strength condition of physiological saline (0.05 m nacl) is revealed on the 7th day. ...1978210865
vascular permeability changes in the central nervous system of rats with hyperacute experimental allergic encephalomyelitis induced with the aid of a substance from bordetella pertussis.development of hyperacute experimental allergic encephalomyelitis in lewis rats after intraperitoneal administration of a mixture of guinea pig spinal cord emulsion and pertussigen from bordetella pertussis was accompanied by an increase in vascular permeability in the central nervous system. the increased permeability was most striking in the spinal cord and seemed to be associated with the ascending development of paralysis. rats that had completely recovered from paralysis did not have any in ...1978211087
conversion of bordetella pertussis to bordetella parapertussis.the epidemiological and drug susceptibility data on whooping cough suggested a possibility that bordetella pertussis converts in some way to bordetella parapertussis. to prove this, b. pertussis strain 75 was treated with n-methyl-n'-nitro-n-nitrosoguanidine and a mutant resistant to staphcillin v and eight mutants resistant to trimethoprim were isolated. the staphcillin v-resistant mutant of b. pertussis agreed with all of the criteria of b. parapertussis and the trimethoprim-resistant mutants ...1978211161
[comparative study of lipid a from pertussis microbes differing in the toxicity of their o-antigens. i. chemical composition and stability of the bond between lipid a and the specific polysaccharide in b. pertussis lipopolysaccharide].data presented in this work indicated that antigens, contrastive by toxicity, obtained by boiven's method and o'neill and tood's method from two strains of bordetella pertussis differed by stability of lipid a binding with the specific polysaccharide. the influence of duration of the lipopolysaccharide hydrolysis on the fatty acid content in lipid a, and of heptose in the specific polysaccharide was demonstrated. lipid a fatty acid composition was studied. it is supposed that bound fatty acids a ...1978211760
protection of mice against viral infection by corynebacterium parvum and bordetella pertussis.mice could be significantly protected against infection with herpes simplex virus (hsv) by i.p. or i.v. injection of killed corynebacterium parvum 7 days before infection. this protection was seen in inbred strains of mice with a different degree of sensitivity to hsv and after both i.p. and i.v. infection. resistant mice immunosuppressed by x-irradiation and showing an increased susceptibility to hsv could also be protected by a previous injection of c. parvum. elevated levels of interferon wer ...1978212522
[morphological changes in the central nervous system of mice inoculated with a bordetella pertussis suspension]. 1978212699
bordetella pertussis serotypes in canada.a study was done to determine the major antigenic factors of bordetella pertussis strains isolated throughout canada and whether these isolates have the same antigenic structure as the bacilli in the currently used vaccines. testing for the major pertussis antigens, factors 1, 2 and 3, was conducted with 440 freshly isolated strains of b. pertussis received from seven canadian provinces between august 1976 and february 1978 and six batches of pertussis vaccine or immunizing agents containing per ...1978213182
characterization of mouse peritoneal exudate and associated leukocyte adherence inhibitory activity after intraperitoneal injection of either bordetella pertussis or corynebacterium parvum vaccines.bordetella pertussis and corynebacterium parvum are commonly used immunopotentiating agents. to explore the inflammatory environment induced by these agents, the peritoneal exudate response in mice following intraperitoneal injection of b. pertussis (pv) and c. parvum (cv) vaccines was investigated. the pv-induced exudate isolated by lavage was characterized by an early neutrophil influx followed by enhanced accumulation of mononuclear cells and fluid protein. the cv exudate was principally mono ...1978215552
effect of erythromycin and amoxycillin on bordetella pertussis in the nasopharynx.the effectiveness of oral erythromycin and amoxycillin in eradicating bordetella pertussis from the nasopharynx was compared. erythromycin in a dosage of 40--50 mg/kg/day was significantly more effective than amoxycillin in a dosage of 25--30 mg/kg/day. the organism did not disappear in three cases receiving a lower dosage of erythromycin. as antibiotic treatment does not affect the clinical course of fully-developed whooping cough, erythromycin is indicated primarily when particularly susceptib ...1978215555
action of phenylbutazone, cyclophosphamide and prednacinolone on pleurisy due to bordetella pertussis hypersensitivity in the rat.phenylbutazone, cyclophosphamide and prednacinolone acetonide, administered around the period of challenge reduced the exudate of pleurisy due to bordetella pertussis hypersensitivity in the rat. the results on the leukocytes of the exudate are different depending on the class of product studied. phenylbutazone only slightly reduced the number of mononuclears cells. both the immunosuppressive and the corticoid induced a clear decrease in the total leukocyte number. but, while prednacinolone effe ...1978216327
[protection against infection with bordetella pertussis by an oligopeptide from bordetella pertussis (author's transl)].we have isolated from bordetella pertussis an oligopeptide with characteristic amino acid composition. this peptide was applied to mice in standardized tests for pertussis immunization. in three tests with three independent isolates of peptide, a significant and dose dependent protection was observed. one microgram of peptide per mouse produces the same protective effect as 0.1 iu of pertussis vaccine. it is important to note that similar peptides can be isolated from other bacteria and other dn ...197877606
partial purification and some properties of inhibitors to the hemagglutinating activity of leukocytosis-promoting factor (lpf) from bordetella pertussis [proceedings]. 197879665
action of chloroquine on pleurisy due to bordetella pertussis hypersensitivity. 197829110
subunit structure of islets-activating protein (iap), a new protein isolated from the culture media of bordetella pertussis.the subunit structure was studied of islets-activating protein (iap), a new protein recently isolated from the culture media of bordetella pertussis and possessing a unique action, i.e., potentiating insulin secretory responses of animals, iap dissociated into three subunits, f-1, f-2, and f-3, when incubated in 8m urea. three subunits isolated by chromatography on cm-sepharose and deae-sepharose columns showed different molecular weights (f-1: 44,000, f-2: 20,000, f-3: 11,000) and different iso ...197829892
viruses and bacteria associated with acute respiratory illnesses in young children in general practice.the results obtained and the laboratory methods used for the isolation of viruses and bacteria from malaysian children with acute respiratory illnesses seen in a private clinic are described. of the 65 children studied virus isolations were obtained from 26 children, bacteria from 10 and both virus and bacteria from another 5. the agents isolated were influenza viruses, parainfluenza viruses, adenoviruses, bordetella pertussis, streptococcus pneumoniae, haemophilus influenzae and staphylococcus ...197830173
bordetella pertussis does not induce beta-adrenergic blockade.bordetella pertussis organisms induce histamine sensitivity and diminish the normal hyperglycemic response to epinephrine in experimental animals. these effects have been attributed to beta-adrenergic blockade. however, under conditions in which the decrease in epinephrine-induced hyperglycemia after b. pertussis administration was demonstrable, there was no change in rat reticulocyte beta-adrenergic receptor number or affinity measured by iodohydroxybenzylpindolol binding or in isoproterenol-st ...197832138
[opsonizing activity of sera from animals immunized with pertussis vaccine].opsonizing activity of guinea pig blood serum containing mercaptoethanol-resistant pertussis antibodies was studied in vitro on a model of microorganism ingestion by the mononuclears of the guinea pig peritoneal exudate. there were revealed distinct differences in the serum activity depending on the phagocytosis object. the blood serum of hyperimmunized rabbits stimulated the ingestion of bordetella pertussis by mononuclears of guinea pigs--normal and immunized with pertussis vaccine. the blood ...1978567917
effect of histamine on respiratory movements and isolated ileum of pertussis-vaccinated mice. a negative study.1. the sensitivity of mice, of the swiss random bred strain, previously i.p. vaccinated with bordetella pertussis phase i cells, to histamine was investigated. 2. no response and no increase of the sensitivity of the respiratory system of the pertussis-vaccinated mice was observed during and after the inhaled histamine. 3. no contractions and no response of the isolated perfused ileum of the pertussis-vaccinated mice were observed after the addition of histamine. 4. no experimental or control mo ...1979582991
purification and crystallization of fimbrial hemagglutinin from bordetella pertussis.a procedure to purify and crystallize fimbrial hemagglutinin from bordetella pertussis is described. redissolved crystals had the same specific activity as the original, purified solution of fimbrial hemagglutinin. about 97% of the weight of washed crystals was accounted for by amino acids.197939033
fimbrial hemagglutinin in stationary and shake cultures of bordetella pertussis.bordetella pertussis produced hemagglutinin in stationary cultures; in cultures kept under constant shaking, hemagglutinin was found only during the first 48 h of incubation but not after 3 to 5 days. the type of medium had a pronounced effect on production of hemagglutinin. strain differences in ability to produce hemagglutinin were also detected.197939897
polymorphonuclear leukocyte-inhibitory factor of bordetella pertussis. ii. localization in the outer membrane.the outer and inner membranes and cytoplasm of spheroplasts of a strain of phase i b. pertussis were fractionated by density gradient centrifugation. the high density vesicles of the outer membranes isolated had the "pili" characteristic of the bacteria and the same antigenicty as the bacterial surface. activities for inhibition of polymorphonuclear leukocytes were also almost exclusively localized in this outer membrane fraction. the histamine-sensitizing activity was more dispersed, but its sp ...197943725
immunization against nippostrongylus brasiliensis in the rat. a study on the use of antigen extracted from adult parasites and the parameters which influence the level of protection.it was found that protective immunity in excess of 90% reduction in worm burden could be stimulated against nippostrongylus brasiliensis in rats by using an extract of adult nippostrongylus worms. the level of protection achieved was influenced by several factors. thus, the use of bordetella pertussis as adjuvant significantly increased the level of protection which, in addition, was shown to be influenced by the amount of worm antigen used. furthermore, antigen administered in multiple doses wa ...197944095
non-specific induction of increased resistance in mice to trypanosoma congolense and trypanosoma brucei by immunostimulants.administration of the immunostimulants corynebacterium parvum, bacillus calmette-guérin (bcg) or bordetella pertussis prior to, or at the same time as, challenge with trypanosoma congolense significantly increased survival times in mice, both of trypano-susceptible (a/j) and trypano-resistant (c57bl) strains. the increased survival time was associated with significant alterations in parasitaemia, which included lengthening of the pre-patent period, a delay in the time taken to reach the first pe ...197944360
characteristics of the t-dependent alpha(1 leads to 6) glucosyl (dextran) antibody response induced in mice with isomaltohexaose coupled to chicken gamma-globulin.isomaltohexaose flavazole coupled to chicken gamma-globulin (im6-cgg) induced t cell-dependent anti-alpha(1 leads to 6) dextran-specific igm and igg responses in cba, balb/c and a strain mice. the igg responses were of restricted heterogeneity as judged by isoelectric focusing, and belonged mostly to the igg1 subclass with a minor igg3 component in the case of balb/c and cba mice. all four subclasses of igg were produced in a strain mice. in contrast, native dextran b 512 induces exclusively t c ...197993549
islet-activating protein. enhanced insulin secretion and cyclic amp accumulation in pancreatic islets due to activation of native calcium ionophores.the mechanism whereby "islet-activating protein" (iap) purified from the culture medium of bordetella pertussis potentiates insulin secretion was studied by experiments in vitro with islets of rats once injected with iap (0.5 micrograms/100 g body weight, 3 days before killing) or with islets that had been exposed to iap (0.1 to 100 ng/ml) for 24 h. the iap treatment markedly enhanced insulin secretory responses and camp accumulation in islets, facilitated the efflux of 45ca through the cell mem ...1979216676
corynebacterium parvum as an adjuvant for trypanosoma cruzi epimastigote vaccines: a comparison with saponin and bordetella pertussis.the effect was compared in cba mice of adding corynebacterium parvum, saponin, and bordetella pertussis to living or killed trypanosoma cruzi (y strain) epimastigote vaccines on the induction of protective immunity against subcutaneous (s.c.) challenge with blood trypomastigotes. the addition of c. parvum to a low dose of t. cruzi vaccine, which alone was non-protective, generated a greater degree of protection than did saponin or b. pertussis. c. parvum alone increased resistance to infection t ...1979216967
a protein activator for the adenylate cyclase of bordetella pertussis.the activity of bordetella pertussis extracytoplasmic adenylate cyclase is 100-fold higher in organisms grown on blood agar than in those grown in synthetic medium. this increase in activity is due to in vivo activation of the enzyme by a factor present in erythrocytes. activation also occurs in killed or disrupted organisms. the activator can be separated from heme proteins and has been purified approximately 100-fold from erythrocytes, yielding material of approximately 105,000 daltons. it is ...1979221475
the in vitro effects of bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. iii. b-cell dependence for t-cell proliferation.the nature of the helper lymphocytes in lymphocytosis-promoting factor (lpf)-induced proliferation was explored. removal of macrophages from adherent splenocytes by either carbonyl-iron incubation or passage through sephadex g-10 columns did not affect their synergistic function. nor did cytolysis with thy-1.2 antiserum and complement. the helper cells were found to be surface immunoglobulin-positive (sig+) because they are retained by anti-ig columns, susceptible to lysis by rabbit anti-mouse i ...1979221609
the in vitro effects of bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. iv. generation, characterization, and specificity of cytotoxic lymphocytes.cytotoxic effector lymphocytes were induced in cultures of mouse spleen or lymph node cells by lymphocytosis promoting factor (lpf). the lpf-activated cytotoxic cells: (a) were not generated unless proliferation occurred; (b) sedimented in the lighter density fraction of a bovine serum albumin gradient; (c) were large, blast-like cells; and (d) were lysed by thy-1.2 antiserum plus complement and, therefore, were t cells. neither lpf alone nor supernates from stimulated cultures were cytotoxic. u ...1979221613
evaluation of a ribosomal vaccine against pertussis.a crude ribosomal vaccine derived from bordetella pertussis administered to icr and n:nih (sw) strains of mice protected them effectively against a standardized intracranial challenge. the dose of vaccine that protected half the mice was less for n:nih (sw) than for icr mice and compared favorably with a killed reference vaccine. ribosomes prepared from bacteria ground with washed sea sand were more immunogenic than those obtained by rupture with alumina or with a braun homogenizer. the protecti ...1979222684
the serological diagnosis of whooping cough.indirect haemagglutination (iha), agglutination and complement fixation tests (cft) for bordetella pertussis antibodies were compared on paired sera from 52 suspected cases of whooping cough and single sera from 83 children with no recent history of whooping cough. all three tests detected serotype antibodies 1, 2 and 3, but the iha test was the most sensitive; in seven cases it was the only test to show a rise in titre. it is recommended, particularly with vaccinated children, that the serologi ...1979222838
the influence of different adjuvants on the production of igd and ige antibodies.the aim of this study was to investigate the relationship between the synthesis of igd and ige antibodies upon the administration of antigen. different adjuvants were used in order to find the optimal way of inducing in rats the synthesis of igd and ige antibodies. ovalbumin was used as antigen. fair amounts of ige antibodies were produced using al(oh)3 or bordetella pertussis and al(oh)3 as adjuvant. only small quantities of ige antibodies were formed when either freund's complete adjuvant (fca ...1979219761
[changes in the proliferative response of human lymphocytes in vitro on exposure to subcellular components of bordetella pertussis].different components of b. pertussis were found to have a similar inhibitory effect on thymidine-3h incorporation caused by phytohemagglutinin (pha) in the culture of lymphocytes taken from donors immunized with tetanus toxoid. however, the same fractions of b. pertussis produced differential effects on the reaction of lymphocyte proliferation in response to tetanus toxoid: murein-"ontaining membranes enhanced thymidine-3h incorporation, rna-containing fractions reduced it and water-soluble comp ...1979219921
adjuvant for ige antibody and islet-activating protein in bordetella pertussis.a new purified protein, with a molecular weight of 77,000, isolated from the supernatant of bordetella pertussis culture fluid and called islet-activating protein (iap), had adjuvant activity for ige antibody in addition to leukocytosis-promoting, histamine-sensitizing and islet-activating activities. none of the three subunits (f1, f2 and f3) was biologically active by itself. all of the four activities were recovered in the complexes of subunits f1.3 and f2.3. these results suggest that the ac ...1979220196
intracellular localization of the dermonecrotic toxin of bordetella pertussis.localization of the heat-labile dermonecrotic toxin of bordetella pertussis strain 114 grown in chemically defined stainer-scholte medium was studied by using skin reaction in 4-day-old suckling mice as the assay for toxin. through log phase and into stationary phase of growth the toxin was cell associated and not detected in the culture supernatant. only about 4% of the activity present in a suspension of lysed cells was detected in a suspension of whole cells, and the dermonecrotic activity wa ...1979227787
effects of fatty acids on growth of bordetella pertussis in defined medium.the effects of saturated and unsaturated fatty acids on the growth of bordetella pertussis strain 114 in defined medium were tested. individual fatty acids were found to be either inhibitory or stimulatory to growth, depending on concentration. myristic (c14), pentadecanoic (c15), and palmitic (c16) acids were the most inhibitory saturated fatty acids tested. b. pertussis 114 was extremely sensitive to the unsaturated fatty acids oleic (c18; cis-9), elaidic (c18; trans-9) and petroselinic (c18; ...1979227923
tangential flow filtration of bordetella pertussis submerse cultures.a procedure is reported for the large scale separation of bordetella pertussis microorganisms from liquid culture media by tangential flow filtration (cross flow filtration) using anisotropic membranes with a cut-off limit of 1 x 10(6) daltons, and microporous membranes with a pore size of 0.22 micrometer.1979228969
action of mannitol in various immunological experimental models.the authors describe the inhibiting action of mannitol after repeated administration of low subcutaneous doses in a number of experimental immunological models. for example, in the rat it produces a reduction of the secondary arthritis of freund's adjuvant polyarthritis and also of the pleurisy due to bordetella pertussis hypersensitivity. in the mouse it reduces the reaction of delayed hypersensitivity to sheep red cells. its action is also marked against ovalbumin-induced active skin anaphylax ...1979229721
[serotyping of recently isolated strains of bordetella pertussis. ii. disparity of agglutination titers of patients' sera between antigens prepared from the maeno and recently isolated strains (author's transl)]. 1979231129
effect of fresh guinea-pig serum on the toxicity of bordetella pertussis and bordetella parapertussis. 1979231604
isolation of the lymphocytosis promoting factor-haemagglutinin of bordetella pertussis by affinity chromatography.the lymphocytosis promoting factor-haemagglutinin of bordetella pertussis was isolated from solutions obtained after cell disintegration by a novel affinity chromatographic method using an adsorbent composed of human haptoglobin covalently attached to a sepharose 4b matrix. the haemagglutinin was bound to the adsorbent at ph 6.5 and eluted by a stepwise change to a ph 10 buffer. a 300--600-fold purification of the haemagglutinin was achieved by this one-step process. the chemical and biological ...1979232665
immunological and histological reactions to respiratory administration of ribosomal bordetella pertussis vaccine in mice. 1979232799
action of various drugs with anti-inflammatory or anti-rheumatic properties on pleurisy due to bordetella pertussis hypersensitivity in the rat.the authors studied various drugs with anti-inflammatory or antirheumatic properties on pleurisy due to bordetella pertussis hypersensitivity in the rat. the following compounds were studies according to various methods of treatment: indomethacin, phenylbutazone, cyclophosphamide, desonide, chloroquine, levamisole, d-penicillamine and sodium aurothiopropanol sulphonate. the action on the volume of pleural exudate and on cell events depended on the compounds and the conditions of treatment. only ...1979232922
pertussis toxin: the cause of the harmful effects and prolonged immunity of whooping cough. a hypothesis.the nature of the pathogenesis and of the prolonged immunity of whooping cough has not been clearly defined. the literature of bordetella pertussis indicated that only the antigen that induces histamine sensitization, lymphocytosis, and other biological reactions in mice is the cause of the harmful effects and prolonged immunity of whooping cough. this antigen has the general characteristics of bacterial protein exotoxins that cause the harmful effects of infectious diseases such as diphtheria a ...1979233166
antigens in penicillin allergy. iii. antigen and antibody levels in mice treated with pure and contaminated penicillins.using a radioimmunoassay, it was shown that commercially available ampicillin preparations often contain penicilloylated high molecular weight impurities. these possess immunological activities and stimulate penicilloyl-specific antibody formation in mice treated according to a therapeutic schedule. using purified and experimentally contaminated preparations it was also found that exposure of the animals to escherichia coli and bordetella pertussis bacteria could increase the antibody formation ...1979217840
experimental studies on the biological properties of bordetella pertussis. 1979262507
comparison of vaccination of mice and rats with haemophilus influenzae and bordetella pertussis as models of atopy.1. rats and mice were vaccinated with haemophilus influenzae in different vaccination schedules whereafter blood eosinophils were counted. in rats a single vaccination resulted in a dose-dependent effect on the blood eosinophil count in a pattern comparable with that after bordetella pertussis vaccination. in a long-term vaccination schedule (five times a week for 5 weeks) rats developed a constant eosinophilia. in mice a single vaccination resulted in an eosinopenia of a consistent pattern whic ...1979311260
a method for the induction of a prolonged elevated igm response without the formation of igg antibody, by injecting carrageenan-treated mice with multiple doses of sheep erythrocytes.carrageenan, a known macrophage toxin and immunosuppressive agent, has been studied for its effect on antibody responses following single doses or multiple daily increasing doses of sheep red blood cells (srbc) injected intraperitoneally (i.p.) in male cba strain mice. results showed that lambda carrageenan administered prior to a single i.p. injection of srbc markedly suppressed both the igm and igg responses. however, when carrageenan-treated mice were injected with multiple daily increasing a ...1979313964
host immune responses after administration of inactivated venezuelan equine encephalomyelitis virus vaccines--iii. kinetics for neutralizing antibody immunoglobulin class responses in donors and adoptively-immunized recipients.c57b16 mice were immunized with either live, attenuated tc-83 strain vee virus vaccine or formalin-inactivated vee vaccine combined with bordetella pertussis. the kinetics of specific donor and adoptively-immunized recipient anti-vee neutralizing antibody responses were studied. donor mice immunized with either live or inactivated vee virus vaccine combined with potent adjuvants develop specific anti-vee igm and igg responses as early as 7 days post-immunization. anti-vee igm antibody responses ...1979318539
effect of in vivo pretreatment of rats with a new protein purified from bordetella pertussis on in vitro secretion of insulin: role of calcium.rats were injected once iv with an islet-activating protein (iap), a new protein purified from the culture medium of bordetella pertussis. three days later, their pancreases were studied in vitro for insulin secretory responses. as with pertussis vaccine, pretreatment of rats with iap was effective in enhancing insulin release from pancreas during perfusion or from islets during incubation in response to secretagogues such as glucose and glibenclamide. the alpha-adrenergic inhibition of insulin ...1979376293
the deleterious effect of bordetella pertussis vaccine and poly(ri.rc) on the metabolism and disposition of phenytoin.the elimination of phenytoin from blood was significantly decreased in rats pretreated 24 hr previously with bordetella pertussis vaccine or the synthetic polynucleotide poly(ri.rc.). phenytoin half-life was increased about 4-fold by both agents. in microsomes prepared from rats pretreated with b. pertussis vaccine or poly(ri.rc) the hydroxylation of phenytoin in vitro was decreased by 36 nad 53%, respectively. the addition of these agents to normal microsomal suspensions had no effect on phenyt ...1979762660
allergenicity of concanavalin a in mice.concanavalin a (con a) is a potent allergen in certain strains of mice and in particularly the h-2kk mice, a/j, cba/h, and c3h/he. using a dose of 100 microgram, the subcutaneous route of injection was the most effective means of inducing high, persistent titers of t cell-dependent circulating anti-con a reagins without the addition of the classical ige adjuvants, aluminium hydroxide and bordetella pertussis vaccine. haptenated con a induced reagins with antihapten specificity. in the discussion ...1979437908
influence of mode of blood sampling on the immunoreactive insulin concentration in serum of bordetella pertussis-treated mice [proceedings]. 1979454932
[counterimmunoelectrophoresis in the diagnosis of bordetella pertussis].the serum of 50 patients between 25 days to 15 years old who were admitted with the diagnosis of pertussis syndrome were investigated for precipitating antibodies by means of discontinuous counterimmunolectrophoresis (cied). the presence of antibodies for bordetella pertussis antigen was shown in 28 cases. all samples were taken within the first 24/48 hours of admission and at convalescence. the technique is easy to carry out, quick and of low cost. this test offers an adequate and fast means to ...1979486252
comparative antitumor effects of corynebacterium parvum, bordetella pertussis, bacillus calmette-guérin, and levamisole alone or in combination with cyclophosphamide in the cad2 murine mammary adenocarcinoma system.the antitumor efficacy of various immune stimulants [corynebacterium parvum, bordetella pertussis, and bacillus calmette-guérin (bcg)] and levamisole alone or in conjunction with cyclophosphamide (cy) was studied in the cad2 mammary adenocarcinoma system using schedules developed previously with c. parvum and cy. weekly systemic treatment with c. parvum, b. pertussis, or bcg was effective in controlling tumor growth and had equivalent antitumor effects, but weekly treatment (or a single treatmen ...1979498111
loss of adjuvanticity in rats for the hyperacute form of allergic encephalomyelitis and for reaginic antibody production in mice of a phenotypic variant of bordetella pertussis.the adjuvanticity of a phenotypic (c-mode) variant of b. pertussis, known to be deficient in certain immunological and physiopathological properties, was compared to that of the normal (x-mode) strain. the x-mode vaccine was a potent adjuvant for induction of hyperacute experimental allergic encephalomyelitis to guinea-pig spinal cord in lewis rats whereas c-mode vaccine was inactive. x-mode vaccine was also highly active in the induction of reaginic (both ige and iggl) antibodies to ovalbumin i ...1979500113
effect of ascaris suum and other adjuvants on the potentiation of the ige response in guinea-pigs.the potentiation effect of various adjuvants on the production of guinea-pig ige was investigated using freund's complete and incomplete adjuvant, the lipopolysaccharide of escherichia coli and salmonella typhosa, bordetella pertussis, and the nematodes nippostrongylus brasiliensis and ascaris suum. while all the antigens had a variable effect on the potential of the igg response, only infection with a. suum resulted in an enhanced ige response to the antigen, egg albumin. maximum potentiation o ...1979500118
effect of bordetella pertussis vaccine on the course of lymphocytic choriomeningitis (lcm) virus infection in suckling mice pretreated with dianhydrodulcitol (dad).in earlier experiments bordetella pertussis vaccine was found to enhance and dianhydrodulcitol (dad) to weaken cellular immune response to intracerebral lcm virus infection in suckling mice. b. pertussis vaccine proved to inhibit the restrictive effect of dad produced on the immune response in mice when 2-to-4-days-old animals were pretreated with dad and subsequently, at the age of 16 to 18 days of life, treated with b. pertussis vaccine then infected with lcm virus. consequently, b. pertussis ...1979539467
comparison of the effect of phenylbutazone, desonide and cyclophosphamide on four types of experimental pleurisy.the action of phenylbutazone, a non-steroid anti-inflammatory drug, desonide, a corticosteroid, and cyclophosphamide, an immunosuppressant agent, was studied on four types of experimental pleurisy: carrageenan-pleurisy in rats; passive reversed arthus pleurisy in rats; bordetella pertussis-delayed hypersensitivity pleurisy in rats and ppd (purified protein derivative)--delayed hypersensitivity pleurisy in guinea-pigs. for each compound, the action on the exudate and on the number of the differen ...19806106673
improvement of diabetic symptoms of hereditary diabetic (kk) mice by a single injection with islet-activating protein (iap).islet-activating protein (iap) prepared from the culture medium of bordetella pertussis cells was very effective in eliminating diabetic symptoms characteristic of hereditary diabetic kk mice over a long period of time. after a single injection of iap (5 microgram/kg body wt) into kk mice, the non-fasting concentration of blood glucose was maintained nearly normal over 2 weeks, with a gradual return to the pre-iap level 30 days later. during this period, glucose tolerance was normalized and only ...19806257880
polymorphonuclear leukocyte-inhibitory factor of bordetella pertussis. iii. inhibition of arthus reaction and peritoneal infiltration of pmn.in vivo biologic effects of the polymorphonuclear leukocyte-inhibitory factor (pif) of bordetella pertussis were tested by using two experimentally induced inflammatory processes in mice. the intravenous injection of a partially purified extract from phase i bacteria strongly inhibited the glycogen-induced peritoneal infiltration of polymorphonuclear leukocytes (pmn) and the arthus reactions, whereas little inhibitory activity was found in the extract from phase iii bacteria. the activity was lo ...19806258031
[treatment of pertussis with cefoperazone (author's transl)].clinical trial of cefoperazone (cpz) for the treatment of 15 cases suffering from pertussis was performed and the results were as follows. the method of administration, as a rule intravenous infusion was performed at 100 approximately 200 mg/kg/day. (1) seventeen strains of bordetella pertussis showed under 0.006 mcg/ml of mic. it was similar to pipc. (2) any effect to hepatic or renal function was not observed. (3) clinical response obtained in these cases was excellent in 13 cases (86.7%), goo ...19806261023
in vitro assay for histamine-sensitizing factor of bordetella pertussis. 19806261097
[dynamics of homocytotropic antibody production in pigs].homocytotropic antibody (hta) production in pigs was stimulated by sensitization with alum precipitated equine serum or with equine serum in freund's complete adjuvant. two doses of appropriate antigen were given in 7 days interval simultaneously with heat inactivated suspension of bordetella pertussis organisms. hta were detected in 6 of 10 pigs sensitized with alum precipitated serum and in 4 of 6 pigs sensitized with serum in freund's complete adjuvant. in pigs sensitized with alum precipitat ...19806259632
[treatment of whooping cough and whooping cough syndrome by cefoperazone (author's transl)].clinical trial of cefoperazone (cpz) for the treatment of whooping cough and whooping cough syndrome was performed and the following results were obtained. 1) in 18 cases suffering from whooping cough, cpz was given 47 approximately 106 mg/kg/day (average 72 mg/kg/day) by intravenous route. the clinical efficacy rates judged by doctors in charge were 44% on the 3 rd day, 75% on the 7 th day, 86% on the period day. and in these cases, the clinical efficacy rates judged by committee members were 5 ...19806264178
morphological alterations of the adrenal gland following administration of histamine-sensitizing-factor of bordetella pertussis. 19806267538
failure of synthetic muramyl dipeptide to increase antibacterial resistance.synthetic muranyl dipeptide, which potentiates antibody production and cellular immune responses at a dosage of 100 to 500 micrograms, did not enhance resistance to intravenous infection with a sublethal dose of 2 x 10(3) to 4 x 10(3) viable listeria monocytogenes cells in mice when intraperitoneally injected either 20 min or 5 days before infection. similarly, blockade of the mononuclear phagocyte system by dextran sulfate 500 could not be overcome by pretreatment with muramyl dipeptide. in con ...19806155329
ige antibodies produced in mice instrumental in analyses of antigenicity of cephalothin preparation.ige antibodies to a cephalothin preparation were produced in mice which had been immunized with cephalothin-ascaris extract conjugate mixed with al(oh)3 gel plus bordetella pertussis. mice which had been irradiated just before receiving the booster injection wtih the conjugate showed higher ige antibody levels against the preparation in comparison with unirradiated mice. the specificities of ige antibodies against the preparation were examined with an inhibition test of passive cutaneous anaphyl ...19806158486
hapten antibody production and the relevance of allogeneic reactions to elimination of the carrier effect.lewis (lew) rats immunized 3 weeks before by injection of dnp-klh together with bordetella pertussis showed high levels of dnp antibody as judged by serum binding of 10(-7) m 3h-dnp-lysine 10 days after secondary immunization with dnp-klh. sera obtained from lew rats following secondary immunization with dnp-bgg showed reduced dnp hapten binding. however, injection of 10(8) f1 hybrid lewis x brown norway spleen cells into dnp-primed lew rats 2 days before secondary immunization with dnp-bgg sign ...19806159332
[method of preserving the biological properties of and transporting bordetella pertussis]. 19806163897
preservation of nasopharyngeal smears for fluorescent antibody detection of bordetella pertussis.smears from throat and nasal washings seeded with bordetella pertussis were either treated with aprotinin (a protease inhibitor) or fixed with 1 or 10% formalin. these smears were stored at 23, 4, and -70 degrees c. smears were removed and stained with a fluorescent antibody conjugate for b. pertussis at intervals during 22 days of storage. results indicate that treatment of smears with 4 or 8 u of aprotinin per ml preserved fluorescent antibody staining qualities of b. pertussis for 22 days; fi ...19806171579
the in vitro effects of bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. v. modulation of t cell proliferation by helper and suppressor lymphocytes. 19806243149
reactions in the foot pads of mice induced by pertussigen and endotoxin from bordetella pertussis. 19806245208
biological activities of fragments derived from bordetella pertussis endotoxin: isolation of a nontoxic, shwartzman-negative lipid a possessing high adjuvant properties.endotoxin from fresly sedimented bordetella pertussis cells, isolated by the phenol/water procedure when submitted to kinetically controlled, mild acidic hydrolysis released a polysaccharide (polysaccharide 1), a complex lipid (lipid x), and a glycolipid. when treated with somewhat stronger acid, the glycolipid yielded a second polysaccharide (polysaccharide 2) and another complex lipid (lipid a). the intact pertussis endotoxin had all the usual properties of endotoxins extracted from enteric ba ...19806247278
adenylate cyclase activity of bordetella organisms. i. its production in liquid medium.abundant adenylate cyclase activity was found in the phase i cultures not only of bordetella pertussis but also fo b. parapertussis and b. bronchiseptica. the enzyme activity in the culture fluid increased rapidly and reached a peak during the logarithmic growth phase. b. parapertussis and b. bronchiseptica especially produced a high activity of the enzyme in the culture fluid during the logarithmic phase, but little or no activity was detected in the cells throughout the growth period. in the c ...19806247626
effects of islet-activating protein (iap) on blood glucose and plasma insulin in healthy volunteers (phase 1 studies).islet-activating protein (iap) is a new active substance purified from the culture medium of bordetella pertussis. the active protein possesses a molecular weight of 77,000 and an isoelectric point of ph 7.8. the nature of iap-action is characterized by enhancement of insulin secretory response to glucose and other stimulants. a single injection of iap into spontaneous diabetes rats resulted in normalization of their glucose intolerance over a period of a month. acute and chronic animal toxicity ...19806247781
adjuvants in the induction and enhancement of rat ige responses.in hooded lister rats the primary ige antibody response induced by immunization with antigen and adjuvant may be enhanced either specifically by a further dose of antigen (booster response) or non-specifically by infection with helminth parasites (potentiated response). the initial immunizing technique can influence the occurrence and level of these enhanced responses and here we describe the effect of using different adjuvants in the priming event. although the level of the primary response was ...19806248278
the comparative selectivity of adjuvants for humoral and cell-mediated immunity. i. effect on the antibody response to bovine serum albumin and sheep red blood cells of freund's incomplete and complete adjuvants, alhydrogel, corynebacterium parvum, bordetella pertussis, muramyl dipeptide and saponin.a comparison was made of seven recognized adjuvants, freund's incomplete and complete, alhydrogel, corynebacterium parvum, bordetella pertussis, muramyl dipeptide and saponin, administered with bsa or srbc by the s.c. route of immunization. strong selectivity as well as differences in potency were revealed in relation to these two antigens. only fia, fca, alhydrogel and mdp promoted the primary response to 50 microgram of bsa, and fia was significantly superior to fca. immunological memory to a ...19806248282
the comparative selectivity of adjuvants for humoral and cell-mediated immunity. ii. effect on delayed-type hypersensitivity in the mouse and guinea pig, and cell-mediated immunity to tumour antigens in the mouse of freund's incomplete and complete adjuvants, alhydrogel, corynebacterium parvum, bordetella pertussis, muramyl dipeptide and saponin.corynebacterium parvum was the only adjuvant of those tested which consistently potentiated delayed-type hypersensitivity (dth) to sheep red blood cells (srbc) in the mouse, although this required the antigen-adjuvant mixture to be injected subcutaneously in the footpad rather than the flank. for induction of dth to ovalbumin (ova) in the guinea pig, c. parvum and mdp could be substituted for the mycobacteria in fca. c. parvum was effective in aqueous solution provided that the ova was absorbed ...19806248283
involvement of a heat-stable and heat-labile component of bordetella pertussis in the depression of the murine hepatic mixed-function oxidase system. 19806249317
variability in derivative strains of bordetella pertussis.bordetella pertussis strain ut25 was isolated on bordet-gengou(b-g) agar from a child with whooping cough in 1977. derivative strains were isolated from ut25 by serial passages on trypticase soy agar (tsa) containing blood,followed by serial passages on plain tsa. at passage 69, ut25 was subcultured in parallel on tsa and b-g. for experiments, strains were grown in defined liquid medium, adjusted to a standard turbidity, and tested in parallel. tests were performed to detect changes in immunogen ...19806249689
aerosol infection of mice with bordetella pertussis.aerosol inhalation of bordetella pertussis tohama phase i resulted in a reproducible and uniform infection of mice (strain ddy or icr). mice in groups of 10 exposed for 30 min to aerosols generated from bacterial suspensions of 10(9) and 10(10) organisms per ml resulted in mean bacterial counts of 2.3 (+/- 0.3) x 10(4) and 1.0 (+/- 0.3) x 10(5) colony-forming units, respectively, in the lung of each animal. subsequent studies using a 30-min aerosol inoculation of icr mice with 2 x 10(9) bacteria ...19806249758
isolation of two protein-free and chemically different lipopolysaccharides from bordetella pertussis phenol-extracted endotoxin.endotoxin prepared from several bordetella pertussis strains in both immunological phases i and iv gave two lipopolysaccharide peaks (lps-i and lps-ii) when analyzed on hydroxylapatite columns in a phosphate buffer containing 0.1% sodium dodecyl sulfate; these lipopolysaccharides, present in the ratio of 2:3, are true endotoxins by both chemical and biological criteria. endotoxins isolated from escherichia coli, salmonella typhimurium, and shigella flexneri gave single lipopolysaccharide peaks w ...19806249793
bordetella pertussis respiratory tract infection in the mouse: pathophysiological responses.the influence of living bordetella pertussis on the induction and duration of pathophysiological reactions in mice infected intranasally with graded doses of culture was studied. lethally infected mice showed loss of body weight, spleen atrophy, pronounced hypothermia and hypoglycemia, and highly elevated levels of leukocytes and serum immunoreactive insulin. sublethally infected mice showed normal weight gain, practically normal temperature, spleen enlargement, lesser pronounced hypoglycemia, l ...19806249873
etiology of pertussis syndrome.one hundred patients with clinical pertussis were studied to determine the etiology of pertussis syndrome. forty-two (42%) of the patients had either bordetella pertussis of bordetella parapertussis isolated from the nasopharynx. in additional 36 (36%) patients, b pertussis was isolated from the nasopharynx of the associated index case or family contact case. thus, bordetella was isolated from 78 (78%) of the patients or from their immediate family group. of the 22 culture-negative patients resi ...19806250124
injury of respiratory epithelium.the large surface area provided by the respiratory tract epithelium of humans for exposure to microbial agents and toxic substances in the environment makes this organ system very vulnerable but a good early indicator of adverse health effects. however, the complexity of pulmonary defense mechanisms complicates definition of the interactive effects of pollutants and infectious agents. tracheal organ culture has been utilized to maintain organized, differentiated respiratory epithelium in vitro. ...19806250814
[bordetella pertussis bacteriophage].for the first time bordetella pertussis bacteriophage was isolated, and its presence was confirmed by electron microscopy and by agar layer titration. the lysogenic strains were activated by their treatment with mitomycin c in a dose of 4.5 mg/ml. the phage system of the bordetella genus, heretofore unknown, has been revealed: bordetella pertussis phage lyzed all the tested strains of bordetella parapertussis (25 strains) and could be passaged in these strains. the phage formed turbid and transp ...19806251680
antibiotic susceptibility testing of bordetella pertussis.a tube dilution test to evaluate the effectiveness of antibiotics against bordetella pertussis is described. five b. pertussis strains, including a well-characterized research strain and four fresh clinical isolates, were tested with several antibiotics. erythromycin showed the highest in-vitro activity of the antibiotics tested. a concentration of 0.12 microgram/ml was bacteriostatic for all strains, while 2 microgram/ml was bactericidal. minimal inhibitory and minimal bactericidal concentratio ...19806251713
inhibition of hypersensitivity reactions by 16(s)-methyl-20-methoxy-pge2 (ypg-209) in animals.the effects of 16(s)-methyl-20-methoxy-pge2 (ypg-209) on hypersensitivity reactions were investigated in the rat, guinea-pig and mouse. intravenously administered ypg-209 was 150 times as potent as disodium cromoglycate (dscg) in the inhibitory effect on the ige(mouse)-mediated 24 hr rat pca. when administered intravenously to guinea-pigs, ypg-209 inhibited significantly the ige (guinea-pig)-mediated 8 day guinea-pig pca, whereas dscg exhibited no significant inhibitory effects. the oral doses o ...19806251764
the isolation of a restriction enzyme from bordetella pertussis. 19806251833
slow interaction of islet-activating protein with pancreatic islets during primary culture to cause reversal of alpha-adrenergic inhibition of insulin secretion.the manner in which islet-activating protein (iap), a protein purified from the culture medium of bordetella pertussis, interacts with the islet b-cell was studied by following the progressive development of iap-induced reversal of alpha-adrenergic inhibition of insulin release during maintenance of islets in culture with glucose and epinephrine. this action of iap developed in an exponential manner dependent on its concentration after a true lag period of about 1 h. the lag period was not gross ...19806253447
calmodulin activates prokaryotic adenylate cyclase.the adenylate cyclase of bordetella pertussis is stimulated 100- to 1000-fold in a dose-dependent manner by calf brain calmodulin. the system has the following properties. (i) the activation is prevented by ethylene glycol bis(beta-aminoethyl ether)-n,n,n',n'-tetraacetic acid and restored by ca2+. (ii) oxidation of the methionine residues of calmodulin abolishes the ability to activate the cyclase. (iii) trifluoperazine inhibits calmodulin-activated cyclase. (iv) a troponin c preparation stimula ...19806253992
[aerosol vaccination of mice with inactivated influenza virus (author's transl)].nmri mice were vaccinated by the aerosol technique, using the ethylethylenimine inactivated and polyethylenglycol concentrated virus strain a/pr/8/34 (ho/n1) with or without addition of bordetella pertussis extract (bpe) as an adjuvant. the immune response of the vaccinated animals was controlled by challenge infection via aerosol technique and by examination of hai antibodies in the serum and in the washings of lungs. after a single aerosol vaccination a weak protection was observed only, if th ...19806254287
effect of bordetella pertussis vaccination in mice and the isolated tracheal response to isoprenaline.the administration of bordetella pertussis vaccine to mice has been associated with the development of an impaired beta-adrenoceptor responsiveness and in many respects has resembled human asthma. trachea (n = 12) were isolated from swiss-webster mice 5 days following the intraperitoneal administration of 2 x 10(9) b. pertussis organisms. the tracheal smooth muscle response to carbachol was measured and compared with that found in trachea from unvaccinated mice (n = 15). the contractile response ...19806255813
passive paw anaphylaxis in the rat. optimum conditions for use in studies on immediate hypersensitivity.the difficulties involved in quantitating passive cutaneous anaphylaxis led us to examine the rat paw as a site for passive anaphylaxis and to define optimum conditions for passive paw anaphylaxis. generation of homocytotropic antibodies in a number of different rat strains revealed that female brown norway rats were excellent producers of high titre antisera after only a single injection of antigen and bordetella pertussis. injection of ratpaws with diluted antisera followed by short (1-2 h) or ...19806255814
the lymphoid leukocytoses.the experienced morphologist can be extremely helpful to the clinician by virtue of his or her ability to distinguish among the various subtypes of reactive lymphocytoses. an awareness on the part of the clinician as to the nuances of subclassification may lead to earlier diagnosis of a disease process. broadly, proliferations of normal lymphocytes point to infectious lymphocytosis or bordetella pertussis infection. proliferations of atypical lymphocytes, especially when minimum diagnostic crite ...19807352124
immunotherapy of postoperative metastases of 13762a rat mammary adenocarcinoma: comparative effectiveness of bcg substrains and methods of preparation.six bacillus calmette-guérin (bcg) substrains (phipps, pasteur, connaught, glaxo, riv, and tice) and two methods of preparation and storage (pellicle-lyophilized, dispersed-frozen and dispersed lyophilized) were directly compared in a standard immunotherapy protocol. complete freund's adjuvant and bordetella pertussis vaccine were also studied. all experiments included direct comparisons with corynebacterium parvum. the immunotherapy assay system was the 13762a rat mammary carcinoma in which imm ...19807397623
neuropathology of experimental allergic encephalomyelitis in inbred strains of mice.ten inbred strains of mice were tested for their susceptibility to experimental allergic encephalomyelitis (eae) after sensitization with mouse spinal cord in complete freund's adjuvant followed by booster injections of bordetella pertussis. the results extended previous findings in that not all susceptible mice possessed the h-2s haplotype, but mice with an h-2q background (dba1/j strain) were also susceptible. neuropathologic examination of experimental allergic encephalomyelitis in the mouse ...19807401630
reduced tracheal mast cell numbers in adjuvant-sensitized rats associated with reduced pulmonary reactivity.pvg/c inbred rats were sensitized by intra-peritoneal injection of dnp 19-ovalbumin with or without heat-killed bordetella pertussis as adjuvant. the use of adjuvant was associated with a greater serum ige, greater cutaneous immediate hypersensitivity, but no increase in immediate pulmonary reactivity to aerosol challenge. the number of mast cells in the trachea of adjuvant-sensitized rats was significantly reduced when compared with both unsensitized and allergen sensitized rats but this reduct ...19807429532
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