Publications

reverse transcription and polymerase chain reaction amplification of rrna for detection of helicobacter species.	sequence data on helicobacter pylori 16s rrna were used to select two 22-base oligonucleotide primers for use in a polymerase chain reaction (pcr) for detection of h. pylori. h. pylori cells were treated with lysis buffer, boiled, and chloroform extracted. reverse transcription of rrna was followed by pcr amplification (rt-pcr) of the synthesized cdna and 16s rrna gene. the amplified pcr products were analyzed by agarose gel electrophoresis and southern blotting. using ethidium bromide-stained a ...	1992	1383268
vitronectin binding by helicobacter pylori.	vitronectin, a serum and extracellular matrix protein involved in immunological reactions, interacts with helicobacter pylori strains. of the 20 h. pylori strains tested three strains bound more than 50% of the vitronectin added, five strains bound 25-40%, nine strains bound 10-20% and three strains bound 5-8% vitronectin. two strains, one with high- and one with low-binding properties, were selected for further characterization of 125i-vitronectin binding. binding to the urea-activated 125i-lab ...	1992	1384604
inhibition of gastric mucosal laminin receptor by helicobacter pylori lipopolysaccharide: effect of ebrotidine.	the effect of lipopolysaccharide from h. pylori, a bacteria implicated in the etiology of gastric disease, on the gastric mucosal laminin-receptor interaction was investigated. the receptor protein, prepared from rat gastric epithelial cell membrane by affinity chromatography on laminin-coupled sepharose, was radioiodinated, and incorporated into liposomes which exhibited specific affinity towards laminin-coated surface. the binding was inhibited by h. pylori lipopolysaccharide, which caused a m ...	1992	1385705
refractory peptic ulcers.	ulcers that do not heal after 8 weeks of treatment with standard-dose regimens of antiulcer drugs are considered refractory. incidences of duodenal and gastric ulcers refractory to h2-receptor antagonists are 9% and 20%, respectively. when treated with a single daily dose of omeprazole 20 mg, duodenal ulcers have a refractory incidence of 3% and gastric ulcers, 11%. omeprazole's benefit may result from its potent gastric antisecretory action. acid hypersecretion is an important pathophysiologic ...	1992	1386767
prevalence of antibodies against heat-stable antigens from helicobacter pylori in patients with dyspeptic symptoms and normal persons.	heat-stable antigens from helicobacter pylori were investigated for the detection of serum igg, iga and igm antibodies against h. pylori by an elisa technique. antibody titers against h. pylori were measured in 167 dyspeptic patients, of whom 96 were h. pylori positive confirmed by culture or microscopy, and in 482 controls (0-98 years). increased igg antibody titers were found significantly more often in dyspeptic patients with active chronic gastritis than in patients with normal morphology, a ...	1992	1389098
[use of pcr in the epidemiological identification of campylobacter spp].	with arbitrary primer pcr technique it is possible to obtain amplification lane patterns easily and with good reproducibility from the genomic dna of bacteria studied. there is also no need for prior information regarding the dna sequence.	1992	1391017
[helicobacter pylori].		1992	1391020
treatment of peptic ulcer disease with furazolidone.	furazolidone (fz) has been used in china as a treatment of peptic ulcer disease for about 20 years. clinical and experimental studies suggest that it has good short-term and long-term effects on both human and animal ulcers. the ulcer healing rate is related to the dosage and course of treatment. the healing rate of a high dose, 2 week course is about 70-75% and the relapse rate after 3 years is 9.5%. the adverse reactions to fz are not severe, and are well tolerated in most patients. however th ...	1992	1391736
preliminary observations in the fasting serum gastrin in patients with duodenal ulcer; further evidence of the "clearing" effect of omeprazole on h pylori?	thirty patients with active duodenal ulcer who were helicobacter pylori positive (hp+) by hlo test and by histology (giemsa stain) were given omeprazole (ome) 20 mg/d for a two-week period. estimation of fasting serum gastrin concentration (ria) was performed before treatment and 24 hours after the last dosage of ome, and hp was searched for an antral biopsies at the end of the treatment as well. mean fasting serum gastrin concentration increased significantly after treatment in all patients stu ...	1992	1392017
the association of helicobacter pylori infection with low levels of urea and ph in the gastric juices.	fifty-four gastric biopsies and their relative gastric juices were analyzed for the presence of helicobacter pylori with both cultural and microscopic methods. thirty-one samples were positive and twenty-three were negative. these data were therefore employed as references for the subsequent comparisons. furthermore, the gastric juices were later tested to establish the urea concentration and the ph level. in addition, the sediment obtained after centrifugation was microscopically observed for t ...	1992	1392020
double blind trial of colloidal bismuth subcitrate versus placebo in helicobacter pylori positive patients with non-ulcer dyspepsia.	we have carried out a double blind placebo controlled trial to assess the effects of treatment with colloidal bismuth subcitrate in helicobacter pylori associated non-ulcer dyspepsia. eighty patients with dyspepsia, normal upper gastrointestinal appearances at endoscopy and h pylori associated active chronic gastritis on histology of gastric antral biopsies were included in the trial. the patients were randomised to receive colloidal bismuth subcitrate 240mg twice daily for four weeks or matchin ...	1992	1392023
helicobacter pylori: a nihilistic perspective.		1992	1392405
maintenance therapy for peptic ulcer disease: an overview of medical treatment.	peptic ulcer disease is a chronic disorder that manifests as recurrent episodes of pain or complications such as bleeding or perforation. current medical therapy with h2-receptor blockers or sucralfate effectively prevents most recurrences and, according to clinical trials, may prevent complications. since this treatment does not alter the natural history of peptic ulcer disease, continued therapy is needed. surgical treatment does permanently alter the history of recurrence. medical therapy aim ...	1992	1392406
randomised controlled trial of short term treatment to eradicate helicobacter pylori in patients with duodenal ulcer.	to determine whether one week's drug treatment is sufficient to eradicate helicobacter pylori in patients with duodenal ulcer.	1992	1392995
gas phase derivatization of ammonia with 4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole and its application to urease assay.	an ammonia-specific and rapid fluorometric method for determination of ammonia and urease activity was developed. the method is designed to assay ammonia levels or urease activity for the rapid diagnosis of helicobacter pylori infection. 4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole was used to derivatize ammonia and 4-amino-7-nitrobenzo-2-oxa-1,3-diazole was analysed by high performance liquid chromatography at an excitation wavelength of 455 nm and an emission wavelength of 520 nm. derivatization wa ...	1992	1394655
[the advances in immunological research of helicobacter pylori].		1992	1395917
significance and regulation of gastric secretion of platelet-activating factor (paf-acether) in man.	platelet-activating factor (paf) has been implicated in the pathogenesis of acute inflammatory and ulcerative diseases of the upper gastrointestinal tract. in the present study, we compared the gastric output of paf and its precursors with gastric acid output, in patients with various upper gastrointestinal tract diseases and healthy controls. paf and precursors were also extracted from gastric biopsies from subjects with chronic gastritis and/or gastric colonization by helicobacter pylori. unde ...	1992	1396006
use of a urea breath test versus invasive methods to determine the prevalence of helicobacter pylori in zaire.	the prevalence of helicobacter pylori infection in zaire was determined by means of a [14c] urea breath test in 133 asymptomatic subjects, by culture and histological examination of biopsies in 324 consecutive endoscopy patients with chronic epigastric complaints, and by both the breath test and culture/histology in a subset of 92 patients. sixty healthy belgian students or hospital laboratory workers were also included for comparison. the prevalence of helicobacter pylori was significantly high ...	1992	1396751
an assessment of serological tests for detection of helicobacter pylori.		1992	1396763
effect of antimicrobial therapy on the specific serological response to helicobacter pylori infection.	the systemic immune response to helicobacter pylori was studied in 247 infected adult patients before antimicrobial therapy and at different intervals following therapy. endoscopy with simultaneous collection of biopsies was performed in all patients immediately before treatment, 4 to 6 weeks after the end of therapy and 6 to 12 months later. a 14c-urea breath test was performed 3 to 6 months after the end of treatment. biopsy specimens were cultured and examined histologically using giemsa stai ...	1992	1396764
serodiagnosis of helicobacter pylori infections by detection of immunoglobulin g antibodies using an immunoblot technique and enzyme immunoassay.	a transferable solid phase enzyme immunoassay (tsp-eia) and an immunoblot technique were evaluated for the detection of igg antibodies against helicobacter pylori. using the biopsy urease test as reference method, the sensitivity and specificity of the eia were 96% and 100%, respectively. immunoblot analysis was carried out by testing sera from patients with a positive urease test who suffered from type b gastritis, gastric and duodenal ulcers, and a negative control group. the immunoblotted hel ...	1992	1396765
serodiagnosis of helicobacter pylori infections with an enzyme immunoassay using the chromatographically purified 120 kilodalton protein.	a membrane-associated 120 kda protein of helicobacter pylori with known species-specificity was isolated and used in an enzyme immunoassay (eia) for the detection of helicobacter pylori-specific igg antibodies in patient sera. the eia was compared with two other methods used for serodiagnosis of helicobacter pylori infections: an eia using sonicated whole helicobacter pylori cell antigen and western immunoblot. in a prospective study 127 unselected patients (76 patients with antrum gastritis, 51 ...	1992	1396766
evaluation of two commercial enzyme immunoassays for the diagnosis of helicobacter pylori infection.	sera from 65 patients with upper gastrointestinal tract symptoms and proven helicobacter pylori infection, and from 42 negative controls were tested with two commercial eias (gap test, biorad; and ecp test, biometra) and two non-commercial eias, one performed with whole sonicated cells and the other with acid extract of helicobacter pylori as antigen. the gap assay showed a sensitivity of 83.1% and a specificity of 47.6%. the ecp assay showed a sensitivity of 87.7% and a specificity of 61.9%. fo ...	1992	1396775
helicobacter pylori, peptic ulcer disease and inhibition of gastric acid secretion.	recent studies have been reviewed to establish the possible importance of the interaction between helicobacter pylori infection and gastric acid secretion. h. pylori infection results in increased gastrin release, but this does not lead to gastric acid hypersecretion and gastrin normalizes after eradication of the infection. an optimal, well-tolerated treatment strategy against h. pylori infection has not yet been clearly defined. one potentially useful approach may be to improve the antibacteri ...	1992	1397740
natural history of gastritis and its relationship to peptic ulcer disease.	chronic gastritis is a common inflammatory disease. in a number of patients, the inflamed gastric mucosa shows a gradual tendency to become atrophic (atrophic gastritis). gastritis tends to be lifelong, and spontaneous healing is rare. with very few exceptions (e.g. in patients with autoimmune chronic corpus gastritis), gastritis is associated with the presence of the bacterium helicobacter pylori. inflammation and atrophy of the gastric mucosa result in impairment of gastric secretory functions ...	1992	1397747
helicobacter pylori secretes a chemotactic factor for monocytes and neutrophils.	helicobacter pylori is associated with an inflammatory reaction in the stomach and duodenum, yet the mechanism of this inflammatory infiltrate is unknown. the ability of helicobacter pylori to secrete a factor that attracts leucocytes is investigated. helicobacter pylori conditioned supernatant attracted neutrophils and monocytes with 50-100% of the activity of control chemotactic factor, 10(-8) m formyl-methionine-leucine-phenylalanine. strains derived from individuals with ulcer or non-ulcer a ...	1992	1398224
effect of helicobacter pylori infection on 24 hour intragastric acidity in patients with gastritis and duodenal ulcer.	helicobacter pylori status, gastric histology, and 24 hour acidity were studied in 35 gastritis patients, 21 duodenal ulcer patients, and 14 subjects with normal gastric mucosa. h pylori was identified in 21 of 35 patients with chronic active gastritis and in 19 of 21 duodenal ulcer patients, but in none of those with normal gastric mucosa. mean scores of activity of gastritis were similar in h pylori positive gastritis and duodenal ulcer patients, but were significantly lower in h pylori negati ...	1992	1398225
helicobacter pylori and gastric cancer: correlation with gastritis, intestinal metaplasia, and tumour histology.	this study aimed to examine the association between helicobacter pylori, histological gastritis, and intestinal metaplasia in gastric cancers of different histological types. a total of 169 gastrectomy specimens received in one pathology department were studied. altogether 156 were adenocarcinomas (intestinal type 87, diffuse type 50, mixed type 19). gastritis occurred in 137 of 163 body specimens (84%) and in 126 of 131 antral specimens (96%). its presence was unrelated to tumour histology. atr ...	1992	1398226
[chronic gastritis--does it need treatment and is it curable?].	fundamental considerations: addition to our medical knowledge of chronic gastritis and its sequelae; changes in nomenclature. methods/content: presentation of the pathophysiological situation (processes) oft colonization of the gastric mucosa by helicobacter pylori with particular consideration being given to histomorphological findings. discussion of the relative risk of gastritis patients contracting a gastric or duodenal ulcer, or possibly developing carcinoma of the stomach. conclusions: on ...	1992	1398409
lipid a in helicobacter pylori.	free lipid a of helicobacter pylori was characterized with regard to chemical composition, reactivity with anti-lipid a antibodies, and activity in a limulus lysate assay. the predominant fatty acids of h. pylori lipid a were 3-oh-18:0, 18:0, 3-oh-16:0, 16:0, and 14:0. hexosamine was present in amounts similar to those in campylobacter jejuni or salmonella typhimurium lipid a. the lipopolysaccharide of h. pylori contained 2-keto-3-deoxyoctonic acid, a common constituent of enterobacterial and c. ...	1992	1398948
[omeprazole].		1992	1399395
activity of the antimycotic ketoconazole against helicobacter pylori.		1992	1399937
construction of a helicobacter pylori genome map and demonstration of diversity at the genome level.	genomic dna from 30 strains of helicobacter pylori was subjected to pulsed-field gel electrophoresis (pfge) after digestion with noti and nrui. the genome sizes of the strains ranged from 1.6 to 1.73 mb, with an average size of 1.67 mb. by using noti and nrui, a circular map of h. pylori ua802 (1.7 mb) which contained three copies of 16s and 23s rrna genes was constructed. an unusual feature of the h. pylori genome was the separate location of at least two copies of 16s and 23s rrna genes. almos ...	1992	1400229
specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction.	development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ...	1992	1400961
serological detection of helicobacter pylori by a flow microsphere immunofluorescence assay.	a flow cytometric immunofluorescence assay (fmia) for the detection of immunoglobulin g antibodies to helicobacter pylori was developed. a multicomponent antigen was prepared and used to coat carboxylated polystyrene microspheres for reaction with patient sera followed by fluorescein isothiocyanate-labelled goat anti-human immunoglobulin g. the reacted microspheres were collected with a flow cytometer, and fluorescence was quantitated relative to the cutoff value provided by pooled sera from pat ...	1992	1400995
diagnostic tests for helicobacter pylori: comparison and influence of non-steroidal anti-inflammatory drugs.	to evaluate the efficacy of culture, histology, clo-test, helico-g and pyloriset tests in diagnosing helicobacter pylori in the presence or absence of non-steroidal anti-inflammatory drugs (nsaids).	1992	1401184
expression of 120 kilodalton protein and cytotoxicity in helicobacter pylori.	antral biopsy culture supernatants from 14 subjects with chronic gastritis, known to have iga antibodies to the 120 kilodalton protein, showed positive recognition of this antigen in western blots against a cytotoxin positive strain of helicobacter pylori but gave negative reactions with two cytotoxin negative strains. control immunoblots with culture supernatants from 13 non-responders to the protein were all negative. this indicates a direct association between expression of the 120 kilodalton ...	1992	1401190
selected cryopreservatives for long term storage of helicobacter pylori at low temperatures.	to meet the need for information on cryopreservation, a study was done on 32 helicobacter pylori strains, comparing different cryopreservative media. sheep blood, horse blood, horse serum with and without glycerol, and mineral oil media were used for long term storage of h pylori at -70 degrees c or in liquid nitrogen. procedures were developed which permitted recovery of 87.5% of the strains included in the study after they had been stored for 24 months. of those strains stored for more than th ...	1992	1401191
successful freeze storage and lyophilisation for preservation of helicobacter pylori.	long term storage techniques for the preservation of helicobacter pylori were developed. the cells survived at -75 degrees c in the presence of glycerol and at +4 degrees c after freeze-drying. both techniques are suitable for routine use.	1992	1401192
quantitative assessment of histological changes in chronic gastritis after eradication of helicobacter pylori.	to evaluate the effect of 10 day triple treatment on h pylori eradication and associated gastritis.	1992	1401210
potentiation of helicobacter pylori vacuolating toxin activity by nicotine and other weak bases.	about 50% of helicobacter pylori isolates produce a vacuolating toxin in vitro, which may be an important determinant of virulence. because ammonium salts potentiate h. pylori toxin activity, the effect of other weak bases upon toxin activity was determined. vacuolation of hela cells was quantitated using a neutral red uptake assay. as expected, ammonium chloride, trimethylamine, triethanolamine, and nicotine each induced vacuolation of hela cells when tested independently. in addition, each of ...	1992	1402018
[proton pump blockade and helicobacter pylori: status of current knowledge and clinical importance].		1992	1406011
alcohol dehydrogenase mediated acetaldehyde production by helicobacter pylori--a possible mechanism behind gastric injury.	two standard helicobacter pylori strains showed significant cytosolic alcohol dehydrogenase activity and produced considerable amounts of acetaldehyde when incubated with an ethanol containing solution in vitro. the alcohol dehydrogenase activity of the helicobacter pylori strains was almost as high as that found in klebsiella pneumoniae and far greater than that in escherichia coli or campylobacter jejuni. the amount of acetaldehyde produced by cytosol prepared from helicobacter pylori exceeded ...	1992	1406052
[current methods for the laboratory diagnosis of campylobacteriosis].	an analytical review of recent publications of home and foreign authors on the problem of laboratory diagnosis of campylobacteriosis is presented. the commercial nutrient media, methods of creation of the microaerophilic conditions for cultivation of campylobacter are presented. the filtration method is preferable for isolation of these agents from the studied material highly contaminated by accompanying microflora. a special attention is paid to immunodiagnosis of campylobacteriosis: agglutinat ...	1992	1406380
contribution of the 13c-urea breath test to the detection of helicobacter pylori gastritis in children.	serology, 13c-urea breath test, histology, campylobacter-like organism testing, and culture were performed in 95 consecutive children to evaluate the contribution of these tests to the detection of helicobacter pylori infection. in analyses considering any combination of three positive tests as "gold standard" for diagnosing h pylori infection, 26 children were helicobacter positive (27%), which is only one patient more than the number of children with only a positive culture. the accuracy of cu ...	1992	1408517
dna diversity among clinical isolates of helicobacter pylori detected by pcr-based rapd fingerprinting.	the rapd (or ap-pcr) dna fingerprinting method was used to distinguish among clinical isolates of helicobacter pylori, a bacterium whose long term carriage is associated with gastritis, peptic ulcers and gastric carcinomas. this method uses arbitrarily chosen oligonucleotides to prime dna synthesis from genomic sites to which they are fortuitously matched, or almost matched. most 10-nt primers with > or = 60% g + c yielded strain-specific arrays of up to 15 prominent fragments, as did most longe ...	1992	1408828
triple therapy with sucralfate is as effective as triple therapy containing bismuth in eradicating helicobacter pylori and reducing duodenal ulcer relapse rates.	duodenal ulcer relapse rates after therapy with sucralfate or bismuth are lower than those after h2-receptor antagonist therapy. this may be mediated by an antibacterial effect of these drugs on helicobacter pylori. bismuth has become an integral part of 'triple therapy' because of its documented anti-h. pylori effect. in vitro and clinical data suggest that sucralfate may also have an anti-h. pylori effect. the aim of this randomized, prospective therapeutic trial was to compare the efficacy of ...	1992	1411294
prevention of peptic ulcer relapse by sucralfate: mechanisms of action.	sucralfate has a complex effect on the luminal and mucosal environment of the stomach and duodenum. some of the actions are important in ulcer healing whilst others are important in preventing subsequent ulcer relapse. although sucralfate has little direct effect on acid secretion, there is evidence that after ulcer healing with this drug, parietal cell responsiveness is reduced. this may in part be mediated by increased somatostatin release from gastric d cells and may be important in reducing ...	1992	1411295
cell surface proteins of helicobacter pylori as antigens in an elisa and a comparison with three commercial elisa.	cell surface proteins of helicobacter pylori were solubilized by extraction with acidic glycine buffer, n-octyl-glucoside, lithium chloride, and distilled water, and by sonication. the preparations were evaluated as antigens in elisa to detect serum igg responses in patients and healthy subjects. sds-page analyses of the preparations from a type strain (nctc 11637) and of acidic glycine extracts of 4 clinical isolates showed multiple protein bands. the sera were classified as hp+ve and hp-ve by ...	1992	1411312
[comparison of the efficacy of omeprazole/bismuth subcitrate or triple therapy in helicobacter pylori gastritis. a prospective controlled study].	in a controlled, prospective, randomized trial, 10 patients with helicobacter pylori positive gastritis were treated either with triple therapy (tetracycline, ornidazole, bismuth subcitrate; t, or, cbs), or omeprazole/cbs (o/cbs) to test the eradication rate of each treatment, its effect on gastritis and meal stimulated gastrin release.	1992	1411403
clinical value of endoscopy and histology in the diagnosis of duodenogastric reflux disease.	the endoscopic observation of a bile lake in the stomach, antral gastritis, or ulcerations and the histologic finding of foveolar hyperplasia or chronic gastritis have been implicated as indicators of excessive duodenogastric reflux. the accuracy of these criteria was evaluated in 135 patients with nonspecific symptoms in the foregut suggestive of duodenogastric reflux and no evidence for alcohol- or drug-induced gastric mucosal injury.	1992	1411953
[helicobacter pylori and gastrointestinal tract diseases].	we review the bacteriological, epidemiological and pathogenetic aspects of helicobacter pylori and related diseases of the gastrointestinal tract. it is concluded that h. pylori seems to be at least one of several etiological factors for diseases such as chronic gastritis and duodenal ulcer. it may also be an important cofactor in the development of gastric carcinoma.	1992	1412235
[diagnosis of helicobacter pylori infection. rapid urease test, microscopy of smears and culture from ventricular biopsy compared with the 14c-urea breath test].	by employing the 14c-urea breath test as the reference method we determined the specificity and sensitivity of three bioptic methods for diagnosis of helicobacter pylori infection in 103 subjects. all biopsy specimens were obtained from the gastric antrum. for culture the specificity was 100%. its applicability was reduced, however, by a low sensitivity (73.8%) and a delay of several days before the final result was available. microscopy of loeffler-stained biopsy smears yielded a specificity of ...	1992	1412236
[detection of helicobacter pylori infections by antibody determination].		1992	1413904
helicobacter pylori and recurrence of duodenal ulcers.		1992	1415088
smoking does not contribute to duodenal ulcer relapse after helicobacter pylori eradication.	cigarette smoking is believed to be one of the major factors influencing duodenal ulcer (du) recurrence. however, the influence of cigarette smoking on du recurrence after the eradication of helicobacter pylori has not been separately addressed. the aim of this study was to investigate du relapse rate in smokers and nonsmokers, both with confirmed eradication of h. pylori. patients with h. pylori eradication, demonstrated at endoscopy 4 wk post-treatment, were included in the study. smoking hist ...	1992	1415092
nonsteroidal antiinflammatory drug-associated gastric ulcers do not require helicobacter pylori for their development.	nonsteroidal antiinflammatory drugs (nsaids) have been linked with a high incidence of ulcer complications. histologic gastritis is present in most patients with standard peptic ulcers, and this gastritis is generally related to helicobacter pylori (hp). we questioned whether gastric ulcers associated with nonsteroidal antiinflammatory drugs (nsaids) develop via a novel mechanism, distinct from the usual hp-gastritis-ulcer diathesis. two groups of patients with newly discovered gastric ulcers we ...	1992	1415094
helicobacter pylori-negative gastric ulcer.	causes of helicobacter pylori (hp)-negative gastric ulcers (gus) have not previously been systematically studied. in this study we examined possible causes of hp-negative gus. among 115 consecutive patients with endoscopic diagnoses of gu, 71 (62%) had hp infection, and 44 (38%) were found to be hp-negative. of the 71 hp-positive patients, 47 (66%) had no other detectable causal factors, 21 (30%) were regularly taking nonsteroidal antiinflammatory drugs (nsaids), and three (4%) had malignant gu. ...	1992	1415095
bleeding from a meckel's diverticulum after the use of ibuprofen.	a meckel's diverticulum is an unusual site of gastrointestinal bleeding in adults. bleeding often results from ulceration of ileal mucosa that lies adjacent to ectopic gastric mucosa. we report on a 27-yr-old man who bled from a meckel's diverticulum after receiving oral ibuprofen, a nonsteroidal antiinflammatory drug. examination of the resected diverticulum revealed fundal-type gastric mucosa with inflammation, submucosal hemorrhages, erosions and ulceration in the absence of helicobacter pylo ...	1992	1415106
source of helicobacter pylori infection: studies in abattoir workers and pigs.		1992	1415120
decreased level of antibodies against helicobacter pylori in patients with rheumatoid arthritis receiving intramuscular gold.	sodium aurothiomalate has been reported to have in vitro activity against helicobacter pylori. intramuscular gold, as given to patients with rheumatoid arthritis (ra), may therefore influence the colonisation of the gastric mucosa with h pylori.	1992	1417132
effect of helicobacter pylori lipopolysaccharide on the synthesis of sulfated gastric mucin.	the effect of h. pylori lipopolysaccharide on the synthesis and secretion of sulfated mucus glycoprotein in gastric mucosa was investigated. the lipopolysaccharide, while showing no discernible effect on the apomucin synthesis, exerted a profound inhibitory effect on the process of mucus glycoprotein glycosylation and sulfation, and evoked a rapid (within 15 min) inhibition (65%) in both mucin glycosylation and sulfation at its optimal concentration of 100 micrograms/ml. the data on mucin secret ...	1992	1417902
experience with 'triple' anti-helicobacter pylori eradication therapy: side effects and the importance of testing the pre-treatment bacterial isolate for metronidazole resistance.	at the 1990 world congresses of gastroenterology, the working party on helicobacter pylori (h. pylori) recommended that, in suitable patients, the bacterium should be eradicated using a therapeutic regimen comprising a bismuth salt, tetracycline and metronidazole for two weeks. we have treated 40 patients infected with h. pylori with 'triple' therapy consisting of 120 mg tripotassium dicitrato bismuthate q.d.s., 500 mg tetracycline q.d.s. and 400 mg metronidazole t.d.s. for two weeks. the succes ...	1992	1420735
short report: cefprozil for the eradication of helicobacter pylori infection.	helicobacter pylori infection has proven to be extraordinarily difficult to eradicate. antimicrobial monotherapies have been particularly disappointing, with most eradication rates in the range of 0 to 15%. we evaluated cefprozil (250 mg q.d.s. for 14 days) in 12 h. pylori-infected subjects. the 13c-urea breath test was used to evaluate effectiveness of therapy. eradication was defined as a negative urea breath test 4 to 6 weeks after the end of treatment. suppression of h. pylori was demonstrat ...	1992	1420742
clinicopathological assessment of gastric biopsy samples of patients with helicobacter pylori infection--metronidazole resistance and compliance problems in the united arab emirates.	the significance of helicobacter pylori (hp) infection was assessed prospectively in forty-two patients with dyspepsia using histological, bacteriological and biopsy urease techniques. thirty-eight patients (90.5%) were positive for hp infection and were treated with bismuth subcitrate (de nol), tinidazole and doxycycline. hp was present in the antrum, corpus, fundus, duodenum and gastric juice in 36, 26, 23, 2 and 2 patients respectively (p < 0.01, x2 test). histological assessment yielded more ...	1992	1421445
prevalence of gastritis in patients with acromegaly: untreated and during treatment with octreotide.	it has previously been suggested that acromegalic patients treated with the somatostatin analogue octreotide invariably have chronic gastritis. we have examined the prevalence of gastritis in a large group of acromegalic patients, untreated and during treatment with octreotide.	1992	1424205
[helicobacter pylori-associated gastritis and low-malignancy malt lymphoma].		1992	1425268
[helicobacter pylori: the local iga response of the gastric mucosa].		1992	1425270
[helicobacter pylori and gastric carcinoma].		1992	1425302
evaluation of a monoclonal antibody for detection of helicobacter pylori in a direct immunofluorescence test.	a monoclonal antibody was developed for detection of helicobacter pylori in gastric tissue sections in a direct immunofluorescence test. on a comparison of the immunofluorescence test with standard methods for detection of helicobacter pylori, i.e. culture, the urease activity test and histological examination of tissue sections, using 158 biopsy specimens, 30 specimens were positive in all methods and 64 negative. in the remaining cases comparison was not possible because either immunofluoresce ...	1992	1425734
surface hydrophobicity of gastric mucosa in helicobacter pylori infection: effect of clearance and eradication.	surface hydrophobicity of the gastric mucosa is reduced in peptic ulcer disease and helicobacter pylori infection. this abnormality may be caused by h. pylori or may be an inherent defect. the aim of the present study was to clarify the relationship between h. pylori infection and mucosal hydrophobicity by examining the effect of eradication of the organism. h. pylori-positive patients with (n = 42) or without (n = 42) duodenal ulcer were randomized to receive ranitidine, bismuth, or bismuth plu ...	1992	1426866
chronic active gastritis due to helicobacter pylori in immunized gnotobiotic piglets.	in gnotobiotic piglets, parenteral vaccination with formalin-killed helicobacter pylori and oral vaccination with live bacteria induced h. pylori-specific serum immunoglobulins g, m, and a. vaccination reduced but did not prevent infection by subsequent challenge with viable h. pylori. oral vaccination with killed bacteria was less effective in inducing serum antibody and had no effect on bacterial colonization. immunization status influenced the histologic response of piglets to challenge by h. ...	1992	1426878
eradication of helicobacter pylori reduces gastric and duodenal ulcer recurrence.		1992	1426890
mucosal peptic activity during helicobacter pylori infection in pediatric patients.	intramucosal peptic activity may participate in the genesis of acute and chronic superficial gastritis. the proteolytic activity of homogenates of gastric mucosa (antrum and body) and duodenum were measured at ph 2.0 (total peptic activity) after exposure to ph 8.0 (pepsinogen) and the activated pepsinogen (pepsin) was calculated in pediatric patients investigated for the presence of helicobacter pylori (h pylori), 122 antral, 77 stomach body, and 74 duodenal biopsies were examined in 43 h pylor ...	1992	1427366
helicobacter pylori and peptic ulcer recurrence.		1992	1427384
helicobacter pylori and peptic ulcer recurrence.		1992	1427385
epithelial degeneration induced by helicobacter pylori.		1992	1427763
does helicobacter pylori cause gastric cancer?		1992	1428029
a study of helicobacter pylori in north indian subjects with non-ulcer dyspepsia.	a prospective case control study was conducted in 50 patients with non-ulcer dyspepsia (nud) and 10 age and sex matched controls to determine the prevalence of helicobacter pylori in patients with nud and to correlate symptoms, histology and presence of h pylori in gastric biopsies. endoscopic biopsies from antrum and fundus were subjected to urease test and histological examination. on histology, h pylori was identified in 27 (54%) nud patients and in one of 10 controls. urease test was positiv ...	1992	1428036
[dynamics of neutrophils in the gastric mucous layer of helicobacter pylori associated chronic gastritis].		1992	1431391
surfactant-like material in the antral gastric mucosa of children.	it has been proposed that the hydrophobicity of the gastric mucosa is due to surfactant-like material (slm), mainly composed of phospholipid, adsorbed to the mucosal surface. biochemical and physiological studies have been performed in different mammals but morphological data on children are lacking. in the present work, the presence of slm on the antral gastric mucosa of children has been studied by electron microscopy. the results demonstrate that slm is present and shows two different forms, ...	1992	1432465
[prognostic significance of cytochemical and functional activity of blood lymphocytes in patients with peptic ulcer and the presence of helicobacter pylori].		1992	1434395
colloidal bismuth subcitrate and omeprazole inhibit alcohol dehydrogenase mediated acetaldehyde production by helicobacter pylori.	we have recently shown that helicobacter pylori possesses marked alcohol dehydrogenase (adh) activity and is capable--when incubated with an ethanol containing solution in vitro--of producing large amounts of acetaldehyde. in the present study we report that some drugs commonly used for the eradication of h. pylori and for the treatment of gastroduodenal diseases are potent adh inhibitors and, consequently, effectively prevent bacterial oxidation of ethanol to acetaldehyde. colloidal bismuth sub ...	1992	1435071
cloning and genetic characterization of a helicobacter pylori flagellin gene.	helicobacter pylori produces polar sheathed flagella, which are believed to be essential for the bacterial colonization of the human gastric mucosa. here we report on the cloning and genetic characterization of a h. pylori gene encoding the subunit of the flagellar filament, the flagellin. screening of a genomic library of h. pylori with an oligonucleotide probe derived from the n-terminal amino acid sequence of purified flagellin resulted in a recombinant plasmid clone carrying the flagellin-en ...	1992	1435261
[effect of colloid bismuth treatment on campylobacter pylori infection of the gastric mucosa and the clinical course of non-ulcer dyspepsia].	there are suggestions, that the idiopathic non-ulcer dyspepsia is related to the chronic gastritis type b coexisting with c. pylori infection. presented studies were aimed at the assessing of an effect of the treatment with de-nol on the course of the idiopathic non-ulcer dyspepsia of c. pylori infection. gastroscopic examination was performed in 52 patients with non-ulcer dyspepsia before and after the treatment with de-nol administered for 4-8 weeks. campylobacter pylori was isolated from 71% ...	1992	1437790
[inflammatory changes in the gastric mucosa of patients with idiopathic non-ulcer dyspepsia and the effect of colloid bismuth treatment on the course of inflammation].	the studies were aimed at the assessment of the coexistence of non-ulcer dyspepsia with chronic gastritis and campylobacter pylori infection, and of the effect of therapy with de-nol on the course of such disease. the studies involved 50 patients with non-ulcer dyspepsia. prior to and after the treatment with de-nol samples of the mucosa collected from the antrum and corpus of the stomach have been examined histologically with urease test indicating c. pylori infection. chronic gastritis of the ...	1992	1437791
eradication of helicobacter pylori: therapies and clinical implications.	this review presents a critical evaluation of the role of helicobacter pylori eradication in the management of peptic ulcer disease and non-ulcer dyspepsia. on current evidence, h. pylori eradication therapy seems likely to emerge as the most rational and cost-effective treatment for duodenal ulcer. the role of h. pylori eradication in the treatment of gastric ulcer and non-ulcer dyspepsia is unclear and requires further study. the emerging problem of antibiotic resistance in h. pylori is of maj ...	1992	1437952
[prevalence of helicobacter pylori in patients with gastric cancer in panama].	cancer of the stomach is the second in incidence in panama after the cancer of the prostata. it is studied now the incidence of infection due to helicobacter pylori in patients with gastric carcinoma. it seems to be a significative relationship between the prevalence of gastric carcinoma and h. pylori infections. this observation is important because by the similar therapeutic implications in virus dna hepatitis type b and hepatoma.	1992	1439007
[classification of gastritis according to the sydney system].		1992	1439431
effect of helicobacter pylori on parietal cell sensitivity to pentagastrin in duodenal ulcer subjects.	we have investigated the possibility that hypergastrinaemia in chronic helicobacter pylori infection is a compensatory response to reduced parietal cell sensitivity to gastrin. the acid response to 45-min infusions of pentagastrin at sequential doses (micrograms/kg/h) of 0, 0.031, 0.062, 0.124, and 0.6 was compared before and 1 month after eradication of h. pylori in eight duodenal ulcer patients. the median acid outputs (mmol/h) with the respective infusions were 5.0, 7.5, 26.5, 30.8, and 37.0 ...	1992	1439539
[the rational use of de-nol in the treatment of peptic ulcer and chronic active gastroduodenitis associated with helicobacter pylori].		1992	1440381
helicobacter pylori and non-ulcer dyspepsia.		1992	1440895
[the efficacy of treating peptic ulcer patients with trichopol and dalargin].	a study of 90 patients with endoscopically proved duodenal ulcer revealed that dalargin and trichopol+dalargin treatment resulted in early scarring of the ulcer and control of gastroesophageal and duodenogastric reflux in the majority of patients. gastroduodenitis symptoms were not eliminated in a half of all patients. it was shown that persistent manifestations of gastroduodenitis remained in patients with a pronounced pyloric helicobacteriosis and a high titer of antibodies to cells of the gas ...	1992	1441328
[therapy of stomach ulcer--a comparison between the low dosage antacid hydrotalcite and ranitidine--results of a randomized multicenter double-blind study. talcivent study group].	in a 8 week double-blind randomized multicenter trial in 159 patients with benign gastric ulcer the efficacy of hydrotalcite vs. ranitidine in expediting ulcer healing and in achieving pain relief was determined. 79 patients received hydrotalcite 1000 mg q.i.d. as tablets equalling a total neutralizing capacity of 111.2 mval and 80 patients received ranitidine (300 mg at night). endoscopically controlled healing rates after 4 weeks of therapy amounted to 41.8% with hydrotalcite and 53.8% with ra ...	1992	1441674
active autoimmune gastritis without total atrophy of the glands.	to date, autoimmune gastritis has been diagnosed for the most part only when total atrophy of the oxyntic glands is detected. on the basis of 40 patients without total atrophy of the glands, and with parietal cell antibodies in the serum, we show that the diagnosis of type a gastritis is also possible in the pre-atrophic stage. the histological criteria for the diagnosis of active autoimmune gastritis without total atrophy of the glands are 1. usually dense, diffuse locally emphasized lymphocyti ...	1992	1441676
helicobacter pylori, gastritis, and ulcers in pediatrics.	hp now appears to be more than a simple commensal organism in patients with gastritis or peptic ulcer disease. microbiologic, serologic, and epidemiologic studies all confirm that hp has an important role in children with abdominal pain. hp is found in the gastric mucosa of children with histologically proven gastritis or peptic ulcer. the organism can be transmitted from human to human with evidence of colonization, appearance of gastritis, and serum antibody response. antimicrobial therapy dir ...	1992	1442315
role of helicobacter pylori in gastritis and duodenitis in man.	although helicobacter pylori is now accepted as the major aetiological factor in chronic gastritis in man, many of the factors which determine its pathogenicity are unknown. the organism has adapted to survive in the low-ph environment of the stomach, partly through its ability to buffer hydrogen ion by the hydrolysis of urea and by the presence of lectins on its surface, which bind to gastric mucosa and epithelial cells. after attachment, harmful toxins and enzymes have access to the gastric ce ...	1992	1442334
h. pylori causes cancer: true or false?		1992	1442669
high prevalence and persistence of cytotoxin-positive helicobacter pylori strains in a population with high prevalence of atrophic gastritis.	helicobacter pylori is strongly associated with both chronic gastritis and duodenal ulcer. certain strains of h. pylori produce a vacuolating cytotoxin in vitro. at new orleans charity hospital, concentrated culture supernatants from 119 of 144 (83%) h. pylori strains isolated from 86 patients at high risk of developing gastric cancer, caused vacuolization in hela s3 cells. cytotoxin activity was neutralized by acid (ph 4) and basic (ph 10) solutions and proteases, and was precipitable by (nh4)2 ...	1992	1442673
prevalence and significance of helicobacter pylori in patients with barrett's esophagus.	a retrospective study for assessment of the prevalence of helicobacter pylori was performed in 107 consecutive patients with columnar-lined epithelium in the esophagus. the presence of hiatal hernia and esophagitis was noted. from 71 patients, biopsy specimens were available for analysis. no difference in the presence or absence of h. pylori was noted, regardless of whether or not there was esophagitis or hiatal hernia. most h. pylori-positive patients show a low bacterial load in the metaplasti ...	1992	1442681