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erythromycin for campylobacter jejuni-associated dysentery. 19873598790
treatment of campylobacter gastroenteritis.twin boys suffered from recurrent diarrhoea due to campylobacter jejuni after entering a day nursery. stool sampling of all 17 children at the nursery revealed c. jejuni in 12. simultaneous treatment with antibiotics of all children with positive cultures successfully eradicated the infection.19873813645
early treatment with erythromycin for campylobacter jejuni-associated diarrhea. 19873794884
campylobacter jejuni infections. 19873796409
campylobacter jejuni/campylobacter coli-associated guillain-barré syndrome. immunoblot confirmation of the serological response.immunoblot (western blot) examination of the sera of 45 patients with the guillain-barré syndrome demonstrated that between three and five immunoreactive bands that were characteristic of a recent campylobacter jejuni/campylobacter coli infection were present in 22 of these patients. the immunoblots paralleled the serological response that is found in campylobacter enteritis and confirmed the specificity of our previous enzyme-linked immunosorbent assays (elisas), which indicates that antecedent ...19873626926
enteric campylobacter infection in gnotobiotic calves and lambs.gnotobiotic calves and lambs were infected orally with campylobacter jejuni, c coli or c hyointestinalis to assess pathogenicity. all animals were successfully colonised and excreted mucoid faeces but showed no other clinical signs. campylobacters colonised the large intestine better than the small intestine, in which bacterial numbers decreased with time after infection. campylobacters were found occasionally in the lumen of crypts in close proximity to epithelial cells and included in a mucus- ...19873628988
[clinical characteristics of acute intestinal diseases caused by campylobacter jejuni]. 19873629390
in vitro susceptibility of campylobacter jejuni and campylobacter coli isolated in denmark to fourteen antimicrobial agents.the in vitro susceptibility of 95 recent danish human clinical isolates of campylobacter jejuni and campylobacter coli to 14 antimicrobial agents was determined by an agar dilution technique. c. jejuni and c. coli were considered together. doxycycline, ciprofloxacin, gentamicin, clindamycin, nitrofurantoin and erythromycin were the most active drugs. 2% of the strains tested were resistant to erythromycin (mic greater than 3.1 micrograms/ml). therefore susceptibility testing is suggested if anti ...19873630712
predictive value of stool examination in acute diarrhea.we prospectively evaluate the value of fecal blood and fecal leukocytes in predicting whether acute diarrhea in adults is associated with a stool culture positive for a bacterial pathogen. one hundred thirteen patients, aged 19 to 50 years, seen in a two-year period in an urban adult outpatient setting underwent stool culture for the presenting symptom of diarrhea. heterosexual men represented 48% of the cohort, women represented 17%, and homosexual men represented 35%. overall, 53 (47%) of the ...19873632285
evaluation of gamma radiation levels for reducing pathogenic bacteria and fungi in animal sewage and laboratory effluents.sewage samples collected from animal wastes and from effluents at an animal disease laboratory were inoculated with known numbers of pathogenic organisms and subjected to various doses of gamma radiation from a 60co source. surviving test organisms were quantitatively determined by selective and enrichment techniques. the experiment was modeled as a quantal assay in which probit analysis was applied to obtain d10 values. the d10 value represents the irradiating dose required to reduce the popula ...19873651881
production of a unique cytotoxin by campylobacter jejuni.campylobacter jejuni is an important diarrheal pathogen worldwide; the mechanisms by which it causes disease remain unclear. because of its association with inflammatory diarrhea, we postulated that c. jejuni might produce a cytotoxin similar to that produced by shigella sp., enterohemorrhagic escherichia coli o157, or clostridium difficile. filtrates of 12 polymyxin-treated isolates of c. jejuni were placed on hela cells (sensitive to shiga toxin cytotoxicity) and chinese hamster ovary (cho) ce ...19873653987
naturally occurring auxotrophs of campylobacter jejuni and campylobacter coli.the nutritional requirements for 439 campylobacter jejuni isolates and 46 campylobacter coli isolates were determined by using a previously described chemically defined medium, campylobacter defined medium. with this medium, 45% of both human and nonhuman c. jejuni isolates demonstrated auxotrophic requirements. none of the 46 c. coli isolates studied demonstrated requirements for amino acids on campylobacter defined medium. the most common auxotrophic requirement among c. jejuni isolates was fo ...19873654939
rapid identification of campylobacter pylori (c. pyloridis) by preformed enzymes.campylobacter pylori (c. pyloridis) is a fastidious organism found in the gastric mucosa associated with histological gastritis and peptic ulceration. a rapid identification scheme that detects the presence of preformed enzymes (rosco diagnostica, taastrup, denmark) was applied to clinical isolates of c. pylori. the isolates tested were a very homogeneous group. they all produced oxidase, catalase, urease, alkaline phosphatase, gamma-glutamyl aminopeptidase, leucine aminopeptidase, and dnase. no ...19873654941
prevalence and characterization of hippurate-negative campylobacter jejuni in king county, washington.a total of 593 strains of thermophilic campylobacter species were isolated either from humans with diarrhea or from poultry in king county, washington. of these strains, 98 (52 hippurate-positive strains and all 46 of the hippurate-negative strains) were selected for further phenotypic characterization and genetic classification. hippurate hydrolysis, the test typically used to differentiate campylobacter jejuni and c. coli, did not always correlate with the genetic classification. all hippurate ...19873654945
rapid and improved gas-liquid chromatography technique for detection of hippurate hydrolysis by campylobacter jejuni and campylobacter coli.a gas-liquid chromatographic method which requires no chloroform extraction of the split products has been investigated for the detection of hippurate hydrolysis by campylobacter spp. this technique gave better reproducibility than other tests also used in this study and allows the routine use of the gas-liquid chromatographic method for identification of campylobacter isolates.19873654950
problems in identification of campylobacter jejuni associated with acquisition of resistance to nalidixic acid.in a patient with campylobacteriosis, we observed development of resistance to nalidixic acid and norfloxacin under adequate treatment with the latter antibiotic. isolates before and after treatment differed in their total protein profile but were otherwise identical with respect to enzymatic activity, serotype, crude membrane protein profiles, and other phenotypic characteristics.19873654954
serological study of campylobacter jejuni infection in slaughterhouse workers. 19873655409
counterimmunoelectrophoresis compared with complement fixation and passive haemagglutination tests in the evaluation of the immune response in campylobacter infections.the immune response was studied in 238 human patients with campylobacter jejuni/coli (cjc)-infections in rotterdam by the counterimmunoelectrophoresis (cie) test, a commercial complement fixation test (cft) and the passive haemagglutination test (ha). antibodies became detectable in the three tests around 4 days after the onset of complaints. between the 7th and the 20th days after onset of illness 79%, 80% and 53% of the patients demonstrated antibodies by the cie, the cft and the ha, respectiv ...19873662488
effect of sub-inhibitory concentrations of antibiotics on adhesion of campylobacter jejuni in vitro.the effects of sub-inhibitory concentrations (mic 50 and mic 25) of clindamycin, erythromycin and chloramphenicol on the adherence of campylobacter jejuni to int 407 cells were studied. the results indicated that the adhesion was inhibited at various extents, mostly by mic 50, and that the inhibition increased with time. the interpretation of our data is that the antibiotics can interfere with the adhesin(s) synthesis.19873663336
[a study on the epidemiological features and serotype distribution of campylobacter jejuni enteritis in children]. 19873664621
campylobacter jejuni: a common cause of gastroenteritis in rural ireland. 19873667193
pathology of campylobacter jejuni abortion in sheep.campylobacter jejuni was inoculated intravenously into pregnant ewes on gestation days 114 and 123 to reproduce ovine abortion. all ewes aborted 7-12 days post-inoculation. high numbers of c. jejuni were isolated from ewe tissues (caruncle, bile, cecal feces), fetal tissues, and placenta. c. jejuni colonies were identified in caruncles and placenta by light microscopy and immunoperoxidase techniques. histologically, inoculated ewes had a severe purulent endometritis with vasculitis. placentas fr ...19873672807
factors affecting the survival of campylobacter jejuni in relation to immersion scalding of poultry.potential measures for reducing the survival of campylobacters during commercial scalding of poultry have been evaluated in a series of laboratory trials. at 50 degrees c, the lower temperature limit of commercial scalding, raising the ph of a buffered heating medium from 6.0 to 9.0 markedly increased the heat sensitivity of campylobacter jejuni but the effect was largely nullified in the presence of 1 per cent 'organic material' (50:50 horse blood and milk). either in the presence or absence of ...19873672830
guillain-barré syndrome following campylobacter jejuni enteritis. 19873675255
survival of thermophilic campylobacters on fingertips and their elimination by washing and disinfection.a simple impression-plate technique has been used to investigate the survival of four thermophilic campylobacter strains applied to fingertips. campylobacters suspended in 0.1% peptone water and dried on the fingertips survived for different periods of time ranging from less than 1 to greater than or equal to 4 min. however, campylobacters suspended in chicken liquor or blood survived for much longer periods. the most resilient organism was campylobacter jejuni nctc 11392 which, when suspended i ...19873678391
serogroups of thermophilic campylobacters from humans and from non-human sources, israel 1982-1985.the distribution of serogroups of thermophilic campylobacters isolated in israel from human patients (2421 isolates), chicken (942), turkeys (158), cattle (398), wild birds (234) and other sources, was studied. among the human isolates, 74 rog-serogroups were identified. the six most commonly isolated of these (1, 18; 11; 12; 8,23; 4 and 5,39) were found frequently in chickens. only four common serogroups in man were also common in cattle, three in turkeys and two in wild birds. two common serog ...19873678392
[optimal conditions and affecting factors on adhesion of campylobacter jejuni to hela cells]. 19873679170
serotypes of campylobacter jejuni isolated from patients attending a diarrhoeal disease hospital in urban bangladesh.the serotypes of strains of campylobacter jejuni isolated from patients attending the international center for diarrhoeal disease research, bangladesh (icddr, b), dhaka, and from animals were ascertained by penner and hennessy's (1980) serotyping scheme. of 102 isolates from man, 74% were typable and serotypes 53, 15 and 22 predominated. of 26 isolates from animals, 65% were typable and serotypes 15 and 53 occurred frequently. the diarrhoeal illnesses associated with different serotypes were sim ...19873694663
cytotoxic and enterotoxic activities of campylobacter jejuni are not specified by tetracycline resistance plasmids pmak175 and pua466.the 45-kilobase tetracycline resistance plasmids pmak175 and pua466 from campylobacter jejuni were examined using curing and mating experiments. however, these plasmids encoded neither cytotoxin production, as determined in vero cells, nor enterotoxin activity, as determined in chinese hamster ovary cells.19873793867
evaluation of four enrichment media for isolation of campylobacter jejuni.diarrheal stool specimens were inoculated into the following media: alkaline peptone water (apw), bruce-zochowsky medium (bz), campylobacter enrichment broth (ceb), campy-thio broth (ct), and skirrow blood-agar (sk) plate. all media were incubated at 42 degrees c in microaerophilic conditions for 24 h. afterwards, a new sk plate was inoculated from every liquid medium. campylobacter jejuni was isolated from 43 of the 259 specimens when ct was used, from 45 when apw was used, from 46 when bz was ...19873793873
characterization and expression of a cloned tetracycline resistance determinant from campylobacter jejuni plasmid pua466.a tetracycline resistance (tcr) determinant previously cloned from the campylobacter jejuni plasmid pua466 (d. e. taylor, j. bacteriol. 165:1037-1039, 1986) was localized by restriction endonuclease mapping, subcloning, and tn1000 insertion mutagenesis to a 2-kilobase region consisting of 1.8- and 0.2-kilobase hincii fragments. tcr encoded by the cloned fragment (puoa1) was expressed constitutively in escherichia coli. a protein with an apparent molecular weight of 68,000 encoded by puoa1 was pr ...19873036765
antimicrobial resistance of campylobacter jejuni and campylobacter coli with special reference to plasmid profiles of japanese clinical isolates.a total of 111 clinical isolates of campylobacter jejuni and 10 clinical isolates of campylobacter coli were characterized by their susceptibility to nine antimicrobial agents and by their plasmid profiles on agarose gel electrophoresis. all of the c. jejuni isolates were susceptible to chloramphenicol, ciprofloxacin, erythromycin, kanamycin, and nalidixic acid, but 55% were tetracycline resistant. in the 10 c. coli isolates, a high prevalence of multiple-antibiotic resistance was noted. plasmid ...19873038005
two-year survey of etiologic agents of diarrheal disease at san lazaro hospital, manila, republic of the philippines.the prevalence of bacterial pathogens and rotavirus in 2,908 patients with diarrhea who were admitted to san lazaro hospital in manila in 1983 and 1984 was determined. one or more enteric pathogens were isolated or detected in samples from 1,698 (58.4%) patients. isolation rates for the various enteropathogens were as follows: rotavirus, 30.6%; shigella spp., 11.6%; salmonella spp., 9.2%; enterotoxigenic escherichia coli (1983 only), 7.8%; vibrio cholerae biotype eltor, 3.8%; non-o1 v. cholerae, ...19873038946
[rotavirus infection in the older child and adult].rotaviruses were detected in 1.3% of more than 10,000 stool samples from children--older than 6 years--and adults. beside rotaviruses the main enteropathogenic agents found were salmonellae (6.3%) and campylobacter jejuni/coli (1.7%). similar as the rotavirus infection of infants, in adults symptoms and signs were nearly the same except that the disease was not so severe. a correlation of the rotavirus infection with certain behaviour or eating habits was not found.19872830184
gene transfer from escherichia coli to campylobacter species: development of shuttle vectors for genetic analysis of campylobacter jejuni.a shuttle cloning vector (pil550) has been constructed which can be mobilized from escherichia coli to campylobacter jejuni, campylobacter coli, and campylobacter fetus by complementation with the transfer functions of an incp plasmid in trans, with a frequency of 10(-4) transconjugants per donor. we also present evidence for a dna modification system in c. jejuni.19872822671
cloning and expression of a tetracycline resistance determinant from campylobacter jejuni in escherichia coli.the tetracycline resistance gene (tet) from the campylobacter jejuni plasmid pfkt1025 was cloned into both puc18 and pbr322 and was expressed when the chimeric plasmids were introduced into escherichia coli. the location of the tet determinant on the chimeric plasmids was determined by bal 31 deletion mapping within a 2.25-kilobase (kb) rsai-hincii fragment. a protein of approximately 70 kilodaltons was consistently produced by e. coli maxicells harboring the cloned tet determinant. a 500-base-p ...19872823694
rotaviruses in infants with diarrhea studied by viral rna electrophoresis in ankara, turkey.incidence of rotavirus in children under 2 years of age, admitted to 2 children's hospitals i n ankara, turkey, from july 1984-june 1985 was measured using viral rna electrophoresis in agarose gels. 375 children were selected at random from over 14,000 diarrhea admissions, and their stool specimens taken on admission were compared to those of 333 children without diarrhea. 61 (16.3%) of the diarrhea cases had detectable rotavirus, compared to 0% in controls. bacteriologic tests for salmonella ...19872851886
an outbreak of campylobacter jejuni infection in a neonatal intensive care unit.during a period of 2 weeks campylobacter jejuni infection was diagnosed in seven infants in a neonatal intensive care unit. an identical serotype of camp. jejuni was found in five of the cases. investigations suggested that a common source of infection or infection acquired from mothers during delivery were unlikely. the dates of onset and the common serotype indicate that the outbreak may have been caused by person-to-person spread.19872880901
urine and faecal iga response during naturally acquired infection with campylobacter jejuni. 19872883461
raw beef, pork and chicken in japan contaminated with salmonella sp., campylobacter sp., yersinia enterocolitica, and clostridium perfringens--a comparative study.one hundred and twenty samples each of raw ground beef, pork and chicken from ten local grocery stores in shimane prefecture, japan, were examined for the presence of salmonella sp. (sal), campylobacter jejuni (cj), campylobacter coli (cc), yersinia enterocolitica (ye), and clostridium perfringens (cp) from april 1984 to march 1985. a total of 205 isolates of sal (112 strains), cj (64 strains), cc (one strain), ye (7 strains) and cp (21 strains) were recovered from 17 beef (14.2%), 31 pork (25.8 ...19872887078
potential pathogenic mechanisms of animal-passed virulence-enhanced strains of campylobacter jejuni/coli. 19873108554
[comparative in vitro activity of 10 new 4-quinolones against enteropathogenic bacteria].the susceptibility has been determined of 169 shigella spp., 93 salmonella spp., 68 yersinia enterocolitica, 34 vibrio cholerae 0:1, and 34 campylobacter jejuni, isolated in central africa and in belgium, to ten fluoroquinolones: ciprofloxacin, ofloxacin, norfloxacin, pefloxacin, enoxacin, amifloxacin, ci-934, a-56619, a-56620 and ro 23-6240. all quinolones were active against the strains tested, showing an mic90 between 0.004 mg/l and 2 mg/l for strains susceptible to nalidixic acid and an mic9 ...19873116489
[campylobacter jejuni and its incidence in meat packaged under warm conditions].the problem of survival of campylobacter jejuni was studied in artificially contaminated butchered meat packed in warm condition. apart from the presence of campylobacter jejuni, the total number of microorganisms and the shelf life of the meat were studied. with plain packing, campylobacter jejuni survived six to seven days and the shelf life of the meat was about a week. in vacuum-packed meat campylobacter jejuni was isolated for ten to eleven days and sensory changes occurred after ten to fou ...19873118552
[campylobacter jejuni in poultry processed in slaughterhouses].the frequency of occurrence of campylobacter jejuni germs in dressed poultry was studied for a year. the samples--smears from the body cavities of chickens--were collected during the technological dressing of the chickens; 101 strains of campylobacter jejuni (i. e. 28.69%) were isolated from the 352 samples analyzed. the occurrence of the germs exhibited a considerable seasonal variance with peak rates in spring and summer. the use of a suitable culture medium, the technique of cultivation and t ...19873118554
antimicrobial activity of lysozyme against bacteria involved in food spoilage and food-borne disease.egg white lysozyme was demonstrated to have antibacterial activity against organisms of concern in food safety, including listeria monocytogenes and certain strains of clostridium botulinum. we also found that the food spoilage thermophile clostridium thermosaccharolyticum was highly susceptible to lysozyme and confirmed that the spoilage organisms bacillus stearothermophilus and clostridium tyrobutyricum were also extremely sensitive. several gram-positive and gram-negative pathogens isolated f ...19873118808
comparative in vitro activity of a-56268 (te-031) against gram-positive and gram-negative bacteria and chlamydia trachomatis.the in vitro activity of a-56268 (te-031) was determined and compared with that of 13 antibiotics against 401 gram-positive and gram-negative bacteria and 11 strains of chlamydia trachomatis. a-56268 was very active against methicillin-susceptible staphylococcus aureus and neisseria gonorrhoeae, and was among the most active of the agents tested against listeria monocytogenes, streptococci and chlamydia trachomatis. it was moderately active against haemophilus spp., vibrio spp., campylobacter je ...19872954818
the influence of immunity on raw milk--associated campylobacter infection.after a retreat to an oregon farm, 19 of 31 college students developed an acute gastrointestinal illness. campylobacter jejuni infection was recognized in all the ill students and caused asymptomatic infections in three others. in total, 22 (88%) of 25 students who consumed raw milk for the first time became infected as compared with none of two who had not consumed raw milk. among ten persons who chronically consumed raw milk, none was ill, a striking difference from the 76% attack rate among t ...19872431167
immunochemical approaches to gene probe assays.antibodies specific for helical nucleic acids can be applied to assays for hybridized dna and/or rna. the assays can use either radioactive or nonradioactive detection systems. antibodies specific for rna-dna hybrids are applicable to assays for measuring hybrid helices that are immobilized on plastic or nitrocellulose, whether the helices are preformed in solution or are formed on the solid-phase support. alternatively, anti-rna-dna hybrid antibodies can be immobilized and used to capture hybri ...19872437829
antigenic variation of campylobacter flagella.sodium dodecyl sulfate-polyacrylamide gel electrophoresis of flagella dissociated from strains of campylobacter coli and campylobacter jejuni belonging to the heat-labile serogroup lio 8 showed that some strains were capable of producing flagellin subunits of two different molecular weights (mrs), 59,500 and 61,500. immunoelectron microscopy of cultures of the type strain of this serogroup, c. coli vc167, showed the presence of two flagellum filaments of different antigenic specificity. epitopes ...19872444577
isolation and characterization of campylobacter flagellins.sequential acid ph dissociation, differential ultracentrifugation, and neutral ph reassociation were used to partially purify serotypically distinct flagella from three strains of campylobacter jejuni and the two antigenic phases of flagella of campylobacter coli vc167. each c. jejuni flagellin and c. coli vc167 antigenic phase 1 flagellin were purified to homogeneity by reverse-phase high-performance liquid chromatography with a c8 spheri-10 column. c. coli vc167 antigenic phase 2 was purified ...19872444578
[effect of nacl on the survival of campylobacter jejuni at a temperature of 20 degrees centigrade]. 19872981001
campylobacter jejuni: isolated from an aborted caprine fetus in ontario. 198717422911
phylogenetic diversity and position of the genus campylobacter.rna sequence analysis has been used to examine the phylogenetic position and structure of the genus campylobacter. a complete 5s rrna sequence was determined for two strains of campylobacter jejuni and extensive partial sequences of the 16s rrna were obtained for several strains of c. jejuni and wolinella succinogenes. in addition limited partial sequence data were obtained from the 16s rrnas of isolates of c. coli, c. laridis, c. fetus, c. fecalis, and c. pyloridis. it was found that w. succ ...198711542086
bacterial, parasitic and viral enteropathogens associated with diarrhoea in saudi children.in a 2-year study of stools from saudi children with gastro-enteritis and from controls, rotavirus was the pathogen most frequently detected, either alone (44.3%) or in combination with other enteropathogens (7%). there were two peaks for rotavirus isolates, one during the cold months and the other during the dry, hot season. salmonella spp. and campylobacter jejuni were the second and third most frequently isolated enteropathogens. enteropathogenic escherichia coli did not contribute significan ...19882456718
identification of an antigen common to different species of the genus campylobacter.affinity-purified antibody specific for a determinant on flagellin from campylobacter jejuni was used to screen by immunoblotting strains of c. coli, c. laridis, c. fetus, "c. upsaliensis," c. pylori, and c. sputorum biovar fecalis. the antigen was detected in each of these species, but the molecular weights of the proteins bearing the common antigen varied considerably.19882457601
campylobacters and impaired fetal development in mice.intravenous injection of eight human strains of campylobacter fetus ss fetus and campylobacter jejuni into mice at various stages of pregnancy demonstrated significant strain differences in ability to affect implantation of the fertilised ovum and to cause resorption of the mouse fetus. implantation was significantly impaired when c. fetus ss fetus was injected intravenously on day 2 of pregnancy, but no effect was observed in mice receiving c. jejuni. on day 6 of pregnancy, before the developme ...19882961890
clinical comparison of campylobacter jejuni and c coli diarrhoea. 19882893006
an outbreak of campylobacter jejuni infection in a neonatal intensive care unit. 19882895144
clinical characteristics of campylobacter jejuni and c coli enteritis. 19882895866
campylobacter jejuni infection as a hospital problem: an overview. 19882896736
in vitro susceptibility of diarrhoea producing gram negative enteric bacteria to sulfasalazine, 5-aminosalicylic acid, sulfapyridine and four quinolones. brief report.the in vitro susceptibility of diarrhoea producing gram negative enteric bacteria to sulfasalazine, 5-aminosalicylic acid, sulfapyridine and four quinolones was investigated using an agar dilution method. all strains were resistant to 1600 micrograms/ml of sulfasalazine and 5-aminosalicylic acid. mic range of sulfapyridine for y. enterocolitica was 3.1-25 micrograms/ml (median:6.2) and for salmonella 25-100 micrograms/ml (median: 100) campylobacter jejuni/coli were less susceptible to sulfapyrid ...19882899438
in vitro activity of azithromycin (cp-62,993), a novel macrolide, against enteric pathogens.azithromycin (cp-62,993 [9-deoxy-9a-methyl-9a-aza-9a-homoerythromycin]) is a novel macrolide antimicrobial. in this study the in vitro activity of cp-62,993 has been determined against selected enteropathogens, including clostridium difficile, and compared with that of erythromycin. mics were determined using an agar incorporation technique in mueller-hinton medium, containing saponin-lysed horse blood at a final concentration of 10% v/v, with an inoculum of 10(4) cfu. cp-62,993 was considerably ...19882854515
genomic rearrangements associated with antigenic variation in campylobacter coli.campylobacter coli and campylobacter jejuni share a limited number of highly conserved dna sequences with members of the family enterobacteriaceae. one of these sequences was cloned from c. coli vc167, and the region of homology to the enteric sequences was determined to be confined to a 700-base-pair region. the dna represented in this clone undergoes a programmed, reversible rearrangement in vc167 that is associated with flagellar antigenic variation.19882826396
[susceptibilities of clinical isolates to antibacterial agents. focusing mainly on ofloxacin (first report). reported by the research group for testing ofloxacin susceptibility of clinical isolates].susceptibility tests were carried out on a variety of clinically isolated pathogens using the susceptibility disc method at 197 hospitals in japan between may, 1985 through march, 1986. these tests were organized by the research group for testing ofloxacin susceptibility on clinical isolates, and the results were statistically analyzed. this paper describes a comparison of susceptibilities of clinical isolates including staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, st ...19883163383
isolation of campylobacter jejuni from quails: an initial report. 19883167556
[serologic diagnosis of campylobacteriosis. determination of antibodies to the outer membrane protein of campylobacter jejuni by the elisa method]. 19883167988
peroxide sensitivity and catalase activity in campylobacter jejuni after injury and during recovery.cells of campylobacter jejuni damaged by freezing or mild heat showed increased sensitivity to hydrogen peroxide and also required the addition of catalase for growth on nutrient agar. these phenomena, which could have important practical consequences, were not associated, however, with changes in total catalase activity and may be due to alterations in outer membrane permeability.19883170383
a specific dna probe for the identification of campylobacter jejuni.a 6.1 kb dna probe for the human enteric pathogen campylobacter jejuni has been isolated from a genomic library constructed in the plasmid vector pbr322 in escherichia coli. the dna sequence used as a probe was identified from recombinant plasmids following immunological screening of transformants using polyclonal antisera to whole cells and to membrane antigens of c. jejuni. restriction endonuclease fragment mapping of c. jejuni dna inserts from three of the recombinant plasmids showed an overl ...19883171548
isolation of a gastric campylobacter-like organism from the stomach of four rhesus monkeys, and identification as campylobacter pylori.campylobacter-like organisms, isolated from the gastric antrum of rhesus monkeys, were compared with campylobacter jejuni and c. pylori. they were similar to c. pylori by light microscopy, in ultrastructural morphology, in enzymic, fatty-acid-methyl-ester, and protein-profile analysis, and in antigenic reactivity with rabbit antisera to c. jejuni and c. pylori and with c. pylori-specific monoclonal antibody. because this natural infection of the rhesus monkey is associated with chronic gastritis ...19883172170
[laboratory and clinical studies of rokitamycin dry syrup in the field of pediatrics].laboratory and clinical studies on rokitamycin (rkm) dry syrup, a new macrolide antibiotic, were carried out in the field of pediatrics. the results are summarized as follows. 1. plasma concentrations and urinary recovery rates after oral administration on fasting of rkm dry syrup at doses of 10 mg/kg and 20 mg/kg to 2 and 1 cases, respectively, were determined. peak plasma levels were obtained in 30 minutes after administration of both dosages with half-lives of 1.5 to 2.2 hours. a clear-cut do ...19883172461
campylobacters in man and the environment in hull and east yorkshire.campylobacter organisms isolated from water samples taken weekly from ponds and land-drains in the city of hull were compared with isolates from humans. of 314 campylobacter organisms isolated from patients, 237 (75.5%) of the strains were identified as typical campylobacter jejuni, whilst of 125 identified strains isolated from the water samples, 85 (68%) resembled c. jejuni in most respects but were hippurate hydrolysis negative by the hwang and ederer method. the ponds and land drains in the ...19883181312
the pathogenicity of environmental campylobacters--a human volunteer experiment.three human volunteer experiments were performed in which river water expected to contain campylobacter organisms was ingested. despite the ingestion of over 44,000 organisms in one experiment, the subject did not suffer any symptoms, nor were campylobacter organisms excreted, nor was an antibody response to the ingested strains detected. the campylobacter organisms ingested resembled campylobacter jejuni on colonial and microscopic morphology but were hippurate negative, and were distinct from ...19883181313
the comparative in-vitro activity of ofloxacin.the antibacterial activity of ofloxacin, a new fluoroquinolone, was evaluated against a wide range of clinical bacterial isolates and compared with that of nalidixic acid, norfloxacin, enoxacin, pefloxacin and ciprofloxacin by determination of minimum inhibitory concentrations (mics). ofloxacin was very active against nalidixic acid-susceptible isolates of the enterobacteriaceae (mic less than or equal to 0.12 mg/l) and was also active against strains resistant to nalidixic acid (mic less than o ...19883182468
isolation of campylobacter jejuni from the bile of a cholecystic patient.described is the isolation of campylobacter jejuni from the bile of a cholecystic patient admitted for abdominal pain to an intensive care unit. a cholecystectomy was performed, and the patient responded to erythromycin therapy. details of this uncommon isolation are given.19883183005
an outbreak of food-borne campylobacter jejuni infection and the possible role of cross-contamination.a point-source outbreak or campylobacter infection affected 24 of 51 delegates attending a business lunch. failure to detect any specific vehicle for the infection led to observations of kitchen practice. experiments demonstrated that cross-contamination as a result of handling raw and cooked food consecutively was a possible cause of the outbreak.19883183409
methemoglobinemia and hemolytic anemia associated with campylobacter jejuni enteritis.a 7-week-old infant with methemoglobinemia, hemolytic anemia, and inadequate weight gain was found to have a campylobacter jejuni gastrointestinal tract infection. known etiologies of methemoglobinemia such as oxidative drug exposure, deficiency of nadh-methemoglobin reductase, and hemoglobin m disorder were excluded. the patient had a twin brother (probably identical) who had neither methemoglobinemia nor stool cultures positive for c. jejuni. the twin essentially served as an experimental cont ...19883189715
detection of two different kanamycin resistance genes in naturally occurring isolates of campylobacter jejuni and campylobacter coli.a total of 225 isolates of campylobacter jejuni and 54 isolates of campylobacter coli were screened for resistance to kanamycin. among these, five resistant isolates of c. jejuni and six resistant isolates of c. coli, all with different plasmid patterns, were identified. each contained at least one plasmid greater than or equal to 41 kilobases in size. the mic of kanamycin for all 11 strains was determined to be greater than or equal to 256 micrograms/ml by an agar dilution method. in addition, ...19883190204
is enrichment culture necessary for isolating campylobacter jejuni from faeces? 19883192739
incidence of campylobacter jejuni in processed egg products.egg samples were obtained from two commercial egg-processing facilities to determine if campylobacter jejuni could be isolated from the raw product or from further-processed egg products intended for human consumption. no c. jejuni were detected in any of the egg or egg-product samples or from water samples collected from the overflow of the egg washer. campylobacter jejuni was recovered from all control samples, which had been inoculated at the plants. these findings suggest that properly proce ...19883194336
in vitro activities of pd 117,596 and reference antibiotics against 448 clinical bacterial strains.the in vitro activity of pd 117,596, a new fluoroquinolone antibiotic, was tested against 448 bacterial isolates (15 genera) by agar dilution (inoculum, 10(4) cfu per spot). the activity of pd 117,596 was compared with that of 15 antibiotics against 327 gram-negative strains and with that of 8 other antibiotics against 121 gram-positive strains. pd 117,596 demonstrated the best activity against klebsiella spp., enterobacter spp., acinetobacter spp., serratia marcescens, and branhamella catarrhal ...19883196008
selective association and transport of campylobacter jejuni through m cells of rabbit peyer's patches.m cells in the peyer's patches may facilitate transport of pathogens such as campylobacter jejuni from the intestine. we evaluated this hypothesis by using electron microscopy to examine peyer's patches in ligated adult rabbit ileal loops inoculated with 5-ml suspensions of 10(9) cfu/ml of campylobacter jejuni. peyer's patches taken at intervals from 15 min to 2 h after inoculation of loops in anaesthetized rabbits provided evidence that campylobacter jejuni selectively adhered to m cells as opp ...19883196964
the occurrence of campylobacter jejuni in raw cows' milk.faeces and raw milk from individual cows were examined for the presence of campylobacter jejuni. after drawing milk, the lactoperoxidase system was inactivated by raising the ph to 7.5. the organism was isolated from 22% of 904 faecal samples and from 4.5% of 904 milk samples. from laboratory experiments it could be concluded that inactivation of the lactoperoxidase system resulted in a better isolation of c. jejuni from raw cows' milk.19883204072
[prevalence of campylobacter jejuni/coli in personnel and animals of the zoo park of santiago. analysis of the pathogenic factors]. 19883205994
yield of stool cultures, ova and parasite tests, and clostridium difficile determinations in nosocomial diarrheas.stool cultures for bacterial pathogens, ova and parasites, and clostridium difficile are usually ordered for patients with nosocomial diarrhea. in the interest of cost containment we undertook an 18-month retrospective study to assess the cost/benefit of performing each of these three tests. during the study period nosocomial diarrhea developed in 118 patients. of 452 bacterial stool cultures ordered, only one was positive for campylobacter jejuni and none for ova and parasites. however, of 126 ...19883207206
antimicrobial sensitivity and plasmid-mediated tetracycline resistance in campylobacter jejuni isolated in bangladesh.the antimicrobial sensitivity of campylobacter jejuni isolated in bangladesh from patients with diarrhoea, asymptomatic carriers and domestic animals was performed. all isolates were sensitive to erythromycin, gentamicin, furazolidone and kanamycin. seven percent isolates were resistant to tetracycline, 8% to nalidixic acid, 37% to ampicillin and 100% to sulfamethoxazole-trimethoprim and cephalothin. tetracycline resistance was observed to be plasmid mediated. no plasmid band(s) coding for ampic ...19883208549
antimicrobial susceptibility of campylobacter jejuni, campylobacter coli, and campylobacter laridis from cats, dogs, pigs, and seagulls. 19883210481
[effect of water activity on the growth of campylobacter jejuni in brain-heart infusion]. 19883212356
surface charge and hydrophobicity of campylobacter jejuni strains in relation to adhesion to epithelial ht-29 cells.hydrophobicity and surface charge of clinical isolates of campylobacter jejuni strains were investigated by aqueous two-phase partitioning (one-step and counter-current distribution), ion exchange chromatography and hydrophobic interaction chromatography. there was a good correlation between the different physico-chemical methods reflecting the same bacterial property. all strains were negatively charged and exposed a hydrophobic surface, but to a varying extent. bacteria with a high negative su ...19883214584
effect of incubation temperature, ageing, and bisulfite content of unsupplemented brucella agar on aerotolerance of campylobacter jejuni.a mutant strain of campylobacter jejuni atcc 29428 was isolated that grows on unsupplemented brucella agar at o2 levels as high as 21% at 37 degrees c. while measuring the degree of aerotolerance of this mutant on unsupplemented brucella medium and comparing it with that of the wild type, we found considerable variation among our estimates. as measured by colony counts on unsupplemented brucella agar incubated at various oxygen levels, the degree of aerotolerance was affected by incubation tempe ...19883214811
[which are the most frequently occurring enteropathogenic bacteria in clinical practice?].diarrhea is an increasingly frequent clinical finding in swiss medical practice. one reason is the volume of tourism in countries with different climatic and social circumstances. to obtain an overview of the enteropathogenic bacteria spectrum as a cause of diarrhea, we analyzed 11,163 stool specimens from a representative group of patients throughout switzerland. enteropathogenic bacteria were found in 15.2% of all specimens; 50% were campylobacter jejuni/coli, 35% salmonella spp., 8% aeromonas ...19883217775
survival of coliforms and bacterial pathogens within protozoa during chlorination.the susceptibility of coliform bacteria and bacterial pathogens to free chlorine residuals was determined before and after incubation with amoebae and ciliate protozoa. viability of bacteria was quantified to determine their resistance to free chlorine residuals when ingested by laboratory strains of acanthamoeba castellanii and tetrahymena pyriformis. cocultures of bacteria and protozoa were incubated to facilitate ingestion of the bacteria and then were chlorinated, neutralized, and sonicated ...19883223766
antibacterial antibody pattern in seronegative spondyloarthropathies (snsa).the occurrence of some anti-bacterial antibodies was studied in sera from 31 healthy donors (hd) and 101 patients with different rheumatic diseases. the cases investigated included 7 psoriatic arthritis (pa), 35 rheumatoid arthritis (ra), 17 undifferentiated seronegative spondyloarthritis (u-snsa), 13 behçet's syndrome, 18 enteric arthropathies (ea), 7 ankylosing spondylitis (as) and 4 reiter's syndrome. a complement fixation test was carried out to detect the presence and to evaluate the titer ...19883229028
role of the 92.5-kilodalton outer membrane protein of campylobacter jejuni in serological reactions.outer membrane proteins from 12 strains of campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with rabbit antisera prepared against formalin-treated cell suspensions of three of the strains. the 92.5-kilodalton protein was confirmed to be a minor outer membrane protein and exhibited serological heterogeneity. serological specificities of the proteins, however, were found not to correlate with serotypic specificities of the strains, ...19883230126
incidence and level of campylobacter jejuni in broiler processing.the distribution and level of campylobacter jejuni throughout broiler processing was evaluated. six trials were conducted at three broiler processing plants to determine the occurrence of c. jejuni on commercial broilers as affected by specific processing functions. counts were determined at specific sampling stations throughout the plants, and patterns of counts among sampling stations and plants were observed. between 1,100 and 5,500 c. jejuni/1,000 cm2 skin surface (represents half the skin s ...19883237574
[survival of campylobacter jejuni in brain and heart extracts of different ph]. 19883238294
comparative in vitro potencies of nine new macrolides.the in vitro activities of the 14-membered macrolides (14m) clarithromycin (cl), roxithromycin (ro), dirithromycin (di) and flurithromycin (fl), a 15-membered macrolide (15m) azithromycin (az), and the 16-membered macrolides (16m) josamycin (jm), spiramycin (sp), miocamycin (mi) and rokitamycin (rk) were compared with erythromycin (er). cl was two- to four-fold more potent than er against all organisms except haemophilus and campylobacter jejuni against which it was two-fold less active. ro and ...19883240705
an outbreak of campylobacter jejuni gastroenteritis at a resort--ontario. 19883242891
effect of mucin on campylobacter jejuni association and invasion on hep-2 cells.we report the development of an in vitro assay using hep-2 cell monolayers in which cell association of five c. jejuni strains and e. coli k12 c600 were tested under different experimental conditions. both total cell associated colony forming units (cfu) and the cfu protected from gentamicin killing (i.e. internalized bacteria) were determined. pretreatment of hep-2 cells with mucin (100 micrograms/ml) enhanced internalization of four fresh isolates of c. jejuni by 3.2 to 20.7-fold, depending on ...19883244310
antibodies against campylobacter jejuni/coli in patients suffering from campylobacteriosis or inflammatory bowel disease.sera from 74 patients from the oslo area and 21 from northern norway with compylobacteriosis were analysed for antibodies against an antigen mixture comprising seven campylobacter jejuni/coli strains including a pen 0:6,7 isolate representing the most common serotype in northern norway. seventy-seven per cent of the campylobacter patients from the oslo area and 86% from northern norway had elevated antibody levels. thirty-seven out of 57 (65%) seropositive patients from oslo and 12 out of 18 (67 ...19883247063
acute diarrhoeal disease in nigeria: detection of enteropathogens in a rural sub-saharan population.a retrospective study was carried out to obtain epidemiological data concerning the relative risk of shigella, salmonella, campylobacter jejuni and vibrio cholerae infection in several rural population groups. faecal samples (rectal swab specimens) were cultured from 914 selected children with acute diarrhoeal disease and from a matched control group of 984 non-diarrhoeal children. shigella were more frequently isolated than the other enteropathogens from both the diarrhoeal (12.7%) and control ...19883252601
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