Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| evaluation of the incidence of periodontitis-associated bacteria in the atherosclerotic plaque of coronary blood vessels. | unstable atherosclerotic plaque is a dangerous clinical condition, possibly leading to acute coronary deficiency resulting in cardiac infarction. questions about the role of inflammatory factors in the formation of pathological lesions in the endothelium of coronary vessels have often been raised. this condition may be caused by bacteria that are able to initiate clot formation in a blood vessel, destabilizing an atherosclerotic plaque that is already present. the sources of these pathogens are ... | 2007 | 17274722 |
| genome of the opportunistic pathogen streptococcus sanguinis. | the genome of streptococcus sanguinis is a circular dna molecule consisting of 2,388,435 bp and is 177 to 590 kb larger than the other 21 streptococcal genomes that have been sequenced. the g+c content of the s. sanguinis genome is 43.4%, which is considerably higher than the g+c contents of other streptococci. the genome encodes 2,274 predicted proteins, 61 trnas, and four rrna operons. a 70-kb region encoding pathways for vitamin b(12) biosynthesis and degradation of ethanolamine and propanedi ... | 2007 | 17277061 |
| intermolecular forces and enthalpies in the adhesion of streptococcus mutans and an antigen i/ii-deficient mutant to laminin films. | the antigen i/ii family of surface proteins is expressed by most oral streptococci, including streptococcus mutans, and mediates specific adhesion to, among other things, salivary films and extracellular matrix proteins. in this study we showed that antigen i/ii-deficient s. mutans isogenic mutant ib03987 was nearly unable to adhere to laminin films under flow conditions due to a lack of specific interactions (0.8 x 10(6) and 1.1 x 10(6) cells cm(-2) at ph 5.8 and 6.8, respectively) compared wit ... | 2007 | 17277062 |
| physiological osmotic induction of leptospira interrogans adhesion: liga and ligb bind extracellular matrix proteins and fibrinogen. | transmission of leptospirosis occurs through contact of mucous membranes and abraded skin with freshwater contaminated by pathogenic leptospira spp. exposure to physiological osmolarity induces leptospires to express high levels of the lig surface proteins containing imperfect immunoglobulin-like repeats that are shared or differ between liga and ligb. we report that osmotic induction of lig is accompanied by 1.6- to 2.5-fold increases in leptospiral adhesion to immobilized extracellular matrix ... | 2007 | 17296754 |
| novel tetracycline resistance determinant isolated from an environmental strain of serratia marcescens. | resistances to tetracycline and mercury were identified in an environmental strain of serratia marcescens isolated from a stream highly contaminated with heavy metals. as a step toward addressing the mechanisms of coselection of heavy metal and antibiotic resistances, the tetracycline resistance determinant was cloned in escherichia coli. within the cloned 13-kb segment, the tetracycline resistance locus was localized by deletion analysis and transposon mutagenesis. dna sequence analysis of an 8 ... | 2007 | 17308196 |
| microbiological outcomes of quadrant versus full-mouth root planing as monitored by real-time pcr. | to study the short-term microbiological changes following full-mouth compared with quadrant wise scaling and root planing (fmrp and qrp) as well as long-term effects. | 2007 | 17309590 |
| subgingival plaque microbiota in hiv positive patients. | to describe and compare the predominant bacterial and fungal species associated with gingivitis, periodontitis, and linear gingival erythema (lge), in hiv positive subjects with different immune status. | 2007 | 17309593 |
| a comparison of implant-supported, bar- or ball-retained mandibular overdentures: a retrospective clinical, microbiologic, and immunologic study of 10 edentulous patients attending a recall visit. | clinical, microbiologic, and immunologic comparisons of the peri-implant health in edentulous volunteers wearing long-standing implant-supported ball- or dolder bar-retained mandibular overdentures were performed. | 2007 | 17319360 |
| mechanisms of decreased susceptibility to beta-defensins by treponema denticola. | treponema denticola, a periodontal pathogen, is relatively resistant to human beta-defensins, which are small cationic antimicrobial peptides produced by a number of cells, including the gingival epithelium. using two independent methods, we previously demonstrated that t. denticola proteases are not responsible for decreased vulnerability to defensins. in this study, we confirmed that the major outer membrane protease, dentilisin, is not responsible for t. denticola insensitivity to defensins a ... | 2007 | 17325047 |
| subgingival chlorhexidine varnish administration as an adjunct to same-day full-mouth root planing. ii. microbiological observations. | recently, the clinical additive value of a highly concentrated chlorhexidine (chx) varnish, when applied subgingivally as an adjunct to scaling and root planing, was described. the objective of the present study was to investigate the microbiological impact of a treatment strategy for chronic periodontitis based on a combination of same-day full-mouth root planing and subgingival chx varnish administration. | 2007 | 17335366 |
| flow cytometric analysis of adherence of porphyromonas gingivalis to oral epithelial cells. | by using fluorescence microscopy, fluorescently labeled porphyromonas gingivalis w50 was shown to adhere to oral epithelial (kb) cells as discrete cells or small cell aggregates, whereas p. gingivalis atcc 33277 bound as large cell aggregates. flow cytometric analysis showed that for p. gingivalis w50 there was a logarithmic relationship between the bacterial cell ratio (bcr), that is the number of bacterial cells to kb cells, and the percentage of kb cells with w50 cells attached. this percenta ... | 2007 | 17339349 |
| activation of mapk in fibroblasts by treponema denticola major outer sheath protein. | the major outer sheath protein (msp) of treponema denticola induces ca(2+) entry and actin reorganization in cultured fibroblasts, but the pathways by which msp mediates these responses are not yet defined. we considered that msp may activate protein kinases as a stress response that precedes actin remodelling. phospho-kinase screens showed that msp induced phosphorylation of multiple kinases in pathways that respond to extracellular agonists and regulate actin assembly. 34 kinases were signific ... | 2007 | 17346673 |
| the detection of eight putative periodontal pathogens in adult and rapidly progressive periodontitis patients: an institutional study. | periodontal disease is a commonly prevalent problem faced alike by both the developed and third world countries but showing wide variations in prevalence and severity across different geographical areas. the purpose was to identify actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), prevotella intermedia (pi), ekinella corrodens (ec), campylobacter rectus (cr), bacteroides forsythus (bf), treponema denticola (td) and fusobacterium nucleatum (fn) in indian adult periodontiti ... | 2007 | 17347537 |
| a novel prokaryotic trans-2-enoyl-coa reductase from the spirochete treponema denticola. | an nadh-dependent trans-2-enoyl-coa reductase (ec1.1.1.36) from the gram negative spirochete treponema denticola was identified, expressed and biochemically characterized. the recombinant protein is a monomeric enzyme with a molecular mass of 44 kda with a specific activity of 43+/-4.8 u/mg (micromol mg(-1)min(-1)) and k(m) value of 2.7 microm for crotonoyl-coa. this nadh-dependent trans-2-enoyl-coa reductase represents the first enzymatically characterized member of a prokaryotic protein family ... | 2007 | 17382934 |
| evidence of periopathogenic microorganisms in placentas of women with preeclampsia. | periodontal disease is a chronic inflammatory infectious disease that may act as a focus of infection. preeclampsia is a pregnancy-specific hypertensive disorder that often leads to maternal morbidity and mortality. acute atherosis, the placental lesion of preeclampsia, shares many histopathological features with atherosclerosis. recently, chronic infection was linked to the initiation of atherosclerosis. oral pathogens have been detected in atherosclerotic plaques, where they may play a role in ... | 2007 | 17397314 |
| prevalence of four putative periodontopathic bacteria in saliva of a group of brazilian children with mixed dentition: 1-year longitudinal study. | this study aimed at evaluating the prevalence of putative periodontal pathogens (actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella nigrescens, treponema denticola) in saliva of children with mixed dentition at two different time points, correlating these findings with a clinical parameter of gingival health. | 2007 | 17397463 |
| intrinsic apoptotic pathways of gingival epithelial cells modulated by porphyromonas gingivalis. | porphyromonas gingivalis can inhibit chemically induced apoptosis in primary cultures of gingival epithelial cells through blocking activation of the effector caspase-3. the anti-apoptotic phenotype of p. gingivalis is conserved across strains and does not depend on the presence of fimbriae, as fimbriae-deficient mutants and a naturally occurring non-fimbriated strain were able to impede apoptosis. to dissect the survival pathways modulated by p. gingivalis, protein and gene expression of a numb ... | 2007 | 17419719 |
| aggregatibacter actinomycetemcomitans as indicator for aggressive periodontitis by two analysing strategies. | to compare the subgingival microbiota of aggressive and chronic periodontitis (chp) using single-site and pooled plaque samples. | 2007 | 17433043 |
| performance of captia selectsyph-g enzyme-linked immunosorbent assay in syphilis testing of a high-risk population: analysis of discordant results. | using the captia select syph-g enzyme-linked immunosorbent assay (elisa), we analyzed the sera of 1,771 patients from a high-risk population attending sexually transmitted disease (std) clinics. we focused on discrepancies between the results of the immunoglobulin g (igg) elisa and the treponema pallidum hemagglutination (tpha) test. we identified 57 patients (3.22%) with conflicting results in the igg elisa and tpha test. in order to resolve these discrepancies, these patients' health records w ... | 2007 | 17442795 |
| identification of intracellular oral species within human crevicular epithelial cells from subjects with chronic periodontitis by fluorescence in situ hybridization. | interactions between oral bacteria and gingival epithelial cells play an important role in the pathogenesis of periodontal diseases. this study used in situ hybridization with 16 rrna probes and confocal microscopy to detect the periodontal pathogens porphyromonas gingivalis, actinobacillus actinomycetemcomitans, tannerella forsythia, and treponema denticola within epithelial cells from periodontal pockets, gingival crevice, and buccal mucosa collected from subjects with chronic periodontitis (n ... | 2007 | 17451543 |
| periodontal pathogens in periodontal pockets and in carotid atheromatous plaques. | in the last years the relationship between periodontitis and cardiovascular diseases has been a subject of increasing research. the identification of periodontopathic bacteria in atheromas can contribute to our knowledge of such an association. the aim of our study was to assess the concomitant presence of 5 periodontal pathogens (actinobacillus actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, treponema denticola, and tannerella forsythia) in periodontal pockets and in car ... | 2007 | 17452955 |
| characterization of the thermotoga maritima chemotaxis methylation system that lacks pentapeptide-dependent methyltransferase cher:mcp tethering. | sensory adaptation in bacterial chemotaxis is mediated by covalent modifications of specific glutamate and glutamine residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins (mcps). in escherichia coli and salmonella enterica, efficient methylation of mcps depends on the localization of methyltransferase cher to mcp clusters through an interaction between the cher beta-subdomain and a pentapeptide sequence (nwetf or nwesf) at the c-terminus of the mcp. in vitro methylation ... | 2007 | 17163981 |
| characterization of the thermotoga maritima chemotaxis methylation system that lacks pentapeptide-dependent methyltransferase cher:mcp tethering. | sensory adaptation in bacterial chemotaxis is mediated by covalent modifications of specific glutamate and glutamine residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins (mcps). in escherichia coli and salmonella enterica, efficient methylation of mcps depends on the localization of methyltransferase cher to mcp clusters through an interaction between the cher beta-subdomain and a pentapeptide sequence (nwetf or nwesf) at the c-terminus of the mcp. in vitro methylation ... | 2007 | 17163981 |
| antibodies to periodontal organisms are associated with decreased kidney function. the dental atherosclerosis risk in communities study. | increasing evidence suggests that clinical signs of periodontal disease are independently associated with renal impairment. however, no studies have examined the possible linkage of kidney disease with serum antibody to oral pathogens. | 2007 | 17170550 |
| identification of specific chemoattractants and genetic complementation of a borrelia burgdorferi chemotaxis mutant: flow cytometry-based capillary tube chemotaxis assay. | measuring the chemotactic response of borrelia burgdorferi, the bacterial species that causes lyme disease, is relatively more difficult than measuring that of other bacteria. because these spirochetes have long generation times, enumerating cells that swim up a capillary tube containing an attractant by using colony counts is impractical. furthermore, direct counts with a petroff-hausser chamber is problematic, as this method has a low throughput and necessitates a high cell density; the latter ... | 2007 | 17172459 |
| identification of specific chemoattractants and genetic complementation of a borrelia burgdorferi chemotaxis mutant: flow cytometry-based capillary tube chemotaxis assay. | measuring the chemotactic response of borrelia burgdorferi, the bacterial species that causes lyme disease, is relatively more difficult than measuring that of other bacteria. because these spirochetes have long generation times, enumerating cells that swim up a capillary tube containing an attractant by using colony counts is impractical. furthermore, direct counts with a petroff-hausser chamber is problematic, as this method has a low throughput and necessitates a high cell density; the latter ... | 2007 | 17172459 |
| phylogenetic analysis of bacterial and archaeal species in symptomatic and asymptomatic endodontic infections. | phylogenetic analysis of bacterial and archaeal 16s rrna was used to examine polymicrobial communities within infected root canals of 20 symptomatic and 14 asymptomatic patients. nucleotide sequences from approximately 750 clones amplified from each patient group with universal bacterial primers were matched to the ribosomal database project ii database. phylotypes from 37 genera representing actinobacteria, bacteroidetes, firmicutes, fusobacteria and proteobacteria were identified. results were ... | 2007 | 17172525 |
| molecular analysis of the subgingival microbiota in health and disease. | this investigation provides molecular analyses of the periodontal microbiota in health and disease. subgingival samples from 47 volunteers with healthy gingivae or clinically diagnosed chronic periodontitis were characterized by pcr-denaturing gradient gel electrophoresis (dgge) with primers specific for the v2-v3 region of the eubacterial 16s rrna gene. a hierarchical dendrogram was constructed from band patterns. all unique pcr amplicons (dgge bands) were sequenced for identity. samples were a ... | 2007 | 17085691 |
| molecular analysis of the subgingival microbiota in health and disease. | this investigation provides molecular analyses of the periodontal microbiota in health and disease. subgingival samples from 47 volunteers with healthy gingivae or clinically diagnosed chronic periodontitis were characterized by pcr-denaturing gradient gel electrophoresis (dgge) with primers specific for the v2-v3 region of the eubacterial 16s rrna gene. a hierarchical dendrogram was constructed from band patterns. all unique pcr amplicons (dgge bands) were sequenced for identity. samples were a ... | 2007 | 17085691 |
| comparison of a taqman-based real-time polymerase chain reaction with conventional tests for the detection of trichomonas vaginalis. | to compare a taqman-based real-time polymerase chain reaction (pcr) with conventional pcr, culture, and wet-mount microscopy for the diagnosis of trichomoniasis in women. | 2007 | 17090567 |
| comparison of a taqman-based real-time polymerase chain reaction with conventional tests for the detection of trichomonas vaginalis. | to compare a taqman-based real-time polymerase chain reaction (pcr) with conventional pcr, culture, and wet-mount microscopy for the diagnosis of trichomoniasis in women. | 2007 | 17090567 |
| identification of the gene encoding the fhbb protein of treponema denticola, a highly unique factor h-like protein 1 binding protein. | the gene encoding the treponema denticola factor h-like protein 1 (fhl-1) binding protein, fhbb, was recovered and characterized. sequence conservation, expression, and properties of fhbb were analyzed. the identification of fhbb represents an important step in understanding the contribution of fhl-1 binding in t. denticola pathogenesis and in development of periodontal disease. | 2007 | 17101650 |
| rmlc, a c3' and c5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation. | the striking feature of carbohydrates is their constitutional, conformational and configurational diversity. biology has harnessed this diversity and manipulates carbohydrate residues in a variety of ways, one of which is epimerization. rmlc catalyzes the epimerization of the c3' and c5' positions of dtdp-6-deoxy-d-xylo-4-hexulose, forming dtdp-6-deoxy-l-lyxo-4-hexulose. rmlc is the third enzyme of the rhamnose pathway, and represents a validated anti-bacterial drug target. although several stru ... | 2007 | 17046787 |
| rmlc, a c3' and c5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation. | the striking feature of carbohydrates is their constitutional, conformational and configurational diversity. biology has harnessed this diversity and manipulates carbohydrate residues in a variety of ways, one of which is epimerization. rmlc catalyzes the epimerization of the c3' and c5' positions of dtdp-6-deoxy-d-xylo-4-hexulose, forming dtdp-6-deoxy-l-lyxo-4-hexulose. rmlc is the third enzyme of the rhamnose pathway, and represents a validated anti-bacterial drug target. although several stru ... | 2007 | 17046787 |
| two subgingival plaque-sampling strategies used with rna probes. | the purpose of this study was to compare the results of microbiological rna-probe analysis after subgingival plaque sampling applying two different strategies. | 2007 | 17199542 |
| rat model of polymicrobial infection, immunity, and alveolar bone resorption in periodontal disease. | one of the predominant polymicrobial infections of humans is expressed clinically as periodontal disease. porphyromonas gingivalis, treponema denticola, and tannerella forsythia have been strongly implicated as members of a pathogenic consortium in the etiology of adult periodontitis. in this study we hypothesized that p. gingivalis, t. denticola, and t. forsythia are synergistic in terms of virulence potential and induce chronic periodontal inflammation that leads to alveolar bone resorption in ... | 2007 | 17210663 |
| fatty acids synthesized by oral treponemes in chemically defined media. | omiz-w68, a chemically defined medium that contains no long-chain fatty acids and yet supports in vitro proliferation of a wide range of fastidious oral anaerobes, is described. the type strains of treponema denticola, treponema lecithinolyticum, treponema maltophilum, treponema pectinovorum, treponema socranskii, and an as yet unpublished canine treponema species could be propagated indefinitely in this medium with sugar supplements for the saccharolytic species. analysis of the cellular fatty ... | 2007 | 17229060 |
| characterization of treponema denticola pyrf encoding orotidine-5'-monophosphate decarboxylase. | the treponema denticola atcc 35405 genome annotation contains most of the genes for de novo pyrimidine biosynthesis. to initiate characterization of pyrimidine synthesis in treponema, we focused on tde2110 (the putative pyrf, encoding orotidine-5'-monophosphate decarboxlyase). unlike the parent strain, an isogenic pyrf mutant was resistant to 5-fluoroorotic acid. in complex medium, growth of the pyrf mutant was independent of added uracil, indicating activity of a uracil uptake/salvage pathway. ... | 2007 | 17187656 |
| [pathogenic potential of porphyromonas gingivalis, treponema denticola and tannerella forsythia, the red bacterial complex associated with periodontitis]. | periodontitis are mixed bacterial infections leading to destruction of tooth-supporting tissues, including periodontal ligament and alveolar bone. among over 500 bacterial species living in the oral cavity, a bacterial complex named "red complex" and made of porphyromonas gingivalis, treponema denticola and tannerella forsythia has been strongly related to advanced periodontal lesions. while periodontopathogenic bacteria are the primary etiologic factor of periodontitis, tissue destruction essen ... | 2007 | 17049750 |
| assessment of cell-surface exposure and vaccinogenic potentials of treponema pallidum candidate outer membrane proteins. | syphilis, a sexually transmitted infection caused by the spirochetal bacterium treponema pallidum, remains a global public health problem. t. pallidum is believed to be an extracellular pathogen and, as such, the identification of t. pallidum outer membrane proteins that could serve as targets for opsonic or bactericidal antibodies has remained a high research priority for vaccine development. however, the identification of t. pallidum outer membrane proteins has remained highly elusive. recent ... | 2007 | 17890130 |
| benefits of additional courses of systemic azithromycin in periodontal disease case report. | the strong association of subgingival anaerobic bacteria, such as porphyromonas gingivalis, treponema denticola and tannerella forsythia, with destructive periodontal disease has been well documented in the literature. several double-blind studies have also shown the beneficial use of systemic antimicrobials that are active against these microorganisms in conjunction with conventional periodontal treatment, especially when periodontal abscesses and/or suppuration upon probing are present. four c ... | 2007 | 17891880 |
| genome differences between treponema pallidum subsp. pallidum strain nichols and t. paraluiscuniculi strain cuniculi a. | the genome of treponema paraluiscuniculi strain cuniculi a was compared to the genome of the syphilis spirochete treponema pallidum subsp. pallidum strain nichols using dna microarray hybridization, whole-genome fingerprinting, and dna sequencing. a dna microarray of t. pallidum subsp. pallidum nichols containing all 1,039 predicted open reading frame pcr products was used to identify deletions and major sequence changes in the cuniculi a genome. using these approaches, deletions, insertions, an ... | 2007 | 17893135 |
| identification of a signalling molecule involved in bacterial intergeneric communication. | the development of complex multispecies communities such as biofilms is controlled by interbacterial communication systems. we have previously reported an intergeneric communication between two oral bacteria, streptococcus cristatus and porphyromonas gingivalis, that results in inhibition of fima expression. here, we demonstrate that a surface protein, arginine deiminase (arca), of s. cristatus serves as a signal that initiates intergeneric communication. an arca-deficient mutant of s. cristatus ... | 2007 | 17906122 |
| implant surface analysis and microbiologic evaluation of failed implants retrieved from smokers. | the aim of this study was to evaluate the microbiota and surface of failed titanium dental implants from 4 manufacturers. twelve mobile dental implants were retrieved from 10 smokers after 3 to 10 years of functional loading. before implant removal, microbial samples were taken and evaluated using polymerase chain reaction. after implant removal, analyses of the failed implant surfaces were performed using scanning electron microscopy and energy-dispersive spectrometer x-ray. periodontal pathoge ... | 2007 | 17912965 |
| effect of adjunctive systemic azithromycin with periodontal surgery in the treatment of chronic periodontitis in smokers: a pilot study. | along with conventional surgical therapy, systemic antibiotics may provide more effective treatment in smokers by targeting tissue-invasive bacteria. the aim of this randomized, placebo-controlled, double-masked clinical trial was to evaluate the adjunctive effects of systemic azithromycin (azm) in combination with periodontal pocket reduction surgery in the treatment of chronic periodontitis in smokers. | 2007 | 17915999 |
| physiological ecology of stenoxybacter acetivorans, an obligate microaerophile in termite guts. | stenoxybacter acetivorans is a newly described, obligately microaerophilic beta-proteobacterium that is abundant in the acetate-rich hindgut of reticulitermes. here we tested the hypotheses that cells are located in the hypoxic, peripheral region of reticulitermes flavipes hindguts and use acetate to fuel their o(2)-consuming respiratory activity in situ. physical fractionation of r. flavipes guts, followed by limited-cycle pcr with s. acetivorans-specific 16s rrna gene primers, indicated that c ... | 2007 | 17827335 |
| delineation of species-specific binding properties of the cspz protein (bbh06) of lyme disease spirochetes: evidence for new contributions to the pathogenesis of borrelia spp. | borrelia burgdorferi cspz (tigr open reading frame designation, bbh06) is part of a functionally related group of proteins that bind one or more members of the factor h (fh) protein family. in this report we assess the conservation, distribution, properties, and ligand binding abilities of cspz from the three main borrelia species associated with lyme disease infections in humans. cspz (also referred to as bbcrasp-2 in the literature) was found to be highly conserved at the intraspecies level bu ... | 2007 | 17846117 |
| structural and biochemical basis for polyamine binding to the tp0655 lipoprotein of treponema pallidum: putative role for tp0655 (tppotd) as a polyamine receptor. | tp0655 of treponema pallidum, the causative agent of syphilis, is predicted to be a 40 kda membrane lipoprotein. previous sequence analysis of tp0655 noted its homology to polyamine-binding proteins of the bacterial potd family, which serve as periplasmic ligand-binding proteins of atp-binding-cassette (abc) transport systems. here, the 1.8 a crystal structure of tp0655 demonstrated structural homology to escherichia coli potd and potf. the latter two proteins preferentially bind spermidine and ... | 2007 | 17868688 |
| treponema denticola activates mitogen-activated protein kinase signal pathways through toll-like receptor 2. | treponema denticola, a spirochete indigenous to the oral cavity, is associated with host inflammatory responses to anaerobic polymicrobial infections of the root canal, periodontium, and alveolar bone. however, the cellular mechanisms responsible for the recognition of t. denticola by the innate immune system and the underlying cell signaling pathways that regulate the inflammatory response to t. denticola are currently unresolved. in this study, we demonstrate that t. denticola induces innate i ... | 2007 | 17923521 |
| polymerase chain reaction of porphyromonas gingivalis, treponema denticola, and tannerella forsythia in primary endodontic infections. | the aim of this study was to investigate the correlation between endodontic clinical signs and symptoms and the presence of porphyromonas gingivalis, treponema denticola, and tannerella forsythia or their association by nested polymerase chain reaction assay. microbial samples were taken from 50 cases with necrotic pulp tissues in primary infections. dna was extracted from the samples, which were analyzed for the presence of three endodontic pathogens by using species-specific primers. p gingiva ... | 2007 | 17931930 |
| structure of arabidopsis thaliana 5-methylthioribose kinase reveals a more occluded active site than its bacterial homolog. | metabolic variations exist between the methionine salvage pathway of humans and a number of plants and microbial pathogens. 5-methylthioribose (mtr) kinase is a key enzyme required for methionine salvage in plants and many bacteria. the absence of a mammalian homolog suggests that mtr kinase is a good target for the design of specific herbicides or antibiotics. | 2007 | 17961230 |
| identification of prophages in bacterial genomes by dinucleotide relative abundance difference. | prophages are integrated viral forms in bacterial genomes that have been found to contribute to interstrain genetic variability. many virulence-associated genes are reported to be prophage encoded. present computational methods to detect prophages are either by identifying possible essential proteins such as integrases or by an extension of this technique, which involves identifying a region containing proteins similar to those occurring in prophages. these methods suffer due to the problem of l ... | 2007 | 18030328 |
| the correlation between serum immunoglobulin a and immunoglobulin g levels and the presence of treponema denticola in human periapical lesions. | the aim of this study was to compare the serum immunoglobulin a (iga) and immunoglobulin g (igg) levels and the presence of treponema denticola in the root canals in a group of teeth with/without periapical lesion. a total of 66 children aged 8 to 13 years old were involved in this study. five milliliters of blood samples were taken to detect the serum iga and igg levels. sixty-six endodontic samplings were also obtained to determine the presence of t. denticola by polymerase chain reaction. the ... | 2007 | 18037048 |
| identification of actinobacillus actinomycetemcomitans, treponema denticola and porphyromonas gingivalis within human dental calculus: a pilot investigation. | dental calculus is considered to be simply a "plaque-retentive factor", and therefore only a secondary aetiological factor in the pathogenesis of periodontitis. recent studies have suggested a more active role for calculus. our objective was to demonstrate the presence of periodontal pathogens in the non-mineralised areas of supra- and subgingival dental calculus. | 2007 | 17987882 |
| interspecies interactions within oral microbial communities. | while reductionism has greatly advanced microbiology in the past 400 years, assembly of smaller pieces just could not explain the whole! modern microbiologists are learning "system thinking" and "holism." such an approach is changing our understanding of microbial physiology and our ability to diagnose/treat microbial infections. this review uses oral microbial communities as a focal point to describe this new trend. with the common name "dental plaque," oral microbial communities are some of th ... | 2007 | 18063722 |
| high incidence of actinobacillus actinomycetemcomitans infection in acute coronary syndrome. | recent epidemiological studies suggest that periodontitis is an important risk factor for coronary heart disease (chd). the aim of this study was to evaluate the association between periodontitis and chd, particularly acute coronary syndrome (acs), focusing on microbiological and immunological features. twenty-eight chd patients, 15 with acs and 13 with chronic chd, were included in this study. coronary angiography, periodontal examination, and dental radiography were performed in all patients. ... | 2007 | 18160759 |
| assessment of the microbiota in root canals of human primary teeth by checkerboard dna-dna hybridization. | the purpose of this study was to evaluate in vivo the prevalence of microorganisms in root canals of human primary teeth, by checkerboard dna-dna hybridization. | 2007 | 18477431 |
| the role of released atp in killing candida albicans and other extracellular microbial pathogens by cationic peptides. | a unifying theme common to the action of many cationic peptides that display lethal activities against microbial pathogens is their specific action at microbial membranes that results in selective loss of ions and small nucleotides-chiefly atp. one model cationic peptide that induces non-lytic release of atp from the fungal pathogen candida albicans is salivary histatin 5 (hst 5). the major characteristic of hst 5-induced atp release is that it occurs rapidly while cells are still metabolically ... | 2007 | 18404422 |
| antibody levels to single bacteria or in combination evaluated against myocardial infarction. | evidence is accumulating that oral bacteria are associated with myocardial infarctions (mi). we were interested in studying the differences in the association between single bacteria or bacteria in combination and the relation to c-reactive protein (crp). | 2008 | 18410396 |
| genome-wide subcellular localization of putative outer membrane and extracellular proteins in leptospira interrogans serovar lai genome using bioinformatics approaches. | in bacterial pathogens, both cell surface-exposed outer membrane proteins and proteins secreted into the extracellular environment play crucial roles in host-pathogen interaction and pathogenesis. considerable efforts have been made to identify outer membrane (om) and extracellular (ex) proteins produced by leptospira interrogans, which may be used as novel targets for the development of infection markers and leptospirosis vaccines. | 2008 | 18423054 |
| microbial analysis of canals of root-filled teeth with periapical lesions using polymerase chain reaction. | the objective of the present study was to investigate the presence of nine bacterial species in root-filled teeth associated with periapical lesions using a polymerase chain reaction analysis and to correlate these species with clinical features of the cases. dna was extracted from 45 canal samples of root-filled teeth with periapical lesions. a pcr assay using species-specific primers of 16s rdna and the downstream intergenic spacer region was used for microbial detection. enterococcus faecalis ... | 2008 | 18436030 |
| the prevalence of periodontopathogenic bacteria in saliva is linked to periodontal health status and oral malodour. | this study investigated whether an improvement in periodontal health resulted in changes in the prevalence of periodontopathogenic bacteria in saliva and tongue coatings and a reduction in volatile sulfur compounds (vscs: h(2)s and ch(3)sh) linked to oral malodour. the subjects were 35 patients who visited the breath odour clinic of kyushu dental college, japan. their mean age was 51.2+/-18.3 years (mean+/-sd). a clinical examination performed at baseline and 2 months after periodontal treatment ... | 2008 | 18436598 |
| detection of treponema denticola in root canal systems in primary and secondary endodontic infections. a correlation with clinical symptoms. | the aim of the study was to investigate the presence of treponema denticola in primary and secondary root-infected canal systems with periapical pathology and correlations with clinical signs and symptoms. | 2008 | 18437843 |
| a variable gene in a conserved region of the helicobacter pylori genome: isotopic gene replacement or rapid evolution? | the present study concerns the identification of a novel coding sequence in a region of the helicobacter pylori genome, located between jhp1069/hp1141 and jhp1071/hp1143 according to the numbering of the j99 and 26,695 reference strains, respectively, and spanning three different coding dna sequences (cdss). the cdss located at the centre of this locus were highly polymorphic, as determined by the analysis of 24 european isolates, 3 asian, and 3 african isolates. phylogenetic and molecular evolu ... | 2008 | 18442984 |
| comparative analysis of putative periodontopathic bacteria by multiplex polymerase chain reaction. | the polymerase chain reaction (pcr) has been applied for the rapid and specific detection of periodontopathic bacteria in subgingival plaque and is potentially of clinical benefit in the diagnosis and treatment of periodontitis subjects. however, several technical points need to be modified before the conventional pcr detection system can be used by clinicians. | 2008 | 18447853 |
| effects of age and oral disease on systemic inflammatory and immune parameters in nonhuman primates. | this report evaluated systemic inflammatory and immune biomarkers in a cohort of macaca mulatta (rhesus monkeys) maintained as a large family social unit, including an age range from <1 year to >24 years. we hypothesized that the systemic host responses would be affected by the age, gender, and clinical oral presentation of the population, each contributing to inflammatory and immune responses that would reflect chronic oral infections. the results demonstrated that the prevalence and severity o ... | 2008 | 18448617 |
| ability of oral bacteria to induce tissue-destructive molecules from human neutrophils. | the induction of tissue-destructive molecules from neutrophils by periodontopathic bacteria has been suggested as one of the mechanisms of periodontal destruction. the aim of this study was to determine whether the ability to stimulate neutrophils is an authentic characteristic of periodontopathic bacteria. | 2008 | 18449962 |
| reca mediated spontaneous deletions of the icaadbc operon of clinical staphylococcus epidermidis isolates: a new mechanism of phenotypic variations. | phenotypic variation of staphylococcus epidermidis involving the slime related ica operon results in heterogeneity in surface characteristics of individual bacteria in axenic cultures. five clinical s. epidermidis isolates demonstrated phenotypic variation, i.e. both black and red colonies on congo red agar. black colonies displayed bi-modal electrophoretic mobility distributions at ph 2, but such phenotypic variation was absent in red colonies of the same strain as well as in control strains wi ... | 2008 | 18454346 |
| analysis of a growth-phase-regulated two-component regulatory system in the periodontal pathogen treponema denticola. | nothing is currently known regarding the global regulatory networks of treponema denticola and other oral spirochetes. in this report, we assess the properties and potential phosphotransfer capability of a putative two-component regulatory system (tcs) of t. denticola that is formed by the products of open reading frames tde0032 (a sensor kinase) and tde0033 (a response regulator), henceforth designated atcs and atcr, respectively. using pcr and dna sequence analyses, atcs and atcr were demonstr ... | 2008 | 18621891 |
| analysis of proteinase-activated receptor 2 and tlr4 signal transduction: a novel paradigm for receptor cooperativity. | proteinase-activated receptor 2 (par2), a seven-transmembrane g protein-coupled receptor, is activated at inflammatory sites by proteolytic cleavage of its extracellular n terminus by trypsin-like enzymes, exposing a tethered, receptor-activating ligand. synthetic agonist peptides (ap) that share the tethered ligand sequence also activate par2, often measured by ca2+ release. par2 contributes to inflammation through activation of nf-kappab-regulated genes; however, the mechanism by which this oc ... | 2008 | 18622013 |
| microorganisms in root canal-treated teeth from a german population. | posttreatment apical periodontitis is usually associated with persistent or secondary intraradicular infection. this study evaluated the presence and relative levels of 28 bacterial taxa in treated root canals of teeth with posttreatment apical periodontitis from german patients using 16s ribosomal rna (rrna) gene probes in a reverse-capture checkerboard hybridization assay. species-specific polymerase chain reaction (pcr) was also performed to detect enterococcus faecalis and candida albicans. ... | 2008 | 18634922 |
| cdp-alcohol hydrolase, a very efficient activity of the 5'-nucleotidase/udp-sugar hydrolase encoded by the usha gene of yersinia intermedia and escherichia coli. | nucleoside 5'-diphosphate-x hydrolases are interesting enzymes to study due to their varied activities and structure-function relationships and the roles they play in the disposal, assimilation, and modulation of the effects of their substrates. few of these enzymes with a preference for cdp-alcohols are known. in yersinia intermedia suspensions prepared from cultures on columbia agar with 5% sheep blood, we found a cdp-alcohol hydrolase liberated to triton x-100-containing medium. growth at 25 ... | 2008 | 18641143 |
| treatment outcomes of dental flossing in twins: molecular analysis of the interproximal microflora. | the purpose of this study was to assess the effects of dental flossing on the microbial composition of interproximal plaque samples in matched twins. | 2008 | 18672992 |
| development of a transposon mutagenesis system in the oral spirochete treponema denticola. | here, we report successful transposon mutagenesis in the oral spirochete treponema denticola. a modified himar1 transposon, including a new antibiotic selection cassette for t. denticola, generated mutations affecting cell division, transport, and chemotaxis, among other processes. this random mutagenesis system should facilitate research on the biology and pathogenesis of this spirochete, which is associated with human periodontal diseases. | 2008 | 18723655 |
| clinical and microbiological benefits of systemic metronidazole and amoxicillin in the treatment of smokers with chronic periodontitis: a randomized placebo-controlled study. | the aim of this study was to evaluate the clinical and microbiological effects of scaling and root planing (srp) alone or combined with metronidazole (mtz) or with mtz and amoxicillin (amx) in the treatment of smokers with chronic periodontitis. | 2008 | 18727657 |
| treponema denticola tror is a manganese- and iron-dependent transcriptional repressor. | treponema denticola harbours a genetic locus with significant homology to most of the previously characterized treponema pallidum tro operon. within this locus are five genes (troabcdr) encoding for the components of an atp-binding cassette cation-transport system (troabcd) and a dtxr-like transcriptional regulator (tror). in addition, a sigma(70)-like promoter and an 18 bp region of dyad symmetry were identified upstream of the troa start codon. this putative operator sequence demonstrated simi ... | 2008 | 18761626 |
| importance of tlr2 in early innate immune response to acute pulmonary infection with porphyromonas gingivalis in mice. | the periodontal pathogen porphyromonas gingivalis is implicated in certain systemic diseases including atherosclerosis and aspiration pneumonia. this organism induces innate responses predominantly through tlr2, which also mediates its ability to induce experimental periodontitis and accelerate atherosclerosis. using a validated mouse model of intratracheal challenge, we investigated the role of tlr2 in the control of p. gingivalis acute pulmonary infection. tlr2-deficient mice elicited reduced ... | 2008 | 18768871 |
| photodynamic therapy as an adjunct to non-surgical periodontal treatment: a randomized, controlled clinical trial. | recent preclinical and clinical data have suggested a potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of this study was to evaluate the clinical and microbiologic effects of the adjunctive use of pdt to non-surgical periodontal treatment. | 2008 | 18771363 |
| identification of a methioninase inhibitor, myrsinoic acid b, from myrsine seguinii lév., and its inhibitory activities. | a methioninase inhibitor from myrsine seguinii was purified and identified as myrsinoic acid b. its inhibitory activities as to crude methioninase from periodontal bacteria such as fusobacterium nucleatum, porphyromonas gingivalis, and treponema denticola were determined. the ic50 values were 10.5, 82.4, and 30.3 microm respectively. | 2008 | 18776663 |
| the genome of borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne borrelia duttonii. | in an effort to understand how a tick-borne pathogen adapts to the body louse, we sequenced and compared the genomes of the recurrent fever agents borrelia recurrentis and b. duttonii. the 1,242,163-1,574,910-bp fragmented genomes of b. recurrentis and b. duttonii contain a unique 23-kb linear plasmid. this linear plasmid exhibits a large polyt track within the promoter region of an intact variable large protein gene and a telomere resolvase that is unique to borrelia. the genome content is char ... | 2008 | 18787695 |
| composition of supra- and subgingival biofilm of subjects with healthy and diseased implants. | the purpose of this study was to compare the microbial composition of supra- and subgingival biofilm in subjects with and without peri-implantitis. | 2008 | 18828812 |
| the chronicles of porphyromonas gingivalis: the microbium, the human oral epithelium and their interplay. | the microbiota of the human oral mucosa consists of a myriad of bacterial species that normally exist in commensal harmony with the host. porphyromonas gingivalis, an aetiological agent in severe forms of periodontitis (a chronic inflammatory disease), is a prominent component of the oral microbiome and a successful colonizer of the oral epithelium. this gram-negative anaerobe can also exist within the host epithelium without the existence of overt disease. gingival epithelial cells, the outer l ... | 2008 | 18832296 |
| binding of complement inhibitor c4b-binding protein contributes to serum resistance of porphyromonas gingivalis. | the periodontal pathogen porphyromonas gingivalis is highly resistant to the bactericidal activity of human complement, which is present in the gingival crevicular fluid at 70% of serum concentration. all thirteen clinical and laboratory p. gingivalis strains tested were able to capture the human complement inhibitor c4b-binding protein (c4bp), which may contribute to their serum resistance. accordingly, in serum deficient of c4bp, it was found that significantly more terminal complement compone ... | 2008 | 18832711 |
| periodontal bacterial profiles in pregnant women: response to treatment and associations with birth outcomes in the obstetrics and periodontal therapy (opt) study. | a recent clinical trial (obstetrics and periodontal therapy [opt] study) demonstrated that periodontal therapy during pregnancy improved periodontal outcomes but failed to impact preterm birth. the present study evaluated seven target bacteria, aggregatibacter actinomycetemcomitans (previously actinobacillus actinomycetemcomitans), porphyromonas gingivalis, treponema denticola, tannerella forsythia (previously t. forsythensis), prevotella intermedia, campylobacter rectus, and fusobacterium nucle ... | 2008 | 18834241 |
| tree of life based on genome context networks. | efforts in phylogenomics have greatly improved our understanding of the backbone tree of life. however, due to the systematic error in sequence data, a sequence-based phylogenomic approach leads to well-resolved but statistically significant incongruence. thus, independent test of current phylogenetic knowledge is required. here, we have devised a distance-based strategy to reconstruct a highly resolved backbone tree of life, on the basis of the genome context networks of 195 fully sequenced rep ... | 2008 | 18852873 |
| treponema denticola does not induce production of common innate immune mediators from primary gingival epithelial cells. | it has been hypothesized that the neutrophil chemoattractant interleukin-8 (il-8) forms a gradient in the oral cavity, with the highest concentration of il-8 produced closest to the bacterial biofilm. in periodontitis, this gradient is disrupted, impairing neutrophil chemotaxis to diseased sites. treponema denticola is prominently associated with periodontal disease, yet little is known about its ability to modulate the production of inflammatory mediators by epithelial cells. others have shown ... | 2008 | 18954353 |
| free soluble receptor activator of nuclear factor-kappab ligand in gingival crevicular fluid correlates with distinct pathogens in periodontitis patients. | the aim of the experiment was to investigate the levels of free soluble receptor activator of nuclear factor-kb ligand (srankl) in periodontal health and disease and their correlations to clinical parameters and important periodontal pathogens. | 2008 | 18988315 |
| the diverse chec-type phosphatases: chemotaxis and beyond. | a new class of protein phosphatases has emerged in the study of bacterial/archaeal chemotaxis, the chec-type phosphatases. these proteins are distinct and unrelated to the well-known chey-p phosphatase chez, though they have convergently evolved to dephosphorylate the same target. the family contains a common consensus sequence d/s-x(3)-e-x(2)-n-x(22)-p that defines the phosphatase active site, of which there are often two per protein. three distinct subgroups make up the family: chec, fliy and ... | 2008 | 18990184 |
| detection of helicobacter pylori dna in the saliva of patients complaining of halitosis. | helicobacter pylori infection, which causes peptic ulcers and gastric cancer, is considered a possible cause of halitosis. recently, the oral cavity was identified as a possible h. pylori reservoir, particularly in the presence of periodontal disease, which is a cause of halitosis. the purpose of this study was to evaluate by pcr the prevalence of oral h. pylori in the saliva of subjects complaining of halitosis. samples were obtained from 326 non-dyspeptic subjects, comprising 251 subjects with ... | 2008 | 19018029 |
| a bifunctional kinase-phosphatase in bacterial chemotaxis. | phosphorylation-based signaling pathways employ dephosphorylation mechanisms for signal termination. histidine to aspartate phosphosignaling in the two-component system that controls bacterial chemotaxis has been studied extensively. rhodobacter sphaeroides has a complex chemosensory pathway with multiple homologues of the escherichia coli chemosensory proteins, although it lacks homologues of known signal-terminating chey-p phosphatases, such as chez, chec, fliy or chex. here, we demonstrate th ... | 2008 | 19020080 |
| [relationship of putative periodontopathogenic bacteria and drug-induced gingival overgrowth]. | to investigate the effect of putative periodontopathogenic bacteria on the development of drug-induced gingival overgrowth (go) in renal transplant recipients. | 2008 | 19031785 |
| persistence of extracrevicular bacterial reservoirs after treatment of aggressive periodontitis. | the purpose of this study was to test the hypothesis that periodontal pathogens associated with aggressive periodontitis persist in extracrevicular locations following scaling and root planing, systemic antibiotics, and antimicrobial rinses. | 2008 | 19053921 |
| coaggregation of treponema denticola with porphyromonas gingivalis and fusobacterium nucleatum is mediated by the major outer sheath protein of treponema denticola. | coagreggation of treponema denticola with either porphyromonas gingivalis or fusobacterium nucleatum was characterized and the role of the major outer sheath protein (msp) in the coaggregation process of these bacteria was evaluated. the msp of t. denticola was found to be able to bind to p. gingivalis and f. nucleatum cells and this binding could be inhibited by msp in a concentration-dependent manner. while sodium dodecyl sulfate polyacrylamide gel electrophoresis and periodic acid-schiff (pas ... | 2008 | 19054094 |
| native cellular architecture of treponema denticola revealed by cryo-electron tomography. | using cryo-electron tomography, we are developing a refined description of native cellular structures in the pathogenic spirochete treponema denticola. tightly organized bundles of periplasmic flagella were readily observed in intact plunge-frozen cells. the periplasmic space was measured in both wild-type and aflagellate strains, and found to widen by less than the diameter of flagella when the latter are present. this suggests that a structural change occurs in the peptidoglycan layer to accom ... | 2008 | 18468917 |
| a genome-wide proteome array reveals a limited set of immunogens in natural infections of humans and white-footed mice with borrelia burgdorferi. | humans and other animals with lyme borreliosis produce antibodies to a number of components of the agent borrelia burgdorferi, but a full accounting of the immunogens during natural infections has not been achieved. employing a protein array produced in vitro from 1,292 dna fragments representing approximately 80% of the genome, we compared the antibody reactivities of sera from patients with early or later lyme borreliosis to the antibody reactivities of sera from controls. overall, approximate ... | 2008 | 18474646 |
| interaction of porphyromonas gingivalis with oral streptococci requires a motif that resembles the eukaryotic nuclear receptor box protein-protein interaction domain. | porphyromonas gingivalis initially colonizes the oral cavity by interacting with organisms in supragingival plaque, such as the oralis group of oral streptococci. this interaction involves the association of the streptococcal antigen i/ii with the minor fimbrial antigen (mfa1) of p. gingivalis. our previous studies showed that a peptide (bar) derived from antigen i/ii inhibits p. gingivalis adherence and subsequent biofilm formation on streptococcal substrates. in addition, screening a combinato ... | 2008 | 18474648 |
| [identification of periodontopathogen microorganisms by pcr technique]. | periodontitis is an inflammatory disease of the supporting tissues of teeth and is a major cause of tooth loss in adults. the onset and progression of periodontal disease is attributed to the presence of elevated levels of a consortium of pathogenic bacteria. gram negative bacteria, mainly strict anaerobes, play the major role. | 2008 | 19069337 |
| optimizing qpcr for the quantification of periodontal pathogens in a complex plaque biofilm. | quantitative pcr (qpcr) has recently been used to quantify microorganisms in complex communities, including dental plaque biofilms. however, there is variability in the qpcr protocols being used. this study was designed to evaluate the validity of two of these variables with the intent of developing a more standardized qpcr protocol. the two variables evaluated were (1) the use of dna content versus actual cell counts to estimate bacterial numbers in mixed plaque samples and (2) the effectivenes ... | 2008 | 19088882 |
| proteolytic activities of oral bacteria on prommp-9 and the effect of synthetic proteinase inhibitors. | tissue reactions to bacteria lead to proinflammatory reactions involving matrix metalloproteinases (mmps). synthetic protease inhibitors may offer new possibilities to regulate bacterial proteases. we investigated proteolytic activities of certain periodontal bacteria, their effects on the latent prommp-9, and the effects of synthetic mmp inhibitors and a serine protease inhibitor pefabloc. the strains studied were porphyromonas gingivalis, prevotella intermedia, peptostreptoccus micros, prevote ... | 2008 | 19088890 |
| tobacco use increases susceptibility to bacterial infection. | abstract: active smokers and those exposed to secondhand smoke are at increased risk of bacterial infection. tobacco smoke exposure increases susceptibility to respiratory tract infections, including tuberculosis, pneumonia and legionnaires disease; bacterial vaginosis and sexually transmitted diseases, such as chlamydia and gonorrhoea; helicobacter pylori infection; periodontitis; meningitis; otitis media; and post-surgical and nosocomial infections. tobacco smoke compromises the anti-bacterial ... | 2008 | 19094204 |