Publications

human glucokinase regulatory protein (gckr): cdna and genomic cloning, complete primary structure, and chromosomal localization.	null mutations in the glucokinase (gck) gene can cause autosomal dominant type 2 diabetes (maturity onset diabetes of the young, mody); however, mody is genetically heterogeneous. in both liver and pancreatic islet, glucokinase is subject to inhibition by a regulatory protein (gckr). given the role of gck in mody, gckr is itself a candidate type 2 diabetes susceptibility gene. here we describe the structure of full-length (2.2 kb) cdna for human gckr, from the hepatoblastoma cell line hepg2. the ...	1995	8589523
prohibitin: potential role in senescence, development, and tumor suppression.	prohibitin is an evolutionarily conserved gene with homologues found in organisms ranging from yeast to man. in man the gene is located on chromosome 17 at q21. the deduced amino acid sequences of the protein products from mouse and rat are identical; and these differ from the human protein sequence by a single conserved amino acid. prohibitin has antiproliferative activity and available data suggest a role in such diverse processes as normal cell cycle regulation, replicative senescence, cellul ...	1995	8591812
studies of the release and turnover of a human neutrophil lipocalin.	a 24-kda protein was purified from human neutrophil extracts and shown to be the newly discovered neutrophil gelatinase-associated lipocalin (ngal), based on structural and immunochemical data. a specific enzyme-linked immunosorbent assay (elisa) was developed for the determination of ngal in human plasma and tissue fluids. normal human plasma contains 72 micrograms l-1 of ngal (range 40-109 micrograms l-1) in two main forms, monomer and dimer. 35s-methionine metabolic studies of human neutrophi ...	1995	8633182
[antifebrile and anti-inflammatory effects of radix cynanchi atrati].	the water extract of radix cynanchi atrati used as intraperitoneal injection has been proved to have an obvious antifebrile++ effect on rat fever caused by 15% yeast suspension hypodermic injection as well as a significant anti-inflammatory effect. but the antiferbrile effect of its ethanol extract is not clear.	1995	8703343
validation of the (omega-1)-hydroxylation of lauric acid as an in vitro substrate probe for human liver cyp2e1.	the (omega-1)-hydroxylation of lauric acid (11-oh-la), a model substrate of fatty acids, was previously shown to be due to cyp2e1 in rat liver microsomes. the present study examined changes in hepatic cyp2e1 content and 11-oh-la in a panel of 29 human liver microsomes. the 11-oh-la activity was strongly correlated with the cyp2e1 content, quantitated by immunoblot (r = 0.75) and with four monooxygenase activities known to be mediated by cyp2e1: chlorzoxazone-6-hydroxylation (r = 0.73), 4-nitroph ...	1995	8615855
soluble factors required for nuclear protein import.		1995	8824445
molecular cloning, characterization, and mapping of a full-length cdna encoding human udp-galactose 4'-epimerase.	galactose metabolism in all organisms is catalyzed by three enzymatic steps: the galactokinase, galactose-1-phosphate uridyltransferase, and udp galactose 4'-epimerase reactions. we report here the molecular cloning, characterization, and mapping of a full-length cdna encoding human udp-galactose 4'-epimerase (gale). our cdna is 1488 bp long and matches the mrna size of 1.5 kg detected in fibroblasts and lymphoblasts. the human gale cdna encodes a predicted protein of 348 amino acids with a mole ...	1995	8593531
human reg family genes are tandemly ordered in a 95-kilobase region of chromosome 2p12.	reg, first isolated from a rat regenerating islet cdna library, is expressed in regenerating islet beta-cells. recently, it has been revealed that reg and reg-related genes constitute a multigene family, the reg family. in human, the four reg family genes, i.e., reg 1 alpha, reg 1 beta, reg-related sequence (rs) and hip/pap, have so far been isolated. in this study, we analyzed yac clones containing the four genes and performed two-color fish to determine the map order of the genes. the human re ...	1995	8549770
the yeast tribrid system--genetic detection of trans-phosphorylated itam-sh2-interactions.	protein-protein interactions are often dependent on the post-translational modification of one component of a complex. to facilitate the study of these interactions in signal transduction, we have developed the yeast tribrid system, a modification of the yeast two-hybrid system. we demonstrate that the interactions are dependent upon the presence of a tyrosine kinase, an sh2 domain and a tyrosine containing substrate. using the gamma subunit of the high-affinity ige receptor, fc epsilon ri, this ...	1995	9636306
response of the germfree rat colonic mucosa to dietary endotoxins.	suggestive to induce an immunoreactive response in the colon mucosa of germfree rats 3 diets, autoclaved or gamma-irradiated, respectively, were administered to groups of 30 days old gf ztm:sprd rats. diets and rats were studied in parallel after a feeding period of 20 days. the microbiological controls confirmed the sterility of diets and animals. the aqueous suspensions of one diet displayed obviously dead yeast and gram positive and negative bacteria, which consistently were evident in coloni ...	1996	9226963
a presynaptic inositol-5-phosphatase.	synaptojanin is a nerve terminal protein of relative molecular mass 145,000 which appears to participate with dynamin in synaptic vesicle recycling. the central region of synaptojanin defines it as a member of the inositol-5-phosphatase family, which includes the product of the gene that is defective in the oculocerebrorenal syndrome of lowe. synaptojanin has 5-phosphatase activity and its amino-terminal domain is homologous with the yeast protein sac1 (rsd1), which is genetically implicated in ...	1996	8552192
[the antimutagenic action of apiculture products].	using the battery of test-systems, we studied the possible antimutagenic activity of some products of apiculture. it is established that apilak-preparation made on the basis of queen bee milk didn't show gene-protective abilities in ames test. the rest products of apiculture: propolis, beebread, honey preparations n1 and n2, and queen bee milk manifested to some degree their ability to decrease the mutagenic effects of some chemical and physical mutagens. the toxic effect of propolis upon yeast ...	1996	9139438
isolation and characterization of glycogen synthase in dictyostelium discoideum.	we have partially purified the protein and isolated the glcs gene for glycogen synthase in dictyostelium. glcs mrna is present throughout development and is the product of a single gene coding for 775 amino acids, with a predicted molecular mass of 87 kd. the sequence is highly similar to glycogen synthase from human muscle, yeast, and rat liver, diverging significantly only at the amino and carboxy termini. phosphorylation and udpg binding sites are conserved, with k(m) values for udpg being co ...	1996	9023987
construction of the complete rat fatty acid synthase cdna and its expression in saccharomyces cerevisiae.	the 272 647-dalton polypeptide of fatty acid synthase (fas) from rattus norvegicus has been expressed in a proteinase-deficient strain of saccharomyces cerevisiae. the seven overlapping cdna clones for rat fas spanning the entire coding region were the starting material for this undertaking. in a series of cloning steps an expression plasmid was constructed in which the cdna was placed under the control of the yeast adh1 promoter. northern blotting of total rna isolated from yeast transformed wi ...	1996	8595667
human natural yeast killer toxin-like candidacidal antibodies.	a murine mab (mabkt4, igg1) that neutralized in vitro the anti-candida activity of a killer toxic (kt) from the yeast pichia anomala acted as an idiotypic (id) vaccine in eliciting anti-id abs with toxin-like activity (kt-idab) in a rat vaginitis model. in this study, we demonstrate that intravaginal or intragastric inoculations of candida albicans bearing a receptor for the toxin was able to recall kt-idab production in the vagina of the animals primarily immunized with mabkt4 and also to elici ...	1996	8596040
molecular cloning of a schizosaccharomyces pombe cdna encoding lanosterol synthase and investigation of conserved tryptophan residues.	a schizosaccharomyces pombe cdna encoding lanosterol synthase was cloned by complementing a saccharomyces cerevisiae lanosterol synthase mutant. the predicted 83-kda protein is 54-58% identical to other lanosterol synthases. the previously known lanosterol synthases contain 229 conserved residues, which should encompass the catalytically essential amino acids. this number is decreased dramatically by including the sc. pombe lanosterol synthase in the analysis; 42 residues are no longer conserved ...	1996	8604986
cloning of arabidopsis thaliana glutathione synthetase (gsh2) by functional complementation of a yeast gsh2 mutant.	glutathione (l-gamma-glutamyl-l-cysteinylglycine, gsh) plays an important role in the protection of plants against various types of stress caused by reactive oxygen species, gazeous pollutants, heavy metals and xenobiotics. a cdna fragment containing the entire coding unit for glutathione synthetase (gsh2) of arabidopsis thaliana was cloned by complementation of the methylglyoxal sensitivity of a gsh2 mutant of the yeast saccharomyces cerevisiae. the cdna encodes a protein of 478 amino acids (de ...	1996	8612643
genetic and molecular basis for copper toxicity.	recent studies resulted in the cloning of the genes responsible for menkes syndrome and wilson disease. despite the distinct clinical phenotypes of these disorders, each gene encodes a highly homologous member of the cation-transport p-type atpase family. the remarkable evolutionary conservation of these proteins in bacteria, yeast, plants, and mammals reveals a fundamental protein structure essential for copper export in all life forms. characterization of a molecular defect in the rat homologu ...	1996	8615371
functional expression of uridine 5'-diphospho-glucose 4-epimerase (ec 5.1.3.2) from arabidopsis thaliana in saccharomyces cerevisiae and escherichia coli.	it is our goal to investigate the biosynthesis of galactose-containing compounds in higher plants. searching a database of expressed sequence tags, a cdna from arabidopsis thaliana (clone 108g20t7) with sequence similarity to udp-glucose epimerase was identified and further analyzed. the 1356-bp-long cdna included an open reading frame predicted to encode a 351 amino acid protein of 39 kda. the presumed protein sequence showed a high degree of similarity to udp-glucose epimerase sequences from b ...	1996	8615692
anti-recombinogenic and convertible co-mutagenic effects of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) and other 5-substituted pyrimidine nucleoside analogs in s. cerevisiae mp1.	in experiments using yeast, without addition of an external metabolic activation system, (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) was co-mutagenic and showed an insignificant anti-recombinogenic effect in combination with triethylene melamine (tem). in the presence of activating s9-mix, the anti-recombinogenicity and co-mutagenicity could clearly be seen. at higher concentrations the co-mutagenic effect was converted into anti-mutagenicity. the other three 5-substituted pyrimidine nucleoside ...	1996	9003540
anticryptococcal activity by alveolar macrophages from rats treated with cortisone acetate during different periods of time.	the effect of cortisone acetate (ca) treatment on the anticryptococcal activity by rat alveolar macrophages (am) was investigated. the animals received a weekly dose of 5 mg ca during 1, 2, 3 or 4 weeks. following the final dose the am were collected by lung lavage and challenged with cryptococcus neoformans. parallel experiments with silica particles of a similar size were performed. the phagocytic function was assessed using a fluorescence method that distinguishes between attached and ingeste ...	1996	9144951
evaluation of the antiinflammatory activity of sodium valproate in rats and mice.	1. treatment of rats with sodium valproate (100, 200 and 400 mg/kg i.p.) reduced the paw oedema induced by carrageenan by 36, 15 and 48%, respectively, within 3 h . 2. the effect produced by the higher dose (400 mg/kg) was equivalent to that produced by indomethacin (100 mg/kg). at 100 and 400 mg/kg, sodium valproate decreased the granuloma formation by a significant level. similar doses of sodium valproate did not affect the rectal temperature in yeast-fevered mice, except with a dose of 200 mg ...	1996	9304414
the dose-related effects of paracetamol on hyperalgesia and nociception in the rat.	1. we have studied the effects of 3 low doses of paracetamol (25, 50 and 100 mg kg-1 p.o.) on inflammatory hyperalgesia, inflammatory oedema, and nociceptive thresholds in rats. 2. at the lower dose (25 mg kg-1), paracetamol reduces only central hyperalgesia. 3. at the doses of 50 and 100 mg kg-1, paracetamol reduces also peripheral hyperalgesia; moreover, it enhances nociceptive thresholds to a mechanical stimulus in the non-inflamed paws. 4. neither paw inflammatory oedema nor tail nociceptive ...	1996	8825353
pneumocystis carinii telomere repeats are composed of ttaggg and the subtelomeric sequence contains a gene encoding the major surface glycoprotein.	we have cloned a telomere and adjacent sequences from rat-derived pneumocystis carinii using the ability of foreign telomeres to complement a yeast artificial chromosome (yac) deficient by one telomere in saccharomyces cerevisiae. characterization of the cloned dna in the recombinant yac demonstrated that it was a chimera of two p. carinii sequences, namely a 13.5 kb fragment of mitochondrial dna and an 8.3 kb distal portion consisting of subtelomeric dna. the p. carinii telomere repeat was demo ...	1996	8825773
investigation of a choline phosphate synthesis pathway in streptococcus pneumoniae: evidence for choline phosphate cytidylyltransferase activity.	in this study we have demonstrated the activity of a choline phosphate cytidylyltransferase in cell free extracts of streptococcus pneumoniae. southern blot analysis of restricted s. pneumoniae genomic dna probed with the gene coding for the choline phosphate cytidylyltransferase of saccharomyces cerevisiae demonstrated that there is homology between the s. cerevisiae cct gene and genomic dna of s. pneumoniae. we believe that this enzyme is involved in the biosynthesis of the choline containing ...	1996	8837483
ott, a mouse x-linked multigene family expressed specifically during meiosis.	the tissue expression patterns of 10 mouse testis cdnas were analysed by rt-pcr to search for new mammalian meiotic genes. the homologue of the rat synaptonemal complex protein gene scp1 is expressed in embryonic ovary, adult brain and testis. one novel gene is stringently testis specific and another is expressed exclusively in testis and embryonic ovary. the latter clone is not expressed in the testes of adult sex-reversed mice which lack germ cells, and therefore represents a meiosis-specific ...	1996	8842733
pcr in diagnosis of pneumocystosis of rats.	polymerase chain reaction (pcr) is a powerful technique to detect scanty amount of dna from living organisms. the present study intended to develop specific primers for pcr diagnosis of pneumocystosis and to evaluate diagnostic efficacy by preparation of template dnas from invasive bal fluid and also to screen serum or blood as a non-invasive specimen. albino rats of wistar or fischer strains were experimentally infected by pneumocystis carinii. extracted dnas or cell lysates of their blood, bro ...	1996	8843695
chemical synthesis and purification of proteins: a methodology.	classical stepwise solid-phase peptide synthesis (spps) has been used successfully for the synthesis of proteins up to 150 residues in length, although usually with poor yields and homogeneity. the major limitation has been the inability to separate chromatographically similar deletion and truncated impurities from the target sequence. we have developed a highly effective protocol for stepwise spps and 'one-step' purification of small proteins. to demonstrate the effectiveness of the methodology ...	1996	8844261
hepatitis b virus transactivator protein, hbx, associates with the components of tfiih and stimulates the dna helicase activity of tfiih.	human hepatitis b virus genome encodes a protein, termed hbx, that is widely recognized as a transcriptional transactivator. while hbx does not directly bind cis-acting transcriptional control elements, it has been shown to associate with cellular proteins that bind dna. because hbx transactivated a large number of viral/cellular transcriptional control elements, we looked for its targets within the components of the basal transcriptional machinery. this search led to the identification of its i ...	1996	8855220
effect of suramin on the biological activity of the two isoforms of the rat 5 alpha-reductase.		1996	8870171
molecular cloning of a cdna coding for gtp cyclohydrolase i from dictyostelium discoideum.	the gtp cyclohydrolase i (gtp-ch) gene of the cellular slime mould dictyostelium discoideum has been cloned and sequenced. the 855 bp cdna of this gene contains the open reading frame (orf) encoding 232 amino acids with a predicted molecular mass of approx. 26 kda. southern blot analysis indicated the presence of a single gene for gtp-ch in dictyostelium. pcr amplification of the orf from chromosomal dna and sequencing showed the existence of a 101 bp intron in the gtp-ch gene of dictyostelium d ...	1996	8870645
caprine homologue of rodent 5'-amp-activated protein kinase subunit and yeast snf4/cat3 is down-regulated by thyroid hormone.	we isolated a cdna, b12, that was down-regulated by thyroid hormone (th) in the goat cerebellum, using a polymerase chain reaction (pcr)-based subtractive hybridization and differential screening procedure. northern blot analysis of rna from cerebellum of t4-treated and untreated hypothyroid goats confirmed that clone b12 was th-regulated with an average reduction in expression of 21% after 4 days of t4 supplementation. other tissues from a t4-treated and an untreated hypothyroid goat also revea ...	1996	8872308
involvement of the alpha subunit of farnesyl-protein transferase in substrate recognition.	using photoaffinity labeling, we have identified a region in mammalian farnesyl-protein transferase (fptase) involved in substrate recognition. the photolabel used (compound 1) is a peptide containing the photoactive amino acid p-benzoylphenylalanine (bpa). upon exposure to uv light. compound 1 inhibits fptase activity in a time- and concentration-dependent manner. photoinhibition of fptase activity by compound 1 is prevented by adding h-ras to the reaction mixture, indicating that labeling is t ...	1996	8873619
the toxic milk mouse is a murine model of wilson disease.	wilson disease (wd) is an autosomal recessive defect of copper transport characterized by massive accumulation of copper in the liver, which can lead to liver failure. mutations in a copper transporting atpase (wnd or atp7b) have been shown to cause the disease. the toxic milk mouse mutant (tx) accumulates copper in the liver in a manner similar to that observed in patients with wd. however, some physiological differences between tx mice and human wd patients have cast doubts on whether this mut ...	1996	8894697
direct inhibition of the signaling functions of the mammalian target of rapamycin by the phosphoinositide 3-kinase inhibitors, wortmannin and ly294002.	the immunosuppressant, rapamycin, inhibits cell growth by interfering with the function of a novel kinase, termed mammalian target of rapamycin (mtor). the putative catalytic domain of mtor is similar to those of mammalian and yeast phosphatidylinositol (pi) 3-kinases. this study demonstrates that mtor is a component of a cytokine-triggered protein kinase cascade leading to the phosphorylation of the eukaryotic initiation factor-4e (eif-4e) binding protein, phas-1, in activated t lymphocytes. th ...	1996	8895571
tyrosine kinase activation is an immediate and essential step in hypotonic cell swelling-induced erk activation and c-fos gene expression in cardiac myocytes.	hypotonic stress causes rapid cell swelling and initiates various cellular adaptive processes. however, it is unknown how cells initially sense low osmolarity and convert it into intracellular signals. we investigated the signal transduction mechanism initiated by hypotonic cell swelling in cardiac myocytes using c-fos expression as a nuclear marker. treatment of myocytes with hypotonic culture media rapidly induced c-fos expression, whereas hypertonic stress had no effect. transfection of c-fos ...	1996	8896447
the product of the saccharomyces cerevisiae rss1 gene, identified as a high-copy suppressor of the rat7-1 temperature-sensitive allele of the rat7/nup159 nucleoporin, is required for efficient mrna export.	rat7/nup159 was identified previously in a screen for genes whose products are important for nucleocytoplasmic export of poly(a)+ rna and encodes an essential nucleoporin. we report here the identification of rss1 (rat seven suppressor) as a high-copy extragenic suppressor of the rat7-1 temperature-sensitive allele. rss1p encodes a novel essential protein of 538 amino acids, which contains an extended predicted coiled-coil domain and is located both at nuclear pore complexes (npcs) and in the cy ...	1996	8898365
acylation of glucosaminyl phosphatidylinositol revisited. palmitoyl-coa dependent palmitoylation of the inositol residue of a synthetic dioctanoyl glucosaminyl phosphatidylinositol by hamster membranes permits efficient mannosylation of the glucosamine residue.	two critical steps in the assembly of yeast and mammalian glycosylphosphatidylinositol (gpi) anchor precursors are palmitoylation of the inositol residue and mannosylation of the glucosamine residue of the glucosaminyl phosphatidylinositol (glcnalpha-pi) intermediate. palmitoylation has been reported to be acyl-coa dependent in yeast membranes (costello, l. c., and orlean, p. (1992) j. biol. chem. 267, 8599-8603) but strictly acyl-coa independent in rodent membranes (stevens, v. l., and zhang, h ...	1996	8900192
isoform-specific purification and substrate specificity of the 5'-amp-activated protein kinase.	the 5'-amp-activated protein kinase (ampk) mediates several cellular responses to metabolic stress. rat liver contains at least two isoforms of this enzyme, either alpha1 or alpha2 catalytic subunits together with beta and gamma noncatalytic subunits in a trimeric complex. the alpha1 isoform is purified using a peptide substrate affinity chromatography column with adr1 (222-234)p229 (lkkltrrpsfsaq), corresponding to the camp-dependent protein kinase phosphorylation site in the yeast transcriptio ...	1996	8910470
endoplasmic reticulum form of calreticulin modulates glucocorticoid-sensitive gene expression.	calreticulin is a ubiquitously expressed ca2+-binding protein of the endoplasmic reticulum (er), which inhibits dna binding in vitro and transcriptional activation in vivo by steroid hormone receptors. transient transfection assays were carried out to investigate the effects of different intracellular targeting of calreticulin on transactivation mediated by glucocorticoid receptor. bsc40 cells were transfected with either calreticulin expression vector (er form of calreticulin) or calreticulin e ...	1996	8910610
rat 17 beta-hydroxysteroid dehydrogenase type iv is a novel peroxisome proliferator-inducible gene.	to better understand the molecular mechanisms of the pleiotropic responses induced by exposure to peroxisome proliferator chemicals (ppcs), we conducted a systematic search for genes whose mrna levels are modulated by the ppc wy-14,643 (wy) in rat liver. the sequence of one up-regulated cdna (2480 bp) was predicted to encode a protein of 735 aa with 82% identity to the porcine 17 beta-hydroxysteroid dehydrogenase type iv (hsd iv). like the porcine enzyme, the rat hsd iv contains' a region homolo ...	1996	8913347
isolation of a brefeldin a-inhibited guanine nucleotide-exchange protein for adp ribosylation factor (arf) 1 and arf3 that contains a sec7-like domain.	brefeldin a (bfa) inhibited the exchange of adp ribosylation factor (arf)-bound gdp for gtp by a golgi-associated guanine nucleotide-exchange protein (gep) [helms, j.b. & rothman, j.e. (1992) nature (london) 360, 352-354; donaldson, j.g., finazzi, d. & klausner, r.d. (1992) nature (london) 360, 350-352]. cytosolic arf gep was also inhibited by bfa, but after purification from bovine brain and rat spleen, it was no longer bfa-sensitive [tsai, s.-c., adamik, r., moss, j. & vaughan, m. (1996) proc. ...	1996	8917509
cloning of sponge (geodia cydonium) and tunicate (botryllus schlosseri) proteasome subunit epsilon (prce): implications about the vertebrate mhc-encoded homologue lmp7 (prcc).	proteasomes are large protein complexes that play a major role in selective degradation of intracellular proteins. eukaryotes feature seven different alpha and beta subunits. two of the vertebrate housekeeping beta-subunits have mhc-encoded homologues that can substitute for the housekeeping counterparts upon interferon-gamma induction. in the present study we report the cloning of invertebrate beta-subunit proteasome epsilon (prce), from the marine sponge geodia cydonium and from the colonial t ...	1996	8920927
copii vesicles derived from mammalian endoplasmic reticulum microsomes recruit copi.	er to golgi transport requires the function of two distinct vesicle coat complexes, termed copi (coatomer) and copii, whose assembly is regulated by the small gtpases adp-ribosylation factor 1 (arf1) and sar1, respectively. to address their individual roles in transport, we have developed a new assay using mammalian microsomes that reconstitute the formation of er-derived vesicular carriers. vesicles released from the er were found to contain the cargo molecule vesicular stomatitis virus glycopr ...	1996	8922375
cellular immune response to retinal s-antigen & interphotoreceptor retinoid binding protein fragments in idiopathic human uveitis.	the role of retinal antigens in idiopathic human uveitis has been studied in 38 patients of uveitis, and 30 patients of systemic connective tissue disease (ctd) and 30 healthy volunteers. lymphocyte proliferative responses were tested in vitro against native s-antigen, its uveitopathogenic peptides (peptide m, peptide g), yeast histone h3 peptide and uveitopathogenic fragment of interphotoreceptor retinoid binding protein (irbp: r16) to establish their role in pathogenesis of human uveitis. seve ...	1996	8935743
molecular cloning of cdna encoding rat very long-chain acyl-coa synthetase.	the cdna encoding rat very long-chain acyl-coa synthetase (vlacs) was cloned, using degenerative primers synthesized according to the partial amino acid sequences of the peptide fragments of the purified rat liver enzyme. the longest cdna insert was 2972 base pairs with a 1860-base pair open reading frame encoding 620 amino acids. the calculated molecular mass of 70,692 daltons was consistent with size of the purified enzyme. in northern blot analysis, a single band was detected at the position ...	1996	8939997
the amino acid sequence of rat kidney 5-oxo-l-prolinase determined by cdna cloning.	5-oxoprolinase (ec 3.5.2) catalyzes a reaction in which the endergonic cleavage of 5-oxo-l-proline to form l-glutamate is coupled to the exergonic hydrolysis of atp to adp and inorganic phosphate. highly purified preparations of the enzyme have been obtained from rat kidney and pseudomonas putida. the rat kidney enzyme is composed of two strongly interacting, apparently identical subunits (mr = 142,000), whereas that from p. putida is composed of two functionally different protein components tha ...	1996	8943290
the involvement of nitric oxide in the anti-candida albicans activity of rat neutrophils.	rat peritoneal neutrophils (pmn) spontaneously release nitric oxide (no) when incubated in vitro. addition of the no synthase inhibitor l-monomethylarginine (l-nmma) to the pmn reduces no production and impairs the killing of the yeast candida albicans, both effects being reversed by l-arginine. these data strongly suggest that oxidative metabolism of l-arginine by pmn is involved in the candidacidal activity of these cells. rat blood pmn, which do not produce significant amounts of no, exhibit ...	1996	8943729
carp cdna sequence encoding a putative diazepam-binding inhibitor/endozepine/acyl-coa-binding protein.	a full-length cdna coding for common carp diazepam-binding inhibitor (dbi)/endozepine (ep)/acyl-coa-binding protein (acbp) was isolated and sequenced. the deduced dbi/ep/acbp is comprised of 87 amino acids (including initiating methionine) without possessing a signal peptide. common carp dbi/ep/acbp displays 77%, 78%, 70%, 63%, 61% and 45% identity with human, bovine, rat, frog, duck and yeast dbi/ep/acbp, respectively.	1996	8948484
self-interaction of the major 27-kilodalton outer dense fiber protein is in part mediated by a leucine zipper domain in the rat.	the rt7 gene is exclusively expressed in spermatids and encodes the 27-kda major outer dense fiber (odf) protein odf27. analysis of its amino acid structure had indicated the presence of a putative leucine zipper dimerization motif in the n-terminus and the presence of pcx repeats in the c-terminus. we had previously shown that the odf27 n-terminal fragment can interact with full-length odf27. we have used two different methods to analyze this interaction further. first we used fusion proteins b ...	1996	8949892
molecular cloning and expression of a cdna encoding a protein tyrosine phosphatase from mouse brain.	a mouse brain cdna library was screened with a radioisotope-labeled probe of human placental protein tyrosine phosphatase. the isolated clone (mbptp1b) was found to contain an open reading frame of 1,296 nucleotides as well as 5' (709 nucleotides) and 3' (341 nucleotides) non-coding regions. this cdna encodes a ptp of 432 amino acids having a mass of 49,563 daltons and exhibiting 83% and 93.5% sequence identity to that of human ptp1b and rat ptp1, respectively. the expression of the cdna in yeas ...	1996	8955893
uncoupling of dna replication and cell cycle progression by human cyclin e.	the g1-specific d- and e-type cyclins are among the most crucial factors controlling cell cycle progression in mammalian cells and are therefore thought to play an important role in tumorigenisis. d-type cyclins have indeed been shown to be endowed with an oncogenic potential. here, we report that the ectopic expression of human cyclin e, but not cyclin d1, deregulates dna synthesis in both yeast and mammalian cells. in yeast, induction of dna synthesis by cyclin e occurs even under conditions o ...	1996	8957094
engineering the c-region of human insulin-like growth factor-1: implications for receptor binding.	recombinant wild-type human igf-1 and a c-region mutant in which residues 28-37 have been replaced by a 4-glycine bridge (4-gly igf-1) were secreted and purified from yeast. an igf-1 analogue in which residues 29-41 of the c-region have been deleted (mini igf-1) was created by site-directed mutagenesis and also expressed. all three proteins adopted the insulin-fold as determined by circular dichroism. the significantly raised expression levels of mini igf-1 allowed the recording of two-dimension ...	1996	8961354
mitochondrial fad-glycerophosphate dehydrogenase and g-protein-coupled inwardly rectifying k+ channel: no evidence for linkage in maturity-onset diabetes of the young or niddm.	two genes that have potentially important regulatory roles in insulin secretion are both located on chromosome 2q24.1. g-protein-coupled muscarinic potassium channel (girk1) is an inwardly rectifying k+ channel that helps to maintain the resting potential and excitability of cells. mitochondrial fad-linked glycerophosphate dehydrogenase (m-gdh) catalyzes a rate-limiting step of the glycerol phosphate shuttle in pancreatic islets. reduced m-gdh activity has been demonstrated in islets isolated fr ...	1996	8621016
mechanical stretch activates the stress-activated protein kinases in cardiac myocytes.	we have recently shown that mechanical stress activates a phosphorylation cascade of protein kinases including raf-1 and the extracellular signal-regulated kinases (erks) in cultured cardiac myocytes partially through the enhanced secretion of angiotensin ii. osmotic stress in budding yeast has been shown to activate similar signaling molecules including hog-1, a distant relative of the erk family. in the present study, we examined whether mechanical stretch of cardiac myocytes activates the str ...	1996	8621062
mammalian vesicle trafficking proteins of the endoplasmic reticulum and golgi apparatus.	vesicle traffic propagates and maintains distinct subcellular compartments and routes secretory products from their site of synthesis to their final destinations. as a basis for the specificity of vesicular transport reactions, each step in the secretory pathway appears to be handled by a distinct set of evolutionarily conserved proteins. mammalian proteins responsible for vesicle trafficking at early steps in the secretory pathway are not well understood. in this report, we describe rat sec22 ( ...	1996	8621431
a consensus camp-dependent protein kinase (pk-a) site in place of the ccn motif casein kinase ii site simian virus 40 large t-antigen confers pk-a-mediated regulation of nuclear import.	the regulation of nuclear protein transport by phosphorylation plays a central role in gene expression in eukaryotic cells. we previously showed that nuclear import of sv40 large tumor antigen (t-ag) fusion proteins is regulated by the ccn motif, comprising phosphorylation sites for casein kinase ii and the cyclin-dependent kinase cdc2, together with the nuclear localization signal. regulation of nuclear uptake by ccn motif kinase sites also holds true for the yeast transcription factor swi5 and ...	1996	8626446
requirement for the carboxyl-terminal domain of saccharomyces cerevisiae carbamoyl-phosphate synthetase.	the arginine-specific carbamoyl phosphate synthetase of saccharomyces cerevisiae is a heterodimeric enzyme, with a 45-kda cpa1 subunit binding and cleaving glutamine, and a 124-kda cpa2 subunit accepting the ammonia moiety cleaved from glutamine, binding all of the remaining substrates and carrying out all of the other catalytic events. cpa2 is composed of two apparently duplicated amino acid sequences involved in binding the two atp molecules needed for carbamoyl phosphate synthesis and a carbo ...	1996	8626695
ultrastructural morphology of intermediate forms and forms suggestive of conjugation in the life cycle of pneumocystis carinii.	the intrapulmonary life cycle of pneumocystis carinii has been proposed to include asexual reproduction of haploid trophic forms and sexual reproduction to form cysts or ascospores containing 8 intracystic bodies or spores. this study presents the ultrastructural morphology of trophic forms and developing ascospores. nuclear-associated organelles (nao) were observed in these various forms and in a binucleated trophozoite. the unique configuration of the nuclei and nao in this binucleated trophic ...	1996	8627488
genetic characterization of transactivation of the human t-cell leukemia virus type 1 promoter: binding of tax to tax-responsive element 1 is mediated by the cyclic amp-responsive members of the creb/atf family of transcription factors.	to achieve a better understanding of the mechanism of transactivation by tax of human t-cell leukemia virus type 1 tax-responsive element 1 (tre-1), we developed a genetic approach with saccharomyces cerevisiae. we constructed a yeast reporter strain containing the lacz gene under the control of the cyc1 promoter associated with three copies of tre-1. expression of either the cyclic amp response element-binding protein (creb) or creb fused to the gal4 activation domain (gad) in this strain did n ...	1996	8628284
removal of an intron with unique 3' branch site creates an amino-terminal protein sequence directing the scerv1 gene product to mitochondria.	the yeast scerv1 gene is of special interest because it has a dual function in mitochondrial biogenesis and in the regulation of the cell cycle. the recent discovery that the yeast scerv1 gene has a structural and functional human homologue initiated a detailed comparison of the genes and their structures. in addition the homologous alr (augmenter of liver regeneration) genes from rat and mouse have just been identified and it has been found that the mammalian proteins have a specific function i ...	1996	8972573
investigation of the anti-inflammatory activity of acacia nilotica and hibiscus sabdariffa.	the aqueous extracts of acacia nilotica and hibiscus sabdariffa were tested for anti-inflammatory, analgesic and antipyretic activities in animal models. acacia nilotica extract had an inhibitory effect on carrageenan induced paw edema and yeast-induced pyrexia in rats. it also produced a significant increase in the hot plate reaction time in mice. hibiscus sabdariffa extract had no effect on paw edema but had an inhibitory effect on yeast induced pyrexia and a significant effect on the hot plat ...	1996	8982438
further study on association of 5srrna-l5 protein complex and methionyl-trna to methionyl-trna synthetase in the macromolecular aminoacyl-trna synthetase complex.	to obtain direct evidence for the attachment of 5srrna-ribosomal l5 protein particles (5srnp) and methionine-trna (trna(met)) to methionyl-trna synthetase (metrs) in the macromolecular aminoacyl-trna synthetase (ars) complex of rat liver, a metrs-5srnp-trna(met) complex was dissociated from the macromolecular ars complex fraction by n-octyl-beta-d-glucoside (method i) or by omega-aminooctyl agarose (method ii) chromatography. the dissociated metrs complex fraction was purified by gel filtration ...	1996	8982850
biochemical and genetic interactions of two commercial pesticides with the monooxygenase system and chlorophyllin.	two commercial preparations of atrazine and zineb were tested on a diploid d7 strain of the yeast saccharomyces cerevisiae using cells from logarithmic growth phase (with a high level of cytochrome p-450) and from stationary growth phase. the compounds induced marked increases of both gene conversion and point mutation frequencies in the logarithmic phase cells, while in the stationary phase no genotoxic effect was observed. the results obtained employing ta98 and ta100 strains of salmonella typ ...	1996	9037261
characterization of the interaction of the monomeric gtp-binding protein rab3a with geranylgeranyl transferase ii.	the monomeric gtp-binding protein rab3a controls exocytosis in neuroendocrine and neuronal cells. like other members of the rab family, rab3a is posttranslationally modified by the addition of hydrophobic geranylgeranyl groups to its c-terminus. the geranylgeranylation reaction is catalysed by the heterotrimeric geranylgeranyl transferase ii. we describe the cdna cloning of the beta-subunit of human geranylgeranyl transferase ii by means of the yeast two-hybrid system. the human enzyme, which is ...	1996	8706741
isolation and expression of a cdna clone encoding human kynureninase.	kynureninase (l-kynurenine hydrolase), a pyridoxal-5'-phosphate-(pyridoxal-p)-dependent enzyme, catalyses the cleavage of l-kynurenine and l-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. in this report, we describe the isolation of a cdna clone encoding human kynureninase. degenerate oligonucleotides designed from the amino acid sequences of peptides from rat liver kynureninase, were used as primers for reverse-transcription pcr of rat kidney rna. the resulti ...	1996	8706755
the human mitochondrial import receptor, htom20p, prevents a cryptic matrix targeting sequence from gaining access to the protein translocation machinery.	yeast mas70p and nadh cytochrome b5 reductase are bitopic integral proteins of the mitochondrial outer membrane and are inserted into the lipid-bilayer in an nin-ccyto orientation via an nh2-terminal signal-anchor sequence. the signal anchor of both proteins is comprised of a short, positively charged domain followed by the predicted transmembrane segment. the positively charged domain is capable of functioning independently as a matrix-targeting signal in yeast mitochondria in vitro but does no ...	1996	8707817
retinoid x receptor isotype identity directs human vitamin d receptor heterodimer transactivation from the 24-hydroxylase vitamin d response elements in yeast.	unlike estrogen and progesterone receptors that operate as homodimers on response elements, retinoid x receptors (rxrs) and vitamin d receptors (vdrs) can function as heterodimers. studies concerning the significance of heterodimeric partnerships are usually performed utilizing mammalian or insect cells. these cells express endogenous nuclear receptors, making it impossible to assign a role for one receptor subtype over another while studying the function of transfected receptor(s). yeast lacks ...	1996	8721985
myosin-v: a class of unconventional molecular motors.	in this review we focus on the biochemical and structural properties of the myosin-v class of unconventional myosins as an example of the diversity of molecular motors within the myosin superfamily. a member of this class was first identified as a novel calmodulin-binding protein in mammalian brain (larson re, pitta de and ferro ja (1988). brazilian journal of medical and biological research, 21: 213-217). to date, the myosin-v class is represented by two molecules from yeast, one from nematodes ...	1996	8736123
changes in the phosphorylation of eukaryotic initiation factor 2 alpha, initiation factor 2b activity and translational rates in primary neuronal cultures under different physiological growing conditions.	phosphorylation of the alpha-subunit of eukaryotic initiation factor 2 (eif-2) is one of the best known mechanisms regulating protein synthesis in a wide range of eukaryotic cells, from yeast to human. to determine whether this mechanism operates in primary neuronal cells, we have cultured primary neuronal cells for 7 days under two optimal growing conditions, complete medium (containing 15% serum) and serum-free medium, and determined the protein synthesis rate, eukaryotic initiation 2 and 2b ( ...	1996	8737673
mitochondrial dna migration events in yeast and humans: integration by a common end-joining mechanism and alternative perspectives on nucleotide substitution patterns.	in contrast to extensive infiltration of plant nuclear genomes by mitochondrial and chloroplast dna fragments, a computer assessment method could only detect seven mitochondrial dna integration events in saccharomyces cerevisiae chromosomes and five examples of dna migration into mammalian nuclear genes. no evidence could be detected for mitochondrial dna insertion into chromosome iii of caenorhabditis elegans or in nuclear dna sequences of drosophila sp. or plasmodium falciparum. thus, the quan ...	1996	8742642
cloning of brassica napus ctp: phosphocholine cytidylyltransferase cdnas by complementation in a yeast cct mutant.	ctp:phosphocholine cytidylyltransferase is a rate-limiting enzyme in biosynthesis of phosphatidylcholine in plant cells. we have isolated four cdnas for the cytidylyltransferase from a root cdna library of brassica napus by complementation in a yeast cct mutant. the deduced amino-acid sequences of the b. napus enzymes resembled rat and yeast enzymes in the central domain. although all cytidylyltransferases ever cloned from b. napus and other organisms were predicted to be structurally hydrophili ...	1996	8756587
the pou domain of scip/tst-1/oct-6 is sufficient for activation of an acetylcholine receptor promoter.	in the pc12 neuroendocrine line, the neuronal nicotinic acetylcholine receptor alpha3 gene promoter is activated by scip/tst-1/oct-6, a pou domain transcription factor proposed to be important for regulating the development of specific neural cell populations. in this study, we have investigated the scip polypeptide domains involved in alpha3 promoter activation. the characteristics of activation by a chimeric effector in which the gal4 dna binding domain was substituted for the scip pou domain ...	1996	8756659
molecular cloning of a rna binding protein, s1-1.	s1 proteins a-d constitute a nuclear protein family that are liberated rapidly in a set from chromatin by mild digestion with a dna or rna hydrolyzing enzyme. with an anti-s1-protein b antiserum that reacted with b2, c1 and d1, a cdna clone, ps1-1, was obtained, which encoded a protein of 852 amino acids. the s1-1 protein, encoded within the cells by a mrna of 3480 nt, was a novel protein and could be distinguished from the s1 proteins b, c and d by their amino acid sequences. the s-1-1 protein ...	1996	8760884
broadly expressed snt-like proteins link fgf receptor stimulation to activators of ras.	snt was originally described as a approximately 90 kilodalton protein in neuronal precursor cells which bears affinity for the yeast cell cycle protein p13sucl and which undergoes rapid tyrosine phosphorylation following stimulation with growth factors which trigger terminal differentiation, but not by other growth factors which promote proliferation (rabin et al., 1993). we show here that similarly sized snt-like proteins (slps) are expressed in fibroblast, myoblast, and lymphoid cell lines, an ...	1996	8761293
purification and functional characterization of type ii dna topoisomerase from rat testis and comparison with topoisomerase ii from liver.	a number of studies in yeast have shown that dna topoisomerase ii is essential for chromosome condensation and disjunction during mitosis at the metaphase/anaphase transition and meiosis i. accordingly, kinetic and mechanistic studies have implied a role for topoisomerase ii in chromosome disjunction. as a step toward understanding the nature and role of topoisomerase ii in a mammalian germline in vivo, we have purified topoisomerase ii from rat testis to homogeneity and ascertained several of i ...	1996	8765751
role of translation initiation factor eif-2b in the regulation of protein synthesis in mammalian cells.		1996	8768075
high-level bacterial expression of mitochondrial transport proteins.	the inability to obtain abundant quantities of purified, functional membrane proteins has represented a significant impediment to the goal of obtaining high-resolution structural information. in this review, procedures are described which have been developed in this laboratory and enable the high-level bacterial expression and subsequent purification of functional mitochondrial citrate transport proteins from yeast and rat liver. the data that we have obtained using these procedures and related ...	1996	8786237
alveolar macrophage response to yeasts and inert particles.	interactions between alveolar macrophages (am) from rats and a yeast with relatively high pathogenicity (candida albicans), a yeast with low pathogenicity (saccharomyces cerevisiae) and an inert control particle (amorphous silica) of similar diameters, 3-4 microns, were studied. both yeasts were phagocytized significantly faster by am than were the control particles and c. albicans significantly faster than s. cerevisiae. quantitative nitroblue tetrazolium reduction by am reflecting their oxidat ...	1996	8786466
beta-catenin associates with the actin-bundling protein fascin in a noncadherin complex.	catenins were first characterized as linking the cytoplasmic domains of cadherin cell-cell adhesion molecules to the cortical actin cytoskeleton. in addition to their essential role in modulating cadherin adhesivity, catenins have more recently been indicated to participate in cell and developmental signaling pathways. beta-catenin, for example, associates directly with at least two receptor tyrosine kinases and transduces developmental signals within the wnt pathway. catenins also complex with ...	1996	8794867
phylogenetic, structural and functional characteristics of the na-k-cl cotransporter family.	bumetanide-sensitive na-k-cl cotransporters and thiazide-sensitive na-cl cotransporters comprise a family of integral membrane transport proteins, the na-k-cl cotransporter (nkcc) family. each of the members of this family is over 1,000 amino acids in length. we have multiply aligned the ten currently sequenced members of this family from human, rabbit, rodent, shark, flounder, moth, worm and yeast sources. phylogenetic analyses suggest the presence of at least six isoforms of these full length ...	1996	8801348
expression and characterization of human pancreatic preprocarboxypeptidase a1 and preprocarboxypeptidase a2.	we are investigating the potential utility of human carboxypeptidases a in antibody-directed enzyme prodrug therapy (adept). hybridization screening of a human pancreatic cdna library with cdna probes that encoded either rat carboxypeptidase a1 (rcpa1) or carboxypeptidase a2 (rcpa2) was used to clone the human prepro-cpa homologs. after expression of the respective pro-hcpa cdna in saccharomyces cerevisiae, the enzymes were purified to homogeneity by a combination of hydrophobic and ion-exchange ...	1996	8806703
expression of rat neuronal nitric oxide synthase in saccharomyces cerevisiae.	the rat neuronal nitric oxide synthase (nnos) cdna was expressed in saccharomyces cerevisiae under the control of a hybrid pgk/gal promoter, pal. galactose induction resulted in the production of a soluble 160 kda protein that was recognised by anti-neuronal nos antibody. nos activity was detected by the conversion of [3h]arginine to [3h]citrulline and required the addition of the cofactors, calmodulin and tetrahydrobiopterin. the activity was inhibited by the arginine analogues ng-nitro-l-argin ...	1996	8818271
the inhibition of antigen-induced eosinophilia and bronchoconstriction by cdp840, a novel stereo-selective inhibitor of phosphodiesterase type 4.	1. the novel tri-aryl ethane cdp840, is a potent and selective inhibitor of cyclic amp phosphodiesterase type 4 (pde 4) extracted from tissues or recombinant pde 4 isoforms expressed in yeast (ic50s: 4-45 nm). cdp840 is stereo-selective since its s enantiomer (ct 1731) is 10-50 times less active against all forms of pde 4 tested while both enantiomers are inactive (ic50s: > 100 microm) against pde types 1, 2, 3 and 5. 2. oral administration of cdp840 caused a dose-dependent reduction of interleu ...	1996	8818342
signal transduction pathway on beta-glucans-triggered hydrogen peroxide production by murine peritoneal macrophages in vitro.	it has been reported that the biological activities of several beta-glucans vary due to differences in their physicochemical properties. in this study we investigated the ability of beta-glucans to trigger h2o2 production and activate signaling pathway on peritoneal macrophages. the most effective beta-glucan tested on h2o2 production was zymocel which was a particulate preparation from the yeast cell wall. in contrast, gel-forming beta-glucans which are known as immunoenhancers did not trigger ...	1996	8820904
in vitro decrease of rat-derived pneumocystis carinii attachment induced by human natural yeast killer toxin-like antiidiotypic candidacidal antibodies.		1996	8822831
attempts to convert chymotrypsin to trypsin.	trypsin and chymotrypsin have specificity pockets of essentially the same geometry, yet trypsin is specific for basic while chymotrypsin for bulky hydrophobic residues at the p1 site of the substrate. a model by steitz, henderson and blow suggested the presence of a negative charge at site 189 as the major specificity determinant: asp189 results in tryptic, while the lack of it chymotryptic specificity. however, recent mutagenesis studies have shown that a successful conversion of the specificit ...	1996	8635580
the tc21 oncoprotein interacts with the ral guanosine nucleotide dissociation factor.	tc21 is a highly oncogenic member of the ras superfamily of small gtp binding proteins. we have used the yeast two hybrid system to identify proteins that interact with an oncogenic form of the tc21 protein. cdna clones encoding the carboxy-terminal region of the ralgds protein were isolated from human b-cell and hela cdna libraries. ralgds is an exchange factor that stimulates gdp dissociation from ral, another member of the ras superfamily of proteins. the interaction between ralgds to tc21 is ...	1996	8637701
role of the prodomain in folding and secretion of rat pancreatic carboxypeptidase a1.	pancreatic carboxypeptidase a1 (cpa1) is synthesized as an inactive precursor, procpa1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid n-terminal prodomain. purified rat procpa1 is renatured in vitro after denaturation in guanidine or in guanidine plus reducing agents. in contrast, purified cpa1 is not renatured under any of the conditions tested. while procpa1 is secreted in yeast when fused to the alpha-factor signal sequence in place of its endogenous ...	1996	8639628
a mammalian homologue of sly1, a yeast gene required for transport from endoplasmic reticulum to golgi.	we characterized rat cdnas that predict a protein, r-sly1, which is similar to sly1, a yeast protein that plays a critical role in endoplasmic reticulum to golgi apparatus vesicle trafficking. the r-sly1 gene is expressed in all tissues examined.	1996	8647468
the predominant protein-arginine methyltransferase from saccharomyces cerevisiae.	we have identified the major enzymatic activity responsible for the s-adenosyl-l-methionine-dependent methylation of arginine residues (ec 2.1.1.23) in proteins of the yeast saccharomyces cerevisiae. the rmt1 (protein-arginine methyltransferase), formerly odp1, gene product encodes a 348-residue polypeptide of 39.8 kda that catalyzes both the ng-mono- and ng, ng-asymmetric dimethylation of arginine residues in a variety of endogenous yeast polypeptides. a yeast strain in which the chromosomal rm ...	1996	8647869
human chromosome 1 localization of the gene for a prostaglandin f2alpha receptor negative regulatory protein.	a protein that copurifies with the bovine prostaglandin f2alpha (fp) receptor has been isolated and the corresponding rat cdna has been cloned. transfection experiments suggest that this protein inhibits binding of [3h]prostaglandin f2alpha ([3h]pgf2alpha) to fp. histologically, this protein (fp regulatory protein or fprp) shows a distribution coinciding well with those cells and tissues that respond to pgf2alpha. a portion of the 3' untranslated region of the human homolog to fprp was subcloned ...	1996	8655148
analgesic and antipyretic activities of an aqueous extract and of the flavone linarin of buddleia cordata.	the dried aqueous extract of leaves of buddleia cordata (loganiaceae) and its main flavonoid glycoside, linarin, have been evaluated for analgesic and antipyretic effects in mice and rats, respectively. both the extract and linarin exerted significant and dose-dependent analgesic and antipyretic activities, the first being obtained against a chemical stimulus (writhing a test in mice) and the second being obtained by a pyretogenic stimulus (yeast-induced hyperthermia test). furthermore, the resp ...	1996	8657746
changes in superoxide anion production and phagocytosis by circulating neutrophils during tumor progression in a rat model.	the functional state of circulating neutrophils was monitored in a rat model of mesoblastic nephroma during tumor progression. superoxide anion (o2.-) production in response to pma and phagocytosis of yeast particles (saccharomyces cerevisiae) were measured every second day after tumor cell implantation. both phagocytosis and pma-induced 02.- generation were found to be enhanced in the first period (on days 6, 8, and 10), while they became significantly reduced in the advanced stage of cancer (o ...	1996	8660819
molecular characterization and expression of rat acyl-coa synthetase 3.	isolation and characterization of a rat brain cdna identified a third acyl-coa synthetase (acs) designated acs3. the deduced amino acid sequence of the cdna revealed that acs3 consists of 720 amino acids and exhibits a structural architecture common to acss from various origins. acs3 expressed in cos cells was purified to near homogeneity. the purified acs3 resolved by sds-polyacrylamide gel electrophoresis into two major proteins of 79 and 80 kda. cell-free translation of a synthetic mrna encod ...	1996	8663269
characterization of multiple mrnas that encode mammalian translation initiation factor 5 (eif-5).	eukaryotic translation initiation factor 5 (eif-5) interacts with the 40 s initiation complex (40s.mrna.mettrnaf.eif-2.gtp) to promote the hydrolysis of bound gtp with the concomitant joining of the 60 s ribosomal subunit to the 40 s initiation complex to form a functional 80 s initiation complex. in this paper, the multiple mrnas that encode mammalian eif-5 have been characterized. in rat tissues, three major eif-5 mrnas of 3.5, 2.8, and 2.2 kilobases in length are detected. all major eif-5 mrn ...	1996	8663286
mammalian sly1 regulates syntaxin 5 function in endoplasmic reticulum to golgi transport.	members of the syntaxin gene family are components of protein complexes which regulate vesicle docking and/or fusion during transport of cargo through the secretory pathway of eukaryotic cells. we have previously demonstrated that syntaxin 5 is specifically required for endoplasmic reticulum to golgi transport (dascher, c., matteson, j., and balch, w. e.(1994) j. biol. chem. 269, 29363-29366). to extend these observations we have now cloned a protein from rat liver membranes which forms a native ...	1996	8663406
tmp21 and p24a, two type i proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking.	we report here on the isolation, cloning, and expression of two mr 21,000 proteins from rat pancreatic acinar cells, the rat-tmp21 (transmembrane protein, mr 21,000) and the rat-p24a. both proteins are transmembrane proteins with type i topology and share weak but significant homology to one another (23% identity). we further show the cloning and characterization of the human homologs, hum-tmp21, which is expressed in two variants (tmp21-i and tmp21-ii), and hum-p24a. tmp21 proteins and p24a hav ...	1996	8663407
association of a dynamin-like protein with the golgi apparatus in mammalian cells.	dynamins are a family of 100-kd gtpases comprised of at least three distinct gene products and multiple alternatively spliced variants. homologies with the shibire gene product in drosophila melanogaster and with vps1p and dnm1p in saccharomyces cerevisiae suggest that dynamins play an important role in vesicular transport. morphological studies have localized brain dynamin to coated pits and tubular invaginations at the plasma membrane, where it is believed to facilitate the formation of endocy ...	1996	8666662
identifying a species-specific region of yeast tf11b in vivo.	the general transcription factor iib (tfiib) is required for rna polymerase ii transcription in eukaryotes. it provides a physical link between the tata-binding protein (tbp) and the rna polymerase and is a component previously suggested to respond to transcriptional activators in vitro. in this report, we compare the yeast (saccharomyces cerevisiae) and human forms of the protein in yeast cells to study their functional differences. we demonstrate that human tfiib fails to functionally replace ...	1996	8668181