Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| intradermal testing with multiple recall antigens for identification of cell-mediated immune deficiency in homosexual men. | in a health screening project for gay men in stockholm, delayed cutaneous hypersensitivity was tested in 710 men by a commercial kit (multitest, mérieux) containing seven recall antigens and related to past hepatitis b virus (hbv) infection, various life style factors, the lymphadenopathy syndrome (las), and antibodies to human t-lymphotropic virus type iii (htlv-iii). the multiscore (ms: the sum of all positive reactions) was significantly decreased in men with the following characteristics: hb ... | 1986 | 3639801 |
| lack of genetic restriction by a potential anti-idiotype vaccine for type b viral hepatitis. | anti-idiotype (anti-id) reagents that bear an internal image capable of mimicking hepatitis b surface antigen (hbsag) were used to induce an antibody to hbsag (anti-hbs) response in both rabbits and chimpanzees. the anti-idiotype induced antibody response produced in rabbits recognized hbsag determinants associated with the induction of protective immunity against hepatitis b virus (hbv). attesting further to the specificity was the binding of the rabbit anti-idiotype to the anti-idiotype induce ... | 1986 | 3484564 |
| hepadnavirus infection of peripheral blood lymphocytes in vivo: woodchuck and chimpanzee models of viral hepatitis. | the peripheral blood lymphocytes (pbl) of five hepatitis b virus (hbv)-infected chimpanzees and 17 woodchuck hepatitis virus (whv)-infected woodchucks were examined for the presence of viral dna and rna. hbv dna was detected in the pbl of three of three chronically infected chimpanzees but in neither of two animals with acute hbv infection. whv dna was found in the pbl of 11 of 13 chronically infected woodchucks and in the pbl and bone marrow of 1 of 4 woodchucks with antibody to whv surface ant ... | 1986 | 3485201 |
| regulation of the immune response to hepatitis b virus and human serum albumin. i. hepatitis b surface antigen-induced secretion of antibodies with specificity for human serum albumin in hepatitis b-immune donors in vitro. | the circulatory pool of b cells, from donors immune to hepatitis b (hb) through natural infection, contained sensitized b cells with the capacity to secrete antibodies with specificity for human serum albumin (hsa) when stimulated with purified hepatitis b surface antigen (hbsag) in vitro. the immunoglobulin secretion was dependent upon and regulated by t cells and specifically induced, since it was not obtained in cell cultures from hb-susceptible donors. culture supernatants with anti-hsa reac ... | 1986 | 3486463 |
| preparation and characterization of human monoclonal antibodies directed against the hepatitis b virus surface antigen. | the hepatitis b surface antigen (hbsag) is highly immunogenic and induces an antibody response which is protective in vivo against hepatitis b virus (hbv) infection. human monoclonal antibodies specific for hbsag were produced, which could have potential therapeutic applications. lymphocytes obtained from a vaccinated donor were stimulated in vitro and fused with the human myeloma cell line gm 4672, and eight hybridomas were obtained. three of these clones, which reacted in an elisa against the ... | 1986 | 3489152 |
| production of a monoclonal antibody to human interferon-alpha (ifn-alpha) and its use to identify ifn-alpha-producing cells in virus infection in vivo. | a monoclonal antibody to human interferon-alpha (ifn-alpha) was produced using affinity-purified ifn-alpha, that reacted with recombinant human ifn-alpha 2, but not with ifn-alpha 1, ifn-alpha m1 or ifn-beta. indirect immunofluorescence using this monoclonal (designated 6c3) and anti-ifn-alpha polyclonal antibodies identified cells expressing ifn-alpha. after sendai virus induction of normal human buffy-coat cells the proportion of monocytes and lymphocytes expressing ifn-alpha rose progressivel ... | 1986 | 3508487 |
| nude mice bearing human primary hepatocellular carcinoma that produces hepatitis b surface, core, and e antigens, as well as deoxyribonucleic acid polymerase. | a primary hepatocellular carcinoma, from a patient carrying hepatitis b virus, was transplanted to athymic nude mice, and maintained through eight passages involving 39 mice. hepatitis b surface and e antigens were detected in the circulation of tumor-bearing mice. hepatitis b surface, core, and e antigens were demonstrated in tumor cells. hepatitis b core particles were visualized in the tumor extract by immune electron microscopy, and they exhibited an activity of deoxyribonucleic acid polymer ... | 1986 | 3509999 |
| hepatitis b virus polypeptide x: expression in escherichia coli and identification of specific antibodies in sera from hepatitis b virus-infected humans. | sequence analysis of the hepatitis b virus (hbv) genome revealed the presence of an open reading frame (orf x) which has the potential to encode a 154-amino acid polypeptide. a fusion protein containing 145 of the amino acids encoded by orf x and 8 amino acids of beta-galactosidase was expressed and characterized in bacterial extracts. immunoprecipitations with the orf x fusion protein as a radioactively labeled antigen were performed to screen sera of humans infected with hbv for the presence o ... | 1986 | 3510311 |
| taxonomic classification of human hepatitis b virus. | sufficient data have accumulated to permit the ictv study group on the nomenclature of hepatitis viruses to recognize human hepatitis b virus as a member of a unique group of viruses and to classify it, together with a number of related animal viruses, into a new family called the hepadnaviridae. over the past decade, the international committee on taxonomy of viruses (ictv) has been active in the development of a classification system for viruses. the majority of viruses infecting vertebrate ho ... | 1986 | 3516924 |
| human serum albumin and the enigma of chronic hepatitis type b. | 1986 | 3518045 | |
| clinicopathological study of fulminant hepatitis: coinfection with hepatitis b virus and delta agent. | 1986 | 3536053 | |
| a solid-phase enzyme immunoassay for the determination of igm and igg antibodies against translation products of pre-s1 and pre-s2 regions of hepatitis b virus. | the envelope of hepatitis b virus is coded for by pre-s1, pre-s2 regions and the s gene. a method was developed to determine antibody to the product of pre-s1 region (anti-pre-s1) and antibody to the product of pre-s2 region (anti-pre-s2), either of igm or igg class, by a solid-phase enzyme immunoassay. for the determination of anti-pre-s1, tubular particles containing translation products of pre-s1, pre-s2 regions and the s gene were broken into constituent envelope polypeptides and immobilized ... | 1986 | 3537138 |
| brain biopsy for encephalitis. | brain biopsy is justified in patients suspected of having encephalitis or viral encephalopathy because those patients are most likely to be helped if a diagnosis is made rapidly and with the greatest certainty possible. neurosurgeons are occasionally reluctant to undertake brain biopsy because the procedure is diagnostic rather than therapeutic in intent. however, using currently available techniques a 1 cm3 sample of brain tissue can be taken with very low risk of morbidity or mortality. we rec ... | 1986 | 3539442 |
| replication of hepatitis b virus in culture systems with adult human hepatocytes. | we developed a technique for isolation and primary culture of adult human hepatocytes from surgical liver biopsy specimens by in situ perfusion and a shaking method. cultured hepatocytes were maintained in monolayers for more than three weeks and showed morphological and functional characteristics in vivo. the cultured human hepatocytes were inoculated with hepatitis b virus (hbv). hepatitis b surface antigen (hbsag) in the medium was detected for about three weeks after inoculation, which was l ... | 1986 | 3540211 |
| a newly identified hepatitis b type virus in tree squirrels. | virus-associated particles have been isolated from the livers of three common gray tree squirrels (sciurus carolinensis pennsylvanicus) that have histological evidence of hepatitis. two of these livers were also positive by orcein staining, suggesting the presence of surface antigen in the cytoplasm of hepatocytes. fractionation of these particles by cscl density equilibrium gradient centrifugation and assay of the fractions for surface antigen, core antigen, and dna polymerase activities demons ... | 1986 | 3457384 |
| common evolutionary origin of hepatitis b virus and retroviruses. | hepatitis b virus (hbv), although classified as a double-stranded dna virus, has been shown recently to replicate by reverse transcription of an rna intermediate. also, the putative viral polymerase has been found to share amino acid homology with reverse transcriptase of retroviruses. using computer-assisted dna and protein sequence analyses, we examined the genomes of 13 hepadnavirus isolates (nine human, two duck, one woodchuck, and one ground squirrel) and found that other conserved regions ... | 1986 | 3458214 |
| extrachromosomal sequences of hepatitis b virus dna in peripheral blood mononuclear cells of acquired immune deficiency syndrome patients. | the primary etiologic agent of the acquired immune deficiency syndrome (aids) is a human t-lymphotropic retrovirus (the aids virus). however, the pathogenesis of this virus suggests that other cofactors may contribute to the development of clinically overt disease. the hepatitis b virus (hbv) has been implicated as a potential cofactor because hbv and aids virus infections frequently coexist, striking similarities exist in their epidemiologic patterns, and recent data indicate that hbv is lympho ... | 1986 | 3461456 |
| high affinity binding site for nuclear factor i next to the hepatitis b virus s gene promoter. | the hepatitis b virus (hbv) surface antigen (hbsag) is encoded by the s gene under the regulation of a promoter in the pre-s1 region. the s gene promoter does not contain a 'tata' box-like sequence, but there is a sequence resembling, in part, the late promoter of simian virus 40 (sv40). in an attempt to study the regulation of the s gene promoter we looked for cellular proteins which bind to this region. we report here that a nuclear protein is tightly bound to the hbv genome at a position appr ... | 1986 | 3463507 |
| unusually high-level expression of a foreign gene (hepatitis b virus core antigen) in saccharomyces cerevisiae. | as a model system for the study of factors affecting gene expression, hepatitis b virus core antigen (hbcag) has been expressed in the yeast saccharomyces cerevisiae. the singularly high levels of expression achieved are approx. 40% of the soluble yeast protein. the hbcag polypeptides are present as 28-nm particles which are morphologically indistinguishable from hbcag particles in human plasma and are highly immunogenic in mice. the plasmid construction employed to achieve these very high level ... | 1986 | 3542716 |
| expression of the hepatitis b virus large envelope protein in saccharomyces cerevisiae. | the yeast vector ppv2 has been constructed for inducible expression of non-fused proteins from the pho5 promoter. signals of the ura3 gene are used for transcription termination. the 226-amino-acid 'major' and the 389-amino-acid 'large' envelope protein of hepatitis b virus (hbv) have been produced in saccharomyces cerevisiae following insertion of the s gene or of the entire pre-s region and the s gene, respectively, of hbv into ppv2. although normally only a minor constituent of the viral enve ... | 1986 | 3549460 |
| antioxidant systems in tumour cells: the levels of antioxidant enzymes, ferritin, and total iron in a human hepatoma cell line. | the human hepatoma cell line hep 3b, which has the hepatitis b virus genome, shows over 80% decrease of copper/zinc superoxide dismutase activity, over 90% decrease of manganese superoxide dismutase activity, over 70% decrease of catalase activity, absence of glutathione peroxidase and glutathione s-transferase activities, over 270-fold increase of ferritin content and 25-fold increase of total iron compared to normal autopsy liver. these conditions of low antioxidant enzyme activities and iron ... | 1986 | 3505892 |
| bioassay for specific dna sequences using a non-radioactive probe. | a novel method for detecting specific dna sequences is described. the method uses a non-radioactive dna probe, called a probe-vector, that can transform competent escherichia coli cells at high efficiency only when it has hybridized to a specific dna target, thus forming a circular, double-stranded, plasmid-like molecule. the probe-vector carries a plasmid origin of replication and a gene that confers antibiotic resistance on transformed e. coli. the output of the assay--colored bacterial coloni ... | 1986 | 3569918 |
| natural duck hepatitis b virus infection in australia. | natural duck hepatitis b virus (dhbv) infection has been studied in australian ducks. sera from 430 pekin aylesbury cross-bred ducks taken from three separate flocks were examined for duck hepatitis b virus (dhbv) dna using a dna dot hybridisation assay. in one flock there was significant infection with dhbv (98/140), but none was detectable in the other two flocks. episomal dhbv dna in liver tissue was demonstrated by gel blot hybridisation only in birds with dhbv dna in serum. polymerase-label ... | 1986 | 3579742 |
| response of dairy calves to vaccinia viruses that express foreign genes. | repeated intradermal inoculations of calves with wild-type vaccinia virus and recombinant vaccinia viruses expressing human hepatitis b virus surface antigen and herpes simplex virus, type 1, glycoprotein d produced characteristic pox lesions at each site of injection. in some instances, calves were inoculated as many as five times at intervals from 4 to 7 weeks. the lesions invariably were more severe after the second inoculation. subsequent inoculations produced a less severe area of redness, ... | 1986 | 3700615 |
| hepatitis b virus associated dna polymerase inactivation in factor ix concentrates. | inactivation of hepatitis b virus associated dna polymerase was studied in factor ix concentrate (coagulation factors ii, vii, ix and x) by heat pasteurization (60 degrees c, 10 hr) and by alkylating agents iodoacetic acid and iodoacetamide. dna polymerase appeared to reach a residual level which occurred in human serum albumin at 60 degrees c, 10 hr under comparable spike level of hepatitis b virus. of the four coagulation factors, factor ix activity was most susceptible to inactivation procedu ... | 1986 | 3705011 |
| histopathological & immunohistochemical changes in placenta due to acute viral hepatitis during pregnancy. | 1986 | 3733185 | |
| immunochemical structure of the hepatitis b surface antigen vaccine--ii. analysis of antibody responses in human sera against the envelope proteins. | antibody responses to the three envelope (env) proteins of hepatitis b viral particles (hb-vp): the s-encoded p25 polypeptide; the pre-s(2)- and s-encoded gp33/gp36 polypeptide; and the large entire env gene (pre-s + s) product, p39/gp42, were investigated using a western immunoblotting assay (wiba). hb-vp proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and transferred to nitrocellulose by electroblotting were used as antigenic probes to determine the p ... | 1986 | 3748012 |
| genotoxicity of chemical and physical agents in cultured human tissues and cells. | in vitro model systems have recently been developed to investigate the toxicity of chemical, microbial and physical agents in normal human tissues and cells from many of the major tissue sites of high cancer incidence. pathobiological endpoints used in these studies include alterations in incorporation rates of precursors into dna, rna and protein, in the clonal growth rate of cultured cells and in dna structure (e.g. single-strand breaks, dna-protein crosslinks and chemical-dna adducts) and the ... | 1986 | 3781423 |
| failure to detect polyalbumin-binding sites on the woodchuck hepatitis virus surface antigen: implications for the pathogenesis of hepatitis b virus in humans. | binding sites for polymerized albumin on hepatitis b virus components were reported in human hepatitis b virus chronic carriers predominantly with active viral replication (hb e antigen positive). the presence of comparable albumin-binding sites in the woodchuck hepatitis virus (whv) model was examined on whv components obtained from woodchucks with active viral replication (dna polymerase positive). binding sites for polymerized woodchuck serum albumin were not detected on the intact whv virion ... | 1986 | 3783821 |
| [expression of hepatitis b gene s inserted into recombinant adenoviruses]. | 1986 | 3785249 | |
| cryoglobulinemia secondary to acute viral hepatitis. evaluation of a hospital study. | in the present study the possible relationships between cryoglobulins and acute hepatitis of different viral etiology have been investigated. in most cases, cryoglobulins represent a transient phenomenon limited to the acute phase of the disease. conversely, in 2 cases the persistence of cryoglobulins was associated with a histological pattern of chronic persistent hepatitis. | 1986 | 3787098 |
| type d hepatitis: the clinical significance of hepatitis d virus rna in serum as detected by a hybridization-based assay. | hepatitis d virus is a defective human pathogen that requires hepatitis b virus for its replication. a hybridization-based assay for the 1.75 kb rna genome of hepatitis d virus was developed using as probe a radiolabeled transcript of a cloned cdna fragment (pkd3 hepatitis d virus dna). sera from 120 chronic carriers of hbsag with confirmed hepatitis d virus infection were analyzed for the presence of hepatitis d virus rna. serum hepatitis d virus rna was detected in 43 of 74 (58%) patients with ... | 1986 | 3793007 |
| serum autoantibodies reacting with the hepatic asialoglycoprotein receptor protein (hepatic lectin) in acute and chronic liver disorders. | circulating autoantibodies reacting with affinity-purified, hepatic asialoglycoprotein receptor protein, hepatic lectin (hl), were detected by radioimmunoassay in 15 (83%) of 18 patients with autoimmune chronic active hepatitis (ai-cah) who had active disease, at titres that showed a positive correlation (p less than 0.05) with severity of periportal inflammation assessed histologically. in contrast, 10 ai-cah patients whose disease was in remission were all anti-hl seronegative. anti-hl was als ... | 1986 | 3794301 |
| [serologic and hepatic correlation of the markers of hepatitis b and d virus]. | 1986 | 3796120 | |
| effects of adenine arabinoside on serum and intrahepatic replicative forms of duck hepatitis b virus in chronic infection. | adenine arabinoside is an antiviral agent which has been used in a number of clinical studies for the treatment of chronic infections with hepatitis b virus. in order to better understand its effects and mode of action, we treated ducks chronically infected with duck hepatitis b virus with a 2-week course and monitored the effects of the drug on viral replication by studying duck hepatitis b virus dna in liver and serum using molecular biological techniques. we found the drug to be effective in ... | 1987 | 3804202 |
| hepadnaviruses and retroviruses share genome homology and features of replication. | the hepadnavirus family includes hepatitis b virus (hbv), woodchuck hepatitis virus (whv), ground squirrel hepatitis virus (gshv) and duck hepatitis b virus (dhbv). these viruses share unique ultrastructural, molecular and biological features. hbv has great medical importance in many parts of the world. more important numerically than acute hepatitis b in high prevalence geographic regions is the liver disease associated with chronic infection. there appear to be more than 200 million chronicall ... | 1987 | 3804216 |
| replication strategy of human hepatitis b virus. | to study the replication strategy of the human hepatitis b virus, the 5' end of the rna pregenome and the initiation sites of dna plus and minus strands have been mapped. the rna pregenome was found to be terminally redundant by 120 nucleotides; it is initiated within the pre-c region and may also function as mrna for synthesis of the major core protein and the hepatitis b virus reverse transcriptase. the hepatitis b virus dna minus strand is initiated within the direct repeat sequence dr1, it c ... | 1987 | 3806799 |
| vibrio vulnificus infection after raw oyster ingestion in a patient with liver disease and acquired immune deficiency syndrome-related complex. | sepsis, peritonitis, and gastroenteritis developed in a 45-yr-old homosexual man 1 day after ingestion of raw oysters. the patient had chronic active hepatitis and cirrhosis with hepatitis b virus and delta-infection. he also had persistent generalized lymphadenopathy associated with htlv-iii antibody positivity. vibrio vulnificus was isolated from the patient's blood and peritoneal fluid as well as from the same batch of oysters at the restaurant where the patient had visited. to our knowledge, ... | 1987 | 3817400 |
| characterisation of the polymerised and monomeric human serum albumin binding sites on hepatitis b surface antigen. | receptors for polymerised human albumin are present on the pre-s sequence of the envelope protein of hbv and on the hepatocyte membrane and are thought to be involved in uptake of the virus by hepatocytes. using a solid phase radioimmunoassay we demonstrate binding of hbsag to polymerised human serum albumin (phsa) in both hbe antigen-positive and -negative patients, and this binding is linearly related to the hbsag titre in both groups. there are probably several modes of interaction between hb ... | 1987 | 3794674 |
| human growth hormone induced modulation of hepatitis b virus (hbv) gene expression. | a well characterized human hepatocellular carcinoma cell line (plc/prf/5) containing complete sequences of hbv-dna in integrated form into host dna and growing under serum-free conditions was used to test the effect of human growth hormone (hgh) on the expression of the integrated viral dna. data in our hands point to an early, specific effect of hgh on hbv-dna integrated sequences which appears to be unrelated to the late effect on total protein synthesis. moreover this approach enabled us to d ... | 1987 | 3582766 |
| 2',5'-oligoadenylate synthetase activity in peripheral blood lymphocytes as a clinical marker in interferon therapy for chronic hepatitis b. | treatment of chronic hepatitis b (chb) with human leukocyte interferon (ifn-alpha) was studied in terms of increase of 2',5'-oligoadenylate (2-5a) synthetase activity in peripheral blood lymphocytes (pbl) after ifn-alpha administration. the 2-5a synthetase activity in pbl increased to a maximum 16-24 h after ifn-alpha injection and then gradually decreased, while serum hb virus dna polymerase (hbv-dnap) activity, which corresponds to the amount of hbv virions in the serum, decreased to a minimum ... | 1987 | 3585079 |
| a sequential study of viral dna in serum in experimental transmission of duck hepatitis b virus. | to understand the relationship among the time of infection, infection patterns, and liver diseases, experimental transmission of duck hepatitis b virus (dhbv) utilizing 165 japanese white domestic ducklings was performed. twenty to 25 ducklings were each inoculated with dhbv-positive serum intravenously at day one, 3, 5, 7, 10, and 14 posthatch and were sacrificed during the 1st, 2nd, 3rd, and 4th (and 24th in those inoculated on day one and day 3 posthatch) week after inoculation to obtain sera ... | 1987 | 3585287 |
| indian palm tree squirrel hepatitis b virus infection model for the human hepatitis. | 1987 | 3596679 | |
| effect of cianidanol on natural killer cell activity in patients with chronic b virus hepatitis. | the hepatoprotective antioxidant bioflavonoid cianidanol has beneficial therapeutic and immunomodulatory effects in chronic hepatitis. its action on natural killer (nk) cell activity has not yet been studied in hepatitis b virus (hbv) infection. in the present study, the in vitro and in vivo effects of the drug on nk cell activity have been determined in six patients with chronic hbv hepatitis and in ten healthy control subjects. two methods were used: an enzyme release assay and a cytotoxicity ... | 1987 | 3596873 |
| changes of hepatitis b virus (hbv) markers during prolonged recombinant interferon alpha-2a treatment of chronic hbv infection. | eleven hbsag chronic carriers were treated with recombinant-interferon (rifn)-alpha-2a with either 20 x 10(6) (n = 6) or 10 x 10(6) iu/m2 body surface (n = 5), i.m. twice weekly for 6 months. hbv-markers were tested monthly for 15 months. throughout the follow-up, 6 patients (54%) became hbeag, hbv-dnap and hbv-dna negative (responders). in addition, 8 were hbcag-negative, 10 anti-hbc-igm-negative and 2 hbsag/igm complexes negative. all patients gave polymerized human serum albumin receptors and ... | 1987 | 3571931 |
| a virus in the indian palm tree squirrel that resembles the human hepatitis b virus. | 1987 | 3507395 | |
| interactions between hepatitis b virus and polymeric human serum albumin. ii. development of syngeneic monoclonal anti-anti-idiotypes which mimic hepatitis b surface antigen in the induction of immune responsiveness. | we used monoclonal anti-idiotypes (anti-id) 63.14, previously shown to mimic polymeric human serum albumin (polyhsa) and bind its receptor on hepatitis b surface antigen (hbsag), to produce syngeneic monoclonal anti-anti-id (ab3) which could bear the internal image of hbsag and mimic its immunogenicity in vivo. nine hybridomas obtained from spleen cells of balb/c mice immunized with 63.14 were isolated, which were able to inhibit the binding of alkaline phosphatase-conjugated 63.14 to hbsag. bot ... | 1987 | 3569404 |
| natural history of acute hepatitis b surface antigen-positive hepatitis in greek adults. | we prospectively followed up 821 adults with acute viral hepatitis hospitalized at the athens hospital for infectious diseases between may 1981 and may 1983. radioimmunoassays for the detection of serologic markers of hepatitis a virus, hepatitis b virus, and hepatitis delta virus, and molecular hybridization techniques for the detection of serum hepatitis b virus deoxyribonucleic acid and hepatitis delta virus ribonucleic acid were used. based on the results of an enzyme immunoassay for the det ... | 1987 | 3569758 |
| pre-s encoded surface proteins in relation to the major viral surface antigen in acute hepatitis b virus infection. | the role of pre-s encoded viral surface proteins in acute hepatitis b virus infection is still poorly understood. binding sites for polymerized human serum albumin have been found to be encoded by the pre-s2 region of the hepatitis b virus genome. recently, murine monoclonal antibodies against pre-s1 and pre-s2 encoded hepatitis b virus gene products were generated and used for their specific detection in serum. in sera from patients with acute hepatitis b, pre-s1 and pre-s2 antigen occurred in ... | 1987 | 3569761 |
| hepatocarcinogenicity of the woodchuck hepatitis virus. | during investigations of the evolution of experimental laboratory infections of woodchucks (marmota monax) with the woodchuck hepatitis virus (whv), eight hepatocellular carcinomas (hcc) were observed, six in newborns and two in young adult animals, all within 17-36 months after infection. the absence of an external cocarcinogenic effect in the well-monitored woodchucks indicates the carcinogenicity of whv and suggests the same for the genetically and biologically similar human hepatitis b virus ... | 1987 | 3468514 |
| microbial oncogenesis. | for more than a century, medical investigators have sought to incriminate microorganisms in the cause of cancer. the first scientific evidence of such a relationship came in 1911, with the first successful induction of a tumor using a cell-free extract. since that time, considerable data have accrued linking retroviruses, herpes viruses, the hepatitis b virus, papovaviruses, and adenoviruses to various malignant neoplasms. there is also increasing evidence that certain bacteria and parasites par ... | 1987 | 3541601 |
| suramin treatment for chronic active hepatitis b--toxic and ineffective. | suramin has recently been shown to inhibit the activity of the duck hepatitis b virus dna polymerase (dhbv dnap) in vitro. however, we found no demonstrable in vivo suppression of human hepatitis b virus dna polymerase (hbv dnap) activity in three male patients with severe chronic active hepatitis. suramin treatment resulted in prolongation of the prothrombin time in all cases and a rise in bilirubin in two and it may have led to haemorrhage from oesophageal varices in one patient and to hepatic ... | 1987 | 3491874 |
| expression of the hepatitis b virus x gene in mammalian cells. | the hepatitis b virus (hbv) dna contains an open reading frame (orf) designated x, which has the capacity to encode a protein of 16,560 da (subtype adw). such a protein has not been identified in either hbv particles or infected human livers, and therefore its role in the viral life cycle remains unknown. we report here the expression of the hbv x orf in cultured cells using recombinant vectors. a protein of 16 kda was identified by means of an antiserum prepared against a synthetic peptide and ... | 1987 | 3494252 |
| modulation of the immunological response to hepatitis b virus by antibodies. | antibodies to hbsag of igg class enhanced the helper activity of a human t cell clone to promote the in vitro synthesis of immunoglobulins by autologous b lymphocytes. using two different assay systems, the effect of antigen-specific antibodies on the helper function of a hbsag-reactive t cell clone was studied. the monoclonal antibody to hbsag a5c3 (igg) increased significantly the t cell-dependent production of immunoglobulins by staphyloccocus aureus-stimulated autologous b lymphocytes. furth ... | 1987 | 3494654 |
| effects of human recombinant interleukin 2 in patients with chronic hepatitis b: a preliminary report. | five patients with hbe antigen-positive chronic active hepatitis were treated with recombinant interleukin 2 (r-il2) for 3 to 4 wk. r-il2 inhibited hbv replication during therapy as judged by decrease of serum dna polymerase activity. two of five patients lost hbeag from serum. furthermore, characteristic increases of serum aminotransferase were observed during r-il2 therapy. this rise might have been mediated by enhanced immune responses to hepatitis b virus and increased rate of lysis of the i ... | 1987 | 3495171 |
| recovery of immunodeficient mice from a vaccinia virus/il-2 recombinant infection. | vaccinia virus recombinants that express cloned genes encoding antigens of unrelated infectious agents, such as hepatitis b virus and human immunodeficiency virus (hiv), provide a new approach to the development of live vaccines. although there is evidence that genetically engineered vaccinia viruses have reduced pathogenicity a major obstacle to their use as vaccines is that severe complications can occur after vaccination, especially in immunodeficient individuals. we describe here a recombina ... | 1987 | 3498904 |
| characterization of cell mediated immune responses to the hepatitis b core protein in man. | eight donors immune to hepatitis b (hb) after natural infection were studied for their cell-mediated immune response to hepatitis b core (hbc) protein in vitro. significant specific activation was observed in highly purified peripheral blood lymphocytes and t cells from these donors after 5-8 days of incubation with hbc protein. this in vitro response was modulated by monocytic cells and maximal 3h-thymidine incorporation was elicited with low concentrations of the antigen (0.1-10 ng/ml). the ce ... | 1987 | 3652517 |
| heat-regulated expression of the hepatitis b virus surface antigen in the human wish cell line. | the dna fragment coding for the hepatitis b virus surface antigen (hbsag) was placed under the control of a human 70 kda heat-shock protein (hsp70) promotor sequence. this plasmid construct has been used in transfection experiments to establish a stable amnion cell line of human origin (wish), expressing an hbsag in a heat-regulated fashion. post-translational modifications, such as assembly, glycosylation, secretion and production of both major and middle s proteins appear to function normally. ... | 1987 | 3660944 |
| relation between hbsag binding with polymerized human serum albumin and hbv replication. | the binding between hepatitis b surface antigen (hbsag) and polymerized human serum albumin (poly-hsa) was studied in hbsag-negative (25) and hbsag-positive (92) sera by a sensitive enzyme-linked immunosorbent assay technique, and a correlation of binding activity was made with hbe-markers and hepatitis b virus-specific dna polymerase. the binding could be detected only in hbsag-positive sera and was found to be independent of the presence of hbe-markers and dna polymerase activity. further, bin ... | 1987 | 3667186 |
| free and integrated forms of hepatitis b virus dna in human hepatocellular carcinoma cells (plc/342) propagated in nude mice. | primary hepatocellular carcinoma cells (plc/342) propagated in nude mice produce hepatitis b surface antigen of subtype adr, as well as core particles containing viral dna and dna polymerase. free and integrated forms of hepatitis b virus (hbv) dna in the tumor were isolated by molecular cloning, and their nucleotide sequences were determined. both of the two representative clones of free hbv dna had the same genomic length (3,158 base pairs) and had two stop codons as well as two deletions in t ... | 1987 | 3669152 |
| interaction between human albumin polymers and the envelope polypeptide of hepatitis b virus (p31) containing the translation product of the pre-s2 region. | two preparations of human serum albumin (cohn fraction v), when subjected to high-performance liquid chromatography, were found to contain albumin polymers with molecular sizes of 35 x 10(4) daltons or greater (breakthrough fraction), as well as tetramers, trimers and dimers. they bound to the envelope polypeptide of hepatitis b virus composed of translation products of the pre-s2 region and the s gene (p31), carrying the receptor for polymerized human serum albumin, with an activity increasing ... | 1987 | 3669400 |
| virus safety of a pasteurized antithrombin iii concentrate. preclinical and clinical data. | blood derived products carry the risk of virus transmission, especially for the hepatitis b virus (hbv), non-a/non-b virus(es) (nanbv) and human immunodeficiency virus (hiv). the precautionary measures for guaranteeing the virus safety of the pasteurized antithrombin iii concentrate kybernin hs/p are described and the efficacy of these measures is demonstrated by in vitro studies and by chimpanzee trials. the inactivation rate is greater than or equal to 10(6.7) for hiv, greater than or equal to ... | 1987 | 3675669 |
| the duck hepatitis b virus pre-c region encodes a signal sequence which is essential for synthesis and secretion of processed core proteins but not for virus formation. | analysis of the serum of duck hepatitis b virus (dhbv)-infected ducks has revealed the presence of c-terminally truncated viral core proteins (e antigens). these proteins are glycosylated and therefore were not released from infected cells by lysis but rather by active secretion, indicating that the dhbv core protein can be synthesized alternatively as a cytoplasmic or a secretory protein. transient expression of cloned wild-type dhbv dna and of a specifically designed viral mutant in a human he ... | 1987 | 3682059 |
| prevalence of antibody to human immunodeficiency virus and hepatitis b surface antigen in blood samples submitted to a hospital laboratory. implications for handling specimens. | the prevalence of hepatitis b surface antigen (hbsag) and antibody to human immunodeficiency virus (hiv) was determined in serum or plasma specimens of 506 patients submitted to the clinical chemistry laboratory of an urban teaching hospital, and the results were correlated with "biohazard" warning labels on the specimens. hepatitis b surface antigen, hiv antibody, or either of these were present in 32 (6.3%), 15 (3.0%), and 44 specimens (8.7%), respectively. ten (67%) of 15 specimens with hiv a ... | 1987 | 3682137 |
| hepatitis b core and surface antigen expression in hbeag and hbv dna positive chronic hepatitis b: correlation with clinical and histological parameters. | the interrelationship between hepatitis b virus (hbv) infection, hepatic injury and clinical activity in chronic hbv infection is incompletely understood. we have scored histologic activity, the expression of hepatitis b core (hbcag) and hepatitis b surface antigen (hbsag) and assessed hbv replication to correlate hbv antigen expression with histologic disease. forty-seven formalin-fixed, percutaneous liver biopsies from hbeag carriers were studied. twenty-nine were black, 16 caucasian and two o ... | 1987 | 3683095 |
| the immune response against pre-s2-encoded peptides and human serum albumin during hepatitis b virus infection. | serum antibodies to human serum albumin (hsa) and its receptor structures (pre-s2) on hbv/hbsag particles of igg class were measured in patients with naturally acquired immunity to hepatitis b, hbsag carriers with chronic liver disease, 'healthy' symptomless hbsag carriers and controls. elisa systems in which serum samples were diluted in 0.05 m pbs to give low protein concentrations followed by incubation at 4 degrees c were used. anti-hsa and anti-pre-s2 were produced in patients during the ac ... | 1987 | 3684844 |
| viral hepatitis and pregnancy in kuwait. | the frequency and severity of viral hepatitis among pregnant and non-pregnant women in kuwait was studied from 1980 to 1984. 542 female hepatitis patients were investigated, of whom 52 (9.6%) were pregnant. 35 of the 52 (67.3%) cases of viral hepatitis in pregnancy were due to hepatitis b virus while 11 of 52 (21.2%) and 6 of 52 (11.5%) had acute hepatitis non-a, non-b (nanb) and hepatitis a virus infections, respectively. the frequency and severity of viral hepatitis among the pregnant women wa ... | 1987 | 3686634 |
| different degree of antibody response to hepatitis b virus vaccine in breast- and formula-fed infants born to hbsag-positive mothers. | antibody response to hepatitis b virus vaccine was compared in 47 breast- and 112 formula-fed infants born to hepatitis b surface antigen-(hbsag-) positive mothers. no difference was observed as to the percentage of infants who seroconverted. however, formula-fed infants developed transient but significantly higher anti-hbs antibody levels as compared to breast-fed infants. suppressive factors in human milk or orally induced tolerance may explain this finding. the latter hypothesis may be suppor ... | 1987 | 3694344 |
| expression and replication of the hepatitis b virus genome under foreign promoter control. | a novel expression system was established that allows expression and propagation of the human hepatitis b virus (hbv) genome in cultured animal cells. an overlength hbv genome encoding the viral pregenomic rna was put under transcriptional control of the human metallothionein iia promoter thereby replacing the endogenous hbv core gene promoter. transient expression of this construct in hepatoma cells resulted in formation of particles indistinguishable from hbv (dane particles). uncoupling of th ... | 1987 | 3697090 |
| [genetic transformation of somatic cells. xiv. expression of gene coding for the human hepatitis b virus surface antigen in mammalian cells]. | a set of recombinant plasmids with a gene encoding surface antigen of hepatitis b virus (hbsag) is constructed. the plasmids were transfected by deae-dextran method into different lines of cultured cells and transient expression of the hbsag gene was studied. the results indicate that: transcriptional enhancer of hepatitis b virus situated downstream from hbsag gene is active in green monkey kidney cells (cvi), promoter of 5 ltr of bovine leukemia virus is trans-activated in the goat or calf cel ... | 1987 | 3603707 |
| structure of the oligosaccharide portion of human hepatitis b surface antigen. | the hepatitis b surface antigen, which constitutes the currently available vaccine, is the empty envelope of the hepatitis b virus. we investigated the carbohydrate structures of the envelope glycoproteins. the intact oligosaccharides were enzymatically released from the coat glycoproteins using peptide-n4-(n-acetyl-beta-glucosaminyl) asparagine amidase f and isolated by gel permeation chromatography. cesium ion liquid secondary ion mass spectra of the intact, underivatized oligosaccharides show ... | 1987 | 3606122 |
| the effect of recombinant alpha-interferon treatment on serum levels of hepatitis b virus-encoded proteins in man. | the effect of alpha-interferon treatment on serum levels of hepatitis b virus-encoded proteins was analyzed in eight patients with chronic type b hepatitis who participated in a pilot study of interferon therapy. three individuals became hbsag-negative, 4 lost hbeag but remained hbsag-positive and 1 remained positive for both hbsag and hbeag. initiation of interferon treatment was rapidly followed by reduction or loss of hepatitis b virus dna in the serum but by little immediate change in hepati ... | 1987 | 3610048 |
| hepatitis b virus in tissue culture systems. i. serial propagation of virus in human macrophages. | human peritoneal macrophages, originating from peritoneal dialysis fluid and growing in vitro, support the growth and serial propagation of hepatitis b virus (hbv). the evidence for virus growth is based on the regular detection of hb core antigen in twenty-one passages of macrophages by a complement fixation test using rabbit anti-hb core serum; detection of hb core and e antigen in the twenty-first passage, using an enzyme-linked immunosorbent assay; the detection of hb antigen in cultured mac ... | 1987 | 3610569 |
| intra blood-cerebrospinal fluid-barrier detection of hepatitis b virus. | hepatitis b, a major public health concern worldwide, is caused by hepatitis type b virus, a hepdnavirus that infects only human and certain nonhuman primates, and replicates strictly in hepatocytes. by using the techniques of slot and southern blot dna hybridization, and electron microscopy, the presence of hbv was identified in the cerebrospinal fluid of three affected individuals. | 1987 | 3619890 |
| hepatitis b virus in human follicular fluid. | 1987 | 3622802 | |
| liver diseases in the indian pekin duck associated with a human hepatitis-b like virus (icron). | 1987 | 3623643 | |
| aflatoxin detected in human breast milk by immunoassay. | several epidemiological studies have shown a positive association between dietary exposure to aflatoxin (af) and an increased incidence of primary hepatocellular carcinoma (phc). one area in which little information is available is the exposure of newborn children to af in human breast milk. we report the development, validation and application of an enzyme-linked immunosorbent assay (elisa) to the detection of af in human breast milk. the assay allows the quantitation of 2 pg afm1 per ml of mil ... | 1987 | 3623716 |
| molecular cloning and sequencing of a human hepatitis delta (delta) virus rna. | human hepatitis delta (delta) virus (hdv) is a form of defective virus, which infects humans only in the presence of a co-infecting hepatitis b virus (hbv). hdv superinfection in a chronic hbv carrier often results in severe chronic hepatitis and cirrhosis, whereas acute hdv and hbv co-infection is frequently associated with fulminant hepatitis. hdv consists of a 36-nm particle, which contains an envelope with hbv surface antigen, and a nucleocapsid containing the hepatitis delta-antigen (hdag) ... | 1987 | 3627276 |
| a cultured cell receptor for the small s protein of hepatitis b virus. | human hepatitis b virus (hbv) has not been passaged in established cell culture systems. to determine whether this inability results from the lack of a receptor, 30 cell lines were examined for their abilities to bind 125i-labeled recombinant hepatitis b virus surface antigen (rhbsag) particles. these particles contained only the small surface (s) protein, which is also found in the envelope of infectious hbv particles. only two cells lines, both derived from african green monkey kidney, were ab ... | 1987 | 3629972 |
| selective synthesis and secretion of particles composed of the hepatitis b virus middle surface protein directed by a recombinant vaccinia virus: induction of antibodies to pre-s and s epitopes. | selective synthesis in mammalian cells of the hepatitis b virus middle surface (ms) protein, which is 55 amino acids longer than the major surface (s) protein, was achieved by using a recombinant vaccinia virus. the 33-kilodalton ms polypeptide was glycosylated and secreted as particles that resembled human hepatitis b surface antigen as well as particles composed solely of s protein with regard to antigenicity, buoyant density, size, and electron micrographic appearance. the ms particles differ ... | 1987 | 2434666 |
| a serologic follow-up of the 1942 epidemic of post-vaccination hepatitis in the united states army. | an epidemic of icteric hepatitis in 1942 affected approximately 50,000 u.s. army personnel. this outbreak was linked to specific lots of yellow-fever vaccine stabilized with human serum. to identify the responsible virus and the consequences of the epidemic, during 1985 we interviewed and serologically screened 597 veterans who had been in the army in 1942. these subjects were selected from three groups. group i consisted of patients who had received the implicated vaccine and had jaundice; grou ... | 1987 | 2436048 |
| yeast recombinant hepatitis b vaccine. | human hepatitis b virus vaccine was prepared using antigen produced by recombinant technology in yeast (saccharomyces cerevisiae). the highly purified antigen had the correct amino acid sequence and assumed the appropriate conformational structure to present the immunologic determinants (epitopes) that are needed to stimulate an appropriate immune response. yeast-derived vaccine, was safe and was equally immunogenic and protective against hepatitis b as plasma-derived vaccine, as demonstrated in ... | 1987 | 2437037 |
| presentation of two epitopes of the pres2 region of hepatitis b virus on live recombinant bacteria. | having developed a genetic procedure to expose a foreign epitope at the surface of escherichia coli by using the outer membrane lamb protein as a carrier, we apply this procedure to express two distinct portions of the pres2 region of hepatitis b virus: region a, residues 132-145, and region b, residues 153-171. the resulting hybrid proteins (lamb-pres2 a and lamb-pres2 b) were normally expressed, stable, and still kept most biologic functions of lamb. the corresponding bacterial strains were us ... | 1987 | 2442253 |
| detection of antibodies to pre-s2 encoded epitopes of hepatitis b virus by monoclonal antibody-enzyme immunoassay. | an inhibition enzyme immunoassay (ieia) for the detection of anti-pre-s2 antibody has been developed and used to evaluate anti-pre-s2 responses in the sera of patients recovering from acute type b hepatitis and in the sera of healthy recipients of hbv vaccine. in the assay, we used two monoclonal antibodies recognizing the nonoverlapping epitopes (pre-s2a and pre-s2b) of the pre-s2 protein of hbv envelope which compete with human anti-pre-s2 for the limited antibody-binding sites on recombinant ... | 1987 | 2442267 |
| characteristics of a cell line (tong/hcc) established from a human hepatocellular carcinoma. | a continuous adherent cell line was established from a hepatocellular carcinoma of an hbsag-positive italian male. this cell line, designated tong/hcc, has been grown in a hormone-supplemented medium for more than 18 months. the cell line secretes hbsag, alpha-fetoprotein, albumin and alpha 1-antitrypsin. alpha-fetoprotein production is enhanced by the addition of hydrocortisone and appears to be glucocorticoid concentration-dependent. the concentrates of the supernatant from the cell cultures a ... | 1987 | 2445644 |
| elevated hla class i antigen expression on peripheral blood mononuclear cells of hbsag carriers with coexistent human immunodeficiency virus infection. | the density of hla class i antigen on peripheral blood mononuclear cells was evaluated by flow cytometry in the following groups of patients: 41 hbsag carriers; 12 individuals with chronic non-a, non-b hepatitis, and 4 with acute hepatitis b. fourteen of the carriers were positive for antibody to human immunodeficiency virus, and all were negative for antibody to delta agent. elevated levels of class i antigen were observed in only 19% of patients with chronic hepatitis b virus infection alone. ... | 1987 | 2445645 |
| antigenic mapping of the surface proteins of infectious hepatitis b virus particles. | to identify further the surface proteins of the native virus, hepatitis b virus (hbv) particles purified from hbe antigen (ag)-positive human sera were used as immunogens to produce murine monoclonal antibody (mab)-secreting hybridomas. the specific binding of antibodies to the hbv envelope (env) proteins was determined in indirect radioimmunoassay and by western blot analysis. six mabs directed against major hepatitis b surface antigen (hbsag) recognized conformational epitopes on s proteins (p ... | 1987 | 2445903 |
| hbsag positive reactivity in man not due to hepatitis b virus. | a viral infection characterised by serum hbsag positivity with serum anti-hbc negativity has been encountered in senegal. the infection is not associated with the presence of hbeag, so it differs from hepatitis b virus in its core antigen, but the surface antigen of the two viruses share some epitopes. after the loss of hbsag, neither anti-hbc nor anti-hbs becomes detectable. anti-hbs, naturally acquired or produced by immunisation, does not protect against this new infection. chronic carriage o ... | 1987 | 2446096 |
| the isolation and purification of pre-s2 containing hepatitis b virus surface antigen by chemical affinity chromatography. | a simple, rapid, and efficient method was developed to isolate and purify pre-s2 containing hbsags from the plasma of a single chronic carrier of hbsag (adw) by ammonium sulfate fractionation, hydroxyapatite column chromatography, and polymerized human serum albumin-affinity column chromatography. about 500 micrograms of pre-s2 containing hbsag was obtained from 140 ml of plasma containing 4,200 micrograms of hbsag. two purified pre-s2 containing hbsags were analyzed by sds-polyacrylamide gel el ... | 1987 | 2840854 |
| functional characterization of cloned intrahepatic, hepatitis b virus nucleoprotein-specific helper t cell lines. | we have previously reported the establishment and preliminary characterization of polyclonal hepatitis b virus (hbv) nucleoprotein (hbcag)-specific cd4+ and cd8+ t cell lines derived from the hepatic lymphomononuclear cell infiltrate of several patients with chronic active hepatitis b. the isolated subsets from these lines were specifically activated by hbcag and displayed antigen-specific help and suppression with respect to proliferation of the alternate subset. one of these lines was recently ... | 1987 | 2439590 |
| adenine arabinoside monophosphate (vidarabine phosphate) in combination with human leukocyte interferon in the treatment of chronic hepatitis b. a randomized, double-blinded, placebo-controlled trial. | study objective: to determine the efficacy of adenine arabinoside monophosphate (ara-amp vidarabine phosphate) with or without human leukocyte interferon in chronic hepatitis b. study design: randomized, double-blinded, placebo-controlled trial with 6-month treatment and an 18-month follow-up. setting: referral-based liver-disease clinics at three university medical centers. patients: twenty-five patients with chronic active hepatitis or cirrhosis and 39 with chronic persistent hepatitis. interv ... | 1987 | 2441633 |
| receptors for polymerized human serum albumin in plasma derived hepatitis b vaccines. | the presence of polymerized human serum albumin receptors (phsa-r) in two hepatitis b virus (hbv) plasma derived vaccines (hb-vax, merck sharp and dohme; hevac-b, pasteur) was detected by three methods, using phsa polystyrene coated beads and 125i-anti-hbs (method 1) and polyclonal (method 2) or monoclonal (method 3) peroxidase conjugated anti-hbs. only a very weak reaction was found for phsa-r in hb-vax vaccine when the tests were performed in undiluted vaccine. no reactivity in 1/100 dilution ... | 1987 | 2829456 |
| review of the herpesviruses and hepatitis a: the potential hazards in dental care. | there is now an increased awareness of the risk of transmission of viral infections via blood and saliva after the publicity given to the human immunodeficiency viruses responsible for the acquired immunodeficiency syndrome. the herpesviruses have been found in blood, in blood products, and in saliva, and information with respect to these routes of transmission is convincing. although hepatitis a virus is spread predominantly by water contaminated with feces (fecal-oral route), the virus is also ... | 1987 | 2827085 |
| the mode of hepatitis b virus dna integration in chromosomes of human hepatocellular carcinoma. | nineteen dna samples that carry integrated hepatitis b virus (hbv) dna were isolated from seven independent human hepatomas by molecular cloning, and their structures were determined. the results, combined with reported data, were analyzed so that one can obtain insights into the mechanisms of integration of this virus dna and possible rearrangements that occur subsequently. the distribution of dna junctions along the virus genome suggests that there are recombination-proficient regions. thus, a ... | 1987 | 2828171 |
| production of hepatitis b virus surface antigen particles by the human hepatoma cell line hugk-14 in a serum-free medium. | the human hepatoma cell line, hugk-14, containing integrated hepatitis b virus (hbv) dnas, produced a large number of 23 nm spherical particles of hbv surface antigen (hbsag) in a serum-free medium. these particles closely resembled the hbsag particles found in the sera of hbv carriers and elicited a high antibody response in guinea pigs. the hugk-14 cells in a serum-free medium would provide a new, economic source of hbv vaccine. | 1987 | 2828295 |
| transformation of nih/3t3 cells by dna from a human hepatoma cell line with integrated hepatitis b virus dna. | we have studied by means of dna-mediated gene transfer the transforming activity of the dna of the human hepatoma cell line hcc-m, which contains genomes of hepatitis b virus (hbv) in integrated form. dna from hcc-m induced transformed foci on transfection of nih/3t3 cells. dnas from primary transformants were capable of inducing secondary transformants. most of the dnas of these transformants were demonstrated to contain both human repetitive sequences and hbv dna, indicating that the transform ... | 1987 | 2832175 |
| purification of hepatitis b virus gene x product synthesized in escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis b virus. | a fused gene containing 94% of the hepatitis b virus (hbv) open reading frame x was expressed in escherichia coli, and its 17-kda product was purified by ion-exchange chromatography. antibody elicited against the x-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing hbv particles. no such reaction was observed with the same cell line that did not produce hbv particles. | 1987 | 2832251 |
| inhibition of albumin binding to hepatitis b virions by monoclonal antibody to the pres2 domain of the viral envelope. | the binding of polyalbumin to hepatitis b virus (hbv)-associated envelope epitopes has been studied by means of a radioimmunoprecipitation technique. hbv particles were purified from the sera of chronic hepatitis b surface antigen (hbsag) carriers and labelled through the endogenous hbv-dna polymerase reaction. human albumin, polymerized through glutaraldehyde cross-linking, was able to precipitate (100%) labelled hbv at concentrations of 31.2 and 62.5 micrograms/ml, in contrast to monomeric alb ... | 1987 | 2452108 |
| [hepatitis-related viral markers in patients under long-term hemodialysis]. | antibody profiles for hepatitis b virus (hbv), hepatitis a virus (hav), cytomegalovirus (cmv), epstein-barr virus (ebv) and human immunodeficiency virus (hiv) were determined on 55 serum samples collected from patients with chronic renal failure who were on long-term haemodialysis for periods ranging from 8 months to 5 years and 3 months. the exposure rates for hbv, hav, cmv, ebv and hiv were 94.5%, 100%, 94.5%, 94.5% and 0% respectively. among the 7 hbsag carriers, 1 and 3 were positive for e a ... | 1987 | 2452721 |
| conjugates of ara-amp with lactosaminated albumin: a study on their immunogenicity in mouse and rat. | complexes of albumin with oligosaccharides have been successfully employed in experimental chemotherapy as hepatotropic carriers of antiviral drugs or as vectors of anticancer-agents, but their clinical use is hampered by an immune response they might evoke. in the present experiments we have studied the humoral immunogenicity of conjugates of 9-beta-d-arabinofuranosyl adenine 5'-monophosphate (ara-amp) with lactosaminated albumin (l-sa), in mice and rats. these complexes were prepared with the ... | 1987 | 2453263 |