Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| nucleic acid from an adeno-associated virus: chemical and physical studies. | 1966 | 5229859 | |
| replication of an adeno-associated virus in canine and human cells with infectious canine hepatitis virus as a "helper". | 1967 | 4318961 | |
| studies on the relationship between adeno-associated virus type i (aav-1) and adenoviruses. i. replication of aav-1 in certain cell cultures and its effect on helper adenovirus. | 1967 | 4290509 | |
| studies on the relationship between adeno-associated virus type 1 (aav-1) and adenoviruses. ii. inhibition of adenovirus plaques by aav; its nature and specificity. | 1967 | 4964865 | |
| adeno-associated virus studies employing a fluorescent focus assay technique. | 1967 | 15938292 | |
| oncogenicity of mixtures of adeno-associated virus and adenovirus type 12. | 1968 | 4302692 | |
| oncogenicity of mixtures of adeno-associated virus and adenovirus type 12. | 1968 | 5659627 | |
| inhibition of adenovirus 12 oncogenicity by adeno-associated virus. | 1968 | 5668107 | |
| haemagglutinating adeno-associated virus (aav) in association with bovine adenovirus type 1. brief report. | 1970 | 5530958 | |
| the potentiation of type 1 adeno-associated virus by temperature-sensitive conditional-lethal mutants of celo virus at the restrictive temperature. | 1971 | 5000131 | |
| electron microscopy of dna from adeno-associated virus type i. | 1971 | 4101807 | |
| separation of two types of adeno-associated virus particles containing complementary polynucleotide chains. | an adeno-associated virus containing bromodeoxyuridine-substituted deoxyribonucleic acid has been fractionated by equilibrium centrifugation in cscl into two classes of virions which contain complementary polynucleotide chains. | 1972 | 5014934 |
| detection and localization of viruses in human fetal intestinal organ cultures by immunofluorescence. | viral antigens from viruses belonging to four different viral groups were detected directly in human fetal intestinal organ cultures by the application of immunofluorescent techniques. the time of appearance and the cellular localization of fluorescent-stainable antigen varied with the type of virus under investigation. after infection with adenovirus or with adeno-associated virus, fluorescent-stainable antigen was seen in the epithelial cells of the explants, though no light microscopic change ... | 1972 | 4344634 |
| arrangement of nucleotide sequences in adeno-associated virus dna. | 1973 | 4586409 | |
| letter: visualization of the inverted terminal repetition in adeno-associated virus dna. | 1974 | 4816655 | |
| study of the fine structure of adeno-associated virus dna with bacterial restriction endonucleases. | a physical map of the adeno-associated virus type 2 genome has been constructed on the basis of the five fragments produced by the restriction endonucleases hindii + iii from hemophilus influenzae. there are three endo r-hindii cleavage sites and one endo r-hindiii site. evidence has been obtained to support the existence of two nucleotide sequence permutations in adeno-associated virus dna, the start points of which have been estimated to be separated by 1% of the genome. the three cleavage fra ... | 1975 | 1159899 |
| [study of the possibilities of inactivating adeno-associated virus type 4]. | adeno-associated type 4 virus (aav-4) is inactivated by formalin dilutions 1 : 400, 1 : 1000 and 1 : 2000 for 24 hours at 37 degrees c. under these conditions hemogglutinins (ha) and complement-fixing antigen (cfa) are retained. the residual formalin must be neutralized with sodium bisulphite. aav-4 can also be inactivated by 3% hydrogen peroxide and ha and cfa are retained too. hydrogen peroxide may be neutralized with catalase. no inactivation of aav-4 could be achieved with lowere concentrati ... | 1975 | 1162954 |
| detection of adeno-associated virus (aav)-specific nucleotide sequences in dna isolated from latently infected detroit 6 cells. | 1975 | 1198930 | |
| complementation of adeno-associated virus growth with temperature-sensitive mutants of human adenovirus types 12 and 5. | temperature-sensitive mutants of human adenovirus types 12 and 5, defective in viral dna synthesis, were able to support growth of adeno-associated virus type i at the non-permissive temperature. | 1975 | 213533 |
| adeno-associated virus in adenovirus type 3 conjunctivitis. | although human infection with adenovirus-associated virus (aav) has been demonstrated, there is no evidence that disease results from such infections. the proportion of adenovirus infections which are dual infections with aav is virtually unknown, since special methods are required to demonstrate infection with aav. to search for aav, we re-examined a collection of specimens from 40 persons involved in an epidemic of pharyngoconjunctival fever associated with a swimming pool. virological and ser ... | 1975 | 166918 |
| effect of adeno-associated virus on cancer expression by herpesvirus-transformed hamster cells. | infection of herpes simplex virus type-2-transformed hamster tumor cells with adeno-associated virus type 1 before inoculation into hamsters specifically delayed the appearance of palpable tumors and increased the survival time of the animals. the data indicated that a defective virus of humans can influence cancer expression by a virus-transformed cell. | 1975 | 171433 |
| detection and serological identification of adeno-associated virus in avian adenovirus stocks. | eleven avian adenovirus strains were tested for the presence of avian adeno-associated viruses (aaav). six strains contained aaav. electron microscopy using rabbit anti-aaav serum was useful in detecting the satellite virus. the aaav previously isolated from guail bronchitis virus was related to each of the six new isolates by immunoagglutination, complement fixation, immunodiffusion, and neutralization tests. | 1975 | 803468 |
| [development of a method for preparing adeno-associated virus type 4 antigen]. | a method for preparation of adeno-associated type 4 virus (aav-4) purified from group-specific adenovirus antigen by adsorption on formalinized sheep erythrocytes and elution into hypertonic nacl solution was developed. in 1 m nacl solution the purified aav-4 retained its infectivity and the complement-fixing and hemagglutinating activities. separation of aav-4 and adenovirus group-specific complement-fixing antigen was based on differences in conditions of their adsorption and elution. aav-4 wa ... | 1976 | 1258451 |
| the presence of avian adenoviruses and adeno-associated viruses in healthy chickens. | avian adenoviruses were isolated from 56 of 106 fecal and rectal tissue samples taken from apparently healthy young chickens on 4 farms. only one isolation was made from the 37 samples from 3- and 4-week-old chicks, while the isolation frequency was 64-100% in groups 5 weeks old and older. the 56 adenovirus isolates were classified into 6 serotypes. vurses of 3 or 4 types were found on each farm. avian adeno-associated virus cf antigens were found, using chicken embryos coinfected with an adenov ... | 1976 | 1259656 |
| multiple structures of adeno-associated virus dna: analysis of terminally labeled molecules with endonuclease r-hae iii. | the double-stranded form of adeno-associated virus (aav) dna has about 20 sites sensitive to endonuclease r.hae iii from haemophilus aegypitus; the fragments produced fall into about 13 size classes, 8 of which contain single fragments. the location of the hae iii-produced aav fragments relative to the three ecor1 fragments was determined. using revised figures for the molecular weights of the hae iii cleavage products of phix174 replicative form dna, we calculated that aav dna contains about 4, ... | 1976 | 1271522 |
| intracellular distribution and polyadenylate content of adeno-associated virus rna sequences. | 1976 | 960561 | |
| genome localization of adeno-associated virus rna. | in previous work, linear duplex molecules of adeno-associated virus, type 2 (aav2), dna were cleaved with the restriction endonucleases r-ecori, r-hindii, and r-hindiii. the physical order of the specific fragments obtained was deduced and oriented with respect to the dna strand polarity and the direction of transcription. stable aav rna is transcribed only from 70% of the minus dna strand. we report here rna-dna hybridization experiments using these restriction fragments to obtain a more accura ... | 1976 | 972429 |
| multiplication of adeno-associated virus type 1 in cells coinfected with a temperature-sensitive mutant of human adenovirus type 31. | 1976 | 982809 | |
| isolation and characterization of adeno-associated viruses from bovine adenovirus types 1 and 2. | hemagglutinating dna viruses of 20 nm diameter were isolated from bovine adenovirus types 1 and 2. the isolates were heat stable, chloroform resistant, and defective. their densities were 1.38 to 1.39 g/cm3, and they were found to be serologically identical to the bovine adeno-associated virus strain x7. a partial antigenic relationship was found between these and the canine adeno-associated virus. | 1976 | 986122 |
| cleavage of adeno-associated virus dna with sali,psti and haeii restriction endonucleases. | duplex aav-2 dna was digested with sali, psti or haeii restriction endonucleases and the cleavage sites were mapped. sali cleaves aav dna at 0.310 map units, psti at 0.106, 0.422 and 0.914 and the five haeii sites were mapped at 0.110. 0.156, 0.181, 0.536 and 0.600 map units. these cleavage products will be useful for the isolation of specific regions from the aav dna, located outside of the stably transcribed region of the genome, and will also help to map more complex restriction enzyme cleava ... | 1976 | 995645 |
| helper factor(s) for growth of adeno-associated virus in cells transformed by adenovirus 12. | evidence is presented that a helper factor(s) for growth of adeno-associated virus (aav) is present in cells transformed by adenovirus type 12 (ad12). the growth of aav was observed in heterokaryons formed by fusion of human kb and ad12-transformed rodent cells by using ultraviolet-inactivated sendai virus without coinfection of cells with adenovirus. the presence of the helper factor(s) for aav growth in rat cells transformed by the ecori-c fragment or the hindiii-g fragment of ad12dna suggests ... | 1977 | 337298 |
| viral dna synthesis in vitro with nuclei isolated from adeno-associated virus type 1-infected cells. | 1977 | 841869 | |
| origin and termination of adeno-associated virus dna replication. | 1977 | 867815 | |
| establishment and characterization of kb cell lines latently infected with adeno-associated virus type 1. | 1977 | 898681 | |
| isolation of the viral dna replication complex from adeno-associated virus type 1-infected cells. | the replication complex active in adeno-associated virus type 1 (aav-1) dna synthesis in vitro was solubilized, with a nonionic detergent, from the nuclei of human embryonic kidney cells coinfected with aav-1 and an early temperative-sensitive mutant (ts125) of human adenovirus type 5 at the nonpermissive temperature (40.5 degrees c). the complex sedimented with a mean size of 23s and contained parental aav-1 dna. most of the dna synthesized with the aav-1 dna replication complex in vitro was aa ... | 1977 | 916024 |
| evidence for two nucleotide sequence orientations within the terminal repetition of adeno-associated virus dna. | duplex adeno-associated virus (aav) dna, produced by annealing plus and minus virion single strands, has been digested with several bacterial restriction endonucleases. these studies reveal the existence of alternate secondary structures at the termini of duplex aav dna. analysis of the sites of endo r-hpa ii cleavage, the products of complete endo r-hpa ii digestion, and the multiple terminal secondary structures leads to the conclusion that there are two possible nucleotide sequences at each e ... | 1977 | 916029 |
| adeno-associated virus dna structure. restriction endonuclease maps and arrangement of terminal sequences. | 1977 | 919346 | |
| structure and nucleotide sequence of the terminal regions of adeno-associated virus dna. | 1977 | 194395 | |
| serological response of chickens exposed to a type 1 avian adenovirus alone or in combination with the adeno-associated virus. | the avian adenoviruses (av) are common infectious agents of poultry and other avian species throughout the world (1,4,8). limited observations suggest that the adeno-associated virus (a-av) coinfects many of the chickens that carry av (8). the presence and persistence of these infections in a flock is often determined by serological methods. in the current study, the immune response of chickens to type 1 av alone and to a dual exposure, av plus a-av, was followed over a 12-week period with a var ... | 1977 | 199153 |
| effect of avian adeno-associated virus on pathogenicity of tipton virus in chicks. | the avian adeno-associated virus (a-av) reduced the pathogenicity of an adenovirus infection in vivo. groups of chicks were infected with tipton virus alone or in combination with high or low doses of a-av. in both trials, the associated virus delayed and reduced chick mortality. this effect was dose-dependent and significant at the higher dose level. | 1978 | 209781 |
| [helper viruses of adeno-associated virus type 4 replication]. | in replication of adeno-associated virus type 4 (aav-4) the helper function may be performed by a non-defective virus from the same group of parvoviruses (kilham virus). the synthesis of aav-4 antigen was observed in a pig embryo kidney cell line, spev, chronically infected with kilham virus, strain rv-13, 45--52 passages. a one-day-old spev-kilham culture was infected with aav-4. the aav-4 antigen was detected by immunofluorescence at 6, 8, 12, 18 hours, 2, 3, 4, and 5 days after inoculation. d ... | 1979 | 217178 |
| parvovirus rna transcripts containing sequences not present in mature mrna: a method for isolation of putative mrna precursor sequences. | we report here a method of rna preparation that may enrich for precursor rna sequences and the results of an investigation of adeno-associated virus (aav) rna transcription that used this method. whole cells were lysed with detergent and high salt and separated into supernatant and pellet (crude chromatin) fractions. these fractions were then separately deproteinized by proteolytic digestion and phenol extractions. dna was removed from the preparation by two cycles of pancreatic dnase digestion ... | 1979 | 218213 |
| adeno-associated virus autointerference. | 1979 | 218354 | |
| adeno-associated virus dna replication: nonunit-length molecules. | 1979 | 219605 | |
| defective-interfering particles of the human parvovirus adeno-associated virus. | 1979 | 220782 | |
| adeno-associated virus dna replication complexes in herpes simplex virus or adenovirus-infected cells. | a complex which is active in in vitro synthesis of adeno-associated virus (aav) dna was solubilized from vero cells that were co-infected with aav and either adenovirus (ad5) or a herpes simplex virus type 1 (hsv-1) as the helper virus. the complexes from the ad5 and hsv-1-infected cells sedimented at 23 s and 28 s, respectively. the optimal conditions for in vitro dna synthesis for the two types of complex using the endogenous aav template and the endogenous dna polymerase, differed with respec ... | 1979 | 221504 |
| studies on the defectiveness of adeno-associated virus (aav). i. effects of phosphonoacetic acid and 2-deoxy-d-glucose on the replication of aav. | 1979 | 222062 | |
| studies on the defectiveness of adeno-associated virus (aav). ii. effect of growth in cv-1 cells on the replication of adeno-associated virus. | 1979 | 222063 | |
| isolation of an adeno associated virus from sheep. brief report. | an adenovirus and a spherical virus 20--24 nm diameter were isolated from ovine faeces. the small virus replicated in the nucleus, and was associated especially with the nucleolus. it haemagglutinated guinea pig and human erythrocytes, was thermostable and required an adenovirus for replication. it is concluded that this represents the first recorded isolate of an ovine aav. | 1979 | 226038 |
| adeno-associated virus dna replication. | 1979 | 226321 | |
| adeno-associated virus--an extreme state of viral defectiveness. | 1979 | 228349 | |
| molecular structure of adeno-associated virus variant dna. | when lysates of human cells, infected jointly with the defective parvovirus, adeno-associated virus (aav), and a helper adenovirus, are banded to equilibrium in cscl buoyant density gradients, virus particles of various densities are obtained. infectious aav particles mainly band at a density of 1.41 g/cm3 with a minor component at 1.45 g/cm3. noninfectious aav particles band at densities between 1.41 and 1.32 g/cm3. we have analyzed, by mapping with site-specific endodeoxyribonucleases, the mol ... | 1980 | 6244311 |
| heavy and light particles of adeno-associated virus. | kb cells coinfected with adenovirus and adeno-associated virus (aav) yielded two kinds of infectious aav particles that banded in cscl at densities of 1.45 and 1.41 g/cm(2), respectively. the 1.45 band was found to be composed of a heterogeneous group of viral particles that could be subfractionated by velocity sedimentation. the main component from this band had a smaller s value (109) than the main component from the 1.41 band (111s), although both had the same dna/protein ratio and the same d ... | 1980 | 6245263 |
| assembly of adeno-associated virus. | 1980 | 6245509 | |
| nucleotide sequence of the inverted terminal repetition in adeno-associated virus dna. | the inverted terminal repetition in adeno-associated virus type 2 dna has been sequenced. the terminal repetition contain 145 nucleotides of which the first 125 nucleotides can self-base pair to form a t-shaped hairpin structure. both restriction endonuclease analysis with smai and bgli and direct sequence analysis of the smai fragments provide evidence for two sequences in the region of the terminal repetition between nucleotides 44 and 81. the two sequences represent an inversion of the first ... | 1980 | 6246271 |
| adenovirus early region 1b gene function required for rescue of latent adeno-associated virus. | 1980 | 6249041 | |
| integration of the adeno-associated virus genome into cellular dna in latently infected human detroit 6 cells. | a clone of human cells (detroit 6) latently infected by adeno-associated virus (aav) has been characterized with regard to the status of the viral dna. in both early (9 to 10) and late (118) passages of the clone, aav-dna was recombined with host dna, at least in some cases as a head-to-tail tandem repeat, via the terminal sequences of the viral genome. however, it was not possible to distinguish between integration into chromosomal dna and very large plasmids (< 20 x 10(6) molecular weight) whi ... | 1980 | 6251245 |
| adenovirus helper function for growth of adeno-associated virus: effect of temperature-sensitive mutations in adenovirus early gene region 2. | adeno-associated virus (aav) grows efficiently only in cells that are also infected with an adenovirus (ad). we employed ad mutants to determine which genes may be required for the aav helper function. two mutants of ad type 5 (ad5), ad5ts125 and ad5ts107, with temperature-sensitive lesions in the e72 dna-binding protein coded by the ad early region 2, were deficient for aav helper functions at the nonpermissive temperature (40 degrees c). in contrast, ad5ts149, with a temperature-sensitive lesi ... | 1980 | 6251278 |
| physical mapping of adeno-associated virus serotype 4 dna. | the organization of adeno-associated virus serotype 4 (aav 4) dna was probed by using restriction enzymes. the cleavage sites of the following enzymes were mapped: bglii, bami, hincii, kpni, psti, sali, ssti, and xhoi. the orientation of transcription on the physical cleavage map was determined by locating the fragments which contain the 3' end of the mrna. strand separation gels were run for hinii fragments of aav 4 dna. by hybridizing aav 4 mrna to the resolved strands, the polarity of the dna ... | 1980 | 6252330 |
| characterization of aleutian disease virus as a parvovirus. | we characterized a strain of aleutian disease virus adapted to growth in crandall feline kidney cells at 31.8 degrees c. when purified from infected cells, aleutian disease virus had a density in cscl of 1.42 to 1.44 g/ml and was 24 to 26 nm in diameter. [3h]thymidine could be incorporated into the viral genome, and the viral dna was then studied. in alkaline sucrose gradients, aleutian disease virus dna was a single species that cosedimented at 15.5s with single-stranded dna from adeno-associat ... | 1980 | 6252342 |
| enhancement and inhibition of celo virus pathogenicity in quail by avian adenovirus-associated virus. | dual infection of 12 day-old quail (colinus virginianus) with 10(6) plaque forming units of celo virus and low doses of avian adeno-associated virus (a-av), resulted in significant enhancement of celo virus-induced mortality, whereas dual infections with high doses of a-av resulted in a delay in mortality. a-av induced enhancement and inhibition of celo virus pathogenicity could be blocked by the addition of a-av antiserum prior to infection. | 1980 | 6253685 |
| transcripts of the adeno-associated virus genome: mapping of the major rnas. | the four major adeno-associated virus type 2 (aav2)-specific rnas were mapped on the linear viral genome by a variety of biochemical techniques, including s1 nuclease and exonuclease vii mapping, rna gel-transfer hybridization, and analysis of reverse transcriptase extension products. all the major aav2 rnas were derived from the minus dna strand and had 3' termini at position 96. the nucleus-specific 4.3- and 3.6-kilobase (kb) rnas had 5' termini at positions 6 and 19, respectively. the 5' term ... | 1980 | 6255215 |
| properties of adeno-associated virus (type 1) replicated in rodent cells by murine adenovirus. | we report for the first time the replication of infectious adeno-associated virus type 1 (aav-1) in rodent cells [primary mouse kidney (pmk) and mouse l929 cells] using murine adenovirus (mav) as a helper virus and also the production of aav-i virus antigen by herpes simplex virus type i (hsv-i) with its temperature-sensitive mutant ts 200 in mouse neuroblastoma (nb) cells. the infectious aav virions produced by mav on l cells had a buoyant density of 1.41 2ml in caesium chloride gradients. | 1980 | 6257836 |
| [significance of avian adeno-associated virus (aaav) in serological studies of adenovirus infections in chickens]. | 1980 | 6258360 | |
| transplacental infection with adeno-associated virus type 1 in mice. | adeno-associated type 1 parvovirus (aav) was detected in the kidneys and lungs of fetuses and newborns, when pregnant mice were injected subcutaneously with aav type 1 and murine adenovirus as a helper virus. these findings clearly indicate that transplacental infection with aav in rodents has been achieved. | 1980 | 6259087 |
| serologic evidence of avian adeno-associated virus infection in an unselected human population and among poultry workers. | six (6.0%) of 100 serum samples from an unselected adult population were positive for antibody to avian adeno-associated virus (a-av) by agar gel precipitation (agp), and 10 (15.6%) of 64 were positive by virus neutralization (vn). three (14.3%) of 21 samples from poultry workers (industry or research) were positive for avian a-av antibody by agp and 14 (66.7%) of 21 were positive by vn. all sera positive by agp were also positive by vn. twenty-two of 244 sera positive for antibody to avian a-av ... | 1981 | 6261579 |
| growth of adeno-associated virus in embryonated eggs. | 1981 | 6262350 | |
| inverted terminal repetition in adeno-associated virus dna: independence of the orientation at either end of the genome. | complementary strands of adeno-associated virus dna labeled with 32p at the 5' ends were separated and then self-annealed to form single-stranded circles stabilized by hydrogen bonds between the complementary sequences in the inverted terminal repetitions. we have previously shown that there are two distinct sequences in the terminal repetition which represent an inversion of the first 125 nucleotides (e. lusby et al., j. virol. 34:402-409, 1980; i. s. spear et al., virology 24:627-634, 1977). b ... | 1981 | 6262528 |
| eukaryotic translational control: adeno-associated virus protein synthesis is affected by a mutation in the adenovirus dna-binding protein. | growth of adeno-associated virus (aav) requires expression of certain adenovirus (ad) genes in the same cell. aav particles contain three proteins, vp1 (mr 85,700), vp2 (mr 72,000), and vp3 (mr 61,500). these proteins have overlapping peptide maps. we recently reported that aav rnas make up a 3'-coterminal family of overlapping molecules. we report here that the most abundant aav mrna, a 2.3-kilobase spliced rna, codes for all three proteins--vp1, vp2, and vp3--when translated in an in vitro ret ... | 1981 | 6265925 |
| [experimental infection of green monkeys with adenoassociated virus]. | primary infection and reinfection with adeno-associated virus type 4 (aav-4) was reproduced in green monkeys experimentally infected with aav-4 in mixture with adenovirus. wide dissemination of the satellite virus in animals was observed. aav-4 and its antigen were detectable 5 to 23 days after inoculation. in monkeys infected with a mixture of aav-4 and adenovirus or with one of them the infection was accompanied by a marked fever persisting from the 5th to the 20th day after inoculation. the i ... | 1981 | 6266159 |
| [isolation of adeno-associated virus type 4 from a culture of green monkey kidney cells]. | in the process of biological control of uninfected green monkey kidney (gmk) cell cultures a thermostable hemagglutinating agent designated no. 5056 was isolated alongside with adenovirus-sv. by its antigenic properties the 5056 strain was identified as adeno-associated virus type 4 (aav-4). in control of 574 specimens of gmk culture batches, 40 aav-4 strains were isolated in the presence of a helper adenovirus. some biological properties of the isolates and their resistance to certain physical ... | 1981 | 6266160 |
| inhibition of adenovirus-transformed cell oncogenicity by adeno-associated virus. | 1981 | 6267797 | |
| inhibition of adenovirus oncogenicity in hamsters by adeno-associated virus dna. | adeno-associated virus (aav) dna partially inhibited in syrian golden hamsters the formation of tumors by the human adenovirus (ad) type 12. furthermore, defective interfering aav particles or their dna also reduced tumorigenesis. defective aav particles contain aberrant genomes with extensive deletions of the internal aav dna sequences. variant aav dna, containing 30% of the viral genome, decreased the incidence of ad-induced tumors from 44 to 18%. defective aav particles, which have a buoyant ... | 1981 | 6273635 |
| a cascade of adenovirus early functions is required for expression of adeno-associated virus. | one measurable biological activity of early adenovirus genes is their ability to promote growth of the defective adeno-associated virus, aav. we have identified an ordered sequence of communications among the early genes of adenovirus type 2 (ad2) that results in expression of the helper activity. we purified dna fragments and mrnas corresponding to early ad2 regions e1, e2a, e3 and e4 and injected them via glass capillaries into aav-infected cells. dnas were placed in the nucleus, mrnas in the ... | 1981 | 6276019 |
| adeno-associated virus rna transcription in vivo. | we have studied rna transcripts of the defective parvovirus, adeno-associated virus (aav) present in poly(a)rich and poly-(a)free fractions of nuclear and cytoplasmic rna prepared from cells infected together with a helper adenovirus. cytoplasmic poly-(a)rich rna contains three overlapping spliced aav rnas having sizes of 3.9 x 10(3), 3.3 x 10(3) and 2.3 x 10(3) bases respectively. the nuclear precursors of these rnas appear to be the coterminal unspliced poly(a)-rich rnas containing 4.2 x 10(3) ... | 1981 | 6173214 |
| adeno-associated virus replication. the effect of l-canavanine or a helper virus mutation on accumulation of viral capsids and progeny single-stranded dna. | we have studied the relationship between adeno-associated virus (aav) dna replication and virus particle assembly. formation of empty or full particles and accumulation of aav capsid proteins was prevented in the presence of the arginine analogue, l-canavanine, or when a temperature-sensitive helper adenovirus was used at the nonpermissive temperature. in each case there was a concomitant inhibition of aav single-stranded (progeny) dna accumulation but little or no effect upon synthesis of aav d ... | 1981 | 6256359 |
| virological studies in amyotrophic lateral sclerosis. | complement-fixing antibody response to eleven different viruses were measured in amyotrophic lateral sclerosis (als) patients, contacts of als patients, neurological controls, and normal controls. the normal controls showed a decreased response to adeno-associated virus and an increased response to adenovirus when compared to the other groups. levels of interferon-like substances also were investigated in sera and cerebrospinal fluids of als patients and neurological controls. responses were of ... | 1982 | 6175901 |
| mapping of the 5' termini of two adeno-associated virus 2 rnas in the left half of the genome. | the left 45% of the adeno-associated virus 2 genome was sequenced. the 5' termini of two adeno-associated virus-specific rnas were mapped at the nucleotide level within this region of the genome (nucleotides 286 to 288 and 871 to 874). both of these 5' termini map 31 +/- 2 nucleotides downstream from the start of "tata' boxes. | 1982 | 6281463 |
| cloning of adeno-associated virus into pbr322: rescue of intact virus from the recombinant plasmid in human cells. | we have cloned intact duplex adeno-associated virus (aav) dna into the bacterial plasmid pbr322. the aav genome could be rescued from the recombinant plasmid by transfection of the plasmid dna into human cells with adenovirus 5 as helper. the efficiency of rescue from the plasmid was sufficiently high to produce yields of aav dna comparable to those observed after transfection with equal amounts of purified virion dna. thus, the recombinant plasmid itself may be a model for studying the rescue o ... | 1982 | 6281795 |
| effect of deletions in adenovirus early region 1 genes upon replication of adeno-associated virus. | the growth of adeno-associated virus (aav) is dependent upon helper functions provided by adenovirus. we investigated the role of adenovirus early gene region 1 in the aav helper function by using six adenovirus type 5 (ad5) host range mutants having deletions in early region 1. these mutants do not grow in human kb cells but are complemented by and grow in a line of adenovirus-transformed human embryonic kidney cells (293 cells); 293 cells contain and express the ad5 early region 1 genes. mutan ... | 1982 | 6284977 |
| is avian adeno-associated virus an endogenous virus of chicken cells? | the adeno-associated viruses (aav) are defective parvoviruses which produce infective progeny only in cells co-infected with a 'helper' adenovirus (ad). both human and simian aav have been recovered from human and simian primary cell cultures following their inoculation with 'aav-free' ad. whereas some studies have suggested that aav exists in a latent state in these cells, others have indicated that the aav genome is capable of establishing and maintaining a latent state in defined laboratory c ... | 1982 | 6285201 |
| defective parvoviruses acquired via the transplacental route protect mice against lethal adenovirus infection. | adeno-associated virus type 1 (aav-1) interfered with the replication of its murine adenovirus (mav) helper in primary mouse kidney cells and in 1-day-old icr mice. mice carrying aav-1 acquired via the transplacental route were protected against lethal infection with mav. the replication of aav-1 in these mice could be triggered by multiple challenges with mav, and antibodies to aav-1 were subsequently detected. | 1982 | 6286489 |
| characterization of heavy particles of adeno-associated virus type 1. | the temperature-sensitive mutant ts4 of adenovirus type 2 (ad-2) is capable of complementing adeno-associated virus type 1 (aav-1) in hep2, kb and hek cells at 34 degrees c and 39 degrees c when used as a helper virus. heavy non-infectious aav-1 particles can be generated by using the mutant ts4 in hep2 cells. when aav-1 is grown in serial passages in hep2 cells, both the wild-type ad-2 and the mutant ts4 give rise to heavy, less infectious aav-1 particles. the heavy aav-1 particles generated by ... | 1982 | 6292346 |
| adeno-associated virus helper activity of adenovirus dna binding protein. | the requirement for the adenovirus (ad) single-stranded dna binding protein (dbp) in the expression of adeno-associated virus (aav) proteins was studied by specific immunofluorescent staining of infected cells and in vitro translation of rna from infected cells. the ad5 mutant ts125, which carries a mutation in the dbp gene, helped aav as efficiently as the ad5 wild type (wt) did at both the permissive (32 degrees c) and nonpermissive (40.5 degrees c) temperatures in hela and kb cells. furthermo ... | 1982 | 6292524 |
| nucleotide sequence and organization of the adeno-associated virus 2 genome. | the complete nucleotide sequence of the adeno-associated virus 2 genome was determined. the single-stranded genome is 4,675 nucleotides in length and contains inverted terminal repeats of 145 nucleotides, the first 125 nucleotides of which form a palindromic sequence. within the inverted terminal repetitions, there are two distinct sequences representing an inversion of 43 nucleotides that can exist on either terminus. the 5' and 3' termini of three major mrna transcripts, which are present in b ... | 1983 | 6300419 |
| properties of an adenovirus type 2 mutant, ad2dl807, having a deletion near the right-hand genome terminus: failure to help aav replication. | we have analyzed the ability of an adenovirus type 2 mutant, ad2dl807, to support replication of adeno-associated virus (aav). this mutant has a deletion extending from early region 3 through the late fiber gene and into early region 4. since aav growth does not require ad early region 3 or the fiber gene, the ad2dl807 mutant allows analysis of the function of ad early region 4 in aav growth. as determined by assay of aav dna, rna, and protein synthesis as well as the number of cells producing a ... | 1983 | 6305001 |
| chromatin-like structure of adeno-associated virus dna in infected cells. | adeno-associated virus (aav) is a single-stranded dna virus which requires adenovirus as a helper for productive infection. we studied whether intracellular aav dna in kb cells was present in a chromatin-like structure by digesting infected cell nuclei with micrococcal nuclease. virus dna was detected by agarose gel electrophoresis followed by blotting and hybridization to nick-translated [32p]dna probes. after coinfection with adenovirus, aav dna was present in nucleosome-like structures which ... | 1983 | 6310160 |
| cloning of infectious adeno-associated virus genomes in bacterial plasmids. | we describe the construction of two escherichia coli hybrid plasmids, each of which contains the entire 4.7-kb dna genome of the human parvovirus, adeno-associated virus (aav) type 2. because the aav genome was inserted into the plasmid dna using bglii linkers the entire virus genome can be recovered by in vitro cleavage of the purified recombinant plasmid. transfection of these recombinant dnas into an adenovirus-transformed human cell line in the presence of helper adenovirus resulted in effic ... | 1983 | 6352411 |
| rescue of adeno-associated virus from recombinant plasmids: gene correction within the terminal repeats of aav. | we have isolated three types of pbr322-aav recombinant plasmids that contain deletions within the 145 bp aav terminal repeats. when the plasmids were transfected into human cells, mutants that contained deletions within the left (type i) or right (type ii) terminal repeat were viable. of four mutants examined that contained deletions in both termini (type iii), only one was viable. all of the viable mutants produced aav virions that contained wild-type aav dna. furthermore, the viable type iii d ... | 1983 | 6088052 |
| genetic analysis of adeno-associated virus: properties of deletion mutants constructed in vitro and evidence for an adeno-associated virus replication function. | transfection of a pbr322-based, recombinant plasmid, pav2, containing the entire adeno-associated virus (aav) type 2 genome into human 293 cells in the presence of helper adenovirus resulted in rescue and replication of aav to yield infectious particles. we constructed mutants of pav2 containing deletions within the aav sequence. we describe here the phenotypes of these aav deletion mutants. the results can be summarized as follows. mutants (cap-) with deletions between map positions 53 and 85 d ... | 1984 | 6088786 |
| adeno-associated virus proteins: origin of the capsid components. | the three primary capsid proteins (a, b, and c) of adeno-associated viruses have been shown previously to contain overlapping amino acid sequences (r. mcpherson and j. rose, j. virol. 46:523-529, 1983). in the present study we demonstrate definitively that these proteins are encoded in the right half of the adeno-associated virus 2 genome, and one or both of the smallest adeno-associated rna species (2.3- or 2.6-kilobase rna) account for their synthesis. protein a (90 kilodaltons) apparently ini ... | 1984 | 6092680 |
| use of adeno-associated virus as a mammalian dna cloning vector: transduction of neomycin resistance into mammalian tissue culture cells. | adeno-associated virus (aav) is a human dna virus that has a broad host range and can be grown both as an integrated provirus and as a lytic genome. these properties suggested that aav may be useful as a mammalian transduction vector. to test this possibility, we have isolated a recombinant aav viral stock in which the neomycin resistance gene was substituted for the aav capsid genes. using this recombinant stock, we have demonstrated that aav can be used to transduce foreign dna into human and ... | 1984 | 6093102 |
| replication of adeno-associated virus dna. complementation of naturally occurring rep- mutants by a wild-type genome or an ori- mutant and correction of terminal palindrome deletions. | when the entire adeno-associated virus (aav) genome is inserted into a bacterial plasmid, infectious aav genomes can be rescued and replicated when the recombinant aav-plasmid dna is transfected into human 293 cells together with helper adenovirus particles. we have taken advantage of this experimental system to analyze the effects of several classes of mutations on replication of aav dna. we obtained aav mutants by molecular cloning in bacterial plasmids of naturally occurring aav variant or de ... | 1984 | 6094825 |
| a human parvovirus, adeno-associated virus, as a eucaryotic vector: transient expression and encapsidation of the procaryotic gene for chloramphenicol acetyltransferase. | we have used the defective human parvovirus adeno-associated virus (aav) as a novel eucaryotic vector (parvector) for the expression of a foreign gene in human cells. the recombinant, pav2, contains the aav genome in a pbr322-derived bacterial plasmid. when pav2 is transfected into human cells together with helper adenovirus particles, the aav genome is rescued from the recombinant plasmid and replicated to produce infectious aav particles at high efficiency. to create a vector, we inserted a pr ... | 1984 | 6095038 |
| the genetics of adeno-associated virus. | 1984 | 6098150 | |
| genetics of adeno-associated virus: isolation and preliminary characterization of adeno-associated virus type 2 mutants. | we constructed insertion and deletion mutants with mutations within the adeno-associated virus (aav) sequences of the infectious recombinant plasmid psm620. studies of these mutants revealed at least three aav phenotypes. mutants with mutations between 11 and 42 map units were partially or completely defective for rescue and replication of the aav sequences from the recombinant plasmids (rep mutants). the mutants could be complemented by mutants with replication-positive phenotypes. the protein( ... | 1984 | 6086948 |
| requirement for either early region 1a or early region 1b adenovirus gene products in the helper effect for adeno-associated virus. | several adenovirus early genes act together to promote growth of the helper-dependent adeno-associated virus (aav). data from several laboratories have implicated adenovirus early regions 1a, 1b, 2a, and 4 in the helper effect, as well as the small rna polymerase iii transcript, virus-associated rna i. although a subset of these must participate directly in the aav life cycle, some may play an indirect role by influencing expression of the others. this paper is concerned particularly with the ro ... | 1984 | 6086952 |
| conformation takes precedence over sequence in adeno-associated virus dna replication. | deletion of an 11-base symmetrical sequence in the inverted terminal repetition of the adeno-associated virus 2 genome inhibits dna replication. substitution of either an 8- or a 12-base symmetrical sequence unrelated to the original 11-base sequence restores dna replication. | 1984 | 6504049 |
| autonomous parvovirus luiii encapsidates equal amounts of plus and minus dna strands. | autonomous parvoviruses are thought to uniquely encapsidate single-stranded dna of minus polarity. in contrast, the defective adeno-associated viruses separately encapsidate equal amounts of plus and minus dna strands. we reexamined the uniqueness of minus strand encapsidation for the autonomous parvoviruses. although we found that kilham rat virus and h-1 virus encapsidate varying but small amounts of complementary-strand dna, it was unexpected to find that luiii virus encapsidated equal amount ... | 1984 | 6694260 |
| analysis of proteins, helper dependence, and seroepidemiology of a new human parvovirus. | a new type of defective parvovirus, tentatively designated as adeno-associated virus type 5 (aav-5), is characterized as far as its proteins, its helper dependence, and its seroepidemiology are concerned. the protein analysis of aav-5 in polyacrylamide gels demonstrated the presence of three structural polypeptides, corresponding to vp 1, vp 2, and vp 3 of other aav types. the preparation of monoclonal antibodies against aav-5 permitted the analysis of viral structural antigen expression by usin ... | 1984 | 6200995 |