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preparation and characterization of a monoclonal antibody against the core protein vp7 of the 25th serotype of bluetongue virus.bluetongue virus (btv) is a member of the genus orbivirus, within the family reoviridae. the vp7 protein of btv is used for developing group-specific serological assays. to prepare monoclonal antibody (mab) against vp7 of the 25th serotype btv, the rna s7 encoding vp7 was cloned into prokaryotic expression vectors pet-28a (+) and pgex-6p-1 to generate recombinant plasmids. the recombinant protein vp7 was expressed in escherichia coli bl21 (de3), respectively. the results of sds-page revealed tha ...025897610
novel bluetongue virus in goats, corsica, france, 2014.during 2000-2013, 4 genotypes of bluetongue virus (btv) were detected in corsica, france. at the end of 2013, a compulsory btv-1 vaccination campaign was initiated among domestic ruminants; biological samples from goats were tested as part of a corresponding monitoring program. a btv strain with nucleotide sequences suggestive of a novel serotype was detected.025418049
seroprevalence of bluetongue disease in sheep in west and northwest provinces of iran.the objective of this study was to describe the seroprevalence rates of bluetongue virus (btv) in sheep in west and northwest provinces of iran. bluetongue virus, an economically important orbivirus of the reoviridae family, causes a hemorrhagic disease mainly in sheep and occasionally in cattle and some species of deer. bluetongue virus is transmitted between its mammalian hosts by certain species of biting midges (culicoides spp.) and it can infect all ruminant species. overall, 26 serotypes h ...025653797
the attenuation of bluetongue virus by serial passage through fertile eggs. 194720247520
the particle size of bluetongue virus as determined by ultrafiltration and ultracentrifugation. 194818863436
a preliminary report on the isolation and identification of the bluetongue virus from sheep in california. 195313044684
isolation of the bluetongue virus from texas sheep-culicoides shown to be a vector. 195413142963
susceptibility of bluetongue virus to magnamycin. 195413183868
bluetongue. 195413219048
losses in newborn lambs associated with bluetongue vaccination of pregnancy ewes. 195513251947
bluetongue. i. propagation of bluetongue virus of sheep in suckling hamsters. 195514371740
studies on bluetongue. iv. field trial of a modified live virus vaccine. 195613306670
complement fixation with antigens prepared from bluetongue virus-infected mouse brains. 195613319693
studies on bluetongue. v. distribution of bluetongue in the united states as confirmed by diagnostic tests. 195713416064
studies on bluetongue. iii. the development of a modified live virus vaccine employing american strains of bluetongue virus. 195713424922
the particle size of antigens prepared from bluetongue virus-infected suckling mouse brains. 195813580442
irradiation of cells in tissue culture. vii. studies on the susceptibility to bluetongue virus on radiation induced giant cells in vitro. 195913822281
the use of tissue culture-propagated bluetongue virus for vaccine preparation. 196113752422
pathology of bluetongue of sheep in california. 196113773253
studies on bluetongue - i. infectivity of the virus in the sheep ked, melophagus ovinus (l.). 196117649325
cytochemical changes of bluetongue virus in tissue cultures. 196214465988
preliminary investigations on insect transmission of bluetongue virus in sheep. 196314081454
current status of bluetongue in sheep. 196414156995
an ovine fetal encephalopathy caused by bluetongue vaccine virus. 196414219102
bluetongue associated with abnormalities in newborn lambs. 196414229979
isolation of an antigenic variant of bluetongue virus. 196414230694
clinical and pathologic features of bluetongue in sheep. 196414266906
preliminary bluetongue transmission with the sheep ked melophagus ovinus (l.).five experiments indicated that the sheep ked melophagus ovinus (l.), can transmit bluetongue virus (btv) in sheep. it was not determined whether these were mechanical or biological transmissions, although the results suggested mechanical transmission. sheep keds were manually transferred from a btv-host sheep to 18 susceptible test sheep. of these, 10 were positive (5 with mild reactions), 6 questionable, and 2 negative for btv. three of the mildly reacting sheep and 3 of the questionable sheep ...19654221988
the clinical and immunogenic response of sheep to oral and intradermal administrations of bluetongue virus. 19654956658
sensitivity of bluetongue virus to ether and sodium deoxycholate. 196514277649
studies on bluetongue ii. complement-fixing activity of ovine and bovine sera.sera of sheep and calves infected with the california type 10 and cyprus type 3 viruses of bluetongue were tested by the regular and modified direct complement-fixation tests. to obtain satisfactory complement fixation it was necessary to use the latter test. cross reactivity was found, therefore, the california type 10 antigen could be used in testing sera of animals infected with the cyprus virus.196514294803
bluetongue virus structure. 19664960306
concentration of bluetongue virus in experimentally infected sheep and virus identification by immune fluorescence technique. 19664967660
the adverse effect of some calf sera on the isolation and propagation of bluetongue virus in tissue culture. 19664964965
quantal and graded dose-responses of bluetongue virus: a comparison of their sensitivity as assay methods for neutralizing antibody. 19665220566
sensitivity of bluetongue virus to lipid solvents, trypsin and ph changes and its serological relationship to arboviruses. 19665223433
observations on bluetongue virus in the salivary glands of an insect vector, culicoides variipennis. 19665911888
multiplication of bluetongue virus in culicoides variipennis following artificial infection. 19665950517
observations on a strain of bluetongue virus by electron microscopy. 19664165343
studies on bluetongue. iv. studies of three strains in primary bovine foetal kidney cell cultures.three different strains of bluetongue virus were adapted to grow in primary bovine foetal kidney cell cultures. the cytopathic effects observed from the three strains were similar, and were characterized by shrinkage of cells and increased granularity. the specificity of the changes was confirmed by the fluorescent antibody technique. no significant immunological cross-reaction was detected by serum-virus neutralization tests from the strains studied.19674227046
studies on bluetongue. v. detection of the virus in infected materials by immunofluorescence.the fluorescent-antibody technique was employed for the detection of bluetongue virus in bovine foetal kidney cell cultures inoculated with tissues and blood from experimentally infected animals. in the first series, a total number of 79 inoculated suckling-mouse brains were examined, 36 as frozen sections alone and 43 as impression slides in conjunction with tissue culture inoculation of the same material. with the combined tissue culture immunofluorescent methods, 36 suspicious were detected b ...19674227047
studies on bluetongue. vi. animal transmission studies.the cyprus strain of bluetongue virus was successfully transmitted through six passages and the station strain through one passage in calves. although the animals developed no visible evidence of infection, viremia as shown by both passage and fluorescent antibody examination of infected foetal bovine kidney culture, and by serological conversion was nevertheless demonstrated. no enhancement of virulence for calves or sheep was shown following bovine passage. a ewe inoculated in late pregnancy w ...19674227048
action taken in queensland to prevent a possible introduction of bluetongue virus in cattle semen. 19676067735
cytopathologic changes and development of inclusion bodies in cultured cells infected with bluetongue virus. 19674965186
bluetongue virus infection: pathologic responses of nervous systems in sheep and mice.lesions caused by bluetongue virus infection of the central nervous systems of sheep and mice vary with age of the host. the character of the lesions appears to be influenced by the stage of immunological maturity of the infected animals.19674960482
plaque formation by bluetongue virus. 19674966975
bluetongue virus in sheep. intensification of the clinical response by previous oral administration. 19684968451
studies on the fluorescent and neutralizing antibodies to bluetongue virus in sheep. 19684974540
direct assay for bluetongue virus by intravascular inoculation of embryonating chicken eggs. 19684966159
clinical and serologic responses in vaccinated sheep given challenge inoculation with isolates of bluetongue virus. 19684966760
electron microscopic study of the formation of bluetongue virus. 19684328479
an improved method for the isolation and identification of bluetongue virus by intravenous inoculation of embryonating chicken eggs. 19684300798
experimental bluetongue disease in white-tailed deer.nine white-tailed deer and six sheep were experimentally exposed to the california btv-8 strain of bluetongue virus. the infections were fatal for seven of the nine deer. an additional deer died from exposure to an isolate of bluetongue virus from bighorn sheep. clinical signs and lesions of bluetongue in deer were described. the incubation period, signs and lesions of bluetongue and epizootic hemorrhagic disease of deer appear to be similar. virus isolations were made from the blood and a varie ...196815846890
immunodiffusion of bluetongue virus. 19694302278
purification and characterization of bluetongue virus. 19694306588
morphology of bluetongue virus. 19694306589
pathologic changes in placentas of ewes inoculated with bluetongue virus. 19694307344
ibaraki virus, an agent of epizootic disease of cattle resembling bluetongue. i. epidemiologic, clinical and pathologic observations and experimental transmission to calves. 19694309396
ibaraki virus, an agent of epizootic disease of cattle resembling bluetongue. ii. isolation of the virus in bovine cell culture. 19694309397
bluetongue virus-induced interferon synthesis. 19694340875
the application of improved techniques to the identification of strains of bluetongue virus. 19704340799
macromolecular synthesis in bluetongue virus infected cells. i. virus-specific ribonucleic acid synthesis. 19704340800
macromolecular synthesis in bluetongue virus infected cells. ii. host cell metabolism. 19704340801
failure to demonstrate in vitro as opposed to in vivo transcription of the bluetongue virus genome. 19704340802
some morphological features of bluetongue virus. 19704194857
bluetongue virus in white-tailed deer. 19704313262
serologic evidence of an etiologic role for bluetongue virus in hydranencephaly of calves. 19704314885
characterization of bluetongue virus ribonucleic acid.an improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (rna) component. double-stranded rna obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. the size of these components vary from 0.5 x 10(6) to 2.8 x 10(6) daltons, with a total molecular weight estimate of about 1.5 x 10(7) for th ...19704315158
observations on latent bluetongue virus infection in cattle. 19704316060
bluetongue virus in north american elk. 19704316480
ibaraki virus, an agent of epizootic disease of cattle resembling bluetongue. iv. physicochemical and serological properties of the virus. 19704319727
ibaraki virus, an agent of epizootic disease of cattle resembling bluetongue. 3. experimental inoculation of cell cultures, embryonated eggs and laboratory animals. 19704986398
bluetongue virus: (1) in pregnant white-tailed deer (2) a plaque reduction neutralization test.six white-tailed deer (odocoileus virginianus) were infected with bluetongue virus (bts, vaccinal strain) approximately one-third of the way through their gestation period. one deer died of bluetongue 21 days after inoculation. of the five surviving the infection, one had two mummified fetuses, and the others no fetuses upon euthanasia two weeks after term. fetuses were present in two control deer and in the one which died of bluetongue. a plaque reduction neutralization test for bluetongue viru ...197016512142
experimental viral-induced congenital encephalopathies. i. pathology of hydranencephaly and porencephaly caused by bluetongue vaccine virus. 19715095319
a preliminary survey of the epidemiology of bluetongue in kenya.a survey of the species composition and distribution of the culicoides midge populations at a range of sites where bluetongue is enzootic isolated a group of dominant species: c. cornutus, c. grahamii, c. magnus, c. milnei, c. pallidipennis and c. 23. dagger monthly light-trap sampling of culicoides showed that the population densities of the dominant species greatly increased after the rain seasons and that these species concentrated around flocks of sheep and cattle. the larval habitats of c. ...19714337112
the typing of bluetongue virus. 19714324624
hydranencephaly of calves associated with natural bluetongue virus infection. 19714325254
bluetongue virus: some relationships among north american isolates and further comparisons with ehd virus.seven isolates of bluetongue virus with different isolation histories and one isolate of the virus of epizootic hemorrhagic disease of deer were cloned by three consecutive plaquings in l-929 cells. the isolates were categorized on plaque size and margin. antisera to the bluetongue virus isolates were produced in calves and antiserum to epizootic hemorrhagic disease virus in deer. plaque reduction neutralization tests were done using the eight isolates and antisera to six of these. the isolates ...19714327870
a study of australian arboviruses resembling bluetongue virus. 19714130229
a comparison of bluetongue virus and ehd virus: electronmicroscopy and serology.bluetongue virus, bt(8), and the virus of epizootic hemorrhagic disease (ehd) of deer, nj-55, were plaque purified and compared electronmicroscopically and serologically. the latter included a plaque reduction neutralization test, the agar gel precipitin test, and the complement fixation test. the viruses were indistinguishable morphologically, but antigenically different. a plaquing technique was described for ehd virus.19714251410
experimental viral-induced congenital encephalopathies. ii. the pathogenesis of bluetongue vaccine virus infection in fetal lambs. 19714328760
host cell protein synthesis after infection with bluetongue virus and reovirus. 19714331734
a quantitative ultrastructural study of the development of bluetongue virus in madin-darby bovine kidney cells. 19714333711
physicochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus--a new taxonomic group. physiocochemical and serological studies. 19714333714
physicochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus--a new taxonomic group. electron microscopic studies. 19714333715
cattle and culicoides biting midges as possible overwintering hosts of bluetongue virus. 19714353606
bluetongue virus. 19714354734
effects of viral infection on nervous system development. ii. attempts to modify bluetongue-virus-induced malformations with cyclophosphamide and antithymocyte serum.treatment with cyclophosphamide and antithymocyte serum (ats) did not significantly increase the susceptibility of mice to bluetongue virus infections of the nervous system. cyclophosphamide actually inhibited virus growth in newborn and 2-week-old mice and, in newborn mice, diminished the severity of virus-induced pathologic sequelae. autoradiographic studies suggested this inhibition resulted from the effect of cyclophosphamide on the immature cells of the subventricular zone which were the ce ...19724342991
effects of viral infection on nervous system development. i. pathogenesis of bluetongue virus infection in mice.bluetongue vaccine virus was adapted to mice by serial intracerebral passage. the adapted virus was found to multiply selectively in immature cells of the subventricular zone of the forebrain and spread along cell migratory pathways to the olfactory bulbs, caudate/putamen, hippocampus and areas of cerebral cortex. symmetrical, mineralized, partially cavitated lesions developed in these areas as a sequela of the infection. infection of fetal mice led to more severe cerebral malformation, and infe ...19724342993
isolation and characterization of two types of bluetongue virus particles. 19724344192
interferon induction by bluetongue virus and bluetongue virus ribonucleic acid. 19724352039
studies on the in vitro and the in vivo transcription of the bluetongue virus genome. 19724352125
bluetongue virus in pronghorn antelope. 19724336799
bluetongue virus in goats. 19724340816
storage of bluetongue virus-infected culicoides variipennis. 19724340818
structure of the bluetongue virus capsid.seven polypeptides were found to be present in the capsid of the bluetongue virus (btv), four of which are major and three are minor components. this number and size distribution is the same as that found in reovirus, which has a similar segmented, double-stranded ribonucleic acid genome. in both viruses an excellent correlation is found between the molecular weights of certain genome segments and those of the polypeptides, suggesting a direct coding relationship between them. careful comparison ...19724117349
antigenic relationship between the virus of epizootic haemorrhagic disease of deer and bluetongue virus. brief report. 19724337550
animal model for human disease. hydranencephaly, porencephaly, cerebral cysts, retinal dysplasia, cns malformations. animal model: bluetongue-vaccine-virus infection in fetal lambs. 19724339926
control of transcription during the expression of the bluetongue virus genome. 19734372783
bluetongue virus in cattle: complement fixing antibody response and viremia in experimentally infected animals. 19734377944
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