Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| intracranial tumours in rats injected intracerebrally with murine sarcoma virus (msv), moloney's strain. preliminary report. | 1966 | 4293629 | |
| plasmacytomas occurring in the bones of rats injected intracerebrally with murine sarcoma virus (msv), moloney' strain. preliminary report. | 1966 | 5985024 | |
| [ultrastructural studies of a murine sarcoma virus (msv)]. | 1967 | 4295344 | |
| the differential action of neonatal thymectomy in mice infected with murine sarcoma virus-harvey (msv-h). | 1968 | 4302084 | |
| susceptibility of praomys (mastomys) natalensis to the murine sarcoma virus-harvey (msv-h). | 1968 | 4302086 | |
| replication of murine sarcoma virus-harvey (msv-h) in tissue cultures of virus-induced sarcomas. | 1968 | 4304098 | |
| [the role of immunity in the rejection of tumours induced by the msv virus. the effect of antithymocyte serums]. | 1968 | 4307754 | |
| transformation by murine sarcoma virus: fixation (deoxyribonucleic acid synthesis) and development. | transformation of rat embryo cells by murine sarcoma virus (msv) was contingent upon synthesis of deoxyribonucleic acid (dna) during the first 12 hr of infection. inhibition of dna synthesis by thymidine (20 mm) or cytosine arabinoside (0.1 mm) resulted in the protection of cells from transformation by msv. transient suppression of dna synthesis prior to infection or after a 12-hr delay had little effect on subsequent transformation, emphasizing the critical time period in in which dna synthesis ... | 1968 | 5701824 |
| biological and serological properties of viral particles from a nonproducer rat neoplasm induced by murine sarcoma virus (moloney). | the viral particles present in a nonproducer rat neoplasm induced by murine sarcoma virus (msv) moloney isolate, as detected by electron microscopy, were found to be biologically active on normal kidney cells of random-bred osborne-mendel rats. the virus is designated here as msv (0). msv (0) differs from other pseudotypes of msv in its host range, antigenicity, and interference pattern. | 1968 | 5725318 |
| rescue in vivo in adult mice of murine sarcoma virus (msv) from a nonproducer rat neoplasm. | 1969 | 5791815 | |
| biological activity and separation of a leukaemogenic virus from murine sarcoma virus-harvey (msv-h). | 1969 | 4310639 | |
| murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. | a continuous cell line of highly contact-inhibited cells (nih/3t3) has been developed from nih swiss mouse embryo cultures. its growth properties are similar to those of 3t3 and balb/3t3. although 3t3 is relatively insensitive to focus formation by murine sarcoma viruses, cloned lines of both nih/3t3 and balb/3t3 have been isolated that are highly sensitive to sarcoma virus focus formation and leukemia virus growth. the sensitivity and specificity are comparable to those found with primary embry ... | 1969 | 4311790 |
| enhancing effect of the murine sarcoma virus (msv) on the replication of the mouse hepatitis virus (mhv) in vitro. | 1969 | 4306053 | |
| [antigenic properties of a "graffi" pseudotype of the murine rhabdomyosarcoma virus (msv)]. | 1969 | 4306855 | |
| a spleen weight assay for murine sarcoma virus harvey (msv-h). | 1969 | 5346883 | |
| effectiveness of therapy with a multistranded synthetic polynucleotide complex against a murine sarcoma virus. | a known interferon stimulator, polyriboinosinic acid:polyribocytidylic acid (ri:rc), was shown to have a protective effect against a highly tumorigenic variant of the murine sarcoma virus (moloney) designated as (msv-pv). mice treated intraperitoneally with different regimens of ri:rc survived significantly longer than untreated controls. the treatment was effective whether the infection with msv-pv was intraperitoneal or intracerebral. the antiviral effectiveness of ri:rc was demonstrated by ma ... | 1969 | 5373679 |
| complementary nuclear rna's of murine sarcoma-leukemia virus complex in transformed cells. | the high molecular weight rna (s(20,w) = 69s) of murine sarcomaleukemia virus (msv-mlv) complex dissociated into 37s subunits when heated to 95 degrees c for 2 minutes. specific annealing tests revealed that the viral rna had complementarity with two heterogeneous rna species from the nuclei of cells (78 a1) transformed by and chronically infected with this virus complex. the two nuclear rna species that hybridized with the viral rna had sedimentation constants ranging from 31-36s and 18-22s, re ... | 1969 | 4318348 |
| [transplantable osteosarcoma caused by the "moloney strain of murine sarcoma virus (m-msv)" in osborne-mendel rats]. | 1970 | 4319060 | |
| immunity to murine sarcoma virus (msv)-induced tumors demonstrated by "in vivo" elimination of 51-chromium labeled tumor cells. | 1970 | 4322224 | |
| pathological effects in hamsters of murine sarcoma virus (msv harvey). | 1970 | 5420785 | |
| the role of dna synthesis in virus replication and the morphological transformation of normal mouse embryo cells by msv (moloney). | 1970 | 5466002 | |
| studies on hypodiploid and hyperdiploid virus-induced mouse leukemias and the vertical transmission of msv-moloney. | 1970 | 5538519 | |
| transformation of a cloned renal epithelial cell line by moloney murine sarcoma virus (m-msv). | 1970 | 4142389 | |
| trans-species rescue of defective genomes of murine sarcoma virus from hamster tumor cells with helper feline leukemia virus. | this report describes a trans-species rescue of defective msv genome with helper leukemia virus derived from cats. this rescue was achieved by in vitro co-cultivation of hamster tumor cells with feline embryo cells in the presence of helper feline leukemia virus (felv), or by inoculation of tumor cells into felv-infected newborn cats. the rescued focus-forming viruses produced foci in feline embryo cultures but not in cultures of mouse, rat, and hamster species. one isolate was tested and found ... | 1970 | 4313195 |
| host-range restrictions of murine leukemia viruses in mouse embryo cell cultures. | murine leukemia virus strains fall into three categories with respect to their ability to propagate in cells of national institutes of health (nih) swiss and balb/c mouse embryos. cultures of nih cells are 100- to 1,000-fold more sensitive to "n-tropic" strains than balb/c cell cultures, but are 30- to 100-fold less sensitive to "b-tropic" strains. some virus strains (dually tropic or "nb-tropic") propagate equally well in both cells. m-msv pseudotypes show the host-range characteristics of the ... | 1970 | 4317349 |
| infection and transformation of dog cells with a modified sarcoma virus. | dog-embryo cell cultures were infected with a mixture of feline leukemia virus (fellv) and a modified murine (moloney) sarcoma virus msv(fellv). morphological alteration of the cells was observed 3 days post infection. virus progeny from the infected cultures increased 50- to 100-fold in foci of cellular alteration on dog cell cultures pretreated with diethylaminoethyl-dextran, but only twofold on cat embryo cultures similarly treated. the numbers of foci produced corresponded to a dual infectio ... | 1970 | 18605433 |
| nucleic acid and proteins isolated from a strain of murine sarcoma virus (msv-o). | 1971 | 4327113 | |
| isolation of a rat-tropic helper virus from m-msv-o stocks. | 1971 | 4328487 | |
| rescue of murine sarcoma virus from a sarcoma-positive leukemia-negative cell line: requirement for replicating leukemia virus. | the nature of murine sarcoma virus (msv) "defectiveness" was investigated by employing an msv-transformed mouse 3t3 cell line which releases noninfectious virus-like particles. rescue kinetics of msv, observed after murine leukemia virus (mulv) superinfection of these "sarcoma-positive leukemia-negative (s + l -)" mouse 3t3 cells, consisted of a 9- to 12-hr eclipse period followed by simultaneous release of both msv and mulv with no evidence for release of infectious msv prior to the production ... | 1971 | 4332138 |
| clonal isolation of murine sarcoma virus (msv): characterization of virus produced from transformed cells. | 1971 | 4332977 | |
| the murine sarcoma virus (msv). | 1971 | 4333859 | |
| immune complex disease in chronic viral infections. | chronic viral infections of animals associated with immune complex disease resemble a number of human disorders. at present, on the basis of showing (a) virus, host antiviral antibody, and c3 deposited in a granular pattern along the glomerular capillary wall and in the mesangia and (b) virus-host ig (presumably antiviral antibody) complexes in the circulation, immune complex disease occurs in chronic murine infections with lcm, ldv, msv, and adm. in addition, granular deposits in the glomeruli ... | 1971 | 19867377 |
| deficiency of viral ribonucleic acid-dependent deoxyribonucleic acid polymerase in noninfectious virus-like particles released from murine sarcoma virus-transformed hamster cells. | virions from hamster cells transformed by a new strain of murine sarcoma virus (msv) appear to represent a form of msv deficient in one or more of the viral components necessary for infectivity. both the noninfectious virions and the sarcoma virus-transformed cells, which have no detectable replicating leukemia helper virus of either murine or hamster origin, are deficient in viral-type ribonucleic acid (rna)-dependent deoxyribonucleic acid (dna) polymerase activity (i.e., reverse transcriptase ... | 1972 | 4335663 |
| replication of radiation-induced murine leukemia virus in normal and transformed mouse cells. | autonomous radiation-induced leukemia virus (radlv) replication could be detected in mouse 3t3 cells by the development of interference with murine sarcoma virus (msv), the appearance of covert helper activity for defective msv, and by the induction of cytopathic effect type foci in msv-transformed, leukemia virus-negative (s+l-) cells. a chronic infection of either 3t3 or s+l- cells with radlv could be established. both radlv infectivity and helper activity were demonstrated in the same peak at ... | 1972 | 4335664 |
| immunological studies on viral polypeptide synthesis in cells replicating murine sarcoma-leukemia virus. | antibodies to disrupted murine sarcoma-leukemia virus (msv[mlv]) were used to study the synthesis of viral polypeptides in the transformed, virus-producing rat cell line 78a1. when cultures were labeled for 10 min with radioactive amino acids, about 9% of the total labeled proteins were precipitated with antiserum against purified msv(mlv), and 3 to 4% were precipitated with the same antiserum after it had been absorbed with an extract from uninfected rat cells. the difference is due to the pres ... | 1972 | 4342052 |
| major group-specific protein of rat type c viruses. | the major internal protein of a rat type c virus pseudotype of murine sarcoma virus, msv(ralv), was purified by isoelectric focusing (pi = 8.6) and used to prepare antibody in guinea pigs. the protein was identified by its reaction with antisera reactive with the mammalian type c virus group-specific (gs) antigenic determinant, gs-3. the guinea pig antisera mainly contained species-specific (gs-1) antibody for reactions in gel diffusion with other type c viruses were limited to those of rat orig ... | 1972 | 4117346 |
| cell-mediated reaction against tumors induced by oncornaviruses. i. kinetics and specificity of the immune response in murine sarcoma virus (msv)-induced tumors and transplanted lymphomas. | 1972 | 4128690 | |
| antigenic properties of murine sarcoma virus-transformed balb-3t3 nonproducer cells. | the isolation of clonal lines of murine sarcoma virus-transformed, non-producer balb/3t3 cells has provided a model system for determining whether rna tumor virus-transformed cells possess virus-specific transplantation antigens. msv nonproducer cells (k-234) were clonally derived from an inbred mouse cell line, balb/3t3. a parallel virus-producing cell line was obtained by infection of the msv nonproducer cells with rauscher leukemia virus. k-234 was much more tumorigenic than k-234(r). preimmu ... | 1972 | 4550769 |
| studies on tumorigenicity of rat bone tumor virus. (sd-msv-m) in mice, rats and hamsters. | 1972 | 4657994 | |
| lack of distinctive surface antigen on cells transformed by murine sarcoma virus. | some murine sarcoma virus (msv)-transformed mouse 3t3 cells contain the msv genome in the absence of infectious helper murine leukemia virus (mulv) and msv production. these cells, designated s+l- (sarcoma positive, leukemia negative), were analyzed for the presence of a possible msv-determined membrane antigen by the mixed hemadsorption test and in vitro lymphocyte cytotoxicity assay. two different serological approaches were used: (a) isoantibody-free sera were obtained by immunizing with msv ... | 1972 | 5043415 |
| the lymphocyte response to primary moloney sarcoma virus tumors in balb-c mice. definition of the active subpopulations at different times after infection. | adult balb/c mice were injected with moloney sarcoma virus (msv) after which the animals' lymphocytes were examined for activity against moloney leukemia virus (mlv) antigen-bearing target cells at 5-day intervals for 30 days. lymphocytes from these animals and appropriately matched controls were fractionated into b cell-deficient (primarily t cells) and t cell-deficient (primarily b cells) subpopulations. macrophages were removed using iron powder and magnetism. the unfractionated lymphocytes, ... | 1973 | 4709269 |
| prevention of murine sarcoma virus oncogenesis in offspring of immunized female mice. | 1973 | 4795530 | |
| tumour-associated transplantation antigens of neoplasms induced by a naturally occurring murine sarcoma virus (fbj-msv). | 1973 | 4516007 | |
| control of gene expression in rat cells transformed by a cold-sensitive murine sarcoma virus (msv) mutant. | 1973 | 4359760 | |
| properties of noninfectious and transforming viruses released by murine sarcoma virus-induced hamster tumor cells. | the cell culture lines htg2 and htg3 were established from a transplantable hamster tumor induced by a murine sarcoma virus (strain gz-msv) after 17 and 60 in vivo passages, respectively. the viruses released by these two cell lines markedly differ in morphology, antigenic composition, infectivity, transforming ability, and enzymatic activity. htg2 virions contained the sarcoma genome but were noninfectious for mouse and hamster cells (s+h-virus). htg3 virions transformed hamster but not mouse c ... | 1973 | 4359960 |
| recovery of the genome of murine sarcoma virus (msv) after infection of cells with nuclear dna from msv transformed non-virus producing cells. | 1973 | 4360277 | |
| non-infectious intracisternal a-type particles in a sarcoma-positive, leukemia-negative mouse cell line transformed by murine sarcoma virus (msv). | 1973 | 4362933 | |
| the histogenesis of tumours induced in golden hamsters by murine sarcoma virus-harvey (msv-h). | 1973 | 4364799 | |
| isolation of rd-114-like oncornavirus from a cat cell line. | a clone of cells derived from a continuous line of cat cells (ccc) spontaneously produced an rna c-type virus (ccc virus) which did not have the group-specific antigen of the standard strains of feline leukemia viruses but did have that of the rd-114 virus. single-hit infection of a virus yielding ccc cell with only the feline leukemia virus pseudotype of murine sarcoma virus [msv(felv)] resulted in the release of a pseudotype of msv coated with the ccc virus envelope. host range, transmission o ... | 1973 | 4123287 |
| transformation of rat liver cells with chicken sarcoma virus b77 and murine sarcoma virus. | rat liver cells in vitro were transformed with chicken sarcoma virus b77, giving rl(b77) cells, and with murine sarcoma virus (harvey), giving rl(msv) cells. rat liver cells transformed spontaneously in vitro were designated rl cells. in addition, the rl(msv) cell line was adapted for growth in culture fluid containing 25 mug of 5-bromodeoxyuridine per ml. all cell lines were tumorigenic in 1-wk-old rats. the number of cells needed for induction of tumor growth was 1,000-fold higher in the case ... | 1973 | 4347422 |
| infectivity and rna patterns as functions of high- and low-dilution passage of murine sarcoma-leukemia virus: evidence for autointerference within an oncornavirus population. | heterogeneity of buoyant density and rna content of virions of moloney murine leukemia-sarcoma complex [msv (mlv)] was the result of passage at low dilution. heterogeneous stocks revealed two major rna components in the population, with the smaller component, apparent mol wt 4 x 10(6) to 5 x 10(6), becoming predominant upon serial passage at low dilution. concomitantly, infectivity titers of both mlv and msv decreased upon serial passage at low dilution. msv (mlv) passaged at high dilution retai ... | 1973 | 4350709 |
| mutants of nonproducer cell lines transformed by murine sarcoma viruses. i. induction, isolation, particle production, and tumorigenicity. | a variety of cell mutants were obtained by a single 5'-bromodeoxyuridine (brdu) treatment of an nonproducer (np) cell line transformed by the kirsten strain of murine sarcoma virus (ki-msv). isolation procedures of these cell see pdf for structure mutants are described. the cell mutants obtained were classified by tumorigenic potential and shedding of type c virus particles. the cell mutants were classified into four groups: (a) tumorigenic, without particles; (b) tumorigenic, with type c partic ... | 1973 | 4352583 |
| dna polymerase of murine sarcoma-leukemia virus: lack of detectable rnase h and low activity with viral rna and natural dna templates. | kirsten murine sarcoma-leukemia virus (ki-msv[mlv]) was found to contain less rnase h per unit of viral dna polymerase than avian rous sarcoma virus (rsv). upon purification by chromatography on sephadex g-200 and subsequent glycerol gradient sedimentation the avian dna polymerase was obtained in association with a constant amount of rnase h. by contrast, equally purified dna polymerase of ki-msv(mlv) and moloney [mo-msv(mlv)] lacked detectable rnase h if assayed with two homopolymer and phage f ... | 1973 | 4357518 |
| ribonucleic acid components of murine sarcoma and leukemia viruses. | defective kirsten murine sarcoma virus was present as leukemia virus pseudotype [ki-msv(mlv)] in a 10- to 100-fold excess over its helper, kirsten murine leukemia virus (ki-mlv), when the two viruses were propagated in an nrk rat cell line. the s(omega,20) of the fastsedimenting rna complex of ki-mlv and of ki-msv-(mlv) was 62 s and 55 s, respectively. gel electrophoresis in buffered aqueous or formamide solution of the dissociated 62s rna complex of ki-mlv showed a single major peak of molecula ... | 1973 | 4357879 |
| murine virus-induced proteins synthesized by hamster tumor cells transformed by, but not producing, murine sarcoma virus. | the 8303 hamster tumor cells transformed by moloney strain of murine sarcoma virus (m-msv), but which do not produce virus, do contain murine virus-induced proteins. the virus-induced proteins within the cell were identified either as free proteins or in association with membranous material, including the plasma membrane. in addition, some were excreted by the 8303 hamster tumor cells into the growth medium. most virus-induced proteins were larger than 68,000 daltons, and they did not dissociate ... | 1974 | 4359302 |
| antigens of tumours induced by naturally occurring murine sarcoma virus (msv-fbj). ii. detection of cell-surface antigens by indirect membrane immunofluorescence. | 1974 | 4133783 | |
| baboon virus isolate m-7 with properties similar to feline virus rd-114. | a virus (m-7) isolated from baboon placental tissue demonstrates many similarities to endogenous feline virus rd-114. immunodiffusion analysis shows a group-specific antigen (gs-1) line of identity between m-7 and rd-114. anti-rd-114 dna polymerase igg inhibits m-7 polymerase by 57% compared to 97% for rd-114. m-7 virus has helper activity as demonstrated by rescue of murine sarcoma virus (msv) from sarcoma-positive leukemia-negative human amnion cells. the host range of the rescued m-7 pseudoty ... | 1974 | 4134740 |
| expression of the major internal viral polypeptide in cells transformed by wild-type and temperature-sensitive murine sarcoma virus. | phenotypic expression of the murine intraspecies and interspecies antigenic determinants of the major type c viral structural 30,000-dalton polypeptide, p30, was measured by radioimmunoassay inhibition in cell lines from different species. uninfected normal rat kidney (nrk) cells did not contain detectable levels of murine intraspecies and interspecies p30 antigen, whereas rat cells transformed by and producing murine sarcoma virus (msv)-moloney leukemia virus (m-msv-mulv) contained high levels ... | 1974 | 4139289 |
| revertants of mouse cells transformed by murine sarcoma virus: flat variants without a rescuable sarcoma virus from a clone of balb/3t3 transformed by kirsten msv. | 1974 | 4140213 | |
| sarcoma-virus-related rna sequences in normal rat cells. | a rat type c virus spontaneously activated from the nrk (normal rat kidney) cell line was found to have two major size classes of viral rna subunits sedimenting at 35 and 30 s. virus-producing cells contained both rna species, while normal "virus-free" rat cells contained primarily virus-specific 30s rna species. a dna transcript, specific for kirsten sarcoma virus, prepared from virus activated in nonproducer balb/c cells originally transformed by kirsten sarcoma virus and rendered specific for ... | 1974 | 4140505 |
| [proceedings: small molecular weight rna from a murine oncornavius: 85 rna from murine sarcoma virus (msv-m-mlv)]. | 1974 | 4141442 | |
| isolation and characterization of revertant cell lines. vi. susceptibility of revertants to retransformation by simian virus 40 and murine sarcoma virus. | the susceptibility of two classes of revertants of simian virus 40 (sv40)-transformed 3t3 cells to retransformation by sv40 or murine sarcoma virus (msv) was studied. both serum-sensitive and density-sensitive revertants are not retransformable by sv40. msv can transform both types of revertants. the msv-transformed revertants grow to high cell densities and form colonies when suspended in semi-solid methylcellulose medium, but are unable to grow in 1% calf serum. the msv-transformed revertants ... | 1974 | 4372404 |
| immunological responsiveness in rats infected with the soehner-dmochowski murine sarcoma virus (msv-sd). | 1974 | 4372740 | |
| comparative in vitro studies on effector cell diversity in the cellular immune response to murine sarcoma virus (msv)-induced tumors in mice. | 1974 | 4360862 | |
| leukemic mitochondria. i. acute myeloblastic leukemia. | quantitative and qualitative electron microscopic studies were performed on the mitochondria of leukemic myeloblasts in 3 patients with myeloblastic leukemia and in 3 patients with myelomonoblastic leukemia. in addition, to confirm the presence of virus-like particles within mitochondria and better understand the interaction between the virus and mitochondrion, a rat embryo tissue culture infected with msv-mlv (moloney sarcoma virus-moloney leukemia virus) was selected for detailed electron micr ... | 1974 | 4520328 |
| immunity to murine sarcoma virus induced tumours. 3. analysis of the cell populations involved in protection from lethal tumour progression of sublethally irradiated, msv inoculated, mice. | 1974 | 4547542 | |
| proceedings: "wild" type antigens of sarcomata induced by a natural amine sarcoma virus (msv-fbj). | 1974 | 4595171 | |
| evidence of suppressor cell activity in spleens of mice bearing primary tumors induced by moloney sarcoma virus. | spleens from moloney sarcoma virus (msv) tumor-bearing c57bl/6n mice contained four times the normal number of mononuclear cells and displayed a markedly elevated "spontaneous" (mitogen-independent) dna synthesis on a per cell basis. the number of macrophages were increased three-fold while there was a slight reduction in the percentage of t lymphocytes. the phytohemagglutinin (pha) response on a per cell basis of spleens from tumor-bearing mice was decreased about 90% when compared with normal ... | 1974 | 4598016 |
| tumor induction by murine sarcoma virus in akr and c58 mice. reduction of tumor regression associated with appearance of gross leukemia virus pseudotypes. | adult akr and c58 mice injected intramuscularly with murine sarcoma virus, moloney isolate (m-msv), developed high incidence of nonregressing local tumors. histologically, these tumors revealed the typical pleomorphism of m-msv sarcomas; in some cases, however, neoplastic tissue showed a nodular or diffuse growth of monomorphic myoblastlike cells, reminiscent of clonal aggregates. no depression of immune reactivity was found in m-msv-injected mice as evaluated by direct hemolytic plaque-forming ... | 1974 | 4608945 |
| antigens of tumours induced by naturally occurring murine sarcoma virus (msv-fbj). i. detection of group and type specific antigens by complement fixation. | 1974 | 4820943 | |
| intracellular viral rna species in mouse cells nonproductively transformed by the murine sarcoma virus. | the size and quantity of virus-specific rna in five non-virus-producing mouse cells transformed by the moloney isolate of murine sarcoma virus (msv) was determined. hybridization of rna from transformed cells with the [(3)h]dna product of the rna-directed dna polymerase of the murine sarcoma-leukemia virus was used to detect and quantitate virus-specific rna. the amount of virus-specific rna in non-virus-producing cells was less than one-sixth of that found in virus-producing cells. a striking c ... | 1974 | 4852536 |
| induction of virus ribonucleic acids in non-permissive msv-if+ cells infected with ndv. | 1974 | 4855927 | |
| revertants of mouse cells transformed by murine sarcoma virus. iii. metastable expression of virus functions in revertants retransformed by murine sarcoma virus. | 3t3fl cells transformed by the moloney murine sarcoma virus (msv) (sarcoma positive, leukemia negative, s+l- cells) segregate spontaneous flat revertants. these s-l- revertants do not release msv upon superinfection with mulv but are susceptible to a second cycle of single-hit msv transformation. the behavior and nature of second-cycle msv transformed revertants were followed by successive clonal generations. the second transformation by msv resulted in a number of metastable states. the simples ... | 1974 | 18620232 |
| purification and characterization of the dna polymerase and rnase h activities in moloney murine sarcoma-leukemia virus. | two rnase h (rna-dna hybrid ribonucleotidohydrolase, ec 3.1.4.34) activities separable by sephadex g-100 gel filtration were identified in lysates of moloney murine sarcoma-leukemia virus (msv). the larger enzyme, which we have called rnase h-i, represented about 10% of the rnase h activity in the virion. rnase h-i (i) copurified with rna-directed dna polymerase from the virus, (ii) had a sedimentation coefficient of 4.4s (corresponds to an apparent mol wt of 70,000), (iii) required mn-2+ (2 mm ... | 1975 | 46924 |
| cell-mediated immunity to moloney sarcoma virus in mice. i. analysis of antigens responsible for lymphocyte stimulation in regressor mice. | purified viruses, viral antigens, and cell extracts have been tested for their ability to stimulate protein synthesis by msv-m-1 regressor spleen cells from balb/c mice. immune, but not normal cells, responded to msv-m, but not to msv-g virus-1, and to the type-specific viral envelope glycoprotein from msv-m virus. extracts of mouse embryo fibroblasts transformed by either msv-m or msv-g, however, specifically stimulated msv-m regressor spleen cells. cells stimulated by different antigens, and b ... | 1975 | 47841 |
| a p60 polypeptide in the feline leukemia virus pseudotype of moloney sarcoma virus with murine leukemia virus p30 antigenic determinants. | a 60,000-dalton polypeptide (p60) has been identified in the feline leukemia virus (felv) pseudotype of moloney sarcoma virus [msv(felv)]. this polypeptide is present in the purified virus complex in concentrations greater than either the murine p30 or the feline p27. purified p60 crossreacts immunologically with murine p30 group antiserum and contains several interspecies determinants, whereas the group specific determinant of felv p27 is not detected. comparison of peptide fingerprints of p60 ... | 1975 | 49060 |
| antigenicity of carcinogen and viral induced sarcomas in inbred and random bred guinea pigs. | a tumor specific transplantation antigen (tsta) has been detected in a methylcholanthrene (mca) induced guinea pig tumor. it was possible to induce resistance to rechallenge with the tumor by immunization with irradiated cells in cfa. in contrast, the same technique failed to detect tsta in two viral (kirsten strain mouse sarcoma virus, ki-msv) induced guinea pig tumors; these results are similar to observations made with mouse ki-msv-induced tumors. transplantation studies with these tumors in ... | 1975 | 50348 |
| immunity to murine sarcoma virus induced tumours. iv. direct cellular cytolysis of 51cr labelled target cells in vitro and analysis of blocking factors which modulate cytotoxicity. | the antigen specific cell mediated cytotoxicity of msv immune spleen lymphocytes to 51cr labelled murine lymphoma cells was wholly abolished by pretreatment of the spleen cells with anti-theta antibody and complement. early during the immune response to msv the cytotoxic acitivity was inhibited by incubation of immune lymphocytes with "late progressor" or "early regressor" serum. immune lymphocytes at later times were more refractory to such inhibition by serum blocking factors. although unfract ... | 1975 | 50851 |
| properties of oncornavirus rna-directed dna polymerase, the rna template, and the intracellular products formed early during infection and cell transformation. | we have investigated three aspects of rna turmor virus replication and cell transformation: (1) the properties of the purified avian and mammalian viral rna-directed dna polumerase, (2) some characteristics of the viral 60-70s rna genome, 30-40s rna subunits and intracellular viral rna species, and (3) the interaction of the viral dna polymerase with its rna template early during infection and cell transformation by the murine sarcoma-leukemia virus (msv[mlv]). avian myeloblastosis virus (amv) c ... | 1975 | 50902 |
| characterization of murine sarcoma virus transformation of guinea pig cells and activation of an rna tumor-like virus from nonproducer guinea pig cells. | guinea pig embryo (gep) cells were transformed in vitro by the kirsten strain of mouse sarcoma virus (ki-msv). the transformed cells were found to release infectious virus continuously and produced high titers of group-specific (gs) complement-fixing (cf) antigen characteristics of the murine sarcoma-leukemia virus complex. foci of transformed cells were similar in appearance to those obtained with ki-msv in mouse and rat cells. the transformed cells produced rna dependent dna polymerase and typ ... | 1975 | 51621 |
| biochemical approach to bovine leukemia. | many bovine leukemic lymphocytes produce virus particles when kept in survival cultures in eagle's minimum essential medium supplemented with 20 percent of inactivated fetal calf serum. virus particles equilibrate at a density of 1.16 g/ml in sucrose gradients and at a density of 1.12 g/ml in metrizamide gradients. simultaneous detection tests show that a high molecular weight rna-reverse transcriptase complex exists in these viruses. hybridizations between total rna from bovine leukemic lymphoc ... | 1975 | 52980 |
| secondary specific immune response in vitro to msv tumor cells. | the interactions which occur between antigenic tumor cells and normal or immune lymphoid cells in a 3-day in vitro culture, have been studied with a murine sarcoma virus (msv)-induced tumor. the 3h-thymidine incorporation of lymphoma cells growing in suspension, and the radioactive-chromium release of freshly sampled lymphoma cells regularly added to the culture, have been compared to determine the part played by immune lymphoid cells in cytolysis and cytostasis of the tumor-cell population. the ... | 1975 | 53210 |
| mouse msv transformed cells resistant to 8-azaguanine. | mouse cells transformed by murine sarcoma virus were made resistant to 8-azaguanine. resistant cells and cell clones isolated from them were deficient in hypoxanthine-guanine phosphoribosyl transferase (hgprt) activity. they did not grow in hatg medium, did not incorporate labeled hypoxanthine, and had negligible hgprt activity. the resistance was genetically stable. the resistant cells were hyperdiploid and contained telocentric chromosomes only. the resistant cells as well as the progenitor ce ... | 1975 | 55981 |
| moloney sarcoma virus-induced tumors in athymic (nude) mice: growth pattern and antibody responses. | tumor growth and antibody production were evaluated in nude (nu/nu) mice and their heterozygous normal (+/nu) littermates after inoculation of moloney sarcoma virus (msv). sarcoma-bearing nude mice developed progressively growing tumors, whereas 53 percent of their normal counterparts showed tumor regressions. by indirect membrane fluorescence, significant amounts of igg antibody to msv could be detected in thymus-bearing but not in nude mice. | 1975 | 164570 |
| transformation of horse skin cells by type-c sarcoma viruses. | a horse skin cell line (e. derm, nbl-6, ccl-57) was susceptible to focus formation by the kirsten mouse sarcoma virus, feline sarcoma virus (st stain) and the msv pseudotypes with woolly monkey, gibbon monkey, rd-114, at-124, baboon placenta and murine xenotropic (balb/c 3t3 and c57l/jd) type-c viruses. foci were detected within 5 days after infection and the transformed cells continued to produce infectious virus and group-specific antigen of their respective type-c leukemia viruses. the transf ... | 1975 | 165152 |
| sarcoma-negative leukemia-positive transformed cell culture established from a murine sarcoma virus-induced rat bone tumor. | inoculation of the soehner-dmochowski isolate of the moloney strain of murine sarcoma virus (msv), designated msv-sd, consistently leads to the development of bone tumors in the susceptible new zealand black (nb) rats. two separate cell cultures have been established from 2 individual msv-sd-induced nb rat bone tumors. cells of 1 bone tumor culture, designated rbt-e, are in early in vitro passages. these cells form colonies in agar medium and take up 2-deoxy-d-[3h]glucose at a greatly enhanced r ... | 1975 | 167960 |
| evaluation of the cell-mediated immune response to murine sarcoma virus by (125i)iododeoxyuridine assay and comparison with chromium 51 and microcytotoxicity assays. | the cell-mediated immune response of c57bl/6 mice to murine sarcoma virus (msv) was examined by the [125i]-iododeoxyuridine release cytotoxicity assay using msv-induced sarcoma tissue culture cell lines as target cells. cellular cytotoxicity was detected as early as 3 days after virus inoculation. most mice assayed between 12 and 17 days after msv inoculation gave positive results with maximum levels of activity present on days 13 and 14. reactivity was frequently detected for up to 100 days aft ... | 1975 | 167966 |
| secondary cell-mediated cytotoxic response to syngeneic mouse tumor challenge. | when c57bl/6 mice previously immunized with murine sarcoma virus (msv) were challenged with a rauscher virus-induced lymphoma, rbl-5, a secondary cell-mediated cytotoxic response could be detected by the cr release cytotoxicity assay. the level and distribution of the secondary cytotoxic response was affected by the route of challenge. animals injected i.p. demonstrated a high level of cytotoxicity in the peritoneal exudate cells 3 days after challenge and subsequently cytotoxicity was detected ... | 1975 | 168259 |
| transformation of cultured rat liver cells by murine sarcoma viruses. | cultured liver cells (trl-2 cells), originating from a bd-6 rat, an inbred black rat strain, and possessing several marker enzymes, and characteristic ultrastructures of hepatic cells, were infected with murine sarcoma viruses (msv: kirsten and gazdar strains). on day 5-6 after virus inoculation, the monolayered epithelial cells focally began to pile up forming small polypoid projections, and on day 7 round-shaped cells were released from the polypoid foci. these cells first grew in a chain or a ... | 1975 | 169808 |
| human amnion and lung tissue culture systems for possible detection and study of human rna tumor viruses. | a human lung and an amnion cell line were identified as highly susceptible to transformation by the rhabdomyosarcoma-114 (rd-114) virus pseudotype of murine sarcoma virus (msv). msv transformation on these two cell lines demonstrated a) "one-hit" kinetics with an msv stock which contained a 100-fold excess of msv over its detectable associate rd-114 helper virus and b) only a slight increase (2x) in focus-forming titers by the addition of optimal concentrations of rd-114 helper virus. these find ... | 1975 | 169818 |
| control of reversion of moloney virus sarcoma virus transformed cells. | mouse cells transformed by the moloney murine sarcoma virus (msv) native to the species can give rise to revertants which are supersusceptible to a second cycle of transformation. the msv retransformed cells can give rise to several complex functional states even after several cycles of cloning: a) formation of sarcoma positive, leukemia negative (s+lminus) type cells of which some sublines may be inducible for msv by halogenated pyrimidines; b) detection of initially sminuslminus cells which sp ... | 1975 | 169824 |
| in vivo interactions between murine leukemia and sarcoma viruses. | experiments have been performed with the aim of elucidating the nature and the extent of the in vivo interactions between murine leukemia viruses (mulvs) and murine sarcoma virus (msv). balb/c and cba mice, injected neonatally with graffi or passage a gross viruses (mulv-gi, mulv-g), have been inoculated as young adults with murine sarcoma virus, moloney strain (msv-m). a higher percentage of nonregressing sarcomas appeared in these animals, sometimes accompanied simultaneously by leukemia. the ... | 1975 | 169829 |
| genetic control of oncogenesis by murine sarcoma virus moloney pseudotype. i. genetics of resistance in akr mice. | infection of moloney mouse sarcoma virus (m-msv) in adult mice of several inbred strains revealed that all strains except akr are highly susceptible to m-msv tumor development. f1 hybrids between akr and cba, dba/2 or nih mice are as resistant (93%) as the parental akr strain, which indicates that resistance is transmitted as a dominant character. first backcross mice to the susceptible parent show a 3:1 ratio of resistant to susceptible mice. this is the expected ratio for two segregating loci ... | 1975 | 170219 |
| genetic control of oncogenesis by murine sarcoma virus moloney pseudotype. ii. a dominant epistatic susceptibility gene. | we have shown in the preceding paper that akr mice are highly resistant to m-msv tumor development, and that resistance is transmitted as a dominant character. in the present studies the tumor-response pattern of f1 hybrids between resistant akr and susceptible strains (c57bl/6, balb/c and b10br) following injection with moloney mouse sarcoma virus (m-msv) resembles that of the non-akr parent. segregation is observed in first backcross (bc1) and f2 mice, and the segregation ratios up to bc3 mice ... | 1975 | 170220 |
| derivation of mouse sarcoma virus (kirsten) by acquisition of genes from heterologous host. | the technique of virus rna-cellular dna hybridization in solution with dna excess was used to compared the nucleotide sequences of the 70 s rna genome of the kirsten mouse sarcoma virus (ki-msv) with that of mouse erythroblastosis virus (mev) which gave rise to ki-msv after in vivo propagation in rat. it is suggested that a loss of about 30% of the genomic sequences of mev with a concomitant gain of roughly equal amounts of rat-specific sequences in a genetically stable recombinant state led to ... | 1975 | 170369 |
| murine xenotropic type c viruses i. distribution and further characterization of the virus in nzb mice. | the xenotropic mouse type c virus, originally detected in cultured embryo cells from new zealand black (nzb) mice, has been recovered from over 50 adult nzb animals and 15 nzb embryos. its presence is best detected by measuring its ability to rescue a murine sarcoma virus (msv) genome from a non-virus-producing msv-transformed rat cell. the virus can serve as a helper for replication of msv. it has a distinct type-specific coat and is a prototype for a third serotype of mouse type c viruses, nzb ... | 1975 | 170419 |
| two levels of restriction by mouse or cat cells of murine sarcoma virus coated by endogenous xenotropic oncornavirus. | mouse and cat cells were each examined for the mode of restriction of endogenous xenotropic oncornavirus. murine xenotropic helper virus (mux) and its pseudo-type of moloney murine sarcoma virus (msv(mux)) were grown in cat cells to high titre. mux alone did not replicate in any mouse cell tested including normal or transformed outbred swis 3t3 cells or sc-i cells, but did grow in a variety of other mammalian cells. msv(mux) was not able to achieve that intracellular state from which it could be ... | 1975 | 171336 |
| radiation-enhanced murine sarcoma virus genome rescue activity. | although the doses of x-ray (312-2,500 r) used for irradiation of cells caused impairment of dna synthesis and cell replication, co-cultivation of x-irradiated mulv- carrier cells with un-irradiated nonproducer cells of msv-induced tumour resulted in as much as 20-fold increase in msv retrieval compared with the un-irradiated control. the enhancement was apparent also as a 3-fold increase in the number of cells producing msv (infectious centers) in the co-cultivation plate. this suggested that t ... | 1975 | 173498 |
| inhibition of proliferation of lymphoma cells and t lymphocytes by suppressor cells from spleens of tumor-bearing mice. | we have recently demonstrated suppressor cells in spleens of mice bearing tumors induced by moloney murine sarcoma virus (msv) which were non-t cells and inhibited phytohemagglutinin molney (pha)-induced dna synthesis of syngeneic normal spleen cells. from the present study, the suppressor cells appeared to be macrophages since they were radioresistant, inactivated by carrageenan, and removed by adherence columns and an iron/magnet technique. we have also found that suppressor cells were still f ... | 1975 | 122986 |
| large round cell granulomas induced by murine sarcoma virus (moloney) in mouse brains. | the effect of murine sarcoma virus of moloney strain on central nervous system was examined morphologically in swiss mice of different age. a single intracranial inoculation of cell-free virus solution resulted in the induction of characteristic intracerebral granulomas in 82.8% of the newborn to 5 day-old group, in 71.4% of the 6 to 10 day-old group, and in 68.0% of the 11 to 20 day-old group. the mean latency periods to tumor recognition were 16.5, 21.1, and 33.5 days, respectively. the granul ... | 1975 | 128993 |